Food Bioscience最新文献_第7页

Mizuhopecten yessoensis-derived peptide Asn-Cys-Trp alleviates lipopolysaccharide-induced intestinal barrier dysfunction mizuhopten yessosis衍生肽Asn-Cys-Trp减轻脂多糖诱导的肠屏障功能障碍

IF 5.9 1区农林科学 Q1 FOOD SCIENCE & TECHNOLOGY

Food Bioscience

Pub Date : 2026-01-12 DOI: 10.1016/j.fbio.2026.108295

Sijia Wu , Ping Jiang , Xiaoliang Zhang , Hong Zhuang

Previously, peptide Asn-Cys-Trp (NCW), derived from Mizuhopecten yessoensis, was observed to up-regulate the mRNA expression of tight junction protein ZO-1 during its transport across a Caco-2 cell monolayer. Based on this, we hypothesized that NCW could protect tight junction integrity and thereby improve intestinal barrier function. This study aimed to investigate the protective effects of NCW against LPS-induced intestinal barrier dysfunction using both in vivo and in vitro models. In lipopolysaccharide (LPS)-stimulated Caco-2 monolayer cells, NCW was found to preserve barrier function, as evidenced by attenuated decline in transepithelial electrical resistance, attenuated reductions in Claudin-1, Occludin, and ZO-1 expression, and reduced secretion of pro-inflammatory cytokines including tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), and interleukin-1β (IL-1β). In mice, oral administration of peptide NCW mitigated LPS-induced systemic and intestinal damage, as demonstrated by a lowered disease activity index and serum diamine oxidase level, alleviated jejunal and colonic histopathological damage and inflammation, and enhanced antioxidant capacity through increased colonic superoxide dismutase activity, coupled with suppressed jejunal malondialdehyde formation. Mechanistically, NCW may exert its protective effects by activating AMPK and inhibiting AKT. In conclusion, dietary peptide NCW could alleviate LPS-induced intestinal barrier injury. These findings provide novel insights into the potential application of food-derived peptides in promoting intestinal health.

在此之前，研究人员观察到来自Mizuhopecten yessoensis的肽Asn-Cys-Trp （NCW）在Caco-2细胞单层运输过程中上调紧密连接蛋白ZO-1的mRNA表达。基于此，我们假设NCW可以保护紧密连接的完整性，从而改善肠道屏障功能。本研究旨在通过体内和体外模型研究NCW对lps诱导的肠屏障功能障碍的保护作用。在脂多糖（LPS）刺激的Caco-2单层细胞中，NCW被发现可以保护屏障功能，这可以通过降低经皮上皮电阻，降低Claudin-1、Occludin和ZO-1的表达，减少促炎细胞因子包括肿瘤坏死因子-α (TNF-α)、白细胞介素-6 （IL-6）和白细胞介素-1β (IL-1β)的分泌来证明。在小鼠实验中，口服NCW肽可减轻lps诱导的全身和肠道损伤，表现为降低疾病活性指数和血清二胺氧化酶水平，减轻空肠和结肠的组织病理学损伤和炎症，并通过增加结肠超氧化物歧化酶活性增强抗氧化能力，同时抑制空肠丙二醛的形成。机制上，NCW可能通过激活AMPK和抑制AKT发挥保护作用。综上所述，饲粮中NCW肽可减轻lps诱导的肠屏障损伤。这些发现为食源性肽在促进肠道健康方面的潜在应用提供了新的见解。

{"title":"Mizuhopecten yessoensis-derived peptide Asn-Cys-Trp alleviates lipopolysaccharide-induced intestinal barrier dysfunction","authors":"Sijia Wu , Ping Jiang , Xiaoliang Zhang , Hong Zhuang","doi":"10.1016/j.fbio.2026.108295","DOIUrl":"10.1016/j.fbio.2026.108295","url":null,"abstract":"<div><div>Previously, peptide Asn-Cys-Trp (NCW), derived from <em>Mizuhopecten yessoensis</em>, was observed to up-regulate the mRNA expression of tight junction protein ZO-1 during its transport across a Caco-2 cell monolayer. Based on this, we hypothesized that NCW could protect tight junction integrity and thereby improve intestinal barrier function. This study aimed to investigate the protective effects of NCW against LPS-induced intestinal barrier dysfunction using both <em>in vivo</em> and <em>in vitro</em> models. In lipopolysaccharide (LPS)-stimulated Caco-2 monolayer cells, NCW was found to preserve barrier function, as evidenced by attenuated decline in transepithelial electrical resistance, attenuated reductions in Claudin-1, Occludin, and ZO-1 expression, and reduced secretion of pro-inflammatory cytokines including tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), and interleukin-1β (IL-1β). In mice, oral administration of peptide NCW mitigated LPS-induced systemic and intestinal damage, as demonstrated by a lowered disease activity index and serum diamine oxidase level, alleviated jejunal and colonic histopathological damage and inflammation, and enhanced antioxidant capacity through increased colonic superoxide dismutase activity, coupled with suppressed jejunal malondialdehyde formation. Mechanistically, NCW may exert its protective effects by activating AMPK and inhibiting AKT. In conclusion, dietary peptide NCW could alleviate LPS-induced intestinal barrier injury. These findings provide novel insights into the potential application of food-derived peptides in promoting intestinal health.</div></div>","PeriodicalId":12409,"journal":{"name":"Food Bioscience","volume":"76 ","pages":"Article 108295"},"PeriodicalIF":5.9,"publicationDate":"2026-01-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145974406","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Role of autophagy and pyroptosis in cadmium-induced BRL-3A hepatocyte injury and protective mechanism of quercetin 自噬和焦亡在镉致BRL-3A肝细胞损伤中的作用及槲皮素的保护机制

IF 5.9 1区农林科学 Q1 FOOD SCIENCE & TECHNOLOGY

Food Bioscience

Pub Date : 2026-01-12 DOI: 10.1016/j.fbio.2026.108287

Jicang Wang , Yinan Hu , Wenjing Yu , Hao Ling , Chengxiang Guo , Mengmeng Gao , Jing Zhu , Yue Wang , Huali Zhu

Cadmium (Cd) is a highly toxic environmental pollutant. The liver, an important metabolic organ, is particularly vulnerable to Cd toxicity. Quercetin (Que), a flavonoid with antioxidant activity, attenuates Cd-induced hepatocyte injury in rats, however its mechanism remains unclear. After BRL-3A cells were treated with Cd, Que, and rapamycin (Rapa), detection kits are used to determine the levels of specific biochemical indicators in the cells. Meanwhile, RT-qPCR is applied to assess the mRNA expression levels of target genes, and Western blot is used to measure the expression levels of target proteins. The results confirmed that Cd significantly reduced cell viability (P < 0.01). Cd induced hepatic oxidative stress by reducing the levels of superoxide dismutase (SOD), catalase (CAT), and total antioxidant capacity (T-AOC), while increasing the levels of malondialdehyde (MDA), hydrogen peroxide (H₂O₂), and glutathione - SH (GSH) (P < 0.01). Concomitantly, Cd up-regulated the mRNA and protein expression levels of the autophagy-related gene P62 and pyroptosis-related genes (NLRP3, ASC, Caspase-1, IL-1β, IL-18, and GSDMD), while down-regulating the expression levels of the autophagy-related genes ATG5, LC3, and Beclin-1 (P < 0.01). Notably, Que effectively reversed these Cd-induced cellular alterations, enhanced autophagic activity, and attenuated cell death. These findings suggest that Que can counteract Cd-induced inhibition of hepatocyte autophagy by promoting autophagy and alleviating Cd-induced pyroptosis in BRL-3A cells, thereby exerting a protective effect against Cd-induced hepatocyte injury.

