Pub Date : 2023-12-30DOI: 10.55218/jasr.2023141101
Mahesh B. Narkhede, Pavan P. Chinchole, Mangesh N Deokar, Kiran P Gaikwad, Ananta G Titare, Sonali P Mahajan, Nikhil G Ratnaparkhi
Animal models have provided invaluable information in biological evaluation and drug screening. It comes to predicting the human body’s response to candidate drugs; traditional laboratory animal models are woefully inadequate. However, increased concern for the welfare of the animals used, and a growing awareness of the concept of animal rights, has brought a greater focus on the related ethical issues.In this review, we seek to summarize the important anatomical aspects of Zebra fish as it is used as an alternative animal model for drug screening.
{"title":"ZEBRA FISH: AN EXPERIMENTAL MODEL FOR DRUG SCREENING","authors":"Mahesh B. Narkhede, Pavan P. Chinchole, Mangesh N Deokar, Kiran P Gaikwad, Ananta G Titare, Sonali P Mahajan, Nikhil G Ratnaparkhi","doi":"10.55218/jasr.2023141101","DOIUrl":"https://doi.org/10.55218/jasr.2023141101","url":null,"abstract":"Animal models have provided invaluable information in biological evaluation and drug screening. It comes to predicting the human body’s response to candidate drugs; traditional laboratory animal models are woefully inadequate. However, increased concern for the welfare of the animals used, and a growing awareness of the concept of animal rights, has brought a greater focus on the related ethical issues.In this review, we seek to summarize the important anatomical aspects of Zebra fish as it is used as an alternative animal model for drug screening.","PeriodicalId":14906,"journal":{"name":"Journal of Advanced Scientific Research","volume":" 52","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139139573","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-11-30DOI: 10.55218/jasr.2023141001
P. Pravalika, G. Jephthah, A. R. Reddy, T. R. Rao
Development and validation of simple, rapid, accurate, precise and sensitive UV-Spectrophotometric method for the estimation of Ephedrine hydrochloride in bulk drug and Injection dosage form was performed in the current research. Quantitative determination of Ephedrine hydrochloride was done using distilled water as a solvent. λmax of Ephedrine hydrochloride in distilled water was measured at 270 nm. Linearity range for Ephedrine hydrochloride was 2-10 μg/mL and coefficient of correlation for Ephedrine hydrochloride was 0.999. Accuracy was performed and the percentage recovery of Ephedrine hydrochloride was found to be in the range of 98.6-99.17. The % relative standard deviation (RSD) for precision was less than 2%, LOD & LOQ was 0.079 μg/mL and 0.24 μg/mL respectively. The results suggest that method can be employed for routine analysis of Ephedrine hydrochloride in bulk drug and in pharmaceutical Dosage form.
