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Real-time matric potential dynamics in peat casings amended with wood fibre and bark, and their relationship to Agaricus bisporus yield and quality 木纤维和树皮改性泥炭壳的实时基质势动态及其与双孢蘑菇产量和品质的关系
IF 3 3区 生物学 Q2 MYCOLOGY Pub Date : 2025-09-19 DOI: 10.1016/j.funbio.2025.101664
E. Corbett , H. Grogan , B. McGuinness , A. Tumbure , G. Young , M.T. Gaffney
Wood fibre, uncomposted bark and windrow-composted bark were tested in two replicate experiments at amendment rates of 30, 50 and 70 % v/v with commercial peat-based mushroom casing. Their performance in terms of crop yield and mushroom quality was compared to an industry standard peat-based control. Hydro-physical behaviour (i.e. matric potential) between casing treatments was monitored during the crop by recording matric potential (Ψm) using solid-state tensiometers. Emerging and developing mushrooms utilised casing-contained water resulting in decreasing Ψm during a ‘flush’. Differences in the magnitude and duration of Ψm evolution between treatments were correlated to mushroom cropping performance and quality metrics.
Wood fibre treatments closely matched peat-based controls in water availability and crop timing, with Ψm values showing a similar rate of decrease over each flush (not exceeding −34.4 kPa). Class A mushrooms from these treatments were likewise indistinguishable from the controls in yield and quality.
Conversely, bark-based casings diverged from peat-based control treatments with their timing and water dynamics strongly influenced by management conditions such as case-run length. Increasing rates of bark addition in some instances was correlated with incrementally delayed cropping (by as much as 4 days relative to control treatments). Additionally, the comparatively open pore-structure of bark containing treatments resulted in poor water retention, overpinning (during 1st flush) and pore-structure degradation over time, requiring careful crop management. A lack of available water occurred within these casing treatments (particularly in Trial 1, flush 2), was evidenced not only by the slow development of water-stressed mushrooms (e.g. premature opening, split stipes), but was also correlated with a pattern of stalling or halted Ψm evolution, as cropping progressed. Water stress in these casing treatments ultimately led to a higher relative proportion of Class B graded mushrooms. Averaged over two trials, Class B mushrooms constituted 53 % and 47 % of the total yield for uncomposted and composted bark respectively. Comparatively peat averaged just 24 %.
In order to maintain yield, crop timing and stagger similar to that of commercial peat-based casing, peat-amended treatments must demonstrate similar expression of hydro-physical behaviour as well as an ability to retain structure over repeated flushing and watering cycles. Herein, it is demonstrated that sensor-monitored Ψm evolution coincides with substantial variation in the growth and progression of the crop and consequently in the yield of harvested mushrooms.
