In modern fruit breeding, to reduce the breeding cycle, induction of haploid plants through irradiated pollen technique is of paramount importance. However, the major drawback is the sensitivity of pollen to higher irradiation doses as it induces abnormality in double fertilization process. Hence, optimization of irradiation doses for maximum recovery of healthy seed is very important. Present work analyzed the seed developmental pattern of Citrus grandis, pollinated with gamma irradiated pollen (100-500 Gy) of C. limon, C. limetta, and C. sinensis. Not a single fruit was retained on the tree till maturity in C. grandis × C. limon crosses; however, in other two cross combinations, fruits were harvested at maturity only up to 300 Gy irradiation doses. Among the harvested fruit, normal seed decreased gradually with increasing irradiation doses and less than 5 normal seeds per fruit were obtained at 300 Gy with maximum in C. grandis × C. limetta cross. However, abnormal and empty number no. of seeds has increased significantly with increasing irradiation doses. Developmental pattern of embryo and endosperm within normal seeds was also influenced significantly by irradiation treatment. At higher doses, seeds number with both embryo and endosperm was decreased gradually and at 300 Gy, it was reduced to 59.47% as compared to control.
{"title":"Effect of gamma ray irradiated pollen technique on seed development pattern in Citrus","authors":"M. Kundu, A. Dubey","doi":"10.31742/ijgpb.80.4.11","DOIUrl":"https://doi.org/10.31742/ijgpb.80.4.11","url":null,"abstract":"In modern fruit breeding, to reduce the breeding cycle, induction of haploid plants through irradiated pollen technique is of paramount importance. However, the major drawback is the sensitivity of pollen to higher irradiation doses as it induces abnormality in double fertilization process. Hence, optimization of irradiation doses for maximum recovery of healthy seed is very important. Present work analyzed the seed developmental pattern of Citrus grandis, pollinated with gamma irradiated pollen (100-500 Gy) of C. limon, C. limetta, and C. sinensis. Not a single fruit was retained on the tree till maturity in C. grandis × C. limon crosses; however, in other two cross combinations, fruits were harvested at maturity only up to 300 Gy irradiation doses. Among the harvested fruit, normal seed decreased gradually with increasing irradiation doses and less than 5 normal seeds per fruit were obtained at 300 Gy with maximum in C. grandis × C. limetta cross. However, abnormal and empty number no. of seeds has increased significantly with increasing irradiation doses. Developmental pattern of embryo and endosperm within normal seeds was also influenced significantly by irradiation treatment. At higher doses, seeds number with both embryo and endosperm was decreased gradually and at 300 Gy, it was reduced to 59.47% as compared to control.","PeriodicalId":13321,"journal":{"name":"Indian Journal of Genetics and Plant Breeding","volume":null,"pages":null},"PeriodicalIF":1.0,"publicationDate":"2020-11-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44661936","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
G. Khosla, B. S. Gill, A. Sirari, P. Sharma, I. Dhaliwal
The variation for 100 seed weight in F2 population derived from a cross, AGS456 (an exotic vegetable type from Taiwan)/SL958 of soybean followed a normal curve with a range of 8.00-27.22g indicating quantitative nature of genetic control for seed size. Parental lines were screened with 207 SSR markers to identify polymorphism and 90 primer pairs detected polymorphism between the parents. These ninety markers were used for detecting polymorphism between two extreme bulks for seed weight. Out of these, 18 primer pairs were polymorphic for the bulks and were used for bulk segregant analysis in 200 F2 plants. One major QTL for seed weight was identified on LG M with Sat_244 and Satt175 as flanking markers, explaining 19.0 per cent phenotypic variation. Two minor QTLs were also identified on LG D1b, one in interval Satt041-Sat_069 with an estimated phenotypic variation (R2) of 6.0 per cent and the other in interval Sat_069-Sat_0183 estimating 7.0 per cent phenotypic variance. The markers flanking TLs may help in marker-assisted selection (MAS) for improvement of seed weight in soybean after fine mapping and validation.
