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PA0575 (RmcA) interacts with other c-di-GMP metabolizing proteins in Pseudomonas aeruginosa PAO1. PA0575 (RmcA)与铜绿假单胞菌PAO1中其他c-二gmp代谢蛋白相互作用。
IF 1.2 4区 生物学 Q2 Medicine Pub Date : 2023-01-24 DOI: 10.2323/jgam.2022.05.003
Yanxiang Yao, Naren Xi, E Hai, Xiaomin Zhang, Jiayi Guo, Zhi Lin, Weidong Huang

As a central signaling molecule, c-di-GMP (bis-(3,5)-cyclic diguanosine monophosphate) is becoming the focus for research in bacteria physiology. Pseudomonas aeruginosa PAO1 genome contains highly complicated c-di-GMP metabolizing genes and a number of these proteins have been identified and investigated. Especially, a sophisticated network of these proteins is emerging. In current study, mainly through Bacteria-2-Hybrid assay, we found PA0575 (RmcA), a GGDEF-EAL dual protein, to interact with two other dual proteins of PA4601 (MorA) and PA4959 (FimX). These observations imply the intricacy of c-di-GMP metabolizing protein interactions. Our work thus provides one piece of data to increase the understandings to c-di-GMP signaling.

c-二- gmp(双-(3,5)-环二鸟苷单磷酸)作为一种中心信号分子正成为细菌生理学研究的热点。铜绿假单胞菌PAO1基因组包含高度复杂的c-二gmp代谢基因,许多这些蛋白质已经被鉴定和研究。特别是,这些蛋白质的复杂网络正在出现。在本研究中,我们主要通过Bacteria-2-Hybrid实验,发现ggdefa - eal双蛋白PA0575 (RmcA)与另外两个双蛋白PA4601 (MorA)和PA4959 (FimX)相互作用。这些观察结果暗示了c-二gmp代谢蛋白相互作用的复杂性。因此,我们的工作为增加对c-di-GMP信号的理解提供了一条数据。
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引用次数: 0
Genotypic analysis of the FAS2-F1279Y (3836T>A) polymorphism conferring high ethyl caprylate productivity in industrial sake yeast strains. 清酒工业酵母菌高乙酸乙酯产率FAS2-F1279Y (3836T>A)多态性的基因型分析
IF 1.2 4区 生物学 Q2 Medicine Pub Date : 2023-01-24 DOI: 10.2323/jgam.2022.05.001
Takashi Kuribayashi, Toshiki Sakurai, Akira Hatakeyama, Toshio Joh, Mitsuoki Kaneoke

In Saccharomyces cerevisiae, ethyl caprylate is produced by the esterification of caprylic acid, which is synthesized through the action of fatty acid synthase. A recent study reported a yeast mutant with a single nucleotide substitution in the alpha subunit of fatty acid synthase (FAS2) gene (F1279Y; 3836T>A) that produced large amounts of ethyl caprylate. Here, we designed two primer sets (P1/P2 and P3/P4) with mismatches that incorporate restriction sites for the enzymes NdeI and SspI, respectively and developed an easy and rapid polymerase chain reaction-restriction fragment length polymorphism assay to identify yeasts harboring the FAS2-F1279Y mutation associated with high ethyl caprylate productivity.

在酿酒酵母菌中,辛酸是通过脂肪酸合酶的作用由辛酸酯化而产生辛酸乙酯。最近的一项研究报道了一种酵母突变体,其脂肪酸合成酶(FAS2)基因的α亚基(F1279Y;3836T>A)产生大量的辛酸乙酯。本研究设计了两组引物(P1/P2和P3/P4),分别包含NdeI酶和SspI酶的限制性内切位点,并建立了一种简单快速的聚合酶链反应-限制性片段长度多态性测定方法,以鉴定含有高羧酸乙酯产量相关的FAS2-F1279Y突变的酵母。
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引用次数: 0
Evaluation of dye decolorization using anaerobic granular sludge from an expanded granular sludge bed based on spectrometric and microbiome analyses. 基于光谱和微生物组分析的膨胀颗粒污泥床厌氧颗粒污泥染料脱色评价。
IF 1.2 4区 生物学 Q2 Medicine Pub Date : 2023-01-24 DOI: 10.2323/jgam.2022.04.003
Tomohiro Inaba, Mami Yamaguchi, Akira Taniguchi, Yuya Sato, Tomo Aoyagi, Tomohiro Hori, Hiroyuki Inoue, Masahiko Fujita, Masanori Iwata, Yoshihiro Iwata, Hiroshi Habe