{"title":"METHOD DEVELOPMENT AND VALIDATION OF UV SPECTROSCOPY FOR THE ESTIMATION OF EPHEDRINE HYDROCHLORIDE IN BULK AND PHARMACEUTICAL FORMULATION","authors":"P. Pravalika, G. Jephthah, A. R. Reddy, T. R. Rao","doi":"10.55218/jasr.2023141001","DOIUrl":"https://doi.org/10.55218/jasr.2023141001","url":null,"abstract":"Development and validation of simple, rapid, accurate, precise and sensitive UV-Spectrophotometric method for the estimation of Ephedrine hydrochloride in bulk drug and Injection dosage form was performed in the current research. Quantitative determination of Ephedrine hydrochloride was done using distilled water as a solvent. λmax of Ephedrine hydrochloride in distilled water was measured at 270 nm. Linearity range for Ephedrine hydrochloride was 2-10 μg/mL and coefficient of correlation for Ephedrine hydrochloride was 0.999. Accuracy was performed and the percentage recovery of Ephedrine hydrochloride was found to be in the range of 98.6-99.17. The % relative standard deviation (RSD) for precision was less than 2%, LOD & LOQ was 0.079 μg/mL and 0.24 μg/mL respectively. The results suggest that method can be employed for routine analysis of Ephedrine hydrochloride in bulk drug and in pharmaceutical Dosage form.","PeriodicalId":14906,"journal":{"name":"Journal of Advanced Scientific Research","volume":" 13","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-11-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139197149","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-11-30DOI: 10.55218/jasr.2023141007
Arya Raveendran, Asha P N
Rasatarangini, one of the esteemed treatises of Ayurvediya Rasasastra has mentioned numerous malahara (ointment) preparations with herbal and herbo-mineral origin. The term malahara itself mean those which remove mala (impurities) from vrana (ulcers), vidradhi (abscesses), twak vikara (skin diseases) etc. Acharya Sadananda Sharma, author of Rasatarangini has mentioned Sindhooradhya malahara a preparation with potent action on vranashodhana ropana (wound cleansing and healing) and bhootasanga prashamana (antimicrobial) properties. Acharya has mentioned two malahara preparations named as Prathama and Dwiteeya Sindhooradhya malahara with only difference in single drug. Both preparations are indicated in skin manifestations like pama and kandu, which may be due to invasion of some external organisms or microbes. WHO has declared that antimicrobial resistance is one of the top 10 global public health threat facing humanity and thus the search for ayurvedic antimicrobials are progressing day by day. Since it is a topical antibiotic preparation, with very minimal ingredients proving its efficacy will be of great use for the society. The present study aims at the preparation and comparison between the antimicrobial activity of the two Sindhooradhya malahara named as Prathama and Dwiteeya respectively. The main findings are, antimicrobial study by both agar well diffusion and macro broth dilution showed that more action in Prathama Sindhooradhya malahara. Among the 4 tested strains, significant results are seen in case ofStreptococcus pyogenes and Candida albicans whereas Dwiteeya Sindhooradhya malahara had no action in the tested strains of microbes.
{"title":"A COMPARATIVE STUDY ON PRATHAMA AND DWITEEYA SINDHOORADHYA MALAHARA WITH SPECIAL REFERENCE TO ITS ANTIMICROBIAL ACTIVITY","authors":"Arya Raveendran, Asha P N","doi":"10.55218/jasr.2023141007","DOIUrl":"https://doi.org/10.55218/jasr.2023141007","url":null,"abstract":"Rasatarangini, one of the esteemed treatises of Ayurvediya Rasasastra has mentioned numerous malahara (ointment) preparations with herbal and herbo-mineral origin. The term malahara itself mean those which remove mala (impurities) from vrana (ulcers), vidradhi (abscesses), twak vikara (skin diseases) etc. Acharya Sadananda Sharma, author of Rasatarangini has mentioned Sindhooradhya malahara a preparation with potent action on vranashodhana ropana (wound cleansing and healing) and bhootasanga prashamana (antimicrobial) properties. Acharya has mentioned two malahara preparations named as Prathama and Dwiteeya Sindhooradhya malahara with only difference in single drug. Both preparations are indicated in skin manifestations like pama and kandu, which may be due to invasion of some external organisms or microbes. WHO has declared that antimicrobial resistance is one of the top 10 global public health threat facing humanity and thus the search for ayurvedic antimicrobials