Journal of Applied Research on Medicinal and Aromatic Plants最新文献_第6页

Resolving taxonomic complexities in medicinal Aralia: Genome-scale phylogeny and super-barcoding advance species identification and conservation 解决药用植物分类的复杂性：基因组尺度的系统发育和超条形码促进物种鉴定和保护

IF 3.8 2区农林科学 Q1 PLANT SCIENCES

Journal of Applied Research on Medicinal and Aromatic Plants

Pub Date : 2025-05-01 DOI: 10.1016/j.jarmap.2025.100643

Yanan Cao , Menghao Wang , Jiamei Li , Ying Yang , Hao Wang , Yanyan Liu , Hongwei Wang , Risong Na

Aralia section Dimorphanthus represents the largest section in Araliaceae and has been employed in East Asian traditional medicine for centuries. However, the morphological complexity and the prevalence of hybridization/introgression posed a great challenge for accurate species identification, thereby hindering the development, conservation and application of these medicinal plants. In this study, we employed sequences of hundreds of nuclear loci and the complete plastomes to reconstruct the phylogenetic relationships, and evaluated the effectiveness of 12 distinct barcode systems for distinguishing Chinese species of Aralia sect. Dimorphanthus. We found that plastome length, gene content, and order of these species were highly conserved, while the inverted repeat regions showed variability between temperate and tropical/subtropical lineages, providing novel markers for geographical traceability. Phylogenetic relationships constructed based on the genome-scale data confirmed that Chinese Aralia sect. Dimorphanthus was monophyletic and comprised three well-supported lineages. Incomplete lineage sorting and hybridization were identified as the main factors contributing to the phylogenetic conflicts among trees. Species discrimination analysis revealed that super-barcoding strategies integrating complete plastomes and nrDNA sequences, achieved a 92 % species identification accuracy among the 14 studied species of Aralia sect. Dimorphanthus, outperforming standard barcodes. While this system resolved most taxonomic ambiguities, the inclusion of universal nuclear markers is crucial to resolve ambiguities in highly variable taxa like A. elata. These advancements directly support industrial needs for reliable species authentication and biodiversity conservation in pharmacologically important species.

双菊属植物是五加科植物中最大的一种，在东亚的传统医学中已经使用了几个世纪。然而，由于其形态的复杂性和杂交/渗入现象的普遍存在，给准确的物种鉴定带来了很大的挑战，从而阻碍了这些药用植物的开发、保护和应用。本研究利用数百个核位点的序列和完整的质粒进行了系统发育关系的重建，并对12种不同的条形码系统进行了鉴定。研究发现，这些物种的质体体长度、基因含量和序列高度保守，而倒置重复区在温带和热带/亚热带谱系之间表现出差异，为地理溯源提供了新的标记。基于基因组尺度数据构建的系统发育关系证实，中国异花楤木组为单系系，由3个支持良好的谱系组成。谱系分选不全和杂交不全是造成树间系统发育冲突的主要因素。物种鉴别分析表明，采用整合全质体和nrDNA序列的超条形码策略，对菊苣属（Aralia sect. Dimorphanthus） 14种植物的物种鉴定准确率达到92 %，优于标准条形码。虽然该系统解决了大多数分类上的歧义，但通用核标记的包含对于解决像elata这样高度可变的分类群的歧义至关重要。这些进步直接支持工业对可靠的物种认证和生物多样性保护的需求。

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引用次数: 0

Effective SSR markers for assessing population structure, genetic diversity, and volatile compounds in Atractylodis Rhizoma 白术群体结构、遗传多样性和挥发性物质评价的有效SSR标记

IF 3.8 2区农林科学 Q1 PLANT SCIENCES

Journal of Applied Research on Medicinal and Aromatic Plants

Pub Date : 2025-05-01 DOI: 10.1016/j.jarmap.2025.100642

Haotian Zhong , Lina Chen , Lei Chen , Xiao Huang , Ling Gong , Juan Hu , Yuling Zeng , Kun Yu , Lin Sen

