Pub Date : 2022-12-31DOI: 10.55218/jasr.2022131108
Akshata . Sangolli, R. Nerli, S. Ghagane
Urolithiasis is a highly prevalent disease all over the world. The prevalence of kidney stone disease in India is around 12%. Fluid intake is one of the important modifiable risk factors in kidney stone disease which can be considered for preventive measures. Nevertheless, various kinds of fluids cannot have the same effect on stone formation. North Karnataka region is more prone to stone formation due to a lack of fluid intake in several parts. We aim to assess the intake of various fluids and beverages along with water in urolithiasis patients attending the urology clinic at the tertiary care centre. A total of 240 kidney stone patients attending a urology clinic at a tertiary care centre were enrolled for the study between the period of 2020 to 2021. Structured and validated questionnaires comprising detailed information about brief medical history, lifestyle habits and fluid intake habits were provided to the subjects. Kidney stones were collected from all 240 patients to analyse the chemical composition and spectral studies. Out of 240 patients, 152 were male patients and 88 were females. The overall male-to-female ratio was 1.72: 1. The mean Body Mass Index (BMI) of all the patients was 25.83. The highest numbers of cases (43.3%) were seen in the age group above 50. Around 65.8% of cases used to consume less than an adequate amount of water. In stone analysis, 58.3% were calcium oxalates and 16.6% were uric acid stones which was the second-highest among cases. Fluids like milk, tea, and fruit juices are shown to reduce the risk of stone formation to some extent whereas less water intake was a major risk factor. Alcohol and aerated beverages are still having controversial results in stone formation. So, more studies have to be conducted in different geographic regions which may help to prevent the reoccurrence and may reduce the burden of urolithiasis.
{"title":"ASSESSMENT OF FLUID INTAKE IN UROLITHIASIS PATIENTS ATTENDING UROLOGY CLINIC AT A TERTIARY CARE CENTERIN NORTH KARNATAKA","authors":"Akshata . Sangolli, R. Nerli, S. Ghagane","doi":"10.55218/jasr.2022131108","DOIUrl":"https://doi.org/10.55218/jasr.2022131108","url":null,"abstract":"Urolithiasis is a highly prevalent disease all over the world. The prevalence of kidney stone disease in India is around 12%. Fluid intake is one of the important modifiable risk factors in kidney stone disease which can be considered for preventive measures. Nevertheless, various kinds of fluids cannot have the same effect on stone formation. North Karnataka region is more prone to stone formation due to a lack of fluid intake in several parts. We aim to assess the intake of various fluids and beverages along with water in urolithiasis patients attending the urology clinic at the tertiary care centre. A total of 240 kidney stone patients attending a urology clinic at a tertiary care centre were enrolled for the study between the period of 2020 to 2021. Structured and validated questionnaires comprising detailed information about brief medical history, lifestyle habits and fluid intake habits were provided to the subjects. Kidney stones were collected from all 240 patients to analyse the chemical composition and spectral studies. Out of 240 patients, 152 were male patients and 88 were females. The overall male-to-female ratio was 1.72: 1. The mean Body Mass Index (BMI) of all the patients was 25.83. The highest numbers of cases (43.3%) were seen in the age group above 50. Around 65.8% of cases used to consume less than an adequate amount of water. In stone analysis, 58.3% were calcium oxalates and 16.6% were uric acid stones which was the second-highest among cases. Fluids like milk, tea, and fruit juices are shown to reduce the risk of stone formation to some extent whereas less water intake was a major risk factor. Alcohol and aerated beverages are still having controversial results in stone formation. So, more studies have to be conducted in different geographic regions which may help to prevent the reoccurrence and may reduce the burden of urolithiasis.","PeriodicalId":14906,"journal":{"name":"Journal of Advanced Scientific Research","volume":"70 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73502229","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-12-31DOI: 10.55218/jasr.2022131109
Jyoti Bhusan Singh, P. Mourya, G. Rai, R. Shukla
The aim of present research work was to prepare and evaluate the controlled release bilayered tablet of Nicardipine HCL to improve its bioavailability for the treatment of hypertension. To minimize critical process parameters, two layer compression method was used for the formulation of Bilayered tablets. The appropriate formulation was achieved successfully with the combination of Polymers MCC, Carbopol 71G and HPMC K100M produced desired release profile for Metoprolol succinate extended release layer. The combination of disintegrating agents that is Sodium starch glycolate and Dicalcium phosphate produced desired release rate for Nicardipine immediate release layer. The results reveal that formulation F7 has met the objective of controlled drug release for over a period of 12 hrs. The formulation F7 ascertained the efficacy of the controlled released Bilayered tablet of Nicardipine and Metoprolol ER tablet in hypertension. This sustained release Bilayered tablet with the combination of Nicardipine and metoprolol can be used in the management of different types of hypertension. The formulation F7 of combination of Nicardipine and metoprolol showed controlled release profile among the other, Hence it was considered as an optimized formulation.
