Objective: In recent years, dental composite resins such as tooth-colored restoration are frequently used to restore dental cavities, coronal fractures, and congenital defects. This study aimed to evaluate the microleakage of two methacrylate-based composites (GC Kalore and Luna SDI) in class II restorations.
Materials and methods: In this experimental study, a total of 18 intact human premolars previously extracted for periodontal and orthodontic reasons were randomly divided into two groups. Similar class II cavities (box only) were prepared on all teeth and restored with two different composites. In group 1, a bonding agent (Single Bond 2-SB2; 3M ESPE) and Luna SDI composite in mesial cavities and GC Kalore composite in distal cavities were used. In group 2, Single Bond 2 and GC Kalore composite in mesial cavities and Luna SDI composite in distal cavities were applied. They were then subjected to 2000 thermal cycles in a water bath between 5-55°C (dwell time: 30 seconds in every bath and transfer time: 10 seconds). Then, they were immersed in a 2% basic fuchsin dye solution for 24 hours. After rinsing with water, they were sectioned mesiodistally and evaluated for microleakage using a stereomicroscope.
Results: Independent t-test (Mann-Whitney test) showed no statistically significant difference for microleakage in mesial and distal class II restorations between GC Kalore composite and Luna SDI composite (p = 1.000) (p= 0.852). A total of 83.4% of the Luna SDI composite samples and 66.6% of the GC Kalore composite had a microleakage score of ≤3 in class II cavities.
Conclusion: In the present study, marginal microleakage was found mainly at the gingival floor extending to 1/3 of the axial wall for the Luna SDI composite and GC Kalore composite. Furthermore, no statistically significant difference was found between the microleakage of the Class II cavities restored with Luna SDI composite and GC Kalore composite.
{"title":"Microleakage Evaluation of Two Methacrylate-Based Composites (GC Kalore and Luna SDI) in Class II Restorations: A Laboratory Study.","authors":"Kooshan Moradi, Sadaf Sadat Mahmoudinezhad, Mehran Mapar","doi":"10.1155/2022/3835694","DOIUrl":"https://doi.org/10.1155/2022/3835694","url":null,"abstract":"<p><strong>Objective: </strong>In recent years, dental composite resins such as tooth-colored restoration are frequently used to restore dental cavities, coronal fractures, and congenital defects. This study aimed to evaluate the microleakage of two methacrylate-based composites (GC Kalore and Luna SDI) in class II restorations.</p><p><strong>Materials and methods: </strong>In this experimental study, a total of 18 intact human premolars previously extracted for periodontal and orthodontic reasons were randomly divided into two groups. Similar class II cavities (box only) were prepared on all teeth and restored with two different composites. In group 1, a bonding agent (Single Bond 2-SB2; 3M ESPE) and Luna SDI composite in mesial cavities and GC Kalore composite in distal cavities were used. In group 2, Single Bond 2 and GC Kalore composite in mesial cavities and Luna SDI composite in distal cavities were applied. They were then subjected to 2000 thermal cycles in a water bath between 5-55°C (dwell time: 30 seconds in every bath and transfer time: 10 seconds). Then, they were immersed in a 2% basic fuchsin dye solution for 24 hours. After rinsing with water, they were sectioned mesiodistally and evaluated for microleakage using a stereomicroscope.</p><p><strong>Results: </strong>Independent <i>t</i>-test (Mann-Whitney test) showed no statistically significant difference for microleakage in mesial and distal class II restorations between GC Kalore composite and Luna SDI composite (<i>p</i> = 1.000) (<i>p</i>= 0.852). A total of 83.4% of the Luna SDI composite samples and 66.6% of the GC Kalore composite had a microleakage score of ≤3 in class II cavities.</p><p><strong>Conclusion: </strong>In the present study, marginal microleakage was found mainly at the gingival floor extending to 1/3 of the axial wall for the Luna SDI composite and GC Kalore composite. Furthermore, no statistically significant difference was found between the microleakage of the Class II cavities restored with Luna SDI composite and GC Kalore composite.