The authors found interesting results on acetylenic compounds by microbial and animal tests, which are described herein.It is known that same acetylenic compounds such as dicarbamoylacetylen (Collocidin: Lenamycin) and Capillin have antimicrobial activity. During the course of the synthetic study in this laboratory to find those acetylenic compounds with antimicrobial activity, we came to new compounds, halophenyl-γ-iodopropargyl ethers which have very potent activity on fungi and some other microbes. Among these compounds, 2, 4, 5-trichlorophenyl-γ-iodopropargyl ether had each minimal inhibitory concentration of 0.25γ/ml, 0.25γ/ml, 0.78γ/ml against Trichophyton asteroides, T. interdigitale, T. rubrum and also showed rather high activity for other fungi, Cryptococcus, Staphylococcus, Streptococcus, Bacillus subtilis, Mycobacterium in dilution assay.The high activity of the compound for Trichophyton in vitro was also proved by the curative effects ton infectious animal tests.(guinea-pig)
{"title":"Studies on the Acetylenic Compounds","authors":"B. Nomiya, K. Kagino","doi":"10.3412/JSB.18.28","DOIUrl":"https://doi.org/10.3412/JSB.18.28","url":null,"abstract":"The authors found interesting results on acetylenic compounds by microbial and animal tests, which are described herein.It is known that same acetylenic compounds such as dicarbamoylacetylen (Collocidin: Lenamycin) and Capillin have antimicrobial activity. During the course of the synthetic study in this laboratory to find those acetylenic compounds with antimicrobial activity, we came to new compounds, halophenyl-γ-iodopropargyl ethers which have very potent activity on fungi and some other microbes. Among these compounds, 2, 4, 5-trichlorophenyl-γ-iodopropargyl ether had each minimal inhibitory concentration of 0.25γ/ml, 0.25γ/ml, 0.78γ/ml against Trichophyton asteroides, T. interdigitale, T. rubrum and also showed rather high activity for other fungi, Cryptococcus, Staphylococcus, Streptococcus, Bacillus subtilis, Mycobacterium in dilution assay.The high activity of the compound for Trichophyton in vitro was also proved by the curative effects ton infectious animal tests.(guinea-pig)","PeriodicalId":14812,"journal":{"name":"Japanese journal of bacteriology","volume":"90 1","pages":"28-37"},"PeriodicalIF":0.0,"publicationDate":"1963-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80442610","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Two protein fractions II and III, obtained from human strain Aoyama-B tuberculin were tested on their sensitizing-activities comparing with DT and PPD-s.Four groups of guinea pigs were sensitized respectively by intramuscular injectionof fractions II, III, DT, or PPD-s mixed with Bayol F and Arlacel A. Thereafter animals were tested periodically on their skin reactivities with the intradermal injection of the same dose of fractions II, and III, DT, and PPD-s.Results were as follows:1) Animals were well sensitized in all groups and developed the maximal reactivity at 6th week after sensitization. The sensitizing-activity to induce the skin reaction to 5 mcg PPD-s in g-uinea pigs fell into the following sequence; DT, PPD-s, fraction II and fraction III.2) The animals sensitized with fraction II alone developed strong skin reaction to homologous antigen, fraction II.3) In the animals sensitized with DT or fraction II, the maximal reaction was observed at 24hrs. after intradermal injection of homologous antigen, respectively, but it was 8hrs. after injection with other antigens.