{"title":"FORMULATION AND IN-VITRO EVALUATION OF NICARDIPINE HYDROCHLORIDE BILAYERED TABLET FOR CONTROLLED RELEASE","authors":"Jyoti Bhusan Singh, P. Mourya, G. Rai, R. Shukla","doi":"10.55218/jasr.2022131109","DOIUrl":"https://doi.org/10.55218/jasr.2022131109","url":null,"abstract":"The aim of present research work was to prepare and evaluate the controlled release bilayered tablet of Nicardipine HCL to improve its bioavailability for the treatment of hypertension. To minimize critical process parameters, two layer compression method was used for the formulation of Bilayered tablets. The appropriate formulation was achieved successfully with the combination of Polymers MCC, Carbopol 71G and HPMC K100M produced desired release profile for Metoprolol succinate extended release layer. The combination of disintegrating agents that is Sodium starch glycolate and Dicalcium phosphate produced desired release rate for Nicardipine immediate release layer. The results reveal that formulation F7 has met the objective of controlled drug release for over a period of 12 hrs. The formulation F7 ascertained the efficacy of the controlled released Bilayered tablet of Nicardipine and Metoprolol ER tablet in hypertension. This sustained release Bilayered tablet with the combination of Nicardipine and metoprolol can be used in the management of different types of hypertension. The formulation F7 of combination of Nicardipine and metoprolol showed controlled release profile among the other, Hence it was considered as an optimized formulation.","PeriodicalId":14906,"journal":{"name":"Journal of Advanced Scientific Research","volume":"35 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86541296","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-12-31DOI: 10.55218/jasr.2022131101
N. ., Sabah Mushtaq, G. Saji, Vinod Kumar Gupta
Biopesticides, and nano-biopesticides such as viruses, bacteria, fungi, algae, and nematodes are currently being used in a huge amount and their importance is increasing day by day because pesticides are already created a harmful effects on our health and environment. Due to the chemicals pesticides, diseases are increased in a high rate and food quality is decreased because toxicity level is increased. Biopesticides are best alternative to chemicals pesticides and best results to control pest control programs. The virulence of many biopesticides such as bacteria, fungus, and plant product were tested in laboratory and evaluated under field conditions and successfully approval by government. Biopesticide products are now sold in market for the control of pest and diseases. The aim of biopesticide research is to make these biopesticide products available at field level at a reasonable price and this would become a possible step in the integrated pest management strategy. Moreover, biopesticide research is still going on and in future more products are needed in aspects of bioformulation and areas such as commercialization. This review paper has the important and basic mode of action of major biopesticides in the past. The future prospects for the development of new biopesticides are also discussed such as nano- biopesticides.
