Food Bioscience最新文献_第5页

Preharvest Gibberellic acid (GA3) treatment improves postharvest apple fruit quality by modulating the GABA shunt and volatile compounds accumulation 采前处理赤霉素（GA3）通过调节GABA分流和挥发性化合物积累改善采后苹果果实品质

IF 5.9 1区农林科学 Q1 FOOD SCIENCE & TECHNOLOGY

Food Bioscience

Pub Date : 2026-03-01 Epub Date: 2026-01-19 DOI: 10.1016/j.fbio.2026.108327

Xiaoyun Mao , Niuniu Song , Yali Li , Zhou Si , Yi Luo , Xinying Zhang , Shuo Wang , Yuduan Ding , Yamei Ren

As a typical climacteric fruit, apple undergo quality deterioration during postharvest storage, leading to reduced economic benefits, especially at room temperature. This study investigated the effects of preharvest gibberellin acid(GA₃) application on the storage quality, γ-aminobutyric acid(GABA) shunt and volatile organic compounds(VOCs) of apples. The results showed that GA₃ treatment effectively inhibited ethylene production rate, maintained ascorbic acid (ASA) and total phenol (TP) content, and increased endogenous levels of GABA and glutamic acid (Glu). Meanwhile, GA₃ enhanced glutamate decarboxylase(GAD) activity and upregulated MdGAD expression, while inhibiting γ-Aminobutyrate transaminase(GABA-T) activity and downregulating MdGABA-T expression. In addition, GA₃-treated fruit retained higher level of VOCs during storage compared to the CK. These findings indicated that preharvest GA₃ application improved apple storage quality by coordinately regulating the GABA shunt and VOCs profiles. This finding reveals a novel regulatory pathway of GA₃ in fruit postharvest quality preservation, provides a theoretical basis for the practical application of preharvest GA₃ treatment in apple production, and offers a feasible technical strategy to mitigate postharvest quality loss and improve economic benifits.

苹果作为一种典型的更年期水果，在采后贮藏过程中会发生品质劣化，导致经济效益下降，尤其是常温贮藏。研究了采前施用赤霉素酸（GA3）对苹果贮藏品质、γ-氨基丁酸（GABA）分流和挥发性有机物（VOCs）的影响。结果表明，GA3处理能有效抑制乙烯生成速率，维持抗坏血酸（ASA）和总酚（TP）含量，提高内源GABA和谷氨酸（Glu）水平。同时，GA3增强谷氨酸脱羧酶（GAD）活性，上调MdGAD表达，抑制γ-氨基丁酸转氨酶（GABA-T）活性，下调MdGABA-T表达。此外，ga3处理的果实在贮藏过程中挥发性有机化合物的含量高于对照处理。上述结果表明，采前施用GA3通过协调调节GABA分流和VOCs分布来改善苹果贮藏质量。这一发现揭示了GA3在果实采后品质保鲜中的新调控途径，为采前GA3处理在苹果生产中的实际应用提供了理论依据，为减轻采后品质损失、提高经济效益提供了可行的技术策略。

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引用次数: 0

Effect of structural characteristics of highland barley β-glucan by various thermal processing methods on intestinal microbial community and fermentative products 不同热处理方式青稞β-葡聚糖结构特征对肠道微生物群落及发酵产物的影响

IF 5.9 1区农林科学 Q1 FOOD SCIENCE & TECHNOLOGY

Food Bioscience

Pub Date : 2026-03-01 Epub Date: 2026-01-31 DOI: 10.1016/j.fbio.2026.108376

Rong Li , Ji Gao , Yiyun Zhang , Zijian Dai , Siqi Li , Tong Wu , Jiaqian Cao , Kefan Wei , Chao Wang , Qun Shen , Yong Xue