In this study, we aimed to develop SSR molecular markers for assessing the population structure and genetic diversity of Atractylodes lancea (AL) and Atractylodes chinensis (AC), with a focus on their volatile compounds. By utilizing transcriptome data from AL, we successfully developed 29 pairs of highly polymorphic SSR primers, generating 264 distinct alleles. Cluster analysis revealed three distinct populations: Mao Mountain, Dabie Mountain, and samples from other locations and primers with high discriminatory power were selected. Additionally, a representative core subset of 106 samples was selected from the original germplasm, capable of representing over 99 % of the genetic variation. This core subset provides an efficient resource for future studies on population genetics and trait association. The Support Vector Machine (SVM)-based method achieved an accuracy of 98.8 % in distinguishing Atractylodis Rhizoma (AR) varieties. GC-MS screening identified 24 potential differential metabolites between the two species, with correlation analysis showing significant association. This study successfully developed SSR molecular markers for analyzing genetic diversity in AL and AC and established a reliable method for identifying AR medicinal varieties. These findings provide a solid foundation for future quality control of AR and the selection of superior germplasm.

本研究旨在利用SSR分子标记对苍术（Atractylodes lancea， AL）和中国苍术（Atractylodes chinensis， AC）的居群结构和遗传多样性进行评价，重点分析其挥发性成分。利用AL的转录组数据，我们成功开发了29对高多态性SSR引物，产生264个不同的等位基因。聚类分析显示，毛山、大别山和其他地区的样本具有明显的种群特征，并选择了判别能力强的引物。此外，从原始种质中选择了106个样本的代表性核心子集，能够代表99% %以上的遗传变异。这一核心亚群为今后的群体遗传学和性状关联研究提供了有效的资源。基于支持向量机（SVM）的方法对苍术品种的鉴别准确率达到98.8 %。GC-MS筛选鉴定出24种潜在的两种差异代谢物，相关分析显示相关性显著。本研究成功开发了用于分析AR和AC遗传多样性的SSR分子标记，为AR药用品种的鉴定建立了可靠的方法。这些研究结果为今后AR的质量控制和优质种质的选择提供了坚实的基础。

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引用次数: 0

Blockchain technology in herbal medicines: Applications, trends, and prospects 区块链技术在草药中的应用、趋势和前景

IF 3.8 2区农林科学 Q1 PLANT SCIENCES

Journal of Applied Research on Medicinal and Aromatic Plants

Pub Date : 2025-05-01 DOI: 10.1016/j.jarmap.2025.100645

Mingyu Han , Qiang Hu , Yuanzhong Wang

The quality and safety of Herbal medicines (HMs) not only affect the development of the industry chain, but also the life safety rights and interests of patients. Traditional HMs supply chains suffer from opaque information and easy tampering, but blockchain intervention can significantly improve the security and traceability of information. The combination of blockchain and herb chain will be the future trend. This paper uses bibliometric statistics to analyze the practical application scenarios of blockchain and summarizes the challenges faced in HMs supply chain. It further explores the application potential of blockchain in the field of HMs and designs a traceability system for HMs based on blockchain technology. A large number of literature surveys found that the application scope of blockchain in HMs is relatively small, and comprehensive coverage of blockchain applications has a high coefficient of difficulty. This paper provides more possibilities for the application of blockchain in HMs, which will promote the development of a benign cycle of the HMs industry chain and protect the rights and interests of consumers.

中药材的质量安全问题不仅关系到产业链的发展，也关系到患者的生命安全权益。传统的医疗器械供应链存在信息不透明和易被篡改的问题，而区块链干预可以显著提高信息的安全性和可追溯性。区块链与草药连锁的结合将是未来的趋势。本文运用文献计量统计学分析了区块链的实际应用场景，总结了医疗保健企业供应链面临的挑战。进一步探索区块链在医疗器械领域的应用潜力，设计了基于区块链技术的医疗器械溯源系统。大量文献调查发现，区块链在HMs中的应用范围相对较小，区块链应用的综合覆盖难度系数较高。本文为区块链在酒店管理行业的应用提供了更多的可能性，促进酒店管理行业产业链良性循环的发展，保护消费者权益。

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引用次数: 0

Xenia effect enhance pod and seed quality in Gleditsia sinensis Xenia效应提高了皂荚和种子的品质

