Pub Date : 2010-06-01DOI: 10.5145/KJCM.2010.13.2.79
Tae-Hyoung Kim, Y. S. Lee, Mi-Kyung Lee, K. Lee
Background: Candida species are the fourth leading cause of nosocomial bloodstream infections and have one of the highest mortality rates among nosocomial pathogens. C. tropicalis has been reported to be one of the leading Candida species other than C. albicans to cause Candida infection in patients who have malignancy, diabetes mellitus, and burn. This study was designed to determine whether burn might influence the species distribution and susceptibilities of azoles against clinical isolates of Candida species including C. tropicalis. Methods: A total 372 Candida isolates from various samples in a tertiary burn center were studied, and the MICs of Candida isolates to fluconazole, itraconazole, and voriconazole were tested by broth microdilution method of the Clinical and Laboratory Standards Institute (CLSI) M27-A2. A comparison was made between Candida isolates from burn patients and non-burn patients. Results: The percentages of C. albicans, C. tropicalis, C. parapsilosis and C. glabrata isolates from burn patients and non-burn patients were 42.3% and 64.2% (P=0.000), 35.7% and 21.6% (P=0.002), 11.9% and 7.8%, and 10.1% and 6.4%, respectively. Decreased susceptibilities to fluconazole, itraconazole, and voriconazole were observed more frequently in burn patients (4.76%, 19.05%, and 0.60%, respectively) than non-burn patients (2.45%, 14.22%, and 0%, respectively). Conclusion: The results of this study suggest that burn may lead to influence the species distribution and susceptibilities to azoles of Candida species. (Korean J Clin Microbiol 2010;13:79-84)
{"title":"Species Distribution and Susceptibilities to Azoles of Candida Species Including C. tropicalis in a Tertiary Burn Center","authors":"Tae-Hyoung Kim, Y. S. Lee, Mi-Kyung Lee, K. Lee","doi":"10.5145/KJCM.2010.13.2.79","DOIUrl":"https://doi.org/10.5145/KJCM.2010.13.2.79","url":null,"abstract":"Background: Candida species are the fourth leading cause of nosocomial bloodstream infections and have one of the highest mortality rates among nosocomial pathogens. C. tropicalis has been reported to be one of the leading Candida species other than C. albicans to cause Candida infection in patients who have malignancy, diabetes mellitus, and burn. This study was designed to determine whether burn might influence the species distribution and susceptibilities of azoles against clinical isolates of Candida species including C. tropicalis. Methods: A total 372 Candida isolates from various samples in a tertiary burn center were studied, and the MICs of Candida isolates to fluconazole, itraconazole, and voriconazole were tested by broth microdilution method of the Clinical and Laboratory Standards Institute (CLSI) M27-A2. A comparison was made between Candida isolates from burn patients and non-burn patients. Results: The percentages of C. albicans, C. tropicalis, C. parapsilosis and C. glabrata isolates from burn patients and non-burn patients were 42.3% and 64.2% (P=0.000), 35.7% and 21.6% (P=0.002), 11.9% and 7.8%, and 10.1% and 6.4%, respectively. Decreased susceptibilities to fluconazole, itraconazole, and voriconazole were observed more frequently in burn patients (4.76%, 19.05%, and 0.60%, respectively) than non-burn patients (2.45%, 14.22%, and 0%, respectively). Conclusion: The results of this study suggest that burn may lead to influence the species distribution and susceptibilities to azoles of Candida species. (Korean J Clin Microbiol 2010;13:79-84)","PeriodicalId":143093,"journal":{"name":"Korean Journal of Clinical Microbiology","volume":"14 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2010-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"114372056","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2010-06-01DOI: 10.5145/KJCM.2010.13.2.59
Kyung-Sun Park, M. Kim, T. Park, J. Suh, Hee-Joo Lee