are progressing day by day. Since it is a topical antibiotic preparation, with very minimal ingredients proving its efficacy will be of great use for the society. The present study aims at the preparation and comparison between the antimicrobial activity of the two Sindhooradhya malahara named as Prathama and Dwiteeya respectively. The main findings are, antimicrobial study by both agar well diffusion and macro broth dilution showed that more action in Prathama Sindhooradhya malahara. Among the 4 tested strains, significant results are seen in case ofStreptococcus pyogenes and Candida albicans whereas Dwiteeya Sindhooradhya malahara had no action in the tested strains of microbes.","PeriodicalId":14906,"journal":{"name":"Journal of Advanced Scientific Research","volume":"5 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-11-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139200058","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-11-30DOI: 10.55218/jasr.2023141006
Amith T, P.S. Sujatha
Nephrolithiasis, commonly known as kidney stone disease or urolithiasis, is a crystallopathy in which a solid mass forms in the urinary tract. The aim of the present research is to evaluate the way that albino rats administered with a high-protein diet, react to methanolic root extracts of Lagerstroemia speciosa anticalciuric properties. Medicinal plants are frequently utilised in folk medicine to treat a wide range of illnesses. Banaba is the common name for L. speciosa, a member of the Lythraceae family; Poomaruthu is its Tamil name. The test animals were given a diet high in protein and low in protein as well as 45 days of treatment with methanolic root extracts of L. speciosa. The experiment was scheduled with a sample collection, biochemical inquiries, and histopathological exams. The experiment had been scheduled with a sample collection, biochemical investigations, and histopathological evaluations. The biochemical data reveal that as compared to the control, III, and V groups, the low-protein diet groups II and IV had greater blood urea concentrations. The low-protein diet groups II and IV exhibited an increased blood creatinine concentration in contrast to the control, III, and V groups, while group V (H.P+MRE 500 mg/kg) demonstrated a nearly identical outcome to that of the control groups. L. speciosa root extracts in methanol are given to groups to control creatinine concentration. The normal control group's histopathological analysis revealed tubules with a single epithelial lining along the outermost layer that were normal in size.
肾结石俗称肾石症或泌尿系结石，是一种在尿路中形成固体块状物的结晶病。本研究的目的是评估以高蛋白饮食喂养的白化大鼠对 Lagerstroemia speciosa 的甲醇根萃取物抗肾结石特性的反应。药用植物在民间医学中经常被用来治疗各种疾病。Banaba 是枸杞科植物 L. speciosa 的俗名；Poomaruthu 是其泰米尔语名称。试验动物分别摄入高蛋白和低蛋白饮食，并接受为期 45 天的 L. speciosa 根甲醇提取物治疗。实验安排了样本采集、生化检查和组织病理学检查。实验安排了样品采集、生化调查和组织病理学评估。生化数据显示，与对照组、III 组和 V 组相比，低蛋白饮食 II 组和 IV 组的血尿素浓度更高。与对照组、III 组和 V 组相比，低蛋白饮食 II 组和 IV 组的血肌酐浓度升高，而 V 组（H.P+MRE 500 毫克/千克）的结果与对照组几乎相同。第五组（H.P+MRE 500 毫克/千克）的结果与对照组几乎相同。正常对照组的组织病理学分析显示，肾小管最外层有单层上皮衬里，大小正常。
{"title":"ANTICALCIURIA EFFECT OF METHANOLIC ROOT EXTRACT OF LAGERSTROEMIA SPECIOSA (L). PERS (LYTHRACEAE) AGAINST HIGH PROTEIN DIET INGESTED IN ALBINO RATS","authors":"Amith T, P.S. Sujatha","doi":"10.55218/jasr.2023141006","DOIUrl":"https://doi.org/10.55218/jasr.2023141006","url":null,"abstract":"Nephrolithiasis, commonly known as kidney stone disease or urolithiasis, is a crystallopathy in which a solid mass forms in the urinary tract. The aim of the present research is to evaluate the way that albino rats administered with a high-protein diet, react to methanolic root extracts of Lagerstroemia speciosa anticalciuric properties. Medicinal plants are frequently utilised in folk medicine to treat a wide range of illnesses. Banaba is the common name for L. speciosa, a member of the Lythraceae family; Poomaruthu is its Tamil name. The test animals were given a diet high in protein and low in protein as well as 45 days of treatment with methanolic root extracts of L. speciosa. The experiment was scheduled with a sample collection, biochemical inquiries, and histopathological exams. The experiment had been scheduled with a sample collection, biochemical investigations, and histopathological evaluations. The biochemical data reveal that as compared to the control, III, and V groups, the low-protein diet groups II and IV had greater blood urea concentrations. The low-protein diet groups II and IV exhibited an increased blood