镉（Cd）是一种剧毒的环境污染物。肝脏是重要的代谢器官，尤其容易受到镉中毒的伤害。槲皮素（Quercetin, Que）是一种具有抗氧化活性的类黄酮，可减轻cd诱导的大鼠肝细胞损伤，但其机制尚不清楚。BRL-3A细胞经Cd、Que和rapamycin （Rapa）处理后，使用检测试剂盒检测细胞中特定生化指标的水平。同时采用RT-qPCR评估靶基因mRNA表达水平，Western blot检测靶蛋白表达水平。结果证实，Cd显著降低了细胞活力（P < 0.01）。Cd通过降低超氧化物歧化酶（SOD）、过氧化氢酶（CAT）和总抗氧化能力（T-AOC）水平，提高丙二醛（MDA）、过氧化氢（H2O2）和谷胱甘肽-谷胱甘肽（GSH）水平诱导肝脏氧化应激（P < 0.01）。同时，Cd上调自噬相关基因P62和热噬相关基因NLRP3、ASC、Caspase-1、IL-1β、IL-18、GSDMD的mRNA和蛋白表达水平，下调自噬相关基因ATG5、LC3、Beclin-1的表达水平（P < 0.01）。值得注意的是，Que有效地逆转了这些cd诱导的细胞改变，增强了自噬活性，并减轻了细胞死亡。上述结果提示，Que可以通过促进BRL-3A细胞的自噬，减轻cd诱导的细胞焦亡，从而对抗cd诱导的肝细胞自噬抑制，从而对cd诱导的肝细胞损伤起到保护作用。

{"title":"Role of autophagy and pyroptosis in cadmium-induced BRL-3A hepatocyte injury and protective mechanism of quercetin","authors":"Jicang Wang , Yinan Hu , Wenjing Yu , Hao Ling , Chengxiang Guo , Mengmeng Gao , Jing Zhu , Yue Wang , Huali Zhu","doi":"10.1016/j.fbio.2026.108287","DOIUrl":"10.1016/j.fbio.2026.108287","url":null,"abstract":"<div><div>Cadmium (Cd) is a highly toxic environmental pollutant. The liver, an important metabolic organ, is particularly vulnerable to Cd toxicity. Quercetin (Que), a flavonoid with antioxidant activity, attenuates Cd-induced hepatocyte injury in rats, however its mechanism remains unclear. After BRL-3A cells were treated with Cd, Que, and rapamycin (Rapa), detection kits are used to determine the levels of specific biochemical indicators in the cells. Meanwhile, RT-qPCR is applied to assess the mRNA expression levels of target genes, and Western blot is used to measure the expression levels of target proteins. The results confirmed that Cd significantly reduced cell viability (<em>P</em> < 0.01). Cd induced hepatic oxidative stress by reducing the levels of superoxide dismutase (SOD), catalase (CAT), and total antioxidant capacity (T-AOC), while increasing the levels of malondialdehyde (MDA), hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>), and glutathione - SH (GSH) (<em>P</em> < 0.01). Concomitantly, Cd up-regulated the mRNA and protein expression levels of the autophagy-related gene P62 and pyroptosis-related genes (NLRP3, ASC, Caspase-1, IL-1β, IL-18, and GSDMD), while down-regulating the expression levels of the autophagy-related genes ATG5, LC3, and Beclin-1 (<em>P</em> < 0.01). Notably, Que effectively reversed these Cd-induced cellular alterations, enhanced autophagic activity, and attenuated cell death. These findings suggest that Que can counteract Cd-induced inhibition of hepatocyte autophagy by promoting autophagy and alleviating Cd-induced pyroptosis in BRL-3A cells, thereby exerting a protective effect against Cd-induced hepatocyte injury.</div></div>","PeriodicalId":12409,"journal":{"name":"Food Bioscience","volume":"76 ","pages":"Article 108287"},"PeriodicalIF":5.9,"publicationDate":"2026-01-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145973875","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Lacticaseibacillus rhamnosus SD11 improves gastrointestinal functions in multiple low doses of streptozotocin-induced type 1 diabetic mice 鼠李糖乳杆菌SD11在多次低剂量链脲佐菌素诱导的1型糖尿病小鼠中改善胃肠道功能

IF 5.9 1区农林科学 Q1 FOOD SCIENCE & TECHNOLOGY

Food Bioscience

Pub Date : 2026-01-12 DOI: 10.1016/j.fbio.2026.108290

Jongdee Nopparat , Fittree Hayeeawaema , Saranya Peerakietkhajorn , Chittipong Tipbunjong , Nawiya Huipao , Rawee Teanpaisan , Pissared Khuituan

Probiotics provide health benefits in various aspects via balancing gut microbiota homeostasis. Emerging evidence suggests that several Lactobacillus strains exhibit glucose-lowering and anti-inflammatory effects in type 1 diabetes mellitus (T1DM) models, as well as improve gastrointestinal (GI) dysfunctions. However, the systemic effects of Lacticaseibacillus rhamnosus SD11, known for oral health benefits, on hypoglycemia-related traits and diabetic constipation remain largely unexplored. In this study, we evaluated the impact of SD11 supplementation in a multiple low-dose streptozotocin (MLD-STZ)-induced diabetic mouse model (BALB/c) to determine its potential therapeutic benefits in T1DM-related metabolic and GI parameters. Mice were randomly assigned to six experimental groups: normal, normal + Lactiplantibacillus plantarum (LP), normal +SD11, DM, DM + LP, and DM + SD11. Over a 4-week intervention period, physiological parameters were monitored weekly, and blood, pancreas, kidney, and GI tissue samples were collected at endpoint. Our results indicated that supplementation with SD11 for 4 weeks significantly improved glycemic levels and mitigated diabetes-induced weight loss. These metabolic improvements were accompanied by preserved pancreatic islet morphology and an increase in β-cell mass. Moreover, SD11 enhanced GI motility, evidenced by reduced upper and total gut transit times, increased fecal water content, and higher defecation frequency. SD11 also preserved colonic mucosal integrity in diabetic mice. Collectively, these findings indicate that SD11 confers systemic benefits in a diabetic context, particularly in glycemic regulation, GI function, and the maintenance of pancreatic and mucosal architecture, highlighting its potential application for managing T1DM-associated complications.