IF 3.8 2区农林科学 Q1 PLANT SCIENCES

Journal of Applied Research on Medicinal and Aromatic Plants

Pub Date : 2025-05-01 DOI: 10.1016/j.jarmap.2025.100644

Qingying Zeng , Yang Zhao , Xiurong Wang , Meixuan Xie , Fuhua Liu , Xingfu Zhang

Gleditsia sinensis is a tree species with high economic value, but the pairing of male and female trees is often overlooked in its cultivation practices. The fruit quality of G. sinensis under natural pollination tends to be inconsistent, resulting in lower economic value. Therefore, understanding the xenia effects of G. sinensis on fruit and seed quality is crucial for improving their overall quality. In this study, The G. sinensis as the female parent and Gleditsia japonica var. delavayi, Gleditsia japonica, G. sinensis, and Gleditsia fera as male parents for hybridization. By observing and comparing the inflorescence characteristics of different male parents, as well as the fruit set rates, pod phenotypes, and seed yield under various pollination combinations, we found that artificial pollination significantly increased the yield of pods and seeds, and distinct xenia effects were observed in the fruits and seeds of G. sinensis. Through comprehensive evaluation, G. fera was identified as the optimal pollen source for interspecific hybridization with G. sinensis, significantly increasing fruit set rate, saponin content, and seed yield. Pollination with G. japonica resulted in a higher seed abortion rate and poorer seed quality, but significantly increased the amino acid and starch content in the pods. G. delavayi pollen is more inclined to produce high-quality seeds. These findings provide valuable insights for the selection of G. sinensis pollinating trees and the improvement of G. sinensis fruit quality.

Data availability

All data generated in this study are presented within the manuscript. Further information and requests could be directed to the lead contact, Prof. Yang Zhao ([email protected])

皂荚是一种具有较高经济价值的树种，但在皂荚的栽培实践中，往往忽略了雌雄树的配对。在自然授粉条件下，白杨果实品质趋于不一致，经济价值较低。因此，了解赤霉素对赤霉素果实和种子品质的影响，对提高赤霉素果实和种子的整体品质具有重要意义。本研究以中国皂荚为雌性亲本，皂荚为雄性亲本，对皂荚变种delavayi、皂荚、中国皂荚和fera皂荚进行杂交。通过观察和比较不同父本的花序特征，以及不同授粉组合下的坐果率、荚果表型和种子产量，我们发现人工授粉显著提高了白杨的荚果和种子产量，并且在白杨的果实和种子中观察到明显的异种效应。通过综合评价，确定fera是与sinensis进行种间杂交的最佳花粉源，可以显著提高坐果率、皂苷含量和种子产量。用粳稻授粉后，籽粒败育率较高，籽粒质量较差，但荚果中氨基酸和淀粉含量显著增加。花粉更倾向于产生优质种子。这些研究结果为中华猕猴桃授粉树种的选择和中华猕猴桃果实品质的改良提供了有价值的见解。数据可得性本研究中产生的所有数据均在论文中列出。进一步的信息和请求可以直接联系首席联系人赵杨教授（[email protected]）。

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引用次数: 0

Characterizing trait-specific populations and genetic diversity patterns in clary sage (Salvia sclarea L.) using SSR markers 利用SSR标记分析鼠尾草（Salvia sclarea L.）的性状特异性群体和遗传多样性模式

IF 3.8 2区农林科学 Q1 PLANT SCIENCES

Journal of Applied Research on Medicinal and Aromatic Plants

Pub Date : 2025-05-01 DOI: 10.1016/j.jarmap.2025.100646

Niketa Yadav , Mohar Singh , Asmita Saini , Pratibha Pandey , Garima Thakur , Ramesh Chauhan , Satbeer Singh , Sanatsujat Singh

