Food Bioscience最新文献_第2页

Lacticaseibacillus rhamnosus FJG1530 modulate gut microbiota and liver metabolism to alleviate lipopolysaccharide-induced acute liver injury in mice 鼠李糖乳杆菌FJG1530调节肠道菌群和肝脏代谢，减轻脂多糖诱导的小鼠急性肝损伤

IF 5.9 1区农林科学 Q1 FOOD SCIENCE & TECHNOLOGY

Food Bioscience

Pub Date : 2026-01-23 DOI: 10.1016/j.fbio.2026.108348

Siyu He , Weiling Guo , Yuheng Yang , Li Ni , Xucong Lv , Youting Chen

Acute liver injury (ALI) is one of the most prevalent liver diseases, which have become a major health threat worldwide. Here, the meaning of this research is to explore the ability of L. rhamnosus FJG1530 to alleviate ALI in mice. In this study, L. rhamnosus FJG1530 alleviated lipopolysaccharide (LPS)-induced ALI symptoms, including elevated transaminase (aspartate aminotransferase [AST], and alanine aminotransferase [ALT]) levels, liver tissue damage, increased liver oxidative stress (superoxide dismutase [SOD], catalase [CAT], glutathione [GSH], and total antioxidant capacity [T-AOC]), and high concentration of inflammatory cytokines (tumor necrosis factor-α [TNF-α], interleukin-1β [IL-1β], and interleukin-6 [IL-6]). Additionally, the mice treated with L. rhamnosus FJG1530 showed an upward trend in short-chain fatty acid content in the cecum, and alteration of the gut microbiota structure. The study also discovered that L. rhamnosus FJG1530 intervention improved the composition of liver metabolites, and these characteristic biomarkers mainly involving galactose metabolism, histidine metabolism, ascorbate and aldarate metabolism etc. In summary, L. rhamnosus FJG1530 has the ability to prevent the occurrence of ALI through impairing the inflammatory response, curbing liver oxidative stress, and adjusting the gut microbiota structure.

急性肝损伤（ALI）是最常见的肝脏疾病之一，已成为世界范围内主要的健康威胁。本研究的意义在于探讨鼠李糖FJG1530对小鼠ALI的缓解作用。在本研究中，鼠李糖FJG1530缓解了脂多糖（LPS）诱导的ALI症状，包括转氨酶（天冬氨酸转氨酶[AST]、丙氨酸转氨酶[ALT]）水平升高、肝组织损伤、肝脏氧化应激（超氧化物歧化酶[SOD]、过氧化氢酶[CAT]、谷胱甘肽[GSH]、总抗氧化能力[T-AOC]）升高、炎症细胞因子（肿瘤坏死因子-α [TNF-α]、白细胞介素-1β [IL-1β]、白细胞介素-6 [IL-6]）浓度升高。此外，鼠李糖乳杆菌FJG1530处理小鼠的盲肠短链脂肪酸含量呈上升趋势，肠道菌群结构发生改变。本研究还发现鼠李糖FJG1530干预改善了肝脏代谢产物的组成，这些特征性生物标志物主要包括半乳糖代谢、组氨酸代谢、抗坏血酸和醛酸盐代谢等。综上所述，鼠李糖FJG1530具有通过损害炎症反应、抑制肝脏氧化应激、调节肠道菌群结构来预防ALI发生的能力。

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引用次数: 0

Deciphering poultry microbial ecosystems by classical and modern tools 通过经典和现代工具破译家禽微生物生态系统

IF 5.9 1区农林科学 Q1 FOOD SCIENCE & TECHNOLOGY

Food Bioscience

Pub Date : 2026-01-22 DOI: 10.1016/j.fbio.2026.108356

Natalia Merino , Laura Espina , Daniel Berdejo , Rafael Pagán , Diego García-Gonzalo