益生菌通过平衡肠道菌群的动态平衡，在各个方面提供健康益处。新出现的证据表明，几种乳杆菌菌株在1型糖尿病（T1DM）模型中表现出降血糖和抗炎作用，并改善胃肠道（GI）功能障碍。然而，鼠李糖乳杆菌SD11的全身作用，众所周知的口腔健康益处，对低血糖相关特征和糖尿病便秘的影响在很大程度上仍未被探索。在这项研究中，我们评估了SD11补充对多重低剂量链脲佐菌素（MLD-STZ）诱导的糖尿病小鼠模型（BALB/c）的影响，以确定其在t1dm相关代谢和GI参数方面的潜在治疗益处。将小鼠随机分为正常、正常+植物乳杆菌（LP）、正常+SD11、DM、DM + LP和DM +SD11 6个实验组。在为期4周的干预期内，每周监测生理参数，并在终点收集血液、胰腺、肾脏和胃肠道组织样本。我们的研究结果表明，补充SD11 4周可显著改善血糖水平，减轻糖尿病引起的体重减轻。这些代谢改善伴随着胰岛形态的保留和β细胞质量的增加。此外，SD11增强了胃肠道运动，表现为减少了上肠和全肠运输时间，增加了粪便含水量，提高了排便频率。SD11还能保持糖尿病小鼠结肠粘膜的完整性。总之，这些研究结果表明，SD11在糖尿病患者中具有全系统益处，特别是在血糖调节、GI功能和胰腺和粘膜结构的维持方面，突出了其在治疗t1dm相关并发症方面的潜在应用。

{"title":"Lacticaseibacillus rhamnosus SD11 improves gastrointestinal functions in multiple low doses of streptozotocin-induced type 1 diabetic mice","authors":"Jongdee Nopparat , Fittree Hayeeawaema , Saranya Peerakietkhajorn , Chittipong Tipbunjong , Nawiya Huipao , Rawee Teanpaisan , Pissared Khuituan","doi":"10.1016/j.fbio.2026.108290","DOIUrl":"10.1016/j.fbio.2026.108290","url":null,"abstract":"<div><div>Probiotics provide health benefits in various aspects via balancing gut microbiota homeostasis. Emerging evidence suggests that several <em>Lactobacillus</em> strains exhibit glucose-lowering and anti-inflammatory effects in type 1 diabetes mellitus (T1DM) models, as well as improve gastrointestinal (GI) dysfunctions. However, the systemic effects of <em>Lacticaseibacillus rhamnosus</em> SD11, known for oral health benefits, on hypoglycemia-related traits and diabetic constipation remain largely unexplored. In this study, we evaluated the impact of SD11 supplementation in a multiple low-dose streptozotocin (MLD-STZ)-induced diabetic mouse model (BALB/c) to determine its potential therapeutic benefits in T1DM-related metabolic and GI parameters. Mice were randomly assigned to six experimental groups: normal, normal + <em>Lactiplantibacillus plantarum</em> (LP), normal +SD11, DM, DM + LP, and DM + SD11. Over a 4-week intervention period, physiological parameters were monitored weekly, and blood, pancreas, kidney, and GI tissue samples were collected at endpoint. Our results indicated that supplementation with SD11 for 4 weeks significantly improved glycemic levels and mitigated diabetes-induced weight loss. These metabolic improvements were accompanied by preserved pancreatic islet morphology and an increase in β-cell mass. Moreover, SD11 enhanced GI motility, evidenced by reduced upper and total gut transit times, increased fecal water content, and higher defecation frequency. SD11 also preserved colonic mucosal integrity in diabetic mice. Collectively, these findings indicate that SD11 confers systemic benefits in a diabetic context, particularly in glycemic regulation, GI function, and the maintenance of pancreatic and mucosal architecture, highlighting its potential application for managing T1DM-associated complications.</div></div>","PeriodicalId":12409,"journal":{"name":"Food Bioscience","volume":"76 ","pages":"Article 108290"},"PeriodicalIF":5.9,"publicationDate":"2026-01-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145973874","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Distribution of folate synthesis genes in Lactobacillaceae and characterization of high folate production 乳酸杆菌科叶酸合成基因的分布及高产叶酸的鉴定

IF 5.9 1区农林科学 Q1 FOOD SCIENCE & TECHNOLOGY

Food Bioscience

Pub Date : 2026-01-12 DOI: 10.1016/j.fbio.2026.108284

Zhihao Chen , Chuan Zhang , Yiyi Li , Chengcheng Zhang , Pan Huang , Gang Wang , Shumao Cui , Wenwei Lu , Bo Yang , Qixiao Zhai , Fengwei Tian

Folate is an essential vitamin that cannot be synthesized endogenously and must be obtained through the diet. Natural folate from probiotic sources is an effective method for folate supplementation. Current screening methods for high folate-producing strains, relying on time-consuming phenotypic and folate production assays, lack a rapid genomics-based strategy. Thus, we analyzed the folate synthesis pathway in 699 probiotics from 14 species to identify key genes for high folate production. Our results indicated that the integrity of the tetrahydrofolate synthesis pathway, particularly the folP genes, is essential for folate biosynthesis. High folate-producing strains possess a complete and multi-copy set of folate synthesis genes within the 6-hydroxy-7,8-dihydrofolate pyrophosphate and tetrahydrofolate biosynthesis pathways. We also found that the addition of p-aminobenzoic acid, poly-γ-glutamic acid, glutamic acid, serine, and CaCl₂ significantly enhanced folate production. Our research provides a convenient and rapid genomic screening method for identifying high folate-producing strains.

