Pub Date : 2010-09-01DOI: 10.5145/KJCM.2010.13.3.135
Eun-Sun Jeong, Jong‐Hee Shin, M. Shin, S. Suh, D. Ryang
We report a rare case of fungemia due to Exophiala (Wangiella) dermatitidis in a 4-month-old female infant who was admitted to an intensive care unit with sudden infant death syndrome (SIDS). E. dermatitidiswas repeatedly isolated from blood cultures (on the 28th and 32nd day of hospitalization) of the patient, who died on the 44th day of hospitalization. The fungus was identified by its morphological characteristics and DNA sequencing of both the D1/D2 domain and the ITS region of rDNA. To our knowledge, this is the first reported case of E. dermatitidis fungemia in Korea. (Korean J Clin Microbiol 2010;13: 135-139)
{"title":"Fungemia due to Exophiala dermatitidis","authors":"Eun-Sun Jeong, Jong‐Hee Shin, M. Shin, S. Suh, D. Ryang","doi":"10.5145/KJCM.2010.13.3.135","DOIUrl":"https://doi.org/10.5145/KJCM.2010.13.3.135","url":null,"abstract":"We report a rare case of fungemia due to Exophiala (Wangiella) dermatitidis in a 4-month-old female infant who was admitted to an intensive care unit with sudden infant death syndrome (SIDS). E. dermatitidiswas repeatedly isolated from blood cultures (on the 28th and 32nd day of hospitalization) of the patient, who died on the 44th day of hospitalization. The fungus was identified by its morphological characteristics and DNA sequencing of both the D1/D2 domain and the ITS region of rDNA. To our knowledge, this is the first reported case of E. dermatitidis fungemia in Korea. (Korean J Clin Microbiol 2010;13: 135-139)","PeriodicalId":143093,"journal":{"name":"Korean Journal of Clinical Microbiology","volume":"209 ","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2010-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"132949207","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2010-09-01DOI: 10.5145/KJCM.2010.13.3.128
Youngeun Ma, J. Lee, Seung-Tae Lee, C. Ki, N. Lee
Bacterial morphology can be altered by various factors, including antibiotics. Unusually shaped, large, swollen organisms were observed in a urine culture obtained from a patient who had no history of antibiotic therapy. The organism was identified as Escherichia coli by the Vitek 2 system and by DNA sequencing of 16S rRNA and gyrB. The patient had no symptoms except fever, which subsided without medication. Microbiology laboratories should be aware of the potential appearance of such bacilli to avoid confusion with fungi and other naturally occurring filamentous organisms. (Korean J Clin Microbiol 2010;13:128-131)
{"title":"Aberrant Forms of Escherichia coli in Urine Culture","authors":"Youngeun Ma, J. Lee, Seung-Tae Lee, C. Ki, N. Lee","doi":"10.5145/KJCM.2010.13.3.128","DOIUrl":"https://doi.org/10.5145/KJCM.2010.13.3.128","url":null,"abstract":"Bacterial morphology can be altered by various factors, including antibiotics. Unusually shaped, large, swollen organisms were observed in a urine culture obtained from a patient who had no history of antibiotic therapy. The organism was identified as Escherichia coli by the Vitek 2 system and by DNA sequencing of 16S rRNA and gyrB. The patient had no symptoms except fever, which subsided without medication. Microbiology laboratories should be aware of the potential appearance of such bacilli to avoid confusion with fungi and other naturally occurring filamentous organisms. (Korean J Clin Microbiol 2010;13:128-131)","PeriodicalId":143093,"journal":{"name":"Korean Journal of Clinical Microbiology","volume":"16 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2010-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"124645382","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2010-09-01DOI: 10.5145/KJCM.2010.13.3.121
S. M. Hwang, I. Yoon, S. Joo, Jongyoun Yi, E. Kim