{"title":"Identifying QTL for seed weight in a cross between vegetable and grain type soybeans","authors":"G. Khosla, B. S. Gill, A. Sirari, P. Sharma, I. Dhaliwal","doi":"10.31742/ijgpb.80.4.15","DOIUrl":"https://doi.org/10.31742/ijgpb.80.4.15","url":null,"abstract":"The variation for 100 seed weight in F2 population derived from a cross, AGS456 (an exotic vegetable type from Taiwan)/SL958 of soybean followed a normal curve with a range of 8.00-27.22g indicating quantitative nature of genetic control for seed size. Parental lines were screened with 207 SSR markers to identify polymorphism and 90 primer pairs detected polymorphism between the parents. These ninety markers were used for detecting polymorphism between two extreme bulks for seed weight. Out of these, 18 primer pairs were polymorphic for the bulks and were used for bulk segregant analysis in 200 F2 plants. One major QTL for seed weight was identified on LG M with Sat_244 and Satt175 as flanking markers, explaining 19.0 per cent phenotypic variation. Two minor QTLs were also identified on LG D1b, one in interval Satt041-Sat_069 with an estimated phenotypic variation (R2) of 6.0 per cent and the other in interval Sat_069-Sat_0183 estimating 7.0 per cent phenotypic variance. The markers flanking TLs may help in marker-assisted selection (MAS) for improvement of seed weight in soybean after fine mapping and validation.","PeriodicalId":13321,"journal":{"name":"Indian Journal of Genetics and Plant Breeding","volume":null,"pages":null},"PeriodicalIF":1.0,"publicationDate":"2020-11-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45179198","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Experiments were conducted to study the genotypic variability for tolerance to combined stresses of low availability of phosphorus (P) and drought in 14 mungbean [Vigna radiata (L.) R. Wilczek] accessions. The accessions were evaluated under four conditions viz., control (sufficient P, irrigated), low P (without P, irrigated), drought (sufficient P, withholding irrigation) and combined stresses (low P, withholding irrigation). The relative stress tolerance was estimated for 22 agro-physiological traits. The principal component analysis (PCA) and relative stress indices (RSIs) of traits exhibited significant variation among the treatments and accessions. Based on RSIs, the PCA ranking analysis showed that the accessions IC 280489, PDM 139 and IC 76491 were highly ranked and tolerant to low P, drought and combined stresses. The relative increase in component traits such as photosynthetic parameters, relative water content, above-ground biomass, seed P content and number of pods plant–1 were higher while canopy temperature and water use efficiency were reduced in tolerant accessions. In contrast, IPM 2-3 was found to be relatively sensitive to all three treatments. Tolerant accessions may be either included in the breeding program or used directly as cultivar that can be grown under low P and drought.
