Food Bioscience最新文献_第6页

Natural antimicrobials as sustainable preservatives against postharvest microbial spoilage in fruits and vegetables 天然抗菌剂作为水果和蔬菜采后微生物腐败的可持续防腐剂

IF 5.9 1区农林科学 Q1 FOOD SCIENCE & TECHNOLOGY

Food Bioscience

Pub Date : 2026-03-01 Epub Date: 2026-01-29 DOI: 10.1016/j.fbio.2026.108379

Aqleem Abbas, Yanyin Guo, Nana Ji, Shanshan Hao, Huihui Xu, Muhammad Asim

Postharvest microbial spoilage caused by fungi, bacteria, and yeasts results in 30 %–40 % of global losses in fruits and vegetables (FVs), driven by their elevated water activity, near-neutral pH, nutrient richness, and physiological changes associated with senescence. The global food preservatives market, valued at USD 2.9 billion in 2023 and projected to reach USD 4.16 billion by 2030, is predominantly controlled by synthetic preservatives, which account for a 69 % market share. Despite their widespread applications, synthetic preservatives face increasing limitations due to microbial resistance, regulatory restrictions, residue-related safety concerns, environmental toxicity, and growing consumer demand for clean-label produce. In this review, we evaluate natural antimicrobials derived from plants, microorganisms, algae, and animals as safe, biodegradable natural preservatives. These compounds exhibit multi-target antimicrobial mechanisms, including membrane disruption, bioenergetic collapse, oxidative stress induction, and quorum-sensing inhibition, which considerably diminishes the likelihood of resistance in contrast to single-target synthetic preservatives. Advances in delivery strategies, such as edible coatings, active packaging, nanoencapsulation, and multi-hurdle preservation systems, have significantly enhanced the stability, efficacy, and practical applicability of natural antimicrobials in FVs by mitigating limitations related to volatility and sensory impact. This review demonstrates that the growing potential of natural antimicrobials to serve as effective and sustainable postharvest preservatives for FVs, while recognizing that further optimization and scale-up are required for widespread commercial adoption.

由真菌、细菌和酵母引起的采后微生物腐败导致全球水果和蔬菜（FVs）损失的30% - 40%，其驱动因素是水分活性升高、pH值接近中性、营养丰富以及与衰老相关的生理变化。全球食品防腐剂市场在2023年价值29亿美元，预计到2030年将达到41.6亿美元，主要由合成防腐剂控制，占69%的市场份额。尽管合成防腐剂得到了广泛的应用，但由于微生物耐药性、监管限制、残留物相关的安全问题、环境毒性以及消费者对清洁标签产品日益增长的需求，合成防腐剂面临着越来越多的限制。在这篇综述中，我们评估了从植物、微生物、藻类和动物中提取的天然抗菌剂是安全的、可生物降解的天然防腐剂。这些化合物表现出多靶点抗菌机制，包括膜破坏、生物能量崩溃、氧化应激诱导和群体感应抑制，与单靶点合成防腐剂相比，这大大降低了耐药性的可能性。可食用涂层、活性包装、纳米封装和多栏保存系统等递送策略的进步，通过减轻与挥发性和感官影响相关的限制，显著提高了天然抗菌剂在生物燃料中的稳定性、有效性和实际适用性。这篇综述表明，天然抗菌剂作为野生动物有效和可持续的采后防腐剂的潜力越来越大，同时认识到需要进一步优化和扩大规模才能广泛用于商业应用。

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引用次数: 0

Human milk oligosaccharides: Overcoming production bottlenecks through novel large-scale strategies and applications 人乳低聚糖：通过新的大规模策略和应用克服生产瓶颈

