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First characterisation of chrysanthemum virus B infecting chrysanthemum in Thailand and development of colourimetric RT-LAMP for rapid and sensitive detection 泰国菊花感染病毒B的首次鉴定及快速灵敏检测的比色RT-LAMP的研制
IF 2 4区 农林科学 Q2 Agricultural and Biological Sciences Pub Date : 2022-08-19 DOI: 10.2478/fhort-2022-0012
Salit Supakitthanakorn, T. Mochizuki, Kanjana Vichittragoontavorn, K. Kunasakdakul, Pilunthana Thapanapongworakul, O. Ruangwong
Abstract Chrysanthemum is among the world's most important ornamental plants because of its high economic and cultural value. Our report is the first to describe the detection of chrysanthemum virus B (CVB) in chrysanthemum leaf samples collected from Thailand, which showed yellowing and mild mottling symptoms. The coat protein sequences of CVB isolated in this study share 95.15% identity with previously characterised CVB isolates. Biological indexing found that CVB induced both local and systemic symptoms in tobacco plants, while petunia displayed systemic symptoms. To improve the rapidity and sensitivity of CVB detection, the loop-mediated isothermal amplification (LAMP) technique was developed. LAMP detection was found to be optimal when incubation was conducted at 65 °C for 45 min, wherein the LAMP reaction demonstrated 106 times higher sensitivity than polymerase chain reaction. To simplify the interpretation of results, we designed the method such that a positive result is clearly indicated based on a change of colour (colourimetry), from pink to yellow, as observed visually and via gel electrophoresis. To our best knowledge, this is the first report on the characterisation of molecular, biological and morphological characteristics of CVB infecting chrysanthemum in Thailand, along with the development of colourimetric RT-LAMP for improving detection efficiency.
摘要菊花具有很高的经济和文化价值,是世界上最重要的观赏植物之一。本报告首次报道了从泰国采集的菊花叶片样品中检测到的菊花病毒B (CVB),其表现为泛黄和轻度斑驳症状。本研究分离得到的CVB外壳蛋白序列与先前鉴定的CVB分离株具有95.15%的同源性。生物学指标显示,CVB在烟草植株中引起了局部性和全身性症状,而矮牵牛表现出全身性症状。为了提高CVB检测的快速和灵敏度,建立了环介导等温扩增(LAMP)技术。在65℃孵育45 min时LAMP检测效果最佳,LAMP反应的灵敏度比聚合酶链反应高106倍。为了简化对结果的解释,我们设计了这样一种方法,即根据颜色的变化(比色法),从粉红色到黄色,通过视觉和凝胶电泳观察,明确表示阳性结果。据我们所知,这是泰国首次报道了CVB侵染菊花的分子、生物学和形态学特征,并开发了比色RT-LAMP,以提高检测效率。
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引用次数: 0
Optimization of solvent-free microwave-assisted extraction of antioxidant compounds from Lagenaria siceraria fruit by response surface methodology 响应面法优化无溶剂微波辅助提取西瓜果实抗氧化化合物
IF 2 4区 农林科学 Q2 Agricultural and Biological Sciences Pub Date : 2022-08-19 DOI: 10.2478/fhort-2022-0013
Sahar Iftikhar, Muhammad Tariq Qamar, A. Y. Aydar, D. Ahmed
Abstract Lagenaria siceraria (bottle gourd) fruit is highly celebrated for its nutritional as well as therapeutic properties. The present study was undertaken with the aim of exploring an efficient green solvent-free microwave-assisted extraction method for bioactives from this fruit. Optimization was done according to response surface methodology (RSM), where microwave power (W) and time (s) were independent factors, and percent extraction yield, total phenolic content (TPC), total flavonoid content (TFC), 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity, ferric reducing antioxidant potential (FRAP) and iron chelating activity (ICA) were the responses. TPC, TFC, DPPH radical scavenging activity, FRAP and ICA were highest at 480 W and 60 s. The TPC was 288.9 mg GAE · g−1 DW (milligram gallic acid equivalent per gram dry weight), TFC was 214.1 mg RE · g−1 (rutin equivalent per gram DW), anti-radical activity was 32.96%, FRAP was 289.7 mg AAE · g−1 (mg ascorbic acid equivalents per gram) and ICA was 19.52%. The results of the study thus demonstrate that the solvent-free microwave-assisted extraction method, which utilised an optimum power of 480 W and a time of 60 s, is an effective and green method for extraction of antioxidant compounds from bottle gourd fruit.