Background: In Korea, a sudden increase in vancomycin-resistant enterococci (VRE) infection has been noted since the late 1990s. This study was conducted to describe the antimicrobial resistances of enterococcal blood isolates and to identify risk factors associated with VRE bacteremia in a tertiary care university hospital over a recent five-year period. Methods: This study was conducted to analyze the antimicrobial susceptibilities of enterococcal blood isolates by year from January 2003 to December 2007. Multivariate logistic regression analysis was used to investigate factors associated with VRE bacteremia. Results: A total of 225 enterococcal strains (44.7% Enterococcus faecalis, 42.4% Enterococcus facium, 5.9% Enterococcus casseliflavus, and 4.7% Enterococcus gallinarum) were detected in blood, 55 of which (21.6%) were resistant to vancomycin. In 2004 and 2005, the resistance rates for vancomycin and teicoplanin (33.3% and 27.3%; 34.4% and 23.0%, respectively) increased. In 2003, 2006, and 2007, the resistance rates for vancomycin and teicoplanin (8.7% and 8.7%; 19.0% and 14.3%; 13.5% and 11.5%, respectively) decreased relative to those of the previous years. When 55 patients with VRE bacteremia were compared with 55 patients with vancomycin-susceptible enterococcal bacteremia using multivariate analysis, E. faecium bacteremia (OR 12.624, P＜0.001) and enterococcal bacteremia caused by species other than E. faecium and E. faecalis (OR 21.473, P=0.011) were found to be statistical risk factors. Among several infection control activities, the restricted uses of vancomycin and quinupristin-dalfopristin decreased the vancomycin resistance rate from 27.78% to 15.50% (P=0.0257). Conclusion: VRE bacteremia would be effectively controlled via infection control activities based on studies regarding risk factors associated with VRE bacteremia. (Korean J Clin Microbiol 2010;13:59-67)
{"title":"Antimicrobial Resistance of Enterococcal Isolates from Blood and Risk Factors for Vancomycin Resistant Enterococcal Bacteremia in a Tertiary Care University Hospital from 2003 to 2007","authors":"Kyung-Sun Park, M. Kim, T. Park, J. Suh, Hee-Joo Lee","doi":"10.5145/KJCM.2010.13.2.59","DOIUrl":"https://doi.org/10.5145/KJCM.2010.13.2.59","url":null,"abstract":"Background: In Korea, a sudden increase in vancomycin-resistant enterococci (VRE) infection has been noted since the late 1990s. This study was conducted to describe the antimicrobial resistances of enterococcal blood isolates and to identify risk factors associated with VRE bacteremia in a tertiary care university hospital over a recent five-year period. Methods: This study was conducted to analyze the antimicrobial susceptibilities of enterococcal blood isolates by year from January 2003 to December 2007. Multivariate logistic regression analysis was used to investigate factors associated with VRE bacteremia. Results: A total of 225 enterococcal strains (44.7% Enterococcus faecalis, 42.4% Enterococcus facium, 5.9% Enterococcus casseliflavus, and 4.7% Enterococcus gallinarum) were detected in blood, 55 of which (21.6%) were resistant to vancomycin. In 2004 and 2005, the resistance rates for vancomycin and teicoplanin (33.3% and 27.3%; 34.4% and 23.0%, respectively) increased. In 2003, 2006, and 2007, the resistance rates for vancomycin and teicoplanin (8.7% and 8.7%; 19.0% and 14.3%; 13.5% and 11.5%, respectively) decreased relative to those of the previous years. When 55 patients with VRE bacteremia were compared with 55 patients with vancomycin-susceptible enterococcal bacteremia using multivariate analysis, E. faecium bacteremia (OR 12.624, P＜0.001) and enterococcal bacteremia caused by species other than E. faecium and E. faecalis (OR 21.473, P=0.011) were found to be statistical risk factors. Among several infection control activities, the restricted uses of vancomycin and quinupristin-dalfopristin decreased the vancomycin resistance rate from 27.78% to 15.50% (P=0.0257). Conclusion: VRE bacteremia would be effectively controlled via infection control activities based on studies regarding risk factors associated with VRE bacteremia. (Korean J Clin Microbiol 2010;13:59-67)","PeriodicalId":143093,"journal":{"name":"Korean Journal of Clinical Microbiology","volume":"265 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2010-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"133287112","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2010-06-01DOI: 10.5145/KJCM.2010.13.2.73
M. Kim, Won-Kil Lee