</p>","PeriodicalId":13704,"journal":{"name":"International Journal of Biomaterials","volume":"2022 ","pages":"3835694"},"PeriodicalIF":3.1,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9812629/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10280571","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The COVID-19 pandemic has once again prompted people to resort to the remedies of folk and alternative medicine. Medicinal plants, because of their chemical composition, pharmacological properties, and the action of biologically active substances, can stop and relieve the symptoms of the disease. The purpose of the work is a comparative flora analysis of medicinal plants to identify the most prospective plant and further production of a remedy for the avoidance, treatment, and rehabilitation of COVID-19. The search for prospective medicinal plants was performed by analyzing the literature in online databases: Web of Science, Scopus, Google Scholar, and PubMed, including official WHO media sites. According to recent studies related to COVID-19, a significant number of medicinal plants with anti-inflammatory, antiviral, and immunostimulatory effects have been identified. A comparative study of nine medicinal plants was conducted to determine the most suitable medicinal plant to treat coronavirus infection. According to the results of the comparative analysis, Chamaenerion angustifolium Seg. showed itself as the most prospective medicinal plant with the greatest pharmacological effect compared with other types of medicinal plants. Its therapeutic properties allow physiological relief of 18 symptoms of coronavirus infection. It is advisable to conduct further clinical trials for the treatment and rehabilitation of COVID-19 using preparations from this plant.
新冠肺炎疫情再次促使人们求助于民间疗法和替代医学。药用植物由于其化学成分、药理特性和生物活性物质的作用,可以阻止和缓解疾病的症状。这项工作的目的是对药用植物进行比较菌群分析,以确定最有前景的植物和进一步生产用于预防、治疗和康复COVID-19的药物。通过分析在线数据库(Web of Science、Scopus、Google Scholar和PubMed)中的文献,包括世卫组织官方媒体网站,进行了潜在药用植物的搜索。根据最近与COVID-19相关的研究,已经发现了大量具有抗炎、抗病毒和免疫刺激作用的药用植物。通过对9种药用植物的比较研究,确定了最适合治疗冠状病毒感染的药用植物。根据比较分析的结果,长沙锦绣菇属植物。与其他类型的药用植物相比,其药理作用最大,是最有发展前景的药用植物。它的治疗特性可以从生理上缓解冠状病毒感染的18种症状。建议进一步开展利用该植物制剂治疗和康复COVID-19的临床试验。
{"title":"Comparative Analysis of the Efficiency of Medicinal Plants for the Treatment and Prevention of COVID-19.","authors":"Viktor Kamkin, Aidana Kamarova, Baurzhan Shalabayev, Assyltas Kussainov, Marat Anuarbekov, Serik Abeuov","doi":"10.1155/2022/5943649","DOIUrl":"https://doi.org/10.1155/2022/5943649","url":null,"abstract":"<p><p>The COVID-19 pandemic has once again prompted people to resort to the remedies of folk and alternative medicine. Medicinal plants, because of their chemical composition, pharmacological properties, and the action of biologically active substances, can stop and relieve the symptoms of the disease. The purpose of the work is a comparative flora analysis of medicinal plants to identify the most prospective plant and further production of a remedy for the avoidance, treatment, and rehabilitation of COVID-19. The search for prospective medicinal plants was performed by analyzing the literature in online databases: Web of Science, Scopus, Google Scholar, and PubMed, including official WHO media sites. According to recent studies related to COVID-19, a significant number of medicinal plants with anti-inflammatory, antiviral, and immunostimulatory effects have been identified. A comparative study of nine medicinal plants was conducted to determine the most suitable medicinal plant to treat coronavirus infection. According to the results of the comparative analysis, <i>Chamaenerion angustifolium</i> Seg. showed itself as the most prospective medicinal plant with the greatest pharmacological effect compared with other types of medicinal plants. Its therapeutic properties allow physiological relief of 18 symptoms of coronavirus infection. It is advisable to conduct further clinical trials for the treatment and rehabilitation of COVID-19 using preparations from this plant.</p>","PeriodicalId":13704,"journal":{"name":"International Journal of Biomaterials","volume":"2022 ","pages":"5943649"},"PeriodicalIF":3.1,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9759393/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10751291","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Muhammad Pandunugrahadi, Komang Agung Irianto, Oen Sindrawati
Introduction: Without appropriate treatment, nerve injuries may result in permanent loss of function. Platelet-rich plasma (PRP) injection is found to help in nerve regeneration. PRP is a concentrated platelet derived from autologous blood with the potential to release various growth factors (GF) to promote nerve regeneration. This study aims to know the best time for PRP injection to promote nerve regeneration.