{"title":"Photosynthetic and yield traits identified through multivariate analysis in mungbean exhibiting tolerance to the combined stresses of low phosphorus and drought","authors":"R. Pandey, S. Meena, Gayacharan, Madanpal Singh","doi":"10.31742/IJGPB.80.3.8","DOIUrl":"https://doi.org/10.31742/IJGPB.80.3.8","url":null,"abstract":"Experiments were conducted to study the genotypic variability for tolerance to combined stresses of low availability of phosphorus (P) and drought in 14 mungbean [Vigna radiata (L.) R. Wilczek] accessions. The accessions were evaluated under four conditions viz., control (sufficient P, irrigated), low P (without P, irrigated), drought (sufficient P, withholding irrigation) and combined stresses (low P, withholding irrigation). The relative stress tolerance was estimated for 22 agro-physiological traits. The principal component analysis (PCA) and relative stress indices (RSIs) of traits exhibited significant variation among the treatments and accessions. Based on RSIs, the PCA ranking analysis showed that the accessions IC 280489, PDM 139 and IC 76491 were highly ranked and tolerant to low P, drought and combined stresses. The relative increase in component traits such as photosynthetic parameters, relative water content, above-ground biomass, seed P content and number of pods plant–1 were higher while canopy temperature and water use efficiency were reduced in tolerant accessions. In contrast, IPM 2-3 was found to be relatively sensitive to all three treatments. Tolerant accessions may be either included in the breeding program or used directly as cultivar that can be grown under low P and drought.","PeriodicalId":13321,"journal":{"name":"Indian Journal of Genetics and Plant Breeding","volume":null,"pages":null},"PeriodicalIF":1.0,"publicationDate":"2020-09-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43584214","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Harikrishna, Sunil, D. Upadhyay, Rahul Gajghate, P. Shashikumara, D. Chouhan, Swetha Singh, V. Sunilkumar, B. Manu, Nivedita Sinha, Sanjay Singh
Heat stress is one of the most limiting factors for the production of wheat. Global warming and consequent changes in climate adversely affect wheat plant growth and yield. To elucidate genetic basis and map heat tolerance traits, a set of 134 backcross inbred lines (BILs) derived from the cross between WH730/*2 HD2733 was used. The population was evaluated under late sown (LS) and very late sown (VLS) conditions, by exposing to heat stress during rabi season. Positive association of normalized difference vegetation index (NDVI), thousand grain weight (TGW), grain weight per spike (GWS), biomass and grain yield (GY) under both production conditions was observed. However, canopy temperature (CT) and days to heading (DH) showed negative correlation with GY under heat stress. A total of 9 Quantitative trait loci (QTL) were discovered on 7 chromosomes, which includes 4 QTLs in LS and 5 QTLs under VLS condition. Combining the results of these QTLs revealed a major stable QTL for DH (qDH_iari_5A) on chromosome 5A with 23% and 26% explaining phenotypic variance under both sowing conditions. QTL for NDVI was detected on chromosome 1B while QTL for SL and GY on chromosome 2A. The identified QTLs in the genomic regions could be targeted for genetic improvement and marker assisted selection for heat tolerance in wheat.
{"title":"QTL mapping for heat tolerance related traits using backcross inbred lines in wheat (Triticum aestivum L.)","authors":"Harikrishna, Sunil, D. Upadhyay, Rahul Gajghate, P. Shashikumara, D. Chouhan, Swetha Singh, V. Sunilkumar, B. Manu, Nivedita Sinha, Sanjay Singh","doi":"10.31742/IJGPB.80.3.2","DOIUrl":"https://doi.org/10.31742/IJGPB.80.3.2","url":null,"abstract":"Heat stress is one of the most limiting factors for the production of wheat. Global warming and consequent changes in climate adversely affect wheat plant growth and yield. To elucidate genetic basis and map heat tolerance traits, a set of 134 backcross inbred lines (BILs) derived from the cross between WH730/*2 HD2733 was used. The population was evaluated under late sown (LS) and very late sown (VLS) conditions, by exposing to heat stress during rabi season. Positive association of normalized difference vegetation index (NDVI), thousand grain weight (TGW), grain weight per spike (GWS), biomass and grain yield (GY) under both production conditions was observed. However, canopy temperature (CT) and days to heading (DH) showed negative correlation with GY under heat stress. A total of 9 Quantitative trait loci (QTL) were discovered on 7 chromosomes, which includes 4 QTLs in LS and 5 QTLs under VLS condition. Combining the results of these QTLs revealed a major stable QTL for DH (qDH_iari_5A) on chromosome 5A with 23% and 26% explaining phenotypic variance under both sowing conditions. QTL for NDVI was detected on chromosome 1B while QTL for SL and GY on chromosome 2A. The identified QTLs in the genomic regions could be targeted for genetic improvement and marker assisted selection for heat tolerance in wheat.","PeriodicalId":13321,"journal":{"name":"Indian Journal of Genetics and Plant Breeding","volume":null,"pages":null},"PeriodicalIF":1.0,"publicationDate":"2020-09-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88888655","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Interspecific crosses were attempted between Suraj (G. Hirsutum) and Suvin (G. Barbadense) in order to introgress fibre quality genes in elite genotypes of upland cotton. The results of 201BC1F4 individual single plant selections of 19 progenies indicated that the average values of introgression lines for all the yield components except boll number had higher values than the parental gentotypes. The ginning percentage (40.9-47.2%), boll weight (2.5-8.4g) and fibre strength (21.6-31.2 g/tex) indicated genetic improvement of the traits. BC1F4 progenies SPS 32-94, SPS 33-94, SPS 39-94, SPS 45-31, SPS 46-31, SPS 48-31, SPS 49-31 and SPS 50-31 were found to be promising for use in cotton breeding programme.
