Pub Date : 2023-06-25DOI: 10.36103/ijas.v51i5.1739
Omar F. H., A. H. Ibrahim
This study aimed to determine antibiotic resistance profiles and the distribution of Integron I, Integron II, and genes among uropathogenic Klebsiella pneumoniae isolated from patients suffering from urinary tract infection. The results revealed that one hundred and thirty-seven (91%) out of 150 clinical specimens were Klebsiella pneumoniae. The isolates (137 isolates) were given a higher percentage of resistance against Piperacillin (94.89%)and Rifampicin (93.43%) and the lowest resistance was with Meropenem (40.87%). According to PCR amplification for 15 isolates showed the proportion of class I (intl1) and class II (intl2) integrons were investigated. What are 100% (n=15) and 45% (n=7) of isolates had intl1 and intl2 genes, respectively. PCR products for (Five)isolates were subjected to direct sequencing, and both strands of PCR products were sequenced with an automatic sequencer. These sequences were analyzed for the presence of variance of these genes and the detection of the differences in the nucleotides.
{"title":"THE PREVALENCE OF INTEGRON CLASS I AND II AMONG MULTI-DRUG RESISTANCE PRODUCING KLEBSIELLA pneumonia","authors":"Omar F. H., A. H. Ibrahim","doi":"10.36103/ijas.v51i5.1739","DOIUrl":"https://doi.org/10.36103/ijas.v51i5.1739","url":null,"abstract":"This study aimed to determine antibiotic resistance profiles and the distribution of Integron I, Integron II, and genes among uropathogenic Klebsiella pneumoniae isolated from patients suffering from urinary tract infection. The results revealed that one hundred and thirty-seven (91%) out of 150 clinical specimens were Klebsiella pneumoniae. The isolates (137 isolates) were given a higher percentage of resistance against Piperacillin (94.89%)and Rifampicin (93.43%) and the lowest resistance was with Meropenem (40.87%). According to PCR amplification for 15 isolates showed the proportion of class I (intl1) and class II (intl2) integrons were investigated. What are 100% (n=15) and 45% (n=7) of isolates had intl1 and intl2 genes, respectively. PCR products for (Five)isolates were subjected to direct sequencing, and both strands of PCR products were sequenced with an automatic sequencer. These sequences were analyzed for the presence of variance of these genes and the detection of the differences in the nucleotides.","PeriodicalId":14562,"journal":{"name":"IRAQI JOURNAL OF AGRICULTURAL SCIENCES","volume":"74 1","pages":""},"PeriodicalIF":0.6,"publicationDate":"2023-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79977163","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-06-25DOI: 10.36103/ijas.v54i3.1771
Amali Abdo Al-Ahmad, Muhammad Batha, Bayan Muzhar
The research was conducted in Tartous governorate on apple trees cv. Golden Delicious grafted on the rootstock “Malus domestica Borkh”, during two seasons 2019-2020 to study the impact of manual and chemical thinning using foliar boron /zinc spray separately or in combination on some morphological and physiological indicators. The results showed a significant increase in leaf area and its content of total chlorophyll and carotenoids in the second season (low fruit load) compared to the first season (heavy load) due to a rise in vegetative growth and a reduction in fruiting. During the heavy load season, a balance between vegetative and fruiting growth was noted when the manual and chemical thinning applied with no significant differences was found between the two seasons in leaf area, total chlorophyll and carotenoids content. On the other hand, the application of manual thinning, chemical thinning and foliar spraying with boron and zinc separately or together pointed out an increase in the branches content of carbohydrates and nitrogen and C/N ratio in the low load season compared to the control. Consequently, fruit thinning was more effective to obtain a balance between the seasons in term of leaf area, its pigments content, and C/N ratio in branches, which in turn mitigate the alternate bearing phenomenon.
