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Evaluation of erodibility indices and soil properties affected by land-use types in Mbano, south-east Nigeria 尼日利亚东南部Mbano受土地利用类型影响的可蚀性指数和土壤性质评价
Q4 Agricultural and Biological Sciences Pub Date : 2020-01-01 DOI: 10.2298/JAS2004361O
N. Osujieke, N. B. Aririguzo, M. C. Ahukaemere
Soil erosion has been at the forefront of the degradation of soils under different land-use types in southeast Nigeria. Hence, this research aimed to determine the erodibility of the soils under different land-use types in Mbano. The four land-use types selected were oil palm plantation (OP), cassava farm (CF), pineapple orchard (PO) and plantain plantation (PP). However, clay dispersion ratio (CDR), clay dispersion index (CDI) and clay flocculation index (CFI) were the erodibility indices used. A total of 5 composite samples were collected randomly from each type of the land-uses at a depth of 0-20 cm using the free survey. The samples were subjected to laboratory analysis. Data generated were analyzed statistically using a completely randomized design of analyses of variance (ANOVA) and correlation. CDR had an increasing order of 47.9% <48.16% <51.49% <56.24% for soils under CF, PP, PO and OP, while CDI had 36.44%, 29.06%, 40.96% and 49.04% for soils under OP, CF, PO and PP land uses. CFI had 63.62%, 71.24%, 59.14% and 50.96% for soils under OP, CF, PO and PP, respectively. The ANOVA indicated that studied erodibility indices had no significant difference (p=0.05) among the soils under the different land-uses. The research will unite farmers and other land-users in adopting only good conservation practices that will aid the sustainability of the area.
在尼日利亚东南部,土壤侵蚀是不同土地利用类型下土壤退化的主要原因。因此,本研究旨在确定姆巴诺不同土地利用类型下土壤的可蚀性。4种土地利用类型分别为油棕园(OP)、木薯园(CF)、凤梨园(PO)和大蕉园(PP)。粘土分散比(CDR)、粘土分散指数(CDI)和粘土絮凝指数(CFI)是可蚀性指标。采用自由调查的方法,在0 ~ 20 cm的深度内,从每种土地利用类型中随机抽取5个复合样本。这些样品经过实验室分析。产生的数据采用完全随机设计的方差分析(ANOVA)和相关分析进行统计分析。CF、PP、PO和OP土地利用方式下土壤CDR的增加幅度分别为47.9% <48.16% <51.49% <56.24%,而OP、CF、PO和PP土地利用方式下土壤CDI的增加幅度分别为36.44%、29.06%、40.96%和49.04%。OP、CF、PO和PP处理下土壤CFI分别为63.62%、71.24%、59.14%和50.96%。方差分析表明,不同土地利用方式下土壤可蚀性指标无显著差异(p=0.05)。这项研究将使农民和其他土地使用者联合起来,只采取有利于该地区可持续发展的良好保护措施。
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引用次数: 0
Effects of natural antioxidant extract supplementation on the growth performance and meat quality of broiler chickens 添加天然抗氧化提取物对肉鸡生长性能和肉品质的影响
Q4 Agricultural and Biological Sciences Pub Date : 2020-01-01 DOI: 10.2298/JAS2004405I
H. Ishola, Z. Jiya, Y. AdamA., J. Alabi
The study examined the effect of dietary antioxidant supplementation on the performance and meat quality of broiler chickens. Namely, 300 one-day-old Arbor Acres broiler chicks were fed a starter diet from 1 to 4 weeks and a finisher diet for the last 4 weeks. Birds were randomly assigned to treatments based on antioxidant supplementation in drinking water at 0.02% butylated hydroxyanisole (BHA), ordinary water (OW), 0.02% sweet orange peel extract (SOPE), 0.02% shaddock peel extract (SHPE) and 0.02% lemon peel extract (LMPE) per litre of water in a completely randomized design experiment. Feed intake and body weight gain were recorded on a weekly basis. Three birds were selected in each treatment and slaughtered for meat quality determination. BHA and LMPE treatments had the best weight gain and feed conversion efficiency at the significance level (p<0.05). There were significant differences (p<0.05) in the shear force (force peak and yield) in the dietary antioxidants fed to the birds. Sensory parameters (taste, aroma and overall acceptability) show significant differences (p<0.05) amongst the treatments. However, there were no significant differences (p>0.05) in appearance and texture perception amongst treatments. Finally, significant differences (p<0.05) in the lightness (L*) and redness (a*) of the meat samples were observed amongst the treatments. There were no significant differences (p>0.05) in the yellowness (b*) amongst the treatments. It can be concluded that broiler birds fed SOPE, SHPE and LMPE treatments performed better and that these treatments enhanced the meat quality of the birds when compared to BHA and OW treatments.