Highland barley, which is a cereal grain rich in β-glucan, is commonly subjected to various thermal processing methods prior to consumption. Although the influence of β-glucan on gut microbiota has attracted considerable research interest, how thermal treatments affect its prebiotic function remains unclear. In this study, highland barley flour was processed using four thermal methods: steam heating, stir frying, film baking, and extrusion puffing, followed by the extraction of highland barley β-glucan (HBBG). Results revealed that extrusion-puffed HBBG exhibited the most pronounced prebiotic effects. It significantly increased the overall microbial abundance (p < 0.05) and specifically enhanced the growth of several beneficial bacteria, establishing Lactobacillus as a characteristic genus. Furthermore, the extrusion puffing (EP) group showed the highest production of short-chain fatty acids (SCFAs), along with elevated abundances of pathways associated with carbohydrate and amino acid metabolism. Structurally, extrusion puffing resulted in moderate molecular weight, high β-glycosidic bond retention, microporosity, high water-holding capacity, and low viscosity of HBBG, which collectively improving microbial accessibility. This study provides a theoretical foundation for optimizing thermal processing technologies of highland barley β-glucan and suggests that extrusion-puffed HBBG has potential as a novel prebiotic product.

青稞是一种富含β-葡聚糖的谷物，在食用前通常要经过各种热处理方法。虽然β-葡聚糖对肠道微生物群的影响已经引起了相当大的研究兴趣，但热处理如何影响其益生元功能仍不清楚。本研究采用蒸汽加热、爆炒、膜烘、挤压膨化四种热法对青稞面粉进行加工，提取青稞β-葡聚糖（HBBG）。结果表明，挤压膨化的HBBG表现出最显著的益生元效应。它显著提高了总体微生物丰度（p < 0.05），并特异性地促进了几种有益菌的生长，使乳酸杆菌成为一个特征属。此外，挤压膨化（EP）组的短链脂肪酸（SCFAs）产量最高，碳水化合物和氨基酸代谢相关途径的丰度也有所增加。在结构上，挤压膨化使HBBG的分子量适中，β-糖苷键保持率高，微孔隙率高，保水能力强，粘度低，这些都提高了微生物的可及性。本研究为优化青稞β-葡聚糖的热加工工艺提供了理论基础，说明挤压膨化青稞β-葡聚糖具有作为新型益生元产品的潜力。

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引用次数: 0

Ultrasonic treatment enhances the growth of Chlamydomonas reinhardtii through integrated physiological and transcriptomic responses 超声处理通过综合生理和转录组反应促进莱茵衣藻的生长

IF 5.9 1区农林科学 Q1 FOOD SCIENCE & TECHNOLOGY

Food Bioscience

Pub Date : 2026-03-01 Epub Date: 2026-01-30 DOI: 10.1016/j.fbio.2026.108394

Yuxin Liu, Xiaobo Kan, Changhong Liu, Lei Zheng

Chlamydomonas reinhardtii (C. reinhardtii), an emerging food source, currently lacks an efficient induction method for cultivation, limiting its industrial-scale production. In this study, the effects of ultrasonic treatment on the cultivation of C. reinhardtii through integrated physiological and transcriptomic analyses were investigated. The optimal treatment was 175 W for 5 min, applied during the initial cultivation phase. By the end of cultivation, compared to the control, the ultrasonic-treated group exhibited increases of 24 %, 40 %, 24 %, and 44 % in biomass, protein, carbohydrate, and lipid content, respectively, while chlorophyll levels remained unchanged. Ultrasonic treatment induced a transient rise in intracellular peroxides, which was effectively alleviated by enhanced activities of SOD, POD, and CAT. Consequently, no significant differences were observed in final DCF fluorescence values, MDA, and H₂O₂ levels compared to the control. Transcriptomic analysis revealed that ultrasonic treatment significantly upregulated genes involved in carbohydrate metabolism, protein synthesis, and lipid metabolism. Enrichment analyses further revealed that ultrasonic treatment activated MAP kinase activity and downregulated multiple negative regulators linked to metabolic, biological, and biosynthetic processes. The upregulation of acetyl-CoA synthetase (ACS), isocitrate dehydrogenase (IDH1), and 2-oxoglutarate dehydrogenase E1 component (OGDH) enhanced the acetate-driven TCA cycle, thereby contributing to the increase in biomass and major cellular components. These results demonstrate that ultrasonic treatment represents an efficient and promising strategy for the industrial cultivation of C. reinhardtii.

