Journal of Applied Research on Medicinal and Aromatic Plants最新文献_第6页

Optimization of ginger-derived exosome-like nanoparticle isolation: Synergistic effects of polyethylene glycol, filtration, and pH 姜源外泌体样纳米颗粒分离的优化：聚乙二醇、过滤和pH的协同效应

IF 3.6 2区农林科学 Q1 PLANT SCIENCES

Journal of Applied Research on Medicinal and Aromatic Plants

Pub Date : 2025-07-29 DOI: 10.1016/j.jarmap.2025.100649

Sara Ahmadi Shokooh, Masoud Fereidoni

Plant-derived exosome-like nanoparticles (PELNPs) represent promising bioactive agents, with ginger-derived exosome-like nanoparticles (GELNPs) exhibiting notable therapeutic potential due to their anti-inflammatory, antioxidant, and neuroprotective properties. While polyethylene glycol (PEG)-based extraction offers a cost-effective alternative to ultracentrifugation for PELNP isolation, methodological refinements are needed to enhance yield, stability, and reproducibility. This study systematically optimizes GELNP isolation by integrating PEG6000 precipitation with pH adjustment and 0.45 µm filtration, building upon existing ginger nanovesicle protocols. We present the first comparative evaluation of pH modulation versus filtration, demonstrating distinct trade-offs: pH-adjusted GELNPs achieved smaller size, whereas filtered GELNPs exhibited superior colloidal stability and lower polydispersity index (PDI). Furthermore, we identify previously unreported seasonal variations in GELNP properties, with July-derived nanoparticles displaying enhanced stability and reduced size compared to those isolated in other months. Comprehensive physicochemical characterization—including transmission electron microscopy (TEM), atomic force microscopy (AFM), particle size analysis (PSA), zeta potential analysis, and Fourier-transform infrared spectroscopy (FT-IR)—confirmed differences in morphology, size distribution, surface charge, and biomolecular composition. These findings highlight the necessity for seasonally adjusted, optimized isolation protocols to ensure batch-to-batch consistency and maximize therapeutic efficacy, particularly for drug delivery applications. By addressing critical methodological gaps and introducing temporal considerations, this work establishes a framework for scalable, high-quality GELNP production.

植物源性外泌体样纳米颗粒（PELNPs）是一种很有前途的生物活性药物，姜源性外泌体样纳米颗粒（GELNPs）因其抗炎、抗氧化和神经保护特性而表现出显著的治疗潜力。虽然聚乙二醇（PEG）为PELNP分离提供了一种具有成本效益的超离心替代方法，但需要改进方法以提高产量、稳定性和可重复性。本研究在现有生姜纳米囊泡方案的基础上，通过整合PEG6000沉淀、pH调节和0.45 µm过滤，系统地优化了GELNP分离。我们提出了pH调制与过滤的第一个比较评估，展示了不同的权衡：pH调节的GELNPs实现了更小的尺寸，而过滤的GELNPs表现出更好的胶体稳定性和更低的多分散性指数（PDI）。此外，我们确定了先前未报道的GELNP性质的季节性变化，与其他月份分离的纳米颗粒相比，7月份衍生的纳米颗粒表现出更高的稳定性和更小的尺寸。综合的物理化学表征-包括透射电子显微镜（TEM），原子力显微镜（AFM），粒度分析（PSA）， zeta电位分析和傅里叶变换红外光谱(FT-IR) -证实了形态，尺寸分布，表面电荷和生物分子组成的差异。这些发现强调了季节性调整、优化分离方案的必要性，以确保批次间的一致性和最大限度地提高治疗效果，特别是在药物输送应用方面。通过解决关键的方法差距和引入时间考虑，这项工作建立了一个可扩展的、高质量的GELNP生产框架。

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引用次数: 0

Optimization of cellulase-assisted hydrodistillation for Homalomena occulta essential oil: A response surface methodology approach 纤维素酶辅助加氢精馏工艺优化：响应面法