本试验旨在研究饲粮中添加抗氧化剂对肉鸡生产性能和肉品质的影响。300只1日龄爱拔益加肉鸡雏鸡在第1 ~ 4周饲喂初饲粮,在最后4周饲喂终饲粮。采用完全随机设计的试验方法,按0.02%丁基羟基茴香醚(BHA)、普通水(OW)、0.02%甜橙皮提取物(SOPE)、0.02%柚子皮提取物(SHPE)和0.02%柠檬皮提取物(LMPE)每升水添加抗氧化剂,随机分配给各组。每周记录采食量和体增重。每组取3只,屠宰后测定肉质。在外观和质地感知方面,BHA和LMPE处理的增重和饲料转化率最高,显著水平(p0.05)。黄度(b*)各处理间差异有统计学意义(p0.05)。由此可见,饲喂SOPE、SHPE和LMPE处理的肉鸡肉质优于BHA和OW处理。
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引用次数: 0
Genetic characterization of farmed and wild populations of African catfish (Clarias gariepinus Burchell, 1822) using the random amplified polymorphic marker 非洲鲶鱼(Clarias gariepinus Burchell, 1822)养殖种群和野生种群的随机扩增多态性标记遗传特征分析
Q4 Agricultural and Biological Sciences Pub Date : 2020-01-01 DOI: 10.2298/JAS2004375S
S. Suleiman, M. Diyaware, M. Aliyu, Z. Mohammed
The genetic characterization of Clarias gariepinus was conducted in this study. Thirty (30) C. gariepinus specimens were collected, fifteen (15) each from the wild and farms in northeastern Nigeria for their genetic relatedness and diversity using the RAPD markers. DNA extraction from the blood sample was performed using the Gene Jet Genomic DNA Purification Kit. Five primers were used in employing PCR and amplified 402 RAPD bands from the four strains of Clarias gariepinus. Three hundred and six (76.12%) bands were polymorphic while 86 (21.39%) were monomorphic. The percentage of polymorphism obtained from farmed and wild populations ranged from 58 (47.3%) to 69 (75.9%), respectively. The polymorphic bands per loci within the populations ranged from 67.9% to 82.0%. The mean numbers of inbreeding coefficient (FIS) were 0.083 and 0.053 in the farmed and wild populations. Gene diversity values within farmed and wild populations (Ht) were 0.4522 and 0.4018. The mean genetic differentiation (FST) ranged between (0.203) in the farmed and 0.129 in the wild populations. The analysis of molecular variance revealed that there was 96% of genetic variance within the population and 4% among the population. The genetic identity and distance of four populations were 0.9490 and 0.1038, respectively. The phylogenetic measure has shown that the four strains were divided into two clusters at approximately 0.089 similarity levels. The result indicated a significant level of genetic variation and minimal dendrogram separation in Clarias gariepinus in northeastern Nigeria. Conclusively, this information will be a useful tool for the genetic and breeding program of Clarias gariepinus.
