Pub Date : 2023-03-03DOI: 10.22358/jafs/157536/2023
I. Polovyi, S. Vovk, M. Petryshyn
. The aim of the current study was to analyse the effect of different doses of a new probiotic (produced in Ukraine from Saccharomyces cerevisiae ) in the feed on the production of nitrogen metabolites in the rumen, enzymatic activity of the digestive tract microbiota and growth rate of young ewes. The experiments showed that the supplementation of the probiotic EnzActive at doses of 0.4, 0.8 and 1.2% (w/w) to concentrate feed for 11–12 month old sheep had a positive effect on the production of nitrogen compounds, enzymatic activity of the rumen microbiota and animal growth intensity. It was proven that the introduction of the specified probiotic at a dose of 1.2% significantly increased the production of total nitrogen and amylolytic, proteolytic and cellulolytic activity of the rumen microbiota compared to animals that did not receive it. It was also found that the highest significant indices of animal growth rate during the experimental period were obtained for 0.8% probiotic supplementation
{"title":"Effect of yeast probiotic supplements in the diet of young ewes on the metabolic activity of rumen microbiota","authors":"I. Polovyi, S. Vovk, M. Petryshyn","doi":"10.22358/jafs/157536/2023","DOIUrl":"https://doi.org/10.22358/jafs/157536/2023","url":null,"abstract":". The aim of the current study was to analyse the effect of different doses of a new probiotic (produced in Ukraine from Saccharomyces cerevisiae ) in the feed on the production of nitrogen metabolites in the rumen, enzymatic activity of the digestive tract microbiota and growth rate of young ewes. The experiments showed that the supplementation of the probiotic EnzActive at doses of 0.4, 0.8 and 1.2% (w/w) to concentrate feed for 11–12 month old sheep had a positive effect on the production of nitrogen compounds, enzymatic activity of the rumen microbiota and animal growth intensity. It was proven that the introduction of the specified probiotic at a dose of 1.2% significantly increased the production of total nitrogen and amylolytic, proteolytic and cellulolytic activity of the rumen microbiota compared to animals that did not receive it. It was also found that the highest significant indices of animal growth rate during the experimental period were obtained for 0.8% probiotic supplementation","PeriodicalId":14919,"journal":{"name":"Journal of Animal and Feed Sciences","volume":" ","pages":""},"PeriodicalIF":1.0,"publicationDate":"2023-03-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44166561","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-02-20DOI: 10.22358/jafs/156098/2023
A. Dunisławska, E. Pietrzak, A. Beldowska, A. Sławinska, M. Siwek
. The intestinal microbiota plays an important role in the signalling between the gut and the liver. Intestinal bacteria produce short-chain fatty acids and other signalling molecules that affect internal communication. Changes in the composition of the gut microbiota can lead to alternations in this communication, which may ultimately lead to modifications in gene expression driven by epigenetic mechanisms. This study aimed to determine the effect of early microbiome modifications by bioactive substances delivered in ovo on changes in the relative abundance of bacteria in the intestinal contents and the level of expression and methylation of hepatic genes. On day 12 of incubation, a probiotic, a prebiotic
{"title":"Response in liver gene expression and DNA methylation\u0000to changes in the intestinal microbial profile\u0000after in ovo stimulation of chickens","authors":"A. Dunisławska, E. Pietrzak, A. Beldowska, A. Sławinska, M. Siwek","doi":"10.22358/jafs/156098/2023","DOIUrl":"https://doi.org/10.22358/jafs/156098/2023","url":null,"abstract":". The intestinal microbiota plays an important role in the signalling between the gut and the liver. Intestinal bacteria produce short-chain fatty acids and other signalling molecules that affect internal communication. Changes in the composition of the gut microbiota can lead to alternations in this communication, which may ultimately lead to modifications in gene