莱茵衣藻（Chlamydomonas reinhardtii， C. reinhardtii）是一种新兴的食物来源，目前缺乏有效的诱导培养方法，限制了其工业化规模生产。本研究通过综合生理和转录组学分析，研究了超声波处理对莱茵青霉培养的影响。最佳处理为175 W， 5 min，在培养初期施用。在培养结束时，与对照组相比，超声波处理组的生物量、蛋白质、碳水化合物和脂质含量分别增加了24%、40%、24%和44%，而叶绿素水平保持不变。超声处理可引起细胞内过氧化物瞬时升高，而SOD、POD和CAT活性的增强可有效缓解这一现象。因此，与对照组相比，最终DCF荧光值、MDA和H2O2水平无显著差异。转录组学分析显示，超声处理显著上调了参与碳水化合物代谢、蛋白质合成和脂质代谢的基因。富集分析进一步表明，超声波处理激活了MAP激酶活性，下调了与代谢、生物和生物合成过程相关的多种负调节因子。乙酰辅酶a合成酶（ACS）、异柠檬酸脱氢酶（IDH1）和2-氧戊二酸脱氢酶E1组分（OGDH）的上调增强了醋酸盐驱动的TCA循环，从而促进了生物量和主要细胞组分的增加。这些结果表明，超声波处理是一种有效和有前途的工业培养策略。

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引用次数: 0

Elucidating the influence of Lactobacillus plantarum 21802 on nutritional and flavor profiles of peach purees from different flesh-colored peach cultivars 探讨植物乳杆菌21802对不同肉色桃品种桃泥营养和风味的影响

IF 5.9 1区农林科学 Q1 FOOD SCIENCE & TECHNOLOGY

Food Bioscience

Pub Date : 2026-03-01 Epub Date: 2026-01-22 DOI: 10.1016/j.fbio.2026.108361

Qiaoyu Han , Jiechao Liu , Ruifeng Wang , Hui Liu , Qiang Zhang , Zhenzhen Lv , Dalei Chen , Wenbo Yang , Zhonggao Jiao

This study was carried out to ascertain the behavior and fermentation performance of Lactobacillus plantarum 21802 in peach purees made from three different flesh-colored peach cultivars, and characterized the nutrition and flavor profiles of the various fermented purees by using HPLC, HS-SPME-GC/MS and E-nose. The results revealed that fermented peach purees from different flesh-colored cultivars exhibited distinct nutritional and sensory properties. The fermented white-fleshed peach puree had the highest total phenol content (199.39 μg/mL) and antioxidant activity (128.41 μg VC/mL). The yellow-fleshed fermented peach puree showed the highest total viable bacterial count and was rich in carotenoids (958.65 μg/100 mL), among which β-carotene (688.73 μg/100 mL) was the characteristic bioactive compound in this group. Additionally, the fermented red-fleshed peach puree was notable for its high levels of anthocyanins and aroma compounds compared to the other fermented groups, with total volatile organic compounds increasing 11.02-fold after fermentation. These findings demonstrate the benefits and characteristics of different flesh-colored peaches as raw materials for plant-based fermentation beverages, and provide strategies for developing diverse function probiotic fermented beverages.

本研究通过HPLC、HS-SPME-GC/MS、E-nose等方法，研究了植物乳杆菌21802在3种不同肉色桃果泥中的发酵行为和发酵性能，并对发酵后的桃果泥的营养和风味进行了表征。结果表明，不同肉色品种的发酵桃果泥具有不同的营养和感官特性。发酵后的桃果泥总酚含量最高（199.39 μg/mL），抗氧化活性最高（128.41 μg VC/mL）。黄肉发酵桃果泥的活菌总数最高，富含类胡萝卜素（958.65 μg/100 mL），其中β-胡萝卜素（688.73 μg/100 mL）是该组桃果泥的特征活性化合物。与其他发酵组相比，发酵后的红肉桃果浆具有较高的花青素和香气化合物含量，总挥发性有机化合物含量增加11.02倍。研究结果揭示了不同肉色桃子作为植物发酵饮料原料的优势和特点，为开发功能多样的益生菌发酵饮料提供了策略。

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引用次数: 0

Kinetic and spectroscopic studies on the inhibitory mechanisms of deoiled egg yolk hydrolysate against pancreatic lipase 蛋黄水解物对胰脂肪酶抑制机制的动力学和光谱研究

