Pub Date : 2025-05-01DOI: 10.1016/j.jarmap.2025.100645
Mingyu Han , Qiang Hu , Yuanzhong Wang
The quality and safety of Herbal medicines (HMs) not only affect the development of the industry chain, but also the life safety rights and interests of patients. Traditional HMs supply chains suffer from opaque information and easy tampering, but blockchain intervention can significantly improve the security and traceability of information. The combination of blockchain and herb chain will be the future trend. This paper uses bibliometric statistics to analyze the practical application scenarios of blockchain and summarizes the challenges faced in HMs supply chain. It further explores the application potential of blockchain in the field of HMs and designs a traceability system for HMs based on blockchain technology. A large number of literature surveys found that the application scope of blockchain in HMs is relatively small, and comprehensive coverage of blockchain applications has a high coefficient of difficulty. This paper provides more possibilities for the application of blockchain in HMs, which will promote the development of a benign cycle of the HMs industry chain and protect the rights and interests of consumers.
{"title":"Blockchain technology in herbal medicines: Applications, trends, and prospects","authors":"Mingyu Han , Qiang Hu , Yuanzhong Wang","doi":"10.1016/j.jarmap.2025.100645","DOIUrl":"10.1016/j.jarmap.2025.100645","url":null,"abstract":"<div><div>The quality and safety of Herbal medicines (HMs) not only affect the development of the industry chain, but also the life safety rights and interests of patients. Traditional HMs supply chains suffer from opaque information and easy tampering, but blockchain intervention can significantly improve the security and traceability of information. The combination of blockchain and herb chain will be the future trend. This paper uses bibliometric statistics to analyze the practical application scenarios of blockchain and summarizes the challenges faced in HMs supply chain. It further explores the application potential of blockchain in the field of HMs and designs a traceability system for HMs based on blockchain technology. A large number of literature surveys found that the application scope of blockchain in HMs is relatively small, and comprehensive coverage of blockchain applications has a high coefficient of difficulty. This paper provides more possibilities for the application of blockchain in HMs, which will promote the development of a benign cycle of the HMs industry chain and protect the rights and interests of consumers.</div></div>","PeriodicalId":15136,"journal":{"name":"Journal of Applied Research on Medicinal and Aromatic Plants","volume":"47 ","pages":"Article 100645"},"PeriodicalIF":3.8,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144588674","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-05-01DOI: 10.1016/j.jarmap.2025.100644
Qingying Zeng , Yang Zhao , Xiurong Wang , Meixuan Xie , Fuhua Liu , Xingfu Zhang
Gleditsia sinensis is a tree species with high economic value, but the pairing of male and female trees is often overlooked in its cultivation practices. The fruit quality of G. sinensis under natural pollination tends to be inconsistent, resulting in lower economic value. Therefore, understanding the xenia effects of G. sinensis on fruit and seed quality is crucial for improving their overall quality. In this study, The G. sinensis as the female parent and Gleditsia japonica var. delavayi, Gleditsia japonica, G. sinensis, and Gleditsia fera as male parents for hybridization. By observing and comparing the inflorescence characteristics of different male parents, as well as the fruit set rates, pod phenotypes, and seed yield under various pollination combinations, we found that artificial pollination significantly increased the yield of pods and seeds, and distinct xenia effects were observed in the fruits and seeds of G. sinensis. Through comprehensive evaluation, G. fera was identified as the optimal pollen source for interspecific hybridization with G. sinensis, significantly increasing fruit set rate, saponin content, and seed yield. Pollination with G. japonica resulted in a higher seed abortion rate and poorer seed quality, but significantly increased the amino acid and starch content in the pods. G. delavayi pollen is more inclined to produce high-quality seeds. These findings provide valuable insights for the selection of G. sinensis pollinating trees and the improvement of G. sinensis fruit quality.