IF 5.9 1区农林科学 Q1 FOOD SCIENCE & TECHNOLOGY

Food Bioscience

Pub Date : 2026-03-01 Epub Date: 2026-02-02 DOI: 10.1016/j.fbio.2026.108405

Hatice Duman , Ayşe Nur Yurdcu , Ateş Kara , Mikhael Bechelany , Sercan Karav

Breast milk comprises nutrient-dense bioactive constituents and a diversive bacterial population that encourages gut microbiota maturation, enhances immunological function, and aids in pathogen defense. Human milk oligosaccharides (HMOs), the third most abundant solid component, are key bioactive carbohydrates that shape intestinal microbiota, provide antibacterial effects, enhance the intestinal barrier, and regulate immune responses. For many years, research in this area has been constrained by the restricted availability of HMOs. Only human milk contains the majority of HMOs, and historically, it has been costly and time-consuming to isolate or synthesize them. This review examines novel approaches to mitigate production bottlenecks in HMOs, emphasizing chemoenzymatic synthesis, microbial engineering, and extraction from donor milk or dairy sources. It highlights progress in synthetic biology that improves biosynthetic pathways, increasing catalytic efficiency, stability, and diversity of intricate HMO structures. It evaluates the industrial scalability and technoeconomic feasibility of plant-based and microbial systems as economical and sustainable approaches for large-scale HMO production.

母乳含有营养丰富的生物活性成分和多样化的细菌种群，促进肠道微生物群成熟，增强免疫功能，并有助于病原体防御。母乳低聚糖（HMOs）是第三丰富的固体成分，是塑造肠道微生物群、提供抗菌作用、增强肠道屏障和调节免疫反应的关键生物活性碳水化合物。多年来，这一领域的研究一直受到hmo有限可用性的限制。只有人乳含有大多数hmo，而且从历史上看，分离或合成它们既昂贵又耗时。这篇综述探讨了缓解hmo生产瓶颈的新方法，强调了化学酶合成、微生物工程和从供体牛奶或乳制品来源中提取。它突出了合成生物学的进展，改善了生物合成途径，提高了催化效率，稳定性和复杂的HMO结构的多样性。它评估了植物和微生物系统作为大规模HMO生产的经济和可持续方法的工业可扩展性和技术经济可行性。

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引用次数: 0

pH-driven assembly of soy proteins into nanoparticles for efficient curcumin delivery: Role of β-conglycinin and glycinin subunits ph驱动的大豆蛋白组装成有效姜黄素递送的纳米颗粒：β-甘氨酸和甘氨酸亚基的作用

IF 5.9 1区农林科学 Q1 FOOD SCIENCE & TECHNOLOGY

Food Bioscience

Pub Date : 2026-03-01 Epub Date: 2026-01-27 DOI: 10.1016/j.fbio.2026.108368

Wen-Wen Zhu, Chuan-He Tang

Curcumin has garnered significant attention due to its multifaceted health benefits, yet the poor physicochemical properties limit broader applications. The pH-shift method was employed to induce the formation of soy nanoparticles and achieve efficient encapsulation of curcumin, with a specific focus on elucidating the distinct roles of the major subunits, β-conglycinin (7 S) and glycinin (11 S). Systematic comparison revealed that the dissociation and reassembly behaviors of soy protein isolate (SPI), 7 S, and 11 S were governed by their subunit-specific properties, particularly hydrophilicity/hydrophobicity, glycosylation and surface charge distribution. Specifically, the more hydrophilic glycosylated subunits of 7 S (α, α′) were able to inhibit excessive aggregation during dissociation, ultimately reassembling into more compact 7 S nanoparticles with highly charged and moderate surface hydrophobicity (30.62 nm, −32.2 mV), driven by hydrophobic interactions, electrostatic forces, and hydrogen bonding. The improved structural properties and extensive exposure of hydrophobic regions enabled a high curcumin loading capacity (∼93 %) and significantly enhanced thermal, digestive and storage stability. This study elucidates the application potential and molecular mechanisms of complex soy protein structures as active ingredient delivery carriers, aiming to provide new insights for designing efficient, plant-based nano-delivery systems for bio-actives.