从人青山b结核菌中分离得到2个蛋白片段II和III，比较其对DT和PPD-s的致敏活性。四组豚鼠分别通过肌肉注射与巴约尔F和阿拉赛尔a混合的组分II、III、DT或PPD-s致敏，然后定期皮内注射相同剂量的组分II、III、DT和PPD-s，检测动物的皮肤反应性。结果表明:1)各组动物致敏效果良好，致敏后第6周反应活性达到最大。5 mcg PPD-s诱导豚鼠皮肤反应的致敏活性依次为:DT、PPD-s、部分II和部分III.2)单独部分II致敏动物对同种抗原、部分II产生强烈的皮肤反应。3)DT或部分II致敏动物在24h时出现最大反应。分别皮内注射同源抗原后，均为8h。注射其他抗原后。
{"title":"Fractionation of human strain Aoyama-B tuberculin by the use of Zone electrophoresis","authors":"T. Kataoka","doi":"10.3412/jsb.18.109","DOIUrl":"https://doi.org/10.3412/jsb.18.109","url":null,"abstract":"Two protein fractions II and III, obtained from human strain Aoyama-B tuberculin were tested on their sensitizing-activities comparing with DT and PPD-s.Four groups of guinea pigs were sensitized respectively by intramuscular injectionof fractions II, III, DT, or PPD-s mixed with Bayol F and Arlacel A. Thereafter animals were tested periodically on their skin reactivities with the intradermal injection of the same dose of fractions II, and III, DT, and PPD-s.Results were as follows:1) Animals were well sensitized in all groups and developed the maximal reactivity at 6th week after sensitization. The sensitizing-activity to induce the skin reaction to 5 mcg PPD-s in g-uinea pigs fell into the following sequence; DT, PPD-s, fraction II and fraction III.2) The animals sensitized with fraction II alone developed strong skin reaction to homologous antigen, fraction II.3) In the animals sensitized with DT or fraction II, the maximal reaction was observed at 24hrs. after intradermal injection of homologous antigen, respectively, but it was 8hrs. after injection with other antigens.","PeriodicalId":14812,"journal":{"name":"Japanese journal of bacteriology","volume":"11 1","pages":"109-114"},"PeriodicalIF":0.0,"publicationDate":"1963-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74147621","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Using international typing phages 80 and 81, erythromycin-resistance was transduced from naturally resistant strains of Staph. aureus to erythromycin-sensitive strains of both Staph. aureus and Staph. epidermidis.160 strains of Staph. aureus which were highly sensitive to erythromycin were tested for the competence to serve as the recipients of erythromycin-resistance. All cases of transduction experiments revealed that about 10-17% of the tested strains were competent recipient, and all the transductants tested were resistant to 100mcg/ml of erythromycin. In 49 strains of Staph. epidermidis three or four strains were competent recipient, although the degree of resistance of the transductants were rather low compared with that of the appropriate donor.Some of the transductants transduced by the donor which was also highly resistant to oleandomycin received also the oleandomycin-resistance, while in the other transductants oleandomycin-resistance failed to be transmitted. Furthermore, joint transduction of penicillin- or streptomycin-resistance was observed in some of the transductants, but tetracycline-resistance of the donor was not jointly transduced.No change in the biological functions was detected in all of the transductants, and many of the transductants of Staph. aureus showed the same phage types as the original strains.
{"title":"Transduction of Erythromycin-resistance in Staphylococcus","authors":"C. Niwa","doi":"10.3412/JSB.18.21","DOIUrl":"https://doi.org/10.3412/JSB.18.21","url":null,"abstract":"Using international typing phages 80 and 81, erythromycin-resistance was transduced from naturally resistant strains of Staph. aureus to erythromycin-sensitive strains of both Staph. aureus and Staph. epidermidis.160 strains of Staph. aureus which were highly sensitive to erythromycin were tested for the competence to serve as the recipients of erythromycin-resistance. All cases of transduction experiments revealed that about 10-17% of the tested strains were competent recipient, and all the transductants tested were resistant to 100mcg/ml of erythromycin. In 49 strains of Staph. epidermidis three or four strains were competent recipient, although the degree of resistance of the transductants were rather low compared with that of the appropriate donor.Some of the transductants transduced by the donor which was also highly resistant to oleandomycin received also the oleandomycin-resistance, while in the other transductants oleandomycin-resistance failed to be transmitted. Furthermore, joint transduction of penicillin- or streptomycin-resistance was observed in some of the transductants, but tetracycline-resistance of the donor was not jointly transduced.No change in the biological functions was detected in all of the transductants, and many of the transductants of Staph. aureus showed the same phage types as the original strains.","PeriodicalId":14812,"journal":{"name":"Japanese journal of bacteriology","volume":"111 3S 1","pages":"21-27"},"PeriodicalIF":0.0,"publicationDate":"1963-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76165143","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
An 0 agglutinable mutant was isolated from the ultraviolet-irradiated culture of Vibrio parahemolyticus. A-55 (Type 13). This mutant retained the flagellar H and somatic O antigen of its parent strain but lacked in a specific antigenic component which was considered as the K (Kapsel) antigen. The behavior against heat and HC1 of the K antigen, as revealed by agglutination and absorption tests and by antibody formation in the rabbit, resembled that of the B antigen found in certain ofE. coli.The O antigen of the mutant, naked of the K antigen, was destroyed by a treatment with HC1 resembling 5 antigen in certain of theSalmonella.