对木纤维、未堆肥树皮和窗台堆肥树皮进行了两次重复试验,分别以30%、50%和70% v/v的修正率对商业泥炭基蘑菇外壳进行了测试。将其在作物产量和蘑菇品质方面的表现与行业标准的泥炭基对照进行了比较。在种植过程中,通过使用固态张力计记录基质电位(Ψm),监测套管处理之间的水物理行为(即基质电位)。新生和发育中的蘑菇利用含有外壳的水,导致在“冲洗”期间Ψm减少。不同处理间Ψm进化幅度和持续时间的差异与蘑菇种植性能和品质指标相关。木纤维处理在水分供应和作物种植时间方面与以泥炭为基础的控制密切匹配,Ψm值显示每次冲洗的减少率相似(不超过- 34.4千巴)。从这些处理中得到的A类蘑菇在产量和质量上同样与对照没有区别。相反,基于树皮的套管与基于泥炭的控制处理不同,其时间和水动力学受到套管下入长度等管理条件的强烈影响。在某些情况下,树皮添加率的增加与作物种植的延迟相关(与对照处理相比延迟了4天)。此外,含有处理的树皮相对开放的孔隙结构导致保水能力差,覆盖(在第一次冲洗期间)和随着时间的推移孔隙结构退化,需要仔细的作物管理。在这些套壳处理中(特别是在试验1,冲洗2中),可用水的缺乏不仅证明了水分胁迫蘑菇的发育缓慢(例如过早开放,茎杆分裂),而且还与随着种植的进行而出现的一种停滞或停止Ψm进化模式有关。在这些套壳处理中,水分胁迫最终导致B级蘑菇的相对比例较高。平均两次试验,B类蘑菇分别占未堆肥和堆肥树皮总产量的53%和47%。相比之下,泥炭平均只有24%。为了保持与商业泥炭基套管类似的产量、作物时间和错开,泥炭改性处理必须表现出类似的水物理行为表现,以及在反复冲洗和浇水循环中保持结构的能力。本文证明,传感器监测的Ψm进化与作物生长和进展的实质性变化相一致,因此收获的蘑菇产量也发生了变化。
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引用次数: 0
Wickerhamomyces anomalus is a predator of the Castanea spp. ink disease-causing oomycetes Phytophthora cinnamomi and P. xcambivora. Based on morphological evidence, a model illustrating a specific mode of action is provided 反常Wickerhamomyces anomalus是Castanea sp . ink致病卵菌Phytophthora cinnamomi和P. xcambivora的捕食者。基于形态学证据,给出了一个说明特定作用方式的模型
IF 3 3区 生物学 Q2 MYCOLOGY Pub Date : 2025-09-19 DOI: 10.1016/j.funbio.2025.101663
Mariana Amorim-Rodrigues , Rogélio Lopes Brandão , Fernanda Cássio , Cândida Lucas
Phytophthora cinnamomi and P. xcambivora are serious phytopathogens, namely causing root rot/ink in chestnut trees, which severely threatens this historical crop. Oomycetes produce mycelium but are hard to control with traditional fungicides or BCAs. Recent research revealed Wickerhamomyces anomalus, a yeast endophyte, as a strong antagonist of key pre- and post-harvest fungal pathogens in laboratory settings. This study shows it also inhibits Phytophthora growth. Microscopy revealed that W. anomalus cells adhere to and are found inside Phytophthora hyphae, accumulating within collapsed areas, possibly nourishing on hyphal contents. These interactions occur without the intervention of volatile compounds, siderophores, or hydrolytic enzymes, leaving hyphal walls intact. SEM and TEM of hyphae from P. cinnamomi when co-cultured with W. anomalus showed numerous intrahyphal structures formed in response to the yeast-imposed stress. Otherwise, the yeast shows polarised cells, nuclei fusion, irregularly numbered spores and intercellular bridges, indicating a disrupted reproductive cycle. This is consistent with P. cinnamomi and W. anomalus recognising each other's pheromones, triggering a mating-like response leading to the yeast's attachment and internalisation without damaging the hyphal cell wall. This W. anomalus specific mode-of-action, different from the ones previously reported, suggests potential as a BCA for the pre-harvest management of Phytophthora.
栗树疫霉(Phytophthora cinnamomi)和xcambivora是严重的植物病原体,可引起栗树的根腐病,严重威胁栗树这一历史作物。卵菌产生菌丝体,但难以用传统的杀菌剂或bca控制。最近的研究表明,在实验室环境中,一种酵母内生菌Wickerhamomyces anomalus对关键的收获前和收获后真菌病原体具有很强的拮抗作用。这项研究表明,它还能抑制疫霉菌的生长。显微镜观察显示,异常W. anomalus细胞粘附在疫霉菌菌丝上,并在疫霉菌菌丝内发现,在塌陷的区域内积累,可能以菌丝内容物为食。这些相互作用在没有挥发性化合物、铁载体或水解酶的干预下发生,使菌丝壁保持完整。肉桂菌菌丝与反常W.共培养时的SEM和TEM结果显示,在酵母胁迫下,菌丝内形成了大量的菌丝结构。否则,酵母细胞极化,细胞核融合,孢子数量不规则,细胞间桥,表明生殖周期中断。这与P. cinnamomi和W. anomalus识别彼此的信息素,引发类似交配的反应相一致,导致酵母的附着和内化,而不破坏菌丝细胞壁。这种异常W. anomalus特异的作用模式,与以前报道的不同,表明了作为疫霉收获前管理的BCA的潜力。
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引用次数: 0
Molecular, morphophysiological, and pathogenic characterization of Colletotrichum isolates from strawberry plants in Santa Catarina, Brazil 巴西圣卡塔琳娜草莓炭疽菌分离株的分子、形态生理和病原学特征
IF 3 3区 生物学 Q2 MYCOLOGY Pub Date : 2025-09-18 DOI: 10.1016/j.funbio.2025.101660
Vanessa F. Soares , Aline C. Velho , Isabela M. Doria , Antonio J. Andrello , Mateus Brusco de Freitas , Marciel J. Stadnik
