Pub Date : 2024-04-15DOI: 10.9734/jocamr/2024/v25i4529
Okpukpara O. Bernardine, Achukwu U. Peter, Kafor N. Bernard
Aims: To investigate the effect of pooled extract of Annona muricata (A. muricata) and Allium sativum (A. sativum) on testosterone-induced prostate hyperplasia in male albino rats. Experimental Procedure: Male albino rats for the study were divided into seven groups identified as A-G groups. Group A and G were used as negative and positive controls with 12 and 5 rats respectively. Male rats in group B-G were induced with 3mg/kg body weight of testosterone propionate injection subcutaneously for 28 days. Animals in group B and C received 100mg/kg body weight of individual Annona muricata and Allium sativum extract respectively, while animals in group D-F received 100mg/kg, 300mg/kg and 600mg/kg body weight of the pooled extracts. We evaluated relative prostate weight(PI),serum prostate specific antigen(PSA),interleukins-6(IL-6), total protein, and histological changes including KI 67 and BCL2 expressions. Results and Conclusion: The study revealed that pooled extracts of A. muricata and A. sativum significantly inhibited prostatic hyperplasia; decreased PI,serum PSA,IL-6, total protein, and expression of KI 67, far better than the any of the single plant extract in this study. This suggests that pooled extract of A, muricata and A, sativum may be used as a therapeutic agent for prostatic hyperplasia, as a result of the influence of the phytochemical constituents.
目的：研究鼠尾草和薤白的提取物对睾酮诱导的雄性白化大鼠前列腺增生的影响。实验过程：用于研究的雄性白化大鼠分为七组，分别为 A-G 组。A 组和 G 组分别有 12 只和 5 只大鼠作为阴性和阳性对照组。B-G 组雄性大鼠皮下注射 3 毫克/千克体重的丙酸睾酮，连续 28 天。B 组和 C 组大鼠分别接受 100 毫克/千克体重的单个鼠李提取物和薤白提取物，D-F 组大鼠分别接受 100 毫克/千克、300 毫克/千克和 600 毫克/千克体重的混合提取物。我们对相对前列腺重量（PI）、血清前列腺特异性抗原（PSA）、白细胞介素-6（IL-6）、总蛋白以及组织学变化（包括 KI 67 和 BCL2 的表达）进行了评估。结果与结论：研究表明，A. muricata 和 A. sativum 的混合提取物对前列腺增生有明显的抑制作用，能降低 PI、血清 PSA、IL-6、总蛋白和 KI 67 的表达，远优于本研究中的任何单一植物提取物。这表明，由于植物化学成分的影响，A, muricata 和 A, sativum 的混合提取物可用作前列腺增生的治疗剂。
{"title":"Histopathological and Biochemical Assessment of Annona muricata and Allium sativum Pooled Extract on Testosterone Induced Prostate Hyperplasia in Albino Rats","authors":"Okpukpara O. Bernardine, Achukwu U. Peter, Kafor N. Bernard","doi":"10.9734/jocamr/2024/v25i4529","DOIUrl":"https://doi.org/10.9734/jocamr/2024/v25i4529","url":null,"abstract":"Aims: To investigate the effect of pooled extract of Annona muricata (A. muricata) and Allium sativum (A. sativum) on testosterone-induced prostate hyperplasia in male albino rats. \u0000Experimental Procedure: Male albino rats for the study were divided into seven groups identified as A-G groups. Group A and G were used as negative and positive controls with 12 and 5 rats respectively. Male rats in group B-G were induced with 3mg/kg body weight of testosterone propionate injection subcutaneously for 28 days. Animals in group B and C received 100mg/kg body weight of individual Annona muricata and Allium sativum extract respectively, while animals in group D-F received 100mg/kg, 300mg/kg and 600mg/kg body weight of the pooled extracts. We evaluated relative prostate weight(PI),serum prostate specific antigen(PSA),interleukins-6(IL-6), total protein, and histological changes including KI 67 and BCL2 expressions. \u0000Results and Conclusion: The study revealed that pooled extracts of A. muricata and A. sativum significantly inhibited prostatic hyperplasia; decreased PI,serum PSA,IL-6, total protein, and expression of KI 67, far better than the any of the single plant extract in this study. This suggests that pooled extract of A, muricata and A, sativum may be used as a therapeutic agent for prostatic hyperplasia, as a result of the influence of the phytochemical constituents.","PeriodicalId":15398,"journal":{"name":"Journal of Complementary and Alternative Medical Research","volume":"303 9","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140703440","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-04-10DOI: 10.9734/jocamr/2024/v25i4528
Mohammed Abdul-Aziz Ali, N. Dhahir
Background: Thalassemia is a widespread heritable disease in Mediterranean region, the assessment of psychological and social status of thalassemic children is an important issue. Objectives: The main aim is to assess psychosocial status of children with thalassemia and to find out the association between some demographic characteristics and psychosocial aspects. Methodology: A cross-sectional study of (101) randomly selected thalassemic children. The study was conducted at Al-Hadbaa Specialist Hospital for blood diseases and bone marrow transplantation, Mosul City, Iraq for the period from 1st November 2023 to 5th January 2024.In order to collect the required data, a questionnaire was developed based on the standards of the pediatric quality of life Scale (PedsQoL). Results: shows that the largest proportion of samples belong to the age group (9-12) years and the highest percentage of the study sample is female (50.5%).Regarding education, the table presents that the highest percentage of patients are student (67.3%). The table also shows that (65.3%) of patients have family history of thalassemia. For the number of blood transfusion, the highest percentage was (every 3 weeks) to be (59.4%). The mean+SD emotional domain score was 1.8733±0.602, social domain score was 1.2436±3.55. Conclusion: The present study indicates that psychosocial aspects were range between moderate for emotional functioning and good for social functioning. And there is significant association between some demographic variables and psychosocial functioning of thalassemic children. Recommendations: The current study recommended to provide psychological and social support units for thalassemic children to help them to adapt with the disease by reducing negative thoughts about themselves and building a positive self-image.