{"title":"Serological Properties of K-antigen of Vibrio parahemolyticus A-55 (Type 13)","authors":"G. Omori, M. Iwao","doi":"10.3412/JSB.18.379","DOIUrl":"https://doi.org/10.3412/JSB.18.379","url":null,"abstract":"An 0 agglutinable mutant was isolated from the ultraviolet-irradiated culture of Vibrio parahemolyticus. A-55 (Type 13). This mutant retained the flagellar H and somatic O antigen of its parent strain but lacked in a specific antigenic component which was considered as the K (Kapsel) antigen. The behavior against heat and HC1 of the K antigen, as revealed by agglutination and absorption tests and by antibody formation in the rabbit, resembled that of the B antigen found in certain ofE. coli.The O antigen of the mutant, naked of the K antigen, was destroyed by a treatment with HC1 resembling 5 antigen in certain of theSalmonella.","PeriodicalId":14812,"journal":{"name":"Japanese journal of bacteriology","volume":"1 1","pages":"379-383"},"PeriodicalIF":0.0,"publicationDate":"1963-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90138607","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
It was reported by Gott; and her coworkers (1959), that Vibrio El Tor strain Denken grow in a semisynthetic media consisting of Casamino acids, salts, glucose, vitamins and purine. The author confirmed that Casamino acids in this composition could be substituted with 18 pure amino acids mixture, and tried to simplify the amino acids composition by the elimination test. This strain could grow in a medium containing 8 amino acids, Methionine, Asparatic acid, Leucine, Phenylalanine, Isoleucine, Lysine, Arginine and Glutamic acid. Stain Denken showed moderate growth in this medium, a little less than in 1% Casamino acids medium or 18 amino acids mixture, and in this media 5 serial transfer was possible. Hemolysine production in this medium was approximately on the same level as in 1% Casamino acids medium.
{"title":"Vibrio El Torのアミノ酸要求について","authors":"Taketoshi Arai","doi":"10.3412/JSB.17.371","DOIUrl":"https://doi.org/10.3412/JSB.17.371","url":null,"abstract":"It was reported by Gott; and her coworkers (1959), that Vibrio El Tor strain Denken grow in a semisynthetic media consisting of Casamino acids, salts, glucose, vitamins and purine. The author confirmed that Casamino acids in this composition could be substituted with 18 pure amino acids mixture, and tried to simplify the amino acids composition by the elimination test. This strain could grow in a medium containing 8 amino acids, Methionine, Asparatic acid, Leucine, Phenylalanine, Isoleucine, Lysine, Arginine and Glutamic acid. Stain Denken showed moderate growth in this medium, a little less than in 1% Casamino acids medium or 18 amino acids mixture, and in this media 5 serial transfer was possible. Hemolysine production in this medium was approximately on the same level as in 1% Casamino acids medium.","PeriodicalId":14812,"journal":{"name":"Japanese journal of bacteriology","volume":"04 1","pages":"371-375"},"PeriodicalIF":0.0,"publicationDate":"1962-05-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86293182","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
In a previous study the authors reported that Shigilla infection was experimentally induced in miceby oral inoculation of resistant strains of Shigella. This was accomplished by continuous administration ofantibiotics in combination with pre- and post-inoculation of resistance-lowering agents, such as carbon tetrachloride, which were very useful in the establishment of infection. In this connection the authors showedthat in such an infection, the presence of an increase number of antibiotic-resistant organisms, mainly Escherichia coli, due to the effect of chloramphenicol, disrupts the Shigella population.