姜黄素因其多方面的健康益处而受到广泛关注，但其较差的理化性质限制了其更广泛的应用。采用ph位移法诱导形成大豆纳米颗粒并实现姜黄素的有效包封，重点阐明了主要亚基β-conglycinin （7 S）和glycinin （11 S）的不同作用。系统比较表明，大豆分离蛋白（SPI）、大豆分离蛋白7s和大豆分离蛋白11s的解离和重组行为受其亚基特异性，特别是亲疏水性、糖基化和表面电荷分布的影响。具体来说，在疏水相互作用、静电力和氢键的驱动下，更亲水的7s糖基化亚基（α, α '）能够在解离过程中抑制过度聚集，最终重组成更致密的7s纳米粒子，具有高电荷和中等表面疏水性（30.62 nm,−32.2 mV）。改进的结构特性和疏水区的广泛暴露使姜黄素的负载能力高（~ 93%），并显著提高热、消化和储存稳定性。本研究阐明了复杂大豆蛋白结构作为活性成分递送载体的应用潜力和分子机制，旨在为设计高效的植物基生物活性物质纳米递送系统提供新的见解。

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引用次数: 0

Study on the structure-activity relationship and effect evaluation of benzo -γ -pyrones anti-S. aureus Hla inhibitors 苯并-γ -吡咯酮类抗s的构效关系及效果评价研究。金黄色葡萄球菌Hla抑制剂

IF 5.9 1区农林科学 Q1 FOOD SCIENCE & TECHNOLOGY

Food Bioscience

Pub Date : 2026-03-01 Epub Date: 2026-01-29 DOI: 10.1016/j.fbio.2026.108330

Tingting Sun, Lin Jiang, Lijiao Yang, Siyi Chen, Yang Liu, Jing Liu, Fei Xu

Benzo-γ-pyrone derivatives are widely utilized in both the food industry and pharmaceutical research. From a constructed Benzo-γ-pyrone Natural Compounds Database (BNCD), we screened 18 compounds with the core benzo-γ-pyrone skeleton against Staphylococcus aureus α-hemolysin (Hla) and categorized them into five classes (A-E). We identified 10 effective inhibitors by hemolytic assay that could be pooled with the use of a dose-response model to generate an IC₅₀ curve. Dose-response analysis yielded IC₅₀ values, which, together with molecular docking results for the Hla secretion regulators (AgrC, AgrA, SarR, SarA), revealed a key pharmacophore. The optimal structure is characterized by a benzo-γ-pyrone backbone featuring a C2-linked B-ring, an essential 5,7-dihydroxy motif on the A-ring, and a B-ring substituted with either a 4′-hydroxyl or a 3′,4′-catechol group. The minimum inhibitory concentrations (MICs) of morin, apigenin, (+/−)-naringenin, and liquiritigenin were all >2000 μg/mL. Subsequent cytotoxicity assessments (CCK-8 assay) revealed no significant cytotoxicity (P > 0.05) for morin and apigenin up to 2000 μg/mL, for naringenin up to 500 μg/mL, and for liquiritigenin up to 250 μg/mL in bovine mammary epithelial cells. Furthermore, these compounds conferred concentration-dependent protection against S. aureus-induced damage in both in vitro Raw264.7 macrophage and in vivo Galleria mellonella larval infection models. In conclusion, our study identifies specific dietary benzo-γ-pyranones as promising non-antibiotic, anti-virulence agents targeting S. aureus Hla. These findings provide a rational basis for designing functional foods and developing novel anti-virulence therapeutics.