{"title":"Psychosocial Aspects of Children with Thalassemia in Mosul City","authors":"Mohammed Abdul-Aziz Ali, N. Dhahir","doi":"10.9734/jocamr/2024/v25i4528","DOIUrl":"https://doi.org/10.9734/jocamr/2024/v25i4528","url":null,"abstract":"Background: Thalassemia is a widespread heritable disease in Mediterranean region, the assessment of psychological and social status of thalassemic children is an important issue. \u0000Objectives: The main aim is to assess psychosocial status of children with thalassemia and to find out the association between some demographic characteristics and psychosocial aspects. \u0000Methodology: A cross-sectional study of (101) randomly selected thalassemic children. The study was conducted at Al-Hadbaa Specialist Hospital for blood diseases and bone marrow transplantation, Mosul City, Iraq for the period from 1st November 2023 to 5th January 2024.In order to collect the required data, a questionnaire was developed based on the standards of the pediatric quality of life Scale (PedsQoL). \u0000Results: shows that the largest proportion of samples belong to the age group (9-12) years and the highest percentage of the study sample is female (50.5%).Regarding education, the table presents that the highest percentage of patients are student (67.3%). The table also shows that (65.3%) of patients have family history of thalassemia. For the number of blood transfusion, the highest percentage was (every 3 weeks) to be (59.4%). The mean+SD emotional domain score was 1.8733±0.602, social domain score was 1.2436±3.55. \u0000Conclusion: The present study indicates that psychosocial aspects were range between moderate for emotional functioning and good for social functioning. And there is significant association between some demographic variables and psychosocial functioning of thalassemic children. \u0000Recommendations: The current study recommended to provide psychological and social support units for thalassemic children to help them to adapt with the disease by reducing negative thoughts about themselves and building a positive self-image.","PeriodicalId":15398,"journal":{"name":"Journal of Complementary and Alternative Medical Research","volume":"29 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-04-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140717455","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-04-10DOI: 10.9734/jocamr/2024/v25i4527
Oluwadunni Joan Okwulu, Ibrahim Lawal, Naomi Oghenefega Danjuma-Audu, Mathew Olawale Abatan
Aim: This study aimed to evaluate the ameliorative potentials of S. alata in combination with Selenium on liver and kidney injury induced by Isoniazid-Rifampicin (INH-RIF). Study Design: Original Research work. Place and Duration of Study: Department of Pharmacology and Toxicology, National Institute for Pharmaceutical Research and Development (NIPRD), Idu, Abuja, between August and October 2023. Methods: Hydroethanolic extract of S. alata (EESA) was obtained by maceration, 25 Swiss mice (25-35g) were divided into 5 groups, GP1 received distill water, GP 2-5 INH-RIF 150mg/kg for the first two days; while groups 3-5 received S.alata 100mg/kg, 200mg/kg and 400mg/kg in combinAtion with Selenium 1mg/kg respectively from day 3 till day 14 orally. Sera samples were obtained and organs (liver and kidney) for weighing and histopathology on day 15. Results: This study showed that S.alata (EESA) (100mg/kg) and Selenium (1mg/kg) showed no reduction (P>.05) in the biochemical parameters (AST, ALP, ALT, BUN, Uric acid, and creatinine); histo-anatomy of both organs showed injury. The 200mg/kg EESA/Se (1mg/kg) reduced significantly (P<.05) elevated parameters for liver only (ALT, ALP); liver histo-picture showed no lesion; 400mg/kg combination decreased all parameters significantly (P<.05); histo-pathology showed no lesion in both organs. Weights of the liver showed significant reduction (P=.0025, .0052, and .0027) at 100, 200, and 400mg/kg respectively; but no significant difference in weights of the kidney compared to the toxic group. Conclusion: It was concluded that EESA 200mg/kg with Selenium 1mg/kg can mitigate the toxic effects of Isoniazid-Rifampicin on the liver, while EESA/Se 400mg/kg combination has ameliorative potentials on liver and kidney toxicity from INH-RIF. These effects may be dose-dependent. It was recommended that 400mg/kg of EESA and 1mg/kg of Selenium combination be considered in drug development.
