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Evaluation of phenotypic variability of seedlings obtained from open pollination of three varieties of the genus Hylotelephium 三个水仙属品种开放传粉幼苗表型变异的评价
IF 2 4区 农林科学 Q2 HORTICULTURE Pub Date : 2023-08-28 DOI: 10.2478/fhort-2023-0024
Oldřiška Sotolářová, R. Sotolář, M. Vachůn, R. Pokluda, J. Uher
ABSTRACT This article reflects on the conventional open pollination breeding method of the genus Hylotelephium. Six-year-old seedlings were evaluated and compared with the mother plants for 3 years. A total of 1 063 seedlings were evaluated (54 in the H. ‘Red Cauli’, 90 descendants in the H. ‘Xenox’, 919 descendants in the H. ‘Purple Emperor’). This study deals with six evaluated characters of vegetative parts of plants (habit, height and width of plants, length and width of leaves and the colour of the upper side of the leaves). The most significant phenotypic variability was confirmed for habit, plant height, leaf colour in summer and leaf length in population H. ‘Xenox’ and for spring colouration of lower leaves in population H. ‘Red Cauli’. The highest degree of variability was found for plant height, where the monitored populations split into a total of seven different sizes. On the contrary, the lowest degree of variability was demonstrated for the length and width of leaves, where all populations showed shorter and narrower leaves compared to the mother plants, with minimal differences.
摘要本文对传统的水蛭属开放传粉育种方法进行了反思。对6年幼苗进行评价,并与母株进行3年比较。共对1 063株幼苗进行了评价,其中“红茎”54株,“Xenox”90株,“紫帝”919株。本文研究了植物营养部分的6个评价性状(习性、植物的高度和宽度、叶片的长度和宽度以及叶片上部的颜色)。表型变异最显著的是习性、株高、夏季叶色和叶长,以及春季红茎叶的下部叶色。植物高度的变异程度最高,被监测的种群分为七种不同的大小。相反,叶片长度和宽度的变异程度最低,所有种群的叶片都比母株短、窄,差异极小。
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引用次数: 0
Research on preparing seedling substrates using edible mushroom waste and application 食用菌废弃物制备育苗基质及应用研究
IF 2 4区 农林科学 Q2 HORTICULTURE Pub Date : 2023-08-05 DOI: 10.2478/fhort-2023-0020
Qi Liu, Sheng Wang, Jingyu Zhang, Tao He, Wen Chen, Xuanyue Zhao, L. Bao, Naiming Zhang
ABSTRACT To improve the utilisation of mushroom residue waste resources and identify a replaceable matrix material for peat, 10 different formula substrates and three commercially available substrates were examined to determine their physiochemical properties. Furthermore, the correlation and influence of these physicochemical properties on the substrates were explored. Pot experiments were conducted using Brassica campestris L. ssp. chinensis Makino var. communis Tsen et Lee (cabbage), Brassica chinensis L. (pakchoi), Cucumis sativus L. (cucumber), and Cucurbita moschata Duch. ex-Poiret (pumpkin). The results showed that the matrix was most significantly affected by water-holding porosity, aeration porosity, air-water ratio, total porosity, pH, electrical conductivity (EC), available phosphorous (AP), and available potassium (AK). The random forest (RFF) model indicated that pH and total nitrogen (TN) had the strongest influence on the plant height and stem diameter of the cabbage. Moreover, pH, water-holding porosity, and total porosity most significantly influenced the plant height, stem diameter, and seedling vigour index. AP and air-water ratio substantially affected the root length and root-to-crown ratio of the cucumbers, while EC, air-water ratio, and AP influenced the stem diameter, root length, and seedling vigour index of the pumpkin most. The biological characteristics of the four vegetables during the pot experiment indicated that the overall effect of the 10 substrates supplemented with perlite and vermiculite was better than in the basic group. Of these, T2 (mushroom waste: sawdust: catalyst: vermiculite = 8:2:5:5) displayed the best result and could be used as an alternative for peat seedling.