Background: Chlamydophila pneumoniae is one of the major respiratory infectious pathogens and can be accurately diagnosed by cell culturing. The author performed this study to compare the usefulness of the collagen-coated polyethylene terephthalate (PET) disc culture method and that of the shell vial method. Methods: Twenty-nine sputums and 17 blood specimens collected from 46 patients for C. pneumoniae culture were inoculated into HeLa-229 cell monolayers cultured in shell vials and polyester plates. After incubation, they were stained using the indirect immunofluorescent method with genus-specific FITC-conjugated anti-chlamydia antibody. When both results were inconsistent, microimmunofluorescence results were used. Results: HeLa-229 cells successfully formed monolayers in shell vials and collagen-coated PET plates in all cases. Positive inclusion bodies in HeLa-229 cells of shell vials and PET plates for C. pneumoniae culture were similarly stained with the indirect immunofluorescent method. Both methods showed consistent results with 20 positive and 22 negative cases. The total agreement between the PET plate and shell vial was excellent (91.3%, k=0.826). Conclusion: The collagen-coated PET disc culture method showed highly consistent results with that of the shell vial method, and no technical differences were experienced between the two methods. Therefore, the author concluded that the shell vial method could be replaced by the PET plate method for detection of C. pneumoniae. (Korean J Clin Microbiol 2010;13:73-78)
{"title":"Comparison of Collagen-coated Polyethylene Terephthalate Disc Plate and Shell Vial Culture Method for the Isolation of Chlamydophila pneumoniae","authors":"M. Kim, Won-Kil Lee","doi":"10.5145/KJCM.2010.13.2.73","DOIUrl":"https://doi.org/10.5145/KJCM.2010.13.2.73","url":null,"abstract":"Background: Chlamydophila pneumoniae is one of the major respiratory infectious pathogens and can be accurately diagnosed by cell culturing. The author performed this study to compare the usefulness of the collagen-coated polyethylene terephthalate (PET) disc culture method and that of the shell vial method. Methods: Twenty-nine sputums and 17 blood specimens collected from 46 patients for C. pneumoniae culture were inoculated into HeLa-229 cell monolayers cultured in shell vials and polyester plates. After incubation, they were stained using the indirect immunofluorescent method with genus-specific FITC-conjugated anti-chlamydia antibody. When both results were inconsistent, microimmunofluorescence results were used. Results: HeLa-229 cells successfully formed monolayers in shell vials and collagen-coated PET plates in all cases. Positive inclusion bodies in HeLa-229 cells of shell vials and PET plates for C. pneumoniae culture were similarly stained with the indirect immunofluorescent method. Both methods showed consistent results with 20 positive and 22 negative cases. The total agreement between the PET plate and shell vial was excellent (91.3%, k=0.826). Conclusion: The collagen-coated PET disc culture method showed highly consistent results with that of the shell vial method, and no technical differences were experienced between the two methods. Therefore, the author concluded that the shell vial method could be replaced by the PET plate method for detection of C. pneumoniae. (Korean J Clin Microbiol 2010;13:73-78)","PeriodicalId":143093,"journal":{"name":"Korean Journal of Clinical Microbiology","volume":"35 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2010-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121328755","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2010-03-01DOI: 10.5145/KJCM.2010.13.1.14
In‐Suk Kim, Eun-Ha Koh, Sunjoo Kim, Kook-Young Maeng, H. Jung
Background: The Streptococcus pneumoniae urinary antigen test (SPUAT) (Binax Now, USA) was developed for detecting polysaccharide C in urine samples for rapid diagnosis of pneumococcal pneumonia, the most common cause of community-acquired pneumonia (CAP). To validate positive results of these tests, we retrospectively investigated all positive results obtained from the emergency room of a Korean university hospital among patients with suspected CAP. Methods: One hundred twenty-three positive SPUAT results were abstracted and analyzed from the authors' laboratory information system among the SPUAT results performed from 1,143 pneumonic patients admitted from the emergency room of a university hospital between 2007 and 2008. Medical records, including conventional microbiologic analysis results, were reviewed in detail for all positive test results. Results: Among 123 patients with the positive SPUAT results, 24 patients were excluded due to hospitalization history during the preceding month. Nine of 99 patients (9.1%) with suspected CAP had confirmed