苯并γ-吡咯酮衍生物在食品工业和制药研究中都有广泛的应用。从已构建的苯并γ-吡咯酮天然化合物数据库（BNCD）中筛选出18种具有抗金黄色葡萄球菌α-溶血素（Hla）核心骨架的化合物，并将其分为5类（a - e）。我们通过溶血试验确定了10种有效的抑制剂，可以使用剂量-反应模型进行合并，以产生IC50曲线。剂量-反应分析得到IC50值，并结合Hla分泌调节因子（AgrC、AgrA、SarR、SarA）的分子对接结果，揭示了一个关键药效团。最优结构为：苯并γ-吡咯酮骨架上有一个c2连接的b环，a环上有一个必需的5,7-二羟基基序，b环被一个4 ' -羟基或3 ',4 ' -儿茶酚基取代。桑里素、芹菜素、(+/−)-柚皮素、利尿素的最低抑制浓度均为2000 μg/mL。随后的细胞毒性评估（CCK-8试验）显示，对牛乳腺上皮细胞，桑皮苷和芹菜素高达2000 μg/mL，柚皮苷高达500 μg/mL，利尿素高达250 μg/mL均无显著的细胞毒性（P > 0.05）。此外，在体外Raw264.7巨噬细胞和体内mellonella幼虫感染模型中，这些化合物对金黄色葡萄球菌诱导的损伤具有浓度依赖的保护作用。总之，我们的研究确定了特定的膳食苯并γ-吡喃酮是一种很有前途的非抗生素、针对金黄色葡萄球菌Hla的抗毒剂。这些发现为设计功能性食品和开发新的抗毒药物提供了合理的依据。

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引用次数: 0

Pulsed electric fields selectivity in peel phenolics from Actinidia deliciosa vs. Opuntia ficus-indica: Triple TOF-LC-MS/MS profiling for valorization 脉冲电场对猕猴桃果皮酚类物质的选择性：三重TOF-LC-MS/MS谱分析

IF 5.9 1区农林科学 Q1 FOOD SCIENCE & TECHNOLOGY

Food Bioscience

Pub Date : 2026-03-01 Epub Date: 2026-02-05 DOI: 10.1016/j.fbio.2026.108423

Mara Calleja-Gómez , Albert Sebastià , José M. Lorenzo , Mirian Pateiro , Noelia Pallarés

Fruit processing generates large amounts of peel by-products that represent underexploited sources of phenolic compounds with antioxidant potential. This study applied pulsed electric fields technology (PEF) as a green, water-based extraction strategy to valorize kiwifruit (Actinidia deliciosa) and prickly pear (Opuntia ficus-indica) peels, to determine matrix-specific optimal conditions and assess the selective release of bioactives. A response surface methodology was employed to optimize field strength (kV/cm), specific energy (kJ/kg), and maceration time (h), followed by characterization of the extracts: phenolic content, antioxidant capacity (ORAC, TEAC), pigments, macronutrients, minerals (ICP-MS), and phenolic profiles by LC-QTOF-MS. PEF enhanced phenolic recovery and antioxidant capacity compared to conventional maceration, although the magnitude of improvement was strongly dependent on the matrix. Kiwifruit peel benefited from higher energy inputs, resulting in increased release of phenolics (42.4%), antioxidant capacity (61.8%), and chlorophylls (72.7%), while prickly pear peel achieved better outcomes under low-energy conditions, with moderate increases in phenolic content (30.6%) and antioxidant capacity (39.4%). LC-QTOF-MS confirmed that PEF promoted the release of procyanidins and catechin in kiwi, while preserved kaempferol and quercetin derivatives in prickly pear. Mineral solubilization displayed matrix-dependent trends, with kiwi extracts enriched in Fe, P and K (up to 94.8%), and prickly pear extracts in K, Mg and Cu (up to 95.1%). Overall, PEF should be regarded not simply as an intensification tool, but as a selective technology that requires parameter adjustment according to tissue structure and targeted compound class. This matrix-tailored approach supports the sustainable valorization of fruit peels into antioxidant-rich ingredients.