creatinine concentration in contrast to the control, III, and V groups, while group V (H.P+MRE 500 mg/kg) demonstrated a nearly identical outcome to that of the control groups. L. speciosa root extracts in methanol are given to groups to control creatinine concentration. The normal control group's histopathological analysis revealed tubules with a single epithelial lining along the outermost layer that were normal in size.","PeriodicalId":14906,"journal":{"name":"Journal of Advanced Scientific Research","volume":"45 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-11-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139203128","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-11-30DOI: 10.55218/jasr.2023141005
S. Patil, P. M. Biradar
The cavity of earthworm is filled with transparent fluid called coelomic fluid which is derived from the mesenchymal lining, exhibits a wide variety of biological activities. The coleomic fluid consists of various cells and many biomolecular components involved in innate immunity. This study aims to highlight the use of various standard methods for the extraction of coelomic fluid and also to calculate the differential coelomocytes count of three epigeic earthworm species such as Eudrilus eugeniae (EE), Eisenia fetida (EF) and Perioynx excavatus (PE). In present study, cold shock method was used for extraction of coelomic fluid from different epigeic earthworm species as it is more advantages than the other methods, especially for earthworms. During the study, four different kinds of coelomocytes were witnessed such as amoebocytes, chloragogen cells, mucocytes and circular cells. The total coelomocytes count was somewhat equal in allthree species with no significant difference, whereas a significant difference was noticed in differential count of coelomocytes i.e. Among all the coelomocytes amoebocytes, chloragogen cell and mucocytes were more in EE (56.33±0.33, 37.00±0.57 and 3.66±0.33) followed by PE (31.00±0.57, 16.33±0.33 and 2.00±0.57) and less in EF (24.00±0.32, 8.00±0.21 and 2.00±0.33) whereas circular cells were more in EF ( 66.00±0.57) followed by PE (50.66±0.33) and less in EE (2.66±0.33).
{"title":"The COELOMIC FLUID EXTRACTION AND COELOMOCYTES COUNT IN DIFFERENT EPIGEIC EARTHWORMS","authors":"S. Patil, P. M. Biradar","doi":"10.55218/jasr.2023141005","DOIUrl":"https://doi.org/10.55218/jasr.2023141005","url":null,"abstract":"The cavity of earthworm is filled with transparent fluid called coelomic fluid which is derived from the mesenchymal lining, exhibits a wide variety of biological activities. The coleomic fluid consists of various cells and many biomolecular components involved in innate immunity. This study aims to highlight the use of various standard methods for the extraction of coelomic fluid and also to calculate the differential coelomocytes count of three epigeic earthworm species such as Eudrilus eugeniae (EE), Eisenia fetida (EF) and Perioynx excavatus (PE). In present study, cold shock method was used for extraction of coelomic fluid from different epigeic earthworm species as it is more advantages than the other methods, especially for earthworms. During the study, four different kinds of coelomocytes were witnessed such as amoebocytes, chloragogen cells, mucocytes and circular cells. The total coelomocytes count was somewhat equal in allthree species with no significant difference, whereas a significant difference was noticed in differential count of coelomocytes i.e. Among all the coelomocytes amoebocytes, chloragogen cell and mucocytes were more in EE (56.33±0.33, 37.00±0.57 and 3.66±0.33) followed by PE (31.00±0.57, 16.33±0.33 and 2.00±0.57) and less in EF (24.00±0.32, 8.00±0.21 and 2.00±0.33) whereas circular cells were more in EF ( 66.00±0.57) followed by PE (50.66±0.33) and less in EE (2.66±0.33).","PeriodicalId":14906,"journal":{"name":"Journal of Advanced Scientific Research","volume":"64 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-11-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139200548","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Anaemia is a major public health issue in India. As per the National Health Family Survey (NFHS), the prevalence of anaemia has increased (55.8% to 59.1%) from NFHS-4 to NFHS-5. Anaemia among adolescent girls is higher (56% to 59%) than among boys (30% to 31%). According to NFHS 5, infant and child mortality rates have improved since the previous round; therefore, attention is required for adolescents because they are future mothers. It is relevant to bring out the prevalence of anaemia in adolescent girls to prevent maternal and child health in the future. This cross-sectional study was conducted among 250 adolescent girls in Munirika urban village, Delhi. 