{"title":"MODE OF ACTION OF BIOPESTICIDES AGAINST PESTS AND FUTURE PROSPECTS OF BIOPESTICIDES AND NANOBIOPESTICIDES","authors":"N. ., Sabah Mushtaq, G. Saji, Vinod Kumar Gupta","doi":"10.55218/jasr.2022131101","DOIUrl":"https://doi.org/10.55218/jasr.2022131101","url":null,"abstract":"Biopesticides, and nano-biopesticides such as viruses, bacteria, fungi, algae, and nematodes are currently being used in a huge amount and their importance is increasing day by day because pesticides are already created a harmful effects on our health and environment. Due to the chemicals pesticides, diseases are increased in a high rate and food quality is decreased because toxicity level is increased. Biopesticides are best alternative to chemicals pesticides and best results to control pest control programs. The virulence of many biopesticides such as bacteria, fungus, and plant product were tested in laboratory and evaluated under field conditions and successfully approval by government. Biopesticide products are now sold in market for the control of pest and diseases. The aim of biopesticide research is to make these biopesticide products available at field level at a reasonable price and this would become a possible step in the integrated pest management strategy. Moreover, biopesticide research is still going on and in future more products are needed in aspects of bioformulation and areas such as commercialization. This review paper has the important and basic mode of action of major biopesticides in the past. The future prospects for the development of new biopesticides are also discussed such as nano- biopesticides.","PeriodicalId":14906,"journal":{"name":"Journal of Advanced Scientific Research","volume":"7 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83367167","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-12-31DOI: 10.55218/jasr.2022131111
Shanmugam . Pandirajan, S. Samuel, V. Iswaran, Ramaswamy Venkatachalam
India’s flora and fauna has diverse medicinal properties and constitutes the major portion of our traditional medicine system. One among them is snails. Snails have a long history of being used for treating diseases in traditional medicinal systems around the world including India and China. Pila globosa is one of the largest land snails, mainly used as food. Here in this study, the gas chromatography and mass spectrometry discovered the presence of thirteen bio active constituents with various medicinal applications. The constituent Stigmasta-4, 22-dien-3-beta-ol had the highest retention time and exhibited a efficient binding with the human protein calcitonin receptor ectodomain. The calcitonin receptor types is expressed widely in various tissues and cells but most importantly in the osteoclates and hence a very important receptor in the treatment for bone disorders. The binding of the Pila globosa snail constituent with the human calcitonin receptor proved that a novel drug can be developed from the snail for the treatment of bone disorders such as osteoporosis, hypercalcemia of malignancy and paget’s disease. In addition to that, the snail extract exhibited no cytotoxicity and an efficient antioxidant property against DPPH and H2O2 free radicals. The FTIR analysis revealed the snail contained biologically significant functional groups with properties suitable for a drug and prevalent in most of the pharmaceutical drugs. Thus, these results imply the safety and efficacy of the snail Pila globosa for a novel drug development.
