Pub Date : 2023-06-25DOI: 10.22358/jafs/166080/2023
C. Hanim, N. D. Dono, B. Ariyadi, M. F. Habibi, M. Al Anas, M. F. Hanif
. Protected sodium butyrate (PSB) is considered an alternative to antibiotics in broiler diets. The present study investigated the effect of PSB supplementation on growth performance, carcass traits, relative weight of internal organs and intestinal morphology in broilers. A total of 1200 one-day-old New Lohman male broilers were randomly allocated to two dietary treatment groups (6 replicates, 100 birds per treatment) and fed a basal diet (CON) or a diet supplemented with 1 g of PSB per kg of diet for 5 weeks of the rearing period. On days 21 and 35, one bird per replicate was randomly selected and slaughtered to determine carcass traits, relative weight of internal organs, and measurements of intestinal histomorphological parameters. The results showed that dietary treatment with PSB increased body weight and average daily gain, but it also led to increased mortality rate ( P < 0.05) on day 35 compared to the control group. Furthermore, PSB supplementation increased liver weight, jejunum length and colon length, while decreasing heart weight ( P < 0.05) on day 21. In the PSB group, there was a decrease in the number of crypts in the jejunum and an increase in the height of the villi in the ileum ( P < 0.05). In conclusion, on day 21, PSB supplementation resulted in an increase in broiler digestive organ parameters, especially liver weight and intestinal morphology. Furthermore, improved growth performance of broilers was recorded on day 35.
． 保护性丁酸钠(PSB)被认为是肉仔鸡饲粮中抗生素的替代品。本试验旨在研究饲粮中添加PSB对肉仔鸡生长性能、胴体性状、内脏相对重和肠道形态的影响。试验选用1200只1日龄新洛曼肉鸡雄性，随机分为2个饲粮处理组(6个重复，每个处理100只)，饲养5周，分别饲喂基础饲粮(CON)和每kg饲粮中添加1 g PSB的饲粮。在第21天和第35天，每个重复随机选择1只鸡屠宰，测定胴体性状、内脏相对重量和肠道组织形态学参数。结果表明:饲粮中添加PSB可提高仔猪体重和平均日增重，但导致仔猪第35天的死亡率高于对照组(P < 0.05)。此外，在第21天，添加PSB增加了肝脏重量、空肠长度和结肠长度，降低了心脏重量(P < 0.05)。PSB组空肠隐窝数量减少，回肠绒毛高度增加(P < 0.05)。综上所述，在试验第21天，添加PSB增加了肉鸡消化器官参数，尤其是肝脏重量和肠道形态。此外，第35天肉鸡的生长性能有所改善。
{"title":"Effect of protected sodium butyrate on growth performance,\u0000carcass traits, relative weight of digestive organs\u0000and intestinal histomorphology of broilers","authors":"C. Hanim, N. D. Dono, B. Ariyadi, M. F. Habibi, M. Al Anas, M. F. Hanif","doi":"10.22358/jafs/166080/2023","DOIUrl":"https://doi.org/10.22358/jafs/166080/2023","url":null,"abstract":". Protected sodium butyrate (PSB) is considered an alternative to antibiotics in broiler diets. The present study investigated the effect of PSB supplementation on growth performance, carcass traits, relative weight of internal organs and intestinal morphology in broilers. A total of 1200 one-day-old New Lohman male broilers were randomly allocated to two dietary treatment groups (6 replicates, 100 birds per treatment) and fed a basal diet (CON) or a diet supplemented with 1 g of PSB per kg of diet for 5 weeks of the rearing period. On days 21 and 35, one bird per replicate was randomly selected and slaughtered to determine carcass traits, relative weight of internal organs, and measurements of intestinal histomorphological parameters. The results showed that dietary treatment with PSB increased body weight and average daily gain, but it also led to increased mortality rate ( P < 0.05) on day 35 compared to the control group. Furthermore, PSB supplementation increased liver weight, jejunum length and colon length, while decreasing heart weight ( P < 0.05) on day 21. In the PSB group, there was a decrease in the number of crypts in the jejunum and an increase in the height of the villi in the ileum ( P < 0.05). In conclusion, on day 21, PSB supplementation resulted in an increase in broiler digestive organ parameters, especially liver weight and intestinal morphology. Furthermore, improved growth performance of broilers was recorded on day 35.","PeriodicalId":14919,"journal":{"name":"Journal of Animal and Feed Sciences","volume":" ","pages":""},"PeriodicalIF":1.0,"publicationDate":"2023-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48052641","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-06-22DOI: 10.22358/jafs/163179/2023