本研究对加里平Clarias gariepinus进行了遗传鉴定。利用RAPD标记对尼日利亚东北部野生和农场的30只加里皮库蚊标本进行遗传亲缘性和多样性分析。使用Gene Jet基因组DNA纯化试剂盒从血样中提取DNA。采用5种引物进行PCR扩增,共扩增出4株加里宾Clarias gariepinus的402条RAPD条带。多态条带306条(76.12%),单态条带86条(21.39%)。养殖群体和野生群体的多态性比例分别为58%(47.3%)~ 69%(75.9%)。每个位点的多态性频带在67.9% ~ 82.0%之间。养殖种群和野生种群的平均近交系数(FIS)分别为0.083和0.053。养殖群体和野生群体的基因多样性值(Ht)分别为0.4522和0.4018。养殖群体的平均遗传分化系数为0.203,野生群体的平均遗传分化系数为0.129。分子变异分析表明,群体内遗传变异占96%,群体间遗传变异占4%。4个居群的遗传同一性和遗传距离分别为0.9490和0.1038。系统发育分析结果表明,4株菌株可分为2个聚类,相似度约为0.089。结果表明,尼日利亚东北部的Clarias gariepinus存在显著的遗传变异和最小的树突分离。这些信息将为该品种的遗传育种提供参考。
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引用次数: 0
Evaluation of integrated nitrogen and phosphorous management using the TT biplot method in soybean TT双标图法评价大豆氮磷综合管理效果
Q4 Agricultural and Biological Sciences Pub Date : 2020-01-01 DOI: 10.2298/jas2001019s
M. Shiri, Morteza Kamrani, Asghar Ebadi
To investigate the effects of integrated nutrient management on oil, protein, grain yield and some traits of soybean, we conducted a factorial experiment involving 4 bio-fertilizer (no inoculation, inoculation with Barvar-2, inoculation with Biosoy and dual inoculation with Biosoy and Barvar-2) and 3 chemical fertilizer levels (no chemical fertilizer, 66 kg ha-1 diammonium phosphates + 50 kg ha-1 urea, 132 kg ha-1 diammonium phosphates + 100 kg ha-1 urea) with 100% chemical fertilizer (200 kg ha-1 diammonium phosphates + 150 kg ha-1 urea) as control base in a randomized complete block with four replications at the research farm of the University of Mohaghegh Ardabili, Iran. Analysis of variance showed that treatment combinations affected most of the traits (P>0.01). We used the biplot analysis as the treatment ? trait (TT) biplot to determine the best treatment combinations and traits. The first two principal components (PC1 and PC2) explained 94 and 96% of the total variant of the standardized data in 2017 and 2018, respectively. Accordingly, application of Biosoy and 150 kg ha-1 urea + 200 kg ha-1 diammonium phosphate significantly increased stem height at harvest, number of grains per plant, biomass, hundred-grain weight, oil and protein yield, protein percent and grain yield compared to the other treatment combinations. The results showed that there were positive correlations between these traits. Also, non-inoculated plants and no chemical fertilizer treatments significantly increased oil percent. The results indicate that higher-yielding treatment combinations had lower oil percent. The biplot was used for ranking of treatment combinations based on a single trait. These study results suggest that bio-fertilizers had a positive influence on soybean and that they could diminish the use of chemical fertilizers. The study reveals that the TT biplot was able to graphically show the interrelationships between traits and support visual comparison of treatments.