{"title":"IMPACT OF THINNING AND FOLIAR-BORON-ZINC SPRAY ON SOME MORPHOLOGICAL AND PHYSIOLOGICAL INDICATORS IN APPLE TREE (MALUS DOMESTICA CV. GOLDEN DELICIOUS)-\"TARTOUS GOVERNORATE\"","authors":"Amali Abdo Al-Ahmad, Muhammad Batha, Bayan Muzhar","doi":"10.36103/ijas.v54i3.1771","DOIUrl":"https://doi.org/10.36103/ijas.v54i3.1771","url":null,"abstract":"The research was conducted in Tartous governorate on apple trees cv. Golden Delicious grafted on the rootstock “Malus domestica Borkh”, during two seasons 2019-2020 to study the impact of manual and chemical thinning using foliar boron /zinc spray separately or in combination on some morphological and physiological indicators. The results showed a significant increase in leaf area and its content of total chlorophyll and carotenoids in the second season (low fruit load) compared to the first season (heavy load) due to a rise in vegetative growth and a reduction in fruiting. During the heavy load season, a balance between vegetative and fruiting growth was noted when the manual and chemical thinning applied with no significant differences was found between the two seasons in leaf area, total chlorophyll and carotenoids content. On the other hand, the application of manual thinning, chemical thinning and foliar spraying with boron and zinc separately or together pointed out an increase in the branches content of carbohydrates and nitrogen and C/N ratio in the low load season compared to the control. Consequently, fruit thinning was more effective to obtain a balance between the seasons in term of leaf area, its pigments content, and C/N ratio in branches, which in turn mitigate the alternate bearing phenomenon. ","PeriodicalId":14562,"journal":{"name":"IRAQI JOURNAL OF AGRICULTURAL SCIENCES","volume":"6 1","pages":""},"PeriodicalIF":0.6,"publicationDate":"2023-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87570689","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-06-25DOI: 10.36103/ijas.v54i3.1749
M. S. K. M. S. S. Researcher, P. Dept., Coll. Sci Biot.
The purpose of this research was to find the effect of alcoholic extract of the lion's mane mushroom (Hericium erinaceus) against certain clinical pathological isolates isolated from Iraqi patients suffering from various diseases such as gingivitis, urinary tract infection, diarrhea and severe vomiting, and also a biopsy was taken from the stomach of people suffering from ulcers. Out of 150 males and females sample 107 samples were isolated and identified by chemical detection and VITEK 2 technique, and by PCR for Halicobacter pylori as follows: Staph aureus(56),Salmonella typhi(6),Pseudomonas aeruginosa(10),Escherichia coli(15),Halicobacter pylori(10),Klebsiella pneumonia(10),Candida albicans(10) samples. lion’s mane mushroom powder was extracted with 80% ethyl alcohol, and active components were investigated by chemical reagents and Gc-mass. The chemical detection of Alcoholic extract revealed the existence of flavonoids, alkaloids, phenols, terpenoids, glycosides, and polysaccharides. The efficacy of the extract was tested using 4 concentrations (400, 200, 100, and 50 mg/ml) on bacterial and yeast isolate (C.albicans).The diameter of the inhibitory zone increased with increasing extract concentrations, according to the findings. Zone of inhibition for all isolates started at 100mg/ml except C.albicans(50mg/ml)and E.coli (200mg/ml)