Genital mycoplasmas are rare in extraintestinal specimens, but can cause disseminated infections in immunocompromised patients and wound infections after surgery or injury. We report two cases of Myoplasma hominis wound infections after lung lobectomy and kidney transplantation, and a case of M. salivarium wound infection after aortic graft replacement. Mycoplasmas grew in aerobic and anaerobic cultures as tiny colonies but were not observed by gram- or acid fast stain and were confirmed by MYCOFAST EvolutioN 2 kit or 16S rRNA sequencing. These cases indicated that mycoplasmas were probably underestimated in wound infections because they were not in suspicion. We suggest that Mycoplasma should be suspected when microorganisms are not readily observable in Gram stains but can be cultured. (Korean J Clin Microbiol 2010;13: 121-124)
{"title":"Importance of Suspicion for the Identification of Mycoplasma in Wound Culture: A Case Report","authors":"S. M. Hwang, I. Yoon, S. Joo, Jongyoun Yi, E. Kim","doi":"10.5145/KJCM.2010.13.3.121","DOIUrl":"https://doi.org/10.5145/KJCM.2010.13.3.121","url":null,"abstract":"Genital mycoplasmas are rare in extraintestinal specimens, but can cause disseminated infections in immunocompromised patients and wound infections after surgery or injury. We report two cases of Myoplasma hominis wound infections after lung lobectomy and kidney transplantation, and a case of M. salivarium wound infection after aortic graft replacement. Mycoplasmas grew in aerobic and anaerobic cultures as tiny colonies but were not observed by gram- or acid fast stain and were confirmed by MYCOFAST EvolutioN 2 kit or 16S rRNA sequencing. These cases indicated that mycoplasmas were probably underestimated in wound infections because they were not in suspicion. We suggest that Mycoplasma should be suspected when microorganisms are not readily observable in Gram stains but can be cultured. (Korean J Clin Microbiol 2010;13: 121-124)","PeriodicalId":143093,"journal":{"name":"Korean Journal of Clinical Microbiology","volume":"13 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2010-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130393751","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2010-09-01DOI: 10.5145/KJCM.2010.13.3.144
Sunjoo Kim
{"title":"Registration of KJCM on the Journal List of 'National Research Foundation'","authors":"Sunjoo Kim","doi":"10.5145/KJCM.2010.13.3.144","DOIUrl":"https://doi.org/10.5145/KJCM.2010.13.3.144","url":null,"abstract":"","PeriodicalId":143093,"journal":{"name":"Korean Journal of Clinical Microbiology","volume":"36 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2010-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"123939963","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2010-06-01DOI: 10.5145/KJCM.2010.13.2.53
S. Jun, Kee-Hyung Sung, S. Song, K. Park, Hong-Bin Kim, Junghan Song, E. Choi, S. Park, E. Kim
Background: Enteroviruses are the most frequent etiologic agents of aseptic meningitis and are estimated to be the cause of 70% to 90% of viral meningitis cases. Enterovirus diagnosis can be difficult because clinical features vary according to patient immunity and age. The purpose of this study was to evaluate the performance of the real-time nucleic acid sequence-based amplification (NASBA) assay compared to that of the real-time nested RT-PCR assay for enterovirus detection. Methods: This study was performed on 96 patients suspected of aseptic meningitis based on clinical features. RNA was extracted using NucliSENS EasyMAG and real-time NASBA assay was performed using NucliSENS EasyQ Enterovirus and NucliSENS EasyQ Basic 2. We also executed in-house real-time nested RT-PCR assay for RNA extracted via QIAamp Viral RNA Mini. Results: The positive rate of real-time NASBA assay was 45.8% for enterovirus detection. The positive rate of first real-time reverse transcription PCR was 22.9% and the second real-time PCR was 57.3%. The concordant rate of the real-time NASBA assay and first real-time reverse transcription PCR was 75.0%. The concordant rate of the real-time NASBA assay and second real-time PCR was 86.5%. Conclusion: The detection of enteroviruses using the real-time NASBA assay is less prone to cross-contamination and is simple, without the need for reverse transcription. We conclude that the NASBA assay is an effective method for the rapid diagnosis of aseptic meningitis. (Korean J Clin Microbiol 2010;13: 53-58)