60.4% of anaemia cases were found among 250 study participants. The study revealed that anaemia was highly prevalent among adolescent girls. Anaemia was associated with less literate, lower starta, poor diet and underweight factors. Anaemia was found to be significantly more common in adolescent girls, and this incidence was higher in lower socio-economic strata, parents who were less educated, poor diet habits, and underweight causes. To have an early impact on reducing adolescents' anaemia, school teachers and Frontline Health Workers (FHWs) should educate and counsel them during home visits. There is a need for IFA supplementation and regular diet pattern awareness among adolescent girls. Interpersonal counseling needs to be strengthened at the community level through home visits and initiating awareness programs. The nutritional status of adolescent girls can be improved through counseling and health education. T3 camps should be held at the school level to raise awareness of anaemia among adolescent girls.
{"title":"PREVALENCE OF ANAEMIA AMONG ADOLESCENT GIRLS: A COMMUNITY-BASED STUDY IN DELHI, INDIA","authors":"Rashmi Kumari, Ajit kumar Lenka, Sidharth Kumar, Malvika Lodhi","doi":"10.55218/jasr.2023141002","DOIUrl":"https://doi.org/10.55218/jasr.2023141002","url":null,"abstract":"Anaemia is a major public health issue in India. As per the National Health Family Survey (NFHS), the prevalence of anaemia has increased (55.8% to 59.1%) from NFHS-4 to NFHS-5. Anaemia among adolescent girls is higher (56% to 59%) than among boys (30% to 31%). According to NFHS 5, infant and child mortality rates have improved since the previous round; therefore, attention is required for adolescents because they are future mothers. It is relevant to bring out the prevalence of anaemia in adolescent girls to prevent maternal and child health in the future. This cross-sectional study was conducted among 250 adolescent girls in Munirika urban village, Delhi. 60.4% of anaemia cases were found among 250 study participants. The study revealed that anaemia was highly prevalent among adolescent girls. Anaemia was associated with less literate, lower starta, poor diet and underweight factors. Anaemia was found to be significantly more common in adolescent girls, and this incidence was higher in lower socio-economic strata, parents who were less educated, poor diet habits, and underweight causes. To have an early impact on reducing adolescents' anaemia, school teachers and Frontline Health Workers (FHWs) should educate and counsel them during home visits. There is a need for IFA supplementation and regular diet pattern awareness among adolescent girls. Interpersonal counseling needs to be strengthened at the community level through home visits and initiating awareness programs. The nutritional status of adolescent girls can be improved through counseling and health education. T3 camps should be held at the school level to raise awareness of anaemia among adolescent girls.","PeriodicalId":14906,"journal":{"name":"Journal of Advanced Scientific Research","volume":"43 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-11-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139209019","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-11-30DOI: 10.55218/jasr.2023141008
Annie Swathisha, Kiran Kumar N, Savitha B Naik, Preetham Hs, Shylaja V
Adhesion to dentin remains challenging due to its complex nature that prevents complete infiltration of hydrophobic monomer to hydrophilic dentinal substrate. Recently, Er:YAG laser has been used in dentin pretreatment to enhance the bond strength. A deficient hybrid layer is formed following laser etching as it causes thermal damage and collagen denaturation of dentinal substrate. As the results are controversial, attempts are made to modify laser etching technique with respect to energy density, pulse duration and water spray. Thus, the present study aimed to evaluate the influence of Er:YAG laser conditioning of dentin by activation of Chlorhexidine collagen stabilising agent on the bond strength. Samples included 60 freshly extracted premolar which were divided into three groups (n=20) based on the protocol of dentin pretreatment done: Group 1 (Control) - Phosphoric acid etching followed by universal adhesive; Group 2 (Experimental) - Conventional Er:YAG laser dentioning; Group3 (Experimental) - Er:YAG laser dentin conditioning with coronal reservoir of 0.12% Chlorhexidine. Occlusal class 1 cavity was prepared on the samples followed by which dentin conditioning protocol was carried out for the respective groups. Universal adhesive was applied followed by Composite resin restoration. 