expression driven by epigenetic mechanisms. This study aimed to determine the effect of early microbiome modifications by bioactive substances delivered in ovo on changes in the relative abundance of bacteria in the intestinal contents and the level of expression and methylation of hepatic genes. On day 12 of incubation, a probiotic, a prebiotic","PeriodicalId":14919,"journal":{"name":"Journal of Animal and Feed Sciences","volume":" ","pages":""},"PeriodicalIF":1.0,"publicationDate":"2023-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43665357","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-02-20DOI: 10.22358/jafs/159227/2023
B. Park, J. Shin, G. Son, S. Lee, J. Park, J. Jhoo, G. Kim
,
,
{"title":"Effects of full fat oil seeds on milking performance,\u0000milk composition and milk quality in lactating Holstein cows","authors":"B. Park, J. Shin, G. Son, S. Lee, J. Park, J. Jhoo, G. Kim","doi":"10.22358/jafs/159227/2023","DOIUrl":"https://doi.org/10.22358/jafs/159227/2023","url":null,"abstract":",","PeriodicalId":14919,"journal":{"name":"Journal of Animal and Feed Sciences","volume":" ","pages":""},"PeriodicalIF":1.0,"publicationDate":"2023-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46198693","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-02-10DOI: 10.22358/jafs/157543/2023
S. Park, V. Zammit
. Digital livestock system through convergence of livestock production and information and communication technology is being applied to livestock farms to improve animal behavior and welfare, production, and quality of animal food. In previously study, we noted that the egg production were greatly enhanced in laying hens using digital livestock system. The present study investigated effects of a digital livestock system on fatty acid profiles and cholesterol of eggs, animal behavior, and welfare of laying hens. A total of 300 laying hens (Hy-Line Brown) at 48 weeks old were divided into two treatment groups: conventional livestock system (CON) and digital livestock system (DLS) in a randomized complete block design for 10 weeks. Drinking, feather squatting, eating, moving, preening, and resting scores as behavior indicators of laying hens were significantly improved in the DLS group than in the CON group (all P < 0.05). Animal welfare scores such as appearance, feather condition, body condition, and health of laying hens were significantly higher in the DLS group than in the CON group ( P < 0.05). Contents of oleic acid and unsaturated fatty acid of eggs were significantly increased in the DLS group compared to the CON group ( P < 0.05). However, content of saturated fatty acid and n-6/n-3 fatty acid ratio of eggs of the DLS group were significantly lower than those in the CON group ( P < 0.05). These results indicate that the digital livestock system can be used as a future livestock farming algorithm to significantly improve egg fatty acid profile, animal behavior, and welfare in laying hens.
{"title":"Effect of digital livestock system on animal behavior and welfare,and fatty acid profiles of egg in laying hens","authors":"S. Park, V. Zammit","doi":"10.22358/jafs/157543/2023","DOIUrl":"https://doi.org/10.22358/jafs/157543/2023","url":null,"abstract":". Digital livestock system through convergence of livestock production and information and communication technology is being applied to livestock farms to improve animal behavior and welfare, production, and quality of animal food. In previously study, we noted that the egg production were greatly enhanced in laying hens using digital livestock system. The present study investigated effects of a digital livestock system on fatty acid profiles and cholesterol of eggs, animal behavior, and welfare of laying hens. A total of 300 laying hens (Hy-Line Brown) at 48 weeks old were divided into two treatment groups: conventional livestock system (CON) and digital livestock system (DLS) in a randomized complete block design for 10 weeks. Drinking, feather squatting, eating, moving, preening, and resting scores as behavior indicators of laying hens were significantly improved in the DLS group than in the CON group (all P < 0.05). Animal welfare scores