For years, medicinal plants have been a primary source of rectifiers for healthcare. Information about how plants work is either passeddown from elders, learned through experience or via trials, but it isn’t always proven. A similarly extensive repository of ethnomedicalknowledge is North Haryana. The present study demonstrate the importance of plants from the Hisar district in the state of Haryana forethnomedicine. Field checks and in-depth interviews conducted in the fields between 2022 and 2023 were used to gather the data. Thecurrent study contains thorough ethnomedical information, including common and botanical names, family names, pharmaceuticaldelivery methods, lozenge dosage forms, and ailments addressed. It was determined that this region still has a wealth of practicalethnomedical expertise and may contribute to further herbal medicine development programs.
{"title":"Study of Ethnomedicinal Plants Found in Different Villages of Hisar, Haryana in Winter Season","authors":"Gajanand Modi, Babita Babita","doi":"10.18811/ijpen.v9i03.12","DOIUrl":"https://doi.org/10.18811/ijpen.v9i03.12","url":null,"abstract":"For years, medicinal plants have been a primary source of rectifiers for healthcare. Information about how plants work is either passeddown from elders, learned through experience or via trials, but it isn’t always proven. A similarly extensive repository of ethnomedicalknowledge is North Haryana. The present study demonstrate the importance of plants from the Hisar district in the state of Haryana forethnomedicine. Field checks and in-depth interviews conducted in the fields between 2022 and 2023 were used to gather the data. Thecurrent study contains thorough ethnomedical information, including common and botanical names, family names, pharmaceuticaldelivery methods, lozenge dosage forms, and ailments addressed. It was determined that this region still has a wealth of practicalethnomedical expertise and may contribute to further herbal medicine development programs.","PeriodicalId":14298,"journal":{"name":"INTERNATIONAL JOURNAL OF PLANT AND ENVIRONMENT","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139335150","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The current study was carried out to delineate the effect of cement industry effluent on the two screened varieties of R. communis(Castor) viz. MSC-55 and Western-6. A number of analyses such as growth parameters, photosynthetic pigment, metal accumulationand antioxidant enzyme were performed on both the varieties. With respect to T0 (100% tap water), an increase of 86.84, 90.97 and80.15% was noticed in the fresh weight (FW) of roots of MSC-55 variety at 30, 60 and 90 DAS, respectively, when irrigated with T2 (50%effluent+50% tap water). The results obtained significantly differed between T0 and T2 and T0 and T4 (at p ≤ 0.05). MSC-55 variety wasobserved to contain an increased amount of total chlorophyll and carotenoids up to T2 concentration which declined at higher effluentconcentration i.e., T4 (100% effluent). Increased concentration of Cement Industry Effluent (CIE) enhanced the generation of someantioxidant compounds like Guaiacol peroxidase (GPX) and Ascorbate peroxidase (APX) in both the varieties up to certain treatmentconcentrations viz. MSC-55 and Western-6. As the effluent concentration increased in both the varieties, the amount of malondialdehyde(MDA) in the leaves and roots also rose. With increasing effluent concentrations, Catalase (CAT) and Superoxide dismutase (SOD)also increased. MSC-55 variety of R. communis was observed to have a strong antioxidant defence system against CIE and could berecommended for cultivation during metal-contaminated industrial effluent condition.