The decolorization of 11 dyes by granular sludge from an anaerobic expanded granular sludge bed (EGSB) reactor was evaluated. Biological decolorization of Reactive Red 21, 23, and 180, and Reactive Yellow 15, 17, and 23 in model textile wastewater was observed for the first time after a 7-day incubation (over 94% decolorization). According to the sequencing analysis of 16S rRNA gene amplicons from EGSB granular sludge, the operational taxonomic unit related to Paludibacter propionicigenes showed the highest increase in relative abundance ratios in the presence of dyes (7.12 times on average over 11 dyes) compared to those without dyes.

研究了厌氧膨胀颗粒污泥床(EGSB)反应器中颗粒污泥对11种染料的脱色效果。经过7天的培养,首次观察到活性红21、23、180和活性黄15、17、23在模型纺织废水中的生物脱色效果(脱色率超过94%)。根据对EGSB颗粒污泥16S rRNA基因扩增子的测序分析,与丙酸古杆菌相关的操作分类单元在染料存在时的相对丰度比在没有染料的情况下增加最多(平均为7.12倍)。
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引用次数: 0
Influence of polyamine production and proteolytic activities of co-cultivated bacteria on histamine production by Morganiella morganii. 共培养细菌多胺产量和蛋白水解活性对摩根氏摩根氏菌组胺产量的影响。
IF 1.2 4区 生物学 Q2 Medicine Pub Date : 2023-01-24 DOI: 10.2323/jgam.2022.04.001
Suma Devivilla, Manjusha Lekshmi, Fathima Salam, Sanath Kumar H, Rajendran Kooloth Valappil, Sibnarayan Dam Roy, Binaya Bhusan Nayak

Consumption of temperature-abused marine fish containing elevated levels of histamine results in histamine poisoning. Histamine is a biogenic amine produced in fish by the action of certain groups of bacteria which are capable of producing an exogenous enzyme called histidine decarboxylase (HDC). Morganella morganii is one of the major causative organisms of histamine poisoning. In this study, the histamine forming potential of M. morganii (BSS142) was evaluated when it was co-incubated with proteolytic as well as polyamine forming bacteria. This experiment was designed to examine whether biotic factors such as proteolysis and the presence of other amines influenced histamine forming ability of BSS142. The study showed that the proteolytic activity of Aeromonas hydrophila as well as Pseudomonas aeruginosa greatly enhanced the histamine forming ability of M. morganii. Psychrobacter sangunis, a non proteolytic polyamine producer, negatively influenced histamine production by M. morganii.

食用温度过高、组胺含量过高的海鱼会导致组胺中毒。组胺是鱼体内的一种生物胺,由某些细菌群的作用产生,这些细菌群能够产生一种叫做组氨酸脱羧酶(HDC)的外源酶。莫氏摩根菌是引起组胺中毒的主要病原菌之一。本研究对莫氏分枝杆菌(BSS142)与蛋白水解菌和多胺形成菌共培养时的组胺形成潜力进行了评价。本实验旨在研究生物因素如蛋白质水解和其他胺的存在是否影响BSS142的组胺形成能力。研究表明,嗜水气单胞菌和铜绿假单胞菌的蛋白水解活性大大增强了摩根氏分枝杆菌的组胺形成能力。非蛋白水解性多胺生产者桑格尼氏冻杆菌对莫氏分枝杆菌的组胺生产产生负面影响。
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引用次数: 0
Isolation and identification of psychrotrophic lactic acid bacteria in godo, the traditional fermented soy food in Japan. 日本传统豆制品腐乳中嗜冷性乳酸菌的分离鉴定。
IF 1.2 4区 生物学 Q2 Medicine Pub Date : 2023-01-24 DOI: 10.2323/jgam.2022.04.002
Kasumi Shimodate, Hiroyuki Honda