水果加工过程中会产生大量果皮副产品，这些副产品代表了具有抗氧化潜力的酚类化合物未被充分利用的来源。本研究应用脉冲电场技术（PEF）作为绿色水基提取策略，对猕猴桃（Actinidia deliciosa）和刺梨（Opuntia ficus-indica）果皮进行了纯化，以确定基质特异性的最佳条件，并评估生物活性物质的选择性释放。采用响应面法优化其场强（kV/cm）、比能（kJ/kg）和浸渍时间(h)，并采用LC-QTOF-MS表征其酚类含量、抗氧化能力（ORAC、TEAC）、色素、常量营养素、矿物质（ICP-MS）和酚类特征。与传统浸渍相比，PEF提高了酚的回收率和抗氧化能力，尽管改善的程度强烈依赖于基质。猕猴桃果皮在高能量投入下，酚类物质释放量增加（42.4%），抗氧化能力增加（61.8%），叶绿素释放量增加（72.7%），而刺梨果皮在低能量投入下效果更好，酚类物质含量增加（30.6%），抗氧化能力增加（39.4%）。LC-QTOF-MS证实，PEF促进了猕猴桃中原花青素和儿茶素的释放，保留了刺梨中山奈酚和槲皮素衍生物的释放。矿物质增溶表现出基质依赖性，猕猴桃提取物的Fe、P和K含量最高达94.8%，刺梨提取物的K、Mg和Cu含量最高达95.1%。总的来说，PEF不应被简单地视为一种强化工具，而应被视为一种选择性技术，需要根据组织结构和靶向化合物类别调整参数。这种基质定制的方法支持果皮的可持续增值成为富含抗氧化剂的成分。

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引用次数: 0

Biopolymer-based strategies to improve delivery properties of B12 in alginate gels: Role of protein fillers and chitosan 以生物聚合物为基础的策略来改善藻酸盐凝胶中B12的输送特性：蛋白质填料和壳聚糖的作用

IF 5.9 1区农林科学 Q1 FOOD SCIENCE & TECHNOLOGY

Food Bioscience

Pub Date : 2026-03-01 Epub Date: 2026-01-28 DOI: 10.1016/j.fbio.2026.108381

Raquel de Freitas Agondi, Ludmila Meskelis, Larissa Graziele Rauber Duarte, Ana Carla Kawazoe Sato, Carolina Siqueira Franco Picone

Alginate is a natural biopolymer widely used for enteric delivery of bioactive, due to its biocompatibility, low toxicity, and mild gelation abilities. The encapsulation efficiency of bioactive compounds in alginate matrix is straight related to the gel pore size that can be tuned using fillers. Herin, we propose a new approach for improving the encapsulation and delivery of cyanocobalamin (vitamin B12) in alginate gels by filling the network with different sources of proteins. Vegetable fillers (rice and potato proteins) were compared to whey protein fillers on enhancing the physical and delivery properties of alginate particles formed by ionotropic gelation. The effect of filler type on the stability of the particles at different pH values, as well as on the encapsulation efficiency of cyanocobalamin and its release was compared to traditional alginate-chitosan complexation. The encapsulation efficiency of pure alginate gels improved from 16 % to 86 % with rice protein filler. Although rice and potato protein-filled gels presented similar release profiles, they diverge in mechanical properties and delayed the bioactive release in comparison to whey protein-filled gels. The complexation with chitosan affected the vitamin release and increased particle stability at pH 3.0. Alginate beads filled with vegetable proteins and complexed with chitosan reached 93 % of vitamin encapsulation. These results highlight the improvement of alginate matrix based on vegetable proteins and expand its uses as delivery systems of hydrophilic bioactive compounds, such as vitamin B12.

海藻酸盐是一种天然的生物聚合物，由于其生物相容性、低毒性和温和的凝胶能力，被广泛用于肠内生物活性递送。生物活性化合物在海藻酸盐基质中的包封效率与凝胶孔隙大小直接相关，凝胶孔隙大小可以通过填料调节。在这里，我们提出了一种新的方法，通过在海藻酸盐凝胶中填充不同来源的蛋白质来改善氰钴胺素（维生素B12）的包封和递送。比较了植物填料（大米和马铃薯蛋白）与乳清蛋白填料在增强海藻酸盐颗粒的物理和输送性能方面的差异。比较了填料类型对不同pH值下颗粒稳定性的影响，以及对氰钴胺素包封率和释放量的影响。用大米蛋白填料将纯海藻酸盐凝胶的包封率从16%提高到86%。虽然大米和马铃薯蛋白填充凝胶具有相似的释放特征，但与乳清蛋白填充凝胶相比，它们在机械特性上存在差异，并延迟了生物活性释放。壳聚糖的络合作用影响了维生素的释放，提高了pH为3.0时的颗粒稳定性。海藻酸盐填充植物蛋白并与壳聚糖络合，达到93%的维生素包封。这些结果强调了基于植物蛋白的藻酸盐基质的改进，并扩大了其作为亲水性生物活性化合物（如维生素B12）的递送系统的应用。