Data availability
All data generated in this study are presented within the manuscript. Further information and requests could be directed to the lead contact, Prof. Yang Zhao ([email protected])
{"title":"Xenia effect enhance pod and seed quality in Gleditsia sinensis","authors":"Qingying Zeng , Yang Zhao , Xiurong Wang , Meixuan Xie , Fuhua Liu , Xingfu Zhang","doi":"10.1016/j.jarmap.2025.100644","DOIUrl":"10.1016/j.jarmap.2025.100644","url":null,"abstract":"<div><div><em>Gleditsia sinensis</em> is a tree species with high economic value, but the pairing of male and female trees is often overlooked in its cultivation practices. The fruit quality of <em>G. sinensis</em> under natural pollination tends to be inconsistent, resulting in lower economic value. Therefore, understanding the xenia effects of <em>G. sinensis</em> on fruit and seed quality is crucial for improving their overall quality. In this study, The <em>G. sinensis</em> as the female parent and <em>Gleditsia japonica var. delavayi</em>, <em>Gleditsia japonica</em>, <em>G. sinensis</em>, and <em>Gleditsia fera</em> as male parents for hybridization. By observing and comparing the inflorescence characteristics of different male parents, as well as the fruit set rates, pod phenotypes, and seed yield under various pollination combinations, we found that artificial pollination significantly increased the yield of pods and seeds, and distinct xenia effects were observed in the fruits and seeds of <em>G. sinensis</em>. Through comprehensive evaluation, <em>G. fera</em> was identified as the optimal pollen source for interspecific hybridization with <em>G. sinensis</em>, significantly increasing fruit set rate, saponin content, and seed yield. Pollination with <em>G. japonica</em> resulted in a higher seed abortion rate and poorer seed quality, but significantly increased the amino acid and starch content in the pods. <em>G. delavayi</em> pollen is more inclined to produce high-quality seeds. These findings provide valuable insights for the selection of <em>G. sinensis</em> pollinating trees and the improvement of <em>G. sinensis</em> fruit quality.</div></div><div><h3>Data availability</h3><div>All data generated in this study are presented within the manuscript. Further information and requests could be directed to the lead contact, Prof. Yang Zhao ([email protected])</div></div>","PeriodicalId":15136,"journal":{"name":"Journal of Applied Research on Medicinal and Aromatic Plants","volume":"47 ","pages":"Article 100644"},"PeriodicalIF":3.8,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144502269","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Clary sage (Salvia sclarea L.) is an aromatic and medicinal herb grown widely across Europe, Western Asia, Maghreb, the Mideast, and countries like India. The purpose of this study was to assess genetic diversity of clary sage genotypes from the Western Himalayas, aiming to support future breeding efforts. A total of 72 genotypes of clary sage from six distinct populations were examined using 49 simple sequence repeat markers. In total, 323 alleles were detected ranging from 1 to 16 with an average of 6.54 alleles per locus. Among these, 78.33 % were common alleles, 1.86 % were rare, and 19.81 % were frequent alleles. The results revealed an overall polymorphic information content of 93.88 %, with an average of 0.71 ranging from 0.00 to 0.93 at each marker locus, indicating high genetic variability among the studied material. The detected heterozygosity (0.52) was lower than expected heterozygosity (0.71), pointing a heterozygotes deficit populations. Analysis of variance showed considerable variability for biomass yield. Molecular analysis of variance (AMOVA) showed that genetic diversity was greater within individuals than among populations. Unweighted neighbour-joining, principal coordinate analysis, and population structure showed that the genotypes from different geographic regions and various biomass yield groups were predominantly admixed across clusters. The cluster-III represented the Bharmour population was the highest biomass performer group. The observed diversity patterns will facilitate plant breeders to make choices for breeding programs aiming for higher biomass yield in this plant.
