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Molecular Identification of Hydrocarbon Degrading Bacteria Isolated from Contaminated Soil of Automobile Mechanic Workshop in Lapai, Niger State 尼日尔州拉派市汽车机械车间污染土壤中烃类降解菌的分子鉴定
Pub Date : 2019-10-05 DOI: 10.18782/2320-7051.7588
A. Hassana
Hydrocarbon contamination is one of the central environmental problems today as a result of petrochemical industries’ related activities. Petroleum hydrocarbons are the organic contaminants of key interest because of their complex structure, wide spread, toxicity and persistence. Accumulation of these pollutants in plants and animal tissues has been reported to cause death or mutation. Hence this study focused on molecular identification of hydrocarbon degrading bacteria isolated from hydrocarbon contaminated soil of automobile workshop in Lapai, Niger State, Nigeria. Isolation was carried out on Mineral Salt Media (MSM) containing 1% used engine oil as carbon source. The isolates hydrocarbon degradative activities were determined using turbidimetric analysis. The isolate with fastest growth was characterized based on morphology, biochemical test and subsequent molecular identification using 16S rRNA gene sequence analysis. Six bacteria strains; FS1, FS2, FS3, ES1, ES2 and ES3 that utilized 1% used engine oil as sole carbon and energy were isolated. Bacteria ES2 effectively utilized the used engine oil with the highest Optical density (OD) of 0.96 at 600 nm on the 10 th day. Result from the biochemical test, blasting and phylogenetic analysis showed that bacteria ES2 belongs to Lysinibacillus specie with 89% maximum identity to this specie. These bacteria can be used to effectively bio remediate hydrocarbon polluted environment.
烃污染是当今主要的环境问题之一,是石油化工相关活动的结果。石油烃因其复杂的结构、广泛的分布、毒性和持久性而成为人们关注的有机污染物。据报道,这些污染物在植物和动物组织中的积累会导致死亡或突变。为此,本研究对尼日利亚尼日州拉派市某汽车车间的烃污染土壤中分离的烃降解细菌进行了分子鉴定。以含1%废机油为碳源的矿物盐培养基(MSM)为研究对象进行了分离。采用比浊法测定分离物的烃降解活性。通过形态学、生化试验及16S rRNA基因序列分析对生长最快的分离物进行鉴定。6株细菌;分离出以1%废机油为唯一碳源和能量的FS1、FS2、FS3、ES1、ES2和ES3。细菌ES2有效利用了废机油,第10天在600 nm处光密度(OD)最高,为0.96。生化试验、爆破和系统发育分析结果表明,细菌ES2属于赖氨酸芽孢杆菌属,与赖氨酸芽孢杆菌属同源性最高达89%。利用这些细菌可以有效地对烃污染环境进行生物修复。
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引用次数: 2
Current Status of Multi Drug Resistance of Bacillus Species from Clinical Sources 临床来源芽孢杆菌多种耐药现状
Pub Date : 2019-10-05 DOI: 10.18782/2320-7051.7671
Amna Mahmood
Bacillus species are found anywhere in environment. Due to unnecessary use of antibiotics many bacteria are becoming resistant to antibiotics. Many bacterial strains become resistant due to the unnecessary use of antibiotics. When bacteria become resistant the antibiotic loses their ability for the elimination of bacteria from the body of infected person. 110 clinical samples were collected from different hospitals of Lahore. These samples were purified. After purification 20 Bacillus bacteria were isolated by using differential media MSA. Structures were examined under microscope after examination three bacillus species (B. subtillis, B. licheniformis and B. cereus) were found. For further confirmation biochemical tests were performed. Antimicrobial susceptibility test was performed at the end to check the bacterial susceptibility against antibiotics. Ten different antibiotics were used by disc diffusion method. Antibiotics were streptomycin, clindamycin, gentamicin, ampicillin, tetracycline, azithromycin, vancomycin, chloramphenicol, oxacillin and amoxicillin. 95% of the resistance was shown against oxacillin and 95% against ampicillin. 95% sensitivity was shown by streptomycin. 90% sensitivity was shown by tetracycline and gentamicin. 100% sensitivity was shown by three antibiotics against B. licheniformis which were streptomycin, tetracycline and gentamicin while no sensitivity was given by ampicillin, amoxicillin, oxacillin and clindamycin. 100% resistance was shown by oxacillin against B. licheniformis. No resistance was seen in the case of streptomycin, tetracycline and gentamicin. Maximum sensitivity was shown by streptomycin, tetracycline and gentamicin against B. subtilis. Minimum sensitivity was given by clindamycin. 100% resistance was shown by oxacillin and 89% resistance was given by amoxicillin. B. cereus is shown different percentages of resistance and sensitivity against ten different drugs. Maximum sensitivity was shown for streptomycin, tetracycline and gentamicin while maximum resistance was shown against ampicillin and oxacillin.