1000 cycles of Thermocycling was done and the samples were sectioned to obtain microsections. Microtensile bond strength values were obtained by subjecting the specimens to Universal testing machine.Highest microtensile bond strength value was obtained for group 1 followed by group 3 with least value obtained for Group2 with statistically significant values between the groups. (p<0.0001). Within the limitations of the present invitro study, it can be concluded that preetching with phosphoric acid increases the bond strength of universal adhesive to dentin. Laser conditioning of dentin through activation of chlorhexidine provided better bond strength value of universal adhesive when compared to conventional laser dentin conditioning.
{"title":"MICROTENSILE BOND STRENGTH OF UNIVERSAL ADHESIVE TO DENTIN AFTER ER: YAG LASER PRETREATMENT WITH DIFFERENT CONDITIONING MODES - AN IN VITRO STUDY","authors":"Annie Swathisha, Kiran Kumar N, Savitha B Naik, Preetham Hs, Shylaja V","doi":"10.55218/jasr.2023141008","DOIUrl":"https://doi.org/10.55218/jasr.2023141008","url":null,"abstract":"Adhesion to dentin remains challenging due to its complex nature that prevents complete infiltration of hydrophobic monomer to hydrophilic dentinal substrate. Recently, Er:YAG laser has been used in dentin pretreatment to enhance the bond strength. A deficient hybrid layer is formed following laser etching as it causes thermal damage and collagen denaturation of dentinal substrate. As the results are controversial, attempts are made to modify laser etching technique with respect to energy density, pulse duration and water spray. Thus, the present study aimed to evaluate the influence of Er:YAG laser conditioning of dentin by activation of Chlorhexidine collagen stabilising agent on the bond strength. Samples included 60 freshly extracted premolar which were divided into three groups (n=20) based on the protocol of dentin pretreatment done: Group 1 (Control) - Phosphoric acid etching followed by universal adhesive; Group 2 (Experimental) - Conventional Er:YAG laser dentioning; Group3 (Experimental) - Er:YAG laser dentin conditioning with coronal reservoir of 0.12% Chlorhexidine. Occlusal class 1 cavity was prepared on the samples followed by which dentin conditioning protocol was carried out for the respective groups. Universal adhesive was applied followed by Composite resin restoration. 1000 cycles of Thermocycling was done and the samples were sectioned to obtain microsections. Microtensile bond strength values were obtained by subjecting the specimens to Universal testing machine.Highest microtensile bond strength value was obtained for group 1 followed by group 3 with least value obtained for Group2 with statistically significant values between the groups. (p<0.0001). Within the limitations of the present invitro study, it can be concluded that preetching with phosphoric acid increases the bond strength of universal adhesive to dentin. Laser conditioning of dentin through activation of chlorhexidine provided better bond strength value of universal adhesive when compared to conventional laser dentin conditioning.","PeriodicalId":14906,"journal":{"name":"Journal of Advanced Scientific Research","volume":"324 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-11-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139203618","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The leaves of the plant Dryopteris marginalis collected from Kumaon District, Nainital India, were analyzed for the presence of different phytochemicals. The aim of this study is to screen the biologically active compounds; alkaloids, glycosides, tannins, flavonoids, steroids, saponins, phenolic compounds, fat and oils, reducing and non-reducing sugar and amino acids. Phytochemical screening indicated the presence of flavonoids, phenolic compounds, steroids, saponins, alkaloids, amino acids, volatile oils, reducing and hexose sugar. The phytochemical composition of the leaves of the plants indicates their medicinal properties.
