Pub Date : 2025-09-01Epub Date: 2025-08-18DOI: 10.1016/j.jarmap.2025.100653
Alban Ibraliu , Drini Imami , Xavier Simonnet , Christoph Carlen , Andrea Pieroni , Andrea Primavera , Zamir Libohova , Sokrat Sinaj
Albania's rich biodiversity and diverse climatic conditions make it an ideal country for the collection and cultivation of medicinal and aromatic plants (MAPs). With over 400 indigenous MAP species, this sector plays a significant role in the country's economy, generating more than 50 million Euros annually and accounting for about 25 % of total agricultural exports. Moreover, the sector provides employment and income for thousands of rural households. This study examines the MAPs sector trends and patterns in Albania, highlighting strengths, weaknesses, threats and opportunities. Despite the global rise in demand for MAP products, driven by technological advancements and changing consumer attitudes, the Albanian MAPs sector faces challenges related to standards, processing capabilities and market access. Through a combination of qualitative interviews with key stakeholders and analysis, this study offers a comprehensive overview of the sector's current development, identifies critical issues, and provides recommendations for economic and political actors aimed at strengthening the value chain and market competitiveness of Albanian MAPs.
{"title":"Assessment of the medicinal and aromatic plants value chain in Albania: Challenges and development potentials","authors":"Alban Ibraliu , Drini Imami , Xavier Simonnet , Christoph Carlen , Andrea Pieroni , Andrea Primavera , Zamir Libohova , Sokrat Sinaj","doi":"10.1016/j.jarmap.2025.100653","DOIUrl":"10.1016/j.jarmap.2025.100653","url":null,"abstract":"<div><div>Albania's rich biodiversity and diverse climatic conditions make it an ideal country for the collection and cultivation of medicinal and aromatic plants (MAPs). With over 400 indigenous MAP species, this sector plays a significant role in the country's economy, generating more than 50 million Euros annually and accounting for about 25 % of total agricultural exports. Moreover, the sector provides employment and income for thousands of rural households. This study examines the MAPs sector trends and patterns in Albania, highlighting strengths, weaknesses, threats and opportunities. Despite the global rise in demand for MAP products, driven by technological advancements and changing consumer attitudes, the Albanian MAPs sector faces challenges related to standards, processing capabilities and market access. Through a combination of qualitative interviews with key stakeholders and analysis, this study offers a comprehensive overview of the sector's current development, identifies critical issues, and provides recommendations for economic and political actors aimed at strengthening the value chain and market competitiveness of Albanian MAPs.</div></div>","PeriodicalId":15136,"journal":{"name":"Journal of Applied Research on Medicinal and Aromatic Plants","volume":"48 ","pages":"Article 100653"},"PeriodicalIF":3.6,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144880126","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-09-01Epub Date: 2025-09-10DOI: 10.1016/j.jarmap.2025.100658
K.V. Ashwini, S. Gazala Parveen, Channayya Hiremath
Cultivar recommendation for commercial scale cultivation involves evaluating several advanced breeding lines across multiple locations and years to identify the most promising and stable line(s). Theoretically, it is suggested that cultivars’ single-location multiple-year performance data has higher predictive power of their future performance than single year data. In the present study, we attempted to examine this hypothesis in 13 lemongrass clones evaluated across four years (2018–2021) at CSIR-Central Institute of Medicinal and Aromatic Plants Research Centre, Bengaluru, India for herbage and essential oil yield using the statistical predictors viz., best linear unbiased predictors (BLUP) and yield relative to environment maximum (YREM). Besides, we also identified stable and high herbage and essential oil yielding lemongrass clones using AMMI based stability parameters, mean BLUP and YREMs. The predictive power of single-year vs multi-year data was assessed by comparing the correlation co-efficients of the lemongrass clones’ current year’s performance with preceding 1-, 2- and 3- years’ performance. The results of our study indicated that the multi-year and single-year performance data were adequate to predict the lemongrass’ herbage and essential oil yield, respectively for future years’ performance and to identify superior clones. The clone B3 3–1 exhibited superior herbage and essential oil yield based on mean BLUP and YREM as measures of clones’ performance, besides exhibiting excellent stability as indicated by AMMI-based stability estimates. Further, evaluation of the clone 5 (B2 6–4) for stable herbage and essential oil yield performance across multi-locations shall aid in identifying a cultivar suitable for commercial cultivation.