{"title":"Evaluation of breeding potential of introgression lines developed from inter-specific crossing between upland cotton (Gossypium hirsutum) and Gossypium barbadense","authors":"S. Palve, P. Mandhyan, V. N. Waghmare, N. Kate","doi":"10.31742/IJGPB.80.3.16","DOIUrl":"https://doi.org/10.31742/IJGPB.80.3.16","url":null,"abstract":"Interspecific crosses were attempted between Suraj (G. Hirsutum) and Suvin (G. Barbadense) in order to introgress fibre quality genes in elite genotypes of upland cotton. The results of 201BC1F4 individual single plant selections of 19 progenies indicated that the average values of introgression lines for all the yield components except boll number had higher values than the parental gentotypes. The ginning percentage (40.9-47.2%), boll weight (2.5-8.4g) and fibre strength (21.6-31.2 g/tex) indicated genetic improvement of the traits. BC1F4 progenies SPS 32-94, SPS 33-94, SPS 39-94, SPS 45-31, SPS 46-31, SPS 48-31, SPS 49-31 and SPS 50-31 were found to be promising for use in cotton breeding programme.","PeriodicalId":13321,"journal":{"name":"Indian Journal of Genetics and Plant Breeding","volume":null,"pages":null},"PeriodicalIF":1.0,"publicationDate":"2020-09-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89612340","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Conservation and genetic assessment of wild banana relatives is important for future breeding purposes. Haplotype network analysis was conducted to wild banana relatives comprised Ensete glaucum, Musa acuminata and Musa balbisianausing rbcL gene sequences. Sequences characterization showed high conservation level (91%), low indels (1.83%), and low parsimony informatives (3.51%). However, it was powerfull to separate the dataset at families, genera and species level; and moderately to separate at intraspecies level of wild bananas. Haplotype and nucleotide diversity of wild bananas were high. At intraspecies level, both M. acuminata and M. balbisiana showed high haplotype diversity but low nucelotide diversity among haplotypes; M. acuminata has higher value than M. balbisiana. No haplotype and nucleotide diversity in E. glaucum. Wild bananas were separated into seven haplotypes, with four haplogroups. Mutational pathway revealed that E. glaucum haplotype became root; and was closely related to M. balbisiana than M. acuminata. M. acuminata var. malaccensis haplotype became root within all M. acuminata varieties, and each haplotype differed by single point mutation.