{"title":"ANTIBATERIAL ACTIVITY OF (HERICIUM ERINACEUS) EXTRACT ON SOME CLINICAL PATHOGENIC ISOLATES","authors":"M. S. K. M. S. S. Researcher, P. Dept., Coll. Sci Biot.","doi":"10.36103/ijas.v54i3.1749","DOIUrl":"https://doi.org/10.36103/ijas.v54i3.1749","url":null,"abstract":"The purpose of this research was to find the effect of alcoholic extract of the lion's mane mushroom (Hericium erinaceus) against certain clinical pathological isolates isolated from Iraqi patients suffering from various diseases such as gingivitis, urinary tract infection, diarrhea and severe vomiting, and also a biopsy was taken from the stomach of people suffering from ulcers. Out of 150 males and females sample 107 samples were isolated and identified by chemical detection and VITEK 2 technique, and by PCR for Halicobacter pylori as follows: Staph aureus(56),Salmonella typhi(6),Pseudomonas aeruginosa(10),Escherichia coli(15),Halicobacter pylori(10),Klebsiella pneumonia(10),Candida albicans(10) samples. lion’s mane mushroom powder was extracted with 80% ethyl alcohol, and active components were investigated by chemical reagents and Gc-mass. The chemical detection of Alcoholic extract revealed the existence of flavonoids, alkaloids, phenols, terpenoids, glycosides, and polysaccharides. The efficacy of the extract was tested using 4 concentrations (400, 200, 100, and 50 mg/ml) on bacterial and yeast isolate (C.albicans).The diameter of the inhibitory zone increased with increasing extract concentrations, according to the findings. Zone of inhibition for all isolates started at 100mg/ml except C.albicans(50mg/ml)and E.coli (200mg/ml)","PeriodicalId":14562,"journal":{"name":"IRAQI JOURNAL OF AGRICULTURAL SCIENCES","volume":"52 1","pages":""},"PeriodicalIF":0.6,"publicationDate":"2023-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73322603","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-06-25DOI: 10.36103/ijas.v54i3.1755
S. Jaffer Ramadhan, K. Khadim Khudair, B. Najim Al-Okaily
This study was aimed to explore the role of gallic acid (GA) in ameliorating in reducing adverse effects of cadmium chloride (CdCl2) on antioxidant status and lipid profile in adult male rats. Twenty-eight (28) adult male rats were divided randomly into four equal groups; they were daily handled for 30 days, as follows: control group (C), received tap water only. (G1), received 100ppm of CdCl2 in drinking tap water, animals in proceeding groups were given in addition to CdCl2 in drinking water the following: intraperitoneal injection of GA 100 mg/kg. daily (G2 group) and the combination of GA and CdCl2 were given to rats in group (G3) in the same pattern. At the end of the experiment, fasting blood samples were collected and serum was isolated for measuring of antioxidant status and lipid profile. The results showed that administration CdCl2 (G1 group) caused a case of dyslipidemia illustrated by significant elevation in serum cholesterol concentration in lipoprotein low density lipoprotein-cholesterol (LDL-C) and very low-density lipoprotein-cholesterol (VLDL-C), total cholesterol (TC), triglyceride (TAG) and non-HDL-C accompanied with significant decrease in cholesterol of high density lipoprotein (HDL-C) concentrations. The results also revealed a significant elevation in lipid indices including, coronary risk index (CRI), and cardiovascular risk index (CVRI) in CdCl2 exposed rats. While significant elevation in malondialdehyde (MDA) and reduction in (GSH) concentrations observed in the same group comparing to gallic acid and control group, indicating a case of oxidative stress. The current results also recorded that intraperitoneal injection of GA against CdCl2 caused amelioration of all previously estimated parameters.