摘要冬瓜(Lagenaria siceraria)果实因其营养和治疗特性而备受赞誉。本研究旨在探索一种有效的绿色无溶剂微波辅助提取该果实生物活性物质的方法。根据响应面法(RSM)进行优化,其中微波功率(W)和时间(s)是独立因素,提取率、总酚含量(TPC)、总黄酮含量(TFC)、1,1-二苯基-2-苦基肼(DPPH)自由基清除活性、铁还原抗氧化潜力(FRAP)和铁螯合活性(ICA)是响应。TPC、TFC、DPPH自由基清除活性、FRAP和ICA在480 W和60 s时最高。TPC为288.9 mg GAE·g−1 DW(毫克没食子酸当量/克干重),TFC为214.1 mg RE·g−2(芦丁当量/克DW),抗自由基活性为32.96%,FRAP为289.7mg AAE·g−1(mg抗坏血酸当量/克),ICA为19.52%。研究结果表明,无溶剂微波辅助提取法是一种从葫芦果实中提取抗氧化化合物的有效且绿色的方法,最佳功率为480W,时间为60s。
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引用次数: 2
Foliar application of potassium silicate, potassium fulvate and betaine improve summer-time tomato yield by promoting plant nitrogen and potassium uptake 叶面施用硅酸钾、黄酸钾和甜菜碱通过促进植株对氮和钾的吸收来提高夏季番茄产量
IF 2 4区 农林科学 Q2 Agricultural and Biological Sciences Pub Date : 2022-08-16 DOI: 10.2478/fhort-2022-0010
Xiangnan Xu, Xihong Lei, Shangqiang Liao, Yanmei Li, Yanxin Sun
Abstract During the summer months, greenhouse tomato production is challenged by the heat, causing yield reduction; therefore, we conducted a study to test the effectiveness of different foliar spray compositions for the improvement of Lycopersicon esculentum var. cerasiforme ‘Qianxi’ nutrition uptake and fruit yield. Two forms of silicon, two kinds of organic nitrogenous compounds and water as the control factor were two-two paired to become nine different recipes, which were as follows: CK (H2O), ISi (K2SiO3), organic silicon (OSi), potassium fulvate (BSFA), BSFA + ISi, BSFA + OSi, betaine (GB), GB + ISi and GB + OSi. The plants were sprayed three times during the period of the first, second and third truss fruit expansions with a 2-week interval. As a result, BSFA or K2SiO3 generated higher yield in plants compared with the other compositions. Also, K2SiO3 significantly enhanced the total nitrogen, phosphorus and potassium accumulation in fruit and the whole plant. Comparing across the nine recipes, BSFA + ISi, ISi and GB had improved the fruit yield by 17%, 12.7% and 9.5%, performing the best. BSFA + ISi, ISi and GB also improved the plant nitrogen uptake by 8.2%, 18.8% and 9.8%, as well as the potassium uptake by 16.2%, 12.3% and 15.2%, compared with CK, respectively. Thus, K2SiO3, BSFA and GB stimulated the plant nitrogen and potassium uptake, which improved the marketable yield.