IF 5.9 1区农林科学 Q1 FOOD SCIENCE & TECHNOLOGY

Food Bioscience

Pub Date : 2026-03-01 Epub Date: 2026-01-23 DOI: 10.1016/j.fbio.2026.108307

Lin Zhang , Rui Cui , Wei Liu , Shasha Wang , Yifang Yan , Yi Wang , Xi Huang

Deoiled egg yolk powder (DOEP), rich in proteins and phospholipids, is underexploited due to its poor solubility. Therefore, this study employed enzymatic modification to enhance bioactivity, evaluated the in vitro hypolipidemic activity of DOEP hydrolysate (DOEPH), and elucidated the inhibitory mechanism of DOEPH on pancreatic lipase (PL). The results showed that DOEPH obtained under hydrolysis conditions (10 U/mg Trypsin for 4 h) exhibited a PL inhibition rate of 73.16 ± 4.81 % at a concentration of 10 mg/mL by a reversible mixed-type inhibition. Spectroscopic analysis further demonstrated that DOEPH reduced the intrinsic fluorescence of PL by 72.31 % at 37 °C, indicating that hydrophobic interactions drive conformational changes in PL, thereby decreasing its enzymatic activity. Two key components were screened via LC-MS/MS and molecular docking: Peptides (RIANQIR: 7.9 kcal/mol) and phosphatidylcholine (PC). RIANQIR bonded to a non-active site of PL, whereas PC interacted with the substrate active site (Arg-257), resulting in a dual synergistic inhibitory effect, which was consistent with the enzyme kinetics findings. Additionally, RIANQIR and PC (2 mg/mL) achieved PL inhibition rate of 50.00 ± 1.29 % and 47.84 ± 4.33 %, respectively, demonstrating their high hypolipidemic potential. This study offers a strategic approach for the high-value development and diversified industrial application of DOEP, while also proposing a novel dietary intervention for obesity management.

蛋黄去油粉富含蛋白质和磷脂，但其溶解度较差，未得到充分利用。因此，本研究采用酶修饰法增强其生物活性，评价DOEP水解物（DOEPH）的体外降血脂活性，阐明DOEPH对胰脂肪酶（PL）的抑制机制。结果表明，在10 U/mg Trypsin水解4 h的条件下，得到的DOEPH在10 mg/mL浓度下的PL抑制率为73.16±4.81%，呈可逆混合型抑制。光谱分析进一步表明，在37°C时，DOEPH使PL的本征荧光降低了72.31%，这表明疏水相互作用驱动了PL的构象变化，从而降低了其酶活性。通过LC-MS/MS和分子对接筛选两个关键成分：多肽（RIANQIR: 7.9 kcal/mol）和磷脂酰胆碱（PC）。RIANQIR与PL的非活性位点结合，而PC与底物活性位点（Arg-257）相互作用，产生双重协同抑制作用，这与酶动力学结果一致。此外，RIANQIR和PC （2 mg/mL）的PL抑制率分别为50.00±1.29%和47.84±4.33%，显示出较高的降血脂潜力。本研究为DOEP的高价值开发和多元化产业化应用提供了战略途径，同时也为肥胖管理提出了一种新的饮食干预方法。

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引用次数: 0

Molecular suppression of NLRP3 inflammasome by cyclic septapeptide in HCT116 cells for nutraceutical applications: In silico and in vitro analysis 环肽在HCT116细胞中对NLRP3炎性体的分子抑制，用于营养应用：硅和体外分析

IF 5.9 1区农林科学 Q1 FOOD SCIENCE & TECHNOLOGY

Food Bioscience

Pub Date : 2026-03-01 Epub Date: 2026-01-23 DOI: 10.1016/j.fbio.2026.108362

Shivanshu Nautiyal , Simran Singh , Shivika Srivastava , Muthiah Chellakkumar , Lakshmikanthan Hemajha , Akshad Balde , Soottawat Benjakul , Rasool Abdul Nazeer