{"title":"Growth, Metal Accumulation Potential and Antioxidant Enzyme Responses of Ricinus communis L. Genotypes Exposed to Cement Industry Effluent","authors":"K. Bauddh, Ankit Ankit, Amit Kumar, Mohammad Amir","doi":"10.18811/ijpen.v9i03.10","DOIUrl":"https://doi.org/10.18811/ijpen.v9i03.10","url":null,"abstract":"The current study was carried out to delineate the effect of cement industry effluent on the two screened varieties of R. communis(Castor) viz. MSC-55 and Western-6. A number of analyses such as growth parameters, photosynthetic pigment, metal accumulationand antioxidant enzyme were performed on both the varieties. With respect to T0 (100% tap water), an increase of 86.84, 90.97 and80.15% was noticed in the fresh weight (FW) of roots of MSC-55 variety at 30, 60 and 90 DAS, respectively, when irrigated with T2 (50%effluent+50% tap water). The results obtained significantly differed between T0 and T2 and T0 and T4 (at p ≤ 0.05). MSC-55 variety wasobserved to contain an increased amount of total chlorophyll and carotenoids up to T2 concentration which declined at higher effluentconcentration i.e., T4 (100% effluent). Increased concentration of Cement Industry Effluent (CIE) enhanced the generation of someantioxidant compounds like Guaiacol peroxidase (GPX) and Ascorbate peroxidase (APX) in both the varieties up to certain treatmentconcentrations viz. MSC-55 and Western-6. As the effluent concentration increased in both the varieties, the amount of malondialdehyde(MDA) in the leaves and roots also rose. With increasing effluent concentrations, Catalase (CAT) and Superoxide dismutase (SOD)also increased. MSC-55 variety of R. communis was observed to have a strong antioxidant defence system against CIE and could berecommended for cultivation during metal-contaminated industrial effluent condition.","PeriodicalId":14298,"journal":{"name":"INTERNATIONAL JOURNAL OF PLANT AND ENVIRONMENT","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139334935","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Soil health and nutrient availability are important for sustaining crop productivity and increasing yield. Farmers have practiced fortifyingsoil using various supplements (organic and inorganic, natural and synthetic) since the advent of modern agriculture. However, addinginorganic fertilizers, weedicides and pesticides has adversely affected our agroecosystems. Harmful chemicals from synthetic fertilizersget accumulated in soil and plant parts, causing serious health problems. The synthetic agri-products have detrimental effects onsoil fertility and soil microbiota, making the soil less fertile and unsuitable for cultivation. In the last decade, a paradigm shift in agripracticeshas occurred and organic farming has become a popular sustainable option to improve soil health and achieve sustainabledevelopment goals.The potential of various organic supplements has been studied in the present investigation on the growth parameters of an importantvegetable crop okra (Abelmoschus esculentus) valued for its multipurpose uses in various parts of the country. Pure line seeds of okravariety Arka Anamika were surface sterilized and used for the study. Plants were raised in plastic pots (10 inches) filled with soil treatedwith leaf compost, foliar spray (Gibberellic acid, 0.001%) and pine bark mulch. Garden soil without organic supplements served asa control. The experiment was carried out in a completely randomized design with three replications. An increase in the vegetativegrowth parameters and in reproductive parameters in all the treatments as compared to control was observed in the plants. Though alltreatments showed an increase in growth parameters, a significant increase was observed with mulch, suggesting that the mulch derivedfrom organic waste and residues have immense potential in improving crop health by making them more robust, and enhancing theyield. Investigating the amelioration of soil physico-chemical properties with these organic supplements and understanding their role inbetter crop performance is beneficial for the farmers. The present study also provides simple, sustainable, and eco-friendly biofertilizerpotential of self-prepared pine bark mulch for vegetable crops.