摘要在夏季,温室番茄生产受到高温的挑战,导致产量下降;因此,我们进行了一项研究,以测试不同的叶面喷雾成分对改善黔西番茄营养吸收和果实产量的有效性。以两种形态的硅、两种有机含氮化合物和水为控制因子,将其两两配对,形成9种不同的配方,分别为:CK(H2O)、ISi(K2SiO3)、有机硅(OSi)、黄酸钾(BSFA)、BSFA+ISi、BSFA+OSi、甜菜碱(GB)、GB+ISi和GB+OSi。在第一次、第二次和第三次特拉斯果实膨胀期间,以2周的间隔对植物进行三次喷雾。结果,与其他组合物相比,BSFA或K2SiO3在植物中产生更高的产量。K2SiO3显著提高了果实和全株总氮、磷、钾的积累。在9个配方中,BSFA+ISi、ISi和GB的果实产量分别提高了17%、12.7%和9.5%,表现最好。与对照相比,BSFA+ISi、ISi和GB也分别提高了植物对氮的吸收8.2%、18.8%和9.8%,对钾的吸收16.2%、12.3%和15.2%。因此,K2SiO3、BSFA和GB刺激了植物对氮和钾的吸收,提高了商品产量。
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引用次数: 4
Mathematical modelling of Hass avocado firmness by using destructive and non-destructive devices at different maturity stages and under two storage conditions 在不同成熟阶段和两种储存条件下,使用破坏性和非破坏性装置对哈斯鳄梨硬度进行数学建模
IF 2 4区 农林科学 Q2 Agricultural and Biological Sciences Pub Date : 2022-08-05 DOI: 10.2478/fhort-2022-0011
V. Uarrota, R. Pedreschi
Abstract Devices which are used to determine firmness of non-destructive nature do not penetrate the skin or damage the underlying flesh of the fruit and do provide real-time results, avoid raw biomass loss and allow the testing of every individual fruit and repeated testing of the same fruit, thus providing many advantages for researchers and the industry alike. Contrarily, destructive methods puncture the fruit and thus only a subsample, selected to represent all fruit, can be tested. In addition, different operators can generate quite different values for the same fruit using a hand-held penetrometer. Hass avocado from two commercial orchards was collected, and fruit firmness was measured at harvest, during two storage conditions controlled atmosphere (CA) and regular air (RA), during shelf-life and at the ready-to-eat stage using both destructive (Fruit Pressure Tester, mod. FT327, Wagner Instruments, Greenwich, USA) and non-destructive devices (Model TA.XT plusC, Stable Micro Systems Ltd, England). Then, the mathematical relationship between these two devices was assessed using Spearman Correlation coefficient (rho) and p-values adjusted by Benjamini-Hochberg and Bonferroni procedures. Thus, 3,200 fruits were evaluated during two harvests and two subsequent seasons. A moderate and positive association between destructive and non-destructive devices was found (rho coefficient ranging from 0.41 to 0.51). The variance explained by the regression models ranged from 0.53 to 0.63; all of them were significant with good accuracies (i.e., 0.79; 0.78; 0.73; 0.76). The results prompt us to conclude that a non-destructive texture analyser device can be used to accurately predict firmness measured by a penetrometer in Hass avocado fruit and contribute to avoiding fruit discards.
用于确定非破坏性性质的硬度的设备不会穿透皮肤或损坏水果的底层果肉,并且提供实时结果,避免原始生物量损失,并允许对每个水果进行测试和重复测试,从而为研究人员和行业提供了许多优势。相反,破坏性方法刺穿水果,因此只有一个子样本,选择代表所有水果,可以测试。此外,不同的操作人员可以使用手持式穿透仪对同一种水果产生完全不同的值。采集了两个商业果园的哈斯鳄梨,并在收获时、受控大气(CA)和常规空气(RA)两种储存条件下、保质期内和即食阶段使用破坏性(fruit Pressure Tester, mod. FT327, Wagner Instruments, Greenwich, USA)和非破坏性装置(Model TA)测量了果实的硬度。XT plusC, Stable Micro Systems Ltd,英国)。然后,使用Spearman相关系数(rho)和benjamin - hochberg和Bonferroni程序调整的p值来评估这两个设备之间的数学关系。因此,在两次收获和随后的两个季节对3,200种水果进行了评估。破坏性和非破坏性装置之间存在适度的正相关(rho系数范围为0.41至0.51)。回归模型解释的方差范围为0.53 ~ 0.63;它们都是显著的,具有良好的准确性(即0.79;0.78;0.73;0.76)。结果提示我们得出结论,非破坏性质地分析仪装置可以准确地预测哈斯鳄梨果实的硬度,并有助于避免水果丢弃。
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引用次数: 2
Zn2+ induces changes in activities of mitochondrial respiratory chain complexes and emissions of floral volatiles in Dendrobium huoshanense Zn2+诱导霍山石斛线粒体呼吸链复合物活性和花挥发物排放的变化
IF 2 4区 农林科学 Q2 Agricultural and Biological Sciences Pub Date : 2022-06-01 DOI: 10.2478/fhort-2022-0009
Wangsheng Zhu, Jun Dai, Jiahong Wang
Abstract In recent years, there has been increasing interest in floral volatiles due to their important function in reproduction, self-protection and so on. Although some progress was made on deciphering emissions of floral volatiles and on related mechanisms under a variety of environmental factors, the effects of Zn2+ concentrations on mitochondrial function and floral volatile emissions are yet to be revealed. Dendrobium huoshanense petals were used as materials and were treated with a 0–8 mM ZnSO4 solution. Intracellular Zn2+ concentrations were evaluated by the fluorescent dye method and atomic absorption method. Mitochondrial respiratory chain complex activities and the precursor and ATP contents were determined by the biochemical method. Floral volatile components were analyzed by the gas chromatograph-mass spectrometer (GC-MS). The results indicated that ZnSO4 application significantly increased intracellular Zn2+ concentrations. Elevated intracellular Zn2+ concentrations differently affected mitochondrial respiratory chain complex activities, precursor and adenosine triphosphate (ATP) productions and floral volatile emissions. Moreover, positive correlations exist among the activities of mitochondrial respiratory chain complexes, productions of precursors and ATP and emissions of floral volatiles. It is concluded that Zn2+ concentrations induce the activity changes of mitochondrial respiratory chain complexes, especially complex II and V, which promote or inhibit the emissions of floral volatiles by affecting the precursor and ATP levels that are closely related to the production of terpenoids, benzoids and fatty acid derivatives. The research will contribute to understanding the relationship between Zn2+ concentrations and floral volatile emissions from the perspective of mitochondrial function.