Inflammation is a natural immune response to injury, infection, or harmful stimuli. While it is essential for host defense, chronic or excessive inflammation can lead to tissue damage and is implicated in diseases such as inflammatory bowel disease, gout, hyperuricemia nephropathy, and various pulmonary, hepatic, and neurological disorders. Certain oligopeptides, particularly those containing valine and proline, exhibit anti-inflammatory properties. Marine-derived oligopeptides are also known to possess diverse bioactive effects, including anticancer, antioxidative, and anti-inflammatory activities. In this study, an oligopeptide with the sequence CGQCPVS was cyclized via disulfide bond formation using the solid-phase peptide synthesis method. The cyclized peptide was analyzed and compared to its linear form using bioinformatics tools and molecular docking techniques to assess binding efficiency with key inflammatory proteins, including NLRP3, AIM2, Caspase-1, and IL-1β. The cyclized peptide exhibited reduced binding affinity to the NACHT domain of the NLRP3 inflammasome, comparable to MCC950, a known NLRP3 inhibitor. In vitro studies on the HCT116 epithelial cell line demonstrated high cell viability and a reduction in inflammatory markers at a 50 μM concentration. Further, the cyclized peptide downregulated proteins involved in the inflammatory pathway, including pro-inflammatory cytokines, effectively suppressing inflammation. These findings suggest that the cyclized oligopeptide holds potential as a pharmaceutical agent to mitigate NLRP3 inflammasome-associated inflammation and may serve as a promising candidate for developing novel anti-inflammatory therapies.

炎症是对损伤、感染或有害刺激的自然免疫反应。虽然它对宿主防御至关重要，但慢性或过度炎症可导致组织损伤，并与炎症性肠病、痛风、高尿酸血症肾病和各种肺部、肝脏和神经系统疾病等疾病有关。某些寡肽，特别是那些含有缬氨酸和脯氨酸的寡肽，具有抗炎特性。海洋来源的寡肽还具有多种生物活性，包括抗癌、抗氧化和抗炎活性。本研究采用固相肽合成方法，通过二硫键形成环化了序列为CGQCPVS的寡肽。利用生物信息学工具和分子对接技术对环化肽进行分析，并将其与线性形式进行比较，以评估与关键炎症蛋白（包括NLRP3、AIM2、Caspase-1和IL-1β）的结合效率。环化肽与NLRP3炎症小体NACHT结构域的结合亲和力降低，与已知的NLRP3抑制剂MCC950相当。体外研究表明，HCT116上皮细胞系在50 μM浓度下具有较高的细胞活力和炎症标志物的减少。此外，环化肽下调参与炎症途径的蛋白质，包括促炎细胞因子，有效抑制炎症。这些发现表明，环化寡肽作为一种药物制剂具有减轻NLRP3炎性小体相关炎症的潜力，并可能成为开发新型抗炎疗法的有希望的候选者。

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引用次数: 0

High-pressure processing combined with chitooligosaccharide-catechin conjugate enhances quality and shelf-life of precooked crab meat 高压加工结合壳寡糖-儿茶素偶联物，提高了预熟蟹肉的品质和保质期

IF 5.9 1区农林科学 Q1 FOOD SCIENCE & TECHNOLOGY

Food Bioscience

Pub Date : 2026-03-01 Epub Date: 2026-01-21 DOI: 10.1016/j.fbio.2026.108346

Khaettareeya Pimsannil , Suriya Palamae , Yu Fu , Bin Zhang , Jun Tae Kim , Nurul Huda , Soottawat Benjakul

Precooked blue swimming crab (P-BSC) meat is of high demand but it is perishable with short shelf-life. The effective preservation is still required to maintain the quality and ensure the safety. Therefore, the effects of chitooligosaccharide-catechin conjugate (COSC) (100 and 200 ppm) and high-pressure processing (HPP, 500 MPa for 5 min), individually and in combination, on the microbiological, chemical, and sensory quality of P-BSC lump meat during refrigerated storage (4 ± 1 °C) were investigated. COSC alone slightly delayed microbial growth, while HPP completely inhibited bacterial proliferation up to day 15. The combination of HPP and COSC, particularly HPP + COSC (200 ppm), extended the shelf-life up to 24 days by keeping aerobic plate count below 5 log CFU/g and effectively eliminating Pseudomonas and Vibrio spp. Chemical indicators of spoilage (pH, TVB-N, and TMA-N contents) remained within acceptable limits, and lipid oxidation (PV and TBARS) was significantly retarded. Principal component analysis confirmed strong separation between treated and untreated groups, indicating the efficacy of treatments used. EPA and DHA were not affected after extended storage, while volatile compounds containing spoilage-related acids and esters were reduced. Metagenomic profiling showed that HPP + COSC200 treatment suppressed spoilage and pathogenic taxa (Pseudoalteromonas, Shewanella, and Vibrio), while non-pathogenic Carnobacterium spp. became dominant. Sensory evaluation confirmed no detrimental effects on appearance, texture, odor, or overall acceptability after 24 days. Overall, the combined HPP + COSC200 treatment effectively preserved P-BSC lump meat and extended its shelf-life without compromising the eating quality.