Methods: This is an experimental in vivo research using male New Zealand white rabbits in the randomized control group posttest only design. Samples were divided into 5 groups (1 control group and 4 treatment groups). The control group without PRP injection and treated groups injected immediately after nerve injury, 3 days, 7 days, and 14 days afterward. Nerve regeneration was evaluated by the histology specimen sacrificed on day 21. Inflammation cells and endoneurium vacuoles were counted as mean percentage of five nerve fragments in each injured nerve sample specimen.
Result: Inflammation cells and vacuole cells increased significantly when PRP was administered 3 days after injury (group 2) (respectively, 14 ± 6.7 and 56.6 ± 11.6) compared to all treatment groups (p < 0.005) (control group, respectively, 6 ± 2.6 and 15.7 ± 9.5). On the other hand, significantly lower endoneurium vacuoles and inflammation cells were found on "the day 14" sample group (respectively, 5 ± 1.3 and 5.2 ± 1.6) compared to all other groups (p < 0.005).
Conclusion: This study found that the best time for injecting PRP for nerve regeneration is 14 days after injury.
{"title":"The Optimal Timing of Platelet-Rich Plasma (PRP) Injection for Nerve Lesion Recovery: A Preliminary Study.","authors":"Muhammad Pandunugrahadi, Komang Agung Irianto, Oen Sindrawati","doi":"10.1155/2022/9601547","DOIUrl":"https://doi.org/10.1155/2022/9601547","url":null,"abstract":"<p><strong>Introduction: </strong>Without appropriate treatment, nerve injuries may result in permanent loss of function. Platelet-rich plasma (PRP) injection is found to help in nerve regeneration. PRP is a concentrated platelet derived from autologous blood with the potential to release various growth factors (GF) to promote nerve regeneration. This study aims to know the best time for PRP injection to promote nerve regeneration.</p><p><strong>Methods: </strong>This is an experimental in vivo research using male New Zealand white rabbits in the randomized control group posttest only design. Samples were divided into 5 groups (1 control group and 4 treatment groups). The control group without PRP injection and treated groups injected immediately after nerve injury, 3 days, 7 days, and 14 days afterward. Nerve regeneration was evaluated by the histology specimen sacrificed on day 21. Inflammation cells and endoneurium vacuoles were counted as mean percentage of five nerve fragments in each injured nerve sample specimen.</p><p><strong>Result: </strong>Inflammation cells and vacuole cells increased significantly when PRP was administered 3 days after injury (group 2) (respectively, 14 ± 6.7 and 56.6 ± 11.6) compared to all treatment groups (<i>p</i> < 0.005) (control group, respectively, 6 ± 2.6 and 15.7 ± 9.5). On the other hand, significantly lower endoneurium vacuoles and inflammation cells were found on \"the day 14\" sample group (respectively, 5 ± 1.3 and 5.2 ± 1.6) compared to all other groups (<i>p</i> < 0.005).</p><p><strong>Conclusion: </strong>This study found that the best time for injecting PRP for nerve regeneration is 14 days after injury.</p>","PeriodicalId":13704,"journal":{"name":"International Journal of Biomaterials","volume":"2022 ","pages":"9601547"},"PeriodicalIF":3.1,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9106496/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10305572","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-06-29eCollection Date: 2021-01-01DOI: 10.1155/2021/8850577
Izabelle de Mello Gindri, Lucas Kuth de Azambuja, Michele da Silva Barreto, Dionatha José do Prado, Gean Vitor Salmoria, Carlos Rodrigo de Mello Roesler
The influence of the surface finishing of breast implants on physicochemical and mechanical properties, before and after extreme degradation experiments, was investigated in this study. Removal of superficial layers after degradation was verified for both smooth and rough membranes, in which local erosion was verified. FTIR results demonstrated the generation of low-molecular-weight structures in all samples due to exposure to acidic and basic environments. Furthermore, smooth samples presented higher degrees of crosslinking than rough samples. Considering the mechanical properties, no difference was verified between smooth and rough samples as received and after degradation studies. However, the pH of the degradation solution had an influence on mechanical properties of the material and a basic environment caused greater deterioration of the mechanical properties compared to acidic conditions.