{"title":"PHYTOCHEMICAL SCREENING, GAS CHROMATOGRAPHY-MASS SPECTROMETRY, AND IN VITRO ANTIOXIDANT POTENTIAL OF DRYOPTERIS MARGINALIS","authors":"Akhil Gupta, Rakesh Sharma, Neeraj Kumar, Mayank Bansal","doi":"10.55218/jasr.2023141004","DOIUrl":"https://doi.org/10.55218/jasr.2023141004","url":null,"abstract":"The leaves of the plant Dryopteris marginalis collected from Kumaon District, Nainital India, were analyzed for the presence of different phytochemicals. The aim of this study is to screen the biologically active compounds; alkaloids, glycosides, tannins, flavonoids, steroids, saponins, phenolic compounds, fat and oils, reducing and non-reducing sugar and amino acids. Phytochemical screening indicated the presence of flavonoids, phenolic compounds, steroids, saponins, alkaloids, amino acids, volatile oils, reducing and hexose sugar. The phytochemical composition of the leaves of the plants indicates their medicinal properties.","PeriodicalId":14906,"journal":{"name":"Journal of Advanced Scientific Research","volume":"35 7","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-11-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139196497","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Infection with HIV results in discordant immunological response characterized by dysregualtion of cytokines and adecrease in CD4+ T cells. As HIV progresses to AIDS, there is a switch in Th1 to Th2 cytokines. In this study, plasmacytokine levels were compared between newly diagnosed HIV-positive HAART naïve patients and HAART undergoingpatients. Additionally, the association between plasma cytokine levels and CD4+ T cell count was examined. 160 HIVpositiveindividuals were enrolled for the study, of which 80 were HAART naïve and 80 were undergoing HAART forover six months since diagnosis. The plasma cytokine levels of IFN Ɣ, IL 2, IL 4 and IL 10 were analysed using ELISAand CD4+ T cell counts were assessed using flow cytometry. Comparing the HAART naïve patients to HAARTundergoing group, the plasma cytokine concentrations of IL 4 and IL 10 was considerably higher in HAART naïvepatients while IFN Ɣ and IL 2 concentration was greater in HAART undergoing patients. Similarly, while examining therelationship between CD4+ T cells and plasma cytokine levels, it was discovered that IFN Ɣ significantly correlated withCD4+ T cells in HAART-naive individuals as opposed to IL 2, IL 4, and IL 10. Therefore, an increase in Th1 cytokinesover Th2 cytokines and the restoration of immune response are indicators of successful anti-retroviral therapy
感染艾滋病毒会导致不和谐的免疫反应，其特点是细胞因子失调和 CD4+ T 细胞减少。随着艾滋病的发展，Th1 细胞因子向 Th2 细胞因子转变。本研究比较了新诊断出的 HIV 阳性 HAART 新患者和接受 HAART 治疗的患者的血浆细胞因子水平。此外，还研究了血浆细胞因子水平与 CD4+ T 细胞计数之间的关联。这项研究共招募了 160 名艾滋病病毒抗体阳性患者，其中 80 人是 HAART 新患者，80 人是确诊后接受 HAART 治疗超过 6 个月的患者。使用酶联免疫吸附法分析了血浆中 IFN Ɣ、IL 2、IL 4 和 IL 10 的细胞因子水平，并使用流式细胞术评估了 CD4+ T 细胞计数。将接受 HAART 治疗的患者与接受 HAART 治疗的患者进行比较，发现接受 HAART 治疗的患者血浆中 IL 4 和 IL 10 的细胞因子浓度要高得多，而接受 HAART 治疗的患者 IFN Ɣ 和 IL 2 的浓度要高得多。同样，在研究 CD4+ T 细胞与血浆细胞因子水平之间的关系时，发现 IFN Ɣ 与 HAART 未接受治疗者的 CD4+ T 细胞显著相关，而 IL 2、IL 4 和 IL 10 则相反。因此，Th1 细胞因子的增加超过 Th2 细胞因子的增加以及免疫反应的恢复是抗逆转录病毒治疗成功的指标。