{"title":"Detection of Enterovirus using Real-Time Nucleic Acid Sequence-based Amplification.","authors":"S. Jun, Kee-Hyung Sung, S. Song, K. Park, Hong-Bin Kim, Junghan Song, E. Choi, S. Park, E. Kim","doi":"10.5145/KJCM.2010.13.2.53","DOIUrl":"https://doi.org/10.5145/KJCM.2010.13.2.53","url":null,"abstract":"Background: Enteroviruses are the most frequent etiologic agents of aseptic meningitis and are estimated to be the cause of 70% to 90% of viral meningitis cases. Enterovirus diagnosis can be difficult because clinical features vary according to patient immunity and age. The purpose of this study was to evaluate the performance of the real-time nucleic acid sequence-based amplification (NASBA) assay compared to that of the real-time nested RT-PCR assay for enterovirus detection. Methods: This study was performed on 96 patients suspected of aseptic meningitis based on clinical features. RNA was extracted using NucliSENS EasyMAG and real-time NASBA assay was performed using NucliSENS EasyQ Enterovirus and NucliSENS EasyQ Basic 2. We also executed in-house real-time nested RT-PCR assay for RNA extracted via QIAamp Viral RNA Mini. Results: The positive rate of real-time NASBA assay was 45.8% for enterovirus detection. The positive rate of first real-time reverse transcription PCR was 22.9% and the second real-time PCR was 57.3%. The concordant rate of the real-time NASBA assay and first real-time reverse transcription PCR was 75.0%. The concordant rate of the real-time NASBA assay and second real-time PCR was 86.5%. Conclusion: The detection of enteroviruses using the real-time NASBA assay is less prone to cross-contamination and is simple, without the need for reverse transcription. We conclude that the NASBA assay is an effective method for the rapid diagnosis of aseptic meningitis. (Korean J Clin Microbiol 2010;13: 53-58)","PeriodicalId":143093,"journal":{"name":"Korean Journal of Clinical Microbiology","volume":"47 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2010-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127197238","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2010-06-01DOI: 10.5145/KJCM.2010.13.2.68
Kyung-Hee Kim, Jung-eun Kim, S. Park, Y. Song, J. Ahn, P. Park, Y. Seo
Background: In January 2008, the Clinical and Laboratory Standards Institute (CLSI) published revised penicillin breakpoints for Streptococcus pneumoniae according to clinical presentation and the route of penicillin administration. The aim of this study was to evaluate the impacts of the new penicillin breakpoints on the susceptibility rates of S. pneumoniae isolated from blood. Methods: A total of 156 non-duplicated S. pneumoniae strains recovered from blood of hospitalized patients were collected between January 2003 and December 2008. Penicillin and cefotaxime susceptibility tests were performed using an E-test (AB Biodisk, Solna, Sweden). Results of the penicillin susceptibility tests were analyzed using the former and new CLSI guidelines. Results: Of the 156 S. pneumoniae strains isolated from blood, penicillin susceptibility under the former CLSI guidelines resulted in 42.3% susceptible, 42.3% intermediate, and 15.4% resistant states. According to the new CLSI guidelines (nonmeningitis, parenteral), 87.8% of isolates were susceptible, 9.6% were intermediate, and 2.6% were resistant to penicillin. Conclusion: When the new CLSI guidelines are applied, the penicillin susceptibility rate of S. pneumoniae strains isolated from blood is considerably increased. This suggests that penicillin should still be useful for the treatment of nonmeningeal pneumococcal infections and that the use of broad-spectrum antimicrobials should not replace this treatment. (Korean J Clin Microbiol 2010;13:68-72)