{"title":"Characterizing trait-specific populations and genetic diversity patterns in clary sage (Salvia sclarea L.) using SSR markers","authors":"Niketa Yadav , Mohar Singh , Asmita Saini , Pratibha Pandey , Garima Thakur , Ramesh Chauhan , Satbeer Singh , Sanatsujat Singh","doi":"10.1016/j.jarmap.2025.100646","DOIUrl":"10.1016/j.jarmap.2025.100646","url":null,"abstract":"<div><div>Clary sage (<em>Salvia sclarea</em> L.) is an aromatic and medicinal herb grown widely across Europe, Western Asia, Maghreb, the Mideast, and countries like India. The purpose of this study was to assess genetic diversity of clary sage genotypes from the Western Himalayas, aiming to support future breeding efforts. A total of 72 genotypes of clary sage from six distinct populations were examined using 49 simple sequence repeat markers. In total, 323 alleles were detected ranging from 1 to 16 with an average of 6.54 alleles per locus. Among these, 78.33 % were common alleles, 1.86 % were rare, and 19.81 % were frequent alleles. The results revealed an overall polymorphic information content of 93.88 %, with an average of 0.71 ranging from 0.00 to 0.93 at each marker locus, indicating high genetic variability among the studied material. The detected heterozygosity (0.52) was lower than expected heterozygosity (0.71), pointing a heterozygotes deficit populations. Analysis of variance showed considerable variability for biomass yield. Molecular analysis of variance (AMOVA) showed that genetic diversity was greater within individuals than among populations. Unweighted neighbour-joining, principal coordinate analysis, and population structure showed that the genotypes from different geographic regions and various biomass yield groups were predominantly admixed across clusters. The cluster-III represented the Bharmour population was the highest biomass performer group. The observed diversity patterns will facilitate plant breeders to make choices for breeding programs aiming for higher biomass yield in this plant.</div></div>","PeriodicalId":15136,"journal":{"name":"Journal of Applied Research on Medicinal and Aromatic Plants","volume":"47 ","pages":"Article 100646"},"PeriodicalIF":3.8,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144614605","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
G. hederacea has a well-established position in traditional medicinal systems of Europe and Asia. Despite the significant increase in the number of products based on this plant on the European market, there is as yet no European Pharmacopoeia monograph on Glechomae herba, therefore legally binding quality standards of medical-grade ground ivy herb in member states that ratified Convention on the elaboration of a European Pharmacopoeia. This study aimed to investigate differences in the quality of the commercially available single-herb loose G. hederacea products in the aspects of authentication, purity of plant material and content of bioactive substances. As the therapeutic potential of G. hederacea is related to the presence of phenolic compounds, the study on qualitative and quantitative analysis of phenolics by HPLC method was performed. The analysis revealed significant differences in the quality of ground ivy plant material, in terms of the content of bioactive compounds. The determined levels of rosmarinic acid, chlorogenic acid, gallic acid and rutin in the products ranged from 0.10 to 4.55 mg/g DW; 0.18–1.57 mg/g DW; 0.02–1.28 mg/g DW; to 6.01 mg/g DW, respectively. Additionally, the products varied significantly in the sum of determined polyphenols (1.5 – 7.16 mg/g DW). The findings highlight the need to establish the standards and requirements for bioactive phenolic content to ensure high-quality plant material with significant medicinal value. Additionally, our study suggests that rosmarinic acid, rutin, chlorogenic acid, and the total polyphenol content could serve as quality markers for Glechomae hederaceae herba in pro-health applications.