{"title":"Experimental Shigella Infections in Mice","authors":"K. Iwata, M. Yoshikawa, H. Motoe","doi":"10.3412/JSB.17.434","DOIUrl":"https://doi.org/10.3412/JSB.17.434","url":null,"abstract":"In a previous study the authors reported that Shigilla infection was experimentally induced in miceby oral inoculation of resistant strains of Shigella. This was accomplished by continuous administration ofantibiotics in combination with pre- and post-inoculation of resistance-lowering agents, such as carbon tetrachloride, which were very useful in the establishment of infection. In this connection the authors showedthat in such an infection, the presence of an increase number of antibiotic-resistant organisms, mainly Escherichia coli, due to the effect of chloramphenicol, disrupts the Shigella population.","PeriodicalId":14812,"journal":{"name":"Japanese journal of bacteriology","volume":"17 1","pages":"434-442"},"PeriodicalIF":0.0,"publicationDate":"1962-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88544930","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A yellow pigment producing organism of Gram-negative, flagellated bacillus was isolated from the blood of a feverous patient. The serum of the patient taken on the fifth disease day showed comparatively high titer (1: 640) in the agglutination test with this organism. On isolation, this organism had possessed Vi-antigen, since the living and heated bacilli or this strain had been agglutinated by anti-Vi sera to full titer, but not by anti-S. typhi 0901 serum. However, on successive subculture on agar, the Vi-antigen was lost in 2 months after isolation and the organism showed reaction with anti-S. typhi 0901 serum. Contray to the first apperance that the organism seemed to be a strain of S. typhi, it was identified as Bact. typhiflavum by its culturai and biochemical properties (Cf. Cruickshank: J. Hyg., 1935, 35, 354). It formed so-called “Symplasmata” discribed by Kathe (1933) on agar slant, especially in the part of condensed water.As to the serological relationship between Bact. typhi-flavum and Salmonella groups, no satisfactory description could be found in the literature. Therefore, an attempt was made to investigate it using the isolated organism and more than 30 different Salmonella serotypes. After the many cross-absorption tests, it was found that this strain of Bact. typhi-flavum possessed such 0-antigens as Salmonella 0122, 13, and 34. Besides, it contained some partial 0-antigens common to such Salmonella serotypes as S. newington, S. albany, S. hoi-sham, S. minnesota and S. tel-aviv, respectively. By slide agglutination tests, the organism showed no reaction with any anti-H standard serum of Salmonella.On the cultivation in bile containg broth, the organism grew easily a variant strain producing no yellow pigment. Serologically this strain was identical to the original strain, but different in the fact that it did not ferment maltose and formed no symplasmata.Although the pathogenicity of the organism for mice was so weak, that on M. L. D. was about 7mg of culture by intraperitoneal inoculation, the present study, together with the literature survey, suggests that Bact. typhi-flavum would cause mild typhoid fever in man depending on conditions.