Strawberry (Fragaria × ananassa) holds significant importance within the small fruits group of temperate climates. However, its high susceptibility to diseases, particularly anthracnose caused by Colletotrichum isolates, can severely limit production. This study aimed to identify and characterize Colletotrichum species causing strawberry anthracnose in Santa Catarina state, Brazil. Isolates were collected from strawberry plants exhibiting typical anthracnose symptoms on different plant parts, in both organic and conventional fields. A phylogenetic analysis was conducted using six loci, including actin (ACT), β-tubulin (TUB2), calmodulin (CAL), glyceraldehyde-3-phosphate-dehydrogenase (GAPDH), glutamine synthetase (GS), and nuclear ribosomal internal transcribed spacer (ITS), to identify the Colletotrichum isolates. Morphological and cultural characteristics, including conidia size, shape, and sexual reproductive structures were assessed. Pathogenicity tests were performed on detached leaves, fruits, and whole plants of the cultivars (cvs.) Albion, Pircinque, and San Andreas. Additionally, lipolytic, lipase, and cutinase activities were evaluated. The multilocus phylogenetic analysis identified the isolates as Colletotrichum nymphaeae, Colletotrichum siamense, and Colletotrichum karstii. Six groups of colony color were observed. Colletotrichum siamense and C. karstii predominantly exhibited cylindrical conidia shapes, while most C. nymphaeae isolates were fusiform. Furthermore, the presence of sexual structures, such as asci and ascospores, was exclusively noted in C. karstii. Pathogenicity tests on leaves and fruits showed that all isolates caused leaf spots and fruit rot in the three tested cvs., except for two isolates of C. nymphaeae (M135 and M165), which did not cause leaf spots on the adaxial leaf surface of cultivar (cv.) San Andreas. Whole plant assays indicated that the isolates caused anthracnose symptoms on leaves, petioles, fruits, flowers, and crowns, with the most prominent symptoms on petioles and fruits. Colletotrichum karstii showed a three-fold higher halo diameter (10.2 mm), indicating increased lipolytic activity, while C. nymphaeae (M133) exhibited a twelve-fold higher lipase activity, and C. siamense had a twenty-eight-fold higher cutinase activity compared to controls. These findings provide new insights into the distribution and pathogenicity of Colletotrichum species causing strawberry anthracnose in Santa Catarina. The molecular, morphological, and enzymatic characterizations enhance our understanding of species-specific virulence factors, which can support the development of targeted disease management strategies for strawberry production.
草莓(Fragaria × ananassa)在温带气候的小水果群中占有重要地位。然而,它对疾病的高度易感性,特别是炭疽菌分离株引起的炭疽病,严重限制了生产。本研究旨在鉴定巴西圣卡塔琳娜州草莓炭疽病的炭疽菌种类。从有机田和常规田草莓植株不同部位表现出典型炭疽病症状的分离株中采集。利用肌动蛋白(ACT)、β-微管蛋白(TUB2)、钙调蛋白(CAL)、甘油醛-3-磷酸脱氢酶(GAPDH)、谷氨酰胺合成酶(GS)和核糖体内转录间隔酶(ITS) 6个基因座进行系统发育分析。形态学和培养特征,包括分生孢子的大小、形状和有性生殖结构进行了评估。对不同品种的离体叶片、果实和整株进行了致病性试验。阿尔比恩,皮尔辛克和圣安德烈亚斯。此外,脂溶酶,脂肪酶和角质酶活性进行了评估。多位点系统发育分析鉴定分离株为少女炭疽菌(Colletotrichum nymphaeae)、siamense炭疽菌(Colletotrichum siamense)和karstii炭疽菌(Colletotrichum)。观察6组菌落颜色。炭疽菌(Colletotrichum siamense)和卡氏炭疽菌(C. karstii)的分生孢子主要呈圆柱形,而若虫菌(C. nymphaeae)的分生孢子大部分呈梭形。此外,性结构的存在,如子囊和子囊孢子,只注意到在C. karstii。叶片和果实的致病性试验表明,所有分离株均可引起3株被试葡萄的叶斑病和果腐病。除两株株(M135和M165)未在品种叶片正面产生叶斑外,其余均未发生叶斑病。圣安德烈亚斯。全株测定结果表明,分离菌株在叶片、叶柄、果实、花和花冠上引起炭疽病症状,其中叶柄和果实的症状最突出。炭疽菌光晕直径(10.2 mm)增加了3倍,表明其脂溶酶活性增加,而C. nymphaeae (M133)的脂肪酶活性增加了12倍,C. siamense的表皮酶活性增加了28倍。这些发现为了解圣卡塔琳娜州草莓炭疽病病原菌的分布和致病性提供了新的认识。分子、形态和酶的特征增强了我们对物种特异性毒力因子的理解,这可以支持草莓生产的靶向疾病管理策略的发展。