{"title":"Assessment of the quality of plant material in commercial products of ground ivy herb (Glechomae hederaceae herba)","authors":"Karolina Grabowska, Weronika Ziółkowska-Żygas, Agnieszka Galanty, Dagmara Wróbel-Biedrawa, Irma Podolak","doi":"10.1016/j.jarmap.2025.100634","DOIUrl":"10.1016/j.jarmap.2025.100634","url":null,"abstract":"<div><div><em>G. hederacea</em> has a well-established position in traditional medicinal systems of Europe and Asia. Despite the significant increase in the number of products based on this plant on the European market, there is as yet no European Pharmacopoeia monograph on <em>Glechomae herba,</em> therefore legally binding quality standards of medical-grade ground ivy herb in member states that ratified Convention on the elaboration of a European Pharmacopoeia. This study aimed to investigate differences in the quality of the commercially available single-herb loose <em>G. hederacea</em> products in the aspects of authentication, purity of plant material and content of bioactive substances. As the therapeutic potential of <em>G. hederacea</em> is related to the presence of phenolic compounds, the study on qualitative and quantitative analysis of phenolics by HPLC method was performed. The analysis revealed significant differences in the quality of ground ivy plant material, in terms of the content of bioactive compounds. The determined levels of rosmarinic acid, chlorogenic acid, gallic acid and rutin in the products ranged from 0.10 to 4.55 mg/g DW; 0.18–1.57 mg/g DW; 0.02–1.28 mg/g DW; to 6.01 mg/g DW, respectively. Additionally, the products varied significantly in the sum of determined polyphenols (1.5 – 7.16 mg/g DW). The findings highlight the need to establish the standards and requirements for bioactive phenolic content to ensure high-quality plant material with significant medicinal value. Additionally, our study suggests that rosmarinic acid, rutin, chlorogenic acid, and the total polyphenol content could serve as quality markers for <em>Glechomae hederaceae herba</em> in pro-health applications.</div></div>","PeriodicalId":15136,"journal":{"name":"Journal of Applied Research on Medicinal and Aromatic Plants","volume":"46 ","pages":"Article 100634"},"PeriodicalIF":3.8,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143918450","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-04-01DOI: 10.1016/j.jarmap.2025.100633
Zhilong He , Zhao Yang , Cui Dong , Shanshan Yan , Jing Lin , Hexigeduleng Bao , Fan Tang , Leilei Zhang , Cheng Zhu , Yan Hu , Yanfei Ding
Dendrobium officinale is widely used as a precious traditional medicinal plant and health food in China. However, because of its high nutritional and medicinal value, it is often targeted for adulteration or mislabeling. This work aims to exploit DNA barcoding combined with high-resolution melting analysis to differentiate Dendrobium officinale(D. officinale)species from non-D. officinale species and to further identify its botanical origin. The internal transcribed spacer 2 (ITS2) was targeted as a candidate barcode for Dendrobium species identification. High resolution melting(HRM)method allowed differentiating the six Dendrobium species in three clusters with 95 % confidence interval, which is consistent with the sequencing analysis. ITS2 sequencing results implied that 43 % of commercial Dendrobium products may be mislabeled. Thus, ITS2 DNA barcoding with HRM assay could be used as a simple, specific and cost-effective tool to identify different Dendrobium species and commercial Dendrobium products, and to authenticate the origin of D. officinale.
{"title":"Identification of Dendrobium officinale species and authentication of commercial products by DNA barcoding and high-resolution melting analysis","authors":"Zhilong He , Zhao Yang , Cui Dong , Shanshan Yan , Jing Lin , Hexigeduleng Bao , Fan Tang , Leilei Zhang , Cheng Zhu , Yan Hu , Yanfei Ding","doi":"10.1016/j.jarmap.2025.100633","DOIUrl":"10.1016/j.jarmap.2025.100633","url":null,"abstract":"<div><div><em>Dendrobium officinale</em> is widely used as a precious traditional medicinal plant and health food in China. However, because of its high nutritional and medicinal value, it is often targeted for adulteration or mislabeling. This work aims to exploit DNA barcoding combined with high-resolution melting analysis to differentiate <em>Dendrobium officinale</em><strong>(</strong><em>D. officinale</em>)species from non-<em>D. officinale</em> species and to further identify its botanical origin. The internal transcribed spacer 2 (ITS2) was targeted as a candidate barcode for <em>Dendrobium</em> species identification. High resolution melting(HRM)method allowed differentiating the six <em>Dendrobium</em> species in three clusters with 95 % confidence interval, which is consistent with the sequencing analysis. ITS2 sequencing results implied that 43 % of commercial <em>Dendrobium</em> products may be mislabeled. Thus, ITS2 DNA barcoding with HRM assay could be used as a simple, specific and cost-effective tool to identify different <em>Dendrobium</em> species and commercial <em>Dendrobium</em> products, and to authenticate the origin of <em>D. officinale.