叶酸是一种必需的维生素，不能由体内合成，必须通过饮食获得。从益生菌中提取的天然叶酸是补充叶酸的有效方法。目前的高叶酸产菌株筛选方法依赖于耗时的表型和叶酸产测定，缺乏快速的基于基因组学的策略。因此，我们分析了来自14个物种的699种益生菌的叶酸合成途径，以确定高产叶酸的关键基因。我们的研究结果表明，四氢叶酸合成途径的完整性，特别是folP基因，对叶酸的生物合成至关重要。高产叶酸菌株在6-羟基-7,8-二氢叶酸焦磷酸和四氢叶酸生物合成途径中具有完整的多拷贝叶酸合成基因。我们还发现，对氨基苯甲酸、聚γ-谷氨酸、谷氨酸、丝氨酸和CaCl2的添加显著提高了叶酸的产量。本研究为鉴定高产叶酸菌株提供了一种方便、快速的基因组筛选方法。

{"title":"Distribution of folate synthesis genes in Lactobacillaceae and characterization of high folate production","authors":"Zhihao Chen , Chuan Zhang , Yiyi Li , Chengcheng Zhang , Pan Huang , Gang Wang , Shumao Cui , Wenwei Lu , Bo Yang , Qixiao Zhai , Fengwei Tian","doi":"10.1016/j.fbio.2026.108284","DOIUrl":"10.1016/j.fbio.2026.108284","url":null,"abstract":"<div><div>Folate is an essential vitamin that cannot be synthesized endogenously and must be obtained through the diet. Natural folate from probiotic sources is an effective method for folate supplementation. Current screening methods for high folate-producing strains, relying on time-consuming phenotypic and folate production assays, lack a rapid genomics-based strategy. Thus, we analyzed the folate synthesis pathway in 699 probiotics from 14 species to identify key genes for high folate production. Our results indicated that the integrity of the tetrahydrofolate synthesis pathway, particularly the <em>folP</em> genes, is essential for folate biosynthesis. High folate-producing strains possess a complete and multi-copy set of folate synthesis genes within the 6-hydroxy-7,8-dihydrofolate pyrophosphate and tetrahydrofolate biosynthesis pathways. We also found that the addition of p-aminobenzoic acid, poly-γ-glutamic acid, glutamic acid, serine, and CaCl<sub>2</sub> significantly enhanced folate production. Our research provides a convenient and rapid genomic screening method for identifying high folate-producing strains.</div></div>","PeriodicalId":12409,"journal":{"name":"Food Bioscience","volume":"76 ","pages":"Article 108284"},"PeriodicalIF":5.9,"publicationDate":"2026-01-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146034316","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of Segatella copri-accessible polysaccharides on gut microbiota and intestinal immune function of CTX-induced immunocompromised mice 龙舌兰可及多糖对ctx诱导免疫功能低下小鼠肠道菌群及肠道免疫功能的影响

IF 5.9 1区农林科学 Q1 FOOD SCIENCE & TECHNOLOGY

Food Bioscience

Pub Date : 2026-01-12 DOI: 10.1016/j.fbio.2026.108291

Yizhen Lyu , Lianghua Wen , Yadong Zhong, Dafang Yin, Mine Yang, Yanlong Wen, Yang Li, Jielun Hu

Segatella copri (formerly Prevotella copri) is a major indigestible polysaccharides consumer in the lower gut, and its interaction with polysaccharides is closely related to host health. To investigate the effects of S. copri-accessible polysaccharides with different structures (SAPS) on gut microbiota and intestinal immunity, single strain and gut microbial fermentation were performed for accessibility testing, respectively, followed by cyclophosphamide (CTX)-induced immunocompromised mice experiment. During fermentation, xylan, arabinan, arabinoxylan, and arabinogalactan (four SAPS) promoted S. copri and gut microbiota growth with increased OD₆₀₀ values, and enhanced the short-chain fatty acids (SCFAs) production, predominantly acetic acid. However, S. copri could not utilize xyloglucan. Interestingly, the four SAPS ameliorated CTX-induced immunocompromise with increased TNF-α, INF-γ, IL-6, IL-17, IL-2 in colon and index of spleen and thymus. Additionally, the four SAPS increased the relative abundance of Lactobacillus, Bacteroides, and S. copri, while inhibiting Oscillospira growth. Furthermore, they significantly promoted colonic levels of SCFAs, as well as altering the fecal metabolomic profile, e.g. Leukotriene C4, 5,6-DHET, N-Methylserotonin and 24-Hydroxycholesterol, which were involved in arachidonic acid metabolism, tryptophan metabolism, and primary bile acid biosynthesis. Notably, arabinoxylan exhibited advantages in enhancing spleen index and MUC2 expression, whereas arabinan was more effective in increasing IL-17 and IL-6. Additionally, arabinogalactan increased colonic Claudin-1 expression, and xylan showed more benefits in promoting SCFAs production. In summary, the current research explored the regulation of gut microbiota and intestinal immunity by SAPS, providing a theoretical basis for the research on the relationship among polysaccharide structure, gut microbiota, and intestinal immunity.

copri Segatella copri（原copri Prevotella）是下肠中主要的难消化多糖消费者，其与多糖的相互作用与宿主健康密切相关。为了研究不同结构的葡萄球菌可达多糖（SAPS）对肠道菌群和肠道免疫的影响，分别进行了单株和肠道微生物发酵的可达性试验，然后进行了环磷酰胺（CTX）诱导免疫功能受损小鼠实验。在发酵过程中，木聚糖、阿拉伯糖聚糖、阿拉伯木聚糖和阿拉伯半乳聚糖（4种SAPS）促进了葡萄球菌和肠道微生物群的生长，OD600值升高，并促进了短链脂肪酸（SCFAs）的产生，其中以乙酸为主。而葡萄球菌不能利用木葡聚糖。有趣的是，四种SAPS通过增加结肠中TNF-α、INF-γ、IL-6、IL-17、IL-2以及脾脏和胸腺指数来改善ctx诱导的免疫功能低下。此外，四种SAPS增加了乳杆菌、拟杆菌和葡萄球菌的相对丰度，同时抑制了示波螺旋菌的生长。此外，它们显著提高了结肠SCFAs水平，并改变了粪便代谢组学特征，如白三烯C4、5,6- dhet、n -甲基5 -羟色胺和24-羟基胆固醇，这些物质参与花生四烯酸代谢、色氨酸代谢和初级胆汁酸生物合成。值得注意的是，阿拉伯木聚糖在提高脾脏指数和MUC2表达方面具有优势，而阿拉伯木聚糖在提高IL-17和IL-6方面更为有效。此外，阿拉伯半乳聚糖增加了结肠Claudin-1的表达，木聚糖在促进SCFAs产生方面表现出更多的益处。综上所述，本研究探索了SAPS对肠道菌群和肠道免疫的调节作用，为研究多糖结构、肠道菌群和肠道免疫之间的关系提供了理论基础。