such as appearance, feather condition, body condition, and health of laying hens were significantly higher in the DLS group than in the CON group ( P < 0.05). Contents of oleic acid and unsaturated fatty acid of eggs were significantly increased in the DLS group compared to the CON group ( P < 0.05). However, content of saturated fatty acid and n-6/n-3 fatty acid ratio of eggs of the DLS group were significantly lower than those in the CON group ( P < 0.05). These results indicate that the digital livestock system can be used as a future livestock farming algorithm to significantly improve egg fatty acid profile, animal behavior, and welfare in laying hens.","PeriodicalId":14919,"journal":{"name":"Journal of Animal and Feed Sciences","volume":" ","pages":""},"PeriodicalIF":1.0,"publicationDate":"2023-02-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46477228","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-02-10DOI: 10.22358/jafs/157534/2023
M. Krauze, P. Jurczak, M. Cendrowska-Pinkosz, A. Stępniowska, P. Matusevicius, K. Ognik
. It was assumed that the addition of a phytobiotic preparation containing cinnamon oil and citric acid to water, at a properly selected dose and time of application, could reduce the occurrence of metabolic disorders of the nervous tissue leading to neurodegenerative lesions. The aim of the study was to compare the effect of selected doses of the phytobiotic administered over different periods of time on the level of
{"title":"Feasibility of including a phytobiotic containing cinnamon oil\u0000in the diet to reduce the occurrence of neurodegenerative\u0000changes in broiler chicken tissues","authors":"M. Krauze, P. Jurczak, M. Cendrowska-Pinkosz, A. Stępniowska, P. Matusevicius, K. Ognik","doi":"10.22358/jafs/157534/2023","DOIUrl":"https://doi.org/10.22358/jafs/157534/2023","url":null,"abstract":". It was assumed that the addition of a phytobiotic preparation containing cinnamon oil and citric acid to water, at a properly selected dose and time of application, could reduce the occurrence of metabolic disorders of the nervous tissue leading to neurodegenerative lesions. The aim of the study was to compare the effect of selected doses of the phytobiotic administered over different periods of time on the level of","PeriodicalId":14919,"journal":{"name":"Journal of Animal and Feed Sciences","volume":" ","pages":""},"PeriodicalIF":1.0,"publicationDate":"2023-02-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47071018","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-02-09DOI: 10.22358/jafs/157501/2023
V. Pirgozliev, I. Whiting, S. Mansbridge, J. Abdullah, W. Mirza, S. Rose
. Concerns over the sustainability of using soybean in poultry diets, has steered the need to develop alternative protein sources for modern poultry production. This experiment examined the effect of nine different United Kingdom grown field bean ( Vicia faba L. var. minor ) cultivars from the same harvest year on apparent metabolizable energy (AME) and dry matter digestibility (DMD) when fed to Hy-Line Brown pullets from 14 to 16 weeks of age. A balancer feed was formulated to contain 11.54 MJ/kg AME and 166.4 g/kg crude protein. Nine nutritionally complete, meal-form diets were then prepared by mixing 200 g/kg of each field bean cultivar with 800 g/kg of balancer feed, making a total of ten diets. Each diet was fed to eight cages containing two birds. AME was determined via total collection technique over the last four days of the study. Relationships were examined between AME and the chemical composition of the beans. The overall determined AME value of the beans ranged from 9.31 MJ/kg DM to 12.26 MJ/kg DM, giving an average AME of 10.94 MJ/kg DM ( P < 0.001). Total non-starch polysaccharide (NSP) content of the beans negatively correlated with their determined AME ( P < 0.05) and also tended ( P = 0.066) to negatively reduce AME based on regression analysis. There is evidence that total NSP may be used by nutritionists to predict AME value of field beans, however, stronger evidence is required. This information may be used by plant breeders to aid in the development of new field bean cultivars.