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引用次数: 0

Jaboticaba peel: chemical composition, phenolic bioaccessibility, and health benefits 野蕉皮：化学成分、酚类生物可及性和健康益处

IF 5.9 1区农林科学 Q1 FOOD SCIENCE & TECHNOLOGY

Food Bioscience

Pub Date : 2026-03-01 Epub Date: 2026-02-07 DOI: 10.1016/j.fbio.2026.108440

Roberto de Paula do Nascimento , Marina Moreira de Castro , Paola Melisa Calderon Navarrete , Mario Roberto Marostica Junior , Raquel Franco Leal

Jaboticaba (Plinia spp.) is a berry native to Brazil's Atlantic Forest, recognized for its rich phytochemical composition and therapeutic potential. Among the fruit's fractions, the peel is particularly valuable, containing the highest concentration of phenolics, with anthocyanins, ellagitannins, and phenolic acids as the predominant compounds. In addition, the dried peel contains a high concentration of dietary fiber, especially insoluble fiber. Recent research has focused on the bioaccessibility of jaboticaba peel polyphenols, revealing that many compounds undergo transformation during digestion, producing derivatives that are readily absorbed or reach the colon, where they are metabolized by gut microbiota. Such microbial catabolism has been associated with modulation of microbiota composition and function, as well as activation of signaling pathways linked to the prevention and management of chronic inflammatory conditions. Furthermore, studies indicate that jaboticaba peel can exert systemic effects, including improvements in metabolic, cardiovascular, and immune health. Despite growing interest, a comprehensive synthesis of the chemical composition, bioaccessibility, and biological effects of jaboticaba peel remains limited. This review aims to fill this gap by presenting a detailed analysis of the peel's key phytochemicals, their digestive transformation, and their potential health benefits. Understanding these aspects is crucial to highlight the peel not only as a byproduct of fruit consumption but also as a promising source of bioactive compounds for functional foods, nutraceuticals, and therapeutic applications. By integrating current knowledge, this work underscores the relevance of jaboticaba peel in nutrition and health research, providing a foundation for future studies and potential clinical applications.

Jaboticaba （Plinia spp.）是一种原产于巴西大西洋森林的浆果，以其丰富的植物化学成分和治疗潜力而闻名。在水果的部分中，果皮特别有价值，含有最高浓度的酚类物质，以花青素、鞣花单宁和酚酸为主要化合物。此外，干果皮含有高浓度的膳食纤维，尤其是不溶性纤维。最近的研究集中在牙买加豆皮多酚的生物可及性上，揭示了许多化合物在消化过程中发生转化，产生易于吸收或到达结肠的衍生物，在那里它们被肠道微生物群代谢。这种微生物分解代谢与微生物群组成和功能的调节以及与慢性炎症的预防和管理相关的信号通路的激活有关。此外，研究表明，香蕉皮可以发挥全身作用，包括改善代谢、心血管和免疫健康。尽管越来越多的兴趣，综合合成的化学成分，生物可及性和生物效应的烟草皮仍然有限。这篇综述旨在通过详细分析果皮的关键植物化学物质，它们的消化转化，以及它们潜在的健康益处来填补这一空白。了解这些方面对于强调果皮不仅是水果消费的副产品，而且是功能性食品，营养药品和治疗应用的生物活性化合物的有前途的来源至关重要。通过整合现有的知识，本工作强调了古巴蕉皮在营养和健康研究中的相关性，为未来的研究和潜在的临床应用提供了基础。

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引用次数: 0

Pulsed electric field effects on plant matrices: A comprehensive review of structural and textural responses 脉冲电场对植物基质的影响：结构和结构响应的综合综述