A. Wypych, A. Dunisławska, M. Grabowska, K. Michałek, M. Ożgo, K. Liput, A. Herosimczyk, E. Polawska, M. Pierzchała, A. Lepczynski
. The Western diet, which is typically high in saturated fatty acids (SFAs) and low in n-3 polyunsaturated fatty acids (PUFAs), has been identified as a factor contributing to the growing obesity rate. Long-term consumption of high-fat diets (HFDs) has also been associated with increased risk of chronic kidney disease. Therefore, we hypothesized that different fatty acids composition in HFDs would differentially affect renal microstructure and expression pattern of selected genes. Swiss-Webster male mice (n = 24) were fed a standard chow for mice (STD
{"title":"Effects of three-month feeding high-fat diets with different\u0000fatty acid composition on kidney histology and expression\u0000of genes related to cellular stress and water-electrolyte\u0000homeostasis in mice","authors":"A. Wypych, A. Dunisławska, M. Grabowska, K. Michałek, M. Ożgo, K. Liput, A. Herosimczyk, E. Polawska, M. Pierzchała, A. Lepczynski","doi":"10.22358/jafs/163179/2023","DOIUrl":"https://doi.org/10.22358/jafs/163179/2023","url":null,"abstract":". The Western diet, which is typically high in saturated fatty acids (SFAs) and low in n-3 polyunsaturated fatty acids (PUFAs), has been identified as a factor contributing to the growing obesity rate. Long-term consumption of high-fat diets (HFDs) has also been associated with increased risk of chronic kidney disease. Therefore, we hypothesized that different fatty acids composition in HFDs would differentially affect renal microstructure and expression pattern of selected genes. Swiss-Webster male mice (n = 24) were fed a standard chow for mice (STD","PeriodicalId":14919,"journal":{"name":"Journal of Animal and Feed Sciences","volume":" ","pages":""},"PeriodicalIF":1.0,"publicationDate":"2023-06-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43067197","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-06-20DOI: 10.22358/jafs/162646/2023
N. Venkata Krishna, M. Mutha Rao, K. Veerabramhaiah, R. S. Suresh Kumar, N. S. Raghavendra, D. Yasaswini
. The current study evaluated the influence of follicle-stimulating hormone (FSH) administration on the stimulation of ovarian follicles, oocyte quality, and embryo production rate in Ongole cows with synchronized follicular wave emergence. Follicular waves were synchronized using 10 μg of gonadotropin-releasing hormone (GnRH), followed by FSH administration (250 μg) in three divided doses of 125, 75, and 50 μg at 48, 60, and 72 h post-GnRH administration, respectively. Although the total number of follicles was similar in both groups, the hormonal stimulation regimen increased ( P < 0.01) the number of large follicles without affecting small and medium follicles. FSH stimulation had no effect on the mean count of aspirated follicles; however, the cows administered FSH showed a higher ( P < 0.001) aspiration rate. An analysis of the proportion of cumulus oocyte complexes revealed that FSH stimulation did not affect their mean number. Nevertheless, FSH-stimulated cows had a lower recovery rate ( P < 0.001) of cumulus oocyte complexes (COCs) compared to the control group. In addition, the proportion of good-quality COCs was higher ( P < 0.01), with a simultaneous lower ( P < 0.01) percentage of poor-quality COCs in the FSH group. In vitro fertilization of both good and poor quality COCs revealed a higher ( P < 0.01) blastocyst formation rate within 7 to 8 days of fertilization. The study concluded that the FSH super-stimulation protocol improved follicular number, aspiration rate, oocyte competence and blastocyst percentage in cows synchronized for follicular wave emergence.