{"title":"Influence of Breast Implant Surface Finishing on Physicochemical and Mechanical Properties before and after Extreme Degradation Studies.","authors":"Izabelle de Mello Gindri, Lucas Kuth de Azambuja, Michele da Silva Barreto, Dionatha José do Prado, Gean Vitor Salmoria, Carlos Rodrigo de Mello Roesler","doi":"10.1155/2021/8850577","DOIUrl":"10.1155/2021/8850577","url":null,"abstract":"<p><p>The influence of the surface finishing of breast implants on physicochemical and mechanical properties, before and after extreme degradation experiments, was investigated in this study. Removal of superficial layers after degradation was verified for both smooth and rough membranes, in which local erosion was verified. FTIR results demonstrated the generation of low-molecular-weight structures in all samples due to exposure to acidic and basic environments. Furthermore, smooth samples presented higher degrees of crosslinking than rough samples. Considering the mechanical properties, no difference was verified between smooth and rough samples as received and after degradation studies. However, the pH of the degradation solution had an influence on mechanical properties of the material and a basic environment caused greater deterioration of the mechanical properties compared to acidic conditions.</p>","PeriodicalId":13704,"journal":{"name":"International Journal of Biomaterials","volume":"2021 ","pages":"8850577"},"PeriodicalIF":3.0,"publicationDate":"2021-06-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8261180/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10436951","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-12-10eCollection Date: 2020-01-01DOI: 10.1155/2020/2630735
Bernard Owusu Asimeng, Emmanuel Nyankson, Johnson Kwame Efavi, Amartey Nii Amarkai, Gloria Pokuaa Manu, Elvis Tiburu
Magnetic Fe3O4 nanoparticles were synthesized from maize leaves and plantain peels extract mediators. Particles were characterized, and the inhibitory effects were studied on HeLa cells in vitro using cyclic voltammetry (CV). Voltammograms from the CV show that Fe3O4 NPs interaction with HeLa cells affected their electrochemical behavior. The nanoparticles formed with higher Fe3+/Fe2+ molar ratio (2.8 : 1) resulted in smaller crystallite sizes compared to those formed with lower Fe3+/Fe2+ molar ratio (1.4 : 1). The particles with the smallest crystallite size showed higher anodic peak currents, whereas the larger crystallite sizes resulted in lower anodic peak currents. The peak currents relate to cell inhibition and are confirmed by the half-maximum inhibitory concentration (IC50). The findings show that the particles have a different inhibitory mechanism on HeLa cells ion transfer and are promising to be further exploited for cancer treatment.