为研究综合营养管理对大豆油脂、蛋白质、籽粒产量及部分性状的影响,采用4种生物肥料(不接种、接种Barvar-2、接种Biosoy、双接种Biosoy和Barvar-2)和3种化肥水平(不施用化肥、66 kg ha-1磷酸二铵+ 50 kg ha-1尿素、在伊朗mohaghh Ardabili大学研究农场,采用4个重复的随机完全区试验,以132 kg ha-1磷酸二铵+ 100 kg ha-1尿素为对照,100%化肥(200 kg ha-1磷酸二铵+ 150 kg ha-1尿素)为对照。方差分析表明,组合处理对大部分性状有影响(P>0.01)。我们使用双图分析作为治疗方法。性状(TT)双标图确定最佳处理组合和性状。前两个主成分(PC1和PC2)分别解释了2017年和2018年标准化数据总变异的94%和96%。因此,与其他处理组合相比,施用生物大豆和150 kg ha-1尿素+ 200 kg ha-1磷酸二铵显著提高了收获期茎高、单株粒数、生物量、百粒重、油蛋白产量、蛋白质百分比和籽粒产量。结果表明,这些性状之间存在正相关关系。未接种和不施肥处理显著提高了含油量。结果表明,高产处理组合含油率较低。双标图用于根据单个性状对处理组合进行排序。这些研究结果表明,生物肥料对大豆有积极的影响,可以减少化肥的使用。研究表明,TT双标图能够以图形方式显示性状之间的相互关系,并支持对处理进行视觉比较。
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引用次数: 0
Influence of integrated soil fertility management on the vegetative growth parameters of Zea mays in the guinea savanna eco-zone of Ghana 土壤肥力综合管理对加纳几内亚草原生态区玉米营养生长参数的影响
Q4 Agricultural and Biological Sciences Pub Date : 2020-01-01 DOI: 10.2298/jas2002187a
A. Ammal, A. Abunyewa, E. Yeboah
All over the world, attention has been drawn to the use of eco-friendly biochar application to improve crop productivity. In Ghana, there are available potential feedstocks left unused and can be used for the production of biochar. Therefore, this study investigated the effect of different rates of rice husk biochar and different rates of inorganic nitrogen (N) on the growth of Zea mays in Nyankpala, Northern Ghana. Field experiments were conducted in the cropping period of 2012. The treatments involved 4 different rates of inorganic nitrogen fertilizer (0 kg Nha-1, 30 kg Nha-1, 60 kg Nha-1 and 90 kg Nha-1) and 3 different rates of rice husk biochar (0 ton ha-1, 2 ton ha-1 and 4 ton ha-1). The treatments were allocated in a split-plot design with three replications. The vegetative parameters assessed were the number of leaves, plant height and plant girth. Treatments showed a significant (p<0.05) influence on all the traits considered. The combined effects of inorganic nitrogen fertilizer and rice husk biochar significantly influenced the vegetative growth parameters of Zea mays with the maximum values recorded at 4 ton ha-1rice husk biochar. Yet, this observation corresponds with 2 ton ha-1 rice husk biochar which recorded optimum growth parameters compared to the control. Within the limit of this work, it was concluded that growth parameters of Zea mays in the Guinea Savannah Eco-Zone of Ghana could significantly be improved by applying 4 ton ha-1 rice husk biochar. Integrated soil fertility management (ISFM), a prudent combination of inorganic fertilizers and residues from various sources to sustain the environment is currently a necessity. The experiment revealed that the application of rice husk biochar can improve the growth parameters of Zea mays. Yet, further experiments need to be done using higher rates of rice husk biochar to ensure the appropriate rate of biochar application.