从发热病人的血液中分离出革兰氏阴性鞭毛杆菌产生黄色色素的有机体。患病第5天采集的患者血清在该菌凝集试验中显示出较高的滴度(1:6 40)。在分离时，该菌具有v抗原，因为该菌株的活菌和加热杆菌可被抗v血清凝集至满效价，而抗s血清则不能。伤寒0901血清。然而，在琼脂上连续继代培养，分离后2个月，vi抗原丢失，生物体表现出与抗s的反应。伤寒0901血清。与最初的表象相反，这种生物似乎是伤寒沙门氏菌的一种菌株，它被确定为Bact。黄斑病的培养和生化特性研究(Cf. Cruickshank: J. Hyg.)。， 1935, 35, 354)。它在琼脂倾斜上形成了Kathe(1933)描述的所谓的“共胞体”，特别是在冷凝水部分。至于Bact的血清学关系。黄斑病和沙门氏菌群，在文献中没有令人满意的描述。因此，我们尝试用分离的菌体和30多种不同的沙门氏菌血清型对其进行研究。经多次交叉吸收试验，发现该菌种具有较强的抗细菌活性。黄斑病具有沙门氏菌0122、13和34等0抗原。此外，它还分别含有纽灵顿沙门氏菌、奥尔巴尼沙门氏菌、海沙姆沙门氏菌、明尼苏达沙门氏菌和特拉维夫沙门氏菌所共有的部分0抗原。经玻片凝集试验，该菌与沙门氏菌抗h标准血清均无反应。在含胆汁的肉汤中培养，该菌很容易生长出不产生黄色素的变异菌株。该菌株在血清学上与原菌株相同，但不同之处在于它不发酵麦芽糖，也不形成联胞体。虽然该菌对小鼠的致病性很弱，在m.l.d.上通过腹腔接种的培养物约为7mg，但本研究结合文献调查表明，Bact。黄斑病会引起轻度伤寒，视情况而定。
{"title":"Vi抗原およびSalmonella菌株と共通のO抗原を有するBacterium typhi-flavumの1菌株","authors":"Seizi Nakazaki","doi":"10.3412/JSB.17.38","DOIUrl":"https://doi.org/10.3412/JSB.17.38","url":null,"abstract":"A yellow pigment producing organism of Gram-negative, flagellated bacillus was isolated from the blood of a feverous patient. The serum of the patient taken on the fifth disease day showed comparatively high titer (1: 640) in the agglutination test with this organism. On isolation, this organism had possessed Vi-antigen, since the living and heated bacilli or this strain had been agglutinated by anti-Vi sera to full titer, but not by anti-S. typhi 0901 serum. However, on successive subculture on agar, the Vi-antigen was lost in 2 months after isolation and the organism showed reaction with anti-S. typhi 0901 serum. Contray to the first apperance that the organism seemed to be a strain of S. typhi, it was identified as Bact. typhiflavum by its culturai and biochemical properties (Cf. Cruickshank: J. Hyg., 1935, 35, 354). It formed so-called “Symplasmata” discribed by Kathe (1933) on agar slant, especially in the part of condensed water.As to the serological relationship between Bact. typhi-flavum and Salmonella groups, no satisfactory description could be found in the literature. Therefore, an attempt was made to investigate it using the isolated organism and more than 30 different Salmonella serotypes. After the many cross-absorption tests, it was found that this strain of Bact. typhi-flavum possessed such 0-antigens as Salmonella 0122, 13, and 34. Besides, it contained some partial 0-antigens common to such Salmonella serotypes as S. newington, S. albany, S. hoi-sham, S. minnesota and S. tel-aviv, respectively. By slide agglutination tests, the organism showed no reaction with any anti-H standard serum of Salmonella.On the cultivation in bile containg broth, the organism grew easily a variant strain producing no yellow pigment. Serologically this strain was identical to the original strain, but different in the fact that it did not ferment maltose and formed no symplasmata.Although the pathogenicity of the organism for mice was so weak, that on M. L. D. was about 7mg of culture by intraperitoneal inoculation, the present study, together with the literature survey, suggests that Bact. typhi-flavum would cause mild typhoid fever in man depending on conditions.","PeriodicalId":14812,"journal":{"name":"Japanese journal of bacteriology","volume":"108 1","pages":"38-47"},"PeriodicalIF":0.0,"publicationDate":"1962-01-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75907233","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"On the Heatresistance of Cl. welchii","authors":"G. Morinaga, J. Yoshizawa","doi":"10.3412/JSB.17.899","DOIUrl":"https://doi.org/10.3412/JSB.17.899","url":null,"abstract":"","PeriodicalId":14812,"journal":{"name":"Japanese journal of bacteriology","volume":"72 1","pages":"899-902"},"PeriodicalIF":0.0,"publicationDate":"1962-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86297829","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The acid-fast bacilli were examined for the capacity of utilizing carbohydrates and organic acids as the carbon sources and sensitivity for antibiotics. The results obtained are asfollows.1. In general, most of the saprophytic strains were capable of utilizing more kinds of carbohydrates as carbon sources than the human or animal strains. The