{"title":"GC- MS SCREENING AND IN-SILICO PREDICTION OF PILA GLOBOSA EXTRACT AGAINST THE BONE DISEASES IN CALCITONIN RECEPTOR","authors":"Shanmugam . Pandirajan, S. Samuel, V. Iswaran, Ramaswamy Venkatachalam","doi":"10.55218/jasr.2022131111","DOIUrl":"https://doi.org/10.55218/jasr.2022131111","url":null,"abstract":"India’s flora and fauna has diverse medicinal properties and constitutes the major portion of our traditional medicine system. One among them is snails. Snails have a long history of being used for treating diseases in traditional medicinal systems around the world including India and China. Pila globosa is one of the largest land snails, mainly used as food. Here in this study, the gas chromatography and mass spectrometry discovered the presence of thirteen bio active constituents with various medicinal applications. The constituent Stigmasta-4, 22-dien-3-beta-ol had the highest retention time and exhibited a efficient binding with the human protein calcitonin receptor ectodomain. The calcitonin receptor types is expressed widely in various tissues and cells but most importantly in the osteoclates and hence a very important receptor in the treatment for bone disorders. The binding of the Pila globosa snail constituent with the human calcitonin receptor proved that a novel drug can be developed from the snail for the treatment of bone disorders such as osteoporosis, hypercalcemia of malignancy and paget’s disease. In addition to that, the snail extract exhibited no cytotoxicity and an efficient antioxidant property against DPPH and H2O2 free radicals. The FTIR analysis revealed the snail contained biologically significant functional groups with properties suitable for a drug and prevalent in most of the pharmaceutical drugs. Thus, these results imply the safety and efficacy of the snail Pila globosa for a novel drug development.","PeriodicalId":14906,"journal":{"name":"Journal of Advanced Scientific Research","volume":"251 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78373447","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-12-31DOI: 10.55218/jasr.2022131110
Jissy Mathew
The fabrication of chitosan/polystyrene sulfonate (CHI)/(PSS) polyelectrolyte multilayer on different polymeric supports (polyether sulfone, polycarbonate and nylon), is discussed in the present work. The permeation of model protein BSA through these multilayer membranes was carried out under ultrafiltration conditions. Different multilayer systems were prepared by varying the parameters such as nature of the polymeric support, pH and molecular weight of polyelectrolyte. Build up of multilayers on polymeric support was monitored by measuring area under the sulfonate peak at 1033 cm-1 in the FTIR spectrum. No appreciable rejection of BSA was observed when CHI/PSS multilayers were fabricated on polycarbonate and nylon supports even after 15 bilayer coating. When multilayers were fabricated on polyether sulfone membranes, more than 90% rejection of BSA was observed with 5 bilayer coated membrane. This study reveals that pH and molecular weight of the polyelectrolyte has decisive role in the multilayer build up.
{"title":"FABRICATION OF NANO-STRUCTURED SURFACES BY FINE TUNING OF DEPOSITION PARAMETERS IN CHITOSAN/PSS POLYELECTROLYTE MULTILAYERS","authors":"Jissy Mathew","doi":"10.55218/jasr.2022131110","DOIUrl":"https://doi.org/10.55218/jasr.2022131110","url":null,"abstract":"The fabrication of chitosan/polystyrene sulfonate (CHI)/(PSS) polyelectrolyte multilayer on different polymeric supports (polyether sulfone, polycarbonate and nylon), is discussed in the present work. The permeation of model protein BSA through these multilayer membranes was carried out under ultrafiltration conditions. Different multilayer systems were prepared by varying the parameters such as nature of the polymeric support, pH and molecular weight of polyelectrolyte. Build up of multilayers on polymeric support was monitored by measuring area under the sulfonate peak at 1033 cm-1 in the FTIR spectrum. No appreciable rejection of BSA was observed when CHI/PSS multilayers were fabricated on polycarbonate and nylon supports even after 15 bilayer coating. When multilayers were fabricated on polyether sulfone membranes, more than 90% rejection of BSA was observed with 5 bilayer coated membrane. This study reveals that pH and molecular weight of the polyelectrolyte has decisive role in the multilayer build up.","PeriodicalId":14906,"journal":{"name":"Journal of Advanced Scientific Research","volume":"23 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85432986","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-12-31DOI: 10.55218/jasr.2022131107
E. M. V. Goud, Roshanara Ali, V. V. Rajesham, T. Rao
Lower respiratory infections are generally caused by viral or bacterial agents. The majority of bronchitis and bronchiolitis cases are caused by viruses. Streptococcus pneumoniae is the most common bacterial agent in community-acquired pneumonias. An open, randomized study of the efficacy and safety of the prototype antibiotics azithromycin, and erythromycin in the treatment of lower respiratory infection were compared. Azithromycin and Erythromycin doses were administered and studied on various cases, in the treatment of lower respiratory infections, azithromycin seems to be as effective as erythromycin and better tolerated with lower side effects and effective therapeutic effects.