Food surveillance programs have traditionally relied on culture-dependent tools for the detection and enumeration of microbial groups along the food chain. While essential, these approaches provide a limited view of complex microbial ecosystems, often underestimating fastidious and viable but non-culturable microorganisms. In recent years, culture-independent tools, including sequencing and omics-based strategies, offer complementary insights into microbial diversity and function. Given the global consumption of poultry meat and its significance for food safety, spoilage, and antimicrobial resistance dissemination, a comprehensive characterization of poultry-associated microbial communities is essential. This review critically examines culture-dependent and culture-independent approaches to study the microbiome, resistome, virulome, and mobilome across the poultry production chain, comparing the type of information generated, their advantages and limitations. Culture-dependent methods enable quantification and isolation of viable strains, while culture-independent approaches reveal microbial diversity and functional genes related to antimicrobial resistance, virulence, and genetic mobility. Integrating both strategies strengthens surveillance, improves risk assessment, and supports targeted interventions throughout the poultry sector. This review also highlights key priorities for future research, including greater attention to post-slaughter processing environments, a more systematic investigation of the mobilome and virulome, and the integration of multi-omics, culturomics, and quasi-metagenomics to better link microbial diversity with functional activity and viability.

食品监测项目传统上依赖于培养依赖的工具来检测和枚举沿着食物链的微生物群。虽然这些方法是必要的，但对复杂的微生物生态系统提供了有限的看法，往往低估了挑剔和有活力但不可培养的微生物。近年来，非培养工具，包括测序和基于组学的策略，提供了对微生物多样性和功能的补充见解。鉴于禽肉的全球消费及其对食品安全、腐败和抗菌素耐药性传播的重要性，对家禽相关微生物群落进行全面表征至关重要。这篇综述严格审查了培养依赖和培养独立的方法来研究家禽生产链上的微生物组、抗性组、病毒组和可移动组，比较了所产生的信息类型、它们的优势和局限性。培养依赖的方法能够量化和分离活菌，而培养独立的方法揭示微生物多样性和与抗菌素耐药性、毒力和遗传流动性相关的功能基因。将这两种战略结合起来可加强监测，改进风险评估，并支持整个家禽业有针对性的干预措施。这篇综述还强调了未来研究的重点，包括更多地关注屠宰后加工环境，更系统地研究移动组和病毒组，以及整合多组学、培养组学和准宏基因组学，以更好地将微生物多样性与功能活性和生存能力联系起来。

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引用次数: 0

Improving Penaeus monodon survival during live transport: Gradient cooling acclimation reduces stress and enhances immunity through proteomic modulation 提高单对虾在活体运输中的存活率：梯度冷却驯化通过蛋白质组调节减少应激和增强免疫力

IF 5.9 1区农林科学 Q1 FOOD SCIENCE & TECHNOLOGY

Food Bioscience

Pub Date : 2026-01-22 DOI: 10.1016/j.fbio.2026.108332

Kun Ren , Mengtong Wu , Tianle Cao , Longteng Zhang , Chuan Li , Xiaoshuan Zhang , Yanfu He

Live transportation of Penaeus monodon is critical for preserving shrimp quality in aquaculture operations, yet conventional transport without dormancy induction often results in severe stress and mortality. However, studies investigating the stress response and regulatory adaptations of P. monodon during transport remain limited. This study examined the impact of low-temperature acclimation on the transport survival of P. monodon, compared to traditional non-cooled (WC) storage. Water quality parameters, physiological, biochemical, and proteomic responses were analyzed. The WC group showed a significant decline in dissolved oxygen (DO) levels and an increase in ammonia nitrogen concentration; hemolymph glucose levels progressively declined, whereas catalase (CAT), alkaline phosphatase (ALP), and superoxide dismutase (SOD) activities initially increased before decreasing sharply; lactate (LA) accumulation was severe, and survival rates dropped to 5 % after 4 h of live transport. In contrast, low-temperature acclimation improved physiological status, with the GC group showing optimal performance, including a 56.5 % reduction in glucose consumption rate during the first 4 h compared to WC; endpoint ALP activity of gradient cooling (GC) was 136.2 % of that in the WC group and 108.5 % of that in the acute cooling (AC) group, respectively; CAT and SOD activities remained stably elevated, whereas the other groups showed significant late-stage declines. Proteomic analysis revealed that the AC group upregulated proteins associated with oxidative phosphorylation, ATP metabolism, stress responses, and apoptosis, while the GC group promoted the expression of glycolysis- and immune-related proteins. GC acclimation improved the transport survival of P. monodon by reducing metabolism, mitigating stress, and enhancing immunity.