{"title":"EFFECT OF GALLIC ACID ON LIPID PROFILE AND ANTIOXIDANT STATUS IN CADMIUM CHLORIDE TREATED RATS","authors":"S. Jaffer Ramadhan, K. Khadim Khudair, B. Najim Al-Okaily","doi":"10.36103/ijas.v54i3.1755","DOIUrl":"https://doi.org/10.36103/ijas.v54i3.1755","url":null,"abstract":"This study was aimed to explore the role of gallic acid (GA) in ameliorating in reducing adverse effects of cadmium chloride (CdCl2) on antioxidant status and lipid profile in adult male rats. Twenty-eight (28) adult male rats were divided randomly into four equal groups; they were daily handled for 30 days, as follows: control group (C), received tap water only. (G1), received 100ppm of CdCl2 in drinking tap water, animals in proceeding groups were given in addition to CdCl2 in drinking water the following: intraperitoneal injection of GA 100 mg/kg. daily (G2 group) and the combination of GA and CdCl2 were given to rats in group (G3) in the same pattern. At the end of the experiment, fasting blood samples were collected and serum was isolated for measuring of antioxidant status and lipid profile. The results showed that administration CdCl2 (G1 group) caused a case of dyslipidemia illustrated by significant elevation in serum cholesterol concentration in lipoprotein low density lipoprotein-cholesterol (LDL-C) and very low-density lipoprotein-cholesterol (VLDL-C), total cholesterol (TC), triglyceride (TAG) and non-HDL-C accompanied with significant decrease in cholesterol of high density lipoprotein (HDL-C) concentrations. The results also revealed a significant elevation in lipid indices including, coronary risk index (CRI), and cardiovascular risk index (CVRI) in CdCl2 exposed rats. While significant elevation in malondialdehyde (MDA) and reduction in (GSH) concentrations observed in the same group comparing to gallic acid and control group, indicating a case of oxidative stress. The current results also recorded that intraperitoneal injection of GA against CdCl2 caused amelioration of all previously estimated parameters.","PeriodicalId":14562,"journal":{"name":"IRAQI JOURNAL OF AGRICULTURAL SCIENCES","volume":"56 1","pages":""},"PeriodicalIF":0.6,"publicationDate":"2023-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91387104","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-06-25DOI: 10.36103/ijas.v54i3.1770
H. Y. Khalaf
The current study was aimed to determined the main causes of ringworm in cows and antifungal and synergistic effects of ZnO nanoparticles. For this purpose 50 skin scrapes were collected from cows infected with ringworm, culture media, staining and genetic methods used for diagnosis. MIC and MFC for antifungal and ZnO were determined. The result showed that Trichophyton spp was isolated in rate of 76%. The isolation rate of T.verrucosum, T. mentagrophytes and T. rubrum were 68.4%, 21.0% and 10.5% respectively. MIC of Nystatin, fluocytosin, ZnO, Nystatin+ ZnO and Fluocytosin + ZnO were 200,150,200,150 and 100 μg/ml respectively. in conclusion, that T.verrucosum is main caused of Ringworm and ZnO has antifungal and synergistic effects.
{"title":"ANTIFUNGAL AND SYNERGISTIC EFFECTS OF ZNO NANOPARTICLES AGAINST T.VERRUCOSUM CAUSED RINGWORM IN COWS","authors":"H. Y. Khalaf","doi":"10.36103/ijas.v54i3.1770","DOIUrl":"https://doi.org/10.36103/ijas.v54i3.1770","url":null,"abstract":"The current study was aimed to determined the main causes of ringworm in cows and antifungal and synergistic effects of ZnO nanoparticles. For this purpose 50 skin scrapes were collected from cows infected with ringworm, culture media, staining and genetic methods used for diagnosis. MIC and MFC for antifungal and ZnO were determined. The result showed that Trichophyton spp was isolated in rate of 76%. The isolation rate of T.verrucosum, T. mentagrophytes and T. rubrum were 68.4%, 21.0% and 10.5% respectively. MIC of Nystatin, fluocytosin, ZnO, Nystatin+ ZnO and Fluocytosin + ZnO were 200,150,200,150 and 100 μg/ml respectively. in conclusion, that T.verrucosum is main caused of Ringworm and ZnO has antifungal and synergistic effects.","PeriodicalId":14562,"journal":{"name":"IRAQI JOURNAL OF AGRICULTURAL SCIENCES","volume":"19 1","pages":""},"PeriodicalIF":0.6,"publicationDate":"2023-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79413827","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-06-25DOI: 10.36103/ijas.v54i3.1754
K. A. Hadi, S. D. S. Al-douri, D. H. Hadree