商业规模栽培的品种推荐包括在多个地点和年份评估几个先进的育种品系，以确定最有前途和最稳定的品系。从理论上讲，品种单地点多年产量数据对其未来产量的预测能力高于单年份数据。在本研究中，我们试图在印度班加罗尔csir -中央药用和芳香植物研究中心（CSIR-Central Institute of Medicinal and Aromatic Plants Research Centre）对13个柠檬草无性系进行四年（2018-2021）评估，利用统计预测因子，即最佳线性无偏预测因子（BLUP）和相对于环境最大值（YREM）的产量，来检验这一假设。此外，我们还利用基于AMMI的稳定性参数、平均BLUP和YREMs，鉴定出稳定高产的柠檬草无性系。通过比较柠檬草无性系当年表现与前1年、2年和3年表现的相关系数，对单年和多年数据的预测能力进行了评估。本研究结果表明，多年和单年的产量数据足以预测香茅的牧草产量和精油产量，分别用于预测未来年份的产量和鉴定优质无性系。基于平均BLUP和YREM的无性系B3 - 1表现出优异的牧草和精油产量，此外，基于ammi的稳定性估计表明，无性系B3 - 1表现出优异的稳定性。此外，对5号无性系（B2 6-4）在多个地点的稳定牧草和精油产量性能进行评估，将有助于确定适合商业种植的品种。
{"title":"Comparative predictive power of single-year vs. multi-year BLUPs and YREMs for herbage and essential oil yield in lemongrass (Cymbopogon flexuosus Stapf)","authors":"K.V. Ashwini, S. Gazala Parveen, Channayya Hiremath","doi":"10.1016/j.jarmap.2025.100658","DOIUrl":"10.1016/j.jarmap.2025.100658","url":null,"abstract":"<div><div>Cultivar recommendation for commercial scale cultivation involves evaluating several advanced breeding lines across multiple locations and years to identify the most promising and stable line(s). Theoretically, it is suggested that cultivars’ single-location multiple-year performance data has higher predictive power of their future performance than single year data. In the present study, we attempted to examine this hypothesis in 13 lemongrass clones evaluated across four years (2018–2021) at CSIR-Central Institute of Medicinal and Aromatic Plants Research Centre, Bengaluru, India for herbage and essential oil yield using the statistical predictors <em>viz.,</em> best linear unbiased predictors (BLUP) and yield relative to environment maximum (YREM). Besides, we also identified stable and high herbage and essential oil yielding lemongrass clones using AMMI based stability parameters, mean BLUP and YREMs. The predictive power of single-year <em>vs</em> multi-year data was assessed by comparing the correlation co-efficients of the lemongrass clones’ current year’s performance with preceding 1-, 2- and 3- years’ performance. The results of our study indicated that the multi-year and single-year performance data were adequate to predict the lemongrass’ herbage and essential oil yield, respectively for future years’ performance and to identify superior clones. The clone B3 3–1 exhibited superior herbage and essential oil yield based on mean BLUP and YREM as measures of clones’ performance, besides exhibiting excellent stability as indicated by AMMI-based stability estimates. Further, evaluation of the clone 5 (B2 6–4) for stable herbage and essential oil yield performance across multi-locations shall aid in identifying a cultivar suitable for commercial cultivation.</div></div>","PeriodicalId":15136,"journal":{"name":"Journal of Applied Research on Medicinal and Aromatic Plants","volume":"48 ","pages":"Article 100658"},"PeriodicalIF":3.6,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145044523","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-09-01Epub Date: 2025-07-29DOI: 10.1016/j.jarmap.2025.100649
Sara Ahmadi Shokooh, Masoud Fereidoni
Plant-derived exosome-like nanoparticles (PELNPs) represent promising bioactive agents, with ginger-derived exosome-like nanoparticles (GELNPs) exhibiting notable therapeutic potential due to their anti-inflammatory, antioxidant, and neuroprotective properties. While polyethylene glycol (PEG)-based extraction offers a cost-effective alternative to ultracentrifugation for PELNP isolation, methodological refinements are needed to enhance yield, stability, and reproducibility. This study systematically optimizes GELNP isolation by integrating PEG6000 precipitation with pH adjustment and 0.45 µm filtration, building upon existing ginger nanovesicle protocols. We present the first comparative evaluation of pH modulation versus filtration, demonstrating distinct trade-offs: pH-adjusted GELNPs achieved smaller size, whereas filtered GELNPs exhibited superior colloidal stability and lower polydispersity index (PDI). Furthermore, we identify previously unreported seasonal variations in GELNP properties, with July-derived nanoparticles displaying enhanced stability and reduced size compared to those isolated in other months. Comprehensive physicochemical characterization—including transmission electron microscopy (TEM), atomic force microscopy (AFM), particle size analysis (PSA), zeta potential analysis, and Fourier-transform infrared spectroscopy (FT-IR)—confirmed differences in morphology, size distribution, surface charge, and biomolecular composition. These findings highlight the necessity for seasonally adjusted, optimized isolation protocols to ensure batch-to-batch consistency and maximize therapeutic efficacy, particularly for drug delivery applications. By addressing critical methodological gaps and introducing temporal considerations, this work establishes a framework for scalable, high-quality GELNP production.