IF 3.8 2区农林科学 Q1 PLANT SCIENCES

Journal of Applied Research on Medicinal and Aromatic Plants

Pub Date : 2025-07-08 DOI: 10.1016/j.jarmap.2025.100647

Ky-Quang Tran Le , Dai Dinh Dang , Thao-Tran Thi Nguyen

Species belonging to the genus Homalomena naturally grow in tropical climates, particularly in areas with high humidity, such as the tropical forests of Vietnam. Essential oils extracted from plants in this genus are currently considered to be expensive. This study focused on the rhizome essential oil of Homalomena occulta (Lour.) Schott., collected in Gia Lai province, and aimed to optimize the extraction process through cellulase-assisted hydrodistillation. Using response surface methodology (RSM) with a central composite design (CCD), the study evaluated the effects of three factors—pH level, enzyme concentration, and incubation time—on the extraction yield. The optimal conditions identified were a cellulase concentration of 0.25 % at a pH of 3.5 for an incubation period of 5 h. Under these conditions, there was a three-fold increase in yield (0.321 ± 0.016 %) compared to the control (0.100 ± 0.011 %). When comparing essential oil samples extracted without cellulase to those extracted using cellulase under optimal conditions, it was evident that the oils obtained from cellulase-treated materials had a higher yield without compromising quality. The organoleptic and physical properties remained largely unchanged; the essential oil retained a transparent, pale yellow color and a sweet, fresh aroma. However, the sample from the cellulase-treated material had a stronger woody scent. The physical properties demonstrated minimal variation, with specific gravities of 0.877 ± 0.0026 g/cm³ and 0.888 ± 0.0016 g/cm³ , respectively. The refractive indices were 1.4671 ± 0.00020 and 1.4680 ± 0.00046, while the optical rotation angles changed insignificantly (-10.388 ± 0.3506 vs. −9.271 ± 0.1851). The essential oil from Homalomena remains rich in natural aromatic compounds, including linalool and various oxygenated compounds such as terpinene-4-ol, α-terpineol, epi-α-muurol, and α-cadionol, all of which have biological value and potential applications.

Homalomena属的物种自然生长在热带气候中，特别是在高湿度的地区，如越南的热带森林。从该属植物中提取的精油目前被认为是昂贵的。本研究主要对隐花蒿（Homalomena occulta）根茎精油进行了研究。Schott。该研究旨在通过纤维素酶辅助加氢蒸馏优化提取工艺。采用响应面法（RSM）和中心复合设计（CCD），研究了ph、酶浓度和孵育时间对提取率的影响。确定的最佳条件是纤维素酶浓度为0.25 %，pH为3.5，孵育时间为5 h。在此条件下，产量（0.321 ± 0.016 %）比对照（0.100 ± 0.011 %）提高了3倍。当比较未使用纤维素酶提取的精油样品与在最佳条件下使用纤维素酶提取的精油样品时，很明显，从纤维素酶处理的材料中获得的油在不影响质量的情况下具有更高的收率。感官和物理性质基本保持不变；精油保留了透明的淡黄色和甜美清新的香气。然而，从纤维素酶处理的材料样品有更强的木香。其物理性质变化最小，比重分别为0.877 ± 0.0026 g/cm³ 和0.888 ± 0.0016 g/cm³ 。折射率分别为1.4671 ± 0.00020和1.4680 ± 0.00046，旋光角变化不大（-10.388 ± 0.3506 vs - 9.271 ± 0.1851）。黄樟精油中含有丰富的天然芳香族化合物，包括芳樟醇和各种含氧化合物，如松油烯-4-醇、α-松油醇、外皮-α-穆罗、α-二醇等，具有一定的生物学价值和潜在的应用价值。

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引用次数: 0

Supercritical fluid extraction of Bidens pilosa L. oil: Kinetics evaluation and antitumor activity 槐油超临界萃取：动力学评价及抗肿瘤活性