在水产养殖作业中，单节对虾的活体运输对于保持对虾质量至关重要，但不进行休眠诱导的传统运输往往导致严重的应激和死亡。然而，关于单胞单胞菌在运输过程中的应激反应和调节适应性的研究仍然有限。这项研究调查了影响低温适应运输p .他们的生存,相比传统型无冷(WC)存储。分析水质参数、生理生化和蛋白质组学反应。WC组溶解氧（DO）水平显著下降，氨氮浓度显著升高；血淋巴葡萄糖水平逐渐下降，过氧化氢酶（CAT）、碱性磷酸酶（ALP）和超氧化物歧化酶（SOD）活性先升高后急剧下降；乳酸积累严重，活体运输4小时后存活率降至5%。相比之下，低温驯化改善了生理状态，GC组表现最佳，包括前4小时葡萄糖消耗率比WC降低56.5%；终点ALP活性梯度冷却（GC）组分别为WC组的136.2%和急性冷却（AC）组的108.5%；CAT和SOD活性保持稳定升高，而其他组则表现出明显的后期下降。蛋白质组学分析显示，AC组上调了与氧化磷酸化、ATP代谢、应激反应和凋亡相关的蛋白，而GC组促进了糖酵解和免疫相关蛋白的表达。GC驯化通过降低代谢、减轻应激和增强免疫力来提高单胞假单胞菌的转运存活率。

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引用次数: 0

A novel antioxidant system in Lactococcus lactis N8 乳酸乳球菌N8中一种新的抗氧化系统

IF 5.9 1区农林科学 Q1 FOOD SCIENCE & TECHNOLOGY

Food Bioscience

Pub Date : 2026-01-22 DOI: 10.1016/j.fbio.2026.108358

Huan Yang , Xian Xu , Fengming Liu , Duodong Wang , Yongping Xin , Mingqiang Qiao

The ability of Lactococcus lactis to grow under moderate oxygen conditions enables its expansion from the food industry to microbial cell factories. While leveraging known antioxidative regulatory mechanisms represents a promising strategy for engineering robust strains under high oxygen conditions, the complete genetic basis of oxygen tolerance in L. lactis remains incompletely understood. Here, we identified and characterized a novel antioxidative system and its corresponding regulatory mechanism in L. lactis N8. Genetic analyses demonstrated that RmaH, a MarR-family transcriptional regulator, is essential for mediating the oxidative stress response. Transcriptomic and qPCR analysis further revealed that RmaH acts as a transcriptional repressor of dps, which encodes a DNA-binding protein from starved cells (Dps). In vitro and in vivo protein-DNA binding assays confirmed that RmaH specifically binds to the coding sequence (CDS) of dps, with DNase I footprinting precisely identifying the binding motif (TGTAAG-12nt-CTTTCA). Finally, functional investigations revealed that under oxygen conditions, RmaH expression was suppressed, leading to upregulated dps expression. Dps thereby confers cellular protection via two distinct mechanisms: physically shielding DNA from hydroxyl radicals and inhibiting the Fenton reaction through its ferroxidase activity. Collectively, our findings elucidated a previously uncharacterized RmaH-Dps regulatory pathway that enhances oxygen tolerance in L. lactis, providing both mechanistic insight and a potential target for engineering industrially robust strains.

乳酸乳球菌在中等氧条件下生长的能力使其从食品工业扩展到微生物细胞工厂。虽然利用已知的抗氧化调节机制是在高氧条件下设计健壮菌株的一种有前途的策略，但乳酸菌耐氧的完整遗传基础仍不完全清楚。本研究鉴定了乳酸菌N8中一个新的抗氧化系统及其调控机制。遗传分析表明，RmaH，一个marr家族转录调节因子，在介导氧化应激反应中是必不可少的。转录组学和qPCR分析进一步揭示RmaH作为dps的转录抑制因子，其编码来自饥饿细胞（dps）的dna结合蛋白。体外和体内蛋白- dna结合实验证实RmaH特异性结合dps的编码序列（CDS）， DNase I足迹精确识别结合基序（TGTAAG-12nt-CTTTCA）。最后，功能研究显示，在氧气条件下，RmaH表达被抑制，导致dps表达上调。因此，Dps通过两种不同的机制提供细胞保护：物理上屏蔽DNA免受羟基自由基的侵害，并通过其铁氧化酶活性抑制芬顿反应。总的来说，我们的研究结果阐明了一个以前未被表征的RmaH-Dps调节途径，该途径增强了乳杆菌的氧耐受性，为工程工业健壮菌株提供了机制见解和潜在目标。