capacity ofutilizing carbohydrates, however, was of little value as criterion in classifying these acid-fast bacilli. Of the substances examined, monosaccharides and and sugar alcohol were utilized by most of the acid-fast strains as carbon sources. On the contrary, disaccharides and organic acids were utilized by only a few of the strains.2. Oxalate was utilized as carbon source only by a type of Mycobacterium smegmatis which is define as an organisms growing at 47°C and failing to survive heating at 60°C for one hour.3. Of 72 strains examined, all were resistant to 100γper ml of para-aminosalicylic acid and 66 to 100γper ml of isoniazed. Colored strains were more sensitive for streptomycin than colorless strains. There was a tendency that the strains which were intermediate betweenthe smooth and the rough type in colonial morphology were strongly resistant to streptomycin.
{"title":"Studies on Non-Pathogenic Acid-Fast Bacilli","authors":"Y. Ochi, Heiji Sato","doi":"10.3412/JSB.16.1068","DOIUrl":"https://doi.org/10.3412/JSB.16.1068","url":null,"abstract":"The acid-fast bacilli were examined for the capacity of utilizing carbohydrates and organic acids as the carbon sources and sensitivity for antibiotics. The results obtained are asfollows.1. In general, most of the saprophytic strains were capable of utilizing more kinds of carbohydrates as carbon sources than the human or animal strains. The capacity ofutilizing carbohydrates, however, was of little value as criterion in classifying these acid-fast bacilli. Of the substances examined, monosaccharides and and sugar alcohol were utilized by most of the acid-fast strains as carbon sources. On the contrary, disaccharides and organic acids were utilized by only a few of the strains.2. Oxalate was utilized as carbon source only by a type of Mycobacterium smegmatis which is define as an organisms growing at 47°C and failing to survive heating at 60°C for one hour.3. Of 72 strains examined, all were resistant to 100γper ml of para-aminosalicylic acid and 66 to 100γper ml of isoniazed. Colored strains were more sensitive for streptomycin than colorless strains. There was a tendency that the strains which were intermediate betweenthe smooth and the rough type in colonial morphology were strongly resistant to streptomycin.","PeriodicalId":14812,"journal":{"name":"Japanese journal of bacteriology","volume":"26 1","pages":"1068-1072"},"PeriodicalIF":0.0,"publicationDate":"1962-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76227526","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Immunoserological relationship between antigenic fractions extracted from Staphylococcus aureus and anti-Staphy. aureus rabbit serum were carried out by sensitized-blood cell haemagglutination reaction and anaphylactic contracting reaction of the isolated guinea pig's ileum passively sensitized with anti-Staphy. aureus rabbit serum. The results obtained were as follows: Antigenic fractions were obtained from Staphy. aureus (A-11) and Staphy. epidermidis (B-12) by the modified method of Verway. By chemical analysis Fraction-A-1 and A-2 was nucleoprotein, Fraction B was protein, Fraction C was polysaccharide and Fraction R was crude nucleoprotein. Coagulase was also prepared by Blobel's method. Tannic acid red cell haemaggulutination reaction was most sensitive when nucleoprotein was used as the sensitized antigen, next crude extraction, purified coagulase, protein and polysaccharide. Cross reactions were also observed with nucleoprotein, polysaccharide and protein derived from Staphy. epidermidis.Remarkable anaphylactic reaction of the isolated intestine of the guinea pig passively sensitized with anti-Staphy. aureus rabbit serum was observed by adding crude extract, protein-fraction or nucleoprotein fraction, but not a reaction was observed with polysaccharide or purified coagulase. However, intestinal anaphylactic reaction was also observed with crude extract and protein-fraction of Staphy. epidermidis. These facts suggest that common antigenicity exists in protein fraction of Staphy.aureus and Staphy. epidermidis.