The present study was carried out to investigate the effect of folic acid (FA) on the hematological picture of female rabbits treated with methotrexate MTX. A total of twenty female rabbits were used in this study. They were at age 4-5 months. Their body weight ranged between 1-1,200 Kgm. All animals were kept under normal condition. its divided in to four groups and each group consist of five animals as follows ( Control group :5 rabbits were received distilled water, Folic acid group : 5 rabbits were received folic acid at 0.07mg/kg body weight daily, Methotrexate group : 5 rabbits were received methtrexate (0.03 mg/kg body weight ) three times a week , folic acid with Methotrexate group : 5 rabbits were received folic acid (0.07 mg/kg body weight) and methotrexate (0.03mg/kg body weight) three times a week. The drugs were given by intubation. The experiment was last for 9 weeks . Blood sample were collected after end of the experiment to study the following hematological parameters: RBCs count, Hb, PCV, RBCs indices (MCV, MCH, MCHC). The group treated with folic acid showed a significant increase P≤0.05 in RBCs count, Hb conc. and PCV% as compared with all other groups. The results of MTX group reveal a high significant decrease in their RBC count, Hb conc., PCV%. At the same time there is a significant increase in MCV, MCH and MCHC indices. The group of animals received FA with MTX showed a good prognosis with health improvement characterized by high significant changes in all studied parameters to return back to their normal values. It was concluded that folic acid is very important for erythropoiesis. MTX treatment induce megaloblastic anemia resulted from inhibition of DNA synthesis in RBCs mainly by folate deficiency. FA administration with MTX correct these changes and the animals return to normal conditions. More work is needed to study the effects of these drugs on other systems in the body.
{"title":"EFFECT OF FOLIC ACID ON SOME PHYSIOLOGICAL PARAMETERS IN FEMALE RABBITS TREATED WITH METHOTREXATE","authors":"K. A. Hadi, S. D. S. Al-douri, D. H. Hadree","doi":"10.36103/ijas.v54i3.1754","DOIUrl":"https://doi.org/10.36103/ijas.v54i3.1754","url":null,"abstract":"The present study was carried out to investigate the effect of folic acid (FA) on the hematological picture of female rabbits treated with methotrexate MTX. A total of twenty female rabbits were used in this study. They were at age 4-5 months. Their body weight ranged between 1-1,200 Kgm. All animals were kept under normal condition. its divided in to four groups and each group consist of five animals as follows ( Control group :5 rabbits were received distilled water, Folic acid group : 5 rabbits were received folic acid at 0.07mg/kg body weight daily, Methotrexate group : 5 rabbits were received methtrexate (0.03 mg/kg body weight ) three times a week , folic acid with Methotrexate group : 5 rabbits were received folic acid (0.07 mg/kg body weight) and methotrexate (0.03mg/kg body weight) three times a week. The drugs were given by intubation. The experiment was last for 9 weeks . Blood sample were collected after end of the experiment to study the following hematological parameters: RBCs count, Hb, PCV, RBCs indices (MCV, MCH, MCHC). The group treated with folic acid showed a significant increase P≤0.05 in RBCs count, Hb conc. and PCV% as compared with all other groups. The results of MTX group reveal a high significant decrease in their RBC count, Hb conc., PCV%. At the same time there is a significant increase in MCV, MCH and MCHC indices. The group of animals received FA with MTX showed a good prognosis with health improvement characterized by high significant changes in all studied parameters to return back to their normal values. It was concluded that folic acid is very important for erythropoiesis. MTX treatment induce megaloblastic anemia resulted from inhibition of DNA synthesis in RBCs mainly by folate deficiency. FA administration with MTX correct these changes and the animals return to normal conditions. More work is needed to study the effects of these drugs on other systems in the body.","PeriodicalId":14562,"journal":{"name":"IRAQI JOURNAL OF AGRICULTURAL SCIENCES","volume":"6 1","pages":""},"PeriodicalIF":0.6,"publicationDate":"2023-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79519886","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-06-25DOI: 10.36103/ijas.v54i3.1758
K. S. Rajab, A. O. E. Lecturer, P. Dept., Soil Water, Coll, Agric, Engin, Sci., Salaheddin
This study was carried out in Erbil governorate, Iraq during 2020 to estimate the regression relations between the ionic strength of 354 water samples and their electrical conductivity then influence of correcting ion pairs and activity on this relation. Results indicated that electrical conductivity regards as an accurate measure of ionic strength for irrigation water. Correcting ion pairs plus activity had a great effect on the regression values between ionic strength values and electrical conductivity of the studied water samples. Highly significant correlation coefficient was recorded between ionic strength and electrical conductivity before correction and after correcting ion pairs and ion pairs plus activity with the correlation coefficient values of (r= 0.99**, 0.93** and 0.97**) respectively. Correcting ion pairs and ion pairs plus activity caused a decrease in the slope of the regression from 0.0157 to 0.0104 and 0.0047 respectively. Neglecting intercept values from the regression line caused an increase in the slope of regression relation to 0.0112 and 0.0058 for correcting ion pairs and ion pairs plus activity respectively.