摘要为了提高蘑菇渣废弃物资源的利用率,寻找一种可替代的泥炭基质材料,研究了10种不同配方基质和3种市售基质的理化性质。此外,还探讨了这些物理化学性质对底物的相关性和影响。以油菜为原料进行盆栽试验。chinensis Makino var. communis Tsen et Lee(白菜)、Brassica chinensis L.(小白菜)、Cucumis sativus L.(黄瓜)和Cucurbita moschata Duch。ex-Poiret(南瓜)。结果表明,基质受持水孔隙度、通气性孔隙度、气水比、总孔隙度、pH、电导率(EC)、有效磷(AP)和有效钾(AK)的影响最为显著。随机森林(RFF)模型表明,pH和TN对白菜株高和茎粗的影响最大。pH、持水孔隙度和总孔隙度对株高、茎粗和幼苗活力指数的影响最为显著。有机磷和空气水比对黄瓜的根长和根冠比影响较大,而有机磷、空气水比和有机磷对南瓜的茎粗、根长和幼苗活力指数影响最大。盆栽试验期间4种蔬菜的生物学特性表明,10种基质中添加珍珠岩和蛭石的总体效果优于碱性组。其中,T2(蘑菇渣:锯末:催化剂:蛭石= 8:2:5:5)效果最好,可作为泥炭苗的替代基质。
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引用次数: 0
The effects of organic and conventional fertilization on oregano (Origanum onites L.) yield and quality factors 有机施肥和常规施肥对牛至产量和品质因素的影响
IF 2 4区 农林科学 Q2 HORTICULTURE Pub Date : 2023-06-01 DOI: 10.2478/fhort-2023-0016
F. Asri
ABSTRACT Oregano (Origanum onites L.) is an important medicinal and aromatic plant. The use of sector and economic value of oregano are determined by quality in world trade. This study was conducted during 2019–2021 to determine the effects of chemical and organic fertilizers on yield and quality of O. onites L. The experiment followed a randomized complete block design with six treatments and four replications, i.e., control (C: untreated plants), chemical fertilizer (F) (150:90:100 kg · ha−1), farmyard manure (FYM), chicken manure (CM), vermicompost (VC) and spent mushroom compost (SMC) (the objective was to obtain 150 kg N · ha−1 for organic fertilizer). Fresh yield increased by 3.36%–11.44% and 5.61%–13.59% with organic fertilization as compared with the control in both years while it increased by 22% and 19.0% with chemical fertilization. FYM and SMC were more effective in fresh yield among organic fertilizers. Essential oil (EO) increased by 18.8%–50.1% and 2.94%–19.85% with fertilization as compared with the control in both years. EO yield was significantly increased by fertilization, and CM was in the lead with direct effect, while VC and FYM had residual effects. The applications increased the carvacrol and thymol content of the EO compared with the control. VC was the most effective application for total flavonoid content, antioxidant activity and plant nutritional status, especially for nitrogen, phosphorus, potassium and calcium concentrations. The results showed that chemical and organic fertilizers positively affected the yield, EO content and nutritional status of O. onites.