{"title":"Feeding value of field bean (Vicia faba L. var. minor)\u0000for laying hen pullets","authors":"V. Pirgozliev, I. Whiting, S. Mansbridge, J. Abdullah, W. Mirza, S. Rose","doi":"10.22358/jafs/157501/2023","DOIUrl":"https://doi.org/10.22358/jafs/157501/2023","url":null,"abstract":". Concerns over the sustainability of using soybean in poultry diets, has steered the need to develop alternative protein sources for modern poultry production. This experiment examined the effect of nine different United Kingdom grown field bean ( Vicia faba L. var. minor ) cultivars from the same harvest year on apparent metabolizable energy (AME) and dry matter digestibility (DMD) when fed to Hy-Line Brown pullets from 14 to 16 weeks of age. A balancer feed was formulated to contain 11.54 MJ/kg AME and 166.4 g/kg crude protein. Nine nutritionally complete, meal-form diets were then prepared by mixing 200 g/kg of each field bean cultivar with 800 g/kg of balancer feed, making a total of ten diets. Each diet was fed to eight cages containing two birds. AME was determined via total collection technique over the last four days of the study. Relationships were examined between AME and the chemical composition of the beans. The overall determined AME value of the beans ranged from 9.31 MJ/kg DM to 12.26 MJ/kg DM, giving an average AME of 10.94 MJ/kg DM ( P < 0.001). Total non-starch polysaccharide (NSP) content of the beans negatively correlated with their determined AME ( P < 0.05) and also tended ( P = 0.066) to negatively reduce AME based on regression analysis. There is evidence that total NSP may be used by nutritionists to predict AME value of field beans, however, stronger evidence is required. This information may be used by plant breeders to aid in the development of new field bean cultivars.","PeriodicalId":14919,"journal":{"name":"Journal of Animal and Feed Sciences","volume":" ","pages":""},"PeriodicalIF":1.0,"publicationDate":"2023-02-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47260127","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-02-03DOI: 10.22358/jafs/157475/2023
M.Y. Liu, L. Wu, X. Lv, L. He, J. Hao, B. Ma, L. Xi, Y. Qiao, F. Tang, J. Han
. This study evaluated the effect of 25-hydroxycholecalciferol (25-hydroxyvitamin D 3
本研究评估了25-羟基胆钙化醇（25-羟基维生素D3
{"title":"25-hydroxycholecalciferol affects growth performance,\u0000bone calcium content and intestinal calcium transporter\u0000gene expression in broiler chickens","authors":"M.Y. Liu, L. Wu, X. Lv, L. He, J. Hao, B. Ma, L. Xi, Y. Qiao, F. Tang, J. Han","doi":"10.22358/jafs/157475/2023","DOIUrl":"https://doi.org/10.22358/jafs/157475/2023","url":null,"abstract":". This study evaluated the effect of 25-hydroxycholecalciferol (25-hydroxyvitamin D 3","PeriodicalId":14919,"journal":{"name":"Journal of Animal and Feed Sciences","volume":" ","pages":""},"PeriodicalIF":1.0,"publicationDate":"2023-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49277594","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-01-31DOI: 10.22358/jafs/157555/2023
T. Wahyono, R. Wahyuningsih, A. Setiyawan, D. Pratiwi, T. Kurniawan, S. Hariyadi, Mohammad Miftakhus Sholikin, A. Jayanegara, E. Triyannanto, A. Febrisiantosa
. The objective of this study was to evaluate the effects of dietary selenium supplementation (organic and inorganic) on carcass characteristics and meat quality in ruminants. The data
本研究的目的是评估日粮补硒（有机和无机）对反刍动物胴体特性和肉质的影响。数据
{"title":"Effect of dietary selenium supplementation\u0000(organic and inorganic) on carcass characteristics\u0000and meat quality of ruminants: a meta-analysis","authors":"T. Wahyono, R. Wahyuningsih, A. Setiyawan, D. Pratiwi, T. Kurniawan, S. Hariyadi, Mohammad Miftakhus Sholikin, A. Jayanegara, E. Triyannanto, A. Febrisiantosa","doi":"10.22358/jafs/157555/2023","DOIUrl":"https://doi.org/10.22358/jafs/157555/2023","url":null,"abstract":". The objective of this study was to evaluate the effects of dietary selenium supplementation (organic and inorganic) on carcass characteristics and meat quality in ruminants. The data","PeriodicalId":14919,"journal":{"name":"Journal of Animal and Feed Sciences","volume":" ","pages":""},"PeriodicalIF":1.0,"publicationDate":"2023-01-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41530067","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-01-27DOI: 10.22358/jafs/157531/2023