{"title":"Characterization and Inhibitory Effects of Magnetic Iron Oxide Nanoparticles Synthesized from Plant Extracts on HeLa Cells.","authors":"Bernard Owusu Asimeng, Emmanuel Nyankson, Johnson Kwame Efavi, Amartey Nii Amarkai, Gloria Pokuaa Manu, Elvis Tiburu","doi":"10.1155/2020/2630735","DOIUrl":"https://doi.org/10.1155/2020/2630735","url":null,"abstract":"<p><p>Magnetic Fe<sub>3</sub>O<sub>4</sub> nanoparticles were synthesized from maize leaves and plantain peels extract mediators. Particles were characterized, and the inhibitory effects were studied on HeLa cells in vitro using cyclic voltammetry (CV). Voltammograms from the CV show that Fe<sub>3</sub>O<sub>4</sub> NPs interaction with HeLa cells affected their electrochemical behavior. The nanoparticles formed with higher Fe<sup>3+</sup>/Fe<sup>2+</sup> molar ratio (2.8 : 1) resulted in smaller crystallite sizes compared to those formed with lower Fe<sup>3+</sup>/Fe<sup>2+</sup> molar ratio (1.4 : 1). The particles with the smallest crystallite size showed higher anodic peak currents, whereas the larger crystallite sizes resulted in lower anodic peak currents. The peak currents relate to cell inhibition and are confirmed by the half-maximum inhibitory concentration (IC<sub>50</sub>). The findings show that the particles have a different inhibitory mechanism on HeLa cells ion transfer and are promising to be further exploited for cancer treatment.</p>","PeriodicalId":13704,"journal":{"name":"International Journal of Biomaterials","volume":"2020 ","pages":"2630735"},"PeriodicalIF":3.1,"publicationDate":"2020-12-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2020/2630735","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38855467","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-10-01eCollection Date: 2020-01-01DOI: 10.1155/2020/8841221
Xuan Nui Pham, Hoa Thi Nguyen, Ngan Thi Pham
In recent years, the green synthesis of nanoparticles via biological processes has attracted considerable attention. Herein, we introduce a facile and green approach for the synthesis of poriferous silver nanoparticles (Ag-NPs) decorated hydroxylapatite (HAp@Ag) nanoparticles with excellent antibacterial properties. All the nanocomposites were fully characterized in the solid state via various techniques such as X-ray powder diffraction (XRD), Fourier transform infrared spectroscopy (FT-IR), scanning electron microscopy (SEM), and energy-dispersive X-ray spectrometer (EDX), in which the synthesized Ag-NPs (24 nm in diameter) and their homogeneous incorporation on HAp have been studied by ultraviolet-visible (UV-vis) technique, transmission electron microscopy (TEM), and dynamic light scattering (DLS) analysis. The obtained results indicate that the structure and morphology of HAp have no significant changes after the incorporation of Ag-NPs on its surface. Moreover, an impressive antibacterial activity of HAp@Ag nanocomposite against Gram-positive bacterium Staphylococcus aureus and Gram-negative bacteria Escherichia coli and Pseudomonas aeruginosa has been recorded by using the agar well diffusion method. As a result, the HAp@Ag nanocomposite promises to be a great biomedical material with high antibacterial properties.
近年来,通过生物过程绿色合成纳米粒子引起了广泛关注。在此,我们介绍了一种简便、绿色的方法,用于合成具有优异抗菌性能的多孔银纳米颗粒(Ag-NPs)装饰羟基磷灰石(HAp@Ag)纳米颗粒。通过 X 射线粉末衍射(XRD)、傅立叶变换红外光谱(FT-IR)、扫描电子显微镜(SEM)和能量色散 X 射线光谱仪(EDX)等多种技术,对所有纳米复合材料的固态特性进行了充分表征、其中,紫外可见光(UV-vis)技术、透射电子显微镜(TEM)和动态光散射(DLS)分析研究了合成的 Ag-NPs(直径 24 nm)及其在 HAp 上的均匀结合。结果表明,在 HAp 表面掺入 Ag-NPs 后,其结构和形态没有发生显著变化。此外,利用琼脂井扩散法记录了 HAp@Ag 纳米复合材料对革兰氏阳性菌金黄色葡萄球菌、革兰氏阴性菌大肠杆菌和绿脓杆菌的抗菌活性。因此,HAp@Ag 纳米复合材料有望成为一种具有高度抗菌性能的生物医学材料。