芽孢杆菌在环境中随处可见。由于不必要地使用抗生素,许多细菌对抗生素产生耐药性。许多菌株由于不必要地使用抗生素而产生耐药性。当细菌产生耐药性时,抗生素就失去了从感染者体内清除细菌的能力。从拉合尔不同医院收集了110份临床样本。这些样品被纯化。纯化后,采用差示培养基MSA分离得到20株芽孢杆菌。显微镜下观察结构,发现枯草芽孢杆菌、地衣芽孢杆菌和蜡样芽孢杆菌3种。为了进一步确认,进行了生化试验。最后进行药敏试验,检查细菌对抗生素的敏感性。采用圆盘扩散法对10种不同的抗生素进行检测。抗生素为链霉素、克林霉素、庆大霉素、氨苄西林、四环素、阿奇霉素、万古霉素、氯霉素、恶西林、阿莫西林。95%的耐药对象是奥西林,95%是氨苄西林。链霉素的敏感性为95%。四环素和庆大霉素的敏感性为90%。链霉素、四环素和庆大霉素对地衣双胞杆菌均有100%的敏感性,氨苄西林、阿莫西林、恶西林和克林霉素对地衣双胞杆菌均无敏感性。氧苄西林对地衣芽孢杆菌100%耐药。链霉素、四环素和庆大霉素均未见耐药。链霉素、四环素和庆大霉素对枯草芽孢杆菌的敏感性最高。克林霉素的敏感性最低。奥西林100%耐药,阿莫西林89%耐药。蜡样芽孢杆菌对10种不同药物的耐药和敏感性百分比不同。对链霉素、四环素和庆大霉素的敏感性最大,而对氨苄西林和奥西林的耐药性最大。
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引用次数: 0
Income Generation of Farmers via Medicinal Plants Cultivation: A Review 药用植物种植对农民创收的影响
Pub Date : 2019-10-05 DOI: 10.18782/2320-7051.7428
K. Singh
Medicinal and aromatic plants undertaken an important position in Ayurveda, Homeopathy and Unani medicine. These plants provide raw materials for pharmaceuticals, cosmetic, drug and other industries. Farmers collect more income via cultivation of the medicinal and aromatic plants than other commercial and traditional crops. Trading of medicinal plants worth globally stand over $ 5 billion and about INR 3 billion in India. National medicinal plant board, India provide subsidy of 30%, 50% and 75%, according to different plant species availability for production. About 95 medicinal plants species listed under National Ayush Mission (NAM) for cultivation. About 39 angiosperm plant species of herbs, shrubs and trees are found very common and some are wild in Uttar Pradesh and Uttarakhand state. Cultivation of medicinal and aromatic plants is the ideal alternative for some traditional uneconomic crops and help in medicinal plant protection.