{"title":"Senna alata with Selenium Mitigates Liver and Kidney Injury from Isoniazid and Rifampicin in Mice","authors":"Oluwadunni Joan Okwulu, Ibrahim Lawal, Naomi Oghenefega Danjuma-Audu, Mathew Olawale Abatan","doi":"10.9734/jocamr/2024/v25i4527","DOIUrl":"https://doi.org/10.9734/jocamr/2024/v25i4527","url":null,"abstract":"Aim: This study aimed to evaluate the ameliorative potentials of S. alata in combination with Selenium on liver and kidney injury induced by Isoniazid-Rifampicin (INH-RIF).\u0000Study Design: Original Research work.\u0000Place and Duration of Study: Department of Pharmacology and Toxicology, National Institute for Pharmaceutical Research and Development (NIPRD), Idu, Abuja, between August and October 2023.\u0000 Methods: Hydroethanolic extract of S. alata (EESA) was obtained by maceration, 25 Swiss mice (25-35g) were divided into 5 groups, GP1 received distill water, GP 2-5 INH-RIF 150mg/kg for the first two days; while groups 3-5 received S.alata 100mg/kg, 200mg/kg and 400mg/kg in combinAtion with Selenium 1mg/kg respectively from day 3 till day 14 orally. Sera samples were obtained and organs (liver and kidney) for weighing and histopathology on day 15.\u0000Results: This study showed that S.alata (EESA) (100mg/kg) and Selenium (1mg/kg) showed no reduction (P>.05) in the biochemical parameters (AST, ALP, ALT, BUN, Uric acid, and creatinine); histo-anatomy of both organs showed injury. The 200mg/kg EESA/Se (1mg/kg) reduced significantly (P<.05) elevated parameters for liver only (ALT, ALP); liver histo-picture showed no lesion; 400mg/kg combination decreased all parameters significantly (P<.05); histo-pathology showed no lesion in both organs. Weights of the liver showed significant reduction (P=.0025, .0052, and .0027) at 100, 200, and 400mg/kg respectively; but no significant difference in weights of the kidney compared to the toxic group.\u0000Conclusion: It was concluded that EESA 200mg/kg with Selenium 1mg/kg can mitigate the toxic effects of Isoniazid-Rifampicin on the liver, while EESA/Se 400mg/kg combination has ameliorative potentials on liver and kidney toxicity from INH-RIF. These effects may be dose-dependent. It was recommended that 400mg/kg of EESA and 1mg/kg of Selenium combination be considered in drug development.","PeriodicalId":15398,"journal":{"name":"Journal of Complementary and Alternative Medical Research","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-04-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140717899","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-04-02DOI: 10.9734/jocamr/2024/v25i3525
Ritika Bhardwaj, Richa Kashyap, Puja Sharma
Objective: This comprehensive review delves into the multifaceted impact of Theraband training with blood flow restriction (BFR) on vascular adaptations in women. With a focus on synthesizing current knowledge, the objective is to elucidate the physiological mechanisms, explore clinical applications, and outline potential benefits of this innovative exercise paradigm within the female demographic. Methods: Employing a systematic approach, a thorough review of pertinent literature was conducted using databases such as PubMed, MEDLINE, and Cochrane Library. Inclusion criteria centered on studies investigating the effects of Theraband training with BFR on vascular outcomes in women. Extracted data encompassed study design, participant demographics, intervention specifics, and key findings. Results: The review reveals intricate physiological mechanisms underlying vascular adaptations induced by Theraband training with BFR in women, emphasizing notable changes in blood flow dynamics, nitric oxide production, and endothelial function. Clinical applications are discussed, showcasing the potential of this approach in rehabilitation settings and its efficacy in managing cardiovascular and metabolic conditions unique to the female population. Conclusion: Synthesizing existing evidence, this review underscores the promising role of Theraband training with blood flow restriction in enhancing vascular health in women. By providing comprehensive insights into the physiological intricacies, clinical applications, and potential benefits, this review serves as a pivotal resource for researchers, clinicians, and fitness professionals. It offers guidance for future investigations and practical applications of this innovative exercise strategy, aiming to optimize cardiovascular outcomes specifically for women.