pneumococcal pneumonia upon conventional sputum or blood culture. Thirty-five positive results (35.4%) showed other microorganisms upon conventional methods, which might be due to possible cross-reactivity. Among those, 23 positive results were considered bacterial pneumonic agents, and 12 positive results were regarded as urinary tract infection strains or contaminating agents. Fifty-five positive SPUAT results (55.6%) showed negative conventional microbiologic growth, and some positive SPUAT results might be caused by true pneumococcal infection although without cultural evidence. Conclusion: Our retrospective study demonstrated that a positive SPUAT result typically does not agree well with conventional culture methods, suggesting that the value of a positive SPUAT result in etiology determination may be limited under practical conditions in a university hospital. (Korean J Clin Microbiol 2010;13:14-18)
{"title":"Investigation of Positive Streptococcus pneumoniae Urinary Antigen Test Results in a Korean University Hospital","authors":"In‐Suk Kim, Eun-Ha Koh, Sunjoo Kim, Kook-Young Maeng, H. Jung","doi":"10.5145/KJCM.2010.13.1.14","DOIUrl":"https://doi.org/10.5145/KJCM.2010.13.1.14","url":null,"abstract":"Background: The Streptococcus pneumoniae urinary antigen test (SPUAT) (Binax Now, USA) was developed for detecting polysaccharide C in urine samples for rapid diagnosis of pneumococcal pneumonia, the most common cause of community-acquired pneumonia (CAP). To validate positive results of these tests, we retrospectively investigated all positive results obtained from the emergency room of a Korean university hospital among patients with suspected CAP. Methods: One hundred twenty-three positive SPUAT results were abstracted and analyzed from the authors' laboratory information system among the SPUAT results performed from 1,143 pneumonic patients admitted from the emergency room of a university hospital between 2007 and 2008. Medical records, including conventional microbiologic analysis results, were reviewed in detail for all positive test results. Results: Among 123 patients with the positive SPUAT results, 24 patients were excluded due to hospitalization history during the preceding month. Nine of 99 patients (9.1%) with suspected CAP had confirmed pneumococcal pneumonia upon conventional sputum or blood culture. Thirty-five positive results (35.4%) showed other microorganisms upon conventional methods, which might be due to possible cross-reactivity. Among those, 23 positive results were considered bacterial pneumonic agents, and 12 positive results were regarded as urinary tract infection strains or contaminating agents. Fifty-five positive SPUAT results (55.6%) showed negative conventional microbiologic growth, and some positive SPUAT results might be caused by true pneumococcal infection although without cultural evidence. Conclusion: Our retrospective study demonstrated that a positive SPUAT result typically does not agree well with conventional culture methods, suggesting that the value of a positive SPUAT result in etiology determination may be limited under practical conditions in a university hospital. (Korean J Clin Microbiol 2010;13:14-18)","PeriodicalId":143093,"journal":{"name":"Korean Journal of Clinical Microbiology","volume":"109 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2010-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"124067062","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2010-03-01DOI: 10.5145/KJCM.2010.13.1.27
K. Roh, Chang Ki Kim, J. Yum, D. Yong, S. Jeong, C. Lim, C. Lee, Yunjung Cho, Kyungwon Lee, Y. Chong
Background: Increasing numbers of Acinetobacter spp. resistant to multiple drugs, including carbape- nem, has been a serious problem. The aims of this study were to determine carbapenem resistance pat- terns and mechanisms, as well as to study the mo- lecular epidemiology of Acinetobacter spp. Methods: Clinical isolates of Acinetobacter spp. were collected from May to November in 2006. Antimicro- bial susceptibility testing was performed using CLSI disk diffusion and agar dilution methods. Metallo-β- lactamase- and OXA carbapenemase-producing iso- lates were detected by PCR. Carbapenem resistance and hydrolytic activities were compared according to OXA type and presence of ISAba1. Pulsed-field gel electrophoresis (PFGE) was performed to determine the epidemiologic features. Results: The