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引用次数: 0

Enhancing odd-chain fatty acid biosynthesis in R. opacus via black bean protein hydrolysate-mediated metabolic reprogramming 通过黑豆蛋白水解物介导的代谢重编程促进黑豆R. opacus奇链脂肪酸生物合成

IF 5.9 1区农林科学 Q1 FOOD SCIENCE & TECHNOLOGY

Food Bioscience

Pub Date : 2026-01-22 DOI: 10.1016/j.fbio.2026.108345

Kun Liu, Zhaojun Zheng, Yuanfa Liu

OCFA production from Rhodococcus opacus PD630 (R. opacus), which is a natural oleaginous bacterium, is limited by propionyl-CoA availability despite its potential for biofuel, nutritional, and pharmaceutical applications. This study investigated the effects of enzymatically hydrolyzed black bean protein hydrolysates (BBPHs) as an alternative nitrogen source to stimulate OCFA biosynthesis. Among six hydrolysates, BBPH hydrolyzed by bromelain, flavourzym and Neutrase® markedly enhanced cell growth and lipid accumulation, achieving a 3-fold increase in OCFA content and elevating OCFA proportion to 30.78 ± 0.97 % of total fatty acids. The OCFA content has reached 1368.98 ± 157.08 mg/L in BBPH-N. Metabolomic and lipidomic analyses revealed that BBPH supplementation significantly increased intracellular propionyl-CoA levels (750.29 ± 45.00 ng/mg, compared with the 476.70 ± 36.71 ng/mg of CN group), and modulated succinyl-CoA abundance, suggesting activation of the methyl malonyl-CoA pathway. A total of 69 OCFA-containing triglycerides and 17 diglycerides were identified, with TG 54:1 (TG 17:0_19:0_18:1) emerging as a common lipid marker across BBPH supplementations. Moreover, BBPH hydrolyzed by Neutrase® enhanced the accumulation of C17-series fatty acids and improved nitrogen assimilation efficiency, as indicated by higher biomass and supernatant protein concentrations. Collectively, these results demonstrate that BBPH can reprogram carbon–nitrogen metabolism in R. opacus, promoting propionyl-CoA-derived OCFA synthesis. This work highlights a green and economically viable approach to microbial lipid production using agro-industrial byproducts, contributing to the advancement of circular bioeconomy strategies.

不透明红球菌PD630 （R. opacus）是一种天然产油细菌，其OCFA的生产受到丙酰辅酶a可用性的限制，尽管它在生物燃料、营养和制药方面具有潜力。本研究研究了酶解黑豆蛋白水解物（BBPHs）作为替代氮源刺激OCFA生物合成的效果。在6种水解产物中，经菠萝蛋白酶、风味酶和Neutrase®水解的BBPH显著促进了细胞生长和脂质积累，OCFA含量增加3倍，OCFA占总脂肪酸的比例达到30.78±0.97%。BBPH-N中OCFA含量达1368.98±157.08 mg/L。代谢组学和脂质组学分析显示，BBPH添加显著增加了细胞内丙酰辅酶a水平（750.29±45.00 ng/mg， CN组为476.70±36.71 ng/mg），并调节了琥珀酰辅酶a丰度，表明甲基丙二酰辅酶a途径被激活。共鉴定出69种含ocfa的甘油三酯和17种双甘油三酯，其中TG 54:1 （TG 17:0_19:0_18:1）是BBPH补充剂中常见的脂质标志物。此外，由Neutrase®水解的BBPH增强了c17系列脂肪酸的积累，提高了氮同化效率，这表明了更高的生物量和上清蛋白浓度。综上所述，这些结果表明BBPH可以重编程R. opacus的碳氮代谢，促进丙酰辅酶a衍生的OCFA合成。这项工作强调了利用农业工业副产品生产微生物油脂的绿色和经济可行的方法，有助于推进循环生物经济战略。