Clary sage (Salvia sclarea L.) is an aromatic and medicinal herb grown widely across Europe, Western Asia, Maghreb, the Mideast, and countries like India. The purpose of this study was to assess genetic diversity of clary sage genotypes from the Western Himalayas, aiming to support future breeding efforts. A total of 72 genotypes of clary sage from six distinct populations were examined using 49 simple sequence repeat markers. In total, 323 alleles were detected ranging from 1 to 16 with an average of 6.54 alleles per locus. Among these, 78.33 % were common alleles, 1.86 % were rare, and 19.81 % were frequent alleles. The results revealed an overall polymorphic information content of 93.88 %, with an average of 0.71 ranging from 0.00 to 0.93 at each marker locus, indicating high genetic variability among the studied material. The detected heterozygosity (0.52) was lower than expected heterozygosity (0.71), pointing a heterozygotes deficit populations. Analysis of variance showed considerable variability for biomass yield. Molecular analysis of variance (AMOVA) showed that genetic diversity was greater within individuals than among populations. Unweighted neighbour-joining, principal coordinate analysis, and population structure showed that the genotypes from different geographic regions and various biomass yield groups were predominantly admixed across clusters. The cluster-III represented the Bharmour population was the highest biomass performer group. The observed diversity patterns will facilitate plant breeders to make choices for breeding programs aiming for higher biomass yield in this plant.

鼠尾草（Salvia sclarea L.）是一种芳香和药用草本植物，广泛生长在欧洲、西亚、马格里布、中东和印度等国家。本研究的目的是评估西喜马拉雅地区鼠尾草基因型的遗传多样性，旨在为今后的育种工作提供支持。利用49个简单序列重复标记对6个不同居群的鼠尾草共72个基因型进行了分析。共检测到1 ~ 16个等位基因323个，平均每个位点检测到6.54个等位基因。其中78.33 %为常见等位基因，1.86 %为罕见等位基因，19.81 %为常见等位基因。结果表明，总体多态性信息含量为93.88 %，每个标记位点的平均多态性信息含量为0.71，范围在0.00 ~ 0.93之间，表明材料间具有较高的遗传变异性。检测到的杂合度（0.52）低于预期的杂合度（0.71），表明存在杂合度不足的群体。方差分析表明，生物量产量具有相当大的变异性。分子变异分析（AMOVA）表明，遗传多样性在个体内大于群体间。非加权邻联分析、主坐标分析和种群结构分析表明，不同地理区域和不同生物量产量群体的基因型在集群间以混合为主。聚类iii代表巴摩种群是生物量表现最高的群体。观察到的多样性模式将有助于植物育种者选择以提高该植物生物量产量为目标的育种计划。

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引用次数: 0

Assessment of the quality of plant material in commercial products of ground ivy herb (Glechomae hederaceae herba) 藤属植物商品原料质量评价（英文）

IF 3.8 2区农林科学 Q1 PLANT SCIENCES

Journal of Applied Research on Medicinal and Aromatic Plants

Pub Date : 2025-04-01 DOI: 10.1016/j.jarmap.2025.100634

Karolina Grabowska, Weronika Ziółkowska-Żygas, Agnieszka Galanty, Dagmara Wróbel-Biedrawa, Irma Podolak

G. hederacea has a well-established position in traditional medicinal systems of Europe and Asia. Despite the significant increase in the number of products based on this plant on the European market, there is as yet no European Pharmacopoeia monograph on Glechomae herba, therefore legally binding quality standards of medical-grade ground ivy herb in member states that ratified Convention on the elaboration of a European Pharmacopoeia. This study aimed to investigate differences in the quality of the commercially available single-herb loose G. hederacea products in the aspects of authentication, purity of plant material and content of bioactive substances. As the therapeutic potential of G. hederacea is related to the presence of phenolic compounds, the study on qualitative and quantitative analysis of phenolics by HPLC method was performed. The analysis revealed significant differences in the quality of ground ivy plant material, in terms of the content of bioactive compounds. The determined levels of rosmarinic acid, chlorogenic acid, gallic acid and rutin in the products ranged from 0.10 to 4.55 mg/g DW; 0.18–1.57 mg/g DW; 0.02–1.28 mg/g DW; to 6.01 mg/g DW, respectively. Additionally, the products varied significantly in the sum of determined polyphenols (1.5 – 7.16 mg/g DW). The findings highlight the need to establish the standards and requirements for bioactive phenolic content to ensure high-quality plant material with significant medicinal value. Additionally, our study suggests that rosmarinic acid, rutin, chlorogenic acid, and the total polyphenol content could serve as quality markers for Glechomae hederaceae herba in pro-health applications.