{"title":"Effects of Segatella copri-accessible polysaccharides on gut microbiota and intestinal immune function of CTX-induced immunocompromised mice","authors":"Yizhen Lyu , Lianghua Wen , Yadong Zhong, Dafang Yin, Mine Yang, Yanlong Wen, Yang Li, Jielun Hu","doi":"10.1016/j.fbio.2026.108291","DOIUrl":"10.1016/j.fbio.2026.108291","url":null,"abstract":"<div><div><em>Segatella copri</em> (formerly <em>Prevotella copri</em>) is a major indigestible polysaccharides consumer in the lower gut, and its interaction with polysaccharides is closely related to host health. To investigate the effects of <em>S. copri</em>-accessible polysaccharides with different structures (SAPS) on gut microbiota and intestinal immunity, single strain and gut microbial fermentation were performed for accessibility testing, respectively, followed by cyclophosphamide (CTX)-induced immunocompromised mice experiment. During fermentation, xylan, arabinan, arabinoxylan, and arabinogalactan (four SAPS) promoted <em>S. copri</em> and gut microbiota growth with increased OD<sub>600</sub> values, and enhanced the short-chain fatty acids (SCFAs) production, predominantly acetic acid. However, <em>S. copri</em> could not utilize xyloglucan. Interestingly, the four SAPS ameliorated CTX-induced immunocompromise with increased TNF-α, INF-γ, IL-6, IL-17, IL-2 in colon and index of spleen and thymus. Additionally, the four SAPS increased the relative abundance of <em>Lactobacillus</em>, <em>Bacteroides</em>, and <em>S. copri</em>, while inhibiting <em>Oscillospira</em> growth. Furthermore, they significantly promoted colonic levels of SCFAs, as well as altering the fecal metabolomic profile, e.g. Leukotriene C4, 5,6-DHET, N-Methylserotonin and 24-Hydroxycholesterol, which were involved in arachidonic acid metabolism, tryptophan metabolism, and primary bile acid biosynthesis. Notably, arabinoxylan exhibited advantages in enhancing spleen index and MUC2 expression, whereas arabinan was more effective in increasing IL-17 and IL-6. Additionally, arabinogalactan increased colonic Claudin-1 expression, and xylan showed more benefits in promoting SCFAs production. In summary, the current research explored the regulation of gut microbiota and intestinal immunity by SAPS, providing a theoretical basis for the research on the relationship among polysaccharide structure, gut microbiota, and intestinal immunity.</div></div>","PeriodicalId":12409,"journal":{"name":"Food Bioscience","volume":"76 ","pages":"Article 108291"},"PeriodicalIF":5.9,"publicationDate":"2026-01-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145973839","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Physicochemical properties of genistein-modified Penicillium fermentation polysaccharides and enhanced immunosuppressive activity through altered T-cell differentiation 染料木素修饰青霉菌发酵多糖的理化性质及通过改变t细胞分化增强的免疫抑制活性

IF 5.9 1区农林科学 Q1 FOOD SCIENCE & TECHNOLOGY

Food Bioscience

Pub Date : 2026-01-12 DOI: 10.1016/j.fbio.2026.108234

Kai Cheng , Gang Wang , Jianhua Xie , Zhibing Huang

Penicillium polysaccharides exhibit immunomodulatory activity, different polysaccharides produced by Penicillium EF-2 during fermentation were altered by adding different concentrations of genistein as an exogenous additive to change the fermentation environment. Structural characterization of the polysaccharides was performed and their activities were evaluated, and it was found that the Penicillium polysaccharides became more porous and their molecular weight decreased after the addition of genistein, thereby increasing their biological activity. Combined cell-based and animal studies demonstrated that genistein addition enhances the bioactivity of extracellular polysaccharides produced by Penicillium fermentation. However, for polysaccharide yield in Penicillium fermentation, polysaccharide content decreased at 3 d in both 0.5 g/L genistein (G_0.5E) and 1.5 g/L genistein (G_1.5E) treatments. Furthermore, it was concluded that polysaccharides may influence T cell differentiation by regulating energy metabolism, thereby exerting an immunosuppressive regulatory effect.

青霉菌多糖具有免疫调节活性，通过添加不同浓度的染料木素作为外源添加剂改变发酵环境，可以改变青霉菌EF-2在发酵过程中产生的不同多糖。对多糖进行了结构表征并对其活性进行了评价，发现加入染料木素后青霉菌多糖多孔性增强，分子量降低，从而提高了其生物活性。基于细胞和动物的联合研究表明，染料木素的添加提高了青霉菌发酵产生的细胞外多糖的生物活性。而对于青霉菌发酵过程中的多糖产量而言，0.5 g/L染料木素（G_0.5E）和1.5 g/L染料木素（G_1.5E）处理下的多糖含量在第3 d均有所下降。此外，多糖可能通过调节能量代谢影响T细胞分化，从而发挥免疫抑制的调节作用。

{"title":"Physicochemical properties of genistein-modified Penicillium fermentation polysaccharides and enhanced immunosuppressive activity through altered T-cell differentiation","authors":"Kai Cheng , Gang Wang , Jianhua Xie , Zhibing Huang","doi":"10.1016/j.fbio.2026.108234","DOIUrl":"10.1016/j.fbio.2026.108234","url":null,"abstract":"<div><div><em>Penicillium</em> polysaccharides exhibit immunomodulatory activity, different polysaccharides produced by <em>Penicillium</em> EF-2 during fermentation were altered by adding different concentrations of genistein as an exogenous additive to change the fermentation environment. Structural characterization of the polysaccharides was performed and their activities were evaluated, and it was found that the <em>Penicillium</em> polysaccharides became more porous and their molecular weight decreased after the addition of genistein, thereby increasing their biological activity. Combined cell-based and animal studies demonstrated that genistein addition enhances the bioactivity of extracellular polysaccharides produced by <em>Penicillium</em> fermentation. However, for polysaccharide yield in <em>Penicillium</em> fermentation, polysaccharide content decreased at 3 d in both 0.5 g/L genistein (G_0.5E) and 1.5 g/L genistein (G_1.5E) treatments. Furthermore, it was concluded that polysaccharides may influence T cell differentiation by regulating energy metabolism, thereby exerting an immunosuppressive regulatory effect.</div></div>","PeriodicalId":12409,"journal":{"name":"Food Bioscience","volume":"76 ","pages":"Article 108234"},"PeriodicalIF":5.9,"publicationDate":"2026-01-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145973864","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

PdTP1 regulates the biotransformation of limonene to α-terpineol by targeting cytochrome P450 genes in Penicillium digitatum PdTP1通过靶向指状青霉细胞色素P450基因调控柠檬烯向α-松油醇的生物转化

IF 5.9 1区农林科学 Q1 FOOD SCIENCE & TECHNOLOGY

Food Bioscience

Pub Date : 2026-01-12 DOI: 10.1016/j.fbio.2026.108276

Lulu Zhang , Jialin Huang , Yingjie Feng , Tingting Zhang , Fengyang Zheng , Mengyan Ouyang , Danyang Li , Shiying Wang , Jingwen Yang

As an important functional monoterpene, α-terpineol can be generated through biotransformation. The transcription factor PdTP1 has been previously validated for its beneficial role in α-terpineol production via limonene biotransformation in Penicillium digitatum DSM 62840. However, the mechanistic basis of PdTP1-mediated transcriptional regulation remained elusive. In this study, DNA affinity purification sequencing was employed to identify the potential target genes of PdTP1. In combination with RNA sequencing data, a total of 981 genes were identified as the direct targets of PdTP1, participating in diverse metabolic pathways. Notably, cytochrome P450 (CYP450) genes PDIDSM_08220 and PDIDSM_577403 were confirmed to be bound and activated by PdTP1 through yeast one-hybrid and dual luciferase report assays. Overexpression of PDIDSM_08220 and PDIDSM_577403 promoted limonene biotransformation and increased α-terpineol production, and opposite results were observed after the silencing of these genes. These findings indicated that CYP450 genes PDIDSM_08220 and PDIDSM_577403 were regulated by transcription factor PdTP1, and played crucial roles in the biotransformation of limonene to α-terpineol, which will help us further understand the mechanism of α-terpineol biosynthesis.