{"title":"Haplotype network analysis of wild banana relatives Ensete glaucum, Musa acuminata and Musa balbisiana based on cpDNA rbcL sequences in ex-situ collection","authors":"L. Hapsari, D. Lestari, R. T. Probojati","doi":"10.31742/IJGPB.80.3.9","DOIUrl":"https://doi.org/10.31742/IJGPB.80.3.9","url":null,"abstract":"Conservation and genetic assessment of wild banana relatives is important for future breeding purposes. Haplotype network analysis was conducted to wild banana relatives comprised Ensete glaucum, Musa acuminata and Musa balbisianausing rbcL gene sequences. Sequences characterization showed high conservation level (91%), low indels (1.83%), and low parsimony informatives (3.51%). However, it was powerfull to separate the dataset at families, genera and species level; and moderately to separate at intraspecies level of wild bananas. Haplotype and nucleotide diversity of wild bananas were high. At intraspecies level, both M. acuminata and M. balbisiana showed high haplotype diversity but low nucelotide diversity among haplotypes; M. acuminata has higher value than M. balbisiana. No haplotype and nucleotide diversity in E. glaucum. Wild bananas were separated into seven haplotypes, with four haplogroups. Mutational pathway revealed that E. glaucum haplotype became root; and was closely related to M. balbisiana than M. acuminata. M. acuminata var. malaccensis haplotype became root within all M. acuminata varieties, and each haplotype differed by single point mutation.","PeriodicalId":13321,"journal":{"name":"Indian Journal of Genetics and Plant Breeding","volume":null,"pages":null},"PeriodicalIF":1.0,"publicationDate":"2020-09-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72683555","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Interaction of homozygous inbreds and heterozygous single, three way and double crosses with environment had shown a differential response in achieving yield stability. Seven diverse maize inbreds, their 21 single crosses and 105 each of three way and double crosses obtained through diallel were evaluated for twelve characters across three diverse locations to estimate comparative stability of homozygous and heterozygous genotypes for grain yield. Contrasts in heterobeltiosis, combining ability and stability parameters in three environments and interaction effects were observed. Gain in heterobeltiosis (%) for grain yield was observed with decreased environmental quality in different hybrid classes suggesting that heterozygous hybrids are more stable due to individual buffering in single crosses and both individual and population buffering in case of three way and double crosses. Significant increase in SCA effects was observed in moderate environment at Hyderabad rather than at high yielding environment Palem. Significant G × E and Environment (linear) in all the crosses was observed for grain yield suggesting the effect of environment and its pre dominant effect on grain yield. Stability of hybrids was attributed to their superior performance over the parents in low yielding environment. Thus the potential use of selected heterozygous hybrids would allow under diverse environments is suggested to mitigate losses arising out of climate change.
{"title":"G × E interaction studies in relation to heterosis and stability of grain yield in maize (Zea mays L.)","authors":"K. Sumalini, T. Pradeep, D. Sravani","doi":"10.31742/IJGPB.80.3.3","DOIUrl":"https://doi.org/10.31742/IJGPB.80.3.3","url":null,"abstract":"Interaction of homozygous inbreds and heterozygous single, three way and double crosses with environment had shown a differential response in achieving yield stability. Seven diverse maize inbreds, their 21 single crosses and 105 each of three way and double crosses obtained through diallel were evaluated for twelve characters across three diverse locations to estimate comparative stability of homozygous and heterozygous genotypes for grain yield. Contrasts in heterobeltiosis, combining ability and stability parameters in three environments and interaction effects were observed. Gain in heterobeltiosis (%) for grain yield was observed with decreased environmental quality in different hybrid classes suggesting that heterozygous hybrids are more stable due to individual buffering in single crosses and both individual and population buffering in case of three way and double crosses. Significant increase in SCA effects was observed in moderate environment at Hyderabad rather than at high yielding environment Palem. Significant G × E and Environment (linear) in all the crosses was observed for grain yield suggesting the effect of environment and its pre dominant effect on grain yield. Stability of hybrids was attributed to their superior performance over the parents in low yielding environment. Thus the potential use of selected heterozygous hybrids would allow under diverse environments is suggested to mitigate losses arising out of climate change.","PeriodicalId":13321,"journal":{"name":"Indian Journal of Genetics and Plant Breeding","volume":null,"pages":null},"PeriodicalIF":1.0,"publicationDate":"2020-09-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89434225","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