{"title":"Optimization of ginger-derived exosome-like nanoparticle isolation: Synergistic effects of polyethylene glycol, filtration, and pH","authors":"Sara Ahmadi Shokooh, Masoud Fereidoni","doi":"10.1016/j.jarmap.2025.100649","DOIUrl":"10.1016/j.jarmap.2025.100649","url":null,"abstract":"<div><div>Plant-derived exosome-like nanoparticles (PELNPs) represent promising bioactive agents, with ginger-derived exosome-like nanoparticles (GELNPs) exhibiting notable therapeutic potential due to their anti-inflammatory, antioxidant, and neuroprotective properties. While polyethylene glycol (PEG)-based extraction offers a cost-effective alternative to ultracentrifugation for PELNP isolation, methodological refinements are needed to enhance yield, stability, and reproducibility. This study systematically optimizes GELNP isolation by integrating PEG6000 precipitation with pH adjustment and 0.45 µm filtration, building upon existing ginger nanovesicle protocols. We present the first comparative evaluation of pH modulation versus filtration, demonstrating distinct trade-offs: pH-adjusted GELNPs achieved smaller size, whereas filtered GELNPs exhibited superior colloidal stability and lower polydispersity index (PDI). Furthermore, we identify previously unreported seasonal variations in GELNP properties, with July-derived nanoparticles displaying enhanced stability and reduced size compared to those isolated in other months. Comprehensive physicochemical characterization—including transmission electron microscopy (TEM), atomic force microscopy (AFM), particle size analysis (PSA), zeta potential analysis, and Fourier-transform infrared spectroscopy (FT-IR)—confirmed differences in morphology, size distribution, surface charge, and biomolecular composition. These findings highlight the necessity for seasonally adjusted, optimized isolation protocols to ensure batch-to-batch consistency and maximize therapeutic efficacy, particularly for drug delivery applications. By addressing critical methodological gaps and introducing temporal considerations, this work establishes a framework for scalable, high-quality GELNP production.</div></div>","PeriodicalId":15136,"journal":{"name":"Journal of Applied Research on Medicinal and Aromatic Plants","volume":"48 ","pages":"Article 100649"},"PeriodicalIF":3.6,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144771136","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-05-01Epub Date: 2025-06-14DOI: 10.1016/j.jarmap.2025.100643
Yanan Cao , Menghao Wang , Jiamei Li , Ying Yang , Hao Wang , Yanyan Liu , Hongwei Wang , Risong Na
Aralia section Dimorphanthus represents the largest section in Araliaceae and has been employed in East Asian traditional medicine for centuries. However, the morphological complexity and the prevalence of hybridization/introgression posed a great challenge for accurate species identification, thereby hindering the development, conservation and application of these medicinal plants. In this study, we employed sequences of hundreds of nuclear loci and the complete plastomes to reconstruct the phylogenetic relationships, and evaluated the effectiveness of 12 distinct barcode systems for distinguishing Chinese species of Aralia sect. Dimorphanthus. We found that plastome length, gene content, and order of these species were highly conserved, while the inverted repeat regions showed variability between temperate and tropical/subtropical lineages, providing novel markers for geographical traceability. Phylogenetic relationships constructed based on the genome-scale data confirmed that Chinese Aralia sect. Dimorphanthus was monophyletic and comprised three well-supported lineages. Incomplete lineage sorting and hybridization were identified as the main factors contributing to the phylogenetic conflicts among trees. Species discrimination analysis revealed that super-barcoding strategies integrating complete plastomes and nrDNA sequences, achieved a 92 % species identification accuracy among the 14 studied species of Aralia sect. Dimorphanthus, outperforming standard barcodes. While this system resolved most taxonomic ambiguities, the inclusion of universal nuclear markers is crucial to resolve ambiguities in highly variable taxa like A. elata. These advancements directly support industrial needs for reliable species authentication and biodiversity conservation in pharmacologically important species.