S. Kamiński, T. Zabolewicz, K. Oleński, A. Babuchowski
. The purpose of the study was to analyse changes in the frequency of genotypes and alleles of beta-casein ( CASB ), kappa-casein ( CSN3 ) and beta-lactoglobulin ( LGB ) loci among bulls (used for insemination in 1991–2021) and cows (milked in 2014 and 2021). The analysis included 1812 bulls and 2628 cows of the Polish Holstein-Friesian breed. Genotypes of three milk protein loci were identified using PCR-RFLP/ACRS method (bulls) or the Illumina Bovine MDv2 Chip (cows). For bulls used in insemination between 1991 and 2021, the frequency of the desirable CSN3 allele B and allele A2 at the CASB locus increased by 44% and 8%, respectively. In cows, the frequency of allele B of CSN3 and allele A2 of CASB increased over the past seven years by 11% and 7%, respectively. The frequency of LGB allele B decreased in cows from 47% to 41%. An increase in the frequency of the rarest allele E at the CSN3 locus in bulls was also observed (from 2% to 7%). The number of animals carrying the most desirable combination of CASB and CSN3 genotypes (A2A2 and BB) reached 4.25% and 2.8% in cows and bulls, respectively. The current results showed a slow but marked increase in the desirable alleles at casein gene loci which should improve milk quality and cheese-processing properties. This positive effect was attenuated by the simultaneous decrease in the frequency of LGB allele B.
{"title":"Long-term changes in the frequency of beta-casein,\u0000kappa-casein and beta-lactoglobulin alleles\u0000in Polish Holstein-Friesian dairy cattle","authors":"S. Kamiński, T. Zabolewicz, K. Oleński, A. Babuchowski","doi":"10.22358/jafs/157531/2023","DOIUrl":"https://doi.org/10.22358/jafs/157531/2023","url":null,"abstract":". The purpose of the study was to analyse changes in the frequency of genotypes and alleles of beta-casein ( CASB ), kappa-casein ( CSN3 ) and beta-lactoglobulin ( LGB ) loci among bulls (used for insemination in 1991–2021) and cows (milked in 2014 and 2021). The analysis included 1812 bulls and 2628 cows of the Polish Holstein-Friesian breed. Genotypes of three milk protein loci were identified using PCR-RFLP/ACRS method (bulls) or the Illumina Bovine MDv2 Chip (cows). For bulls used in insemination between 1991 and 2021, the frequency of the desirable CSN3 allele B and allele A2 at the CASB locus increased by 44% and 8%, respectively. In cows, the frequency of allele B of CSN3 and allele A2 of CASB increased over the past seven years by 11% and 7%, respectively. The frequency of LGB allele B decreased in cows from 47% to 41%. An increase in the frequency of the rarest allele E at the CSN3 locus in bulls was also observed (from 2% to 7%). The number of animals carrying the most desirable combination of CASB and CSN3 genotypes (A2A2 and BB) reached 4.25% and 2.8% in cows and bulls, respectively. The current results showed a slow but marked increase in the desirable alleles at casein gene loci which should improve milk quality and cheese-processing properties. This positive effect was attenuated by the simultaneous decrease in the frequency of LGB allele B.","PeriodicalId":14919,"journal":{"name":"Journal of Animal and Feed Sciences","volume":" ","pages":""},"PeriodicalIF":1.0,"publicationDate":"2023-01-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48372025","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-12-20DOI: 10.22358/jafs/156762/2022
A. Szczepkowska, J. Bochenek, M. Wójcik, D. Tomaszewska-Zaremba, H. Antushevich, M. Tomczyk, J. Skipor, A. Herman