{"title":"PREPONDERANCE EFFECT OF TH1 CYTOKINES IN HIV PATIENTS UNDERGOING HAART","authors":"Jerusha Daniel, E. Manickan","doi":"10.55218/jasr.202314905","DOIUrl":"https://doi.org/10.55218/jasr.202314905","url":null,"abstract":"Infection with HIV results in discordant immunological response characterized by dysregualtion of cytokines and adecrease in CD4+ T cells. As HIV progresses to AIDS, there is a switch in Th1 to Th2 cytokines. In this study, plasmacytokine levels were compared between newly diagnosed HIV-positive HAART naïve patients and HAART undergoingpatients. Additionally, the association between plasma cytokine levels and CD4+ T cell count was examined. 160 HIVpositiveindividuals were enrolled for the study, of which 80 were HAART naïve and 80 were undergoing HAART forover six months since diagnosis. The plasma cytokine levels of IFN Ɣ, IL 2, IL 4 and IL 10 were analysed using ELISAand CD4+ T cell counts were assessed using flow cytometry. Comparing the HAART naïve patients to HAARTundergoing group, the plasma cytokine concentrations of IL 4 and IL 10 was considerably higher in HAART naïvepatients while IFN Ɣ and IL 2 concentration was greater in HAART undergoing patients. Similarly, while examining therelationship between CD4+ T cells and plasma cytokine levels, it was discovered that IFN Ɣ significantly correlated withCD4+ T cells in HAART-naive individuals as opposed to IL 2, IL 4, and IL 10. Therefore, an increase in Th1 cytokinesover Th2 cytokines and the restoration of immune response are indicators of successful anti-retroviral therapy","PeriodicalId":14906,"journal":{"name":"Journal of Advanced Scientific Research","volume":"59 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139308144","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Tea garden soil extensively supports monoculture plantation of tea plant and there is comparatively little informationabout the distribution of spores of entomopathogenic fungi within the studied site. The entomopathogenic fungi arepromising natural agents that can parasitize a wide range of insect species and most entomopathogenic fungi have severalvariants and each strain is adapted to various hosts, causing natural infections among insects of many different species.Hence, soil samples from selected tea gardens were qualitatively analysed to find the occurrences of entomogenous fungiin the tea plantation areas which can be used as a bio-control agent against tea pests.
{"title":"ISOLATION OF POTENTIAL ENTOMOPATHOGENIC FUNGI FROM TEA SOIL OF DIBRUGARH AND TINSUKIA DISTRICT OF ASSAM","authors":"Preety Ekka, Saikia Lakhi Ram, Babu Azariah","doi":"10.55218/jasr.202314904","DOIUrl":"https://doi.org/10.55218/jasr.202314904","url":null,"abstract":"Tea garden soil extensively supports monoculture plantation of tea plant and there is comparatively little informationabout the distribution of spores of entomopathogenic fungi within the studied site. The entomopathogenic fungi arepromising natural agents that can parasitize a wide range of insect species and most entomopathogenic fungi have severalvariants and each strain is adapted to various hosts, causing natural infections among insects of many different species.Hence, soil samples from selected tea gardens were qualitatively analysed to find the occurrences of entomogenous fungiin the tea plantation areas which can be used as a bio-control agent against tea pests.","PeriodicalId":14906,"journal":{"name":"Journal of Advanced Scientific Research","volume":"22 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139309073","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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