{"title":"Resolving taxonomic complexities in medicinal Aralia: Genome-scale phylogeny and super-barcoding advance species identification and conservation","authors":"Yanan Cao , Menghao Wang , Jiamei Li , Ying Yang , Hao Wang , Yanyan Liu , Hongwei Wang , Risong Na","doi":"10.1016/j.jarmap.2025.100643","DOIUrl":"10.1016/j.jarmap.2025.100643","url":null,"abstract":"<div><div><em>Aralia</em> section <em>Dimorphanthus</em> represents the largest section in Araliaceae and has been employed in East Asian traditional medicine for centuries. However, the morphological complexity and the prevalence of hybridization/introgression posed a great challenge for accurate species identification, thereby hindering the development, conservation and application of these medicinal plants. In this study, we employed sequences of hundreds of nuclear loci and the complete plastomes to reconstruct the phylogenetic relationships, and evaluated the effectiveness of 12 distinct barcode systems for distinguishing Chinese species of <em>Aralia</em> sect. <em>Dimorphanthus</em>. We found that plastome length, gene content, and order of these species were highly conserved, while the inverted repeat regions showed variability between temperate and tropical/subtropical lineages, providing novel markers for geographical traceability. Phylogenetic relationships constructed based on the genome-scale data confirmed that Chinese <em>Aralia</em> sect. <em>Dimorphanthus</em> was monophyletic and comprised three well-supported lineages. Incomplete lineage sorting and hybridization were identified as the main factors contributing to the phylogenetic conflicts among trees. Species discrimination analysis revealed that super-barcoding strategies integrating complete plastomes and nrDNA sequences, achieved a 92 % species identification accuracy among the 14 studied species of <em>Aralia</em> sect. <em>Dimorphanthus</em>, outperforming standard barcodes. While this system resolved most taxonomic ambiguities, the inclusion of universal nuclear markers is crucial to resolve ambiguities in highly variable taxa like <em>A. elata</em>. These advancements directly support industrial needs for reliable species authentication and biodiversity conservation in pharmacologically important species.</div></div>","PeriodicalId":15136,"journal":{"name":"Journal of Applied Research on Medicinal and Aromatic Plants","volume":"47 ","pages":"Article 100643"},"PeriodicalIF":3.8,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144306654","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-05-01Epub Date: 2025-06-18DOI: 10.1016/j.jarmap.2025.100642
Haotian Zhong , Lina Chen , Lei Chen , Xiao Huang , Ling Gong , Juan Hu , Yuling Zeng , Kun Yu , Lin Sen
In this study, we aimed to develop SSR molecular markers for assessing the population structure and genetic diversity of Atractylodes lancea (AL) and Atractylodes chinensis (AC), with a focus on their volatile compounds. By utilizing transcriptome data from AL, we successfully developed 29 pairs of highly polymorphic SSR primers, generating 264 distinct alleles. Cluster analysis revealed three distinct populations: Mao Mountain, Dabie Mountain, and samples from other locations and primers with high discriminatory power were selected. Additionally, a representative core subset of 106 samples was selected from the original germplasm, capable of representing over 99 % of the genetic variation. This core subset provides an efficient resource for future studies on population genetics and trait association. The Support Vector Machine (SVM)-based method achieved an accuracy of 98.8 % in distinguishing Atractylodis Rhizoma (AR) varieties. GC-MS screening identified 24 potential differential metabolites between the two species, with correlation analysis showing significant association. This study successfully developed SSR molecular markers for analyzing genetic diversity in AL and AC and established a reliable method for identifying AR medicinal varieties. These findings provide a solid foundation for future quality control of AR and the selection of superior germplasm.
{"title":"Effective SSR markers for assessing population structure, genetic diversity, and volatile compounds in Atractylodis Rhizoma","authors":"Haotian Zhong , Lina Chen , Lei Chen , Xiao Huang , Ling Gong , Juan Hu , Yuling Zeng , Kun Yu , Lin Sen","doi":"10.1016/j.jarmap.2025.100642","DOIUrl":"10.1016/j.jarmap.2025.100642","url":null,"abstract":"<div><div>In this study, we aimed to develop SSR molecular markers for assessing the population structure and genetic diversity of <em>Atractylodes lancea</em> (AL) and <em>Atractylodes chinensis</em> (AC), with a focus on their volatile compounds. By utilizing transcriptome data from AL, we successfully developed 29 pairs of highly polymorphic SSR primers, generating 264 distinct alleles. Cluster analysis revealed three distinct populations: Mao Mountain, Dabie Mountain, and samples from other locations and primers with high discriminatory power were selected. Additionally, a representative core subset of 106 samples was selected from the original germplasm, capable of representing over 99 % of the genetic variation. This core subset provides an efficient resource for future studies on population genetics and trait association. The Support Vector Machine (SVM)-based method achieved an accuracy of 98.8 % in distinguishing Atractylodis Rhizoma (AR) varieties. GC-MS screening identified 24 potential differential metabolites between the two species, with correlation analysis showing significant association. This study successfully developed SSR molecular markers for analyzing genetic diversity in AL and AC and established a reliable method for identifying AR medicinal varieties. These findings provide a solid foundation for future quality control of AR and the selection of superior germplasm.