{"title":"Green Synthesis and Antibacterial Activity of HAp@Ag Nanocomposite Using <i>Centella asiatica</i> (L.) Urban Extract and Eggshell.","authors":"Xuan Nui Pham, Hoa Thi Nguyen, Ngan Thi Pham","doi":"10.1155/2020/8841221","DOIUrl":"10.1155/2020/8841221","url":null,"abstract":"<p><p>In recent years, the green synthesis of nanoparticles via biological processes has attracted considerable attention. Herein, we introduce a facile and green approach for the synthesis of poriferous silver nanoparticles (Ag-NPs) decorated hydroxylapatite (HAp@Ag) nanoparticles with excellent antibacterial properties. All the nanocomposites were fully characterized in the solid state via various techniques such as X-ray powder diffraction (XRD), Fourier transform infrared spectroscopy (FT-IR), scanning electron microscopy (SEM), and energy-dispersive X-ray spectrometer (EDX), in which the synthesized Ag-NPs (24 nm in diameter) and their homogeneous incorporation on HAp have been studied by ultraviolet-visible (UV-vis) technique, transmission electron microscopy (TEM), and dynamic light scattering (DLS) analysis. The obtained results indicate that the structure and morphology of HAp have no significant changes after the incorporation of Ag-NPs on its surface. Moreover, an impressive antibacterial activity of HAp@Ag nanocomposite against Gram-positive bacterium <i>Staphylococcus aureus</i> and Gram-negative bacteria <i>Escherichia coli</i> and <i>Pseudomonas aeruginosa</i> has been recorded by using the agar well diffusion method. As a result, the HAp@Ag nanocomposite promises to be a great biomedical material with high antibacterial properties.</p>","PeriodicalId":13704,"journal":{"name":"International Journal of Biomaterials","volume":"2020 ","pages":"8841221"},"PeriodicalIF":3.1,"publicationDate":"2020-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7547362/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38494281","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-09-29eCollection Date: 2020-01-01DOI: 10.1155/2020/9801420
G Pedrazzi, C Galli, M Mattioli-Belmonte, S Guizzardi
{"title":"Response to: Comment on \"The Use of Pulsed Electromagnetic Fields to Promote Bone Responses to Biomaterials In Vitro and In Vivo\".","authors":"G Pedrazzi, C Galli, M Mattioli-Belmonte, S Guizzardi","doi":"10.1155/2020/9801420","DOIUrl":"https://doi.org/10.1155/2020/9801420","url":null,"abstract":"<p />","PeriodicalId":13704,"journal":{"name":"International Journal of Biomaterials","volume":"2020 ","pages":"9801420"},"PeriodicalIF":3.1,"publicationDate":"2020-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2020/9801420","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38494282","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-09-14eCollection Date: 2020-01-01DOI: 10.1155/2020/4158086
Samira R Akaji, David Dewez
Functionalized glutathione on chitosan-genipin cross-linked beads (CS-GG) was synthesized and tested as an adsorbent for the removal of Fe(II) and Cu(II) from aqueous solution. The beads were characterized by several techniques, including Fourier-transform infrared spectroscopy (FTIR), thermogravimetric analysis (TGA), CNS elementary analysis, scanning electron microscopy (SEM), and atomic force microscopy (AFM). The effect of several parameters such as the pH, the temperature, and the contact time was tested to optimize the condition for the adsorption reaction. The beads were incubated in aqueous solutions contaminated with different concentrations of Fe(II) and Cu(II) (under the range concentration from 10 to 400 mg·L-1), and the adsorption capacity was evaluated by inductively coupled plasma optical emission spectrometry (ICP-OES). The adsorption equilibrium was reached after 120 min of incubation under optimal pH 5 for Fe(II) and after 180 min under optimal pH 6 for Cu(II). According to the Langmuir isotherm, the maximum adsorption capacities (qmax) for Fe(II) and Cu(II) were 208 mg·g-1 and 217 mg·g-1, respectively. Our results showed that the adsorption efficiency of both metals on CS-GG beads was correlated with the degree of temperature. In addition, the adsorption reaction was spontaneous and endothermic, indicated by the positive values of ΔG0 and ΔH0. Therefore, the present study demonstrated that the new synthesized CS-GG beads had a strong adsorption capacity for Fe(II) and Cu(II) and were efficient to remove these trace metals from aqueous solution.