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引用次数: 0
Role of the OTAbZIP gene in regulating growth and OTA production in Aspergillus westerdijkiae fc-1 under osmotic stress OTAbZIP基因在渗透胁迫下调控西部曲霉fc-1生长和OTA产生中的作用
IF 3 3区 生物学 Q2 MYCOLOGY Pub Date : 2025-09-15 DOI: 10.1016/j.funbio.2025.101662
Yanling Ma , Muyuan Zhuang , Tanvir Ahmad , Yuhong Yan , Weitian Yuan , Mingxuan Li , Guangyou Tan , Yingyao Deng , Yang Liu
Ochratoxin A (OTA), a potent carcinogenic mycotoxin, is produced by fungi from the Aspergillus and Penicillium genera. Reducing OTA contamination in food remains a global challenge. Osmotic stress is a key factor that influencing fungal development and secondary metabolism, particularly in Aspergillus species. In this study, we explored regulatory role of the OTAbZIP gene, a bZIP-type transcription factor, in growth and OTA biosynthesis under varying NaCl-induced osmotic conditions. Using RNA-Seq and RT-qPCR, we analyzed the transcriptomic responses of wild-type (WT) and ΔOTAbZIP mutant strains of Aspergillus westerdijkiae fc-1 exposed to 0, 20, and 100 g/L NaCl. Deletion of OTAbZIP significantly altered the expression of genes involved in DNA replication, sugar metabolism, ribosome function, and arginine and proline metabolism. The ΔOTAbZIP mutant exhibited greater sensitivity to osmotic stress and lower expression of OTA biosynthetic genes (otaA–D) than the WT. Although components of the HOG-MAPK pathway (Hog1, Gpd1, Cdc28, and Ctt1) were differentially expressed, OTAbZIP deletion did not block Hog1 activation, suggesting OTAbZIP may function in a distinct regulatory role upstream or parallel to this pathway. Overall, our findings highlight OTAbZIP as a key regulator of osmotic stress response and OTA biosynthesis in A. westerdijkiae, offering potential molecular targets to reduce OTA contamination in food and feed.
赭曲霉毒素A (OTA)是一种强致癌性真菌毒素,由曲霉属和青霉属真菌产生。减少食品中的OTA污染仍然是一项全球性挑战。渗透胁迫是影响真菌发育和次生代谢的关键因素,尤其是曲霉菌。在这项研究中,我们探索了在不同nacl诱导的渗透条件下,bzip型转录因子OTAbZIP基因在生长和OTA生物合成中的调节作用。采用RNA-Seq和RT-qPCR技术,分析了野生型(WT)和ΔOTAbZIP突变型西曲霉fc-1菌株在0、20和100 g/L NaCl下的转录组反应。OTAbZIP的缺失显著改变了DNA复制、糖代谢、核糖体功能以及精氨酸和脯氨酸代谢相关基因的表达。与WT相比,ΔOTAbZIP突变体对渗透胁迫表现出更大的敏感性,OTA生物合成基因(otaA-D)的表达量更低。尽管HOG-MAPK途径的组分(Hog1、Gpd1、Cdc28和Ctt1)的表达存在差异,但OTAbZIP的缺失并未阻断Hog1的激活,这表明OTAbZIP可能在该途径的上游或平行发挥着独特的调节作用。总之,我们的研究结果表明,OTAbZIP是西药渗透应激反应和OTA生物合成的关键调节因子,为减少食品和饲料中OTA污染提供了潜在的分子靶点。
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引用次数: 0
Around the world in eight white rot species: Assessment of enzymatic and non-enzymatic wood decay pathways of worldwide Esca Complex of Diseases (ECD) associated basidiomycetes 在世界各地的八种白腐病:评估世界范围内Esca疾病复合体(ECD)相关的子菌的酶和非酶木材腐烂途径
IF 3 3区 生物学 Q2 MYCOLOGY Pub Date : 2025-09-12 DOI: 10.1016/j.funbio.2025.101661
Alessandro Puca , Samuele Moretti , Mary-Lorène Goddard , Jacques Lalevée , Hanns-Heinz Kassemeyer , Sibylle Farine , Laura Mugnai , Christophe Bertsch