K. P. Viswanatha, R. Patil, H. Upadhyaya, H. Khan, S. Gururaj, Somasekhar
Groundnut (Arachis hypogaea L.) is the world’s third important source of oil. A Genomic Selection Training Population (GSTP) comprising 340 genotypes was evaluated over four locations for two seasons to study genetic variability and association among agronomical and quality traits. GSTP exhibited significant variation among the genotypes, seasons and G x E interaction. Moderate to high magnitude of genotypic coefficient of variation and phenotypic coefficient of variation coupled with high heritability was observed for most quality traits. The first 7 components of PCA analysis contributed more than 75% cumulative variability. The training population grouped into three clusters in both the seasons. The number of pods/plant, pod and seed yield/plant were significantly and positively associated with each other, while test weight had negative association with number of pods/plant and had positive association with pod and seed yield during rainy season of 2015. The significant positive correlation was also observed between oil, linoleic acid and stearic acid; similarly, linoleic acid had positive association with stearic acid and palmitic acid. Significant negative correlation was observed between oil and protein content, oleic acid and linoleic acid content. The superior genotypes, namely, ICG 5221, ICGV 01393, ICGV 07220, ICGV 97120, ICGV 06420, ICG 9507, ICGV 06188 and ICGV 00440 were best performer for yield, yield components and nutritional quality traits while ICG 2381 and ICG 5221 recorded a better performance for oil content. The rainy season was found to have an advantage for vegetative, physiological growth, oil content, oleic and oleic to linoleic ratio while the post-rainy season led to forced pod filling and reduced maturity.
{"title":"Genetic diversity, association and principle component analyses for agronomical and quality traits in genomic selection training population of groundnut (Arachis hypogaea L.)","authors":"K. P. Viswanatha, R. Patil, H. Upadhyaya, H. Khan, S. Gururaj, Somasekhar","doi":"10.31742/IJGPB.80.3.7","DOIUrl":"https://doi.org/10.31742/IJGPB.80.3.7","url":null,"abstract":"Groundnut (Arachis hypogaea L.) is the world’s third important source of oil. A Genomic Selection Training Population (GSTP) comprising 340 genotypes was evaluated over four locations for two seasons to study genetic variability and association among agronomical and quality traits. GSTP exhibited significant variation among the genotypes, seasons and G x E interaction. Moderate to high magnitude of genotypic coefficient of variation and phenotypic coefficient of variation coupled with high heritability was observed for most quality traits. The first 7 components of PCA analysis contributed more than 75% cumulative variability. The training population grouped into three clusters in both the seasons. The number of pods/plant, pod and seed yield/plant were significantly and positively associated with each other, while test weight had negative association with number of pods/plant and had positive association with pod and seed yield during rainy season of 2015. The significant positive correlation was also observed between oil, linoleic acid and stearic acid; similarly, linoleic acid had positive association with stearic acid and palmitic acid. Significant negative correlation was observed between oil and protein content, oleic acid and linoleic acid content. The superior genotypes, namely, ICG 5221, ICGV 01393, ICGV 07220, ICGV 97120, ICGV 06420, ICG 9507, ICGV 06188 and ICGV 00440 were best performer for yield, yield components and nutritional quality traits while ICG 2381 and ICG 5221 recorded a better performance for oil content. The rainy season was found to have an advantage for vegetative, physiological growth, oil content, oleic and oleic to linoleic ratio while the post-rainy season led to forced pod filling and reduced maturity.","PeriodicalId":13321,"journal":{"name":"Indian Journal of Genetics and Plant Breeding","volume":null,"pages":null},"PeriodicalIF":1.0,"publicationDate":"2020-09-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74899415","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
(Triticum aestivum) (van der Weele et al. 2000; Spollen et al. 2008, Yamaguchi and Sharp 2010; Song et al. 2016; Dalal et al. 2018). Diploid species of wheat have been used as sources of adaptive genes for various biotic and abiotic stresses (Hussien et al. 1997; Reynolds et al. 2007). However, these have not been sufficiently explored for root traits under abiotic stresses. Hence, in this study, diploid wheat species with AA, BB and DD genomes were analysed for root traits under osmotic stress conditions. Expression analysis of two genes viz. BREVIS RADIX (BRX) and, NAM/ATAF/CUC 1 (NAC1), involved in root development in Arabidopsis was also carried out to understand their regulation under osmotic stress.