IF 3.8 2区农林科学 Q1 PLANT SCIENCES

Journal of Applied Research on Medicinal and Aromatic Plants

Pub Date : 2025-05-01 DOI: 10.1016/j.jarmap.2025.100641

Ramon G. Paschoal , Marianne L. Higino , Ana Paula G. Alves , André Luiz F. Sampaio , Dulcineia F. Teixeira , Leonardo Lucchetti , Marisa F. Mendes

Different plants have been used, around the world, in the treatment of primary diseases as an alternative or popular medicine. In Brazil, the Health Minister introduced a national politic for the phytotherapy use in the Single Health System (SUS). Due to that, Bidenspilosa L. (Asteraceae) was chosen because it is one of the medicinal plants present in the Single Health System list, called RENISUS. Bidens pilosa L. is a popular plant used in the treatment of different diseases (malaria, cancer tumors, fever, diabetes, infections, inflammations, angina, etc.) and is widely disseminated throughout the tropical and subtropical regions of the world. The biological activities of the plant are attributed to the presence of flavonoids, polyacetylenes and terpenes. Therefore, this study evaluated the biological activities of the oil extraction using supercritical CO₂ at different pressure and temperature conditions, due to the scarcity of studies comparing supercritical fluid extraction to conventional techniques (Soxhlet and maceration). The experimental procedure used 8 g of previously dried and milled leaves at different conditions according to an experimental design (150 bar - 60 °C; 179 bar - 46 °C; 179 bar - 74 °C; 250 bar - 40 °C; 250 bar - 80 °C; 321 bar - 46 °C; 321 bar - 74 °C, 350 bar - 60 °C, 250 bar - 60 °C). According to the results, the best yield was 0.082 % (6.6 mg) at 321 bar and 74 °C. The analyses of the experiments done at 321 bar at 74 °C and 250 bar at 40 °C, showed ultraviolet spectra characteristic of terpenes. The extracts analyzed showed promising inhibition in the two tumor cell lines: melanoma, SK-MEL-28 (68 %) and leukemia K562 (90 %), both at a concentration of 150 μg/mL. The results suggested the importance of the bioactive components from Bidens pilosa to contribute to a new anticancer drug using supercritical technology.

在世界各地，不同的植物已被用作治疗原发性疾病的替代药物或流行药物。在巴西，卫生部长提出了在单一卫生系统（SUS）中使用植物疗法的国家政策。因此，选择Bidenspilosa L. （Asteraceae）是因为它是单一卫生系统清单（称为RENISUS）中存在的药用植物之一。Bidens pilosa L.是一种流行的植物，用于治疗不同的疾病（疟疾、癌症肿瘤、发烧、糖尿病、感染、炎症、心绞痛等），广泛分布在世界的热带和亚热带地区。该植物的生物活性归因于黄酮类化合物、聚乙炔和萜烯的存在。因此，由于缺乏将超临界流体萃取与常规技术（索氏和浸渍）进行比较的研究，因此，本研究评估了超临界CO2在不同压力和温度条件下的生物活性。实验程序使用8 g先前干燥和研磨的叶子，根据实验设计(150 bar - 60°C；179 bar - 46°C；179 bar - 74°C；250 bar - 40℃；250 bar - 80℃；321 bar - 46°C；321 bar - 74°C, 350 bar - 60°C, 250 bar - 60°C)。结果表明，在321 bar、74℃条件下，最佳产率为0.082 %（6.6 mg）。在74°C下321 bar和40°C下250 bar进行的实验分析显示了萜烯的紫外光谱特征。所分析的提取物对两种肿瘤细胞系SK-MEL-28（68 %）和白血病K562（90 %）均有抑制作用，浓度均为150 μg/mL。研究结果表明，从毛缕草中提取的活性成分对利用超临界技术开发新型抗癌药物具有重要意义。

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引用次数: 0

Resolving taxonomic complexities in medicinal Aralia: Genome-scale phylogeny and super-barcoding advance species identification and conservation 解决药用植物分类的复杂性：基因组尺度的系统发育和超条形码促进物种鉴定和保护