牛至是一种重要的药用芳香植物。牛至的行业用途和经济价值取决于世界贸易中的质量。本研究于2019-2021年进行,旨在确定化学肥料和有机肥料对O.onites L.产量和质量的影响。实验采用随机完全区组设计,共有六个处理和四个重复,即对照(C:未处理的植物)、化学肥料(F)(150:90:100 kg·ha−1)、农家肥(FYM)、鸡粪(CM),蚯蚓堆肥(VC)和废蘑菇堆肥(SMC)(目的是获得150 kg N·ha−1的有机肥料)。有机肥和化学施肥分别比对照增产3.36%~11.44%和5.61%~13.59%,化学施肥分别增产22%和19.0%。在有机肥料中,FYM和SMC对鲜产量的影响较大。与对照组相比,施肥前后精油含量分别增加18.8%-50.1%和2.94%-19.85%。施肥显著提高了EO产量,CM在其中起主导作用,VC和FYM有残留作用。与对照组相比,应用增加了EO中香芹酚和百里酚的含量。VC对总黄酮含量、抗氧化活性和植物营养状况最有效,尤其是对氮、磷、钾和钙浓度最有效。结果表明,化学肥料和有机肥料对燕麦产量、EO含量和营养状况均有积极影响。
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引用次数: 0
Phytochemicals from Phillyrea latifolia L. leaves and fruit extracted with various solvents: Their identification and quantification by LC-MS and antihyperglycemic effects 不同溶剂提取的苦参叶和果实的化学成分:LC-MS鉴定、定量及降糖作用
IF 2 4区 农林科学 Q2 HORTICULTURE Pub Date : 2023-06-01 DOI: 10.2478/fhort-2023-0018
Ebru Aydın
ABSTRACT Phillyrea latifolia L. is a type of shrubland, which is widely known as mock privet, and belongs to the Oleaceae family. The objective of this study was to compare and assess the phytochemical composition, antioxidant and antidiabetic activities of ethyl acetate, methanol and aqueous extracts of the fruit and leaves of P. latifolia L. Phenolics were analysed by detecting individual bioactive compounds using an LCMS-2020 quadrupole mass spectrometer and by calculating total phenolic content (TPC). For the first time, the antioxidant and antidiabetic activities of both leaves and fruit were determined using DPPH radical scavenging. The aqueous extract was indicated to have higher antioxidant activities than ethyl acetate and methanol extracts. The individual constituents within the different extracts for both fruit and leaves were detected as the luteolin-7-O-glucoside in the ethyl acetate (854 μg · g–1 and 1,098 μg · g–1), methanol (1,241 μg · g–1 and 2,136.43 μg · g–1) and aqueous (509 μg · g–1 and 898.23 μg · g–1) extracts, respectively. Extractions of ethyl acetate and methanol demonstrated stronger inhibitory activity against human salivary α-amylase than the aqueous extract of both parts of the mock privet. Similarly, extraction of ethyl acetate from the leaves and fruit of the mock privet indicated significantly better inhibitory activity than the methanol and aqueous extracts, respectively, for the inhibition of α-glucosidase activity. This study indicates that both fruit and leaves of mock privet may use as a potential source of natural biomolecules to promote healthy activities.
宽叶七弦草(Phillyrea latifolia L.)是木犀科的一种灌木林,被广泛称为假女贞。本研究的目的是比较和评估宽叶P.latifolia L.果实和叶片的乙酸乙酯、甲醇和水提取物的植物化学成分、抗氧化和抗糖尿病活性。通过使用LCMS-2020四极质谱仪检测单个生物活性化合物并计算总酚含量(TPC)来分析酚类化合物。首次用DPPH自由基清除法测定了叶片和果实的抗氧化和抗糖尿病活性。水提取物比乙酸乙酯和甲醇提取物具有更高的抗氧化活性。在乙酸乙酯(854μg·g-1和1098μg·g–1)、甲醇(1241μg·g-1和2136.43μg·g/1)和水性(509μg·g.1和898.23μg•g-1)提取物中,检测到果实和叶片不同提取物中的单独成分为木犀草素-7-O-葡萄糖苷。乙酸乙酯和甲醇提取物对人类唾液α-淀粉酶的抑制活性比模拟女贞两部分的水提取物更强。类似地,从模拟女贞的叶子和果实中提取乙酸乙酯,在抑制α-葡萄糖苷酶活性方面分别比甲醇和水提取物表现出更好的抑制活性。这项研究表明,模拟女贞的果实和叶片都可以作为天然生物分子的潜在来源来促进健康活动。
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引用次数: 0
Impacts of different planting times on fruit quality and some bioactive contents of different strawberry cultivars 不同种植时间对不同草莓品种果实品质及部分生物活性成分的影响
IF 2 4区 农林科学 Q2 HORTICULTURE Pub Date : 2023-06-01 DOI: 10.2478/fhort-2023-0017
Nafiye Unal, V. Okatan, Jale Bilgin, I. Kahramanoğlu, H. S. Hajizadeh
ABSTRACT Strawberry fruit quality traits can be affected by genotype-environment interactions, which determine the consumer acceptance of fruits. This factorial experiment was based on completely randomised blocks (RCBD) with two planting dates (5 and 20 April) and cultivars (‘Albion’, ‘San Andreas’ and ‘Portola’) of strawberry with three replications, and some of pomological and qualitative factors of berry were investigated at harvest. The results showed no significant difference on fruit width, length, weight and firmness, while the plant yield and stem diameter (SD) were affected by different cultivars and planting dates. The minimum SD (19.80) was recorded in cv. ‘Portola’ in the planting date of 20 April. Although the lowest fruit yield was achieved in cv. ‘Portola’ at both planting dates, it had the highest titratable acidity (TA) (0.83%) at the first planting date (5 April). The amount of soluble solid concentration (SSC), electrolyte conductivity (EC), pH, TA and chlorophyll were not affected by cultivar and planting dates. The effect of planting dates and cultivar had no significant effect on berry colour. The highest total phenolic (275.44 mg GAE · 100 mL-1 FW) was recorded in cv. ‘San Andreas’ on the planting date of 20 April, while the lowest value (251.22 mg GAE · 100 mL-1 FW) was recorded in cv. ‘Portola’ on the planting date of 5 April. In general, it is suggested that the least fruit yield in strawberry cv. ‘Portola’ can be correlated with the least SD of the cultivar.