{"title":"CASE REVIEW ON EFFECTS OF AZITHROMYCIN AND ERYTHROMYCIN ON LOWER RESPIRATORY INFECTION","authors":"E. M. V. Goud, Roshanara Ali, V. V. Rajesham, T. Rao","doi":"10.55218/jasr.2022131107","DOIUrl":"https://doi.org/10.55218/jasr.2022131107","url":null,"abstract":"Lower respiratory infections are generally caused by viral or bacterial agents. The majority of bronchitis and bronchiolitis cases are caused by viruses. Streptococcus pneumoniae is the most common bacterial agent in community-acquired pneumonias. An open, randomized study of the efficacy and safety of the prototype antibiotics azithromycin, and erythromycin in the treatment of lower respiratory infection were compared. Azithromycin and Erythromycin doses were administered and studied on various cases, in the treatment of lower respiratory infections, azithromycin seems to be as effective as erythromycin and better tolerated with lower side effects and effective therapeutic effects.","PeriodicalId":14906,"journal":{"name":"Journal of Advanced Scientific Research","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89702095","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-12-31DOI: 10.55218/jasr.2022131106
M. P. Mudagal, Arsha . Kv, Vincy Joseph, M. N, Nandadevi . ., S. Janadri, U. Sharma
Stress is the body's general reaction to any demand placed on it. However, in today's lifestyles, people often overlook stress or fail to recognize it as a serious illness condition. People are frequently confronted with challenging situations in today's environment. Excessive stress has been linked to the development of a number of disorders. If untreated, it may eventually lead to death. Various surveys conducted reports that everyone in a developing world is stressed in some or other the way. Statistics suggest that female experience more stress than male; it might be moderate or persistent, depending on the stressor. In mild cases it can be addressed by listening to music, reading books, and so on, but it cannot be remedied in cases of chronic stress. Chronic stress has been linked to the development of a wide range of significant diseases, including behavioural disorders (depression, anxiety), hypertension, heart disease, diabetes, and gastrointestinal disorders. In this article, we have discussed about some of the most prevalent major disorders that can be brought on by stress.
{"title":"A REVIEW ON SECONDARY MANIFESTATIONS OF STRESS","authors":"M. P. Mudagal, Arsha . Kv, Vincy Joseph, M. N, Nandadevi . ., S. Janadri, U. Sharma","doi":"10.55218/jasr.2022131106","DOIUrl":"https://doi.org/10.55218/jasr.2022131106","url":null,"abstract":"Stress is the body's general reaction to any demand placed on it. However, in today's lifestyles, people often overlook stress or fail to recognize it as a serious illness condition. People are frequently confronted with challenging situations in today's environment. Excessive stress has been linked to the development of a number of disorders. If untreated, it may eventually lead to death. Various surveys conducted reports that everyone in a developing world is stressed in some or other the way. Statistics suggest that female experience more stress than male; it might be moderate or persistent, depending on the stressor. In mild cases it can be addressed by listening to music, reading books, and so on, but it cannot be remedied in cases of chronic stress. Chronic stress has been linked to the development of a wide range of significant diseases, including behavioural disorders (depression, anxiety), hypertension, heart disease, diabetes, and gastrointestinal disorders. In this article, we have discussed about some of the most prevalent major disorders that can be brought on by stress.","PeriodicalId":14906,"journal":{"name":"Journal of Advanced Scientific Research","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75538549","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-12-31DOI: 10.55218/jasr.2022131103
Radami War, Surendra V, Sukanya Paul, U. Sharma, Suresh J, Manjunath Pm
Zebrafish are increasingly becoming popular as promising new model species for translational research in a variety of neurological fields. Because of their complex behaviors across all major neurobehavioral domains and strong genetic and physiological similarities to humans, zebrafish are well-suited to modelling many aspects of anxiety-related states. In this paper, we first summarized the behavioral models available in zebrafish, such as novel tank test, light/dark box test, open field test, and social preference test, and their efficacy in discovering anxiety-like indices in zebrafish, followed by highlighting the key neurotransmitter systems, such as glutamate and GABA. In addition, cortisol levels and gene expression were explored as well. Overall, this review discusses the benefits of using the zebrafish model for anxiety research and examines current research in the field.