背景:2008年1月，临床与实验室标准协会(CLSI)根据临床表现和青霉素给药途径发布了修订后的肺炎链球菌青霉素断点。本研究的目的是评估新的青霉素断点对从血液分离的肺炎链球菌的敏感性的影响。方法:收集2003年1月~ 2008年12月住院患者血液中分离的非重复肺炎链球菌156株。采用E-test (AB Biodisk，索尔纳，瑞典)进行青霉素和头孢噻肟药敏试验。青霉素药敏试验结果分析采用旧的和新的CLSI指南。结果:从血液中分离出的156株肺炎链球菌中，在原CLSI指南下的青霉素敏感性为42.3%为敏感，42.3%为中间状态，15.4%为耐药状态。根据新的CLSI指南(非脑膜炎，肠外注射)，87.8%的分离株对青霉素敏感，9.6%为中间，2.6%为耐药。结论:应用新的CLSI指南后，血源肺炎链球菌的青霉素敏感性明显提高。这表明，青霉素仍可用于治疗非脑膜肺炎球菌感染，广谱抗菌药物不应取代这种治疗方法。(韩国临床微生物学杂志2010;13:68-72)
{"title":"Impact of Revised Penicillin Breakpoints for Streptococcus pneumoniae (CLSI M100-S18) on the Penicillin Susceptibility Rate","authors":"Kyung-Hee Kim, Jung-eun Kim, S. Park, Y. Song, J. Ahn, P. Park, Y. Seo","doi":"10.5145/KJCM.2010.13.2.68","DOIUrl":"https://doi.org/10.5145/KJCM.2010.13.2.68","url":null,"abstract":"Background: In January 2008, the Clinical and Laboratory Standards Institute (CLSI) published revised penicillin breakpoints for Streptococcus pneumoniae according to clinical presentation and the route of penicillin administration. The aim of this study was to evaluate the impacts of the new penicillin breakpoints on the susceptibility rates of S. pneumoniae isolated from blood. Methods: A total of 156 non-duplicated S. pneumoniae strains recovered from blood of hospitalized patients were collected between January 2003 and December 2008. Penicillin and cefotaxime susceptibility tests were performed using an E-test (AB Biodisk, Solna, Sweden). Results of the penicillin susceptibility tests were analyzed using the former and new CLSI guidelines. Results: Of the 156 S. pneumoniae strains isolated from blood, penicillin susceptibility under the former CLSI guidelines resulted in 42.3% susceptible, 42.3% intermediate, and 15.4% resistant states. According to the new CLSI guidelines (nonmeningitis, parenteral), 87.8% of isolates were susceptible, 9.6% were intermediate, and 2.6% were resistant to penicillin. Conclusion: When the new CLSI guidelines are applied, the penicillin susceptibility rate of S. pneumoniae strains isolated from blood is considerably increased. This suggests that penicillin should still be useful for the treatment of nonmeningeal pneumococcal infections and that the use of broad-spectrum antimicrobials should not replace this treatment. (Korean J Clin Microbiol 2010;13:68-72)","PeriodicalId":143093,"journal":{"name":"Korean Journal of Clinical Microbiology","volume":"200 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2010-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"114876109","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2010-06-01DOI: 10.5145/KJCM.2010.13.2.85
Haewol Cho, M. Cho, Shin-Ae Noh, M. N. Kim, Kyoung Mo Kim
Enterohemorrhagic Escherichia coli (EHEC) is an important cause of bloody diarrhea in children, but is considered to be rare in infants. Herein, a case of infant hemorrhagic colitis of verotoxin-producing E. coli O157:H7 diagnosed by multiplex PCR is reported. A nine-month-old boy was admitted to our hospital with bloody diarrhea for the previous two days. Multiplex PCR using SeeplexR Diarrhea ACE Detection Kit (Seegene, Seoul, Korea) was directly applied to the stool specimens. Amplified bands specific for verotoxin, O157, and H7 indicated the presence of O157:H7 EHEC. The stool specimens were inoculated on sorbitol-MacConkey agar (SMA) and tryptic soy broth containing mitomycin C (TSB-M). Colorless colonies on sorbitol-MacConkey agar were O157-positive. TSB-M enrichment cultures of the stool specimen and the isolates were positive for verotoxin according to an enzyme immunoassay (EIA). The prepared ingredients of baby foods for the patient including ground meat, chopped carrot, chopped cabbage, and white rice porridge showed no EHEC on TSB-M and SMA. The patient’s parents and three-year-old sister did not recently have any gastrointestinal symptoms. Cefdinir was administered for one day and was ceased after diagnosis of EHEC colitis. The stool culture and verotoxin assay were negative on the second day of hospitalization. Application of multiplex PCR and verotoxin EIA directly to diarrheal stool warrants the rapid diagnosis and appropriate treatment of EHEC colitis. (Korean J Clin Microbiol 2010;13:85-89)