摘要草莓果实的品质性状受基因型与环境相互作用的影响,而基因型和环境相互作用决定了消费者对果实的接受程度。该因子实验基于两个种植日期(4月5日和20日)的完全随机区组(RCBD)和三个重复的草莓品种(“Albion”、“San Andreas”和“Portola”),并在收获时研究了浆果的一些油脂学和定性因素。结果表明,果实宽度、长度、重量和硬度没有显著差异,而不同品种和种植日期对植株产量和茎粗(SD)有影响。最小SD(19.80)记录在4月20日种植的“Portola”品种中。尽管“Portola”在两个种植日期的果实产量都最低,但在第一个种植日期(4月5日),它的可滴定酸度(TA)最高(0.83%)。可溶性固形物浓度(SSC)、电解质电导率(EC)、pH、TA和叶绿素的含量不受品种和种植日期的影响。种植日期和品种对浆果色泽的影响不显著。总酚含量最高(275.44 mg GAE·100 mL-1 FW)记录在4月20日的“San Andreas”号栽培品种中,而最低值(251.22 mg GAE•100 mL-1 FW)记录于4月5日的“Portola”号种植品种中。一般来说,认为“Portola”草莓品种的最小果实产量可能与该品种的最小SD相关。
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引用次数: 1
Effect of foliar application of zinc on annual productivity, foliar nutrients, bioactive compounds and oxidative metabolism in pecan 叶面施锌对山核桃年生产力、叶面养分、生物活性物质和氧化代谢的影响
IF 2 4区 农林科学 Q2 HORTICULTURE Pub Date : 2023-05-20 DOI: 10.2478/fhort-2023-0014
D. Ojeda-Barrios, Oscar Cruz-Álvarez, E. Sánchez-Chávez, J. P. Ciscomani-Larios
ABSTRACT Pecan nut production is quite commonly limited by zinc (Zn) deficiency. Here, we evaluate the response in terms of the concentrations of non-structural carbohydrates, yield components, foliar nutrient levels and oxidative metabolism in young ‘Western Schley’ pecan nut trees in response to foliar applications of 200 mg · L−1 of Zn as one of the following: ZnSO4, Zn-EDTA, ZnO nanoparticles (NPs) or the proprietary product ‘nitrazinc’ (NZN) (the control). Across two consecutive growing seasons, the spraying of Zn in these various forms helped maintain the foliar concentrations of non-structural carbohydrates, foliar nutrients (total-N, Ca2+ and Mg2+) and the kernel percentage of nuts. Likewise, trees sprayed with ZnSO4 maintained the concentrations of Zn in the leaflets across seasons. On the other hand, Zn-EDTA decreased the concentration of chlorophyll and total carotenoids. In general, leaflets treated with ZnSO4, Zn-EDTA and ZnO NPs reduced their oxidative metabolism. Sources of Zn – such as ZnSO4 – are commercially viable alternatives suitable for increasing the performance of some parameters associated with the yield and quality of nuts in pecan. It would be worthwhile to determine the optimal Zn dose rates for the various pecan cultivars in common use and also to increase our understanding of the physiological and biochemical changes associated with foliar Zn applications.