在世界范围内,利用生态友好型生物炭来提高作物生产力已经引起了人们的关注。在加纳,有可利用的未使用的潜在原料,可用于生产生物炭。因此,本研究在加纳北部Nyankpala研究了不同稻壳生物炭用量和不同无机氮用量对玉米生长的影响。田间试验于2012年种植期进行。施用4种不同量的无机氮肥(0 kg hm -1、30 kg hm -1、60 kg hm -1和90 kg hm -1)和3种不同量的稻壳生物炭(0 t hm -1、2 t hm -1和4 t hm -1)。处理被分配为3个重复的分裂地块设计。评估的营养参数为叶片数、株高和株周长。处理对所有性状均有显著影响(p<0.05)。无机氮肥与稻壳生物炭的联合施用显著影响玉米的营养生长参数,在4吨/吨稻壳生物炭时达到最大值。然而,这一观察结果与2吨/公顷稻壳生物炭相对应,与对照相比,它记录了最佳的生长参数。在本研究范围内,施用4吨/公顷的稻壳生物炭可显著改善加纳几内亚大草原生态区玉米的生长参数。综合土壤肥力管理(ISFM),谨慎地结合无机肥料和各种来源的残留物来维持环境,目前是必要的。试验结果表明,稻壳生物炭的施用可以改善玉米的生长参数。然而,进一步的实验需要使用更高的稻壳生物炭用量来确保适当的生物炭用量。
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引用次数: 4
Tendencies of plant production in the Republic of Serbia and at the level NUTS 1 塞尔维亚共和国和NUTS一级植物生产趋势1
Q4 Agricultural and Biological Sciences Pub Date : 2020-01-01 DOI: 10.2298/jas2001069g
Biljana Lj, M. S. Roljevic-Nikolic, V. Subic
In this paper, we analyzed the tendencies of plant production in the Republic of Serbia and at the level of the territorial units Serbia-North and Serbia-South (NUTS 1), for the period 2009-2018 through the indicators of the coverage of areas and an average yield of 28 plant crops. The aim of the paper is to point out the differences in the representation of areas under different types of crops and to indicate the degree of their agreement between the mentioned territorial units by Spearman's correlation coefficient. For the analysis of the observed indicators, the methods of descriptive statistics, as well as Spearman's correlation coefficient, were used. The data indicate that the Serbia-North region is dominated by areas under cereals, industrial crops, peas and peaches, while the Serbia-South region is covered by areas under fodder crops, vegetable crops (potatoes, tomatoes, peppers, onion, garlic, beans, cucumber, melons and watermelons) and perennial crops. The average yields of the observed crops differ significantly between the regions of Serbia-North and Serbia-South. Spearmen's correlation coefficient of the indicators of the coverage of areas indicated a high degree of stacking of areas under wheat and tobacco (0.927**), as well as areas under lucerne and clover with areas under fruit plantations. Also, a high degree of stacking of areas under maize and peas (0.798**) was established, but also between areas under sunflower and soya (0.891**).
在本文中,我们通过28种植物作物的面积覆盖和平均产量指标,分析了2009-2018年塞尔维亚共和国以及塞尔维亚北部和塞尔维亚南部领土单位(NUTS 1)的植物生产趋势。本文的目的是指出不同作物类型下的区域表示的差异,并通过Spearman相关系数表示上述领土单位之间的一致程度。对观测指标的分析,采用描述性统计方法和Spearman相关系数。数据表明,塞尔维亚北部地区主要种植谷物、工业作物、豌豆和桃子,而塞尔维亚南部地区则种植饲料作物、蔬菜作物(土豆、西红柿、辣椒、洋葱、大蒜、豆类、黄瓜、瓜和西瓜)和多年生作物。观察到的作物的平均产量在塞尔维亚北部和塞尔维亚南部地区之间差别很大。各面积盖度指标的Spearmen相关系数表明,小麦和烟草种植面积(0.927**)、苜蓿和三叶草种植面积与果树种植面积的叠加程度较高。玉米和豌豆种植面积之间存在较高的堆积度(0.798**),向日葵和大豆种植面积之间存在较高的堆积度(0.891**)。
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引用次数: 3
Effects of spatial arrangement and population density on the growth and yield of sesame (Sesamum indicum L.) in a sesame/maize intercrop 空间布局和种群密度对芝麻/玉米间作芝麻生长和产量的影响
Q4 Agricultural and Biological Sciences Pub Date : 2020-01-01 DOI: 10.2298/JAS2004337I
A. Isaac, K. Oyebisi, S. Kayode, S. A. Mojisola
A field experiment was carried out at the Teaching and Research Farm, Kwara State University, Malete, Nigeria. The aim of the experiment was to investigate the growth and yield of sesame (Sesamum indicum L.) as affected by the row arrangement and population density of maize (Zea mays L.). The full population of sesame in two-row arrangements (1:1) and (2:2) was combined with four population densities of maize viz: 100S:100M; 100S:75M; 100S:50M and 100S:25M (where S and M represented sesame and maize, respectively). Sole crops of sesame and maize at full population were included in the treatments as control. The number of pods per plant (NPP), length of fruit zone (LFZ), and yield of sesame were significantly (P?0.5) influenced by the interactive effect of population ratios and row arrangements. These variables increased as the population of associated maize decreased. All variables measured in maize were influenced by population density and row arrangement except for the number of cobs per plant (NCP), cob length (CL), and cob circumference (CC). Regardless of spatial arrangement and population density, the aggressivity (A) value was positive for sesame and negative for maize. The competitive ratio (CR) values were also higher in sesame than in maize. Land equivalent ratio (LER) and land equivalent coefficient (LEC) values, for all population ratios tested, indicated the intercropping advantage with the highest value recorded at a full population of sesame mixed with fifty percent population of maize in a 2:2-row arrangement and hence, recommended for adoption.
