Pub Date : 2023-04-25DOI: 10.22358/jafs/159458/2023
J. Zhang, Y. Liu, L. Bu, Q. Liu, C. Pei, G. Guo
. The experiment was designed to evaluate the effect of sodium selenite (Na 2 SeO 3 ) on the proliferation of bovine mammary gland epithelial cells (BMECs), and to assess the underlying mechanisms. The addition of Na 2 SeO 3 at doses ranging from 18 to 140 nM stimulated BMEC proliferation. Nevertheless, high doses of Na 2 SeO 3 (> 70 nM) did not further stimulate BMEC proliferation compared to 35 nM Na 2 SeO 3 . Therefore, the addition of 35 nM Na 2 SeO 3 increased the proportion of epithelial cells undergoing DNA replication, mRNA expression of proliferating cell nuclear antigen (PCNA), Cyclin A2
{"title":"Sodium selenite addition promotes the proliferation of bovine\u0000mammary epithelial cells through the Akt-mTOR\u0000signalling pathway","authors":"J. Zhang, Y. Liu, L. Bu, Q. Liu, C. Pei, G. Guo","doi":"10.22358/jafs/159458/2023","DOIUrl":"https://doi.org/10.22358/jafs/159458/2023","url":null,"abstract":". The experiment was designed to evaluate the effect of sodium selenite (Na 2 SeO 3 ) on the proliferation of bovine mammary gland epithelial cells (BMECs), and to assess the underlying mechanisms. The addition of Na 2 SeO 3 at doses ranging from 18 to 140 nM stimulated BMEC proliferation. Nevertheless, high doses of Na 2 SeO 3 (> 70 nM) did not further stimulate BMEC proliferation compared to 35 nM Na 2 SeO 3 . Therefore, the addition of 35 nM Na 2 SeO 3 increased the proportion of epithelial cells undergoing DNA replication, mRNA expression of proliferating cell nuclear antigen (PCNA), Cyclin A2","PeriodicalId":14919,"journal":{"name":"Journal of Animal and Feed Sciences","volume":" ","pages":""},"PeriodicalIF":1.0,"publicationDate":"2023-04-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46944098","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}