摘要近年来,植物挥发物因其在繁殖、自我保护等方面的重要功能而受到越来越多的关注。虽然在各种环境因素下对花挥发物排放的解析和相关机制方面取得了一些进展,但Zn2+浓度对线粒体功能和花挥发物排放的影响尚未揭示。以霍山石斛花瓣为材料,用0 ~ 8 mM的ZnSO4溶液处理。采用荧光染料法和原子吸收法测定细胞内Zn2+浓度。采用生化法测定线粒体呼吸链复合体活性、前体及ATP含量。采用气相色谱-质谱联用仪(GC-MS)分析了花的挥发性成分。结果表明,施用ZnSO4显著提高了细胞内Zn2+浓度。细胞内Zn2+浓度升高对线粒体呼吸链复合体活性、前体和三磷酸腺苷(ATP)的产生以及花挥发物排放有不同程度的影响。此外,线粒体呼吸链复合物的活性、前体和ATP的产生与花挥发物的排放呈正相关。由此可见,Zn2+浓度可诱导线粒体呼吸链复合物,尤其是复合物II和V的活性变化,通过影响与萜类、苯甲酸和脂肪酸衍生物产生密切相关的前体和ATP水平,促进或抑制花挥发物的排放。该研究将有助于从线粒体功能的角度了解Zn2+浓度与花挥发物排放的关系。
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引用次数: 1
Perceived functions of allotment gardens and their importance during the COVID-19 pandemic in Poland 分配花园的感知功能及其在波兰COVID-19大流行期间的重要性
IF 2 4区 农林科学 Q2 Agricultural and Biological Sciences Pub Date : 2022-05-13 DOI: 10.2478/fhort-2022-0006
Edyta Janus, B. Szewczyk-Taranek, A. Smrokowska-Reichmann
Abstract This article describes the changes taking place in the functions of allotment gardens, their perceived value, reasons for purchasing allotments and subjective (self-reported) assessments of their importance during the pandemic. A questionnaire survey was conducted among 203 owners of allotment gardens located in three highly urbanised cities in the Silesian Voivodeship (Southern Poland). Semi-structured questionnaires and non-standardised questionnaires were used to collect the data. The results demonstrate that the respondents aged above 61 years (38.5%) have observed a generational change in the function of allotments, from cultivating fruits and vegetables to recreational purposes. Regardless of age, the owners of allotment gardens did not notice any technological progress or new crops. The young respondents (21–30 years) treated allotment gardens as an investment (36.7%), while the respondents aged below 20 years and over 61 years declared that the greatest benefits of allotment gardening are improved health and growing one’s own fruits and vegetables. The respondents aged over 41 years (25.9%) also pointed out the importance of growing their own produce. Allotments were especially important during the pandemic as a private space free from COVID-related restrictions. Extended interviews with the respondents revealed that allotment gardening was perceived as a coping strategy for the stress generated during the lockdown. This study showed that allotment gardens are important sites not only for food production but also for maintaining mental health, social capital and environmental engagement.