药用和芳香植物在阿育吠陀、顺势疗法和乌纳尼医学中占有重要地位。这些植物为制药、化妆品、药品和其他工业提供原料。农民通过种植药用和芳香植物获得比其他商业和传统作物更多的收入。全球药用植物贸易价值超过50亿美元,印度约为30亿卢比。印度国家药用植物委员会根据不同的植物种类提供30%、50%和75%的补贴。大约95种药用植物被列入国家阿尤什任务(NAM)的种植范围。在北方邦和北阿坎德邦,大约有39种被子植物,草本植物,灌木和乔木非常常见,有些是野生的。药用和芳香植物的种植是一些传统不经济作物的理想替代品,有助于药用植物的保护。
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引用次数: 1
Effectiveness of Giving Health Education to Anxiety Levels for Primigravida Women Trimester III in Facing Labor as a Meeting of the Right to Inform 作为知情权会议的初产妇妊娠期面临分娩时焦虑程度健康教育的有效性
Pub Date : 2019-10-05 DOI: 10.18782/2320-7051.7613
Ni Made Karlina Sumiari Tangkas
Background: Most maternal deaths occur in labor and childbirth. This can be avoided through good antenatal services. The mother has the right to receive all information related to her condition. One of the competencies of midwives is providing education to mothers. IEC is an activity where there is a process of communication and education with the dissemination of information. This study aims to reduce the anxiety level of primigravida TW III mothers in facing labor. Method: This study is a pre-experimental study of one group pre-test post-test design with a prospective approach. Independent variables, namely IEC in pregnancy and the dependent variable, were decreased anxiety levels of third trimester primigravida mothers and fulfillment of the right to information. The population is all third trimester primigravida mothers who visit PMB or Buleleng Community Health Center work area 3. The total sample is 36 people, and the data collection technique uses an anxiety measure namely HARS sheet. Results: The results of the study showed that the statistical mean parametric t-test in the one-party test showed that t count = 9.88 was greater than t table = 1.684 so Ho was rejected and Ha was accepted which means IEC in pregnancy was effective in reducing the anxiety level of primigravida mothers trimester III and the right to obtain information is fulfilled. Conclusion: Giving IEC as one of the competencies of midwives that is effective in decreasing the anxiety level of pregnant women facing labor. The advice given to health workers at the Puskesmas must strive to improve the provision of counseling and especially IEC giving information that can reduce anxiety in primigravida pregnant women TW III.
背景:大多数产妇死亡发生在分娩和分娩。这可以通过良好的产前服务来避免。母亲有权获得与她的病情有关的一切信息。助产士的能力之一是为母亲提供教育。信息、教育和宣传是一项有传播信息的交流和教育过程的活动。本研究旨在降低初产妇临产时的焦虑水平。方法:本研究采用前瞻性的一组前测后测设计的实验前研究。独立变量,即怀孕期间的IEC和因变量是妊娠晚期初孕母亲焦虑水平的降低和知情权的实现。人口均为访问公共卫生局或布列冷社区卫生中心第3工作区的妊娠晚期初产妇。总样本为36人,数据收集技术采用焦虑量表即HARS表。结果:本研究结果显示,单方检验中的统计平均参数t检验显示t count = 9.88大于t table = 1.684,因此Ho被拒绝,Ha被接受,这意味着妊娠期IEC有效降低了初孕母亲III期的焦虑水平,实现了获得信息的权利。结论:将IEC作为助产士的能力之一,可有效降低临产孕妇的焦虑水平。向Puskesmas的保健工作者提供的建议必须努力改善咨询服务的提供,特别是提供信息教育,以减少初潮孕妇的焦虑。
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引用次数: 1
Acrylamide Mitigation of Fried Potato Chips by Using Amino Acids 利用氨基酸减少炸薯片中的丙烯酰胺
Pub Date : 2019-10-05 DOI: 10.18782/2320-7051.7590
T. Sivasakthi
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引用次数: 1
Panchagavya, An Organic Amendment for Inhibiting Damping-Off Causing Fusarium solani and Sclerotium rolfsii under In-vitro Conditions Panchagavya,一种有机添加剂,在体外条件下抑制引起番茄枯萎病和罗氏菌核病的阻尼
Pub Date : 2019-10-05 DOI: 10.18782/2320-7051.7693
M. S. Rathore
Damping-off is a serious fungal disease affecting seeds and seedlings in both nursery and field conditions. In this experiment, effectiveness of panchagavya in inhibiting the growth of two damping-off pathogens viz., Fusarium solani and Sclerotium rolfsii were evaluated under invitro conditions. Panchagavya has resulted in the inhibition up to 66.7% and 83.3% in Fusarium solani and up to 100% and 100% inhibition in Sclerotium rolfsii at 5% and 10% of panchagavya amended potato dextrose agar (PDA) plates, respectively.