果胶的生产通常受到锌缺乏的限制。在这里,我们根据非结构碳水化合物的浓度、产量成分、叶片营养水平和氧化代谢来评估年轻的“西施利”山核桃树对200 mg·L−1锌的响应,锌是以下物质之一:ZnSO4、Zn-EDTA、ZnO纳米颗粒(NP)或专有产品“nitrazinc”(NZN)(对照)。在连续两个生长季节,以这些不同形式喷洒锌有助于保持非结构碳水化合物、叶面营养素(总氮、Ca2+和Mg2+)的叶面浓度和坚果的籽粒百分比。同样,喷洒ZnSO4的树木在不同季节保持小叶中的锌浓度。另一方面,Zn-EDTA降低了叶绿素和总类胡萝卜素的浓度。通常,用ZnSO4、Zn-EDTA和ZnO-NP处理的小叶降低了它们的氧化代谢。锌的来源,如ZnSO4,是商业上可行的替代品,适用于提高与山核桃坚果产量和质量相关的一些参数的性能。确定各种常用山核桃品种的最佳锌剂量率是有价值的,也有助于加深我们对与叶面施用锌相关的生理和生化变化的理解。
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引用次数: 1
Hot and cold drying of edible flowers affect metabolite patterns of extracts and decoctions 食用花卉的冷热干燥会影响提取物和煎剂的代谢产物模式
IF 2 4区 农林科学 Q2 HORTICULTURE Pub Date : 2023-05-12 DOI: 10.2478/fhort-2023-0015
S. Demasi, M. Caser, V. Scariot
ABSTRACT Edible flowers are rich in bioactive compounds and pigments that are on increasing demand in nutraceutical, medicinal, food, cosmetic and dyeing industries. This study evaluated the anthocyanins, phenolics and antioxidant activity of eight edible flowers (Bellis perennis L., Centaurea cyanus L., Dianthus carthusianorum L., Lavandula angustifolia Mill., Primula vulgaris Huds., Rosa canina L., Rosa pendulina L. and Viola odorata L.) after 24 h of hot drying (50 °C; natural convection stove) or cold drying (CD) (22 °C; heat-pump drying system). The hot-dried and cold-dried materials were then used to prepare ultrasound-assisted extracts, which are used as food additives or as a source of natural colourants, or decoctions (DECs), which are used for herbal teas. The edible flower UAEs and DECs had high amounts of anthocyanins (up to 3,284.6 mg C3G · 100 g-1), phenolics (up to 9,034 mg · 100 g-1) and antioxidant activity (866 μmol TE · g-1, 4,901 mmol Fe2+ · kg-1), with roses having the highest amount. The anthocyanin content and phenolic profile (flavonols, benzoic acids, cinnamic acids and flavanols) of edible flowers are found to be affected by drying and extraction methods. For decoctions, CD was found to be the most efficient drying method for all the parameters. For UAEs, CD resulted in a higher anthocyanin content, while hot drying resulted in a higher phenolic content, particularly flavanols. Overall, the studied edible flower extracts and decoctions can contribute to healthy and coloured substances, which can be used for designing innovative natural products. Rosa pendulina and R. canina are the most promising sources.