{"title":"Enhancing Vascular Adaptations: A Comprehensive Review on the Impact of Theraband Training with Blood Flow Restriction in Women","authors":"Ritika Bhardwaj, Richa Kashyap, Puja Sharma","doi":"10.9734/jocamr/2024/v25i3525","DOIUrl":"https://doi.org/10.9734/jocamr/2024/v25i3525","url":null,"abstract":"Objective: This comprehensive review delves into the multifaceted impact of Theraband training with blood flow restriction (BFR) on vascular adaptations in women. With a focus on synthesizing current knowledge, the objective is to elucidate the physiological mechanisms, explore clinical applications, and outline potential benefits of this innovative exercise paradigm within the female demographic.\u0000Methods: Employing a systematic approach, a thorough review of pertinent literature was conducted using databases such as PubMed, MEDLINE, and Cochrane Library. Inclusion criteria centered on studies investigating the effects of Theraband training with BFR on vascular outcomes in women. Extracted data encompassed study design, participant demographics, intervention specifics, and key findings.\u0000Results: The review reveals intricate physiological mechanisms underlying vascular adaptations induced by Theraband training with BFR in women, emphasizing notable changes in blood flow dynamics, nitric oxide production, and endothelial function. Clinical applications are discussed, showcasing the potential of this approach in rehabilitation settings and its efficacy in managing cardiovascular and metabolic conditions unique to the female population.\u0000Conclusion: Synthesizing existing evidence, this review underscores the promising role of Theraband training with blood flow restriction in enhancing vascular health in women. By providing comprehensive insights into the physiological intricacies, clinical applications, and potential benefits, this review serves as a pivotal resource for researchers, clinicians, and fitness professionals. It offers guidance for future investigations and practical applications of this innovative exercise strategy, aiming to optimize cardiovascular outcomes specifically for women.","PeriodicalId":15398,"journal":{"name":"Journal of Complementary and Alternative Medical Research","volume":"25 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140753933","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-04-02DOI: 10.9734/jocamr/2024/v25i3526
Rodolphe Koularambaye, Sara Nathalie Edjenguèlè Béboy, Yannick Sani Jignoua, A. F. Feudjio, A. Choupo, Fabiola Jenny Massah, C. A. Pieme, P. Moundipa
Aims: Pycnanthus angolensis (Welw.) Warb. also known as “Etengué” in the Baka language, is a medicinal plant used by the Baka Pygmies of Cameroon to treat erectile dysfunction. This study aimed to evaluate the androgenic activity and subchronic toxicity of the aqueous extract of Pycnanthus angolensis wood. Study Design: Experimental design. Place and Duration of Study: Laboratory of Biochemistry, Faculty of Medicine and Biomedical Sciences, University of Yaoundé I, between November 2022 and October 2023. Methodology: Twenty-five male Wistar strain rats were divided into 5 groups of 5 rats each including a positive control group that received testosterone enanthate (5 mg/kg) intramuscularly once a week; a normal control group that received distilled water (10 ml/kg) and three test groups which received 134 and 267 and 533 mg/kg of the aqueous extract of Pycnanthus angolensis daily per os. On the 29th day, the animals were killed, and several biochemical parameters were assessed for androgenic properties. Results: At a dose of 134 mg/kg, the extract significantly increased (p<0.05) the levels of testicular cholesterol, testosterone and serum prostatic acid phosphatase activity compared to those of the normal control. A toxicity study showed that at doses of 134 and 267 mg/kg, the extract did not induce any significant variation (p>0.05) in the creatinine level, but a significant reduction (p<0.05) in of aspartate and alanine aminotransferase activities was recorded. Histopathology revealed destruction of testicular spermatozoa at a dose of 533 mg/kg compared to that of the controls. Conclusion: These results showed that at a dose of 134 mg/kg, the aqueous extract of Pycnanthus angolensis (Welw.) Warb. wood is capable of stimulating the production of androgens and is tolerated by the rat's body. Our results justified the traditional use of this plant for the treatment of male infertility and erectile dysfunction.
{"title":"Androgenic Properties and Subchronic Toxicity of the Aqueous Extract of Pycnanthus angolensis (Welw.) Warb. Wood (Myristicaceae)","authors":"Rodolphe Koularambaye, Sara Nathalie Edjenguèlè Béboy, Yannick Sani Jignoua, A. F. Feudjio, A. Choupo, Fabiola Jenny Massah, C. A. Pieme, P. Moundipa","doi":"10.9734/jocamr/2024/v25i3526","DOIUrl":"https://doi.org/10.9734/jocamr/2024/v25i3526","url":null,"abstract":"Aims: Pycnanthus angolensis (Welw.) Warb. also known as “Etengué” in the Baka language, is a medicinal plant used by the Baka Pygmies of Cameroon to treat erectile dysfunction. This study aimed to evaluate the androgenic activity and subchronic toxicity of the aqueous extract of Pycnanthus angolensis wood. \u0000Study Design: Experimental design. \u0000Place and Duration of Study: Laboratory of Biochemistry, Faculty of Medicine and Biomedical Sciences, University of Yaoundé I, between November 2022 and October 2023. \u0000Methodology: Twenty-five male Wistar strain rats were divided into 5 groups of 5 rats each including a positive control group that received testosterone enanthate (5 mg/kg) intramuscularly once a week; a normal control group that received distilled water (10 ml/kg) and three test groups which received 134 and 267 and 533 mg/kg of the aqueous extract of Pycnanthus angolensis daily per os. On the 29th day, the animals were killed, and several biochemical parameters were assessed for androgenic properties. \u0000Results: At a dose of 134 mg/kg, the extract significantly increased (p<0.05) the levels of testicular cholesterol, testosterone and serum prostatic acid phosphatase activity compared to those of the normal control. A toxicity study showed that at doses of 134 and 267 mg/kg, the extract did not induce any significant variation (p>0.05) in the creatinine level, but a significant reduction (p<0.05) in of aspartate and alanine aminotransferase activities was recorded. Histopathology revealed destruction of testicular spermatozoa at a dose of 533 mg/kg compared to that of the controls. \u0000Conclusion: These results showed that at a dose of 134 mg/kg, the aqueous extract of Pycnanthus angolensis (Welw.) Warb. wood is capable of stimulating the production of androgens and is tolerated by the rat's body. Our results justified the traditional use of this plant for the treatment of male infertility and erectile dysfunction.","PeriodicalId":15398,"journal":{"name":"Journal of Complementary and Alternative Medical Research","volume":"17 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140753406","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-03-23DOI: 10.9734/jocamr/2024/v25i3522