</div></div>","PeriodicalId":15136,"journal":{"name":"Journal of Applied Research on Medicinal and Aromatic Plants","volume":"47 ","pages":"Article 100642"},"PeriodicalIF":3.8,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144472219","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-05-01Epub Date: 2025-06-03DOI: 10.1016/j.jarmap.2025.100641
Ramon G. Paschoal , Marianne L. Higino , Ana Paula G. Alves , André Luiz F. Sampaio , Dulcineia F. Teixeira , Leonardo Lucchetti , Marisa F. Mendes
Different plants have been used, around the world, in the treatment of primary diseases as an alternative or popular medicine. In Brazil, the Health Minister introduced a national politic for the phytotherapy use in the Single Health System (SUS). Due to that, Bidenspilosa L. (Asteraceae) was chosen because it is one of the medicinal plants present in the Single Health System list, called RENISUS. Bidens pilosa L. is a popular plant used in the treatment of different diseases (malaria, cancer tumors, fever, diabetes, infections, inflammations, angina, etc.) and is widely disseminated throughout the tropical and subtropical regions of the world. The biological activities of the plant are attributed to the presence of flavonoids, polyacetylenes and terpenes. Therefore, this study evaluated the biological activities of the oil extraction using supercritical CO2 at different pressure and temperature conditions, due to the scarcity of studies comparing supercritical fluid extraction to conventional techniques (Soxhlet and maceration). The experimental procedure used 8 g of previously dried and milled leaves at different conditions according to an experimental design (150 bar - 60 °C; 179 bar - 46 °C; 179 bar - 74 °C; 250 bar - 40 °C; 250 bar - 80 °C; 321 bar - 46 °C; 321 bar - 74 °C, 350 bar - 60 °C, 250 bar - 60 °C). According to the results, the best yield was 0.082 % (6.6 mg) at 321 bar and 74 °C. The analyses of the experiments done at 321 bar at 74 °C and 250 bar at 40 °C, showed ultraviolet spectra characteristic of terpenes. The extracts analyzed showed promising inhibition in the two tumor cell lines: melanoma, SK-MEL-28 (68 %) and leukemia K562 (90 %), both at a concentration of 150 μg/mL. The results suggested the importance of the bioactive components from Bidens pilosa to contribute to a new anticancer drug using supercritical technology.
在世界各地，不同的植物已被用作治疗原发性疾病的替代药物或流行药物。在巴西，卫生部长提出了在单一卫生系统（SUS）中使用植物疗法的国家政策。因此，选择Bidenspilosa L. （Asteraceae）是因为它是单一卫生系统清单（称为RENISUS）中存在的药用植物之一。Bidens pilosa L.是一种流行的植物，用于治疗不同的疾病（疟疾、癌症肿瘤、发烧、糖尿病、感染、炎症、心绞痛等），广泛分布在世界的热带和亚热带地区。该植物的生物活性归因于黄酮类化合物、聚乙炔和萜烯的存在。因此，由于缺乏将超临界流体萃取与常规技术（索氏和浸渍）进行比较的研究，因此，本研究评估了超临界CO2在不同压力和温度条件下的生物活性。实验程序使用8 g先前干燥和研磨的叶子，根据实验设计(150 bar - 60°C；179 bar - 46°C；179 bar - 74°C；250 bar - 40℃；250 bar - 80℃；321 bar - 46°C；321 bar - 74°C, 350 bar - 60°C, 250 bar - 60°C)。结果表明，在321 bar、74℃条件下，最佳产率为0.082 %（6.6 mg）。在74°C下321 bar和40°C下250 bar进行的实验分析显示了萜烯的紫外光谱特征。所分析的提取物对两种肿瘤细胞系SK-MEL-28（68 %）和白血病K562（90 %）均有抑制作用，浓度均为150 μg/mL。研究结果表明，从毛缕草中提取的活性成分对利用超临界技术开发新型抗癌药物具有重要意义。
{"title":"Supercritical fluid extraction of Bidens pilosa L. oil: Kinetics evaluation and antitumor activity","authors":"Ramon G. Paschoal , Marianne L. Higino , Ana Paula G. Alves , André Luiz F. Sampaio , Dulcineia F. Teixeira , Leonardo Lucchetti , Marisa F. Mendes","doi":"10.1016/j.jarmap.2025.100641","DOIUrl":"10.1016/j.jarmap.2025.100641","url":null,"abstract":"<div><div>Different plants have been used, around the world, in the treatment of primary diseases as an alternative or popular medicine. In Brazil, the Health Minister introduced a national politic for the phytotherapy use in the Single Health System (SUS). Due to that, <em>Bidenspilosa</em> L. (Asteraceae) was chosen because it is one of the medicinal plants present in the Single Health System list, called RENISUS. <em>Bidens pilosa</em> L. is a popular plant used in the treatment of different diseases (malaria, cancer tumors, fever, diabetes, infections, inflammations, angina, etc.) and is widely disseminated throughout the tropical and subtropical regions of the world. The biological activities of the plant are attributed to the presence of flavonoids, polyacetylenes and terpenes. Therefore, this study evaluated the biological activities of the oil extraction using supercritical CO<sub>2</sub> at different pressure and temperature conditions, due to the scarcity of studies comparing supercritical fluid extraction to conventional techniques (Soxhlet and maceration). The experimental procedure used 8 g of previously dried and milled leaves at different conditions according to an experimental design (150 bar - 60 °C; 179 bar - 46 °C; 179 bar - 74 °C; 250 bar - 40 °C; 250 bar - 80 °C; 321 bar - 46 °C; 321 bar - 74 °C, 350 bar - 60 °C, 250 bar - 60 °C). According to the results, the best yield was 0.082 % (6.6 mg) at 321 bar and 74 °C. The analyses of the experiments done at 321 bar at 74 °C and 250 bar at 40 °C, showed ultraviolet spectra characteristic of terpenes. The extracts analyzed showed promising inhibition in the two tumor cell lines: melanoma, SK-MEL-28 (68 %) and leukemia K562 (90 %), both at a concentration of 150 μg/mL. The results suggested the importance of the bioactive components from <em>Bidens pilosa</em> to contribute to a new anticancer drug using supercritical technology.