{"title":"ZEBRAFISH AS AN EMERGING ALTERNATIVE TOOL FOR STUDYING ANXIETY DISORDERS","authors":"Radami War, Surendra V, Sukanya Paul, U. Sharma, Suresh J, Manjunath Pm","doi":"10.55218/jasr.2022131103","DOIUrl":"https://doi.org/10.55218/jasr.2022131103","url":null,"abstract":"Zebrafish are increasingly becoming popular as promising new model species for translational research in a variety of neurological fields. Because of their complex behaviors across all major neurobehavioral domains and strong genetic and physiological similarities to humans, zebrafish are well-suited to modelling many aspects of anxiety-related states. In this paper, we first summarized the behavioral models available in zebrafish, such as novel tank test, light/dark box test, open field test, and social preference test, and their efficacy in discovering anxiety-like indices in zebrafish, followed by highlighting the key neurotransmitter systems, such as glutamate and GABA. In addition, cortisol levels and gene expression were explored as well. Overall, this review discusses the benefits of using the zebrafish model for anxiety research and examines current research in the field.","PeriodicalId":14906,"journal":{"name":"Journal of Advanced Scientific Research","volume":"17 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81201586","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-12-31DOI: 10.55218/jasr.2022131102
Paidigummal Uday Kumar, P. Pavithra, Sheri Sowmya, P. Deepika, P. Priya
Hand, foot and mouth disease (HFMD) is a viral disease and mainly infects infants and young children below five years of age. It’s caused by Enterovirus, most commonly the Coxsackie virus. The viruses that cause HFMD spread through close personal contact, through the air from coughing and the faeces of an infected person. This viral infection is not indigenous to one area in particular but occurs worldwide. The symptoms mainly include fever, sore throat, feeling sick, painful, blister-like lesions on the tongue, gums and inside of the cheeks, a rash on the palms, soles and sometimes the buttocks. Molecular testing is done based on the reverse transcription polymerase chain reaction (RT-PCR). Cases of HFMD are on the rise and clinicians need to know how to make the diagnosis. The majority of patients with coxsackievirus- induced hand, foot, and mouth disease are treated usually as outpatients, but those who have CNS involvement may require admission for close monitoring. Most patients with HFMD fully recover within 7-21 days.
{"title":"ENHANCING HEALTH CARE MANAGEMENT ON HFMD","authors":"Paidigummal Uday Kumar, P. Pavithra, Sheri Sowmya, P. Deepika, P. Priya","doi":"10.55218/jasr.2022131102","DOIUrl":"https://doi.org/10.55218/jasr.2022131102","url":null,"abstract":"Hand, foot and mouth disease (HFMD) is a viral disease and mainly infects infants and young children below five years of age. It’s caused by Enterovirus, most commonly the Coxsackie virus. The viruses that cause HFMD spread through close personal contact, through the air from coughing and the faeces of an infected person. This viral infection is not indigenous to one area in particular but occurs worldwide. The symptoms mainly include fever, sore throat, feeling sick, painful, blister-like lesions on the tongue, gums and inside of the cheeks, a rash on the palms, soles and sometimes the buttocks. Molecular testing is done based on the reverse transcription polymerase chain reaction (RT-PCR). Cases of HFMD are on the rise and clinicians need to know how to make the diagnosis. The majority of patients with coxsackievirus- induced hand, foot, and mouth disease are treated usually as outpatients, but those who have CNS involvement may require admission for close monitoring. Most patients with HFMD fully recover within 7-21 days.","PeriodicalId":14906,"journal":{"name":"Journal of Advanced Scientific Research","volume":"259 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79263203","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-12-31DOI: 10.55218/jasr.2022131112