Nabhan AL-Dhamary, A. Al-Adhal, H. Kadi, M. Al-Kamarany
Background: Aloe is plant possessing a great therapeutic potential in folk medicine in Yemen namely Aloe vera used as a multipurpose skin treatment. Objective: Therefore, this study aimed to investigate the effect of topical Aloe vera gel in fresh form on the wound healing of rabbit model in comparison with Moist Exposed Wound Ointment (MEBO) . Methods: The experimental animals as models of excision wounds that were classified into four groups. The 1st group was not treated as a control, the 2nd group was treated with a placebo (petroleum gel), the 3rd group was treated with Mebo as drug standard, and the 4th group was treated with Aloe vera gel in fresh form. This study was carried out in Sana’a city during the period of three weeks. The healing parameters were epithelization period (scar fall day) and percentage of wound contraction (%) and the data obtained were analyzed. Results: The results showed that the significant difference between study group (p < 0.05) and the effect of this plant on an increase in the percentage (%) of wound contraction was better than the standard drug (Mebo) group and other groups. On the other mean, on the 16th day, the percentage of wound contraction of the treated group with Aloe vera was 100 % while in the treated group with Mebo was 97.6%. In addition, in placebo and control groups were 76.6 % and 73.8 %, respectively. Conclusion: Finally, from these findings, it could be concluded that the application of Aloe vera gel in topically administered to an open wound induces significant wound contraction and accelerates wound healing and this herbal may be a promising medication for open wounds.
{"title":"Investigation Effect of Aloe Vera in Fresh Gel Form on Rabbit’s Model Wound","authors":"Nabhan AL-Dhamary, A. Al-Adhal, H. Kadi, M. Al-Kamarany","doi":"10.9734/jocamr/2024/v25i3522","DOIUrl":"https://doi.org/10.9734/jocamr/2024/v25i3522","url":null,"abstract":"Background: Aloe is plant possessing a great therapeutic potential in folk medicine in Yemen namely Aloe vera used as a multipurpose skin treatment. \u0000Objective: Therefore, this study aimed to investigate the effect of topical Aloe vera gel in fresh form on the wound healing of rabbit model in comparison with Moist Exposed Wound Ointment (MEBO) . \u0000Methods: The experimental animals as models of excision wounds that were classified into four groups. The 1st group was not treated as a control, the 2nd group was treated with a placebo (petroleum gel), the 3rd group was treated with Mebo as drug standard, and the 4th group was treated with Aloe vera gel in fresh form. This study was carried out in Sana’a city during the period of three weeks. The healing parameters were epithelization period (scar fall day) and percentage of wound contraction (%) and the data obtained were analyzed. \u0000Results: The results showed that the significant difference between study group (p < 0.05) and the effect of this plant on an increase in the percentage (%) of wound contraction was better than the standard drug (Mebo) group and other groups. On the other mean, on the 16th day, the percentage of wound contraction of the treated group with Aloe vera was 100 % while in the treated group with Mebo was 97.6%. In addition, in placebo and control groups were 76.6 % and 73.8 %, respectively. \u0000Conclusion: Finally, from these findings, it could be concluded that the application of Aloe vera gel in topically administered to an open wound induces significant wound contraction and accelerates wound healing and this herbal may be a promising medication for open wounds. ","PeriodicalId":15398,"journal":{"name":"Journal of Complementary and Alternative Medical Research","volume":" 19","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140210379","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-03-16DOI: 10.9734/jocamr/2024/v25i3521
Sandra Peter Magnus, M. Anagboso, I. Johnny, U. P. Ise, J. Okokon
Telfairia occidentalis Hook (cucurbitaceae) leaves and seeds, which are used as vegetable in the preparation of soups and as medicine traditionally to treat various diseases by the Ibibios were investigated for genotoxic and cytotoxic effects using Allium cepa test. The effects of the leaves and seeds extracts on the root meristem cells of Allium cepa were investigated using onion bulbs exposed to 2.5 mg/mL, 5 mg/mL, and 10 mg/mL concentrations of the extracts for macroscopic and microscopic analysis. Tap water was used as a negative control and Methotrexate (0.1 mg/ml) was used as a positive control. There was statistically significant (p< 0.05) inhibition of root growth depending on concentration by the extracts when compared with the negative control group. All the tested extracts were observed to have cytotoxic effects on cell division in A. cepa. The extracts-induced chromosomal aberrations and micronuclei (MNC) formations in A. cepa root tip cells were significant (p<0.05) when compared with control group. The extracts treatment further induced cell death, ghost cells, cells membrane damage, and binucleated cells. The seed extract was found to have a higher cytotoxic and genotoxic effects than the leaf extract. These results suggest that the leaf and seeds extract of Telfairia occidentalis possess cytotoxic and genotoxic effects on A. cepa.