α-松油醇是一种重要的功能性单萜烯，可通过生物转化生成。转录因子PdTP1先前已被证实在指状青霉DSM 62840中通过柠檬烯生物转化产生α-松油醇的有益作用。然而，pdtp1介导的转录调控的机制基础仍不清楚。本研究采用DNA亲和纯化测序技术鉴定PdTP1的潜在靶基因。结合RNA测序数据，共鉴定出981个基因是PdTP1的直接靶点，参与多种代谢途径。值得注意的是，通过酵母单杂交和双荧光素酶报告实验证实，细胞色素P450 （CYP450）基因PDIDSM_08220和PDIDSM_577403被PdTP1结合并激活。PDIDSM_08220和PDIDSM_577403的过表达促进了柠檬烯的生物转化，增加了α-松油醇的产量，而沉默后则相反。这些结果表明，CYP450基因PDIDSM_08220和PDIDSM_577403受到转录因子PdTP1的调控，在柠檬烯向α-松油醇的生物转化过程中发挥了重要作用，这将有助于我们进一步了解α-松油醇的生物合成机制。

{"title":"PdTP1 regulates the biotransformation of limonene to α-terpineol by targeting cytochrome P450 genes in Penicillium digitatum","authors":"Lulu Zhang , Jialin Huang , Yingjie Feng , Tingting Zhang , Fengyang Zheng , Mengyan Ouyang , Danyang Li , Shiying Wang , Jingwen Yang","doi":"10.1016/j.fbio.2026.108276","DOIUrl":"10.1016/j.fbio.2026.108276","url":null,"abstract":"<div><div>As an important functional monoterpene, α-terpineol can be generated through biotransformation. The transcription factor PdTP1 has been previously validated for its beneficial role in α-terpineol production via limonene biotransformation in <em>Penicillium digitatum</em> DSM 62840. However, the mechanistic basis of PdTP1-mediated transcriptional regulation remained elusive. In this study, DNA affinity purification sequencing was employed to identify the potential target genes of PdTP1. In combination with RNA sequencing data, a total of 981 genes were identified as the direct targets of PdTP1, participating in diverse metabolic pathways. Notably, cytochrome P450 (CYP450) genes <em>PDIDSM_08220</em> and <em>PDIDSM_577403</em> were confirmed to be bound and activated by PdTP1 through yeast one-hybrid and dual luciferase report assays. Overexpression of <em>PDIDSM_08220</em> and <em>PDIDSM_577403</em> promoted limonene biotransformation and increased α-terpineol production, and opposite results were observed after the silencing of these genes. These findings indicated that CYP450 genes <em>PDIDSM_08220</em> and <em>PDIDSM_577403</em> were regulated by transcription factor PdTP1, and played crucial roles in the biotransformation of limonene to α-terpineol, which will help us further understand the mechanism of α-terpineol biosynthesis.</div></div>","PeriodicalId":12409,"journal":{"name":"Food Bioscience","volume":"76 ","pages":"Article 108276"},"PeriodicalIF":5.9,"publicationDate":"2026-01-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145973776","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ultrasound enhances the probiotic properties and anti-inflammatory function of Lacticaseibacillus rhamnosus 1.0320 by releasing functional metabolites 超声通过释放功能性代谢物增强鼠李糖乳杆菌1.0320的益生菌特性和抗炎功能

IF 5.9 1区农林科学 Q1 FOOD SCIENCE & TECHNOLOGY

Food Bioscience

Pub Date : 2026-01-12 DOI: 10.1016/j.fbio.2026.108293

Xinyu Neng , Xuefei Wu , He Zhang , Jing Zhang , Cong Xu , Jiahui Guo , Zhanmei Jiang , Juncai Hou

Postbiotics are emerging as a potential alternative strategy for regulating gut microbiota health. However, conventional inactivation methods can damage bioactive components, and the effects of ultrasonic processing parameters on postbiotic functionality remain poorly understood. This study aimed to investigate the effects of different ultrasonic parameters (400/800 W, 10–60 min) on the properties of Lacticaseibacillus rhamnosus 1.0320 and elucidate the underlying mechanism. Results indicated that complete inactivation was achieved with 400 W treatment for 60 min or 800 W treatment for 30 min. Cell damage induced by 800 W was significantly greater than that caused by 400 W (p < 0.05). Ultrasonication markedly altered bacterial surface properties, increasing hydrophobicity to 1.8 times that of viable cells, reducing self-aggregation to 11.38 %, and enhancing antioxidant capacity. The 800 W/60 min group exhibited the highest ABTS and DPPH radical scavenging rates. This group also significantly suppressed LPS-induced pro-inflammatory factors (IL-1β, TNF-α, and IL-6) while elevating anti-inflammatory factors (IL-4) (p < 0.05). Non-targeted metabolomics analysis further revealed that the destruction of cell structure triggered by the cavitation effect of ultrasound was the main reason for the functional enhancement. This study demonstrated that ultrasound is an efficient non-thermal method for producing highly active postbiotics, supporting the development of non-living anti-inflammatory microbial agents.

后生物制剂正在成为调节肠道微生物群健康的潜在替代策略。然而，传统的失活方法会破坏生物活性成分，超声波处理参数对生物后功能的影响仍然知之甚少。本研究旨在探讨不同超声参数（400/800 W, 10-60 min）对鼠李糖乳杆菌1.0320特性的影响，并探讨其作用机制。结果表明，400 W处理60 min或800 W处理30 min可达到完全失活。800 W对细胞的损伤显著大于400 W （p < 0.05）。超声能显著改变细菌的表面特性，疏水性提高到活细胞的1.8倍，自聚集性降低到11.38%，抗氧化能力增强。800 W/60 min组ABTS和DPPH自由基清除率最高。同时显著抑制lps诱导的促炎因子（IL-1β、TNF-α、IL-6），升高抗炎因子（IL-4）（p < 0.05）。非靶向代谢组学分析进一步揭示超声空化效应引发的细胞结构破坏是功能增强的主要原因。本研究表明，超声是一种高效的非热方法，用于生产高活性的后生制剂，支持非活性抗炎微生物制剂的开发。