在欧洲和亚洲的传统医药体系中，白头翁有着良好的地位。尽管欧洲市场上以这种植物为基础的产品数量显著增加，但迄今为止还没有关于Glechomae herba的欧洲药典专著，因此，在批准《制定欧洲药典公约》的成员国中，具有法律约束力的医疗级地面常春藤草药质量标准。本研究旨在探讨市售单一药材麻花散装产品在鉴定、原料纯度和生物活性物质含量等方面的质量差异。由于麻属植物的治疗潜力与酚类化合物的存在有关，因此采用高效液相色谱法对麻属植物中的酚类物质进行了定性和定量分析。分析表明，在生物活性化合物含量方面，地面常春藤植物材料的质量存在显著差异。产品中迷迭香酸、绿原酸、没食子酸和芦丁的含量为0.10 ~ 4.55 mg/g DW；0.18 - -1.57 毫克/克DW,0.02 - -1.28 毫克/克DW,分别为6.01 mg/g DW。此外，两种产品的多酚含量差异显著（1.5 - 7.16 mg/g DW）。研究结果表明，有必要建立生物活性酚含量的标准和要求，以确保具有重要药用价值的高质量植物材料。此外，本研究提示迷迭香酸、芦丁、绿原酸和总多酚含量可作为中药的质量评价指标。

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引用次数: 0

Identification of Dendrobium officinale species and authentication of commercial products by DNA barcoding and high-resolution melting analysis 利用DNA条形码和高分辨率熔融分析鉴定铁皮石斛的种类及商品鉴定

IF 3.8 2区农林科学 Q1 PLANT SCIENCES

Journal of Applied Research on Medicinal and Aromatic Plants

Pub Date : 2025-04-01 DOI: 10.1016/j.jarmap.2025.100633

Zhilong He , Zhao Yang , Cui Dong , Shanshan Yan , Jing Lin , Hexigeduleng Bao , Fan Tang , Leilei Zhang , Cheng Zhu , Yan Hu , Yanfei Ding

Dendrobium officinale is widely used as a precious traditional medicinal plant and health food in China. However, because of its high nutritional and medicinal value, it is often targeted for adulteration or mislabeling. This work aims to exploit DNA barcoding combined with high-resolution melting analysis to differentiate Dendrobium officinale（D. officinale）species from non-D. officinale species and to further identify its botanical origin. The internal transcribed spacer 2 (ITS2) was targeted as a candidate barcode for Dendrobium species identification. High resolution melting（HRM）method allowed differentiating the six Dendrobium species in three clusters with 95 % confidence interval, which is consistent with the sequencing analysis. ITS2 sequencing results implied that 43 % of commercial Dendrobium products may be mislabeled. Thus, ITS2 DNA barcoding with HRM assay could be used as a simple, specific and cost-effective tool to identify different Dendrobium species and commercial Dendrobium products, and to authenticate the origin of D. officinale.

铁皮石斛是一种珍贵的传统药用植物和保健食品，在中国被广泛使用。然而，由于其高营养和药用价值，它经常成为掺假或贴错标签的目标。本工作旨在利用DNA条形码结合高分辨率熔融分析来区分铁皮石斛（ddrobium officinale）。officinale)来自非d。Officinale的种类，并进一步确定其植物来源。内部转录间隔器2 （ITS2）被定位为石斛物种鉴定的候选条形码。高分辨率熔融（HRM）方法可将6种石斛分为3个簇，置信区间为95 %，与测序分析结果一致。ITS2测序结果表明43 %的石斛商业产品可能存在标签错误。因此，ITS2 DNA条形码与HRM技术可作为一种简单、特异、经济的工具，用于不同石斛品种和商品石斛产品的鉴定，并对铁皮石斛的来源进行鉴定。

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引用次数: 0

Accurate and intelligent quantification of adulterated Angelicae Sinensis Radix by a novel ensemble method with near-infrared spectroscopy 新型近红外光谱系综法准确智能定量掺假当归