. Caffeine is a plant alkaloid that stimulates the central nervous system. It easily crosses the blood-brain barrier located in the endothelial cells of brain microvessels and the blood-cerebrospinal fluid barrier located in the epithelial cells of the choroid plexus (ChP). Caffeine exerts most of its biological effects by antagonising adenosine receptors (ADORs), but is also an agonist of ryanodine receptors (RYRs). A recent study in a sheep model has suggested that the effect of caffeine on the expression of many genes may depend on the animal’s immune status. Therefore, the aim of the study was to determine the effect of caffeine administration (iv, 30 mg/kg) in ewes, under basal and acute inflammatory conditions induced by lipopolysaccharide injection (iv, 400 ng/kg), on the expression of ADOR and RYR genes in the mediobasal hypothalamus (MBH), anterior pituitary (AP), and ChP. Our study showed that among caffeine-interacting receptors, ADORA1 was the most highly expressed in the AP and ChP, while ADORA3 in the MBH. Caffeine reduced ( P < 0.05) the inhibitory effect of inflammation on ADORA1 , but only in the MBH, and decreased ( P < 0.05) the stimulatory effect of endotoxin treatment on ADORA2B in the MBH and ChP. In contrast to ADORs , the expression of RYRs was less sensitive to the effects of inflammation and caffeine. We showed that caffeine influenced the expression of its receptor genes in the brain, but this effect seemed to be tissue-dependent and could be affected by the immune status of the animals. However, the physiological implications of these results require further detailed studies
{"title":"Effect of caffeine on adenosine and ryanodine receptor\u0000gene expression in the hypothalamus, pituitary, and choroid\u0000plexus in ewes under basal and LPS challenge conditions","authors":"A. Szczepkowska, J. Bochenek, M. Wójcik, D. Tomaszewska-Zaremba, H. Antushevich, M. Tomczyk, J. Skipor, A. Herman","doi":"10.22358/jafs/156762/2022","DOIUrl":"https://doi.org/10.22358/jafs/156762/2022","url":null,"abstract":". Caffeine is a plant alkaloid that stimulates the central nervous system. It easily crosses the blood-brain barrier located in the endothelial cells of brain microvessels and the blood-cerebrospinal fluid barrier located in the epithelial cells of the choroid plexus (ChP). Caffeine exerts most of its biological effects by antagonising adenosine receptors (ADORs), but is also an agonist of ryanodine receptors (RYRs). A recent study in a sheep model has suggested that the effect of caffeine on the expression of many genes may depend on the animal’s immune status. Therefore, the aim of the study was to determine the effect of caffeine administration (iv, 30 mg/kg) in ewes, under basal and acute inflammatory conditions induced by lipopolysaccharide injection (iv, 400 ng/kg), on the expression of ADOR and RYR genes in the mediobasal hypothalamus (MBH), anterior pituitary (AP), and ChP. Our study showed that among caffeine-interacting receptors, ADORA1 was the most highly expressed in the AP and ChP, while ADORA3 in the MBH. Caffeine reduced ( P < 0.05) the inhibitory effect of inflammation on ADORA1 , but only in the MBH, and decreased ( P < 0.05) the stimulatory effect of endotoxin treatment on ADORA2B in the MBH and ChP. In contrast to ADORs , the expression of RYRs was less sensitive to the effects of inflammation and caffeine. We showed that caffeine influenced the expression of its receptor genes in the brain, but this effect seemed to be tissue-dependent and could be affected by the immune status of the animals. However, the physiological implications of these results require further detailed studies","PeriodicalId":14919,"journal":{"name":"Journal of Animal and Feed Sciences","volume":" ","pages":""},"PeriodicalIF":1.0,"publicationDate":"2022-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42988213","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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