</em></div></div>","PeriodicalId":15136,"journal":{"name":"Journal of Applied Research on Medicinal and Aromatic Plants","volume":"46 ","pages":"Article 100633"},"PeriodicalIF":3.8,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143918451","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-04-01DOI: 10.1016/j.jarmap.2025.100640
Xihui Bian , Yuxia Liu , Jiaqi Xie , Chunyan Zhao , Xiaoyao Tan , Weilu Tian
The integrity and authenticity of Chinese medicinal materials are essential for ensuring the efficacy of medicines and safety of patients. Angelicae Sinensis Radix (ASR), a highly valued traditional Chinese medicine (TCM), is often substituted or mixed with lower-cost alternatives. In this study, a novel method was proposed for the quantification of adulterated ASR using near-infrared (NIR) spectroscopy combined with chemometrics. Binary and ternary adulterated ASR samples were prepared by mixing ASR with Angelicae Pubescentis Radix (APR) and Chuanxiong Rhizoma (CR), and their NIR spectra were measured in the range of 1000–1800 nm. Sample subsets were initially selected using Monte Carlo (MC) sampling, followed by the application of whale optimization algorithm (WOA) to select variables and establish an extreme learning machine (ELM) model, referred to as MC-WOA-ELM. The iteration number and percentage of training subsets for MC-WOA-ELM were optimized to further improve the predictive performance of model. The predictive ability of the proposed method was compared with partial least squares (PLS), ELM and WOA-ELM for both binary and ternary adulterated ASR samples. The results indicate that MC-WOA-ELM achieves the highest prediction accuracy. Therefore, the proposed method is rapid and intelligent for the quantification of adulterated ASR samples.
{"title":"Accurate and intelligent quantification of adulterated Angelicae Sinensis Radix by a novel ensemble method with near-infrared spectroscopy","authors":"Xihui Bian , Yuxia Liu , Jiaqi Xie , Chunyan Zhao , Xiaoyao Tan , Weilu Tian","doi":"10.1016/j.jarmap.2025.100640","DOIUrl":"10.1016/j.jarmap.2025.100640","url":null,"abstract":"<div><div>The integrity and authenticity of Chinese medicinal materials are essential for ensuring the efficacy of medicines and safety of patients. <em>Angelicae Sinensis Radix</em> (ASR), a highly valued traditional Chinese medicine (TCM), is often substituted or mixed with lower-cost alternatives. In this study, a novel method was proposed for the quantification of adulterated ASR using near-infrared (NIR) spectroscopy combined with chemometrics. Binary and ternary adulterated ASR samples were prepared by mixing ASR with <em>Angelicae Pubescentis Radix</em> (APR) and <em>Chuanxiong Rhizoma</em> (CR), and their NIR spectra were measured in the range of 1000–1800 nm. Sample subsets were initially selected using Monte Carlo (MC) sampling, followed by the application of whale optimization algorithm (WOA) to select variables and establish an extreme learning machine (ELM) model, referred to as MC-WOA-ELM. The iteration number and percentage of training subsets for MC-WOA-ELM were optimized to further improve the predictive performance of model. The predictive ability of the proposed method was compared with partial least squares (PLS), ELM and WOA-ELM for both binary and ternary adulterated ASR samples. The results indicate that MC-WOA-ELM achieves the highest prediction accuracy. Therefore, the proposed method is rapid and intelligent for the quantification of adulterated ASR samples.</div></div>","PeriodicalId":15136,"journal":{"name":"Journal of Applied Research on Medicinal and Aromatic Plants","volume":"46 ","pages":"Article 100640"},"PeriodicalIF":3.8,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143935039","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-04-01DOI: 10.1016/j.jarmap.2025.100637
Farzaneh Mirzabayati, Amir Hossein Hamidian