摘要本文描述了分配花园功能的变化、其感知价值、购买分配的原因以及对其重要性的主观(自我报告)评估。对西里西亚省(波兰南部)三个高度城市化城市的203名分配花园业主进行了问卷调查。采用半结构化问卷和非标准化问卷进行数据收集。结果表明,61岁以上的受访者(38.5%)观察到分配功能的代际变化,从种植水果和蔬菜到娱乐目的。无论年龄大小,分配花园的所有者都没有注意到任何技术进步或新作物。年轻受访者(21-30岁)将分配花园视为一种投资(36.7%),而年龄在20岁以下和61岁以上的受访者则表示,分配花园的最大好处是改善健康和种植自己的水果和蔬菜。年龄在41岁以上的受访者(25.9%)也指出了种植自己的农产品的重要性。在疫情期间,作为一个没有新冠肺炎相关限制的私人空间,分配尤为重要。对受访者的长期采访显示,分配园艺被视为应对封锁期间产生的压力的一种策略。这项研究表明,分配花园不仅是粮食生产的重要场所,也是保持心理健康、社会资本和环境参与的重要场所。
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引用次数: 3
Therapeutic horticulture as a potential tool of preventive geriatric medicine improving health, well-being and life quality – A systematic review 治疗性园艺作为预防性老年医学的潜在工具,改善健康、福祉和生活质量——系统综述
IF 2 4区 农林科学 Q2 Agricultural and Biological Sciences Pub Date : 2022-05-13 DOI: 10.2478/fhort-2022-0008
Anna Heród, B. Szewczyk-Taranek, B. Pawłowska
Abstract The global population of older people grows systematically and with age, the physical and cognitive abilities of people decline. The amount of evidence that gardening may provide substantial health benefits and enhance the quality of ageing is increasing. This paper presents a systematic review of the therapeutic effects of horticulture and gardening on clients aged ≥60 years. It encompasses articles published in English between January 1, 2010, and December 31, 2021. The literature survey shows that the interest in the topic has grown significantly in recent years as over half of the published studies are from 2019 to 2021. Most of this work was done in Asia (60%), America, Europe, Australia and New Zealand. The most commonly used interventions were active horticultural therapy programs or gardening, but 20% of the studies explored the passive connection of being outside. The fitness of the elderly was measured using 33 psychological tests, 32 physiological and functional parameters and different kinds of self-developed questionnaires and interviews. The most commonly used psychological tests were the Geriatric Depression Scale, Self-rated Health and Quality of Life, Mini-Mental State Examination, Friendship Scale, Lubben Social Network Scale, and the Attitudes to Ageing Questionnaire. The physiological and functional parameters included heart rate variability, blood pressure, electroencephalography, brain nerve growth factors, and different types of biomarkers. The study outcomes demonstrated positive results of horticultural therapy on human health and well-being, particularly in a psychological dimension and to a smaller but still significant extent physiological aspect.
全球老年人口系统增长,随着年龄的增长,人们的身体和认知能力下降。越来越多的证据表明,园艺可以提供实质性的健康益处,并提高老龄化的质量。本文系统回顾了园艺和园艺对年龄≥60岁的客户的治疗效果。它涵盖了2010年1月1日至2021年12月31日期间发表的英文文章。文献调查显示,近年来对该主题的兴趣显著增长,因为超过一半的已发表研究是在2019年至2021年之间。其中大部分工作是在亚洲(60%)、美洲、欧洲、澳大利亚和新西兰完成的。最常用的干预措施是积极的园艺治疗项目或园艺,但20%的研究探索了户外活动的被动联系。采用33项心理测试、32项生理和功能参数以及自行编制的各种问卷和访谈对老年人的健康状况进行了测量。最常用的心理测试是老年抑郁量表、自评健康与生活质量量表、简易心理状态量表、友谊量表、卢本社会网络量表和对衰老的态度问卷。生理和功能参数包括心率变异性、血压、脑电图、脑神经生长因子和不同类型的生物标志物。研究结果表明,园艺疗法对人类健康和福祉产生了积极的影响,特别是在心理方面,以及在较小但仍很重要的生理方面。
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引用次数: 1
Comparative RNA-Seq analysis to understand anthocyanin biosynthesis and regulations in Curcuma alismatifolia 比较RNA-Seq分析以了解泽泻花青素的生物合成和调控
IF 2 4区 农林科学 Q2 Agricultural and Biological Sciences Pub Date : 2022-05-08 DOI: 10.2478/fhort-2022-0007
Yuan-Yuan Li, Xiao-Huang Chen, Huiyang Yu, Qilin Tian, Luanmei Lu