</div></div>","PeriodicalId":15136,"journal":{"name":"Journal of Applied Research on Medicinal and Aromatic Plants","volume":"47 ","pages":"Article 100641"},"PeriodicalIF":3.8,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144280186","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-05-01Epub Date: 2025-07-03DOI: 10.1016/j.jarmap.2025.100645
Mingyu Han , Qiang Hu , Yuanzhong Wang
The quality and safety of Herbal medicines (HMs) not only affect the development of the industry chain, but also the life safety rights and interests of patients. Traditional HMs supply chains suffer from opaque information and easy tampering, but blockchain intervention can significantly improve the security and traceability of information. The combination of blockchain and herb chain will be the future trend. This paper uses bibliometric statistics to analyze the practical application scenarios of blockchain and summarizes the challenges faced in HMs supply chain. It further explores the application potential of blockchain in the field of HMs and designs a traceability system for HMs based on blockchain technology. A large number of literature surveys found that the application scope of blockchain in HMs is relatively small, and comprehensive coverage of blockchain applications has a high coefficient of difficulty. This paper provides more possibilities for the application of blockchain in HMs, which will promote the development of a benign cycle of the HMs industry chain and protect the rights and interests of consumers.
{"title":"Blockchain technology in herbal medicines: Applications, trends, and prospects","authors":"Mingyu Han , Qiang Hu , Yuanzhong Wang","doi":"10.1016/j.jarmap.2025.100645","DOIUrl":"10.1016/j.jarmap.2025.100645","url":null,"abstract":"<div><div>The quality and safety of Herbal medicines (HMs) not only affect the development of the industry chain, but also the life safety rights and interests of patients. Traditional HMs supply chains suffer from opaque information and easy tampering, but blockchain intervention can significantly improve the security and traceability of information. The combination of blockchain and herb chain will be the future trend. This paper uses bibliometric statistics to analyze the practical application scenarios of blockchain and summarizes the challenges faced in HMs supply chain. It further explores the application potential of blockchain in the field of HMs and designs a traceability system for HMs based on blockchain technology. A large number of literature surveys found that the application scope of blockchain in HMs is relatively small, and comprehensive coverage of blockchain applications has a high coefficient of difficulty. This paper provides more possibilities for the application of blockchain in HMs, which will promote the development of a benign cycle of the HMs industry chain and protect the rights and interests of consumers.</div></div>","PeriodicalId":15136,"journal":{"name":"Journal of Applied Research on Medicinal and Aromatic Plants","volume":"47 ","pages":"Article 100645"},"PeriodicalIF":3.8,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144588674","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-05-01Epub Date: 2025-06-24DOI: 10.1016/j.jarmap.2025.100644
Qingying Zeng , Yang Zhao , Xiurong Wang , Meixuan Xie , Fuhua Liu , Xingfu Zhang
Gleditsia sinensis is a tree species with high economic value, but the pairing of male and female trees is often overlooked in its cultivation practices. The fruit quality of G. sinensis under natural pollination tends to be inconsistent, resulting in lower economic value. Therefore, understanding the xenia effects of G. sinensis on fruit and seed quality is crucial for improving their overall quality. In this study, The G. sinensis as the female parent and Gleditsia japonica var. delavayi, Gleditsia japonica, G. sinensis, and Gleditsia fera as male parents for hybridization. By observing and comparing the inflorescence characteristics of different male parents, as well as the fruit set rates, pod phenotypes, and seed yield under various pollination combinations, we found that artificial pollination significantly increased the yield of pods and seeds, and distinct xenia effects were observed in the fruits and seeds of G. sinensis. Through comprehensive evaluation, G. fera was identified as the optimal pollen source for interspecific hybridization with G. sinensis, significantly increasing fruit set rate, saponin content, and seed yield. Pollination with G. japonica resulted in a higher seed abortion rate and poorer seed quality, but significantly increased the amino acid and starch content in the pods. G. delavayi pollen is more inclined to produce high-quality seeds. These findings provide valuable insights for the selection of G. sinensis pollinating trees and the improvement of G. sinensis fruit quality.