Godo is a traditional fermented soy food made in Aomori prefecture, Japan. It is mainly made of soybeans, rice koji, and salt. Since godo ripens during the long and severe winter in northeast Japan, it is assumed that lactic acid bacteria inhabiting godo have cold tolerance. We aimed to investigate the presence or absence of psychrotrophic lactic acid bacteria in godo. The viable counts of estimated lactic acid bacteria ranged from 106 to 108 cfu/g. In addition, aerobic and anaerobic microorganisms were detected in four godo products though the microbial population differed from sample to sample. Twenty-two bacterial strains were able to be isolated from godo, and all of the isolated strains were Gram-positive and catalase-negative. Some of the isolates grew well at 10°C. The carbohydrate fermentation profile of the selected three strains was determined by API50 CHL analysis. These strains were identified as Leuconostoc mesenteroides, and Latilactobacillus sakei by 16S rRNA gene sequence analysis. Leuconostoc mesenteroides strains HIT231 and HIT252, and Latilactobacillus sakei strain HIT273 could grow at 5°C in MRS broth, but their optimum growth temperature was 20°C-30°C. These results suggest that psychrotrophic lactic acid bacteria presumed to be derived from rice koji are present in godo, which is one of the factors in the low temperature ripening of godo in winter.

Godo是一种产自日本青森县的传统发酵豆制品。它主要由大豆、米曲和盐制成。由于日本东北部的鱼道在漫长而严酷的冬季成熟,因此人们认为栖息在鱼道上的乳酸菌具有耐寒性。我们的目的是调查godo中是否存在精神营养乳酸菌。估计的乳酸菌活菌数为106 ~ 108 cfu/g。此外,在四种godo产品中检测到好氧和厌氧微生物,但不同样品的微生物种群不同。从godo中分离出22株细菌,革兰氏阳性和过氧化氢酶阴性。部分菌株在10℃下生长良好。通过API50 CHL分析确定了所选菌株的碳水化合物发酵特征。经16S rRNA基因序列分析,这两株菌株分别为肠系膜Leuconostoc mesenteroides和sakei Latilactobacillus。肠系膜白孢杆菌HIT231、HIT252和saki乳酸杆菌HIT273在5℃的MRS肉汤中均能生长,但其最适生长温度为20℃~ 30℃。上述结果表明,果道中存在被推测为来源于米曲的心营养乳酸菌,这是果道冬季低温成熟的因素之一。
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引用次数: 1
Bacterial cellulose production from Komagataeibacter xylinus TISTR 1011 and Komagataeibacter nataicola TISTR 975 using yam bean juice as a nutrient source. 以山药豆汁为营养源的木林komagataeibacter xylinus TISTR 1011和Komagataeibacter nataicola TISTR 975细菌纤维素生产研究。
IF 1.2 4区 生物学 Q2 Medicine Pub Date : 2023-01-24 DOI: 10.2323/jgam.2022.05.002
Orn Anong Chaiyachet, Ketsara Wongtham, Komsan Sangkasame

The present study investigated the efficacy of bacterial cellulose production by K. xylinus TISTR 1011 and K. nataicola TISTR 975 using yam bean juice as a nutrient source, and the physicochemical and sensory characteristics of bacterial cellulose were examined. Bacterial cellulose content, production yield, and production rate were significantly higher when K. xylinus TISTR 1011 rather than K. nataicola TISTR 975 was used as the bacterial strain. The analysis of physicochemical characteristics revealed that bacterial cellulose produced by K. xylinus TISTR 1011 using yam bean juice medium had higher scores for CIE L*, a*, and b* values, wet weight, moisture content, firmness, and gel strength than bacterial cellulose produced by K. nataicola TISTR 975. In contrast, sensory evaluation showed that the acceptability scores and preference of all attributes of bacterial cellulose produced by K. nataicola TISTR 975 using yam bean juice medium were higher than those of bacterial cellulose produced by K. xylinus TISTR 1011. The results of this study indicate that yam bean juice from yam bean tubers, an alternative raw material agricultural product, can be used as a nutrient source for producing bacterial cellulose or nata by Komagataeibacter strains.