IF 3.8 2区农林科学 Q1 PLANT SCIENCES

Journal of Applied Research on Medicinal and Aromatic Plants

Pub Date : 2025-05-01 DOI: 10.1016/j.jarmap.2025.100643

Yanan Cao , Menghao Wang , Jiamei Li , Ying Yang , Hao Wang , Yanyan Liu , Hongwei Wang , Risong Na

Aralia section Dimorphanthus represents the largest section in Araliaceae and has been employed in East Asian traditional medicine for centuries. However, the morphological complexity and the prevalence of hybridization/introgression posed a great challenge for accurate species identification, thereby hindering the development, conservation and application of these medicinal plants. In this study, we employed sequences of hundreds of nuclear loci and the complete plastomes to reconstruct the phylogenetic relationships, and evaluated the effectiveness of 12 distinct barcode systems for distinguishing Chinese species of Aralia sect. Dimorphanthus. We found that plastome length, gene content, and order of these species were highly conserved, while the inverted repeat regions showed variability between temperate and tropical/subtropical lineages, providing novel markers for geographical traceability. Phylogenetic relationships constructed based on the genome-scale data confirmed that Chinese Aralia sect. Dimorphanthus was monophyletic and comprised three well-supported lineages. Incomplete lineage sorting and hybridization were identified as the main factors contributing to the phylogenetic conflicts among trees. Species discrimination analysis revealed that super-barcoding strategies integrating complete plastomes and nrDNA sequences, achieved a 92 % species identification accuracy among the 14 studied species of Aralia sect. Dimorphanthus, outperforming standard barcodes. While this system resolved most taxonomic ambiguities, the inclusion of universal nuclear markers is crucial to resolve ambiguities in highly variable taxa like A. elata. These advancements directly support industrial needs for reliable species authentication and biodiversity conservation in pharmacologically important species.

双菊属植物是五加科植物中最大的一种，在东亚的传统医学中已经使用了几个世纪。然而，由于其形态的复杂性和杂交/渗入现象的普遍存在，给准确的物种鉴定带来了很大的挑战，从而阻碍了这些药用植物的开发、保护和应用。本研究利用数百个核位点的序列和完整的质粒进行了系统发育关系的重建，并对12种不同的条形码系统进行了鉴定。研究发现，这些物种的质体体长度、基因含量和序列高度保守，而倒置重复区在温带和热带/亚热带谱系之间表现出差异，为地理溯源提供了新的标记。基于基因组尺度数据构建的系统发育关系证实，中国异花楤木组为单系系，由3个支持良好的谱系组成。谱系分选不全和杂交不全是造成树间系统发育冲突的主要因素。物种鉴别分析表明，采用整合全质体和nrDNA序列的超条形码策略，对菊苣属（Aralia sect. Dimorphanthus） 14种植物的物种鉴定准确率达到92 %，优于标准条形码。虽然该系统解决了大多数分类上的歧义，但通用核标记的包含对于解决像elata这样高度可变的分类群的歧义至关重要。这些进步直接支持工业对可靠的物种认证和生物多样性保护的需求。

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引用次数: 0

Effective SSR markers for assessing population structure, genetic diversity, and volatile compounds in Atractylodis Rhizoma 白术群体结构、遗传多样性和挥发性物质评价的有效SSR标记

IF 3.8 2区农林科学 Q1 PLANT SCIENCES

Journal of Applied Research on Medicinal and Aromatic Plants

Pub Date : 2025-05-01 DOI: 10.1016/j.jarmap.2025.100642

Haotian Zhong , Lina Chen , Lei Chen , Xiao Huang , Ling Gong , Juan Hu , Yuling Zeng , Kun Yu , Lin Sen