Esca Complex of Diseases (ECD) is widespread in viticultural areas worldwide, posing a serious risk to modern viticulture. The role of basidiomycetes in the onset of foliar symptoms has recently been reconsidered, highlighting a link between the reduction in these symptoms and the presence of Fomitiporia mediterranea in vines. As several basidiomycetes have been associated with wood decay in grapevine, understanding their degradation mechanisms could help unravel the role of wood degradation in ECD. Thus, a collection of ECD-associated basidiomycetes, including F. capensis, F. langloisii, F. polymorpha, F. australiensis, Tropicoporus texanus, Inonotus vitis, and Stereum hirsutum, was examined in addition to F. mediterranea. Our study investigates the production and activity of lignocellulosic enzymes (particularly class-II peroxidases and laccases), along with the low molecular weight compounds (LMWC) in the secretome of these fungi, to assess their ability to perform the Chelator-Mediated-Fenton (CMF) pathway. Results highlight the production of ligninolytic enzymes with low production of active manganese peroxidases and a virtually total absence of lignin peroxidase activity. Additionally, the study confirms that the CMF mechanism is widespread among these fungi, with all LMW secretomes fulfilling the required steps of the CMF mechanism. This study provides critical insights into the degradation strategies of ECD-associated white rot agents, offering potential new perspectives on studying ECD symptomatology and developing targeted control strategies. The findings emphasize the need to consider the CMF mechanism in the context of fungal wood degradation and its potential role in ECD development.
Esca复合病害(ECD)广泛存在于全球葡萄种植区,对现代葡萄栽培构成严重威胁。担子菌在叶面症状发作中的作用最近得到了重新考虑,强调了这些症状的减轻与葡萄藤中地中海Fomitiporia的存在之间的联系。由于几种担子菌与葡萄藤木材腐烂有关,了解它们的降解机制有助于揭示木材降解在ECD中的作用。因此,除了地中海镰刀菌外,还研究了一系列与ecd相关的担子菌,包括capensis、F. langloisii、F. polymorpha、F. australiensis、Tropicoporus texanus、Inonotus vitis和Stereum hirsutum。我们的研究调查了木质纤维素酶(特别是ii类过氧化物酶和漆酶)的产生和活性,以及这些真菌分泌组中的低分子量化合物(LMWC),以评估它们执行螯合剂介导的芬顿(CMF)途径的能力。结果强调木质素分解酶的生产具有低活性锰过氧化物酶的生产和几乎完全缺乏木质素过氧化物酶的活性。此外,该研究证实CMF机制在这些真菌中广泛存在,所有LMW分泌组都满足CMF机制所需的步骤。该研究为ECD相关白腐剂的降解策略提供了重要见解,为研究ECD症状学和制定有针对性的控制策略提供了潜在的新视角。研究结果强调需要考虑真菌木材降解背景下的CMF机制及其在ECD发展中的潜在作用。
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引用次数: 0
Penicillium rubens, a root fungal endophyte, increase stress tolerance and productivity in crops under drought stress 红青霉菌是一种根内生真菌,在干旱胁迫下提高作物的抗逆性和生产力
IF 3 3区 生物学 Q2 MYCOLOGY Pub Date : 2025-09-09 DOI: 10.1016/j.funbio.2025.101659
Cristian Atala , Ian S. Acuña-Rodríguez , Sebastián A. Reyes , Marco A. Molina–Montenegro
Mutualistic interactions with fungal endophytes are a promising strategy for enhancing plant stress tolerance and productivity. This study evaluated the impact of fungal endophytes on drought tolerance and productivity in lettuce (Lactuca sativa) and tomato (Solanum lycopersicum). Seedlings were grown under semi-controlled conditions with endophyte-treated (E+, inoculated with Penicillium rubens) and axenic (E−) plants subjected to three irrigation regimes. Productivity was measured as shoot biomass (lettuce) and fruit production (tomato). Drought tolerance was assessed via proline content, NHX1 gene expression, and root biomass, summarized by an integrated tolerance index. Inoculated lettuce showed higher tolerance, productivity, and root biomass, with no trade-off between these traits. Inoculated tomatoes exhibited higher yields under both no-stress and severe-stress conditions, with overcompensation under high stress. The integrated tolerance index highlighted enhanced productivity and tolerance in inoculated plants, demonstrating fungal endophytes as a sustainable strategy to mitigate drought stress and maintain crop yields.