IF 5.9 1区农林科学 Q1 FOOD SCIENCE & TECHNOLOGY

Food Bioscience

Pub Date : 2026-03-01 Epub Date: 2026-02-02 DOI: 10.1016/j.fbio.2026.108402

Neamtallah Assaf, Emma Chiavaro, Massimiliano Rinaldi

The growing consumer demand for high-quality, safe, and nutritious food products has driven the food industry to explore innovative processing technologies. Rapid lifestyle changes and an increased preference for fresh, minimally processed foods challenge traditional preservation methods, which often struggle to meet efficiency and quality expectations. In response, novel, cost-effective, and environmentally sustainable techniques have emerged, focusing on maintaining sensory attributes, nutritional value, and food safety. Among these, Pulsed Electric Fields (PEF) technology shows promising applications in food processing and functional food production. This review aims to provide a comprehensive overview of PEF applications in various plant materials, emphasizing its effects on cellular structures, mechanisms, overall product quality and relevance for industrial processing applications. Relevant changes discussed include structural modifications including cell size and shape, elongation index, cell wall integrity, and cell viability, along with textural properties such as hardness and firmness. Other key parameters include cell disintegration index, mass transfer, ion leakage, and uptake. Additionally, modifications in starch and pectin components have been described. The review further discusses the implications of these structural changes for industrial processing efficiency, particularly in relation to mass transfer, texture, and energy-efficient operations, and considers the impact of other emerging non-thermal technologies alongside PEF.

消费者对高质量、安全和营养食品的需求不断增长，促使食品行业探索创新的加工技术。生活方式的快速变化和对新鲜、最低限度加工食品的日益偏好挑战了传统的保存方法，这些方法往往难以满足效率和质量的期望。因此，新的、具有成本效益和环境可持续性的技术出现了，重点是保持感官属性、营养价值和食品安全。其中，脉冲电场（PEF）技术在食品加工和功能食品生产中具有广阔的应用前景。本文旨在全面综述PEF在各种植物材料中的应用，重点介绍其对细胞结构、机制、整体产品质量的影响及其在工业加工中的应用。所讨论的相关变化包括结构变化，包括细胞大小和形状、延伸指数、细胞壁完整性和细胞活力，以及硬度和硬度等质地特性。其他关键参数包括细胞解体指数、传质、离子泄漏和摄取。此外，还描述了淀粉和果胶组分的改性。该综述进一步讨论了这些结构变化对工业加工效率的影响，特别是与传质、质地和节能操作有关的影响，并考虑了其他新兴的非热技术与PEF的影响。

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引用次数: 0

Curcumin cellular delivery based on assembly of clover shaped trityl polyethylene glycols with ovalbumin 基于三叶草形聚乙二醇与卵白蛋白组装的姜黄素细胞递送

IF 5.9 1区农林科学 Q1 FOOD SCIENCE & TECHNOLOGY

Food Bioscience

Pub Date : 2026-03-01 Epub Date: 2026-01-29 DOI: 10.1016/j.fbio.2026.108382

Zhe Zheng, Songbai Liu

Novel curcumin (Cur) cellular delivery system based on assembly of clover shaped trityl polyethylene glycol (TrPEG) with heat denatured ovalbumin (OVA) through hydrophobic interactions was developed in this study. TrPEGs of different degree polymerization (n = 3, 6, 9) were prepared by the coupling of trityl chloride and PEGs under the optimized condition, and the structures were verified by NMR and MS measurements. The excellent amphiphilic and biocompatible TrPEG-9 demonstrated marvelous selective binding capability with the denatured OVA and stabilization of the assembled particles through the clover shaped head. Introduction of TrPEG-9 (EG) with OVA significantly enhanced encapsulation efficiency (83 %) of Cur and afforded uniform compact nanoparticles (63 nm). Evaluation by Saccharomyces cerevisiae model revealed effectively promoted Cur cellular delivery and significantly reactive oxygen species (ROS) reduction capacity of EG-OVA-Cur, and clathrin-mediated endocytosis and macropinocytosis cellular uptake followed by intracellular vacuolar trafficking. This assembled system has great potential for cellular delivery of nutraceutical and biomedical bioactives.