imipenem non-susceptible rates were variable from 10% to 67%. Among 151 isolates car- rying blaOXA-51-like, 75 isolates carried both blaOXA-51-like and ISAba1, and 25 isolates had both blaOXA-51-like, blaOXA-23-like, and ISAba1. Carbapenem MICs of both blaOXA-51-like and ISAba1-carrying isolates were higher than those with blaOXA-51-like only. Carbapenem MICs of blaOXA-23-like-carrying isolates were higher than those with both blaOXA-51-like and ISAba1. Both blaOXA-51-like and ISAba1-carrying isolates and blaOXA-51-like, blaOXA-23-like, and ISAba1-carrying isolates demonstrated higher hydrolysis activities in oxacillin and carbapenems. Most of the tested isolates were susceptible to tige- cycline, and all of them were susceptible to colistin. Pulsed-field gel electrophoresis suggested that there had been several outbreaks of blaOXA-23-like and blaOXA-51-like-positive strains. Conclusion: Carbapenem non-susceptible Acineto- bacter isolates and OXA carbapenemase-producing isolates were prevalent. Dissemination of blaOXA-har- boring isolates may make it difficult to treat infections due to carbapenem-resistant Acinetobacter spp. Further surveillance studies are required to prevent the spread of carbapenem resistance. (Korean J Clin Microbiol 2010;13:27-33)
{"title":"Carbapenem Resistance Mechanisms and Molecular Epidemiology of Acinetobacter spp. from Four Hospitals in Seoul and Gyeonggi Province in 2006","authors":"K. Roh, Chang Ki Kim, J. Yum, D. Yong, S. Jeong, C. Lim, C. Lee, Yunjung Cho, Kyungwon Lee, Y. Chong","doi":"10.5145/KJCM.2010.13.1.27","DOIUrl":"https://doi.org/10.5145/KJCM.2010.13.1.27","url":null,"abstract":"Background: Increasing numbers of Acinetobacter spp. resistant to multiple drugs, including carbape- nem, has been a serious problem. The aims of this study were to determine carbapenem resistance pat- terns and mechanisms, as well as to study the mo- lecular epidemiology of Acinetobacter spp. Methods: Clinical isolates of Acinetobacter spp. were collected from May to November in 2006. Antimicro- bial susceptibility testing was performed using CLSI disk diffusion and agar dilution methods. Metallo-β- lactamase- and OXA carbapenemase-producing iso- lates were detected by PCR. Carbapenem resistance and hydrolytic activities were compared according to OXA type and presence of ISAba1. Pulsed-field gel electrophoresis (PFGE) was performed to determine the epidemiologic features. Results: The imipenem non-susceptible rates were variable from 10% to 67%. Among 151 isolates car- rying blaOXA-51-like, 75 isolates carried both blaOXA-51-like and ISAba1, and 25 isolates had both blaOXA-51-like, blaOXA-23-like, and ISAba1. Carbapenem MICs of both blaOXA-51-like and ISAba1-carrying isolates were higher than those with blaOXA-51-like only. Carbapenem MICs of blaOXA-23-like-carrying isolates were higher than those with both blaOXA-51-like and ISAba1. Both blaOXA-51-like and ISAba1-carrying isolates and blaOXA-51-like, blaOXA-23-like, and ISAba1-carrying isolates demonstrated higher hydrolysis activities in oxacillin and carbapenems. Most of the tested isolates were susceptible to tige- cycline, and all of them were susceptible to colistin. Pulsed-field gel electrophoresis suggested that there had been several outbreaks of blaOXA-23-like and blaOXA-51-like-positive strains. Conclusion: Carbapenem non-susceptible Acineto- bacter isolates and OXA carbapenemase-producing isolates were prevalent. Dissemination of blaOXA-har- boring isolates may make it difficult to treat infections due to carbapenem-resistant Acinetobacter spp. Further surveillance studies are required to prevent the spread of carbapenem resistance. (Korean J Clin Microbiol 2010;13:27-33)","PeriodicalId":143093,"journal":{"name":"Korean Journal of Clinical Microbiology","volume":"243 3","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2010-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"114108608","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2010-03-01DOI: 10.5145/KJCM.2010.13.1.19
Hyukmin Lee, E. Koh, Chang Ki Kim, J. Yum, Kyungwon Lee, Y. Chong
Recently a novel plasmid-mediated re-sistant mechanism that conferred high-level resist-ance to aminoglycoside via methylation of 16S rRNA was reported. The aims of this study were to de-termine the prevalence of the 16S rRNA methylase genes and to characterize the coresistance to other antibiotics in Gram-negative bacilli.