(小麦)(van der Weele et al. 2000;Spollen et al. 2008, Yamaguchi and Sharp 2010;Song et al. 2016;Dalal et al. 2018)。小麦的二倍体品种已被用作适应各种生物和非生物胁迫的基因来源(Hussien et al. 1997;Reynolds et al. 2007)。然而，这些在非生物胁迫下根系性状的研究还不够充分。因此，本研究对具有AA、BB和DD基因组的二倍体小麦品种在渗透胁迫条件下根系性状进行了分析。通过对拟南芥根系发育相关基因BREVIS RADIX (BRX)和NAM/ATAF/CUC 1 (NAC1)的表达分析，了解它们在渗透胁迫下的调控作用。
{"title":"Osmotic stress induced root growth and regulation of root development related genes in progenitors of wheat","authors":"M. Dalal, S. Tiwari, Vinod","doi":"10.31742/IJGPB.80.3.14","DOIUrl":"https://doi.org/10.31742/IJGPB.80.3.14","url":null,"abstract":"(Triticum aestivum) (van der Weele et al. 2000; Spollen et al. 2008, Yamaguchi and Sharp 2010; Song et al. 2016; Dalal et al. 2018). Diploid species of wheat have been used as sources of adaptive genes for various biotic and abiotic stresses (Hussien et al. 1997; Reynolds et al. 2007). However, these have not been sufficiently explored for root traits under abiotic stresses. Hence, in this study, diploid wheat species with AA, BB and DD genomes were analysed for root traits under osmotic stress conditions. Expression analysis of two genes viz. BREVIS RADIX (BRX) and, NAM/ATAF/CUC 1 (NAC1), involved in root development in Arabidopsis was also carried out to understand their regulation under osmotic stress.","PeriodicalId":13321,"journal":{"name":"Indian Journal of Genetics and Plant Breeding","volume":null,"pages":null},"PeriodicalIF":1.0,"publicationDate":"2020-09-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77154506","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The shoot-tip explant harvested from ethyl methane sulphonate (EMS) and gamma ray (GR) mutagenized seedling was cultured over MS medium fortified with NAA and BAP for five generations to amplify the mutated sector. Mutagens reduced the regeneration efficiency of the explant and affected its plant growth regulator-dependence for multiple shoot induction. While the 12d-old shoot-tip from GR-treated seedling induced shoots with 0.5µM NAA+6.6µM BAP; that from EMS-treated seedling induced shoots with 8.8µM BAP. The present study establishes that the mutagens affect the regeneration process in the explant.
{"title":"Mutagen treatment affects the regeneration efficiency of the shoot-tip explant in Celosia cristate L. (Cockscomb)","authors":"R. S. Badere, Pallavi K. Rinkey","doi":"10.31742/IJGPB.80.3.18","DOIUrl":"https://doi.org/10.31742/IJGPB.80.3.18","url":null,"abstract":"The shoot-tip explant harvested from ethyl methane sulphonate (EMS) and gamma ray (GR) mutagenized seedling was cultured over MS medium fortified with NAA and BAP for five generations to amplify the mutated sector. Mutagens reduced the regeneration efficiency of the explant and affected its plant growth regulator-dependence for multiple shoot induction. While the 12d-old shoot-tip from GR-treated seedling induced shoots with 0.5µM NAA+6.6µM BAP; that from EMS-treated seedling induced shoots with 8.8µM BAP. The present study establishes that the mutagens affect the regeneration process in the explant.","PeriodicalId":13321,"journal":{"name":"Indian Journal of Genetics and Plant Breeding","volume":null,"pages":null},"PeriodicalIF":1.0,"publicationDate":"2020-09-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76315856","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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