{"title":"Ultrasound enhances the probiotic properties and anti-inflammatory function of Lacticaseibacillus rhamnosus 1.0320 by releasing functional metabolites","authors":"Xinyu Neng , Xuefei Wu , He Zhang , Jing Zhang , Cong Xu , Jiahui Guo , Zhanmei Jiang , Juncai Hou","doi":"10.1016/j.fbio.2026.108293","DOIUrl":"10.1016/j.fbio.2026.108293","url":null,"abstract":"<div><div>Postbiotics are emerging as a potential alternative strategy for regulating gut microbiota health. However, conventional inactivation methods can damage bioactive components, and the effects of ultrasonic processing parameters on postbiotic functionality remain poorly understood. This study aimed to investigate the effects of different ultrasonic parameters (400/800 W, 10–60 min) on the properties of <em>Lacticaseibacillus rhamnosus</em> 1.0320 and elucidate the underlying mechanism. Results indicated that complete inactivation was achieved with 400 W treatment for 60 min or 800 W treatment for 30 min. Cell damage induced by 800 W was significantly greater than that caused by 400 W (<em>p</em> < 0.05). Ultrasonication markedly altered bacterial surface properties, increasing hydrophobicity to 1.8 times that of viable cells, reducing self-aggregation to 11.38 %, and enhancing antioxidant capacity. The 800 W/60 min group exhibited the highest ABTS and DPPH radical scavenging rates. This group also significantly suppressed LPS-induced pro-inflammatory factors (IL-1β, TNF-α, and IL-6) while elevating anti-inflammatory factors (IL-4) (<em>p</em> < 0.05). Non-targeted metabolomics analysis further revealed that the destruction of cell structure triggered by the cavitation effect of ultrasound was the main reason for the functional enhancement. This study demonstrated that ultrasound is an efficient non-thermal method for producing highly active postbiotics, supporting the development of non-living anti-inflammatory microbial agents.</div></div>","PeriodicalId":12409,"journal":{"name":"Food Bioscience","volume":"76 ","pages":"Article 108293"},"PeriodicalIF":5.9,"publicationDate":"2026-01-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145973863","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

From traditional wet-fermented meat to modern wound -therapeutics: The transboundary journey of probiotic Lacticaseibacillus paracasei YL36 从传统的湿发酵肉到现代伤口治疗：副酪乳杆菌YL36的跨界之旅

IF 5.9 1区农林科学 Q1 FOOD SCIENCE & TECHNOLOGY

Food Bioscience

Pub Date : 2026-01-12 DOI: 10.1016/j.fbio.2026.108288

Yiwei Zuo , Kexin Deng , Wuliang Diao , Jianda Zhou

“Suanrou”, a characteristic moist fermented meat product indigenous to Southwest China, inspiring this study based on “analogical orientation” and “shape-based nourishment” concepts of traditional Chinese medicine. This study aimed to isolate lactic acid bacteria (LAB) with broad-spectrum antibacterial activity against common infectious bacteria in chronic diabetic wounds from Suanrou to promote chronic wound healing. 347 lactic acid bacteria strains were isolated from 6 types of Suanrou samples. After many screening, including antibacterial assay, hemolysis test, antibiotic susceptibility test, and aggregation assay, Lacticaseibacillus paracasei YL36 was identified as strain with optimal comprehensive performance. Subsequent analyses of whole-genome sequencing and metabolomics were conducted, along with further validation using cellular and animal models. L. paracasei YL36 exhibited inhibitory activity against five common pathogenic bacteria in chronic wounds, including Staphylococcus aureus, Klebsiella pneumoniae, Pseudomonas aeruginosa, Escherichia coli, and Streptococcus haemolyticus. Genomic analysis revealed that strain harbors gene clusters responsible for synthesizing antimicrobial metabolites such as terpenoids and class II bacteriocins; its genome length was 3,029,192 bp, with a G + C content of 46.38 %. Additionally, L. paracasei YL36 possessed rich and diverse metabolic profile, with clear associations between its metabolic pathways, metabolites, and antibacterial activity. The strain displayed excellent adhesion and colonization capabilities, with no clinically relevant antibiotic resistance genes or virulence factor genes detected in its genome, and a hemolysis rate of <5 %. L. paracasei YL36 is a safe and functionally excellent probiotic candidate strain with potential application value in the field of chronic wound healing.

“酸肉”是一种中国西南地区特有的湿发酵肉制品，启发了本研究基于中医“类比取向”和“形状营养”的概念。本研究旨在从慢性糖尿病创面中分离出对常见感染性细菌具有广谱抗菌活性的乳酸菌，促进慢性糖尿病创面愈合。从6种酸味样品中分离出347株乳酸菌。经抗菌试验、溶血试验、药敏试验、聚集试验等筛选，确定副干酪乳杆菌YL36为综合性能最佳的菌株。随后进行了全基因组测序和代谢组学分析，并使用细胞和动物模型进行了进一步验证。L. paracasei YL36对慢性伤口中常见的5种致病菌，包括金黄色葡萄球菌、肺炎克雷伯菌、铜绿假单胞菌、大肠杆菌和溶血链球菌均有抑制作用。基因组分析显示，该菌株含有负责合成萜类和II类细菌素等抗菌代谢物的基因簇；基因组长度3029192 bp， G + C含量46.38%。此外，L. paracasei YL36具有丰富多样的代谢谱，其代谢途径、代谢物与抗菌活性之间存在明确的关联。该菌株表现出良好的粘附和定植能力，其基因组中未检测到临床相关的抗生素耐药基因和毒力因子基因，溶血率为5%。副casei YL36是一种安全、功能优良的益生菌候选菌株，在慢性伤口愈合领域具有潜在的应用价值。