In this study, we aimed to develop SSR molecular markers for assessing the population structure and genetic diversity of Atractylodes lancea (AL) and Atractylodes chinensis (AC), with a focus on their volatile compounds. By utilizing transcriptome data from AL, we successfully developed 29 pairs of highly polymorphic SSR primers, generating 264 distinct alleles. Cluster analysis revealed three distinct populations: Mao Mountain, Dabie Mountain, and samples from other locations and primers with high discriminatory power were selected. Additionally, a representative core subset of 106 samples was selected from the original germplasm, capable of representing over 99 % of the genetic variation. This core subset provides an efficient resource for future studies on population genetics and trait association. The Support Vector Machine (SVM)-based method achieved an accuracy of 98.8 % in distinguishing Atractylodis Rhizoma (AR) varieties. GC-MS screening identified 24 potential differential metabolites between the two species, with correlation analysis showing significant association. This study successfully developed SSR molecular markers for analyzing genetic diversity in AL and AC and established a reliable method for identifying AR medicinal varieties. These findings provide a solid foundation for future quality control of AR and the selection of superior germplasm.

本研究旨在利用SSR分子标记对苍术（Atractylodes lancea， AL）和中国苍术（Atractylodes chinensis， AC）的居群结构和遗传多样性进行评价，重点分析其挥发性成分。利用AL的转录组数据，我们成功开发了29对高多态性SSR引物，产生264个不同的等位基因。聚类分析显示，毛山、大别山和其他地区的样本具有明显的种群特征，并选择了判别能力强的引物。此外，从原始种质中选择了106个样本的代表性核心子集，能够代表99% %以上的遗传变异。这一核心亚群为今后的群体遗传学和性状关联研究提供了有效的资源。基于支持向量机（SVM）的方法对苍术品种的鉴别准确率达到98.8 %。GC-MS筛选鉴定出24种潜在的两种差异代谢物，相关分析显示相关性显著。本研究成功开发了用于分析AR和AC遗传多样性的SSR分子标记，为AR药用品种的鉴定建立了可靠的方法。这些研究结果为今后AR的质量控制和优质种质的选择提供了坚实的基础。

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引用次数: 0

Blockchain technology in herbal medicines: Applications, trends, and prospects 区块链技术在草药中的应用、趋势和前景

IF 3.8 2区农林科学 Q1 PLANT SCIENCES

Journal of Applied Research on Medicinal and Aromatic Plants

Pub Date : 2025-05-01 DOI: 10.1016/j.jarmap.2025.100645

Mingyu Han , Qiang Hu , Yuanzhong Wang

The quality and safety of Herbal medicines (HMs) not only affect the development of the industry chain, but also the life safety rights and interests of patients. Traditional HMs supply chains suffer from opaque information and easy tampering, but blockchain intervention can significantly improve the security and traceability of information. The combination of blockchain and herb chain will be the future trend. This paper uses bibliometric statistics to analyze the practical application scenarios of blockchain and summarizes the challenges faced in HMs supply chain. It further explores the application potential of blockchain in the field of HMs and designs a traceability system for HMs based on blockchain technology. A large number of literature surveys found that the application scope of blockchain in HMs is relatively small, and comprehensive coverage of blockchain applications has a high coefficient of difficulty. This paper provides more possibilities for the application of blockchain in HMs, which will promote the development of a benign cycle of the HMs industry chain and protect the rights and interests of consumers.

中药材的质量安全问题不仅关系到产业链的发展，也关系到患者的生命安全权益。传统的医疗器械供应链存在信息不透明和易被篡改的问题，而区块链干预可以显著提高信息的安全性和可追溯性。区块链与草药连锁的结合将是未来的趋势。本文运用文献计量统计学分析了区块链的实际应用场景，总结了医疗保健企业供应链面临的挑战。进一步探索区块链在医疗器械领域的应用潜力，设计了基于区块链技术的医疗器械溯源系统。大量文献调查发现，区块链在HMs中的应用范围相对较小，区块链应用的综合覆盖难度系数较高。本文为区块链在酒店管理行业的应用提供了更多的可能性，促进酒店管理行业产业链良性循环的发展，保护消费者权益。

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引用次数: 0

Xenia effect enhance pod and seed quality in Gleditsia sinensis Xenia效应提高了皂荚和种子的品质