Abstract Anthocyanins play a critical role in flower colour pattern formation, and their biosynthesis is typically regulated by transcription factors (TFs). Curcuma alismatifolia is a well-known ornamental plant with colourful flowers. However, little is known about the genes that regulate anthocyanin accumulation in C. alismatifolia. In the present study, high-quality RNA was extracted from three flowering stages of ‘Dutch Red’ and the blossoming stage of ‘Chocolate’. In all, 576.45 Mb clean data and 159,687 de-redundant sequences were captured. The Kyoto Encyclopedia of Genes and Genomes analysis showed that the pathways of phenylpropanoid biosynthesis, flavonoid biosynthesis, flavone and flavonol biosynthesis, and terpenoid backbone biosynthesis were the most enriched. Thirty unique isoforms were annotated as encoding enzymes or TFs involved in anthocyanin biosynthesis. Further analysis showed that the up-regulation of anthocyanin biosynthesis genes was associated with the red colour formation of ‘Dutch Red’, and their expression was induced at the initial flowering stage. The gene flavonoid 3′, 5′-hydroxylase, a key enzyme in the formation of delphinidin-based anthocyanins, reduced expression in ‘Chocolate’. In addition, we identified totally 14 TFs including 11 MYB proteins and 3 WD proteins, which might play important roles in the regulation of anthocyanin biosynthesis. The quantitative Real-Time Polymerase Chain Reaction (qRT-PCR) results were generally consistent with the high-throughput sequencing results. Together, the results of our study provide a valuable resource for the regulatory mechanism of anthocyanin biosynthesis in C. alismatifolia and for the breeding of Curcuma cultivars with novel and charming flower colours.
摘要花青素在花色图案的形成中起着至关重要的作用,其生物合成通常受转录因子(transcription factors, TFs)调控。姜黄是一种著名的观赏植物,花色鲜艳。然而,调控茜草花青素积累的基因却知之甚少。在本研究中,从“荷兰红”的三个开花阶段和“巧克力”的开花阶段提取了高质量的RNA。总共捕获了576.45 Mb干净数据和159,687个去冗余序列。京都基因与基因组百科分析显示,苯丙类生物合成途径、类黄酮生物合成途径、黄酮和黄酮醇生物合成途径、萜类主链生物合成途径富集程度最高。30种独特的异构体被标注为编码参与花青素生物合成的酶或tf。进一步分析表明,花青素生物合成基因的上调与‘荷兰红’的红色形成有关,其表达是在开花初期诱导的。基因类黄酮3′,5′-羟化酶是形成飞燕草苷基花青素的关键酶,在“巧克力”中表达减少。此外,我们共鉴定出14个tf,其中MYB蛋白11个,WD蛋白3个,可能在花青素的生物合成中发挥重要作用。定量实时聚合酶链反应(qRT-PCR)结果与高通量测序结果基本一致。本研究结果为进一步研究姜黄花青素合成的调控机制和培育花色新颖的姜黄品种提供了宝贵的资源。
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引用次数: 1
Screening of composite substrates suitable for tissue-cultured plantlets growth of Asparagus cochinchinensis (Lour.) Merr 适合南美白芦笋组织培养苗生长的复合基质的筛选
IF 2 4区 农林科学 Q2 Agricultural and Biological Sciences Pub Date : 2022-03-07 DOI: 10.2478/fhort-2022-0004
Xiaosong Yu, Lin Li, Yongquan Li, Z. Liu, N. Yang, Ming-sheng Zhang
Abstract Asparagus cochinchinensis (Lour.) Merr is a medicinal, edible and horticulture plant. Seedling breeding relies on tissue-cultured technology, but the transplant from tissue-cultured seedlings to field cultivation poses a problem in the large-scale production of A. cochinchinensis. Tissue-cultured seedlings of A. cochinchinensis were used as materials, and they were transplanted into the substrates that were mixed with coconut bran, peat, vermiculite, perlite or garden soil. The analysis of the physical and chemical properties of the substrate, combined with the survival rate of seedlings, the aboveground parameters (plant height, stem diameter, fresh weight and dry weight), underground parameters (root fresh weight, root dry weight, root length, root diameter, root surface area and root volume) and physiological indexes (malondialdehyde, soluble protein, soluble sugar, proline, chlorophyll and root vigour), allow us to understand the effects of different combination substrates on the growth and physiology of A. cochinchinensis tissue-cultured seedlings. We concluded that the survival rate of seedlings was negatively correlated with the bulk density and conductivity and was positively correlated with the porosity in the substrate. The membership function was used to comprehensively evaluate the indexes of each combination substrate, and it was concluded that coconut bran:perlite:vermiculite = 33.33%:33.33%:33.33% (bulk density = 0.23 g · cm−3, total porosity = 65.07%, pH = 6.30, conductivity = 0.51 mS · cm−1) scored the highest, the survival rate of seedlings reached 87.10%, the aboveground and underground growth were vigorous and the quality was better than other substrates. This research provides technical support for the efficient cultivation of tissue-cultured seedlings of A. cochinchinensis and reduces the cost of raising seedlings.