预熟蓝蟹（P-BSC）肉需求量大，但易腐烂，保质期短。仍然需要有效的保存，以保持质量和确保安全。因此，研究了壳寡糖-儿茶素偶联物（COSC）（100和200 ppm）和高压处理（HPP, 500 MPa, 5 min）单独和联合处理对P-BSC块肉冷藏（4±1℃）期间微生物学、化学和感官品质的影响。单独使用COSC可轻微延缓微生物生长，而HPP可完全抑制细菌增殖至第15天。HPP和COSC的组合，特别是HPP + COSC (200 ppm)，通过将好氧平板计数保持在5 log CFU/g以下，有效地去除假单胞菌和弧菌，延长了保质期长达24天，变质的化学指标（pH， TVB-N和TMA-N含量）保持在可接受的范围内，脂质氧化（PV和TBARS）显著延缓。主成分分析证实了治疗组和未治疗组之间的强烈分离，表明所使用的治疗方法的有效性。EPA和DHA在长时间储存后不受影响，而含有腐败相关酸和酯的挥发性化合物则减少了。宏基因组分析显示，HPP + COSC200处理抑制了腐败和致病分类群（假互变单胞菌、希瓦氏菌和弧菌），而非致病性肉杆菌成为优势。24天后，感官评估证实对外观、质地、气味或总体可接受性没有不利影响。总体而言，HPP + COSC200联合处理有效地保存了P-BSC块肉，延长了其保质期，而不影响食用质量。

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引用次数: 0

A novel antioxidant system in Lactococcus lactis N8 乳酸乳球菌N8中一种新的抗氧化系统

IF 5.9 1区农林科学 Q1 FOOD SCIENCE & TECHNOLOGY

Food Bioscience

Pub Date : 2026-03-01 Epub Date: 2026-01-22 DOI: 10.1016/j.fbio.2026.108358

Huan Yang , Xian Xu , Fengming Liu , Duodong Wang , Yongping Xin , Mingqiang Qiao

The ability of Lactococcus lactis to grow under moderate oxygen conditions enables its expansion from the food industry to microbial cell factories. While leveraging known antioxidative regulatory mechanisms represents a promising strategy for engineering robust strains under high oxygen conditions, the complete genetic basis of oxygen tolerance in L. lactis remains incompletely understood. Here, we identified and characterized a novel antioxidative system and its corresponding regulatory mechanism in L. lactis N8. Genetic analyses demonstrated that RmaH, a MarR-family transcriptional regulator, is essential for mediating the oxidative stress response. Transcriptomic and qPCR analysis further revealed that RmaH acts as a transcriptional repressor of dps, which encodes a DNA-binding protein from starved cells (Dps). In vitro and in vivo protein-DNA binding assays confirmed that RmaH specifically binds to the coding sequence (CDS) of dps, with DNase I footprinting precisely identifying the binding motif (TGTAAG-12nt-CTTTCA). Finally, functional investigations revealed that under oxygen conditions, RmaH expression was suppressed, leading to upregulated dps expression. Dps thereby confers cellular protection via two distinct mechanisms: physically shielding DNA from hydroxyl radicals and inhibiting the Fenton reaction through its ferroxidase activity. Collectively, our findings elucidated a previously uncharacterized RmaH-Dps regulatory pathway that enhances oxygen tolerance in L. lactis, providing both mechanistic insight and a potential target for engineering industrially robust strains.