土壤健康和养分供应对于维持作物生产力和提高产量非常重要。自现代农业出现以来，农民一直在使用各种补充剂（有机和无机、天然和合成）强化土壤。然而，添加无机肥料、除草剂和杀虫剂对我们的农业生态系统产生了不利影响。合成肥料中的有害化学物质在土壤和植物体内积累，造成严重的健康问题。合成农用产品对土壤肥力和土壤微生物群产生有害影响，使土壤肥力降低，不适合耕种。在过去的十年中，农业实践的模式发生了转变，有机耕作已成为改善土壤健康和实现可持续发展目标的一种流行的可持续选择。本调查研究了各种有机补充剂对一种重要的蔬菜作物秋葵（Abelmoschus esculentus）生长参数的影响。黄秋葵品种 Arka Anamika 的纯系种子经表面消毒后用于研究。植物在塑料盆（10 英寸）中培育，盆中的土壤经过叶面堆肥、叶面喷洒（赤霉素，0.001%）和松树皮覆盖处理。未添加有机添加剂的花园土壤作为对照。实验采用完全随机设计，三次重复。与对照相比，所有处理的植株无性生长参数和生殖参数都有所增加。虽然所有处理的生长参数都有所提高，但地膜覆盖的生长参数显著提高，这表明有机废物和残留物制成的地膜在改善作物健康方面具有巨大潜力，能使作物更加健壮，提高产量。研究这些有机补充物对土壤理化性质的改善作用，并了解它们在改善作物表现方面的作用，对农民来说是有益的。本研究还提供了自制松树皮覆盖物用于蔬菜作物的简单、可持续和生态友好型生物肥料的潜力。
{"title":"Evaluation of Morphological Growth Response of Abelmoschus esculentus (Okra) to Simple Organic Supplements","authors":"Inderdeep Kaur, Preeti Kaur, Jaskaran Singh, Monika Koul","doi":"10.18811/ijpen.v9i03.11","DOIUrl":"https://doi.org/10.18811/ijpen.v9i03.11","url":null,"abstract":"Soil health and nutrient availability are important for sustaining crop productivity and increasing yield. Farmers have practiced fortifyingsoil using various supplements (organic and inorganic, natural and synthetic) since the advent of modern agriculture. However, addinginorganic fertilizers, weedicides and pesticides has adversely affected our agroecosystems. Harmful chemicals from synthetic fertilizersget accumulated in soil and plant parts, causing serious health problems. The synthetic agri-products have detrimental effects onsoil fertility and soil microbiota, making the soil less fertile and unsuitable for cultivation. In the last decade, a paradigm shift in agripracticeshas occurred and organic farming has become a popular sustainable option to improve soil health and achieve sustainabledevelopment goals.The potential of various organic supplements has been studied in the present investigation on the growth parameters of an importantvegetable crop okra (Abelmoschus esculentus) valued for its multipurpose uses in various parts of the country. Pure line seeds of okravariety Arka Anamika were surface sterilized and used for the study. Plants were raised in plastic pots (10 inches) filled with soil treatedwith leaf compost, foliar spray (Gibberellic acid, 0.001%) and pine bark mulch. Garden soil without organic supplements served asa control. The experiment was carried out in a completely randomized design with three replications. An increase in the vegetativegrowth parameters and in reproductive parameters in all the treatments as compared to control was observed in the plants. Though alltreatments showed an increase in growth parameters, a significant increase was observed with mulch, suggesting that the mulch derivedfrom organic waste and residues have immense potential in improving crop health by making them more robust, and enhancing theyield. Investigating the amelioration of soil physico-chemical properties with these organic supplements and understanding their role inbetter crop performance is beneficial for the farmers. The present study also provides simple, sustainable, and eco-friendly biofertilizerpotential of self-prepared pine bark mulch for vegetable crops.","PeriodicalId":14298,"journal":{"name":"INTERNATIONAL JOURNAL OF PLANT AND ENVIRONMENT","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139334953","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Samridhi Malviya, Girish C. Pathak, Shyam N. Pandey, Shyam N. Pandey
An experiment was conducted to study the effect of various zinc amendment levels as well as interactions with iron doses in soil on the growth of plant (length and dry weight), some biochemical parameters such as plant pigments, protein contents, and activity of some enzymes such as catalase and peroxidase in Phaseolus vulgaris (French bean). The amendment of zinc and iron was made as Native soil, 5mg ZnSO4, 25 mg ZnSO4, 50 mg ZnSO4 and 100 mg kg-1 ZnSO4 in soil and their interactive doses with FeSO4 was made as Native soil, 25 mg FeSO4, 25 mg FeSO4 + 25 mg ZnSO4, 50 mg FeSO4 + 5 mg ZnSO4 and 25 mg FeSO4 + 50 mg kg-1 ZnSO4 in soil. The experiment was conducted in triplicates. The maximum value of the dry weight, pigments, and protein content was found at the application of 50 mg kg-1 ZnSO4 + 25 mg kg-1 FeSO4 in soil. The increase in dry weight, total chlorophyll, and protein contents by 48, 44.4, and 37.3 were observed maximum in French bean at the application of 50 mg kg-1 ZnSO4 soil and 25 mg kg-1 FeSO4 in the soil. Maximum tissue accumulation of Fe was found at the high dose of Fe with low Zn-dose, the tissue accumulation showed antagonistic effects of zinc and iron.