摘要食用花卉富含生物活性化合物和色素,在营养、医药、食品、化妆品和染料行业的需求越来越大。本研究评价了8种可食用花卉(Bellis perennis L.、Centaurea cyanus L.、Dianthus cartusianorum L.、Lavandula angustifolia Mill.、Primula vulgaris Huds.、Rosa canina L.、Rosa pendulina L.和Viola odorata L.)在热干燥(50°C;自然对流炉)或冷干燥(CD)(22°C;热泵干燥系统)24小时后的花青素、酚类物质和抗氧化活性。然后,将热干燥和冷干燥的材料用于制备超声波辅助提取物,该提取物用作食品添加剂或天然着色剂的来源,或用于草药茶的煎剂(DEC)。食用花UAEs和DECs具有高含量的花青素(高达3284.6 mg C3G·100 g-1)、酚类物质(高达9034 mg·100 g-1)和抗氧化活性(866μmol TE·g-1,4901 mmol Fe2+·kg-1),其中玫瑰含量最高。食用花的花青素含量和酚类物质(黄酮醇、苯甲酸、肉桂酸和黄烷醇)受到干燥和提取方法的影响。对于煎剂,CD被发现是所有参数下最有效的干燥方法。对于UAE,CD导致较高的花青素含量,而热干燥导致较高的酚类含量,尤其是黄烷醇。总的来说,所研究的可食用花卉提取物和煎剂可以产生健康和着色的物质,可用于设计创新的天然产品。下垂蔷薇和犬齿蔷薇是最有前景的来源。
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引用次数: 2
The effect of application of effluent water on sage (Salvia officinalis L.) yield and quality in lysimeters 污水处理对鼠尾草产量和品质的影响
IF 2 4区 农林科学 Q2 HORTICULTURE Pub Date : 2023-05-05 DOI: 10.2478/fhort-2023-0013
N. Valkovszki, M. Jancsó, Á. Székely, T. Szalóki, Ildikó Kolozsvári, Szilvia Tavaszi-Sárosi, Á. Kun
ABSTRACT Cultivation of medicinal plants with the irrigation of agricultural effluents might be of great importance to save fresh water resources, extend cultivation area and increase economic feasibility. We investigated the effects of saline fish farm effluent water, diluted and gypsum-supplemented effluent, and natural freshwater as a control irrigation in lysimeters. Beside plant growth parameters, macronutrient and sodium content and the amount and composition of essential oil of sage plants were measured. Significant differences among irrigation treatments were found in plant height, SPAD value and essential oil content. Seasonal variation was also observed on plant height, nutrient content of the leaves and the total essential oil content. The essential oil components characterised by the highest levels of availability were detected as α-thujone, camphor, ß-thujone, 1,8-cineol and ledol. Under effluent irrigation, the concentrations of - and ß-thujone increased slightly; only camphene, trans-sabinole and caryophyllene-oxide changed significantly. The other main components remained stable. Our analysis of the response of sage to the input of effluent provides a reasonable ground for recommending the utilisation of saline effluent water from intensive fish farming in sage production, thus preventing the wastage of valuable water resources.
利用农业废水灌溉栽培药用植物,对节约淡水资源、扩大种植面积、提高经济效益具有重要意义。我们研究了盐场废水、稀释和石膏补充废水以及天然淡水作为对照灌溉的效果。除测定植物生长参数外,还测定了鼠尾草植物的常量营养素和钠含量以及精油的含量和组成。不同灌溉处理在株高、SPAD值和挥发油含量上存在显著差异。株高、叶片养分含量和总挥发油含量也有季节变化。有效度最高的精油成分为α-图琼、樟脑、ß-图琼、1,8-桉叶油醇和ledol。出水灌溉下-和ß-图琼酮浓度略有升高;只有樟烯、反式松香素和石竹烯-氧化物变化显著。其他主要成分保持稳定。我们分析了鼠尾草对污水输入的反应,为建议在鼠尾草生产中使用集约化养鱼场排出的含盐污水提供了合理的依据,从而防止了宝贵水资源的浪费。
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引用次数: 1
Optimisation of ultrasonic-assisted extraction and biological activity of total flavonoids from leaves of Murrayae exotica using response surface methodology 利用响应面法优化超声辅助提取海参叶总黄酮的工艺及生物活性
IF 2 4区 农林科学 Q2 HORTICULTURE Pub Date : 2023-04-25 DOI: 10.2478/fhort-2023-0010
Yao Wen, Man Liu, Xueying Mai
ABSTRACT Murrayae exotica is a traditional Chinese medicine widely grown in southeast China. A Box–Behnken design of response surface methodology was employed to further optimise ultrasonic-assisted extraction conditions for M. exotica leaves total flavonoids (MELTF). The results showed that the optimised extraction conditions were an ultrasonic power of 240 W, an ultrasonic temperature of 60 °C, a solvent concentration of 76%, an ultrasonic time of 55 min and a liquid–solid ratio of 22 mL · g−1. Under these conditions, 8.59 ± 0.34 mg · g−1 was achieved as the mean experimental value of extraction yield, which amounts to 2.56 times that of ethanol leaching extraction. As compared to MELTF, MELTF after purification (MELPTF) had a higher purity of 9.96%, which was nearly nine times higher than that of MELTF (1.26%). As compared to MELTF, MELPTF had higher α-glucosidase and α-amylase inhibitory activities as well as DPPH· and ABTS·+ scavenging activities with IC50 values of 0.021, 0.094, 0.245 and 0.113 mg · mL−1, which are 1.33, 2.12, 3.17 and 1.78 times higher than those of MELTF (0.028, 0.199, 0.777 and 0.201 mg · mL−1). The study thus demonstrates the eligibility of MELPTF to be considered as a multifunctional bioactive ingredient having potential applications in anti-hyperglycaemic pharmaceutical formulation and as an antioxidant in functional foods.