{"title":"Effect of FSH stimulation prior to ovum pick-up on follicular\u0000dynamics, oocyte competence, and in vitro embryo production\u0000in Ongole cows (Bos indicus)","authors":"N. Venkata Krishna, M. Mutha Rao, K. Veerabramhaiah, R. S. Suresh Kumar, N. S. Raghavendra, D. Yasaswini","doi":"10.22358/jafs/162646/2023","DOIUrl":"https://doi.org/10.22358/jafs/162646/2023","url":null,"abstract":". The current study evaluated the influence of follicle-stimulating hormone (FSH) administration on the stimulation of ovarian follicles, oocyte quality, and embryo production rate in Ongole cows with synchronized follicular wave emergence. Follicular waves were synchronized using 10 μg of gonadotropin-releasing hormone (GnRH), followed by FSH administration (250 μg) in three divided doses of 125, 75, and 50 μg at 48, 60, and 72 h post-GnRH administration, respectively. Although the total number of follicles was similar in both groups, the hormonal stimulation regimen increased ( P < 0.01) the number of large follicles without affecting small and medium follicles. FSH stimulation had no effect on the mean count of aspirated follicles; however, the cows administered FSH showed a higher ( P < 0.001) aspiration rate. An analysis of the proportion of cumulus oocyte complexes revealed that FSH stimulation did not affect their mean number. Nevertheless, FSH-stimulated cows had a lower recovery rate ( P < 0.001) of cumulus oocyte complexes (COCs) compared to the control group. In addition, the proportion of good-quality COCs was higher ( P < 0.01), with a simultaneous lower ( P < 0.01) percentage of poor-quality COCs in the FSH group. In vitro fertilization of both good and poor quality COCs revealed a higher ( P < 0.01) blastocyst formation rate within 7 to 8 days of fertilization. The study concluded that the FSH super-stimulation protocol improved follicular number, aspiration rate, oocyte competence and blastocyst percentage in cows synchronized for follicular wave emergence.","PeriodicalId":14919,"journal":{"name":"Journal of Animal and Feed Sciences","volume":" ","pages":""},"PeriodicalIF":1.0,"publicationDate":"2023-06-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43038720","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-06-15DOI: 10.22358/jafs/161778/2023
J. Zhao, H. Liu, X. Yin, J. Li, Z. Dong, S. Wang, T. Shao
. With the increasing shortage of feed resources, hybrid Pennisetum (HE) shows promise as a potential animal feedstuff. The present study aimed to evaluate the fermentation quality, microbial community and functional shifts in HE during ensiling. HE was ensiled naturally (NHE) for 1, 3, 7,
{"title":"Relationships between microbial community, functional profile\u0000and fermentation quality during ensiling of hybrid Pennisetum","authors":"J. Zhao, H. Liu, X. Yin, J. Li, Z. Dong, S. Wang, T. Shao","doi":"10.22358/jafs/161778/2023","DOIUrl":"https://doi.org/10.22358/jafs/161778/2023","url":null,"abstract":". With the increasing shortage of feed resources, hybrid Pennisetum (HE) shows promise as a potential animal feedstuff. The present study aimed to evaluate the fermentation quality, microbial community and functional shifts in HE during ensiling. HE was ensiled naturally (NHE) for 1, 3, 7,","PeriodicalId":14919,"journal":{"name":"Journal of Animal and Feed Sciences","volume":" ","pages":""},"PeriodicalIF":1.0,"publicationDate":"2023-06-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43949564","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-06-13DOI: 10.22358/jafs/163632/2023
M. Czauderna, M. Karpińska, J. Woliński, K. Zaworski, M. Białek, S. Pierzynowski, Wiktoria Wojtak, K. Pierzynowska
. The objectives of our study were to enhance and evaluate an improved saponification method for the quantification of fatty acids (FA), with a specific focus on highly-unsaturated long-chain polyunsaturated FA (LPUFA) that are easily peroxidised in pig erythrocytes. These erythrocytes serve as a valuable large animal model for human