Data availability
All data generated in this study are presented within the manuscript. Further information and requests could be directed to the lead contact, Prof. Yang Zhao ([email protected])
{"title":"Xenia effect enhance pod and seed quality in Gleditsia sinensis","authors":"Qingying Zeng , Yang Zhao , Xiurong Wang , Meixuan Xie , Fuhua Liu , Xingfu Zhang","doi":"10.1016/j.jarmap.2025.100644","DOIUrl":"10.1016/j.jarmap.2025.100644","url":null,"abstract":"<div><div><em>Gleditsia sinensis</em> is a tree species with high economic value, but the pairing of male and female trees is often overlooked in its cultivation practices. The fruit quality of <em>G. sinensis</em> under natural pollination tends to be inconsistent, resulting in lower economic value. Therefore, understanding the xenia effects of <em>G. sinensis</em> on fruit and seed quality is crucial for improving their overall quality. In this study, The <em>G. sinensis</em> as the female parent and <em>Gleditsia japonica var. delavayi</em>, <em>Gleditsia japonica</em>, <em>G. sinensis</em>, and <em>Gleditsia fera</em> as male parents for hybridization. By observing and comparing the inflorescence characteristics of different male parents, as well as the fruit set rates, pod phenotypes, and seed yield under various pollination combinations, we found that artificial pollination significantly increased the yield of pods and seeds, and distinct xenia effects were observed in the fruits and seeds of <em>G. sinensis</em>. Through comprehensive evaluation, <em>G. fera</em> was identified as the optimal pollen source for interspecific hybridization with <em>G. sinensis</em>, significantly increasing fruit set rate, saponin content, and seed yield. Pollination with <em>G. japonica</em> resulted in a higher seed abortion rate and poorer seed quality, but significantly increased the amino acid and starch content in the pods. <em>G. delavayi</em> pollen is more inclined to produce high-quality seeds. These findings provide valuable insights for the selection of <em>G. sinensis</em> pollinating trees and the improvement of <em>G. sinensis</em> fruit quality.</div></div><div><h3>Data availability</h3><div>All data generated in this study are presented within the manuscript. Further information and requests could be directed to the lead contact, Prof. Yang Zhao ([email protected])</div></div>","PeriodicalId":15136,"journal":{"name":"Journal of Applied Research on Medicinal and Aromatic Plants","volume":"47 ","pages":"Article 100644"},"PeriodicalIF":3.8,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144502269","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Clary sage (Salvia sclarea L.) is an aromatic and medicinal herb grown widely across Europe, Western Asia, Maghreb, the Mideast, and countries like India. The purpose of this study was to assess genetic diversity of clary sage genotypes from the Western Himalayas, aiming to support future breeding efforts. A total of 72 genotypes of clary sage from six distinct populations were examined using 49 simple sequence repeat markers. In total, 323 alleles were detected ranging from 1 to 16 with an average of 6.54 alleles per locus. Among these, 78.33 % were common alleles, 1.86 % were rare, and 19.81 % were frequent alleles. The results revealed an overall polymorphic information content of 93.88 %, with an average of 0.71 ranging from 0.00 to 0.93 at each marker locus, indicating high genetic variability among the studied material. The detected heterozygosity (0.52) was lower than expected heterozygosity (0.71), pointing a heterozygotes deficit populations. Analysis of variance showed considerable variability for biomass yield. Molecular analysis of variance (AMOVA) showed that genetic diversity was greater within individuals than among populations. Unweighted neighbour-joining, principal coordinate analysis, and population structure showed that the genotypes from different geographic regions and various biomass yield groups were predominantly admixed across clusters. The cluster-III represented the Bharmour population was the highest biomass performer group. The observed diversity patterns will facilitate plant breeders to make choices for breeding programs aiming for higher biomass yield in this plant.