{"title":"A Case of Verotoxin-producing Escherichia coli O157:H7 with Hemorrhagic Colitis in an Infant, Diagnosed by Multiplex PCR","authors":"Haewol Cho, M. Cho, Shin-Ae Noh, M. N. Kim, Kyoung Mo Kim","doi":"10.5145/KJCM.2010.13.2.85","DOIUrl":"https://doi.org/10.5145/KJCM.2010.13.2.85","url":null,"abstract":"Enterohemorrhagic Escherichia coli (EHEC) is an important cause of bloody diarrhea in children, but is considered to be rare in infants. Herein, a case of infant hemorrhagic colitis of verotoxin-producing E. coli O157:H7 diagnosed by multiplex PCR is reported. A nine-month-old boy was admitted to our hospital with bloody diarrhea for the previous two days. Multiplex PCR using SeeplexR Diarrhea ACE Detection Kit (Seegene, Seoul, Korea) was directly applied to the stool specimens. Amplified bands specific for verotoxin, O157, and H7 indicated the presence of O157:H7 EHEC. The stool specimens were inoculated on sorbitol-MacConkey agar (SMA) and tryptic soy broth containing mitomycin C (TSB-M). Colorless colonies on sorbitol-MacConkey agar were O157-positive. TSB-M enrichment cultures of the stool specimen and the isolates were positive for verotoxin according to an enzyme immunoassay (EIA). The prepared ingredients of baby foods for the patient including ground meat, chopped carrot, chopped cabbage, and white rice porridge showed no EHEC on TSB-M and SMA. The patient’s parents and three-year-old sister did not recently have any gastrointestinal symptoms. Cefdinir was administered for one day and was ceased after diagnosis of EHEC colitis. The stool culture and verotoxin assay were negative on the second day of hospitalization. Application of multiplex PCR and verotoxin EIA directly to diarrheal stool warrants the rapid diagnosis and appropriate treatment of EHEC colitis. (Korean J Clin Microbiol 2010;13:85-89)","PeriodicalId":143093,"journal":{"name":"Korean Journal of Clinical Microbiology","volume":"41 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2010-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131266495","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2010-06-01DOI: 10.5145/KJCM.2010.13.2.98
Hae-Sun Chung, J. Lee, H. Kim, N. Lee
Otorhinolaryngology-Head and Neck Surgery,Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, KoreaAllergic fungal sinusitis (AFS) is a noninvasive form of fungal rhinosinusitis resulting from an IgE-medi-ated hypersensitivity reaction. The diagnosis of AFS can be established by demonstrating type I hyper-sensitivity, presence of fungus in mucus, eosinophilic mucin, nasal polyposis, and characteristic CT scans. Although AFS is not unusual and its incidence may be increasing, few cases have been reported in Korea. Here, we report the first case of typical AFS in which
{"title":"A Case of Allergic Fungal Sinusitis due to Curvularia","authors":"Hae-Sun Chung, J. Lee, H. Kim, N. Lee","doi":"10.5145/KJCM.2010.13.2.98","DOIUrl":"https://doi.org/10.5145/KJCM.2010.13.2.98","url":null,"abstract":"Otorhinolaryngology-Head and Neck Surgery,Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, KoreaAllergic fungal sinusitis (AFS) is a noninvasive form of fungal rhinosinusitis resulting from an IgE-medi-ated hypersensitivity reaction. The diagnosis of AFS can be established by demonstrating type I hyper-sensitivity, presence of fungus in mucus, eosinophilic mucin, nasal polyposis, and characteristic CT scans. Although AFS is not unusual and its incidence may be increasing, few cases have been reported in Korea. Here, we report the first case of typical AFS in which","PeriodicalId":143093,"journal":{"name":"Korean Journal of Clinical Microbiology","volume":"43 1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2010-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"123638578","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2010-06-01DOI: 10.5145/KJCM.2010.13.2.93
A. R. Cho, H. Kim, Mi-Kyung Lee, Seong-Ho Choi, S. Yun