Environmental pollution, particularly with heavy metals, is a critical issue in assessing the quality of medicinal plants. The concentration of heavy metals in these plants depends on factors such as growth environment, species, and processing methods. Contaminants absorbed by plants can enter the human body through consumption. This review examines the impact of heavy metals—cadmium (Cd), lead (Pb), arsenic (As), copper (Cu), chromium (Cr), zinc (Zn), and nickel (Ni)—on medicinal plants in Iran. It explores how environmental contamination affects plant quality and analyzes trends in measurement techniques and metal concentrations over time. Results show that Cd has the most significant negative impact on plant growth (63 %), followed by Pb (21 %), Cr (11 %), and Zn (5 %). Cd exceeded WHO limits in 47 % of samples, while Pb and Zn exceeded limits in 26 % and 20 %, respectively. Cd and Pb also significantly reduced germination rates. Contamination with Cd and Pb poses serious health risks, including kidney damage, bone disorders, cancer, neurological issues, and reproductive harm. Strict quality control, sustainable farming, and public awareness are essential to mitigate these risks. This study highlights the need for further research, particularly in industrial regions like Tehran, Khuzestan, Isfahan, and Fars, to ensure the safety of medicinal plants.
{"title":"Heavy metal pollution in Iranian medicinal plants, a review of sources, distribution, and health implications","authors":"Farzaneh Mirzabayati, Amir Hossein Hamidian","doi":"10.1016/j.jarmap.2025.100637","DOIUrl":"10.1016/j.jarmap.2025.100637","url":null,"abstract":"<div><div>Environmental pollution, particularly with heavy metals, is a critical issue in assessing the quality of medicinal plants. The concentration of heavy metals in these plants depends on factors such as growth environment, species, and processing methods. Contaminants absorbed by plants can enter the human body through consumption. This review examines the impact of heavy metals—cadmium (Cd), lead (Pb), arsenic (As), copper (Cu), chromium (Cr), zinc (Zn), and nickel (Ni)—on medicinal plants in Iran. It explores how environmental contamination affects plant quality and analyzes trends in measurement techniques and metal concentrations over time. Results show that Cd has the most significant negative impact on plant growth (63 %), followed by Pb (21 %), Cr (11 %), and Zn (5 %). Cd exceeded WHO limits in 47 % of samples, while Pb and Zn exceeded limits in 26 % and 20 %, respectively. Cd and Pb also significantly reduced germination rates. Contamination with Cd and Pb poses serious health risks, including kidney damage, bone disorders, cancer, neurological issues, and reproductive harm. Strict quality control, sustainable farming, and public awareness are essential to mitigate these risks. This study highlights the need for further research, particularly in industrial regions like Tehran, Khuzestan, Isfahan, and Fars, to ensure the safety of medicinal plants.</div></div>","PeriodicalId":15136,"journal":{"name":"Journal of Applied Research on Medicinal and Aromatic Plants","volume":"46 ","pages":"Article 100637"},"PeriodicalIF":3.8,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143923321","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-04-01DOI: 10.1016/j.jarmap.2025.100639
Zhong-hao Zhang , Zhong-hua Zhao , Tian-yi Cao , Cai-rong Yang , Zi-zhong Tang , Shi-ling Feng , Shu Yuan , Sheng-lin Hu , Ming Yuan
Ginger leaves are often discarded in agriculture, exacerbating continuous cropping obstacles. Polysaccharide, due to their low toxicity and diverse bioactivities, have garnered considerable interest. To isolate ginger leaf polysaccharide (GLP), we compared three extraction methods: hot water extraction, ultrasonic-assisted hot water extraction, and ultrasonic-assisted aqueous two-phase system extraction. Response surface methodology optimization revealed ultrasonic-assisted hot water extraction as the most efficient method, achieving a yield of 9.31 % under a liquid-solid ratio of 30 mL/g and an ultrasonic power of 150 W at 65 ℃ for 22 min. Polysaccharide from different extraction methods showed difference in monosaccharide composition and molecular weight. The ginger leaf polysaccharide demonstrated strong antioxidant in vitro and hypolipidemic activities in Caenorhabditis elegans, and that from the ultrasonic-assisted hot water method showed the highest bioactivity. These findings highlight ultrasonic-assisted hot water extraction as the optimal method for efficient extraction of GLP and discover GLP’s potential for pharmaceutical and functional food applications.