{"title":"Molecular and Phenotypic Characteristics of 16S rRNA Methylase-producing Gram-negative Bacilli","authors":"Hyukmin Lee, E. Koh, Chang Ki Kim, J. Yum, Kyungwon Lee, Y. Chong","doi":"10.5145/KJCM.2010.13.1.19","DOIUrl":"https://doi.org/10.5145/KJCM.2010.13.1.19","url":null,"abstract":"Recently a novel plasmid-mediated re-sistant mechanism that conferred high-level resist-ance to aminoglycoside via methylation of 16S rRNA was reported. The aims of this study were to de-termine the prevalence of the 16S rRNA methylase genes and to characterize the coresistance to other antibiotics in Gram-negative bacilli.","PeriodicalId":143093,"journal":{"name":"Korean Journal of Clinical Microbiology","volume":"30 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2010-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125275620","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2010-03-01DOI: 10.5145/KJCM.2010.13.1.40
Chae Lim Jung, Mi Ae Lee, W. Chung
Background: Community-acquired pneumonia (CAP) is a major infectious disease with significant morbidity and mortality worldwide. Streptococcus pneumoniae, Haemophilus influenzae, Mycoplasma pneumoniae, Chlamydophila pneumoniae, Legionella pneumophila, and Bordetella pertussis are common pathogens of CAP; however, the conventional methods used to detect these agents, including culturing, lack sensitivity and are time-consuming. We evaluated a recently developed multiplex PCR assay which can test these agents simultaneously. Methods: One hundred patients with pneumonia and 99 healthy adults were tested using the Seeplex Pneumobacter ACE Detection assay (Seegene, Inc., Seoul, Korea). Culture and urinary antigen tests were also performed. Results: In patients with pneumonia, the positive detection rates of PCR for S. pneumoniae and H. influenzae were 52.0% (52/100) and 30.0% (30/100), respectively, those of M. pneumoniae and L. pneumophila were 2.0% (2/100) and 1.0% (1/100), respectively, and B. pertussis and C. pneumoniae were not detected. In healthy adults, the detection rates of S. pneumoniae and H. influenzae revealed similar results, 53.5% (53/101) and 40.4% (40/101), respectively, and the other four pathogens were not detected. The sensitivity and specificity of PCR for S. pneumoniae in pneumonia patients were 100% (95% confidence interval [CI], 87.9∼100%) and 65.7% (95% CI, 55.2∼76.5%), respectively, according to the urinary antigen test and cultures of the respiratory samples and blood. Conclusion: Differentiating S. pneumoniae and H. influenzae colonization from infection was difficult using the PCR assay. Therefore, the use of this assay is inappropriate for the diagnosis of pneumonia due to these agents, although multiplex PCR assay would be useful for the detection of M. pneumoniae and L. pneumophila. (Korean J Clin Microbiol 2010;13:40-46)
{"title":"Clinical Evaluation of the Multiplex PCR Assay for the Detection of Bacterial Pathogens in Respiratory Specimens from Patients with Pneumonia","authors":"Chae Lim Jung, Mi Ae Lee, W. Chung","doi":"10.5145/KJCM.2010.13.1.40","DOIUrl":"https://doi.org/10.5145/KJCM.2010.13.1.40","url":null,"abstract":"Background: Community-acquired pneumonia (CAP) is a major infectious disease with significant morbidity and mortality worldwide. Streptococcus pneumoniae, Haemophilus influenzae, Mycoplasma pneumoniae, Chlamydophila pneumoniae, Legionella pneumophila, and Bordetella pertussis are common pathogens of CAP; however, the conventional methods used to detect these agents, including culturing, lack sensitivity and are time-consuming. We evaluated a recently developed multiplex PCR assay which can test these agents simultaneously. Methods: One hundred patients with pneumonia and 99 healthy adults were tested using the Seeplex