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引用次数: 0

Mechanism of hepatoprotection by sugarcane polyphenols against EtOH-induced injury: multi-target regulation of antioxidant defense and alcohol metabolism 甘蔗多酚抗氧化损伤的保肝机制：抗氧化防御和酒精代谢的多靶点调控

IF 5.9 1区农林科学 Q1 FOOD SCIENCE & TECHNOLOGY

Food Bioscience

Pub Date : 2026-01-22 DOI: 10.1016/j.fbio.2026.108360

Min Wang , Yumei Wang , Jiulong An , Shenghong Yao , Chengfeng Zhang , Yanv Zhou , He Li

To elucidate the multi-target mechanisms of sugarcane polyphenols (SP) against alcohol-induced liver injury, this study integrated cellular models with computational simulations: the HepG2 cell model was utilized to validate their efficacy in modulating antioxidant defense and alcohol metabolism, while molecular docking and dynamics simulations were employed to unravel the interactions between key phenolic constituents and the core target proteins, cytochrome P450 2E1 (CYP2E1) and Kelch-like ECH-associated protein 1 (Keap1). The results demonstrated that SP significantly attenuated ethanol (EtOH)-induced hepatocellular damage, as evidenced by a dose-dependent reduction in the activities of alanine aminotransferase, aspartate aminotransferase, and lactate dehydrogenase. SP activated alcohol dehydrogenase and aldehyde dehydrogenase, supporting the primary pathway of alcohol metabolism. SP also improved antioxidant status, as reflected by increased catalase and glutathione levels, decreased malondialdehyde, and, at the protein level, decreased Keap1 and increased heme oxygenase-1 expression, thereby strengthening antioxidant defenses. Computational simulations identified chlorogenic acid (CA) as the key bioactive constituent, which formed the most stable complexes with both CYP2E1 and Keap1, demonstrating hydrogen bonding with key residues including Thr307 and Gln358 in CYP2E1 and Val604 in Keap1. The other phenolic components exhibited similar interactions, collectively contributing to the overall bioactivity of SP. This work elucidates the multi-target hepatoprotective mechanism of SP, supporting its potential as a functional food ingredient from agricultural by-products for dietary strategies against acute EtOH-induced liver injury.

为了阐明甘蔗多酚（SP）抗酒精性肝损伤的多靶点机制，本研究将细胞模型与计算模拟相结合：利用HepG2细胞模型验证其在调节抗氧化防御和酒精代谢方面的功效，并通过分子对接和动力学模拟揭示关键酚类成分与核心靶蛋白细胞色素P450 2E1 （CYP2E1）和kelch样ECH-associated protein 1 （Keap1）之间的相互作用。结果表明，SP显著减轻乙醇（EtOH）诱导的肝细胞损伤，其证据是丙氨酸转氨酶、天冬氨酸转氨酶和乳酸脱氢酶的活性呈剂量依赖性降低。SP激活醇脱氢酶和醛脱氢酶，支持醇代谢的主要途径。SP还能提高抗氧化能力，表现为过氧化氢酶和谷胱甘肽水平升高，丙二醛水平降低，蛋白水平上Keap1表达降低，血红素氧化酶-1表达升高，从而增强抗氧化能力。计算模拟发现绿原酸（CA）是关键的生物活性成分，它与CYP2E1和Keap1形成最稳定的配合物，与CYP2E1中的Thr307和Gln358以及Keap1中的Val604等关键残基形成氢键。其他酚类成分也表现出类似的相互作用，共同促进了SP的整体生物活性。这项研究阐明了SP的多靶点肝保护机制，支持其作为一种功能性食品成分的潜力，从农业副产品中提取，用于饮食策略，以对抗急性etoh诱导的肝损伤。

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引用次数: 0

Enhanced recovery of fluorescent pigment–protein complexes from Spirulina biomass via ultrasound-assisted deep eutectic solvent extraction: Toward sustainable natural food colorants with anti-colorectal cancer potential 超声辅助深共熔溶剂萃取法提高螺旋藻生物质荧光色素-蛋白复合物的回收率：具有抗结直肠癌潜力的可持续天然食用色素