D. M., E. Siril
Plants are cherished as the chief resource for healthcare products attributed to synthesizing diverse array of bio-molecules through various metabolic alterations. Over exploitation of such resources can intimidate its existence. Developing medicinal plants into crops and the use of micropropagation as technique to mass produce high-demand biomass can make a continuous supply of natural therapeutic products. Rubia cordifolia is one such plant, so an improved, efficient, reproducible and reliable in vitro clonal propagation protocol is to be adopted for the conservation of germplasm. MS medium fortified with phytohormons viz., BA, Kin, 2-iP, TDZ, mT, IAA, IBA and NAA singly or in combinations were used for the study. After one month of inoculation, 2.5 μM of BA and TDZ (9.37) endorsed the instigation of multiple shoots through axillary bud proliferation in a short span of time. In vitro produced microshoots were subjected to ex vitro rooting by treating 2.5 mM IBA for 5 min and planting in plastic pots containing potting mixture, to produce maximum rooting (79.17%) response. It was found to be an efficient and effective mode of rooting, since it combines both hardening and rooting. Under green house condition, all the rooted plantlets showed more than 90% survival. So direct regeneration protocol from nodal explants without the intervention of callus and thus ensure production of clonally uniform plants of R. cordifolia.
由于植物通过各种代谢变化合成了多种生物分子，因此被视为保健产品的主要资源。对这些资源的过度开发会威胁到它的存在。将药用植物培育成农作物，并利用微繁技术大规模生产高需求的生物质，可以使天然治疗产品持续供应。因此，需要采用一种改良的、高效的、可复制的、可靠的离体无性系繁殖方案进行种质资源的保存。在MS培养基中分别添加植物激素:BA、Kin、2-iP、TDZ、mT、IAA、IBA和NAA。接种1个月后，2.5 μM BA和TDZ(9.37)在短时间内通过腋芽增殖诱导多芽形成。用2.5 mM IBA处理5 min后，在含盆栽混合液的塑料盆中种植，离体生根效果最好，达79.17%。它是一种高效的生根方式，因为它结合了硬化和生根。在温室条件下，所有生根植株成活率均在90%以上。因此，在不干预愈伤组织的情况下，采用节形外植体直接再生的方法，可以保证堇青花植株的无性系一致性。
{"title":"MICROPROPAGATION OF PHARMACEUTICAL CROP RUBIA CORDIFOLIA","authors":"D. M., E. Siril","doi":"10.55218/jasr.2022131112","DOIUrl":"https://doi.org/10.55218/jasr.2022131112","url":null,"abstract":"Plants are cherished as the chief resource for healthcare products attributed to synthesizing diverse array of bio-molecules through various metabolic alterations. Over exploitation of such resources can intimidate its existence. Developing medicinal plants into crops and the use of micropropagation as technique to mass produce high-demand biomass can make a continuous supply of natural therapeutic products. Rubia cordifolia is one such plant, so an improved, efficient, reproducible and reliable in vitro clonal propagation protocol is to be adopted for the conservation of germplasm. MS medium fortified with phytohormons viz., BA, Kin, 2-iP, TDZ, mT, IAA, IBA and NAA singly or in combinations were used for the study. After one month of inoculation, 2.5 μM of BA and TDZ (9.37) endorsed the instigation of multiple shoots through axillary bud proliferation in a short span of time. In vitro produced microshoots were subjected to ex vitro rooting by treating 2.5 mM IBA for 5 min and planting in plastic pots containing potting mixture, to produce maximum rooting (79.17%) response. It was found to be an efficient and effective mode of rooting, since it combines both hardening and rooting. Under green house condition, all the rooted plantlets showed more than 90% survival. So direct regeneration protocol from nodal explants without the intervention of callus and thus ensure production of clonally uniform plants of R. cordifolia.","PeriodicalId":14906,"journal":{"name":"Journal of Advanced Scientific Research","volume":"10 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79106644","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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