Pneumobacter ACE Detection assay (Seegene, Inc., Seoul, Korea). Culture and urinary antigen tests were also performed. Results: In patients with pneumonia, the positive detection rates of PCR for S. pneumoniae and H. influenzae were 52.0% (52/100) and 30.0% (30/100), respectively, those of M. pneumoniae and L. pneumophila were 2.0% (2/100) and 1.0% (1/100), respectively, and B. pertussis and C. pneumoniae were not detected. In healthy adults, the detection rates of S. pneumoniae and H. influenzae revealed similar results, 53.5% (53/101) and 40.4% (40/101), respectively, and the other four pathogens were not detected. The sensitivity and specificity of PCR for S. pneumoniae in pneumonia patients were 100% (95% confidence interval [CI], 87.9∼100%) and 65.7% (95% CI, 55.2∼76.5%), respectively, according to the urinary antigen test and cultures of the respiratory samples and blood. Conclusion: Differentiating S. pneumoniae and H. influenzae colonization from infection was difficult using the PCR assay. Therefore, the use of this assay is inappropriate for the diagnosis of pneumonia due to these agents, although multiplex PCR assay would be useful for the detection of M. pneumoniae and L. pneumophila. (Korean J Clin Microbiol 2010;13:40-46)","PeriodicalId":143093,"journal":{"name":"Korean Journal of Clinical Microbiology","volume":"14 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2010-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"124023462","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2010-03-01DOI: 10.5145/KJCM.2010.13.1.47
Mi-Ae Jang, Minseok Song, J. Lee, N. Lee
Mycoplasma hominis is a commensal organism in the genitourinary tract. Extragenital infections by M. hominis are rare, and its occurrence is usually limited to immunocompromised patients. Here we report two patients with extragenital infection by M. hominis. The first patient, a woman with angioimmunoblastic T cell lymphoma, underwent autologous peripheral blood stem cell transplantation. The second patient, a woman with endometrial cancer, received laparoscopically-assisted vaginal hysterectomy. They both presented with septic symptoms, including fever, and M. hominis was isolated from pleural effusion and ascitic fluid, respectively. We are reporting these two cases of extragenital infection by M. hominis with a literature review to emphasize that the rapid isolation of M. hominis with early treatment can lead to a better prognosis. (Korean J Clin Microbiol 2010;13:47-50)
{"title":"Two Cases of Extragenital Infection by Mycoplasma hominis","authors":"Mi-Ae Jang, Minseok Song, J. Lee, N. Lee","doi":"10.5145/KJCM.2010.13.1.47","DOIUrl":"https://doi.org/10.5145/KJCM.2010.13.1.47","url":null,"abstract":"Mycoplasma hominis is a commensal organism in the genitourinary tract. Extragenital infections by M. hominis are rare, and its occurrence is usually limited to immunocompromised patients. Here we report two patients with extragenital infection by M. hominis. The first patient, a woman with angioimmunoblastic T cell lymphoma, underwent autologous peripheral blood stem cell transplantation. The second patient, a woman with endometrial cancer, received laparoscopically-assisted vaginal hysterectomy. They both presented with septic symptoms, including fever, and M. hominis was isolated from pleural effusion and ascitic fluid, respectively. We are reporting these two cases of extragenital infection by M. hominis with a literature review to emphasize that the rapid isolation of M. hominis with early treatment can lead to a better prognosis. (Korean J Clin Microbiol 2010;13:47-50)","PeriodicalId":143093,"journal":{"name":"Korean Journal of Clinical Microbiology","volume":"58 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2010-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122804926","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2010-03-01DOI: 10.5145/KJCM.2010.13.1.7