IF 3.8 2区农林科学 Q1 PLANT SCIENCES

Journal of Applied Research on Medicinal and Aromatic Plants

Pub Date : 2025-05-01 DOI: 10.1016/j.jarmap.2025.100644

Qingying Zeng , Yang Zhao , Xiurong Wang , Meixuan Xie , Fuhua Liu , Xingfu Zhang

Gleditsia sinensis is a tree species with high economic value, but the pairing of male and female trees is often overlooked in its cultivation practices. The fruit quality of G. sinensis under natural pollination tends to be inconsistent, resulting in lower economic value. Therefore, understanding the xenia effects of G. sinensis on fruit and seed quality is crucial for improving their overall quality. In this study, The G. sinensis as the female parent and Gleditsia japonica var. delavayi, Gleditsia japonica, G. sinensis, and Gleditsia fera as male parents for hybridization. By observing and comparing the inflorescence characteristics of different male parents, as well as the fruit set rates, pod phenotypes, and seed yield under various pollination combinations, we found that artificial pollination significantly increased the yield of pods and seeds, and distinct xenia effects were observed in the fruits and seeds of G. sinensis. Through comprehensive evaluation, G. fera was identified as the optimal pollen source for interspecific hybridization with G. sinensis, significantly increasing fruit set rate, saponin content, and seed yield. Pollination with G. japonica resulted in a higher seed abortion rate and poorer seed quality, but significantly increased the amino acid and starch content in the pods. G. delavayi pollen is more inclined to produce high-quality seeds. These findings provide valuable insights for the selection of G. sinensis pollinating trees and the improvement of G. sinensis fruit quality.

Data availability

All data generated in this study are presented within the manuscript. Further information and requests could be directed to the lead contact, Prof. Yang Zhao ([email protected])

皂荚是一种具有较高经济价值的树种，但在皂荚的栽培实践中，往往忽略了雌雄树的配对。在自然授粉条件下，白杨果实品质趋于不一致，经济价值较低。因此，了解赤霉素对赤霉素果实和种子品质的影响，对提高赤霉素果实和种子的整体品质具有重要意义。本研究以中国皂荚为雌性亲本，皂荚为雄性亲本，对皂荚变种delavayi、皂荚、中国皂荚和fera皂荚进行杂交。通过观察和比较不同父本的花序特征，以及不同授粉组合下的坐果率、荚果表型和种子产量，我们发现人工授粉显著提高了白杨的荚果和种子产量，并且在白杨的果实和种子中观察到明显的异种效应。通过综合评价，确定fera是与sinensis进行种间杂交的最佳花粉源，可以显著提高坐果率、皂苷含量和种子产量。用粳稻授粉后，籽粒败育率较高，籽粒质量较差，但荚果中氨基酸和淀粉含量显著增加。花粉更倾向于产生优质种子。这些研究结果为中华猕猴桃授粉树种的选择和中华猕猴桃果实品质的改良提供了有价值的见解。数据可得性本研究中产生的所有数据均在论文中列出。进一步的信息和请求可以直接联系首席联系人赵杨教授（[email protected]）。

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引用次数: 0

Characterizing trait-specific populations and genetic diversity patterns in clary sage (Salvia sclarea L.) using SSR markers 利用SSR标记分析鼠尾草（Salvia sclarea L.）的性状特异性群体和遗传多样性模式

IF 3.8 2区农林科学 Q1 PLANT SCIENCES

Journal of Applied Research on Medicinal and Aromatic Plants

Pub Date : 2025-05-01 DOI: 10.1016/j.jarmap.2025.100646

Niketa Yadav , Mohar Singh , Asmita Saini , Pratibha Pandey , Garima Thakur , Ramesh Chauhan , Satbeer Singh , Sanatsujat Singh