{"title":"Functionalized Glutathione on Chitosan-Genipin Cross-Linked Beads Used for the Removal of Trace Metals from Water.","authors":"Samira R Akaji, David Dewez","doi":"10.1155/2020/4158086","DOIUrl":"10.1155/2020/4158086","url":null,"abstract":"<p><p>Functionalized glutathione on chitosan-genipin cross-linked beads (CS-GG) was synthesized and tested as an adsorbent for the removal of Fe(II) and Cu(II) from aqueous solution. The beads were characterized by several techniques, including Fourier-transform infrared spectroscopy (FTIR), thermogravimetric analysis (TGA), CNS elementary analysis, scanning electron microscopy (SEM), and atomic force microscopy (AFM). The effect of several parameters such as the pH, the temperature, and the contact time was tested to optimize the condition for the adsorption reaction. The beads were incubated in aqueous solutions contaminated with different concentrations of Fe(II) and Cu(II) (under the range concentration from 10 to 400 mg·L<sup>-1</sup>), and the adsorption capacity was evaluated by inductively coupled plasma optical emission spectrometry (ICP-OES). The adsorption equilibrium was reached after 120 min of incubation under optimal pH 5 for Fe(II) and after 180 min under optimal pH 6 for Cu(II). According to the <i>Langmuir</i> isotherm, the maximum adsorption capacities (<i>q</i> <sub>max</sub>) for Fe(II) and Cu(II) were 208 mg·g<sup>-1</sup> and 217 mg·g<sup>-1</sup>, respectively. Our results showed that the adsorption efficiency of both metals on CS-GG beads was correlated with the degree of temperature. In addition, the adsorption reaction was spontaneous and endothermic, indicated by the positive values of Δ<i>G</i> <sup>0</sup> and Δ<i>H</i> <sup>0</sup>. Therefore, the present study demonstrated that the new synthesized CS-GG beads had a strong adsorption capacity for Fe(II) and Cu(II) and were efficient to remove these trace metals from aqueous solution.</p>","PeriodicalId":13704,"journal":{"name":"International Journal of Biomaterials","volume":"2020 ","pages":"4158086"},"PeriodicalIF":3.1,"publicationDate":"2020-09-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7509577/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38445672","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A novel natural hydroxyapatite (HAp) bioceramic was extracted from the ostrich cortical bone by the thermal decomposition method. HAp was characterized by different analytical tools such as thermogravimetric analysis (TGA), Fourier-transform infrared spectroscopy (FTIR), X-ray diffraction (XRD) analysis, and scanning electron microscopy (SEM). Removal of organic impurities from the bone powder was confirmed by TGA analysis. FTIR spectra of HAp confirmed the presence of the major functional groups such as phosphate (PO43-), hydroxyl (OH-), and carbonate (CO32-) in the bioceramic. The XRD data revealed that the HAp was the crystalline phase obtained by calcination of the bone powder at 950°C, and the SEM analyses confirmed the typical plate-like texture of the nanosized HAp crystals.