{"title":"ROLE OF ION PAIRS AND ACTIVITY IN ESTIMATION OF IONIC STRENGTH FROM ELECTRICAL CONDUCTIVITY OF IRRIGATION WATER","authors":"K. S. Rajab, A. O. E. Lecturer, P. Dept., Soil Water, Coll, Agric, Engin, Sci., Salaheddin","doi":"10.36103/ijas.v54i3.1758","DOIUrl":"https://doi.org/10.36103/ijas.v54i3.1758","url":null,"abstract":"This study was carried out in Erbil governorate, Iraq during 2020 to estimate the regression relations between the ionic strength of 354 water samples and their electrical conductivity then influence of correcting ion pairs and activity on this relation. Results indicated that electrical conductivity regards as an accurate measure of ionic strength for irrigation water. Correcting ion pairs plus activity had a great effect on the regression values between ionic strength values and electrical conductivity of the studied water samples. Highly significant correlation coefficient was recorded between ionic strength and electrical conductivity before correction and after correcting ion pairs and ion pairs plus activity with the correlation coefficient values of (r= 0.99**, 0.93** and 0.97**) respectively. Correcting ion pairs and ion pairs plus activity caused a decrease in the slope of the regression from 0.0157 to 0.0104 and 0.0047 respectively. Neglecting intercept values from the regression line caused an increase in the slope of regression relation to 0.0112 and 0.0058 for correcting ion pairs and ion pairs plus activity respectively.","PeriodicalId":14562,"journal":{"name":"IRAQI JOURNAL OF AGRICULTURAL SCIENCES","volume":"11 1","pages":""},"PeriodicalIF":0.6,"publicationDate":"2023-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87412311","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-06-25DOI: 10.36103/ijas.v54i3.1750
Teeba G. Albadri, Mouruj A. Alaubydi
This study was aimed to conducted as a challenge test for R. mucosa ability to reduces atopic dermatitis induced in the skin of experimental animals and improve the fact that this kind of microorganism play important role as skin microflora to reduce some type of skin diseases, additionally to find the suitable pharmaceutical formula can be maintaining and stimulating bacterial isolate to produce a pigment. A probiotic application as a suitable cream formulas were prepared containing local R. mucosa biomass, Standard R. mucosa biomass and partial purified pigment to treat three groups of albino mice induced with atopic dermatitis. The main physical characteristics of the prepared cream formula are homogeneous with odorless, smooth texture, and clarity of its light yellowish color. Some immunological test including (CBC, lymphocyte, neutrophil, platelets), total serum IgE and histopathological analysis were also done. The in vivo result improve the effectivity of all prepared formulas particularly local R. mucosa biomass formula in reducing induced AD in mice either externally or internally. The findings suggested the safety of topical R. mucosa therapy of lab. animals induced with AD.