本研究基于三叶草型聚乙二醇（TrPEG）与热变性卵清蛋白（OVA）通过疏水相互作用组装而成的新型姜黄素（Cur）细胞递送体系。在优化条件下，将三氯甲烷与聚乙二醇偶联制备了不同聚合度的trpeg (n = 3,6,9)，并通过NMR和MS对结构进行了验证。TrPEG-9具有良好的两亲性和生物相容性，与变性OVA具有良好的选择性结合能力，并通过三叶草形状的头部稳定组装颗粒。用OVA引入TrPEG-9 （EG）显著提高了Cur的包封效率（83%），并提供了均匀致密的纳米颗粒（63 nm）。通过酿酒酵母模型评估发现，egg - ova -Cur可有效促进Cur的细胞传递和显著的活性氧（ROS）还原能力，以及网格蛋白介导的内吞和巨噬细胞摄取以及随后的细胞内液泡运输。这种组装系统在营养和生物医学生物活性物质的细胞递送方面具有巨大的潜力。

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引用次数: 0

Dietary lipids synergistically enhance PAH bioavailability and intestinal toxicity: Mechanistic insights from a Caco-2 model 膳食脂类协同提高多环芳烃的生物利用度和肠道毒性：Caco-2模型的机制见解

IF 5.9 1区农林科学 Q1 FOOD SCIENCE & TECHNOLOGY

Food Bioscience

Pub Date : 2026-03-01 Epub Date: 2026-01-26 DOI: 10.1016/j.fbio.2026.108374

Xiaofang Liu , Jie Zhu , Wanyi Zou , Li Liang , Jixian Zhang , Chaoting Wen , Youdong Li , Guoyan Liu , Xin Xu

This study employed a Caco-2 intestinal model to evaluate the impact of soybean oil and its fatty acid constituents (oleic acid (OA), linoleic acid (LA), and linolenic acid (LNA)) on the intestinal absorption, transport, and toxicity of benzo[a]pyrene (BaP) and benzo[b]fluoranthene (BbF). Key findings demonstrated that dietary lipids significantly enhanced the bioavailability of polycyclic aromatic hydrocarbons (PAHs) through synergistic active-passive transport mechanisms. Specifically, oil carriers increased BaP absorption by 14–17 % and BbF absorption by 12–15 % compared to free PAHs. Lipid-carried PAHs induced concentration-dependent cytotoxicity characterized by progressive lipid accumulation, elevated oxidative stress, mitochondrial membrane potential collapse, and apoptosis. The carrier-mediated enhancement followed a consistent potency gradient: free PAHs < OA < LNA < LA < oil carriers, implying the synergistic effect of multiple fatty acids in the oil carrier. These findings establish a pathological cycle of lipid-enhanced PAH absorption → intestinal barrier damage → further PAH accumulation, providing the first experimental analysis of dietary lipid-mediated PAH bioavailability and direct evidence for assessing PAH exposure risks in lipid-rich foods.

本研究采用Caco-2肠道模型，评估大豆油及其脂肪酸成分（油酸（OA）、亚油酸（LA）和亚麻酸（LNA））对苯并[a]芘（BaP）和苯并[b]芴（BbF）肠道吸收、转运和毒性的影响。主要研究结果表明，膳食脂类通过协同主动-被动转运机制显著提高了多环芳烃（PAHs）的生物利用度。具体来说，与游离多环芳烃相比，油载体增加了14 - 17%的BaP吸收和12 - 15%的BbF吸收。脂质携带多环芳烃诱导浓度依赖性细胞毒性，其特征为进行性脂质积累、氧化应激升高、线粒体膜电位塌陷和细胞凋亡。载体介导的增强遵循一致的效势梯度：游离PAHs <； OA < LNA < LA <；油载体，说明油载体中存在多种脂肪酸的协同作用。这些发现建立了一个脂质增强的多环芳烃吸收→肠屏障损伤→多环芳烃进一步积累的病理循环，首次提供了饮食脂质介导的多环芳烃生物利用度的实验分析，并为评估富含脂质食物中多环芳烃暴露风险提供了直接证据。

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引用次数: 0