IF 5.9 1区农林科学 Q1 FOOD SCIENCE & TECHNOLOGY

Food Bioscience

Pub Date : 2026-01-22 DOI: 10.1016/j.fbio.2026.108350

Wageeporn Maneechote , Wasu Pathom-aree , Nakarin Suwannarach , Patcharin Chaijaem , Benjamas Cheirsilp , Supakit Chaipoot , Piroonporn Srimongkol , Shuhao Huo , Sirasit Srinuanpan

Phycocyanin (PC), a blue fluorescent pigment–protein derived from Spirulina, possesses potent antioxidant and anticancer activities, yet large-scale production is limited by low yield and instability. This study developed a sustainable and high-efficiency extraction process using a natural deep eutectic solvent (NADES; glycerol–glucose) integrated with ultrasound-assisted extraction (UAE) and freeze–thaw pretreatment. Response surface methodology (RSM) optimized the process at a glycerol concentration of 2.35 M, biomass-to-solvent ratio of 1:17.6 g/mL, extraction time of 66 min, and temperature of 40 °C. The optimized conditions yielded high-quality food-grade PC (7.56 g/100 g biomass), with purity values (A₆₂₀/A₂₈₀ = 0.81–0.93 after dialysis) approaching the lower threshold of cosmetic-grade classification. Kinetic modeling revealed first-order degradation with maximum half-life at pH 6.0 and low temperature, highlighting its stability under mild conditions. SDS–PAGE analysis confirmed the presence of protein bands corresponding to the α- and β-subunits, exhibiting molecular weights in the range of approximately 15–20 kDa, which is characteristic of PC. The extracted PC exhibited high antioxidant capacities (DPPH = 1.90 mg GAE/g; ABTS = 11.30 mg TE/g; PFRAP = 3.12 mg GAE/g) and potent anti-colorectal cancer activity against HT-29 cells (IC₅₀ = 61.42 μg/mL), inducing G1 arrest and apoptosis. This eco-friendly UAE–NADES strategy significantly enhances PC recovery, purity, and bioactivity while aligning with green chemistry principles. The findings provide a scalable platform for producing high-quality, food-grade PC suitable for use as a natural colorant and functional bioactive ingredient in nutraceutical, pharmaceutical, and cosmetic industries.

藻蓝蛋白（Phycocyanin， PC）是一种从螺旋藻中提取的蓝色荧光色素蛋白，具有较强的抗氧化和抗癌活性，但产量低和不稳定性限制了其大规模生产。本研究开发了一种可持续、高效的提取工艺，采用天然深层共晶溶剂（NADES；甘油-葡萄糖）结合超声辅助提取（UAE）和冻融预处理。响应面法优化条件为：甘油浓度2.35 M，料液比1:17.6 g/mL，提取时间66 min，提取温度40℃。优化条件下得到的优质食品级PC （7.56 g/100 g生物质）的纯度（A620/A280 = 0.81-0.93）接近化妆品级分类的下限。动力学模型显示，在pH 6.0和低温条件下，其一级降解半衰期最长，在温和条件下具有稳定性。SDS-PAGE分析证实存在α-和β-亚基对应的蛋白带，分子量约为15-20 kDa，这是PC的特征。提取的PC具有较高的抗氧化能力（DPPH = 1.90 mg GAE/g, ABTS = 11.30 mg TE/g, PFRAP = 3.12 mg GAE/g），对HT-29细胞具有较强的抗结直肠癌活性（IC50 = 61.42 μg/mL），诱导G1阻滞和凋亡。这种环保的UAE-NADES策略显著提高了PC的回收率、纯度和生物活性，同时符合绿色化学原则。这一发现为生产高质量的食品级PC提供了一个可扩展的平台，适合用作天然着色剂和功能性生物活性成分，用于营养保健、制药和化妆品行业。

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引用次数: 0

Yeast fermented alcoholic fruit beverages: A systematic review 酵母发酵酒精水果饮料：系统综述

IF 5.9 1区农林科学 Q1 FOOD SCIENCE & TECHNOLOGY

Food Bioscience

Pub Date : 2026-01-22 DOI: 10.1016/j.fbio.2026.108351

Wenjia He , Tiantian Dong , Yiwei Zhang , Maaria Kortesniemi , Shuxun Liu , Yuting Ding , Xuxia Zhou , Oskar Laaksonen , Baoru Yang