在尼日利亚马勒特Kwara州立大学教学和研究农场进行了实地试验。本试验旨在研究玉米行距和种群密度对芝麻生长和产量的影响。以2排(1:1)和2排(2:2)的芝麻满居群与玉米4个居群密度组合,分别为:1000s和100M;100年代:75;100S:50M, 100S:25M(其中S代表芝麻,M代表玉米)。以满居的芝麻和玉米单作作对照。群体比例和行距对单株荚果数(NPP)、果区长(LFZ)和产量的交互效应显著(P?0.5)。这些变量随着伴生玉米种群的减少而增加。除单株穗轴数(NCP)、穗轴长(CL)和穗轴周长(CC)外,玉米测定的所有变量均受种群密度和行距的影响。无论空间布局和种群密度如何,芝麻的侵略性(A)值为正,玉米为负。芝麻的竞争比(CR)值也高于玉米。所有群体比例的土地等效比(LER)和土地等效系数(LEC)值均表明,以2∶2行种植方式,芝麻满居与玉米占50%居混合时具有最高的间作优势,因此推荐采用。
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引用次数: 7
A review: Natural and artificial radionuclides and radiation hazard parameters in the soil of mountain regions in Serbia 综述:塞尔维亚山区土壤中天然和人工放射性核素及辐射危害参数
Q4 Agricultural and Biological Sciences Pub Date : 2020-01-01 DOI: 10.2298/jas2001001m
B. Mitrović, D. Todorovic, J. Ajtić, Borjana Vranjes
This review paper discusses the content of natural (40K, 238U, 226Ra, and 232Th) and artificial (137Cs) radionuclides in the soil of the mountains of Maljen, Tara and Kopaonik in the Republic of Serbia over 2002-2015. In addition, the paper gives radiation hazard parameters, i.e., radium equivalent activity, absorbed dose rate, annual effective dose equivalent, external hazard index, annual gonadal dose equivalent, and excess lifetime cancer risk outdoors that we calculated from the obtained content of the natural radionuclides in the soil samples. We compared the parameters to previously published results for different parts of the country and looked into the radioecological status of the investigated areas.
本文讨论了2002-2015年塞尔维亚共和国马尔詹、塔拉和科波奥尼克山区土壤中天然(40K、238U、226Ra和232Th)和人工(137Cs)放射性核素的含量。此外,本文给出了辐射危害参数,即镭当量活度、吸收剂量率、年有效剂量当量、外部危害指数、年性腺剂量当量和室外过量终身癌症风险,由土壤样品中获得的天然放射性核素含量计算得出。我们将这些参数与先前公布的全国不同地区的结果进行了比较,并研究了被调查地区的放射生态学状况。
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引用次数: 1
The importance of crop rotation in intensive vegetable production in a greenhouse 轮作在温室集约化蔬菜生产中的重要性
Q4 Agricultural and Biological Sciences Pub Date : 2020-01-01 DOI: 10.2298/jas2003199c
J. Červenski, S. Medic-Pap, D. Danojević, A. Savić, D. Bugarski
Intensive vegetable production nowadays is a large ?energy consumer?. Producers look only at the economic side of such production, which is most often the excuse for its current realization. Intensive vegetable production is now reduced to cultivating several vegetable species, more often in the single-crop system. By thinking this way we bring the entire production into an unsustainable situation. Therefore, vegetable production in a greenhouse should be organized by growing preceding crops, main crops and stubble crops. Intensive vegetable production implies the maximally well-organized use of available land and resources. This includes proper crop rotation and cultivation practices, as well as knowledge of the market, as a possibility of placing excess production. With the good organization of crop rotation and the timely replacement of crops, we can transform a greenhouse into a useful place for vegetable production.