以山药豆汁为营养来源,研究了K. xylinus TISTR 1011和K. nataicola TISTR 975生产细菌纤维素的效果,并对细菌纤维素的理化特性和感官特性进行了研究。以K. xylinus TISTR 1011为菌株的细菌纤维素含量、产量和生产速率均显著高于K. nataicola TISTR 975。理化特性分析表明,在山芋豆汁培养基中,K. xylinus TISTR 1011生产的细菌纤维素在CIE L*、a*和b*值、湿重、含水量、硬度和凝胶强度等指标上均优于K. nataicola TISTR 975。感官评价结果表明,在山药豆汁培养基中,nataicola TISTR 975生产的细菌纤维素各属性的可接受性得分和偏好度均高于K. xylinus TISTR 1011。本研究结果表明,山药豆汁作为一种替代农产品原料,可以作为komagataeibacterium菌株生产细菌纤维素或nata的营养来源。
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引用次数: 1
The long journey of the Thermus thermophilus Whole-Cell project. 嗜热菌全细胞项目的漫长旅程。
IF 1.2 4区 生物学 Q2 Medicine Pub Date : 2023-01-01 DOI: 10.2323/jgam.2023.08.005
Yoshitaka Bessho
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引用次数: 0
Cloning, Expression, and Characterization of a GHF 11 Xylanase from Alteromonas macleodii HY35 in Escherichia coli. 大肠杆菌中异交单胞菌HY35中ghf11木聚糖酶的克隆、表达和特性研究
IF 1.2 4区 生物学 Q2 Medicine Pub Date : 2022-11-10 Epub Date: 2022-08-15 DOI: 10.2323/jgam.2021.10.003
Yanjie Tian, Jia Xu, Jianing Shi, Mengyuan Kong, Changjiang Guo, Caixia Cui, Yongtao Wang, Yan Wang, Chenyan Zhou

A xylanase gene xynZT-1 from Alteromonas macleodii HY35 was cloned and expressed in Escherichia coli (E. coli). The sequencing results showed that the ORF of xynZT-1 was 831 bp. The xylanase DNA sequence encoded a 29 amino acids (aa) signal peptide and a 247-aa mature peptide. The XynZT-1 has been a calculated molecular weight (MW) of 27.93 kDa, isoelectric point (pI) of 5.11 and the formula C1266H1829N327O384S5. The amino acid sequence of the xynZT-1 had a high similarity with that of glycosyl hydrolase family 11 (GHF11) reported from other microorganisms. The DNA sequence encoding mature peptide was subcloned into pET-28a(+) expression vector. The resulted plasmid pET-28a-xynZT-1 was transformed into E. coli BL21(DE3), and the recombinant strain BL21(DE3)/xynZT-1 was obtained. The optimum temperature and pH of the recombinant XynZT-1 were 45 ℃ and 5.0, respectively.

克隆了一株macleodii Alteromonas HY35的木聚糖酶基因xynZT-1,并在大肠杆菌中表达。测序结果显示,xynZT-1的ORF为831 bp。木聚糖酶DNA序列编码一个29个氨基酸的信号肽和一个247-aa的成熟肽。XynZT-1的分子量为27.93 kDa,等电点为5.11,分子式为C1266H1829N327O384S5。xynZT-1的氨基酸序列与其他微生物中报道的糖基水解酶家族11 (GHF11)的氨基酸序列高度相似。将编码成熟肽的DNA序列亚克隆到pET-28a(+)表达载体上。将得到的质粒pET-28a-xynZT-1转化到大肠杆菌BL21(DE3)中,得到重组菌株BL21(DE3)/xynZT-1。重组蛋白XynZT-1的最佳温度和pH分别为45℃和5.0。
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引用次数: 0
3-Amino-4-hydroxybenzoic acid production from glucose and/or xylose via recombinant Streptomyces lividans. 重组lividans链霉菌从葡萄糖和/或木糖生产3-氨基-4-羟基苯甲酸。
IF 1.2 4区 生物学 Q2 Medicine Pub Date : 2022-09-15 Epub Date: 2022-07-12 DOI: 10.2323/jgam.2022.06.001
Satoko Niimi-Nakamura, Hideo Kawaguchi, Kouji Uematsu, Hiroshi Teramura, Sachiko Nakamura-Tsuruta, Norimasa Kashiwagi, Yoshinori Sugai, Yohei Katsuyama, Yasuo Ohnishi, Chiaki Ogino, Akihiko Kondo