{"title":"Responses of Phaseolus vulgaris towards Zinc and Iron Management in Soil with respect to Growth, Pigments and Protein Contents","authors":"Samridhi Malviya, Girish C. Pathak, Shyam N. Pandey, Shyam N. Pandey","doi":"10.18811/ijpen.v9i02.12","DOIUrl":"https://doi.org/10.18811/ijpen.v9i02.12","url":null,"abstract":"An experiment was conducted to study the effect of various zinc amendment levels as well as interactions with iron doses in soil on the growth of plant (length and dry weight), some biochemical parameters such as plant pigments, protein contents, and activity of some enzymes such as catalase and peroxidase in Phaseolus vulgaris (French bean). The amendment of zinc and iron was made as Native soil, 5mg ZnSO4, 25 mg ZnSO4, 50 mg ZnSO4 and 100 mg kg-1 ZnSO4 in soil and their interactive doses with FeSO4 was made as Native soil, 25 mg FeSO4, 25 mg FeSO4 + 25 mg ZnSO4, 50 mg FeSO4 + 5 mg ZnSO4 and 25 mg FeSO4 + 50 mg kg-1 ZnSO4 in soil. The experiment was conducted in triplicates. The maximum value of the dry weight, pigments, and protein content was found at the application of 50 mg kg-1 ZnSO4 + 25 mg kg-1 FeSO4 in soil. The increase in dry weight, total chlorophyll, and protein contents by 48, 44.4, and 37.3 were observed maximum in French bean at the application of 50 mg kg-1 ZnSO4 soil and 25 mg kg-1 FeSO4 in the soil. Maximum tissue accumulation of Fe was found at the high dose of Fe with low Zn-dose, the tissue accumulation showed antagonistic effects of zinc and iron.","PeriodicalId":14298,"journal":{"name":"INTERNATIONAL JOURNAL OF PLANT AND ENVIRONMENT","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-08-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135620901","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Cissus quadrangularis Linn. is a succulent perennial plant of family Vitaceae also called as Asthisandhaanak or Hadjor in Hindi, has been traditionally described in Ayurveda and Siddha literature as general tonic and as a powerful analgesic, used as an anti-cancer, antidiabetic, antibacterial, hepatoprotective and neuroprotective etc. It is a good source of biologically active secondary metabolites withvarious pharmacological activities implicated in a wide range of human diseases. Cancer is a major issue or concern in public health systems, especially in developing countries like India. Matrix metalloproteinases (MMP), Tyrosine kinase (TK) and Vascular endothelial growth factor (VEGF) are emerging as an important cancer target therapeutic proteins. Molecular docking studies provide a better insight into the biological activity of secondary metabolites like Resveratrol (3,4′,5-trans-trihydroxystilbene) and Piceatannol (3,3′,4,5′-transtrihydroxystilbene) from C. quadrangularis L., its possible mechanisms of action, binding modes and predicting it as a possible anti-cancer drug with and lesser or no side effects
{"title":"In-silico Molecular Interaction Studies of Biologically Active Secondary Metabolites of Cissus quadrangularis L. as a Potential Anti-cancer Drug","authors":"Abhinav Chauhan, Arvind Kumar, Tanuja .","doi":"10.18811/ijpen.v9i02.10","DOIUrl":"https://doi.org/10.18811/ijpen.v9i02.10","url":null,"abstract":"Cissus quadrangularis Linn. is a succulent perennial plant of family Vitaceae also called as Asthisandhaanak or Hadjor in Hindi, has been traditionally described in Ayurveda and Siddha literature as general tonic and as a powerful analgesic, used as an anti-cancer, antidiabetic, antibacterial, hepatoprotective and neuroprotective etc. It is a good source of biologically active secondary metabolites withvarious pharmacological activities implicated in a wide range of human diseases. Cancer is a major issue or concern in public health systems, especially in developing countries like India. Matrix metalloproteinases (MMP), Tyrosine kinase (TK) and Vascular endothelial growth factor (VEGF) are emerging as an important cancer target therapeutic proteins. Molecular docking studies provide a better insight into the biological activity of secondary metabolites like Resveratrol (3,4′,5-trans-trihydroxystilbene) and Piceatannol (3,3′,4,5′-transtrihydroxystilbene) from C. quadrangularis L., its possible mechanisms of action, binding modes and predicting it as a possible anti-cancer drug with and lesser or no side effects","PeriodicalId":14298,"journal":{"name":"INTERNATIONAL JOURNAL OF PLANT AND ENVIRONMENT","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-08-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135620898","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The Dumas method (CHNS analyzer) is replacing the traditional Kjeldahl method as the method of choice for N analysis due to advancements in dry combustion nitrogen (N) analyzer technology and the high cost of disposing of hazardous laboratory waste chemicals. As a result, a