{"title":"Comparative analysis of three extraction methods for ginger leaf polysaccharide: Chemical composition, antioxidant activity, and hypolipidemic effects","authors":"Zhong-hao Zhang , Zhong-hua Zhao , Tian-yi Cao , Cai-rong Yang , Zi-zhong Tang , Shi-ling Feng , Shu Yuan , Sheng-lin Hu , Ming Yuan","doi":"10.1016/j.jarmap.2025.100639","DOIUrl":"10.1016/j.jarmap.2025.100639","url":null,"abstract":"<div><div>Ginger leaves are often discarded in agriculture, exacerbating continuous cropping obstacles. Polysaccharide, due to their low toxicity and diverse bioactivities, have garnered considerable interest. To isolate ginger leaf polysaccharide (GLP), we compared three extraction methods: hot water extraction, ultrasonic-assisted hot water extraction, and ultrasonic-assisted aqueous two-phase system extraction. Response surface methodology optimization revealed ultrasonic-assisted hot water extraction as the most efficient method, achieving a yield of 9.31 % under a liquid-solid ratio of 30 mL/g and an ultrasonic power of 150 W at 65 ℃ for 22 min. Polysaccharide from different extraction methods showed difference in monosaccharide composition and molecular weight. The ginger leaf polysaccharide demonstrated strong antioxidant <em>in vitro</em> and hypolipidemic activities in <em>Caenorhabditis elegans</em>, and that from the ultrasonic-assisted hot water method showed the highest bioactivity. These findings highlight ultrasonic-assisted hot water extraction as the optimal method for efficient extraction of GLP and discover GLP’s potential for pharmaceutical and functional food applications.</div></div>","PeriodicalId":15136,"journal":{"name":"Journal of Applied Research on Medicinal and Aromatic Plants","volume":"46 ","pages":"Article 100639"},"PeriodicalIF":3.8,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143918449","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Drought stress represents a significant constraint on the productivity of crops globally. The application of plant growth regulators, particularly brassinosteroids (BRs), has demonstrated potential in enhancing plant tolerance to drought conditions. This study aimed to examine the effects of exogenous BR application on the photosynthetic and morphological characteristics, as well as the concentrations of Delta-9-Tetrahydrocannabinol (THC), in Cannabis sativa subjected to drought stress. A split-plot experimental design was implemented over two growing seasons, with the primary factor being the planting date (May 20, June 5, June 20) and the secondary factor being the irrigation level (80–100 %, 50–60 %, and 30–40 % of field capacity). Exogenous BR treatments (0, 200, and 400 μg/L) were applied as the tertiary factor. The results indicated that drought stress significantly diminished photosynthetic efficiency, plant growth, and THC concentrations in C. sativa. Conversely, the exogenous application of BRs alleviated the adverse effects of drought, leading to enhanced photosynthetic performance, improved morphological traits, and increased THC levels compared to drought-stressed plants that did not receive BR treatment. These findings suggest that the exogenous application of BRs may serve as a promising strategy to improve drought tolerance and the medicinal properties of C. sativa. The study offers valuable insights into the role of plant growth regulators in modulating the physiological and biochemical responses of this important medicinal plant under conditions of water limitation.