We report a case of catheter-related bacteremia due to Tsukamurella pulmonis. T. pulmonis is a rare cause of opportunistic infection in immunosuppressed patients and in cases of indwelling foreign materials. This infection was nearly impossible to identify using conventional phenotyping methods because of its similarities to the related genera Nocardia, Rhodococcus, Gordonia, Streptomyces, Corynebacterium, and Mycobacterium. This organism was initially misidentified as Mycobacterium aubagnense through PCR-RFLP analysis. We correctly identified this organism using 16S rRNA sequencing combined with phenotyping tests. (Korean J Clin Microbiol 2010;13: 93-97)
{"title":"A Case Report of Tsukamurella pulmonis Infection Misidentified as Atypical Mycobacteria","authors":"A. R. Cho, H. Kim, Mi-Kyung Lee, Seong-Ho Choi, S. Yun","doi":"10.5145/KJCM.2010.13.2.93","DOIUrl":"https://doi.org/10.5145/KJCM.2010.13.2.93","url":null,"abstract":"We report a case of catheter-related bacteremia due to Tsukamurella pulmonis. T. pulmonis is a rare cause of opportunistic infection in immunosuppressed patients and in cases of indwelling foreign materials. This infection was nearly impossible to identify using conventional phenotyping methods because of its similarities to the related genera Nocardia, Rhodococcus, Gordonia, Streptomyces, Corynebacterium, and Mycobacterium. This organism was initially misidentified as Mycobacterium aubagnense through PCR-RFLP analysis. We correctly identified this organism using 16S rRNA sequencing combined with phenotyping tests. (Korean J Clin Microbiol 2010;13: 93-97)","PeriodicalId":143093,"journal":{"name":"Korean Journal of Clinical Microbiology","volume":"10 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2010-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126902080","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2010-06-01DOI: 10.5145/KJCM.2010.13.2.90
H. Huh, Seung-Tae Lee, J. Lee, C. Ki, N. Lee
Clostridium symbiosum was isolated from the blood of a 61-year-old immunocompromised woman who had diagnosed ovarian cancer with multiple metastases and who had developed persistent tachycardia. A blood culture was drawn from the peripherally inserted central catheter, and non-spore-forming gram-negative rods were detected in an anaerobic vial. The organism showed tiny and pinpoint colonies and was unidentified by Vitek II (bioMerieux, France). The 16S rRNA gene sequence showed a 99.4% identity with C. symbiosum. To our knowledge, this represents the first report of C. symbiosum isolation in Korea. (Korean J Clin Microbiol 2010;13: 90-92)
从一名61岁的免疫功能低下妇女的血液中分离出共生梭菌，该妇女被诊断出患有多发性转移的卵巢癌，并出现了持续性心动过速。从周围插入的中心导管中进行血培养，在厌氧瓶中检测非孢子形成的革兰氏阴性棒。该生物显示出微小和精确的菌落，并由Vitek II (bioMerieux, France)鉴定。16S rRNA基因序列与共生菌的同源性为99.4%。据我们所知，这是韩国首次报道的共生菌分离。(中华临床微生物学杂志2010;13:90-92)
{"title":"Clostridium symbiosum Isolated from Blood","authors":"H. Huh, Seung-Tae Lee, J. Lee, C. Ki, N. Lee","doi":"10.5145/KJCM.2010.13.2.90","DOIUrl":"https://doi.org/10.5145/KJCM.2010.13.2.90","url":null,"abstract":"Clostridium symbiosum was isolated from the blood of a 61-year-old immunocompromised woman who had diagnosed ovarian cancer with multiple metastases and who had developed persistent tachycardia. A blood culture was drawn from the peripherally inserted central catheter, and non-spore-forming gram-negative rods were detected in an anaerobic vial. The organism showed tiny and pinpoint colonies and was unidentified by Vitek II (bioMerieux, France). The 16S rRNA gene sequence showed a 99.4% identity with C. symbiosum. To our knowledge, this represents the first report of C. symbiosum isolation in Korea. (Korean J Clin Microbiol 2010;13: 90-92)","PeriodicalId":143093,"journal":{"name":"Korean Journal of Clinical Microbiology","volume":"13 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2010-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122601247","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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