Telfairia occidentalis Hook（葫芦科）的叶子和种子被伊比比亚人用作制作汤的蔬菜和治疗各种疾病的传统药物。使用暴露在 2.5 毫克/毫升、5 毫克/毫升和 10 毫克/毫升浓度提取物中的洋葱球茎进行宏观和微观分析，研究了叶子和种子提取物对辣椒根分生组织细胞的影响。自来水用作阴性对照，甲氨蝶呤（0.1 毫克/毫升）用作阳性对照。与阴性对照组相比，不同浓度的提取物对根生长的抑制作用具有统计学意义（p< 0.05）。所有测试提取物都对牛肝菌的细胞分裂有细胞毒性作用。与对照组相比，萃取物诱导的辣椒根尖细胞染色体畸变和微核（MNC）形成显著（p<0.05）。提取物处理进一步诱导细胞死亡、鬼细胞、细胞膜损伤和双核细胞。与叶提取物相比，种子提取物具有更高的细胞毒性和基因毒性作用。这些结果表明，西洋接骨木的叶片和种子提取物对牛肝菌具有细胞毒性和基因毒性作用。
{"title":"Evaluation of Genotoxic and Cytotoxic Activities of Leaf and Seed Extracts of Telfairia occidentalis","authors":"Sandra Peter Magnus, M. Anagboso, I. Johnny, U. P. Ise, J. Okokon","doi":"10.9734/jocamr/2024/v25i3521","DOIUrl":"https://doi.org/10.9734/jocamr/2024/v25i3521","url":null,"abstract":"Telfairia occidentalis Hook (cucurbitaceae) leaves and seeds, which are used as vegetable in the preparation of soups and as medicine traditionally to treat various diseases by the Ibibios were investigated for genotoxic and cytotoxic effects using Allium cepa test. The effects of the leaves and seeds extracts on the root meristem cells of Allium cepa were investigated using onion bulbs exposed to 2.5 mg/mL, 5 mg/mL, and 10 mg/mL concentrations of the extracts for macroscopic and microscopic analysis. Tap water was used as a negative control and Methotrexate (0.1 mg/ml) was used as a positive control. There was statistically significant (p< 0.05) inhibition of root growth depending on concentration by the extracts when compared with the negative control group. All the tested extracts were observed to have cytotoxic effects on cell division in A. cepa. The extracts-induced chromosomal aberrations and micronuclei (MNC) formations in A. cepa root tip cells were significant (p<0.05) when compared with control group. The extracts treatment further induced cell death, ghost cells, cells membrane damage, and binucleated cells. The seed extract was found to have a higher cytotoxic and genotoxic effects than the leaf extract. These results suggest that the leaf and seeds extract of Telfairia occidentalis possess cytotoxic and genotoxic effects on A. cepa.","PeriodicalId":15398,"journal":{"name":"Journal of Complementary and Alternative Medical Research","volume":"89 S1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140236540","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-03-16DOI: 10.9734/jocamr/2024/v25i3520
Deni Yentipa
The factors that influence the nutritional status of children under five are very substantial to be studied. This aims to find out what factors are related so that power action and efforts to improve nutrition can be taken in the community. Because health is a form of human right which is explicitly mandated by the 1945 Constitution. Education and time for mothers and the health of parents, especially mothers, are one of the causes of malnutrition in toddlers. This study aims to determine the relationship between maternal characteristics and the nutritional status of children under five at the Suka Mulia Public Health Center, Nagan Raya Regency. The research procedure was by measuring body weight, mothers of children under five were given a nutritional status questionnaire. Data analysis using the Chi-square test showed that from 45 samples. The majority of children's nutritional status is good (77.8%). There was no relationship between maternal age and nutritional status of children under five at the Suka Mulia Public Health Center, Nagan Raya Regency (p=0.519). There is a relationship between mother's knowledge and nutritional status of children under five at the Suka Mulia Public Health Center, Nagan Raya Regency (p = 0.001). There is a relationship between maternal education and the nutritional status of children under five at the Suka Mulia Health Center, Nagan Raya Regency (p = 0.005). There is no relationship between mother's work and the nutritional status of children under five at the Suka Mulia Public Health Center, Nagan Raya Regency (p=0.070). There is a relationship between maternal parity and the nutritional status of children under five at the Suka Mulia Public Health Center, Nagan Raya Regency (p=0.003). From the results of the study, it can be concluded that knowledge, occupation, education and parity of mothers are related to the nutritional status of children under five. It is recommended that all mothers continue to seek information about children's health, especially the procedure for providing nutritious food to toddlers. "