{"title":"Characterizing trait-specific populations and genetic diversity patterns in clary sage (Salvia sclarea L.) using SSR markers","authors":"Niketa Yadav , Mohar Singh , Asmita Saini , Pratibha Pandey , Garima Thakur , Ramesh Chauhan , Satbeer Singh , Sanatsujat Singh","doi":"10.1016/j.jarmap.2025.100646","DOIUrl":"10.1016/j.jarmap.2025.100646","url":null,"abstract":"<div><div>Clary sage (<em>Salvia sclarea</em> L.) is an aromatic and medicinal herb grown widely across Europe, Western Asia, Maghreb, the Mideast, and countries like India. The purpose of this study was to assess genetic diversity of clary sage genotypes from the Western Himalayas, aiming to support future breeding efforts. A total of 72 genotypes of clary sage from six distinct populations were examined using 49 simple sequence repeat markers. In total, 323 alleles were detected ranging from 1 to 16 with an average of 6.54 alleles per locus. Among these, 78.33 % were common alleles, 1.86 % were rare, and 19.81 % were frequent alleles. The results revealed an overall polymorphic information content of 93.88 %, with an average of 0.71 ranging from 0.00 to 0.93 at each marker locus, indicating high genetic variability among the studied material. The detected heterozygosity (0.52) was lower than expected heterozygosity (0.71), pointing a heterozygotes deficit populations. Analysis of variance showed considerable variability for biomass yield. Molecular analysis of variance (AMOVA) showed that genetic diversity was greater within individuals than among populations. Unweighted neighbour-joining, principal coordinate analysis, and population structure showed that the genotypes from different geographic regions and various biomass yield groups were predominantly admixed across clusters. The cluster-III represented the Bharmour population was the highest biomass performer group. The observed diversity patterns will facilitate plant breeders to make choices for breeding programs aiming for higher biomass yield in this plant.</div></div>","PeriodicalId":15136,"journal":{"name":"Journal of Applied Research on Medicinal and Aromatic Plants","volume":"47 ","pages":"Article 100646"},"PeriodicalIF":3.8,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144614605","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-04-01Epub Date: 2025-03-08DOI: 10.1016/j.jarmap.2025.100633
Zhilong He , Zhao Yang , Cui Dong , Shanshan Yan , Jing Lin , Hexigeduleng Bao , Fan Tang , Leilei Zhang , Cheng Zhu , Yan Hu , Yanfei Ding
Dendrobium officinale is widely used as a precious traditional medicinal plant and health food in China. However, because of its high nutritional and medicinal value, it is often targeted for adulteration or mislabeling. This work aims to exploit DNA barcoding combined with high-resolution melting analysis to differentiate Dendrobium officinale（D. officinale）species from non-D. officinale species and to further identify its botanical origin. The internal transcribed spacer 2 (ITS2) was targeted as a candidate barcode for Dendrobium species identification. High resolution melting（HRM）method allowed differentiating the six Dendrobium species in three clusters with 95 % confidence interval, which is consistent with the sequencing analysis. ITS2 sequencing results implied that 43 % of commercial Dendrobium products may be mislabeled. Thus, ITS2 DNA barcoding with HRM assay could be used as a simple, specific and cost-effective tool to identify different Dendrobium species and commercial Dendrobium products, and to authenticate the origin of D. officinale.
{"title":"Identification of Dendrobium officinale species and authentication of commercial products by DNA barcoding and high-resolution melting analysis","authors":"Zhilong He , Zhao Yang , Cui Dong , Shanshan Yan , Jing Lin , Hexigeduleng Bao , Fan Tang , Leilei Zhang , Cheng Zhu , Yan Hu , Yanfei Ding","doi":"10.1016/j.jarmap.2025.100633","DOIUrl":"10.1016/j.jarmap.2025.100633","url":null,"abstract":"<div><div><em>Dendrobium officinale</em> is widely used as a precious traditional medicinal plant and health food in China. However, because of its high nutritional and medicinal value, it is often targeted for adulteration or mislabeling. This work aims to exploit DNA barcoding combined with high-resolution melting analysis to differentiate <em>Dendrobium officinale</em><strong>（</strong><em>D. officinale</em>）species from non-<em>D. officinale</em> species and to further identify its botanical origin. The internal transcribed spacer 2 (ITS2) was targeted as a candidate barcode for <em>Dendrobium</em> species identification. High resolution melting（HRM）method allowed differentiating the six <em>Dendrobium</em> species in three clusters with 95 % confidence interval, which is consistent with the sequencing analysis. ITS2 sequencing results implied that 43 % of commercial <em>Dendrobium</em> products may be mislabeled. Thus, ITS2 DNA barcoding with HRM assay could be used as a simple, specific and cost-effective tool to identify different <em>Dendrobium</em> species and commercial <em>Dendrobium</em> products, and to authenticate the origin of <em>D. officinale.</em></div></div>","PeriodicalId":15136,"journal":{"name":"Journal of Applied Research on Medicinal and Aromatic Plants","volume":"46 ","pages":"Article 100633"},"PeriodicalIF":3.8,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143918451","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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