{"title":"Extraction and Characterization of Novel Natural Hydroxyapatite Bioceramic by Thermal Decomposition of Waste Ostrich Bone.","authors":"Komal Prasad Malla, Sagar Regmi, Achyut Nepal, Sitaram Bhattarai, Ram Jeewan Yadav, Shinichi Sakurai, Rameshwar Adhikari","doi":"10.1155/2020/1690178","DOIUrl":"https://doi.org/10.1155/2020/1690178","url":null,"abstract":"<p><p>A novel natural hydroxyapatite (HAp) bioceramic was extracted from the ostrich cortical bone by the thermal decomposition method. HAp was characterized by different analytical tools such as thermogravimetric analysis (TGA), Fourier-transform infrared spectroscopy (FTIR), X-ray diffraction (XRD) analysis, and scanning electron microscopy (SEM). Removal of organic impurities from the bone powder was confirmed by TGA analysis. FTIR spectra of HAp confirmed the presence of the major functional groups such as phosphate (PO<sub>4</sub> <sup>3-</sup>), hydroxyl (OH<sup>-</sup>), and carbonate (CO<sub>3</sub> <sup>2-</sup>) in the bioceramic. The XRD data revealed that the HAp was the crystalline phase obtained by calcination of the bone powder at 950°C, and the SEM analyses confirmed the typical plate-like texture of the nanosized HAp crystals.</p>","PeriodicalId":13704,"journal":{"name":"International Journal of Biomaterials","volume":"2020 ","pages":"1690178"},"PeriodicalIF":3.1,"publicationDate":"2020-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2020/1690178","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38364079","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-08-21eCollection Date: 2020-01-01DOI: 10.1155/2020/3637098
Jeimmy González-Masís, Jorge M Cubero-Sesin, Yendry Regina Corrales-Ureña, Sara González-Camacho, Nohelia Mora-Ugalde, José Roberto Vega-Baudrit, Klaus Rischka, Virendra Verma, Rodolfo J Gonzalez-Paz
Tinospora cordifolia extract contains antioxidants such as polyphenols, and thus, it has been used as a natural phytochemical antioxidant therapeutic agent. Many of these compounds are insoluble or only partially soluble in water. In this study, we produced a novel aqueous nanoparticle formulation, with an average particle size of 182.9 ± 3.8 nm, to improve the dispersion of the bioactive compounds in water and to increment its bioavailability. The nanoparticles are composed of polyphenols, alkaloids, and glycosides. We studied the effect of this nanoparticle formulation on mouse 3T3 fibroblast cell viability and New Zealand rabbit dermal irritability tests. Concentrations of 2.5, 25, and 250 µg/mL resulted in similar cell viability to cells in culture media. An intermediate concentration of 12.45 mg/ml was used for the acute dermal irritability test. There were no severe alterations that compromised animal health. These results represent a precedent for application of such nanoparticles derived from plant stems, such as Tinospora cordifolia, in biomedicine and in antiaging cosmetic treatments.
{"title":"Nonirritant and Cytocompatible <i>Tinospora cordifolia</i> Nanoparticles for Topical Antioxidant Treatments.","authors":"Jeimmy González-Masís, Jorge M Cubero-Sesin, Yendry Regina Corrales-Ureña, Sara González-Camacho, Nohelia Mora-Ugalde, José Roberto Vega-Baudrit, Klaus Rischka, Virendra Verma, Rodolfo J Gonzalez-Paz","doi":"10.1155/2020/3637098","DOIUrl":"10.1155/2020/3637098","url":null,"abstract":"<p><p><i>Tinospora cordifolia</i> extract contains antioxidants such as polyphenols, and thus, it has been used as a natural phytochemical antioxidant therapeutic agent. Many of these compounds are insoluble or only partially soluble in water. In this study, we produced a novel aqueous nanoparticle formulation, with an average particle size of 182.9 ± 3.8 nm, to improve the dispersion of the bioactive compounds in water and to increment its bioavailability. The nanoparticles are composed of polyphenols, alkaloids, and glycosides. We studied the effect of this nanoparticle formulation on mouse 3T3 fibroblast cell viability and New Zealand rabbit dermal irritability tests. Concentrations of 2.5, 25, and 250 <i>µ</i>g/mL resulted in similar cell viability to cells in culture media. An intermediate concentration of 12.45 mg/ml was used for the acute dermal irritability test. There were no severe alterations that compromised animal health. These results represent a precedent for application of such nanoparticles derived from plant stems, such as <i>Tinospora cordifolia</i>, in biomedicine and in antiaging cosmetic treatments.</p>","PeriodicalId":13704,"journal":{"name":"International Journal of Biomaterials","volume":"2020 ","pages":"3637098"},"PeriodicalIF":3.0,"publicationDate":"2020-08-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7456473/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38359232","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}