comparison of the Dumas method (CHNS analyzer) with the Kjeldahl method is critical. Typically, such comparisons were conducted on a small number of distinct samples. The goal of this study was to compare the performance of instruments that use the automated Dumas method (CHNS analyzer) and the Kjeldahl method for N analysis of agricultural materials in a high-throughput laboratory setting. Using both instruments, N concentrations in manure, sewage sludge, plant tissue, plant seeds, and feedstuff fibres were determined. We collected samples for the analysis of organic matter from the horticulture area at the Banaras Hindu University campus in Varanasi, and total Nitrogen (N) was estimated using the Kjeldahl (wet oxidation) and Perkin-Elmer 2.400 Series II-CHN Mode techniques (dry oxidation or combustion). The positive correlation (r = 0.94**) of the Kjeldahl method with N- determination from a CHNS analyzer demonstrates that the method is practical for routinely determining the total nitrogen content of soils
{"title":"Comparison of Total Nitrogen estimation by Kjeldahl Method and CHNS Analyzer in Dry Tropical Grassland","authors":"Vijay P. Gautam, Swati Mishra, Haseen Ahmed","doi":"10.18811/ijpen.v9i02.13","DOIUrl":"https://doi.org/10.18811/ijpen.v9i02.13","url":null,"abstract":"The Dumas method (CHNS analyzer) is replacing the traditional Kjeldahl method as the method of choice for N analysis due to advancements in dry combustion nitrogen (N) analyzer technology and the high cost of disposing of hazardous laboratory waste chemicals. As a result, a comparison of the Dumas method (CHNS analyzer) with the Kjeldahl method is critical. Typically, such comparisons were conducted on a small number of distinct samples. The goal of this study was to compare the performance of instruments that use the automated Dumas method (CHNS analyzer) and the Kjeldahl method for N analysis of agricultural materials in a high-throughput laboratory setting. Using both instruments, N concentrations in manure, sewage sludge, plant tissue, plant seeds, and feedstuff fibres were determined. We collected samples for the analysis of organic matter from the horticulture area at the Banaras Hindu University campus in Varanasi, and total Nitrogen (N) was estimated using the Kjeldahl (wet oxidation) and Perkin-Elmer 2.400 Series II-CHN Mode techniques (dry oxidation or combustion). The positive correlation (r = 0.94**) of the Kjeldahl method with N- determination from a CHNS analyzer demonstrates that the method is practical for routinely determining the total nitrogen content of soils","PeriodicalId":14298,"journal":{"name":"INTERNATIONAL JOURNAL OF PLANT AND ENVIRONMENT","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-08-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135620720","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The family Apocynaceae comprises three species of the substantially important Alstonia plant, viz., A. scholaris, A. venenata, and A. macrophylla. The investigation of proteins contained within seeds, that has the potential to provide both precise details and a structural basis for characterizing diversity. The utilization of an electrophoretic technique for protein analysis has been observed in recent scholarly investigations. This implies that certain protein bands exhibit variability, with their presence or absence being detected across different seed arrangement levels in the gel. Furthermore, this implies that the protein bands have been segregated into distinct categories. A study was conducted to analyze the seed storage protein profiles of three distinct Alstonia species through the application of sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE). The resolution of the seed storage protein of the Alstonia species using 15% SDS was found to alter the banding pattern of the polyacrylamide gel. The SDS-PAGE analysis revealed differential up regulation of proteins across distinct bands. Throughout the examination, it was determined that the three aforementioned species exhibited acommon band, in addition to a protein with a molecular weight of 34 kDa. The number of protein bands attached to A. venenata was the highest (ten bands), while the number of protein bands that adhered to A. scholaris was the lowest (five bands). Further, powdered leaves of A. scholaris, A. venenata, and A. macrophylla were investigated for antibacterial and the results, A. macrophylla leaf powder is most effective against Staphylococcus aureus, followed by A. venenata and A. scholaris at 1 μg/ mL. In addition, the findings support the inference that the A. scholaris leaf powder effectively inhibited the growth of Aspergillus niger. A. venenata and A. macrophylla, both at 1μg/mL, are, nonetheless, also effective against A. niger. A. macrophylla has the largest zone of inhibition (11.5 mm) against A. niger.