湿落病是一种严重影响苗圃和田间种子和幼苗的真菌病害。本试验在体外条件下考察了panchagavya对两种消湿病原菌枯萎菌(Fusarium solani)和罗氏菌核菌(Sclerotium rolfsii)生长的抑制效果。Panchagavya在5%和10%的马铃薯葡萄糖琼脂(PDA)培养基上对茄枯病菌的抑制率分别为66.7%和83.3%,对罗氏菌核菌的抑制率分别为100%和100%。
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引用次数: 2
Long Term Effect of Integrated Nutrient Management on Soil Micronutrient Status in Finger Millet Mono-Cropping System 综合养分管理对小谷子单作土壤微量元素状况的长期影响
Pub Date : 2019-10-05 DOI: 10.18782/2320-7051.7686
DV Prashanth, R. Krishnamurthy, DV Naveen
Soil macronutrients were studied on Alfisols with a 40-years old experiment at All India Co-ordinated Research Project for Dry land Agriculture (AICRPDA), Gandhi Krishi Vignana Kendra, University of Agricultural Sciences, Bengaluru, during kharif 2018. Results indicated that continuous application of chemical fertilizers and manures increased the micronutrient availability in soil and uptake by finger millet. Long term integrated nutrient management showed significant higher values of available N of 345.35 kg ha, P2O5 of 66.58 kg ha, K2O content of 136 kg ha, for soils in a treatment T5 FYM @ 10 t ha + 100% RDF. Lower values were recorded for the control T1 where no fertilizer was added. Similar trend of results were reported Nutrient content and nutrient uptake by finger millet for Application of FYM @ 10 t ha + 100% RDF over a period of 40 years significantly increased the macronutrient content in soil and uptake by finger millet as against 100% NPK treatment and control.
2018年秋季,班加罗尔农业科学大学甘地·克里希·维格纳·肯德拉(Gandhi Krishi Vignana Kendra)在全印度旱地农业协调研究项目(AICRPDA)进行了一项已有40年历史的试验,研究了Alfisols上的土壤宏量营养素。结果表明,连续施用化肥和粪肥可提高土壤微量元素有效性和谷子对土壤微量元素的吸收。长期综合养分管理表明,在T5 FYM @ 10 t ha + 100% RDF处理下,土壤有效氮值显著高于345.35 kg ha, P2O5含量为66.58 kg ha, K2O含量为136 kg ha。在不施肥的对照T1中,记录的数值较低。与100%氮磷钾处理和对照相比,施用FYM @ 10 t ha + 100% RDF的40年土壤中微量元素含量和谷子的养分吸收显著增加。
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引用次数: 3
Effect of IBA on Rooting of Hardwood Cuttings of Various Peach Genotypes IBA对不同基因型桃硬木插条生根的影响
Pub Date : 2019-10-05 DOI: 10.18782/2320-7051.7575
G. Singh
An investigation was conducted in the experimental plot of nursery of Department of Horticulture, Khalsa College, Amritsar during 2018-2019 consisting of hardwood cuttings of 20 cm length and 1.2cm diameter with 5-6 buds prepared from the dormant twigs of the 7-8 years old of Pratap, Florda Prince and Shan-i-Punjab peach cultivars in the second fortnight of November. The basal portion of the cuttings was dipped in different doses of IBA (1000, 1500, 2000, 2500 and 3000 ppm) and control were evaluated. On the basis of data recorded on rooting parameters after 45 days of planting, it was concluded that rooting parameters like root length, root thickness, length of main root, number of roots per cutting and root weight were found maximum in Shan-i-Punjab when treated with IBA 3000ppm. It was concluded that IBA at 3000 ppm was found to be the best treatment for propagation of peach through hardwood cuttings.