{"title":"DETERMINATION SOME IMMUNOLOGICAL AND HISTOPATHOLOGICAL PARAMETERS RELATED WITH ROSEOMONAS MUCOSA AND ITS PIGMENT IN PHARMACEUTICAL FORMULAS.","authors":"Teeba G. Albadri, Mouruj A. Alaubydi","doi":"10.36103/ijas.v54i3.1750","DOIUrl":"https://doi.org/10.36103/ijas.v54i3.1750","url":null,"abstract":"This study was aimed to conducted as a challenge test for R. mucosa ability to reduces atopic dermatitis induced in the skin of experimental animals and improve the fact that this kind of microorganism play important role as skin microflora to reduce some type of skin diseases, additionally to find the suitable pharmaceutical formula can be maintaining and stimulating bacterial isolate to produce a pigment. A probiotic application as a suitable cream formulas were prepared containing local R. mucosa biomass, Standard R. mucosa biomass and partial purified pigment to treat three groups of albino mice induced with atopic dermatitis. The main physical characteristics of the prepared cream formula are homogeneous with odorless, smooth texture, and clarity of its light yellowish color. Some immunological test including (CBC, lymphocyte, neutrophil, platelets), total serum IgE and histopathological analysis were also done. The in vivo result improve the effectivity of all prepared formulas particularly local R. mucosa biomass formula in reducing induced AD in mice either externally or internally. The findings suggested the safety of topical R. mucosa therapy of lab. animals induced with AD.","PeriodicalId":14562,"journal":{"name":"IRAQI JOURNAL OF AGRICULTURAL SCIENCES","volume":"134 1","pages":""},"PeriodicalIF":0.6,"publicationDate":"2023-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73072044","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-06-25DOI: 10.36103/ijas.v54i3.1743
Z. A. Abdulkareem, A. Al-kazaz, Researcher Prof, Dept Biotechn, Coll, Scie
The objective of this study was to evaluate the association and effect of CRISP2 variants on the risk of asthenozoospermia, a male infertility condition marked by absent or diminished sperm motility. There are numerous reasons why individuals develop asthenozoospermia. Therefore, it is crucial to understand the molecular mechanisms underlying this condition of infertility. Furthermore, seminal plasma, a rich source of sperm quality-related biomarkers, transports the many spermatozoa pools that make up human ejaculate down the epididymis. These spermatozoa vary in size, shape, and motility. The morphology and mobility of male ejaculated spermatozoa are affected by a number of genes, including CRISP2. In seminal samples from 120 Iraqi infertile male patients and 40 healthy males who were matched for age, gender, and ethnicity as a control group, the connection of the CRISP2 gene single nucleotide polymorphisms L56V, M176I, and C196R with infertility was investigated. According to statistical analysis of the genotype distribution of these three nsSNPs of the CRISP2 gene in patients with the asthenozoospermia subgroup and the control group, there weren't detectable differences in genotype distribution between AS, OAS, OTA, and fertile men in the Iraqi research sample. Based on allele frequencies, C, T, and G were determined to be protective alleles, with OR values of 0.74, 0.64, and 2.0, respectively.