Yeast-driven alcoholic fermentation is widely applied to process fruits into value-added beverages. Fruit genotypes, yeast strains, and beverage production methods significantly influence the diversity and quality of alcoholic beverages. Traditionally, Saccharomyces cerevisiae is regarded as the most commercially important yeast in the alcoholic beverage production markets, whereas other Saccharomyces and non-Saccharomyces is considered to be either harmful or useless. However, the interest of applying these yeasts in numerous innovative yeast fermented alcoholic fruit-based beverages is growing due to their advantageous flavor attributions. In this review, the strategies for enhancing volatile properties and aroma and flavor complexity of alcoholic beverage fermentation were critically examined with a special emphasis on fruit types and yeast strains, as well as their impacts on the quality of alcoholic beverages. The current challenges and future prospects were also discussed on the development of desirable alcoholic beverage production.

酵母驱动的酒精发酵被广泛应用于将水果加工成增值饮料。水果基因型、酵母菌株和饮料生产方法显著影响酒精饮料的多样性和质量。传统上，酿酒酵母菌被认为是酒精饮料生产市场上最重要的商业酵母，而其他酵母菌和非酵母菌则被认为是有害的或无用的。然而，将这些酵母应用于许多创新酵母发酵酒精水果饮料的兴趣正在增长，由于其有利的风味属性。本文综述了提高酒精饮料发酵挥发性、香气和风味复杂性的策略，特别强调了水果类型和酵母菌株，以及它们对酒精饮料质量的影响。并对我国理想酒精饮料的发展面临的挑战和前景进行了探讨。

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引用次数: 0

Ultra-high pressure assisted extraction improves fermentation characteristics and in vitro anti-adipogenic effect of Bangia fusco-purpurea polysaccharide 超高压辅助提取改善了Bangia fusc -purpurea多糖的发酵特性和体外抗脂肪作用

IF 5.9 1区农林科学 Q1 FOOD SCIENCE & TECHNOLOGY

Food Bioscience

Pub Date : 2026-01-22 DOI: 10.1016/j.fbio.2026.108357

Mingjing Zheng , Huan Ouyang , Luan Zhao , Yanbing Zhu , Zhipeng Li , Hui Ni , Zedong Jiang , Tao Hong

In vitro gut microbiota fermentation characteristics and anti-adipogenic effect of Bangia fusco-purpurea polysaccharide extracted with ultra-high pressure assisted extraction (UBFP) were investigated. Compared with polysaccharide obtained with traditional hot water extraction (BFP), UBFP significantly increased intestinal flora diversity and induced a beneficial shift in the microbial composition, which could suppress the growth of opportunistic pathogens such as Escherichia-Shigella and Streptococcus, while enriching the abundance of beneficial bacteria such as Enterococcus. Both BFP and UBFP increased short-chain fatty acids (SCFAs) production, particularly acetic acid. The total SCFAs content in the UBFP group showed a 1.83-fold greater increase than that in the BFP group relative to the control. Furthermore, UBFP fermented products could better inhibit 3T3-L1 preadipocytes from differentiating into mature adipocytes as well as lowering triglyceride and total cholesterol contents and inhibiting lipid droplet formation. Notably, the reduction of TG content by UBFP fermented products showed 1.07- to 2.87-fold greater efficacy than BFP fermented products (p < 0.05). Western blot analysis revealed that UBFP fermentation products upregulated the pAMPK/AMPK ratio and downregulated PPARγ expression in 3T3-L1 cells, suggesting that the anti-adipogenic effect may be mediated through activation of the AMPK signaling pathway and inhibition of PPARγ expression. Although these bioactivities of UBFP were assessed using in vitro models and requires further validation through in vivo studies, this research nevertheless provided valuable insights into the potential role of UBFP in promoting intestinal health and regulating lipid metabolism.