金黄色葡萄球菌抗原提取物与抗葡萄球菌的免疫血清学关系。采用豚鼠离体回肠被动致敏后的致敏血细胞血细胞凝集反应和过敏收缩反应检测金黄色葡萄球菌兔血清。金黄色兔子血清。结果如下:从Staphy中获得抗原部分。aureus (A-11)和Staphy。通过改良的Verway方法提取表皮细胞(B-12)。经化学分析，a -1和A-2组分为核蛋白，B组分为蛋白质，C组分为多糖，R组分为粗核蛋白。用Blobel法制备凝固酶。以核蛋白为致敏抗原时单宁酸红细胞血凝反应最敏感，其次为粗提物、纯化凝固酶、蛋白和多糖。与核蛋白、多糖及葡萄球菌蛋白均有交叉反应。epidermidis。抗葡萄球菌药被动致敏豚鼠离体肠有明显的过敏反应。用粗提物、蛋白部分或核蛋白部分对金黄色葡萄球菌血清进行观察，但与多糖或纯化凝固酶均无反应。然而，用葡萄树粗提物和蛋白部分也观察到肠道过敏反应。epidermidis。这些事实表明葡萄球菌蛋白部分存在共同的抗原性。aureus and Staphy。epidermidis。
{"title":"Studies on Staphylococcus","authors":"Kôsaku Yoshida, Y. Arai","doi":"10.3412/JSB.16.1042","DOIUrl":"https://doi.org/10.3412/JSB.16.1042","url":null,"abstract":"Immunoserological relationship between antigenic fractions extracted from Staphylococcus aureus and anti-Staphy. aureus rabbit serum were carried out by sensitized-blood cell haemagglutination reaction and anaphylactic contracting reaction of the isolated guinea pig's ileum passively sensitized with anti-Staphy. aureus rabbit serum. The results obtained were as follows: Antigenic fractions were obtained from Staphy. aureus (A-11) and Staphy. epidermidis (B-12) by the modified method of Verway. By chemical analysis Fraction-A-1 and A-2 was nucleoprotein, Fraction B was protein, Fraction C was polysaccharide and Fraction R was crude nucleoprotein. Coagulase was also prepared by Blobel's method. Tannic acid red cell haemaggulutination reaction was most sensitive when nucleoprotein was used as the sensitized antigen, next crude extraction, purified coagulase, protein and polysaccharide. Cross reactions were also observed with nucleoprotein, polysaccharide and protein derived from Staphy. epidermidis.Remarkable anaphylactic reaction of the isolated intestine of the guinea pig passively sensitized with anti-Staphy. aureus rabbit serum was observed by adding crude extract, protein-fraction or nucleoprotein fraction, but not a reaction was observed with polysaccharide or purified coagulase. However, intestinal anaphylactic reaction was also observed with crude extract and protein-fraction of Staphy. epidermidis. These facts suggest that common antigenicity exists in protein fraction of Staphy.aureus and Staphy. epidermidis.","PeriodicalId":14812,"journal":{"name":"Japanese journal of bacteriology","volume":"1 1","pages":"1042-1045"},"PeriodicalIF":0.0,"publicationDate":"1962-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83146407","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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