IF 3.8 2区农林科学 Q1 PLANT SCIENCES

Journal of Applied Research on Medicinal and Aromatic Plants

Pub Date : 2025-04-01 DOI: 10.1016/j.jarmap.2025.100640

Xihui Bian , Yuxia Liu , Jiaqi Xie , Chunyan Zhao , Xiaoyao Tan , Weilu Tian

The integrity and authenticity of Chinese medicinal materials are essential for ensuring the efficacy of medicines and safety of patients. Angelicae Sinensis Radix (ASR), a highly valued traditional Chinese medicine (TCM), is often substituted or mixed with lower-cost alternatives. In this study, a novel method was proposed for the quantification of adulterated ASR using near-infrared (NIR) spectroscopy combined with chemometrics. Binary and ternary adulterated ASR samples were prepared by mixing ASR with Angelicae Pubescentis Radix (APR) and Chuanxiong Rhizoma (CR), and their NIR spectra were measured in the range of 1000–1800 nm. Sample subsets were initially selected using Monte Carlo (MC) sampling, followed by the application of whale optimization algorithm (WOA) to select variables and establish an extreme learning machine (ELM) model, referred to as MC-WOA-ELM. The iteration number and percentage of training subsets for MC-WOA-ELM were optimized to further improve the predictive performance of model. The predictive ability of the proposed method was compared with partial least squares (PLS), ELM and WOA-ELM for both binary and ternary adulterated ASR samples. The results indicate that MC-WOA-ELM achieves the highest prediction accuracy. Therefore, the proposed method is rapid and intelligent for the quantification of adulterated ASR samples.

中药材的完整性和真实性对保证药品的疗效和患者的安全至关重要。当归（Angelicae Sinensis Radix， ASR）是一种价值很高的中药，经常被替代或与较低成本的替代品混合使用。本研究提出了一种利用近红外光谱与化学计量学相结合的方法来定量掺假ASR。将ASR与短毛当归（APR）和川芎（CR）混合制备二元和三元掺假ASR样品，并在1000 ~ 1800 nm范围内测定其近红外光谱。首先采用蒙特卡罗（Monte Carlo， MC）采样方法选择样本子集，然后应用鲸鱼优化算法（whale optimization algorithm， WOA）选择变量，建立极限学习机（extreme learning machine， ELM）模型，简称MC-WOA-ELM。对MC-WOA-ELM的训练子集迭代次数和百分比进行优化，进一步提高模型的预测性能。将该方法与偏最小二乘（PLS）、ELM和WOA-ELM对二元和三元掺假ASR样品的预测能力进行了比较。结果表明，MC-WOA-ELM预测精度最高。因此，该方法具有快速、智能化的特点，可用于ASR掺杂样品的定量分析。

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引用次数: 0

Heavy metal pollution in Iranian medicinal plants, a review of sources, distribution, and health implications 伊朗药用植物中的重金属污染：来源、分布和健康影响的综述

IF 3.8 2区农林科学 Q1 PLANT SCIENCES

Journal of Applied Research on Medicinal and Aromatic Plants

Pub Date : 2025-04-01 DOI: 10.1016/j.jarmap.2025.100637

Farzaneh Mirzabayati, Amir Hossein Hamidian

Environmental pollution, particularly with heavy metals, is a critical issue in assessing the quality of medicinal plants. The concentration of heavy metals in these plants depends on factors such as growth environment, species, and processing methods. Contaminants absorbed by plants can enter the human body through consumption. This review examines the impact of heavy metals—cadmium (Cd), lead (Pb), arsenic (As), copper (Cu), chromium (Cr), zinc (Zn), and nickel (Ni)—on medicinal plants in Iran. It explores how environmental contamination affects plant quality and analyzes trends in measurement techniques and metal concentrations over time. Results show that Cd has the most significant negative impact on plant growth (63 %), followed by Pb (21 %), Cr (11 %), and Zn (5 %). Cd exceeded WHO limits in 47 % of samples, while Pb and Zn exceeded limits in 26 % and 20 %, respectively. Cd and Pb also significantly reduced germination rates. Contamination with Cd and Pb poses serious health risks, including kidney damage, bone disorders, cancer, neurological issues, and reproductive harm. Strict quality control, sustainable farming, and public awareness are essential to mitigate these risks. This study highlights the need for further research, particularly in industrial regions like Tehran, Khuzestan, Isfahan, and Fars, to ensure the safety of medicinal plants.