乳酸乳球菌在中等氧条件下生长的能力使其从食品工业扩展到微生物细胞工厂。虽然利用已知的抗氧化调节机制是在高氧条件下设计健壮菌株的一种有前途的策略，但乳酸菌耐氧的完整遗传基础仍不完全清楚。本研究鉴定了乳酸菌N8中一个新的抗氧化系统及其调控机制。遗传分析表明，RmaH，一个marr家族转录调节因子，在介导氧化应激反应中是必不可少的。转录组学和qPCR分析进一步揭示RmaH作为dps的转录抑制因子，其编码来自饥饿细胞（dps）的dna结合蛋白。体外和体内蛋白- dna结合实验证实RmaH特异性结合dps的编码序列（CDS）， DNase I足迹精确识别结合基序（TGTAAG-12nt-CTTTCA）。最后，功能研究显示，在氧气条件下，RmaH表达被抑制，导致dps表达上调。因此，Dps通过两种不同的机制提供细胞保护：物理上屏蔽DNA免受羟基自由基的侵害，并通过其铁氧化酶活性抑制芬顿反应。总的来说，我们的研究结果阐明了一个以前未被表征的RmaH-Dps调节途径，该途径增强了乳杆菌的氧耐受性，为工程工业健壮菌株提供了机制见解和潜在目标。

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引用次数: 0

Improving Penaeus monodon survival during live transport: Gradient cooling acclimation reduces stress and enhances immunity through proteomic modulation 提高单对虾在活体运输中的存活率：梯度冷却驯化通过蛋白质组调节减少应激和增强免疫力

IF 5.9 1区农林科学 Q1 FOOD SCIENCE & TECHNOLOGY

Food Bioscience

Pub Date : 2026-03-01 Epub Date: 2026-01-22 DOI: 10.1016/j.fbio.2026.108332

Kun Ren , Mengtong Wu , Tianle Cao , Longteng Zhang , Chuan Li , Xiaoshuan Zhang , Yanfu He

Live transportation of Penaeus monodon is critical for preserving shrimp quality in aquaculture operations, yet conventional transport without dormancy induction often results in severe stress and mortality. However, studies investigating the stress response and regulatory adaptations of P. monodon during transport remain limited. This study examined the impact of low-temperature acclimation on the transport survival of P. monodon, compared to traditional non-cooled (WC) storage. Water quality parameters, physiological, biochemical, and proteomic responses were analyzed. The WC group showed a significant decline in dissolved oxygen (DO) levels and an increase in ammonia nitrogen concentration; hemolymph glucose levels progressively declined, whereas catalase (CAT), alkaline phosphatase (ALP), and superoxide dismutase (SOD) activities initially increased before decreasing sharply; lactate (LA) accumulation was severe, and survival rates dropped to 5 % after 4 h of live transport. In contrast, low-temperature acclimation improved physiological status, with the GC group showing optimal performance, including a 56.5 % reduction in glucose consumption rate during the first 4 h compared to WC; endpoint ALP activity of gradient cooling (GC) was 136.2 % of that in the WC group and 108.5 % of that in the acute cooling (AC) group, respectively; CAT and SOD activities remained stably elevated, whereas the other groups showed significant late-stage declines. Proteomic analysis revealed that the AC group upregulated proteins associated with oxidative phosphorylation, ATP metabolism, stress responses, and apoptosis, while the GC group promoted the expression of glycolysis- and immune-related proteins. GC acclimation improved the transport survival of P. monodon by reducing metabolism, mitigating stress, and enhancing immunity.

在水产养殖作业中，单节对虾的活体运输对于保持对虾质量至关重要，但不进行休眠诱导的传统运输往往导致严重的应激和死亡。然而，关于单胞单胞菌在运输过程中的应激反应和调节适应性的研究仍然有限。这项研究调查了影响低温适应运输p .他们的生存,相比传统型无冷(WC)存储。分析水质参数、生理生化和蛋白质组学反应。WC组溶解氧（DO）水平显著下降，氨氮浓度显著升高；血淋巴葡萄糖水平逐渐下降，过氧化氢酶（CAT）、碱性磷酸酶（ALP）和超氧化物歧化酶（SOD）活性先升高后急剧下降；乳酸积累严重，活体运输4小时后存活率降至5%。相比之下，低温驯化改善了生理状态，GC组表现最佳，包括前4小时葡萄糖消耗率比WC降低56.5%；终点ALP活性梯度冷却（GC）组分别为WC组的136.2%和急性冷却（AC）组的108.5%；CAT和SOD活性保持稳定升高，而其他组则表现出明显的后期下降。蛋白质组学分析显示，AC组上调了与氧化磷酸化、ATP代谢、应激反应和凋亡相关的蛋白，而GC组促进了糖酵解和免疫相关蛋白的表达。GC驯化通过降低代谢、减轻应激和增强免疫力来提高单胞假单胞菌的转运存活率。

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引用次数: 0