{"title":"Improved lipid saponification for chromatographic quantification\u0000of fatty acids in porcine erythrocytes – an important lipidomic\u0000biomarker of the effectiveness of dietary fat supplementation\u0000in pigs as a large animal model for human studies","authors":"M. Czauderna, M. Karpińska, J. Woliński, K. Zaworski, M. Białek, S. Pierzynowski, Wiktoria Wojtak, K. Pierzynowska","doi":"10.22358/jafs/163632/2023","DOIUrl":"https://doi.org/10.22358/jafs/163632/2023","url":null,"abstract":". The objectives of our study were to enhance and evaluate an improved saponification method for the quantification of fatty acids (FA), with a specific focus on highly-unsaturated long-chain polyunsaturated FA (LPUFA) that are easily peroxidised in pig erythrocytes. These erythrocytes serve as a valuable large animal model for human","PeriodicalId":14919,"journal":{"name":"Journal of Animal and Feed Sciences","volume":"1 1","pages":""},"PeriodicalIF":1.0,"publicationDate":"2023-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41359477","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-05-25DOI: 10.22358/jafs/162617/2023
S. Łapiński, S. Pałka, A. Otwinowska-Mindur
The long-tailed chinchilla (Chinchilla lanigera Bennett, 1829) is native to northern Chile and can be found along the foothills of the Andes and the coastal mountains south to Talca, at an elevation of 400–1650 m. Human activities, such as poaching, hunting, grazing of cattle and goats, mining, firewood extraction, and the pet trade, have posed a significant threat to this species for years. Although legislation to protect the species has been in place since 1929, it was not efficiently enforced until the establishment of the Reserva Nacional Las Chinchillas in Auco, Chile in 1983. This species has been included in the CITES Appendix I since 1977. The current wild population is estimated at around 5350 mature individuals, and despite current protection measures, populations continue to decline, and the species is classified as endangered according to the Red List Category & Criteria (Jimenez, 1996; Roach and Kennerley, 2016). Currently, chinchillas are a popular pet and farm animal, but due to ABSTRACT. The purpose of the study was to verify whether increasing the content of tyrosine and phenylalanine in the feed would result in more intense colouration of chinchilla fur and affect the animals’ reactions to behavioural tests. Chinchillas were divided into 3 groups: G-1 fed complete commercial fodder (Tyr 6.91 mg/g, Phe 7.45 mg/g), G-2 fed commercial and experimental fodder (1:1 ratio), and G-3 fed with experimental fodder (Tyr 14.31 mg/g, Phe 21.65 mg/g). During the experiment, the fur colouration type was assessed using two methods: objective, with a colorimeter (CIE L*a*b* colour space: L* – light-dark axis, a* – red-green axis, b* – yellow-blue axis, C* – colour saturation), and subjective, by a qualified chinchilla judge. A behavioural hand test was used to categorise the chinchillas’ responses to human intrusion into their cage. The results from the colorimeter measurements did not show any significant differences in the L* (P > 0.05) component; however, significant differences were observed for the a* (P = 0.0029), b* (P = 0.0218), and C* (P = 0.0342) components in relation to the fur colouration of individual groups. The assessment of the colour type by a qualified specialist found statistically significant differences (P < 0.05) between the initial and final measurements. Spearman’s correlation coefficients between colour type assessment and colour components were mostly low or medium, but with stronger values observed for L* compared to a*, b* or C*. There were no significant effects of the feeding group on the results of the and test. However, a decreasing trend was observed in the hand test results for G-3 from week 5 of the measurements. Received: 7 September 2022 Revised: 23 March 2022 Accepted: 25 March 2022
{"title":"Effect of tyrosine and phenylalanine supplementation\u0000on the colour and behaviour of chinchillas","authors":"S. Łapiński, S. Pałka, A. Otwinowska-Mindur","doi":"10.22358/jafs/162617/2023","DOIUrl":"https://doi.org/10.22358/jafs/162617/2023","url":null,"abstract":"The long-tailed chinchilla (Chinchilla lanigera Bennett, 1829) is native to northern Chile and can be found along the foothills of the Andes and the coastal mountains south to Talca, at an elevation of 400–1650 m. Human activities, such as poaching, hunting, grazing of cattle and goats, mining, firewood extraction, and the pet trade, have posed a significant threat to this species for years. Although legislation to protect the species has been in place since 1929, it was not efficiently enforced until the establishment of the Reserva Nacional Las Chinchillas in Auco, Chile in 1983. This