环境污染，特别是重金属污染，是评估药用植物质量的一个关键问题。这些植物体内重金属的浓度与生长环境、种类、加工方法等因素有关。被植物吸收的污染物可以通过食用进入人体。本文综述了重金属镉（Cd）、铅（Pb）、砷（As）、铜（Cu）、铬（Cr）、锌（Zn）和镍（Ni）对伊朗药用植物的影响。它探讨了环境污染如何影响植物质量，并分析了测量技术和金属浓度随时间的变化趋势。结果表明，镉对植物生长的影响最显著（63 %），其次是Pb（21 %）、Cr（11 %）和Zn（5 %）。样品中镉超标的比例为47. %，铅和锌超标的比例分别为26. %和20. %。Cd和Pb也显著降低了发芽率。镉和铅污染会带来严重的健康风险，包括肾损伤、骨骼疾病、癌症、神经问题和生殖危害。严格的质量控制、可持续农业和公众意识对于减轻这些风险至关重要。这项研究强调需要进一步研究，特别是在德黑兰、胡泽斯坦、伊斯法罕和法尔斯等工业区，以确保药用植物的安全。

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引用次数: 0

Comparative analysis of three extraction methods for ginger leaf polysaccharide: Chemical composition, antioxidant activity, and hypolipidemic effects 姜叶多糖三种提取方法的化学成分、抗氧化活性和降血脂作用的比较分析

IF 3.8 2区农林科学 Q1 PLANT SCIENCES

Journal of Applied Research on Medicinal and Aromatic Plants

Pub Date : 2025-04-01 DOI: 10.1016/j.jarmap.2025.100639

Zhong-hao Zhang , Zhong-hua Zhao , Tian-yi Cao , Cai-rong Yang , Zi-zhong Tang , Shi-ling Feng , Shu Yuan , Sheng-lin Hu , Ming Yuan

Ginger leaves are often discarded in agriculture, exacerbating continuous cropping obstacles. Polysaccharide, due to their low toxicity and diverse bioactivities, have garnered considerable interest. To isolate ginger leaf polysaccharide (GLP), we compared three extraction methods: hot water extraction, ultrasonic-assisted hot water extraction, and ultrasonic-assisted aqueous two-phase system extraction. Response surface methodology optimization revealed ultrasonic-assisted hot water extraction as the most efficient method, achieving a yield of 9.31 % under a liquid-solid ratio of 30 mL/g and an ultrasonic power of 150 W at 65 ℃ for 22 min. Polysaccharide from different extraction methods showed difference in monosaccharide composition and molecular weight. The ginger leaf polysaccharide demonstrated strong antioxidant in vitro and hypolipidemic activities in Caenorhabditis elegans, and that from the ultrasonic-assisted hot water method showed the highest bioactivity. These findings highlight ultrasonic-assisted hot water extraction as the optimal method for efficient extraction of GLP and discover GLP’s potential for pharmaceutical and functional food applications.

姜叶在农业中经常被丢弃，加剧了连作障碍。多糖由于其低毒性和多样化的生物活性而引起了人们的广泛关注。为了分离生姜叶多糖（GLP），比较了热水提取、超声辅助热水提取和超声辅助双水相萃取三种提取方法。响应面优化结果表明，在液固比为30 mL/g、超声功率为150 W、温度为65℃、萃取时间为22 min的条件下，超声辅助热水萃取法的提取率为9.31 %。不同提取方法提取的多糖单糖组成和分子量存在差异。生姜叶多糖对秀丽隐杆线虫具有较强的体外抗氧化和降血脂活性，其中超声辅助热水法提取的活性最高。这些发现强调了超声辅助热水提取法是高效提取GLP的最佳方法，并发现了GLP在制药和功能食品中的应用潜力。

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