野村是一种广泛生长在中国东南部的中药。采用响应面法的Box-Behnken设计,进一步优化超声辅助提取野荆叶总黄酮的工艺条件。结果表明,最佳提取条件为超声功率240 W,超声温度60℃,溶剂浓度76%,超声时间55 min,液固比22 mL·g−1。在此条件下,提取得率的平均实验值为8.59±0.34 mg·g−1,是乙醇浸提的2.56倍。与MELTF相比,纯化后的MELTF (MELPTF)纯度为9.96%,是MELTF(1.26%)的近9倍。与MELTF相比,MELPTF具有更高的α-葡萄糖苷酶和α-淀粉酶抑制活性以及DPPH·和ABTS·+清除活性,IC50值分别为0.021、0.094、0.245和0.113 mg·mL−1,分别是MELTF(0.028、0.199、0.777和0.201 mg·mL−1)的1.33、2.12、3.17和1.78倍。因此,该研究证明了MELPTF作为一种多功能生物活性成分的资格,在抗高血糖药物配方和功能食品中的抗氧化剂中具有潜在的应用前景。
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引用次数: 1
MAP kinase and plant–pathogen interactions govern male Zizania latifolia responses to Ustilago esculenta during the early stages of infection MAP激酶和植物-病原体的相互作用决定了在感染的早期阶段,宽叶雄藻对黑罗非鱼的反应
IF 2 4区 农林科学 Q2 HORTICULTURE Pub Date : 2023-04-18 DOI: 10.2478/fhort-2023-0011
Pengfei Guo, Huimin Zhou, LongFei Bai, Yayu Lin, Yalong Zhang, Bichen Wang, Xiaomei He, D. Gan
ABSTRACT This study explored the effect of Ustilago esculenta infection on differentially expressed genes (DEGs) in male Manchurian wild rice (Zizania latifolia). We injected male Jiaobai plants at the five-leaf stage with U. esculenta suspension, and leaves at different timepoints after inoculation were collected for transcriptome sequencing. Transcriptome sequencing identified 1,226 DEGs mainly enriched in resistance-related processes. Weighted gene coexpression network analysis (WGCNA) showed that ‘plant–pathogen interaction’ and ‘MAPK signalling pathway-plant’ were the two most important upregulated pathways, and 16 candidate genes related to response to U. esculenta infection were screened. The results provide a basis for determining the molecular mechanisms of the response of male Z. latifolia to U. esculenta infection.
摘要本研究探讨了乌丝罗非鱼感染对东北野生稻差异表达基因(DEGs)的影响。我们给处于五叶期的雄性胶白植物注射钩藤悬浮液,并收集接种后不同时间点的叶片进行转录组测序。转录组测序鉴定出1226个DEG,主要富集在抗性相关过程中。加权基因共表达网络分析(WGCNA)表明,“植物-病原体相互作用”和“MAPK信号通路-植物”是两个最重要的上调途径,筛选出16个与对钩藤感染反应相关的候选基因。研究结果为确定宽叶泽兰雄性对钩藤感染反应的分子机制提供了依据。
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引用次数: 1
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Folia Horticulturae
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