{"title":"Relationship of Characteristics of Mothers and Nutritional Status of Tolls in Puskesmas Suka Mulia Nagan Raya District","authors":"Deni Yentipa","doi":"10.9734/jocamr/2024/v25i3520","DOIUrl":"https://doi.org/10.9734/jocamr/2024/v25i3520","url":null,"abstract":"The factors that influence the nutritional status of children under five are very substantial to be studied. This aims to find out what factors are related so that power action and efforts to improve nutrition can be taken in the community. Because health is a form of human right which is explicitly mandated by the 1945 Constitution. Education and time for mothers and the health of parents, especially mothers, are one of the causes of malnutrition in toddlers. This study aims to determine the relationship between maternal characteristics and the nutritional status of children under five at the Suka Mulia Public Health Center, Nagan Raya Regency. The research procedure was by measuring body weight, mothers of children under five were given a nutritional status questionnaire. Data analysis using the Chi-square test showed that from 45 samples. The majority of children's nutritional status is good (77.8%). There was no relationship between maternal age and nutritional status of children under five at the Suka Mulia Public Health Center, Nagan Raya Regency (p=0.519). There is a relationship between mother's knowledge and nutritional status of children under five at the Suka Mulia Public Health Center, Nagan Raya Regency (p = 0.001). There is a relationship between maternal education and the nutritional status of children under five at the Suka Mulia Health Center, Nagan Raya Regency (p = 0.005). There is no relationship between mother's work and the nutritional status of children under five at the Suka Mulia Public Health Center, Nagan Raya Regency (p=0.070). There is a relationship between maternal parity and the nutritional status of children under five at the Suka Mulia Public Health Center, Nagan Raya Regency (p=0.003). From the results of the study, it can be concluded that knowledge, occupation, education and parity of mothers are related to the nutritional status of children under five. It is recommended that all mothers continue to seek information about children's health, especially the procedure for providing nutritious food to toddlers. \"","PeriodicalId":15398,"journal":{"name":"Journal of Complementary and Alternative Medical Research","volume":"66 5","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140236816","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-03-12DOI: 10.9734/jocamr/2024/v25i2519
Marie Victoria Eyog Nkolo, Mbassi Awa Hubert Désiré, E. T. Fokunang, C. Fokunang
Background: Camphor and eucalyptus essential oils contain camphor and eucalyptol respectively which serve as active ingredients in over-the-counter drugs to manage upper respiratory tract infections and pain in children. However, because of their pro-convulsant properties, seizures constitute a documented complication of their toxicity after ingestion, inhalation, and dermal exposure. Objectives: This study aimed to assess the knowledge, attitudes and practices of parents and health personnel on the risk of onset of seizures in children exposed to pharmaceutical products containing camphor and/or eucalyptus essential oils. Methods: This was a hospital-based cross-sectional study conducted in the Mother and Child Centre of Chantal Biya Foundation, a reference hospital. The survey was conducted using questionnaires directed to health personnel and parents of hospitalized children under 8 years old. Results: A total of 350 parents out of the 360 encountered were included in the study and 57 health personnel out of the 100 encountered. Parents 337 (96.3%) and health personnel 18 (31.6%) had poor knowledge on the issue. Parents 338 (96.6%) and health personnel 54 (94.75%) had very satisfactory attitude scores. Both parents 319 (91.1%) and health personnel 31 (54.4%) had harmful practices scores. Conclusion: This study showed that both parents and health personnel had poor knowledge, very satisfactory attitudes and harmful practices towards the risk of onset of seizures in children exposed to pharmaceutical products containing camphor and/or eucalyptus essential oils.