species has been included in the CITES Appendix I since 1977. The current wild population is estimated at around 5350 mature individuals, and despite current protection measures, populations continue to decline, and the species is classified as endangered according to the Red List Category & Criteria (Jimenez, 1996; Roach and Kennerley, 2016). Currently, chinchillas are a popular pet and farm animal, but due to ABSTRACT. The purpose of the study was to verify whether increasing the content of tyrosine and phenylalanine in the feed would result in more intense colouration of chinchilla fur and affect the animals’ reactions to behavioural tests. Chinchillas were divided into 3 groups: G-1 fed complete commercial fodder (Tyr 6.91 mg/g, Phe 7.45 mg/g), G-2 fed commercial and experimental fodder (1:1 ratio), and G-3 fed with experimental fodder (Tyr 14.31 mg/g, Phe 21.65 mg/g). During the experiment, the fur colouration type was assessed using two methods: objective, with a colorimeter (CIE L*a*b* colour space: L* – light-dark axis, a* – red-green axis, b* – yellow-blue axis, C* – colour saturation), and subjective, by a qualified chinchilla judge. A behavioural hand test was used to categorise the chinchillas’ responses to human intrusion into their cage. The results from the colorimeter measurements did not show any significant differences in the L* (P > 0.05) component; however, significant differences were observed for the a* (P = 0.0029), b* (P = 0.0218), and C* (P = 0.0342) components in relation to the fur colouration of individual groups. The assessment of the colour type by a qualified specialist found statistically significant differences (P < 0.05) between the initial and final measurements. Spearman’s correlation coefficients between colour type assessment and colour components were mostly low or medium, but with stronger values observed for L* compared to a*, b* or C*. There were no significant effects of the feeding group on the results of the and test. However, a decreasing trend was observed in the hand test results for G-3 from week 5 of the measurements. Received: 7 September 2022 Revised: 23 March 2022 Accepted: 25 March 2022","PeriodicalId":14919,"journal":{"name":"Journal of Animal and Feed Sciences","volume":" ","pages":""},"PeriodicalIF":1.0,"publicationDate":"2023-05-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44833535","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-05-19DOI: 10.22358/jafs/161907/2023
T. Norrapoke, T. Pongjongmit, C. Supapong
. The purpose of the study was to investigate the impact of Manila palm supplementation on rumen fermentation and eradication of gastrointestinal nematodes in goats. Sixteen native crossbred goats, with an average weight of 20 ± 3.1 kg were utilised in a randomized complete block design experiment
{"title":"Manila palm extract supplementation improves rumen\u0000fermentation and eliminates gastro-intestinal parasites in goats","authors":"T. Norrapoke, T. Pongjongmit, C. Supapong","doi":"10.22358/jafs/161907/2023","DOIUrl":"https://doi.org/10.22358/jafs/161907/2023","url":null,"abstract":". The purpose of the study was to investigate the impact of Manila palm supplementation on rumen fermentation and eradication of gastrointestinal nematodes in goats. Sixteen native crossbred goats, with an average weight of 20 ± 3.1 kg were utilised in a randomized complete block design experiment","PeriodicalId":14919,"journal":{"name":"Journal of Animal and Feed Sciences","volume":" ","pages":""},"PeriodicalIF":1.0,"publicationDate":"2023-05-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48223728","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-05-18DOI: 10.22358/jafs/162066/2023
Michael Zaid, S. K. Ramiah, E. S. Jamein, I. Zulkifli, I. H. Lokman, F. Amirul, S.A.A. Fadzlin, S. M. Zamri, A. Jayanegara, H. Hassim
. The increasing human population and improved living standards in developing countries have led to a higher demand for animal proteins, which has resulted in increased costs of animal feed ingredients such as fish meal, fish oil, soybean meal