如今,集约化蔬菜生产是一个耗能大户。生产者只关注这种生产的经济方面,这是目前实现这种生产的最常见的借口。集约化蔬菜生产现在减少到种植几种蔬菜,更经常是在单一作物系统中。通过这种方式思考,我们把整个生产带入了一个不可持续的局面。因此,温室蔬菜生产应按种植前茬作物、主茬作物和茬作物来组织。集约化蔬菜生产意味着最大限度地合理利用可利用的土地和资源。这包括适当的作物轮作和耕作方法,以及对市场的了解,作为放置过剩产量的可能性。组织好轮作,及时换种,就可以把大棚改造成蔬菜生产的有用场所。
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引用次数: 0
Histopathology of Colletotrichum sp. in infected mango (Magnifera indica L.) fruits 芒果(Magnifera indica L.)果实炭疽病菌的组织病理学
Q4 Agricultural and Biological Sciences Pub Date : 2020-01-01 DOI: 10.2298/JAS2004351O
O. Oladele
Postharvest losses as a result of anthracnose infection remain a serious threat to mango producers. Hence, histopathology of mango fruits after being artificially infected with spores of Colletotrichum sp. was investigated. Fruits at the physiologically mature stage were wounded (cut) in the peels and inoculated with a spore suspension of Colletotrichum sp. (8.04 ? 103 conidia m1-1) and incubated at 28?2?C for five days to allow pathogen establishment. The infected peel was then carefully cut with a razor blade and dehydrated in series in different grades (50, 70, 80, 90 and 100%) of ethyl alcohol for 1? hours each. Histopathological studies were carried out on the infected peel tissue excised from inoculated fruits using standard procedures while unwounded peels of fruits that were not artificially inoculated served as control. Sections were examined by light microscopy to observe histopathological differences between the infected and non-infected fruits. Results from this study revealed that only the wounded peel showed symptoms of anthracnose infection as a result of the artificial inoculation, but the unwounded peel showed no disease symptoms. This showed that the fungus infected the mango fruits through the peel wounds. Besides, the disorganization of the cells and the rupture of the cell walls were observed microscopically, thus indicating disease establishment in the infected fruits. Therefore, mango producers should avoid mechanical damage to fruits during harvesting since this work confirms that the fungus infects mango fruits through wounds.
由于炭疽病感染而造成的采后损失仍然是对芒果生产者的严重威胁。因此,对人工感染炭疽菌孢子后芒果果实的组织病理学进行了研究。生理成熟期的果实在果皮上伤(切),用炭疽菌孢子悬悬液(8.04 ?103分生孢子m1-1),在28?2?放置5天,让病原体滋生。然后用剃须刀片小心地切开受感染的果皮,并按不同等级(50、70、80、90和100%)分别脱水1?每小时。组织病理学研究采用标准程序从接种的水果上切除受感染的果皮组织,而未人工接种的未受伤的果皮作为对照。切片在光镜下观察感染和未感染果实的组织病理学差异。本研究结果显示,人工接种后,只有伤皮出现炭疽病感染症状,而未伤皮无病症状。这表明真菌是通过芒果果皮伤口感染芒果果实的。此外,在显微镜下观察到细胞的破坏和细胞壁的破裂,从而表明感染果实的疾病已经建立。因此,芒果生产者应避免在收获过程中对水果造成机械损伤,因为这项工作证实了真菌通过伤口感染芒果果实。
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Journal of Agricultural Sciences, Belgrade
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