{"title":"Knowledge, Attitudes and Practices on Exposure to Camphor & Eucalyptus Essential Oils and Risk of Seizure Disorder in Children: A Cross Sectional Study","authors":"Marie Victoria Eyog Nkolo, Mbassi Awa Hubert Désiré, E. T. Fokunang, C. Fokunang","doi":"10.9734/jocamr/2024/v25i2519","DOIUrl":"https://doi.org/10.9734/jocamr/2024/v25i2519","url":null,"abstract":"Background: Camphor and eucalyptus essential oils contain camphor and eucalyptol respectively which serve as active ingredients in over-the-counter drugs to manage upper respiratory tract infections and pain in children. However, because of their pro-convulsant properties, seizures constitute a documented complication of their toxicity after ingestion, inhalation, and dermal exposure. \u0000Objectives: This study aimed to assess the knowledge, attitudes and practices of parents and health personnel on the risk of onset of seizures in children exposed to pharmaceutical products containing camphor and/or eucalyptus essential oils. \u0000Methods: This was a hospital-based cross-sectional study conducted in the Mother and Child Centre of Chantal Biya Foundation, a reference hospital. The survey was conducted using questionnaires directed to health personnel and parents of hospitalized children under 8 years old. \u0000Results: A total of 350 parents out of the 360 encountered were included in the study and 57 health personnel out of the 100 encountered. Parents 337 (96.3%) and health personnel 18 (31.6%) had poor knowledge on the issue. Parents 338 (96.6%) and health personnel 54 (94.75%) had very satisfactory attitude scores. Both parents 319 (91.1%) and health personnel 31 (54.4%) had harmful practices scores. \u0000Conclusion: This study showed that both parents and health personnel had poor knowledge, very satisfactory attitudes and harmful practices towards the risk of onset of seizures in children exposed to pharmaceutical products containing camphor and/or eucalyptus essential oils.","PeriodicalId":15398,"journal":{"name":"Journal of Complementary and Alternative Medical Research","volume":"47 2","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140249244","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-03-04DOI: 10.9734/jocamr/2024/v25i2518
DI Izunwanne, SK Mobisson, C. Nwafor, IC Ifedi, CI Onwukaike, HA Izunwanne
Introduction: It is generally believed that the presence of some neuroactive phytochemicals such as acetylcholine, histamine, serotonin, tannins etc. in Urtica dioica may yet possess the key to the management of certain neurological and behavioral conditions at an easily accessible and affordable rates. The growing acceptance of alternative medicine in Nigeria and the world at large has necessitated this research work so as to ensure that the general/consuming public is properly guided. Objective: This study was carried out to investigate the effect of administration of Urtica dioica on locomotor behavior. The amount of Urtica dioica reaching the Central Nervous System (CNS) is affected by the extent to which it is converted/stimulates any of the excitatory agents like acetylcholine in the periphery. Methods: A total of 30 apparently healthy Swiss male albino mice weighing 20-25g were used in the study. After the 14 days of acclimatization, the mice were randomly separated into three (3) groups of ten (10) animals each and were housed in separate cages. The amice in group one (1) serves as the control for the research work and were given water and normal animal feed. Group two (2) of the mice were given feed mixed 400mg/kg of the extract while group three (3) mice were given feed mixed with 800 mg/kg of the extract. The open field maze was employed for the evaluation of locomotor behavior while the extracts were mixed with the animals’ feed. So, the extracts were administered to the mice orally. The extracts were administered for a duration of 28days (4 weeks). Results: The frequency of rearing in the open field was significantly increased in the Urtica dioica fed group compared to control (p<0.05). There is also a significant increase in the frequency of Line Crosses, Centre Square Entry and Rearing in the test groups compared to control (p<0.01). At a higher dosage, the Frequency and Duration of Grooming significantly increased (p<0.05). The Frequency of Defecation also significantly increased (p<0.05). Conclusion: These results indicate that repeated administration of Urtica dioica enhances locomotory behavior in mice.
{"title":"Excitatory Effect of Urtica dioica on Locomotor Behaviour of Mice Using the Open Field Maze Task","authors":"DI Izunwanne, SK Mobisson, C. Nwafor, IC Ifedi, CI Onwukaike, HA Izunwanne","doi":"10.9734/jocamr/2024/v25i2518","DOIUrl":"https://doi.org/10.9734/jocamr/2024/v25i2518","url":null,"abstract":"Introduction: It is generally believed that the presence of some neuroactive phytochemicals such as acetylcholine, histamine, serotonin, tannins etc. in Urtica dioica may yet possess the key to the management of certain neurological and behavioral conditions at an easily accessible and affordable rates. The growing acceptance of alternative medicine in Nigeria and the world at large has necessitated this research work so as to ensure that the general/consuming public is properly guided. \u0000Objective: This study was carried out to investigate the effect of administration of Urtica dioica on locomotor behavior. The amount of Urtica dioica reaching the Central Nervous System (CNS) is affected by the extent to which it is converted/stimulates any of the excitatory agents like acetylcholine in the periphery. \u0000Methods: A total of 30 apparently healthy Swiss male albino mice weighing 20-25g were used in the study. After the 14 days of acclimatization, the mice were randomly separated into three (3) groups of ten (10) animals each and were housed in separate cages. The amice in group one (1) serves as the control for the research work and were given water and normal animal feed. Group two (2) of the mice were given feed mixed 400mg/kg of the extract while group three (3) mice were given feed mixed with 800 mg/kg of the extract. The open field maze was employed for the evaluation of locomotor behavior while the extracts were mixed with the animals’ feed. So, the extracts were administered to the mice orally. The extracts were administered for a duration of 28days (4 weeks). \u0000Results: The frequency of rearing in the open field was significantly increased in the Urtica dioica fed group compared to control (p<0.05). There is also a significant increase in the frequency of Line Crosses, Centre Square Entry and Rearing in the test groups compared to control (p<0.01). At a higher dosage, the Frequency and Duration of Grooming significantly increased (p<0.05). The Frequency of Defecation also significantly increased (p<0.05). \u0000Conclusion: These results indicate that repeated administration of Urtica dioica enhances locomotory behavior in mice.","PeriodicalId":15398,"journal":{"name":"Journal of Complementary and Alternative Medical Research","volume":"39 10","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140080821","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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