Clary sage (Salvia sclarea L.) is an aromatic and medicinal herb grown widely across Europe, Western Asia, Maghreb, the Mideast, and countries like India. The purpose of this study was to assess genetic diversity of clary sage genotypes from the Western Himalayas, aiming to support future breeding efforts. A total of 72 genotypes of clary sage from six distinct populations were examined using 49 simple sequence repeat markers. In total, 323 alleles were detected ranging from 1 to 16 with an average of 6.54 alleles per locus. Among these, 78.33 % were common alleles, 1.86 % were rare, and 19.81 % were frequent alleles. The results revealed an overall polymorphic information content of 93.88 %, with an average of 0.71 ranging from 0.00 to 0.93 at each marker locus, indicating high genetic variability among the studied material. The detected heterozygosity (0.52) was lower than expected heterozygosity (0.71), pointing a heterozygotes deficit populations. Analysis of variance showed considerable variability for biomass yield. Molecular analysis of variance (AMOVA) showed that genetic diversity was greater within individuals than among populations. Unweighted neighbour-joining, principal coordinate analysis, and population structure showed that the genotypes from different geographic regions and various biomass yield groups were predominantly admixed across clusters. The cluster-III represented the Bharmour population was the highest biomass performer group. The observed diversity patterns will facilitate plant breeders to make choices for breeding programs aiming for higher biomass yield in this plant.

鼠尾草（Salvia sclarea L.）是一种芳香和药用草本植物，广泛生长在欧洲、西亚、马格里布、中东和印度等国家。本研究的目的是评估西喜马拉雅地区鼠尾草基因型的遗传多样性，旨在为今后的育种工作提供支持。利用49个简单序列重复标记对6个不同居群的鼠尾草共72个基因型进行了分析。共检测到1 ~ 16个等位基因323个，平均每个位点检测到6.54个等位基因。其中78.33 %为常见等位基因，1.86 %为罕见等位基因，19.81 %为常见等位基因。结果表明，总体多态性信息含量为93.88 %，每个标记位点的平均多态性信息含量为0.71，范围在0.00 ~ 0.93之间，表明材料间具有较高的遗传变异性。检测到的杂合度（0.52）低于预期的杂合度（0.71），表明存在杂合度不足的群体。方差分析表明，生物量产量具有相当大的变异性。分子变异分析（AMOVA）表明，遗传多样性在个体内大于群体间。非加权邻联分析、主坐标分析和种群结构分析表明，不同地理区域和不同生物量产量群体的基因型在集群间以混合为主。聚类iii代表巴摩种群是生物量表现最高的群体。观察到的多样性模式将有助于植物育种者选择以提高该植物生物量产量为目标的育种计划。

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引用次数: 0

Assessment of the quality of plant material in commercial products of ground ivy herb (Glechomae hederaceae herba) 藤属植物商品原料质量评价（英文）

IF 3.8 2区农林科学 Q1 PLANT SCIENCES

Journal of Applied Research on Medicinal and Aromatic Plants

Pub Date : 2025-04-01 DOI: 10.1016/j.jarmap.2025.100634

Karolina Grabowska, Weronika Ziółkowska-Żygas, Agnieszka Galanty, Dagmara Wróbel-Biedrawa, Irma Podolak

G. hederacea has a well-established position in traditional medicinal systems of Europe and Asia. Despite the significant increase in the number of products based on this plant on the European market, there is as yet no European Pharmacopoeia monograph on Glechomae herba, therefore legally binding quality standards of medical-grade ground ivy herb in member states that ratified Convention on the elaboration of a European Pharmacopoeia. This study aimed to investigate differences in the quality of the commercially available single-herb loose G. hederacea products in the aspects of authentication, purity of plant material and content of bioactive substances. As the therapeutic potential of G. hederacea is related to the presence of phenolic compounds, the study on qualitative and quantitative analysis of phenolics by HPLC method was performed. The analysis revealed significant differences in the quality of ground ivy plant material, in terms of the content of bioactive compounds. The determined levels of rosmarinic acid, chlorogenic acid, gallic acid and rutin in the products ranged from 0.10 to 4.55 mg/g DW; 0.18–1.57 mg/g DW; 0.02–1.28 mg/g DW; to 6.01 mg/g DW, respectively. Additionally, the products varied significantly in the sum of determined polyphenols (1.5 – 7.16 mg/g DW). The findings highlight the need to establish the standards and requirements for bioactive phenolic content to ensure high-quality plant material with significant medicinal value. Additionally, our study suggests that rosmarinic acid, rutin, chlorogenic acid, and the total polyphenol content could serve as quality markers for Glechomae hederaceae herba in pro-health applications.