夹竹桃科包括三个非常重要的Alstonia植物，即A. scholaris, A. venenata和A. macrophylla。对种子中所含蛋白质的研究，有可能为描述多样性提供精确的细节和结构基础。在最近的学术研究中，已经观察到蛋白质分析的电泳技术的使用。这意味着某些蛋白质带表现出可变性，它们的存在或不存在在凝胶中不同的种子排列水平上被检测到。此外，这意味着蛋白质条带被分离成不同的类别。采用十二烷基硫酸钠聚丙烯酰胺凝胶电泳(SDS-PAGE)技术，对3种不同的阿尔斯通属植物的种子贮藏蛋白谱进行了分析。研究发现，15% SDS对Alstonia种子贮藏蛋白的溶解可以改变聚丙烯酰胺凝胶的条带模式。SDS-PAGE分析显示不同波段的蛋白上调差异。在整个检查过程中，确定上述三个物种除了具有分子量为34 kDa的蛋白质外，还具有一个共同的条带。黄花蒿附着蛋白带数最多(10条)，黄花蒿附着蛋白带数最少(5条)。进一步研究了金针叶粉、金针叶粉和大叶粉对金黄色葡萄球菌的抑菌效果，结果表明，在1 μg/ mL浓度下，金针叶粉对金黄色葡萄球菌的抑菌效果最好，其次是金针叶粉和金针叶粉。此外，研究结果支持金针叶粉对黑曲霉生长有抑制作用的推断。尽管如此，1μg/mL的venenata和macrophylla对黑曲霉也有效。大叶蚜对黑蚜的抑制区最大(11.5 mm)。
{"title":"Variation in Antimicrobial Activity and Seed Storage Proteins in Three Species of the Medicinal Plant Alstonia","authors":"Jyoti Kumari, Tanuja .","doi":"10.18811/ijpen.v9i02.09","DOIUrl":"https://doi.org/10.18811/ijpen.v9i02.09","url":null,"abstract":"The family Apocynaceae comprises three species of the substantially important Alstonia plant, viz., A. scholaris, A. venenata, and A. macrophylla. The investigation of proteins contained within seeds, that has the potential to provide both precise details and a structural basis for characterizing diversity. The utilization of an electrophoretic technique for protein analysis has been observed in recent scholarly investigations. This implies that certain protein bands exhibit variability, with their presence or absence being detected across different seed arrangement levels in the gel. Furthermore, this implies that the protein bands have been segregated into distinct categories. A study was conducted to analyze the seed storage protein profiles of three distinct Alstonia species through the application of sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE). The resolution of the seed storage protein of the Alstonia species using 15% SDS was found to alter the banding pattern of the polyacrylamide gel. The SDS-PAGE analysis revealed differential up regulation of proteins across distinct bands. Throughout the examination, it was determined that the three aforementioned species exhibited acommon band, in addition to a protein with a molecular weight of 34 kDa. The number of protein bands attached to A. venenata was the highest (ten bands), while the number of protein bands that adhered to A. scholaris was the lowest (five bands). Further, powdered leaves of A. scholaris, A. venenata, and A. macrophylla were investigated for antibacterial and the results, A. macrophylla leaf powder is most effective against Staphylococcus aureus, followed by A. venenata and A. scholaris at 1 μg/ mL. In addition, the findings support the inference that the A. scholaris leaf powder effectively inhibited the growth of Aspergillus niger. A. venenata and A. macrophylla, both at 1μg/mL, are, nonetheless, also effective against A. niger. A. macrophylla has the largest zone of inhibition (11.5 mm) against A. niger.","PeriodicalId":14298,"journal":{"name":"INTERNATIONAL JOURNAL OF PLANT AND ENVIRONMENT","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-08-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135620721","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
In today’s global context, the escalation of air pollution stands out as an immensely critical environmental challenge that has attained a worldwide magnitude. This pressing issue not only impacts every living organism on our planet but is also intricately linked to the phenomenon of climate change. The significant increase in vehicular traffic, rapid urbanization, and infrastructure development have indirectly contributed to a higher concentration of harmful gaseous and particulate pollutants in the atmosphere, posing serious risks to human health. Extensive research has thoroughly documented the adverse effects of these air pollutants, with mortality and morbidity rates varying depending on the type of pollutant and the duration of exposure. However, amidst this crisis, green plants emerge as a cost-effective and promising solution to combat environmental pollution, presenting several additional benefits. Specifically, pollutiontolerant plant species are crucial in reducing ambient air pollution and the urban heat island effect. To assess a plant’s tolerance towards air pollution, experts use the air pollution tolerance index (APTI), which calculates crucial factors such as ascorbic acid, total chlorophyll, pH, and relative water content in the plant. This determination provides a reliable method for categorizing plants into either tolerant or sensitive types in the face of air pollution. Moreover, the morphological characteristics of leaves, such as stomata distribution and density, cuticle thickness, and trichome density, play an essential role in adsorbing and absorbing particulate matter from the air. These inherent qualities further enhance plants’ potential to combat air pollution in a sustainable manner, making them valuable assets for the future. In light of this, the present review highlights the impressive capacity of plants to remediate air pollutants and explores the various strategies employed in this crucial endeavor. By leveraging the remarkable capabilities of green plants, we have the opportunity to address the air pollution crisis and embrace a sustainable path for the times to come. These remarkable organisms could be the sustainable saviors we need to protect our environment and secure a healthier future for all.