摘要芦笋是一种药用、食用和园艺植物。育苗依赖于组织培养技术,但从组织培养的幼苗移植到田间栽培,给胭脂虫的大规模生产带来了问题。以胭脂虫的组织培养苗为材料,将其移植到与椰子皮、泥炭、蛭石、珍珠岩或花园土壤混合的基质中。基质的理化性质分析,结合幼苗的存活率、地上参数(株高、茎径、鲜重和干重),地下参数(根鲜重、根干重、根长、根径、根表面积和根体积)和生理指标(丙二醛、可溶性蛋白、可溶性糖、脯氨酸、叶绿素和根系活力),使我们能够了解不同组合基质对胭脂虫组织培养苗生长和生理的影响。我们得出的结论是,幼苗的存活率与体积密度和电导率呈负相关,与基质中的孔隙率呈正相关。利用隶属函数对各组合基质的各项指标进行综合评价,得出椰子糠:珍珠岩:蛭石=33.33%:33.33%(容重=0.23 g·cm−3,总孔隙率=65.07%,pH=6.30,电导率=0.51 mS·cm−1)得分最高,幼苗成活率达87.10%,地上和地下生长旺盛,品质优于其他基质。本研究为高效培育胭脂虫组织培养苗提供了技术支持,降低了育苗成本。
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引用次数: 1
Differences in health-promoting properties in civilisation diseases of Agaricus bisporus fruiting bodies harvested from three flushes 三次冲洗双孢蘑菇子实体在文明疾病中促进健康特性的差异
IF 2 4区 农林科学 Q2 Agricultural and Biological Sciences Pub Date : 2022-02-27 DOI: 10.2478/fhort-2022-0002
A. Włodarczyk, A. Krakowska, J. Lazur, K. Sułkowska-Ziaja, B. Muszyńska
Abstract A controlled environment and composition of the compost used for the commercial production of Agaricus bisporus can allow fruiting bodies with increased nutritional value to be obtained. The present study aimed first to investigate the content of bioelements in fruiting bodies of A. bisporus harvested from three flushes of cultivation and to estimate which elements are accumulated most effectively by determination of bioconcentration factor (BCF). The second aim of the study is to determine the amount of selected organic compounds in the A. bisporus fruiting bodies harvested from three flushes of cultivation and to evaluate their antioxidant activity. Among the three examined A. bisporus flushes, the first is considered the most valuable, since it was rich in dietary and nutritional substances, and has shown promise of antioxidant and antidepressant activities. The present study is the first to describe a complex evaluation of the content of bioactive substances and antioxidant activity in three different cultivation flushes of A. bisporus fruiting bodies.
摘要用于双孢蘑菇商业生产的堆肥的控制环境和组成可以使子实体获得更高的营养价值。本研究旨在首先调查从三次培养中收获的双孢A.bisporus子实体中的生物元素含量,并通过测定生物富集因子(BCF)来估计哪些元素积累最有效。该研究的第二个目的是确定从三次冲洗培养中收获的双孢A.bisporus子实体中所选有机化合物的量,并评估其抗氧化活性。在三种检查的双孢蘑菇潮红中,第一种被认为是最有价值的,因为它富含膳食和营养物质,并且显示出抗氧化和抗抑郁活性的前景。本研究首次描述了对双孢霉子实体三种不同培养液中生物活性物质含量和抗氧化活性的复杂评估。
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引用次数: 0
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Folia Horticulturae
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