在欧洲和亚洲的传统医药体系中，白头翁有着良好的地位。尽管欧洲市场上以这种植物为基础的产品数量显著增加，但迄今为止还没有关于Glechomae herba的欧洲药典专著，因此，在批准《制定欧洲药典公约》的成员国中，具有法律约束力的医疗级地面常春藤草药质量标准。本研究旨在探讨市售单一药材麻花散装产品在鉴定、原料纯度和生物活性物质含量等方面的质量差异。由于麻属植物的治疗潜力与酚类化合物的存在有关，因此采用高效液相色谱法对麻属植物中的酚类物质进行了定性和定量分析。分析表明，在生物活性化合物含量方面，地面常春藤植物材料的质量存在显著差异。产品中迷迭香酸、绿原酸、没食子酸和芦丁的含量为0.10 ~ 4.55 mg/g DW；0.18 - -1.57 毫克/克DW,0.02 - -1.28 毫克/克DW,分别为6.01 mg/g DW。此外，两种产品的多酚含量差异显著（1.5 - 7.16 mg/g DW）。研究结果表明，有必要建立生物活性酚含量的标准和要求，以确保具有重要药用价值的高质量植物材料。此外，本研究提示迷迭香酸、芦丁、绿原酸和总多酚含量可作为中药的质量评价指标。

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引用次数: 0

Identification of Dendrobium officinale species and authentication of commercial products by DNA barcoding and high-resolution melting analysis 利用DNA条形码和高分辨率熔融分析鉴定铁皮石斛的种类及商品鉴定

IF 3.8 2区农林科学 Q1 PLANT SCIENCES

Journal of Applied Research on Medicinal and Aromatic Plants

Pub Date : 2025-04-01 DOI: 10.1016/j.jarmap.2025.100633

Zhilong He , Zhao Yang , Cui Dong , Shanshan Yan , Jing Lin , Hexigeduleng Bao , Fan Tang , Leilei Zhang , Cheng Zhu , Yan Hu , Yanfei Ding

Dendrobium officinale is widely used as a precious traditional medicinal plant and health food in China. However, because of its high nutritional and medicinal value, it is often targeted for adulteration or mislabeling. This work aims to exploit DNA barcoding combined with high-resolution melting analysis to differentiate Dendrobium officinale（D. officinale）species from non-D. officinale species and to further identify its botanical origin. The internal transcribed spacer 2 (ITS2) was targeted as a candidate barcode for Dendrobium species identification. High resolution melting（HRM）method allowed differentiating the six Dendrobium species in three clusters with 95 % confidence interval, which is consistent with the sequencing analysis. ITS2 sequencing results implied that 43 % of commercial Dendrobium products may be mislabeled. Thus, ITS2 DNA barcoding with HRM assay could be used as a simple, specific and cost-effective tool to identify different Dendrobium species and commercial Dendrobium products, and to authenticate the origin of D. officinale.

铁皮石斛是一种珍贵的传统药用植物和保健食品，在中国被广泛使用。然而，由于其高营养和药用价值，它经常成为掺假或贴错标签的目标。本工作旨在利用DNA条形码结合高分辨率熔融分析来区分铁皮石斛（ddrobium officinale）。officinale)来自非d。Officinale的种类，并进一步确定其植物来源。内部转录间隔器2 （ITS2）被定位为石斛物种鉴定的候选条形码。高分辨率熔融（HRM）方法可将6种石斛分为3个簇，置信区间为95 %，与测序分析结果一致。ITS2测序结果表明43 %的石斛商业产品可能存在标签错误。因此，ITS2 DNA条形码与HRM技术可作为一种简单、特异、经济的工具，用于不同石斛品种和商品石斛产品的鉴定，并对铁皮石斛的来源进行鉴定。

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引用次数: 0