{"title":"Green Plants as a Sustainable Solution to Air Pollution","authors":"Nitin Joshi, Charu Khosla Gupta, Yash Mangla, Arijit Chowdhuri","doi":"10.18811/ijpen.v9i02.02","DOIUrl":"https://doi.org/10.18811/ijpen.v9i02.02","url":null,"abstract":"In today’s global context, the escalation of air pollution stands out as an immensely critical environmental challenge that has attained a worldwide magnitude. This pressing issue not only impacts every living organism on our planet but is also intricately linked to the phenomenon of climate change. The significant increase in vehicular traffic, rapid urbanization, and infrastructure development have indirectly contributed to a higher concentration of harmful gaseous and particulate pollutants in the atmosphere, posing serious risks to human health. Extensive research has thoroughly documented the adverse effects of these air pollutants, with mortality and morbidity rates varying depending on the type of pollutant and the duration of exposure. However, amidst this crisis, green plants emerge as a cost-effective and promising solution to combat environmental pollution, presenting several additional benefits. Specifically, pollutiontolerant plant species are crucial in reducing ambient air pollution and the urban heat island effect. To assess a plant’s tolerance towards air pollution, experts use the air pollution tolerance index (APTI), which calculates crucial factors such as ascorbic acid, total chlorophyll, pH, and relative water content in the plant. This determination provides a reliable method for categorizing plants into either tolerant or sensitive types in the face of air pollution. Moreover, the morphological characteristics of leaves, such as stomata distribution and density, cuticle thickness, and trichome density, play an essential role in adsorbing and absorbing particulate matter from the air. These inherent qualities further enhance plants’ potential to combat air pollution in a sustainable manner, making them valuable assets for the future. In light of this, the present review highlights the impressive capacity of plants to remediate air pollutants and explores the various strategies employed in this crucial endeavor. By leveraging the remarkable capabilities of green plants, we have the opportunity to address the air pollution crisis and embrace a sustainable path for the times to come. These remarkable organisms could be the sustainable saviors we need to protect our environment and secure a healthier future for all.","PeriodicalId":14298,"journal":{"name":"INTERNATIONAL JOURNAL OF PLANT AND ENVIRONMENT","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-08-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135620722","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Diabetes mellitus is a disorder whose frequency is speedily increasing everywhere on the planet. According to the World HealthOrganization (WHO), a United Nations Agency, polygenic diseases are going to be the seventh leading reason for death in 2030. Several healthful plants like Citrullus lanatus, Trigonella foenum-graecum, and Murraya koenigii are used for the treatment of diabetes disorders in the Indian medicine system and different ancient systems of the world. These three plant leaves were collected from Vadodara and Anand, Gujarat. Thin layer chromatography profiling of all three plant leaves confirmed the presence of various common classes of phytochemicals such as rutin, gallic acid, tannic acid, and quercetin in both cities. Thin layer chromatographic analysis of a particular leaf revealed various retention factor values in the range of 0.01 to 0.97. Also, C. lanatus, T. foenum-graecum, and M. koenigii plant leaves confirmed the antioxidant and antidiabetic activity but C. lanatus from Anand city was found to be the best plant. The study will help in the future to identify this plant for further research in industries and pharmaceutical companies.