{"title":"From traditional wet-fermented meat to modern wound -therapeutics: The transboundary journey of probiotic Lacticaseibacillus paracasei YL36","authors":"Yiwei Zuo , Kexin Deng , Wuliang Diao , Jianda Zhou","doi":"10.1016/j.fbio.2026.108288","DOIUrl":"10.1016/j.fbio.2026.108288","url":null,"abstract":"<div><div>“Suanrou”, a characteristic moist fermented meat product indigenous to Southwest China, inspiring this study based on “analogical orientation” and “shape-based nourishment” concepts of traditional Chinese medicine. This study aimed to isolate lactic acid bacteria (LAB) with broad-spectrum antibacterial activity against common infectious bacteria in chronic diabetic wounds from Suanrou to promote chronic wound healing. 347 lactic acid bacteria strains were isolated from 6 types of Suanrou samples. After many screening, including antibacterial assay, hemolysis test, antibiotic susceptibility test, and aggregation assay, <em>Lacticaseibacillus paracasei</em> YL36 was identified as strain with optimal comprehensive performance. Subsequent analyses of whole-genome sequencing and metabolomics were conducted, along with further validation using cellular and animal models. <em>L. paracasei</em> YL36 exhibited inhibitory activity against five common pathogenic bacteria in chronic wounds, including <em>Staphylococcus aureus</em>, <em>Klebsiella pneumoniae</em>, <em>Pseudomonas aeruginosa</em>, <em>Escherichia coli</em>, and <em>Streptococcus haemolyticus</em>. Genomic analysis revealed that strain harbors gene clusters responsible for synthesizing antimicrobial metabolites such as terpenoids and class II bacteriocins; its genome length was 3,029,192 bp, with a G + C content of 46.38 %. Additionally, <em>L. paracasei</em> YL36 possessed rich and diverse metabolic profile, with clear associations between its metabolic pathways, metabolites, and antibacterial activity. The strain displayed excellent adhesion and colonization capabilities, with no clinically relevant antibiotic resistance genes or virulence factor genes detected in its genome, and a hemolysis rate of <5 %. <em>L. paracasei</em> YL36 is a safe and functionally excellent probiotic candidate strain with potential application value in the field of chronic wound healing.</div></div>","PeriodicalId":12409,"journal":{"name":"Food Bioscience","volume":"76 ","pages":"Article 108288"},"PeriodicalIF":5.9,"publicationDate":"2026-01-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145973865","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A comprehensive review on algal bioactive compounds with emphasize on its allergenicity reduction, health benefits and applications in food industry 对藻类生物活性化合物的研究进展进行了综述，重点介绍了藻类生物活性化合物的致敏性、健康益处及其在食品工业中的应用

IF 5.9 1区农林科学 Q1 FOOD SCIENCE & TECHNOLOGY

Food Bioscience

Pub Date : 2026-01-12 DOI: 10.1016/j.fbio.2026.108285

Soubhagya Tripathy , Srutee Rout , Rahel Suchintita Das , Brijesh Kumar Tiwari , Marco Garcia-Vaquero , Prem Prakash Srivastav

Algae represent a sustainable and versatile source of bioactive compounds with immense potential in nutraceutical, pharmaceutical, and functional food applications. This review comprehensively discusses the nutritional composition and therapeutic properties of algal-derived biomolecules such as proteins, polysaccharides, lipids, vitamins, minerals, phenolics, and peptides. These compounds exhibit diverse biological activities, including antioxidant, anticancer, anti-inflammatory, antidiabetic, cardioprotective, and immune-enhancing effects. Despite their benefits, algal products may pose allergenic risks from intrinsic constituents (proteins, pigments, and polysaccharides) and extrinsic sources (marine co-inhabitants). Clinical cases and analytical studies revealing allergenic mechanisms are critically examined. Furthermore, recent advancements in non-thermal and encapsulation technologies, such as cold plasma, pulsed electric fields, high-pressure processing, and microencapsulation, are highlighted as promising strategies for mitigating allergenicity while preserving bioactivity. The application of algal bioactives in bakery, dairy, and packaging industries underscores their potential to enhance nutritional quality, functionality, and sustainability. Additionally, the review emphasizes the growing role of algae in advancing the blue economy through bioresource valorization, renewable energy production, and circular food systems. Key regulatory challenges, consumer perception, and safety concerns limiting large-scale adoption are also discussed. This review provides an integrated perspective on algae as a future-oriented bioresource, addressing their health benefits, allergenicity management, and industrial utilization while identifying knowledge gaps and future research directions toward sustainable food innovation.

藻类是一种可持续和通用的生物活性化合物来源，在营养保健、制药和功能食品应用方面具有巨大的潜力。本文综述了藻类衍生生物分子的营养成分和治疗特性，如蛋白质、多糖、脂质、维生素、矿物质、酚类物质和多肽。这些化合物具有多种生物活性，包括抗氧化、抗癌、抗炎、抗糖尿病、心脏保护和免疫增强作用。尽管它们有好处，但藻类产品可能会因其内在成分（蛋白质、色素和多糖）和外在来源（海洋共生生物）而造成过敏风险。临床病例和分析研究揭示过敏机制进行严格审查。此外，最近在非热和封装技术方面的进展，如冷等离子体、脉冲电场、高压处理和微封装，被强调为在保持生物活性的同时减轻过敏原的有希望的策略。藻类生物活性物质在烘焙、乳制品和包装行业的应用强调了它们在提高营养质量、功能和可持续性方面的潜力。此外，该综述还强调了藻类在通过生物资源增值、可再生能源生产和循环粮食系统推动蓝色经济方面日益重要的作用。还讨论了主要的监管挑战、消费者认知和限制大规模采用的安全问题。本文综述了藻类作为一种面向未来的生物资源，阐述了它们的健康益处、过敏原管理和工业利用，同时确定了可持续食品创新的知识差距和未来的研究方向。

{"title":"A comprehensive review on algal bioactive compounds with emphasize on its allergenicity reduction, health benefits and applications in food industry","authors":"Soubhagya Tripathy , Srutee Rout , Rahel Suchintita Das , Brijesh Kumar Tiwari , Marco Garcia-Vaquero , Prem Prakash Srivastav","doi":"10.1016/j.fbio.2026.108285","DOIUrl":"10.1016/j.fbio.2026.108285","url":null,"abstract":"<div><div>Algae represent a sustainable and versatile source of bioactive compounds with immense potential in nutraceutical, pharmaceutical, and functional food applications. This review comprehensively discusses the nutritional composition and therapeutic properties of algal-derived biomolecules such as proteins, polysaccharides, lipids, vitamins, minerals, phenolics, and peptides. These compounds exhibit diverse biological activities, including antioxidant, anticancer, anti-inflammatory, antidiabetic, cardioprotective, and immune-enhancing effects. Despite their benefits, algal products may pose allergenic risks from intrinsic constituents (proteins, pigments, and polysaccharides) and extrinsic sources (marine co-inhabitants). Clinical cases and analytical studies revealing allergenic mechanisms are critically examined. Furthermore, recent advancements in non-thermal and encapsulation technologies, such as cold plasma, pulsed electric fields, high-pressure processing, and microencapsulation, are highlighted as promising strategies for mitigating allergenicity while preserving bioactivity. The application of algal bioactives in bakery, dairy, and packaging industries underscores their potential to enhance nutritional quality, functionality, and sustainability. Additionally, the review emphasizes the growing role of algae in advancing the blue economy through bioresource valorization, renewable energy production, and circular food systems. Key regulatory challenges, consumer perception, and safety concerns limiting large-scale adoption are also discussed. This review provides an integrated perspective on algae as a future-oriented bioresource, addressing their health benefits, allergenicity management, and industrial utilization while identifying knowledge gaps and future research directions toward sustainable food innovation.</div></div>","PeriodicalId":12409,"journal":{"name":"Food Bioscience","volume":"76 ","pages":"Article 108285"},"PeriodicalIF":5.9,"publicationDate":"2026-01-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146034855","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0