与真菌内生菌的相互作用是提高植物抗逆性和生产力的一种有前途的策略。本研究评价了真菌内生菌对生菜(Lactuca sativa)和番茄(Solanum lycopersicum)耐旱性和产量的影响。幼苗在半控制条件下生长,内生菌处理(E+,接种了红青霉菌)和无菌(E−)植株在三种灌溉制度下生长。生产力以茎部生物量(生菜)和果实产量(番茄)来衡量。通过脯氨酸含量、NHX1基因表达和根系生物量进行耐旱性评价,并通过综合耐旱性指数进行总结。接种后的生菜表现出更高的耐受性、生产力和根系生物量,这些性状之间没有权衡。接种番茄在无胁迫和严重胁迫条件下均表现出较高的产量,在高胁迫条件下表现出过度补偿。综合耐受性指数强调了接种植株的生产力和耐受性提高,表明真菌内生菌是缓解干旱胁迫和保持作物产量的可持续策略。
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引用次数: 0
The wild-grown Boletus edulis (penny bun) mushroom from the granite-based substrate: Trace elements uptake and Mg, Cu, Zn, and Cd isotope fractionations 花岗岩基质中野生生长的毛囊蘑菇:微量元素吸收和Mg、Cu、Zn和Cd同位素分异
IF 3 3区 生物学 Q2 MYCOLOGY Pub Date : 2025-09-05 DOI: 10.1016/j.funbio.2025.101657
Alexandre V. Andronikov, Irina E. Andronikova, Eva Martinkova, Ondrej Sebek, Marketa Stepanova
We studied trace element distributions (with the use of the Agilent Technologies 5110 ICP-OES) and Mg, Cu, Zn, and Cd isotope fractionations (with the use of the MC-ICP-MS Neptune, ThermoFisher) in a substrate (granite-based)-to-mushroom (Boletus edulis) system. B. edulis likely intakes elements only in amounts necessary for its healthy existence, almost regardless of the composition of the substrate. Significant isotope fractionations occur at the soil-to-mushroom interface (Δ values varied from −1.58 ‰ for Mg to +0.72 ‰ for Cd). B. edulis from the granite-based substrate preferentially took up a lighter Mg isotope, whereas heavier isotopes of Cu, Zn, and Cd are taken up preferentially. Within-mushroom isotope fractionations were not so strongly pronounced. The strongest extent of the within-mushroom isotope fractionation was observed for Mg (within-mushroom Δ26Mg = −0.45 to +0.35 ‰) and Zn (within-mushroom Δ66Zn = −0.33 to +0.40 ‰) whereas the weakest, for Cu (within-mushroom Δ65Cu = −0.14 to −0.02 ‰) and Cd (within-mushroom Δ114Cd = −0.09 to +0.08 ‰). Mg and Zn isotope fractionations could be due to the physical properties of the mushroom. With no redox-related Cu isotope fractionation involved, kinetic processes and Cu+ complexation to S could lead to the observed subtle negative within-mushroom Cu isotope fractionation. Very insignificant Cd isotope fractionation can be due to still unidentified fungal-driven fractionation processes. Overall, the study conducted confirmed that B. edulis is able to uptake elements with different degrees of readiness and translocate them within the fruiting body with differing intensities subjecting the elements to isotope fractionation at different extent.