研究了超高压辅助提取法提取的褐紫菜多糖体外肠道菌群发酵特性及抗脂肪作用。与传统热水浸提法（BFP）获得的多糖相比，UBFP显著增加了肠道菌群多样性，诱导了微生物组成的有益转变，抑制了志贺氏杆菌和链球菌等条件致病菌的生长，同时增加了肠球菌等有益菌的丰度。BFP和UBFP都增加了短链脂肪酸（SCFAs）的产生，尤其是乙酸。与对照组相比，UBFP组的总SCFAs含量增加了1.83倍。此外，UBFP发酵产物能更好地抑制3T3-L1前脂肪细胞向成熟脂肪细胞的分化，降低甘油三酯和总胆固醇含量，抑制脂滴形成。值得注意的是，UBFP发酵产品对TG含量的降低效果比BFP发酵产品高1.07 ~ 2.87倍（p < 0.05）。Western blot分析显示，UBFP发酵产物上调3T3-L1细胞pAMPK/AMPK比值，下调PPARγ表达，提示其抗脂肪作用可能通过激活AMPK信号通路，抑制PPARγ表达介导。虽然UBFP的这些生物活性是通过体外模型评估的，需要通过体内研究进一步验证，但该研究为UBFP在促进肠道健康和调节脂质代谢方面的潜在作用提供了有价值的见解。

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引用次数: 0

Heterologous expression of phospholipase A1 gene in PichiaPink™ and optimization of catalytic conditions for phospholipid ω-3 fatty acid synthesis 磷脂酶A1基因在PichiaPink™中的异源表达及磷脂ω-3脂肪酸合成催化条件的优化

IF 5.9 1区农林科学 Q1 FOOD SCIENCE & TECHNOLOGY

Food Bioscience

Pub Date : 2026-01-21 DOI: 10.1016/j.fbio.2026.108344

Mingwei Liu , Ke Zhao , Shufan Liu , Yaoheng Cui , Qing Kong , Dongxing Yu , Yunxiao Ma

In this study, a highly efficient and stable phospholipase A₁ (PLA₁) gene was cloned from Aspergillus oryzae and heterologously expressed in PichiaPink™. The optimized PLA₁ achieved a maximum enzyme activity of 26.42 ± 0.38 U/mL, designated as P1pink. Through scale-up cultivation in a 50 L fermenter, the PLA₁ activity reached 114.30 ± 1.41 U/mL, representing the highest reported activity of PLA₁ in the Pichia expression system. Using fatty acids from fish oil and phosphatidylcholine (PC) from soybeans as substrates, immobilized PLA₁ catalyzed transesterification to successfully synthesize PC containing ω-3 polyunsaturated fatty acids (PUFAs). Detailed investigations on immobilization were conducted, with 724 resin selected as the carrier; the enzyme activity of PLA₁ more than doubled after immobilization. Through transesterification modification of PC, key reaction parameters were optimized to determine the optimal conditions: a substrate mass ratio of 1:5, enzyme addition of 25 %, reaction temperature of 60 °C, water addition of 0.75 %, and reaction time of 32 h. Under these conditions, the total incorporation rate of docosahexaenoic acid/eicosapentaenoic acid (DHA/EPA) reached 22.77 ± 0.62 %. Additionally, the immobilized PLA₁ exhibited excellent reusability, retaining over 50 % of its initial activity after four repeated uses.

本研究从米曲霉（Aspergillus oryzae）中克隆出高效稳定的磷脂酶A1 （PLA1）基因，并在PichiaPink™中异源表达。优化后的PLA1酶活最高为26.42±0.38 U/mL，命名为P1pink。在50 L发酵罐中放大培养，PLA1活性达到114.30±1.41 U/mL，为毕赤酵母表达体系中报道的最高活性。以鱼油脂肪酸和大豆磷脂酰胆碱（PC）为底物，采用固定化PLA1催化酯交换反应，成功合成了含有ω-3多不饱和脂肪酸（PUFAs）的PC。以724树脂为载体，进行了详细的固定化研究；固定化后PLA1酶活性增加一倍以上。通过对PC进行酯交换改性，对关键反应参数进行优化，确定了最佳反应条件：底物质量比为1:5，酶添加量为25%，反应温度为60℃，水添加量为0.75%，反应时间为32 h。在此条件下，二十二碳六烯酸/二十碳五烯酸（DHA/EPA）的总掺入率达到22.77±0.62%。此外，固定化PLA1表现出优异的可重复使用性，在重复使用四次后保留了超过50%的初始活性。

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引用次数: 0