Y. Yoon, H. Sim, J. Kim, D. Park, J. Sohn, B. Chun, M. J. Kim
was independently associated with AKI occurrence in non-fulminant HAV infections (odds ratios=1.094, 95% confidence interval=1.011−1.183). HAV RNA was detected in 57 (86.4%) patients: 53 strains (93.0%) showed genotype IIIA and 4 strains pre- sented genotype IA. All HAV isolates from the AKI patients belonged to the genotype IIIA and shared the identical sequences with those from the non-AKI patients. Conclusion: This study indicates that higher level of C-reactive protein is associated with AKI occurrence in non-fulminant HAV infections. There were no spe- cific nucleotide or amino acid substitutions in the VP1 region associated with AKI occurrence. (Korean J Clin Microbiol 2010;13:7-13)
{"title":"Clinical Characterization of Hepatitis A Infection Complicated with Acute Kidney Injury and Sequence Analysis of the VP1 Region","authors":"Y. Yoon, H. Sim, J. Kim, D. Park, J. Sohn, B. Chun, M. J. Kim","doi":"10.5145/KJCM.2010.13.1.7","DOIUrl":"https://doi.org/10.5145/KJCM.2010.13.1.7","url":null,"abstract":"was independently associated with AKI occurrence in non-fulminant HAV infections (odds ratios=1.094, 95% confidence interval=1.011−1.183). HAV RNA was detected in 57 (86.4%) patients: 53 strains (93.0%) showed genotype IIIA and 4 strains pre- sented genotype IA. All HAV isolates from the AKI patients belonged to the genotype IIIA and shared the identical sequences with those from the non-AKI patients. Conclusion: This study indicates that higher level of C-reactive protein is associated with AKI occurrence in non-fulminant HAV infections. There were no spe- cific nucleotide or amino acid substitutions in the VP1 region associated with AKI occurrence. (Korean J Clin Microbiol 2010;13:7-13)","PeriodicalId":143093,"journal":{"name":"Korean Journal of Clinical Microbiology","volume":"18 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2010-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126481009","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2010-03-01DOI: 10.5145/KJCM.2010.13.1.34
Younhee Park, H. Shin, Chang Ki Kim, K. Roh, J. Yum, D. Yong, S. Jeong, Kyungwon Lee
Department of Laboratory Medicine, Kwandong University College of Medicine, Goyang, Soonchunhyang University College of Medicine, Korea University College of Medicine, Seoul, Department of Clinical Laboratory Science, Dongeui University, Busan, Department of Laboratory Medicine and Research Institute of Bacterial Resistance, Yonsei University College of Medicine, Korean Institute of Tuberculosis, Seoul, Korea
{"title":"Identification of Bacterial and Fungal Isolates by Sequence Analysis of 16S rRNA and Internal Transcribed Spacer","authors":"Younhee Park, H. Shin, Chang Ki Kim, K. Roh, J. Yum, D. Yong, S. Jeong, Kyungwon Lee","doi":"10.5145/KJCM.2010.13.1.34","DOIUrl":"https://doi.org/10.5145/KJCM.2010.13.1.34","url":null,"abstract":"Department of Laboratory Medicine, Kwandong University College of Medicine, Goyang, Soonchunhyang University College of Medicine, Korea University College of Medicine, Seoul, Department of Clinical Laboratory Science, Dongeui University, Busan, Department of Laboratory Medicine and Research Institute of Bacterial Resistance, Yonsei University College of Medicine, Korean Institute of Tuberculosis, Seoul, Korea","PeriodicalId":143093,"journal":{"name":"Korean Journal of Clinical Microbiology","volume":"84 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2010-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"133716556","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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