{"title":"Does the exogenous application of brassinosteroids affect the photosynthetic, morphological characteristics, and THC concentrations of Cannabis sativa L. under drought stress?","authors":"Shiva Barooti , Mohsen Edalat , Mostafa Oveisi , Seyed Abdolreza Kazemeini , Ruhollah Naderi","doi":"10.1016/j.jarmap.2025.100635","DOIUrl":"10.1016/j.jarmap.2025.100635","url":null,"abstract":"<div><div>Drought stress represents a significant constraint on the productivity of crops globally. The application of plant growth regulators, particularly brassinosteroids (BRs), has demonstrated potential in enhancing plant tolerance to drought conditions. This study aimed to examine the effects of exogenous BR application on the photosynthetic and morphological characteristics, as well as the concentrations of Delta-9-Tetrahydrocannabinol (THC), in <em>Cannabis sativa</em> subjected to drought stress. A split-plot experimental design was implemented over two growing seasons, with the primary factor being the planting date (May 20, June 5, June 20) and the secondary factor being the irrigation level (80–100 %, 50–60 %, and 30–40 % of field capacity). Exogenous BR treatments (0, 200, and 400 μg/L) were applied as the tertiary factor. The results indicated that drought stress significantly diminished photosynthetic efficiency, plant growth, and THC concentrations in <em>C. sativa</em>. Conversely, the exogenous application of BRs alleviated the adverse effects of drought, leading to enhanced photosynthetic performance, improved morphological traits, and increased THC levels compared to drought-stressed plants that did not receive BR treatment. These findings suggest that the exogenous application of BRs may serve as a promising strategy to improve drought tolerance and the medicinal properties of <em>C. sativa</em>. The study offers valuable insights into the role of plant growth regulators in modulating the physiological and biochemical responses of this important medicinal plant under conditions of water limitation.</div></div>","PeriodicalId":15136,"journal":{"name":"Journal of Applied Research on Medicinal and Aromatic Plants","volume":"46 ","pages":"Article 100635"},"PeriodicalIF":3.8,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143918452","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-04-01DOI: 10.1016/j.jarmap.2025.100638
G. Mahendiran , Y.N. Venkatesh , Anna Jose , B. Manu , M. Ashajyothi , K. Sreedevi , S.N. Sushil
The lasoda weevil, Barioscapus cordiae (Marshall) is redescribed with digital images of habitus, morphometric details and images of diagnostic characters. The monotypic weevil genus Barioscapus Pajni and Kohli is easily recognised from other genera in the tribe by the presence of very closely related proxcoxae, antennae inserted at two-third length of rostrum from base, scape remote from eyes, prosternum without a depression before coxae, and exposed pygidium. The first DNA barcode was obtained and reported for molecular identification using cytochrome oxidase I (COI). The estimated total percentage of damage to fruits and seeds caused by B. cordiae to Cordia myxa was 82.07–88.08 %.
{"title":"Morpho-molecular identification of Barioscapus cordiae (Marshall) (Coleoptera: Curculionidae): A serious pest of Cordia myxa L.","authors":"G. Mahendiran , Y.N. Venkatesh , Anna Jose , B. Manu , M. Ashajyothi , K. Sreedevi , S.N. Sushil","doi":"10.1016/j.jarmap.2025.100638","DOIUrl":"10.1016/j.jarmap.2025.100638","url":null,"abstract":"<div><div>The lasoda weevil, <em>Barioscapus cordiae</em> (Marshall) is redescribed with digital images of habitus, morphometric details and images of diagnostic characters. The monotypic weevil genus <em>Barioscapus</em> Pajni and Kohli is easily recognised from other genera in the tribe by the presence of very closely related proxcoxae, antennae inserted at two-third length of rostrum from base, scape remote from eyes, prosternum without a depression before coxae, and exposed pygidium. The first DNA barcode was obtained and reported for molecular identification using cytochrome oxidase I (COI). The estimated total percentage of damage to fruits and seeds caused by <em>B</em>. <em>cordiae</em> to <em>Cordia myxa</em> was 82.07–88.08 %.</div></div>","PeriodicalId":15136,"journal":{"name":"Journal of Applied Research on Medicinal and Aromatic Plants","volume":"46 ","pages":"Article 100638"},"PeriodicalIF":3.8,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143918453","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}