2018-2019年,在阿姆利则哈尔萨学院园艺系苗圃试验田进行了调查,在11月的第二周,从7-8岁的Pratap、florida Prince和Shan-i-Punjab品种的休眠枝上制备了长20厘米、直径1.2厘米的硬木插条,芽5-6个。以不同剂量的IBA(1000、1500、2000、2500和3000 ppm)浸泡在扦插的基部,评价其防治效果。结果表明,当IBA浓度为3000ppm时,单根长、根粗、主根长、单根数、根重等根系参数最大。结果表明,3000 ppm的IBA处理是桃木扦插繁殖的最佳处理。
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引用次数: 0
Assessment of Chank Fishing as Livelihood in Therespuram, Tuticorin 图蒂戈林Therespuram地区渔业生计评估
Pub Date : 2019-10-05 DOI: 10.18782/2320-7051.7648
V. Gomathy
Chank fishing is one of the oldest professions carried out through traditional diving methods in coastal waters of Tamil Nadu. From generations, fishers have progressed from stone and rope to scuba equipmenst and other locally designed contraptions for diving into waters. Current study had been carried out to assess the role of chank fishery in fisher’s livelihood of Therespuram fishing village, Tuticorin. Primary data was collected from purposively selected forty fishers by using structured interview schedule. Results of this study reveals that 45 % of middle aged (3545 years) fisher’s were found to be engaged in chank diving. Fishers below 35 years of age were found to be educated but could earn less than their counter parts of middle aged ones. Fisher’s physical fitness and chank collection skills are essential traits for chank diving. Chank fishing was reported as the primary source of income for these fisher’s and their average net income was estimated as ₹ 12 lakhs/annum/boat. They spend majorily (65%) on food items followed by individual health care and children education. Due to prolonged underwater exposure, health problems followed by debt were reported as major constraints by the fishers. By performing a skilled job like chank fishing, fisher’s had to face several occupational hazards in coastal waters. Thus, appropriate onshore medical facility in case of emergency, adequate training towards scientific diving, uses of improved diving equipment and alternative employment during off season are utmost important to enhance fisher’s livelihood in Therespuram village.
在泰米尔纳德邦沿海水域,Chank捕鱼是通过传统潜水方法进行的最古老的职业之一。几代人以来,渔民们已经从石头和绳子发展到水肺设备和其他当地设计的潜水装置。目前进行的研究是评估长链渔业对图蒂戈林Therespuram渔村渔民生计的作用。采用结构化访谈法,有目的地选取40名渔民进行初步资料收集。本研究结果显示,在中年(3545岁)渔民中,有45%从事潜水。35岁以下的渔民受教育程度较高,但收入低于中年渔民。费舍尔的身体素质和收集鱼钩的技巧是鱼钩潜水的基本特征。据报道,捕鱼是这些渔民的主要收入来源,他们的平均净收入估计为每年每艘船120万卢比。他们的主要支出(65%)是食品,其次是个人保健和儿童教育。据报告,由于长期暴露在水下,健康问题和债务问题是渔民面临的主要制约因素。在沿海水域,渔民从事像长杆捕鱼这样的技巧性工作时,不得不面对几种职业危害。因此,在紧急情况下提供适当的岸上医疗设施、进行充分的科学潜水培训、使用改进的潜水设备以及在淡季提供替代就业,对于改善Therespuram村渔民的生计至关重要。
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引用次数: 0
In vitro Analysis of Rhizobacteria against Macrophomina phaseolina (Tassi) Goid. Causing Charcoal Rot of Sorghum 根瘤菌体外抗菜绿巨噬菌分析。引起高粱炭腐病的研究
Pub Date : 2019-10-05 DOI: 10.18782/2320-7051.7692
Vinayaka A. Bannur
A total of seven rhizobacterial isolates (AUDT 240, AUDT 248, AUDT 502, AUDT 801, AUDT 807, AUDT 811and AUDP 139) and one reference isolate of Trichoderma harzianum were examined for antagonistic activities against five different isolates of Macrophomina phaseolina viz., Belavadi, Dharwad, Kalaburagi, Savanur and Vijayapura. The isolate AUDP 139 (Pseudomonas fluorescens) showed maximum mean per cent inhibition (56.50 %) followed by AUDT 240 (47.24 %) and AUDT 801(46.33 %). Results indicated that the isolates AUDP 139, AUDT 240 and AUDT 801 could be a novel biocontrol agents for the management of charcoal rot of sorghum.
研究了7株根瘤菌(AUDT 240、AUDT 248、AUDT 502、AUDT 801、AUDT 807、AUDT 811和AUDP 139)和1株哈兹木霉(Trichoderma harzianum)对5株不同菌株(Belavadi、Dharwad、Kalaburagi、Savanur和Vijayapura)的拮抗活性。分离物AUDP 139(荧光假单胞菌)的平均抑制率最高(56.50%),其次是AUDT 240(47.24%)和AUDT 801(46.33%)。结果表明,分离菌株audp139、audt240和audt801可作为防治高粱炭腐病的新型生物防治剂。
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引用次数: 0
期刊
International Journal of Pure & Applied Bioscience
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