{"title":"Potential use of black soldier fly, Hermetia illucens larvae\u0000in chicken feed as a protein replacer: a review","authors":"Michael Zaid, S. K. Ramiah, E. S. Jamein, I. Zulkifli, I. H. Lokman, F. Amirul, S.A.A. Fadzlin, S. M. Zamri, A. Jayanegara, H. Hassim","doi":"10.22358/jafs/162066/2023","DOIUrl":"https://doi.org/10.22358/jafs/162066/2023","url":null,"abstract":". The increasing human population and improved living standards in developing countries have led to a higher demand for animal proteins, which has resulted in increased costs of animal feed ingredients such as fish meal, fish oil, soybean meal","PeriodicalId":14919,"journal":{"name":"Journal of Animal and Feed Sciences","volume":" ","pages":""},"PeriodicalIF":1.0,"publicationDate":"2023-05-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45946243","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-05-15DOI: 10.22358/jafs/161997/2023
O. Vural, A. Tunç, M. Kamaszewski, E. Aksakal
. ABSTRACT. Royal jelly (RJ) is a bee-derived product widely used as a dietary supplement due to its high potential health benefits. The present study was designed to investigate the effect of feeding RJ on zebrafish fatty acid composition. Zebrafish were fed five distinct diets (D1, D2, D3, D4, and D5) for 56 days supplemented with 0.0, 0.1, 0.4, 1.6, and 6.4% RJ, respectively. Gas chromatography-mass spectrometry and fatty acid methyl ester (FAME) analyses were used to determine FA content in the whole body of zebrafish. The results showed that a feeding regimen that included incrementing RJ doses resulted in statistically significant increases in 16:0, 18:2n-6, and the ratio of eicosapentaenoic acid (EPA) to docosahexaenoic acid (DHA) ( P < 0.05). In particular, the content of EPA/DHA increased up to 1.3-fold compared to control group D1. Conversely, increasing RJ levels led to significant decreases in DHA, HUFA, and n-3/n-6 PUFA levels in relation to group D1 ( P < 0.05). In conclusion, considering the EPA/DHA ratio among the analysed fatty acids, the diet with a 1.6% RJ addition was the optimal choice. Therefore, RJ can be recommended as a sustainable alternative lipid source in aquaculture.
{"title":"Is royal jelly a sustainable alternative lipid source\u0000in aquaculture? Influence of dietary royal jelly levels\u0000on fatty acid composition in zebrafish","authors":"O. Vural, A. Tunç, M. Kamaszewski, E. Aksakal","doi":"10.22358/jafs/161997/2023","DOIUrl":"https://doi.org/10.22358/jafs/161997/2023","url":null,"abstract":". ABSTRACT. Royal jelly (RJ) is a bee-derived product widely used as a dietary supplement due to its high potential health benefits. The present study was designed to investigate the effect of feeding RJ on zebrafish fatty acid composition. Zebrafish were fed five distinct diets (D1, D2, D3, D4, and D5) for 56 days supplemented with 0.0, 0.1, 0.4, 1.6, and 6.4% RJ, respectively. Gas chromatography-mass spectrometry and fatty acid methyl ester (FAME) analyses were used to determine FA content in the whole body of zebrafish. The results showed that a feeding regimen that included incrementing RJ doses resulted in statistically significant increases in 16:0, 18:2n-6, and the ratio of eicosapentaenoic acid (EPA) to docosahexaenoic acid (DHA) ( P < 0.05). In particular, the content of EPA/DHA increased up to 1.3-fold compared to control group D1. Conversely, increasing RJ levels led to significant decreases in DHA, HUFA, and n-3/n-6 PUFA levels in relation to group D1 ( P < 0.05). In conclusion, considering the EPA/DHA ratio among the analysed fatty acids, the diet with a 1.6% RJ addition was the optimal choice. Therefore, RJ can be recommended as a sustainable alternative lipid source in aquaculture.","PeriodicalId":14919,"journal":{"name":"Journal of Animal and Feed Sciences","volume":" ","pages":""},"PeriodicalIF":1.0,"publicationDate":"2023-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45958400","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-04-25DOI: 10.22358/jafs/159458/2023
J. Zhang, Y. Liu, L. Bu, Q. Liu, C. Pei, G. Guo
. The experiment was designed to evaluate the effect of sodium selenite (Na 2 SeO 3 ) on the proliferation of bovine mammary gland epithelial cells (BMECs), and to assess the underlying mechanisms. The addition of Na 2 SeO 3 at doses ranging from 18 to 140 nM stimulated BMEC proliferation. Nevertheless, high doses of Na 2 SeO 3 (> 70 nM) did not further stimulate BMEC proliferation compared to 35 nM Na 2 SeO 3 . Therefore, the addition of 35 nM Na 2 SeO 3 increased the proportion of epithelial cells undergoing DNA replication, mRNA expression of proliferating cell nuclear antigen (PCNA), Cyclin A2
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