我们在基质(花岗岩基)-蘑菇(Boletus edulis)体系中研究了微量元素分布(使用Agilent Technologies 5110 ICP-OES)和Mg、Cu、Zn和Cd同位素分选(使用MC-ICP-MS Neptune, ThermoFisher)。B. edulis可能只摄入其健康生存所需的元素,几乎不考虑底物的组成。显著的同位素分异发生在土壤-蘑菇界面(Δ值从Mg的−1.58‰到Cd的+0.72‰不等)。来自花岗岩基基质的B. edulis优先吸收较轻的Mg同位素,而较重的Cu、Zn和Cd同位素优先被吸收。蘑菇内同位素分馏作用不明显。Mg(蘑菇内Δ26Mg =−0.45 ~ +0.35‰)和Zn(蘑菇内Δ66Zn =−0.33 ~ +0.40‰)的蘑菇内同位素分异程度最强,Cu(蘑菇内Δ65Cu =−0.14 ~−0.02‰)和Cd(蘑菇内Δ114Cd =−0.09 ~ +0.08‰)的蘑菇内同位素分异程度最弱。Mg和Zn同位素分馏可能是由于蘑菇的物理性质。在不涉及与氧化还原相关的Cu同位素分馏的情况下,动力学过程和Cu+与S的络合可能导致观察到的蘑菇内Cu同位素分馏的轻微负。非常微不足道的Cd同位素分馏可能是由于尚未确定的真菌驱动的分馏过程。综上所述,本研究证实了毛竹能够吸收不同准备程度的元素,并在子实体内以不同的强度进行转运,使这些元素在不同程度上发生同位素分馏。
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引用次数: 0
An exploratory study on crack healing in cementitious matrices induced by Pythium aphanidermatum 蛇皮草诱导胶凝基质裂缝愈合的探索性研究
IF 3 3区 生物学 Q2 MYCOLOGY Pub Date : 2025-09-05 DOI: 10.1016/j.funbio.2025.101658
Nahúm G. Cayo Chileno , Daniela Sales Alviano , Celuta Sales Alviano , Tatiana Cardoso e Bufalo , Talita Martins , Laércio Mesquita Júnior , Gabrielle Avelar Silva , Maria Alves Ferreira , Joaquin H. Aquino Rocha , Otavio da Fonseca Martins Gomes , Romildo Dias Toledo Filho , Saulo Rocha Ferreira
The biomineralization of calcium carbonate (CaCO3) mediated by microorganisms has been extensively studied for decades, with a predominant focus on bacteria, algae, and fungi. However, the functional and operational limitations of these species highlight the need to investigate new biological agents. This study aims to evaluate the biomineralization potential of Pythium aphanidermatum, an oomycete from the Chromista kingdom, for crack-healing applications in cement mortars. Its ureolytic capacity to accelerate CaCO3 formation was analyzed, and liquid cultures with controlled concentrations of cells and calcium acetate were designed. Additionally, the microorganism's growth in alkaline media was evaluated. A surface application technique was adapted to determine the crack repair potential in cementitious matrices. Results demonstrated that P aphanidermatum does not necessarily require urea to form CaCO3. A concentration of 105 cells/mL and 100 mM of calcium acetate were optimal for microorganism development through surface application and CaCO3 formation. It was also observed that P. aphanidermatum can tolerate alkaline environments (pH 11). Finally, its filamentous growth allowed partial filling of cracks in carbonated cement mortars. This work expands the scope of biomineralization by incorporating an organism from a previously unreported kingdom into this field, laying the foundation for sustainable and innovative applications in the construction industry.
微生物介导的碳酸钙(CaCO3)生物矿化已经被广泛研究了几十年,主要集中在细菌、藻类和真菌上。然而,这些物种的功能和操作限制突出了研究新的生物制剂的必要性。本研究旨在评估一种来自铬菌属的卵菌——蛇皮霉(Pythium aphanidermatum)在水泥砂浆中裂缝修复应用的生物矿化潜力。分析了其加速CaCO3形成的溶尿能力,并设计了控制浓度的细胞和醋酸钙的液体培养。此外,还对微生物在碱性培养基中的生长情况进行了评价。采用表面应用技术测定胶凝基质的裂纹修复潜力。结果表明,pphididermatum并不一定需要尿素来形成CaCO3。105个细胞/mL和100 mM的醋酸钙通过表面施用和CaCO3形成对微生物发育最有利。此外,还观察到假单胞菌能耐受碱性环境(pH值11)。最后,其丝状生长允许部分填充碳酸水泥砂浆的裂缝。这项工作扩大了生物矿化的范围,将一种来自以前未报道过的生物王国的生物纳入该领域,为建筑行业的可持续和创新应用奠定了基础。
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引用次数: 0
Diversity of Translucidithyrium (Mycosphaerellales, Phaeothecoidiellaceae) in China: Insights into their evolutionary split time and cophylogeny 中国透光菌属(Mycosphaerellales, phaeotheecoidiellaceae)的多样性及其进化分裂时间和共生系的研究
IF 3 3区 生物学 Q2 MYCOLOGY Pub Date : 2025-09-05 DOI: 10.1016/j.funbio.2025.101656
Jia-Yu Song , Hai-Xia Wu , Kevin D. Hyde , Wei-Feng Ding , Qiang Wang , Cui-Ling Gong , Yi-Tong Wang
The species evolution of epiphytic fungi are unique and critically important due to their association with plants. In this study, six new species of Translucidithyrium were discovered re-integrated morphological characteristics of this genus combined with phylogenetic analyses. The phylogenetic analysis revealed that all species of Translucidithyrium formed a monophyletic clade within Phaeothecoidiellaceae. The molecular clock analysis indicated that the divergence time of extant species of Translucidithyrium occurred during the Cenozoic era (18 Mya). The cophylogeny was conducted based on separately phylogenetic trees of Translucidithyrium and its host plants to explore their evolutionary relationships. Translucidithyrium's species with host plant selection likely being random. Understanding the evolutionary history of Translucidithyrium species provides new perspectives and data to support research into the evolution of epiphytic fungi.