The aromatic compound 3-amino-4-hydroxybenzoic acid (3,4-AHBA) can be employed as a raw material for high-performance industrial plastics. The aim of this study is to produce 3,4-AHBA via a recombinant Streptomyces lividans strain containing griI and griH genes derived from Streptomyces griseus using culture medium with glucose and/or xylose, which are the main components in lignocellulosic biomass. Production of 3,4-AHBA by the recombinant S. lividans strain was successful, and the productivity was affected by the kind of sugar used as an additional carbon source. Metabolic profiles revealed that L aspartate-4-semialdehyde (ASA), a precursor of 3,4-AHBA, and coenzyme NADPH were supplied in greater amounts in xylose medium than in glucose medium. Moreover, cultivation in TSB medium with a mixed sugar (glucose/xylose) was found to be effective for 3,4-AHBA production, and optimal conditions for efficient production were designed by changing the ratio of glucose to xylose. The best productivity of 2.70 g/L was achieved using a sugar mixture of 25 g/L glucose and 25 g/L xylose, which was 1.5 times higher than the result using 50 g/L glucose alone. These results suggest that Streptomyces is a suitable candidate platform for 3,4-AHBA production from lignocellulosic biomass-derived sugars under appropriate culture conditions.

芳香族化合物3-氨基-4-羟基苯甲酸(3,4- ahba)可作为高性能工业塑料的原料。本研究的目的是利用含葡萄糖和/或木糖(木质纤维素生物质的主要成分)的培养基,利用从灰色链霉菌中提取的含有griI和griH基因的重组lividans链霉菌菌株生产3,4- ahba。重组S. lividans菌株成功生产了3,4- ahba,其产量受作为附加碳源的糖的种类的影响。代谢谱显示,木糖培养基比葡萄糖培养基提供更多的L -天冬氨酸-4-半醛(ASA), 3,4- ahba的前体和辅酶NADPH。此外,在混合糖(葡萄糖/木糖)的TSB培养基中培养3,4- ahba是有效的,并通过改变葡萄糖与木糖的比例设计了最优生产条件。25 g/L葡萄糖和25 g/L木糖混合的产率为2.70 g/L,比单独使用50 g/L葡萄糖的产率高1.5倍。这些结果表明,链霉菌是在适当的培养条件下从木质纤维素生物质来源的糖生产3,4- ahba的合适的候选平台。
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引用次数: 0
Functional analysis of a gene cluster for putative bacteriocin deduced from the genome sequence of Aeribacillus pallidus PI8. 苍白芽孢杆菌PI8基因组序列推测细菌素基因簇的功能分析。
IF 1.2 4区 生物学 Q2 Medicine Pub Date : 2022-09-15 DOI: 10.2323/jgam.2021.11.003
Kyosuke Kita, Sanako Yoshida, Shu Ishikawa, Ken-Ichi Yoshida

Bacteriocins are a large family of peptides synthesized ribosomally by a variety of bacterial species. The genome of one of the thermophilic Gram-positive bacteria, Aeribacillus pallidus PI8, was found to possess an operon comprising five genes possibly involved in the production of a putative bacteriocin that was named pcnABCDE for the production of "pallidocyclicin." This study investigated the function of the pcn operon experimentally. The heterologous expression of the entire pcn operon from the plasmid was toxic to Escherichia coli but not to Bacillus subtilis. However, when the entire pcn operon was expressed constitutively, even the growth of B. subtilis was impaired, and at least pcnA was implied to serve as the precursor of pallidocyclicin. In addition, a strain of B. subtilis expressing the entire pcn operon from the plasmid showed toxicity to another thermophilic species, Geobacillus kaustophilus, at elevated temperatures, whereas another strain lacking pcnE alone from the pcn operon lost the toxicity, suggesting that pcnE might be involved in the biosynthesis of pallidocyclicin when it is produced in B. subtilis.

细菌素是一大类由多种细菌核糖体合成的多肽。一种嗜热的革兰氏阳性细菌——苍白球芽孢杆菌PI8的基因组被发现拥有一个由五个基因组成的操纵子,可能与一种假定的细菌素的产生有关,这种细菌素被命名为pcnABCDE,用于生产“苍白球素”。本研究对pcn操纵子的功能进行了实验研究。从质粒中外源表达整个pcn操纵子对大肠杆菌有毒性,但对枯草芽孢杆菌无毒性。然而,当整个pcn操纵子组成表达时,即使枯草芽孢杆菌的生长也受到损害,至少pcnA可能是pallidocyclicin的前体。此外,一株从质粒中表达整个pcn操纵子的枯草芽孢杆菌在高温下对另一种嗜热物种kaustophilus Geobacillus显示出毒性,而另一株从pcn操纵子中单独缺乏pcnE的菌株则失去了毒性,这表明pcnE可能在枯草芽孢杆菌中产生时参与了苍白霉素的生物合成。
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引用次数: 0
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Journal of General and Applied Microbiology
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