{"title":"GENETIC POLYMORPHISMS OF CRISP2 GENE IN ASSOCIATION WITH INFERTILITY IN IRAQI PATIENTS","authors":"Z. A. Abdulkareem, A. Al-kazaz, Researcher Prof, Dept Biotechn, Coll, Scie","doi":"10.36103/ijas.v54i3.1743","DOIUrl":"https://doi.org/10.36103/ijas.v54i3.1743","url":null,"abstract":"The objective of this study was to evaluate the association and effect of CRISP2 variants on the risk of asthenozoospermia, a male infertility condition marked by absent or diminished sperm motility. There are numerous reasons why individuals develop asthenozoospermia. Therefore, it is crucial to understand the molecular mechanisms underlying this condition of infertility. Furthermore, seminal plasma, a rich source of sperm quality-related biomarkers, transports the many spermatozoa pools that make up human ejaculate down the epididymis. These spermatozoa vary in size, shape, and motility. The morphology and mobility of male ejaculated spermatozoa are affected by a number of genes, including CRISP2. In seminal samples from 120 Iraqi infertile male patients and 40 healthy males who were matched for age, gender, and ethnicity as a control group, the connection of the CRISP2 gene single nucleotide polymorphisms L56V, M176I, and C196R with infertility was investigated. According to statistical analysis of the genotype distribution of these three nsSNPs of the CRISP2 gene in patients with the asthenozoospermia subgroup and the control group, there weren't detectable differences in genotype distribution between AS, OAS, OTA, and fertile men in the Iraqi research sample. Based on allele frequencies, C, T, and G were determined to be protective alleles, with OR values of 0.74, 0.64, and 2.0, respectively.","PeriodicalId":14562,"journal":{"name":"IRAQI JOURNAL OF AGRICULTURAL SCIENCES","volume":"5 1","pages":""},"PeriodicalIF":0.6,"publicationDate":"2023-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72978619","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-06-25DOI: 10.36103/ijas.v54i3.1762
Shawin A. Khdir, N. S. Ahmad, E. O. Hama-Ali, Sh. M. Abdullah
The objectives of this study were to estimate the performance of the common bean (Phaseolus vulgaris L.) genotypes under water-stress conditions, and their genetic diversity. White bean surpassed the others for relative water content, root/shoot ratio and leaf area under water-stress condition. Scatter plot indicates a strong association of yield with pod numbers plant-1, branch number and harvest index. A total of 69 polymorphic were obtained, applying 26 SSR primers on 14 genotypes. Major allele frequency was 0.601, and the average value of PIC was 0.407. The highest value of gene diversity (0.745) and PIC (0.704) were recorded for BMd-23 marker. Molecular variance among population indicated 25%, while 47% was realized within populations. Structure analysis divided the common bean genotypes into three groups (DeltaK value =3). Chity and Boschbohnen were identified to have a mixed ancestor while all the others were pure at their populations. A dendrogram and PCoA analyses are accordingly indicated three groups of the genotypes based on SSR marker data. STRUCTURE, UPGMA and PCoA analysis revealed the presence of two separated gene pools of Andean and Mesoamerican common beans, with a high level of genetic differentiation (FST value=0.250). Both phenotypic and molecular genetic outcomes here would accelerate future improvement programs.
{"title":"GENETIC DIVERSITY AND POPULATION STRUCTURE OF COMMON BEAN GENOTYPES USING MORPHOLOGICAL TRAITS AND SSR","authors":"Shawin A. Khdir, N. S. Ahmad, E. O. Hama-Ali, Sh. M. Abdullah","doi":"10.36103/ijas.v54i3.1762","DOIUrl":"https://doi.org/10.36103/ijas.v54i3.1762","url":null,"abstract":"The objectives of this study were to estimate the performance of the common bean (Phaseolus vulgaris L.) genotypes under water-stress conditions, and their genetic diversity. White bean surpassed the others for relative water content, root/shoot ratio and leaf area under water-stress condition. Scatter plot indicates a strong association of yield with pod numbers plant-1, branch number and harvest index. A total of 69 polymorphic were obtained, applying 26 SSR primers on 14 genotypes. Major allele frequency was 0.601, and the average value of PIC was 0.407. The highest value of gene diversity (0.745) and PIC (0.704) were recorded for BMd-23 marker. Molecular variance among population indicated 25%, while 47% was realized within populations. Structure analysis divided the common bean genotypes into three groups (DeltaK value =3). Chity and Boschbohnen were identified to have a mixed ancestor while all the others were pure at their populations. A dendrogram and PCoA analyses are accordingly indicated three groups of the genotypes based on SSR marker data. STRUCTURE, UPGMA and PCoA analysis revealed the presence of two separated gene pools of Andean and Mesoamerican common beans, with a high level of genetic differentiation (FST value=0.250). Both phenotypic and molecular genetic outcomes here would accelerate future improvement programs.","PeriodicalId":14562,"journal":{"name":"IRAQI JOURNAL OF AGRICULTURAL SCIENCES","volume":"36 1","pages":""},"PeriodicalIF":0.6,"publicationDate":"2023-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84322290","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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