{"title":"Thin Layer Chromatographic Profiling, Antioxidant and Antidiabetic Activity of Selected Medicinal Plants","authors":"Richa Dodia, Susmita Sahoo","doi":"10.18811/ijpen.v9i02.04","DOIUrl":"https://doi.org/10.18811/ijpen.v9i02.04","url":null,"abstract":"Diabetes mellitus is a disorder whose frequency is speedily increasing everywhere on the planet. According to the World HealthOrganization (WHO), a United Nations Agency, polygenic diseases are going to be the seventh leading reason for death in 2030. Several healthful plants like Citrullus lanatus, Trigonella foenum-graecum, and Murraya koenigii are used for the treatment of diabetes disorders in the Indian medicine system and different ancient systems of the world. These three plant leaves were collected from Vadodara and Anand, Gujarat. Thin layer chromatography profiling of all three plant leaves confirmed the presence of various common classes of phytochemicals such as rutin, gallic acid, tannic acid, and quercetin in both cities. Thin layer chromatographic analysis of a particular leaf revealed various retention factor values in the range of 0.01 to 0.97. Also, C. lanatus, T. foenum-graecum, and M. koenigii plant leaves confirmed the antioxidant and antidiabetic activity but C. lanatus from Anand city was found to be the best plant. The study will help in the future to identify this plant for further research in industries and pharmaceutical companies.","PeriodicalId":14298,"journal":{"name":"INTERNATIONAL JOURNAL OF PLANT AND ENVIRONMENT","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-08-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135620902","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Ganesh D. Bhatt, Mittapalli Bindhupriya, Jenny Salam
Herbal gardens are a rich source of plant-based medicines and their derivatives. Both in urban and rural locations, herbal plants have an important role in traditional medicine. The development of herbal garden includes propagation, multiplication, research, education, and extension activities as well. In the present study, we took herbal plants from different nurseries of Noida and Greater Noida of Gautam Buddha Nagar district, Uttar Pradesh and developed herbal garden in the Galgotias University campus. The herbal garden has approximately 45 different plants, including shrubs (22), trees (13), herbs (9) and grass (1) etc. The geotagged photographs of each herbal plant were captured using GPS map camera. The ex-situ conservation of herbal garden in Galgotias University would help and motivate faculties and students of the university, to undertake further research on medicinal plants.
草药花园是植物性药物及其衍生物的丰富来源。在城市和农村地区，草本植物在传统医学中都发挥着重要作用。草药园林的发展包括繁殖、繁殖、研究、教育和推广活动。在本研究中，我们从北方邦Gautam Buddha Nagar地区的诺伊达和大诺伊达不同的苗圃中提取草药植物，并在Galgotias大学校园内开发了草药花园。草药园大约有45种不同的植物，包括灌木(22种)，树木(13种)，草药(9种)和草(1种)等。使用GPS地图相机拍摄每种草药的地理标记照片。加尔戈塔斯大学草药园的迁地保护将有助于并激励该大学的教师和学生进一步研究药用植物。
{"title":"Establishment of Herbal Garden in the Galgotias University Campus - An Ex-situ Conservation Approach","authors":"Ganesh D. Bhatt, Mittapalli Bindhupriya, Jenny Salam","doi":"10.18811/ijpen.v9i02.06","DOIUrl":"https://doi.org/10.18811/ijpen.v9i02.06","url":null,"abstract":"Herbal gardens are a rich source of plant-based medicines and their derivatives. Both in urban and rural locations, herbal plants have an important role in traditional medicine. The development of herbal garden includes propagation, multiplication, research, education, and extension activities as well. In the present study, we took herbal plants from different nurseries of Noida and Greater Noida of Gautam Buddha Nagar district, Uttar Pradesh and developed herbal garden in the Galgotias University campus. The herbal garden has approximately 45 different plants, including shrubs (22), trees (13), herbs (9) and grass (1) etc. The geotagged photographs of each herbal plant were captured using GPS map camera. The ex-situ conservation of herbal garden in Galgotias University would help and motivate faculties and students of the university, to undertake further research on medicinal plants.","PeriodicalId":14298,"journal":{"name":"INTERNATIONAL JOURNAL OF PLANT AND ENVIRONMENT","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-08-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135620903","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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