附生真菌的物种进化是独特的和至关重要的,因为它们与植物的关系。本研究发现了6个新种,重新整合了该属的形态特征并结合系统发育分析。系统发育分析表明,所有的透光菌属属都是辉石菌科的一个单系分支。分子钟分析表明,现存Translucidithyrium的分化时间发生在新生代(18mya)。通过分别构建透光菌及其寄主植物的系统发育树,探讨二者的进化关系。带寄主植物选择可能是随机的。了解Translucidithyrium物种的进化史为研究附生真菌的进化提供了新的视角和数据。
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引用次数: 0
SNX-BAR protein StMvp1 is required for the endosomal sorting, melanin transport, and pathogenicity of Setosphaeria turcica SNX-BAR蛋白StMvp1是肝硬球菌内体分选、黑色素转运和致病性所必需的
IF 3 3区 生物学 Q2 MYCOLOGY Pub Date : 2025-09-02 DOI: 10.1016/j.funbio.2025.101653
Tianyi Zhao, Jinzhuo Shi, Lu Zhang, Wei Yuan, Ning Liu, Zhiyan Cao, Jingao Dong
The fungal pathogen Setosphaeria turcica causes northern corn leaf blight and relies on melanized appressoria for host invasion. SNX-BAR proteins are a subfamily of Sorting nexins (SNX), which regulate membrane trafficking, cargo sorting and membrane remodeling at the endosome. Previous studies have suggested that SNX-BARs play a critical role in growth, development and virulence of plant pathogens. However, its roles in S. turcica remain not fully understood. Here, we characterized the SNX-BAR protein StMvp1, a homolog of yeast Mvp1, in S. turcica and uncovered its critical role in melanin biosynthesis, autophagy, and pathogenicity. Deletion of StMvp1 impaired polarized growth, and appressorium development, significantly reducing pathogenicity on maize. StMvp1 located in endosomes and is necessary for endocytosis. Notably, ΔStMvp1 accumulated intracellular melanin due to the mislocalization of key synthases (StPKS18, StLac1, and StSCD3), which were trapped in punctate endosomal compartments. Meanwhile, ΔStMvp1 exhibited aberrant autophagosome formation and impaired autophagy-dependent appressorium maturation. Our study establishes StMvp1 as a regulator of endosomal sorting, melanin transport, and pathogenicity, providing insights into SNX-BAR-mediated pathogenesis in plant pathogenic fungi.
北方玉米叶枯病是由真菌病原菌灰斑病引起的,它依靠黑化的附着胞入侵。SNX- bar蛋白是分类连接蛋白(SNX)的一个亚家族,它调节内核体的膜运输、货物分类和膜重塑。以往的研究表明SNX-BARs在植物病原体的生长发育和毒力中起着关键作用。然而,它在S. turcica中的作用尚不完全清楚。在这里,我们鉴定了SNX-BAR蛋白StMvp1,酵母Mvp1的同源物,并揭示了其在黑色素生物合成、自噬和致病性中的关键作用。StMvp1的缺失使玉米的极化生长和附着胞发育受损,显著降低了致病性。StMvp1位于核内体中,是内吞作用所必需的。值得注意的是,ΔStMvp1由于关键合成酶(StPKS18、StLac1和StSCD3)的错误定位而积累了细胞内黑色素,这些酶被困在点状的内体室中。同时,ΔStMvp1表现出异常的自噬体形成和自噬依赖的附着胞成熟受损。我们的研究确定了StMvp1作为内体分选、黑色素运输和致病性的调节因子,为snx - bar介导的植物病原真菌发病机制提供了新的见解。
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引用次数: 0
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Fungal biology
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