Journal of Food Composition and Analysis最新文献_第8页

Engineering the hierarchical heterostructure of core-shell nanoparticles to improve SERS enhancement and cysteine detection 设计核壳纳米粒子的分层异质结构以提高SERS增强和半胱氨酸检测

IF 4.6 2区农林科学 Q2 CHEMISTRY, APPLIED

Journal of Food Composition and Analysis

Pub Date : 2026-01-16 DOI: 10.1016/j.jfca.2026.108910

Yuanfa Lai , Yanyu Zhang , Qihui Zhang , Zhiyong Guo

Cysteine (Cys), a conditionally essential amino acid in biological systems and widely utilized in the food industry. While dietary intake of cysteine-rich foods replenishes exogenous sources, aberrant levels can instigate pathological conditions. The scarcity of reliable methods for quantifying cysteine in food matrices underscores the urgent need for accurate and sensitive detection methodologies to safeguard food safety and human health. In our study, a self-assembly method is employed to construct a porous, highly cross-linked shell around Au NPs via the oxidation-polymerization reaction of Dopamine (DA) and N-aminoethyl piperazine propane sulfonate (AEPPS) at room temperature. The polymeric shell strengthens the interaction between the Au NPs and the DA-AEPPS layer via hydrogen and covalent bonds, and effectively mitigates Au NP aggregation. It can be applied to the quantification of Cys in food matrices with short detection times and high selectivity, enabling precise differentiation and measurement. The detection limit of this method reached 78.4 nmol/L, demonstrating high sensitivity and promising applications in the field of food safety. A series of Cys spiking recovery experiments is conducted using the present method and high-performance liquid chromatography (HPLC). While the Cys is commonly found in milk, apples, and flour, which selected as the analyte for testing in these food samples. The RSD of the present method with different spiking and samples is found range from 1.3 % to 4.4 %, with recoveries ranging from 95.1 % to 110.1 % (the HPLC method yielded a recovery rate of 86.3–111.7 %, with an RSD ranging from 1.2 % to 2.1 %). The adsorption and detection capabilities for Cys can be significantly enhanced through specific chemical interactions, providing an efficient means for food safety detection.

半胱氨酸（Cys）是生物系统中的一种条件必需氨基酸，在食品工业中得到广泛应用。虽然饮食摄入富含半胱氨酸的食物补充了外源来源，但异常水平可能引发病理状况。由于缺乏定量食品基质中半胱氨酸的可靠方法，因此迫切需要准确灵敏的检测方法，以保障食品安全和人类健康。在我们的研究中，采用自组装方法，在室温下通过多巴胺（DA）和n-氨基乙基哌嗪丙烷磺酸盐（AEPPS）的氧化聚合反应，在Au NPs周围构建了多孔的、高度交联的壳层。聚合物壳层通过氢键和共价键加强Au NP与DA-AEPPS层之间的相互作用，有效减轻Au NP的聚集。该方法可用于食品基质中Cys的定量，检测时间短，选择性高，可实现精确的区分和测量。该方法检出限为78.4 nmol/L，灵敏度高，在食品安全领域具有广阔的应用前景。采用本方法和高效液相色谱法对Cys进行了一系列的加峰回收率实验。而Cys通常存在于牛奶，苹果和面粉中，因此选择作为这些食品样品的分析物进行测试。不同峰和样品的RSD范围为1.3 % ~ 4.4 %，加样回收率为95.1% % ~ 110.1% %（高效液相色谱法加样回收率为86.3 ~ 111.7 %，RSD为1.2 % ~ 2.1 %）。通过特定的化学相互作用，可以显著增强对Cys的吸附和检测能力，为食品安全检测提供了一种有效的手段。

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引用次数: 0

Assessment of nutritional and functionality potential of Palak (Beta vulgaris var. bengalensis) germplasm: A mineral-rich Northern Indian traditional and underutilized green leafy vegetable 巴叻（Beta vulgaris var. bengalensis）种质的营养和功能潜力评估：一种富含矿物质的印度北部传统和未充分利用的绿叶蔬菜

IF 4.6 2区农林科学 Q2 CHEMISTRY, APPLIED

Journal of Food Composition and Analysis

Pub Date : 2026-01-15 DOI: 10.1016/j.jfca.2026.108892

Partik Sharma, Hira Singh, Vivek Sharma, Vikas Kumar, Monika Mahajan

The present investigation aimed to genetically evaluate palak (Beta vulgaris var. bengalensis) germplasm to identify nutritionally superior genotypes rich in essential minerals and bioactive compounds. Forty genetically diverse genotypes were biochemically profiled over two consecutive seasons. Genetic variability, heritability, and trait associations were assessed through parameter estimation, principal component analysis (PCA), correlation, and hierarchical clustering. Substantial diversity was observed for mineral nutrients (Zn, Cu, Fe, Mn, Mg, K, Na, S) and phytochemicals [total phenolic content (TPC), total flavonoid content (TFC), antioxidant activity (DPPH)]. Genotype PP35 showed maximum Mg (9751.33), Cu (17.60), and K (1476.23) in mg/kg, while PP36 exhibited highest Zn (86.83 mg/kg), P (9533.33 mg/kg), and Fe (829.47 mg/kg) concentrations with high inter-seasonal stability. PP46 and PP47 recorded > 93 % DPPH inhibition, and PP17 and PP7 showed > 430 mg GAE/100 g DW TPC. High heritability (>0.95) and genetic advance for Fe, TPC, TFC, S, P, and K indicated strong additive gene action. PCA revealed that PC1 and PC2 explained 43.5 % of total variation. Cluster analysis identified eight elite genotypes combining micronutrient density, antioxidant potential, and stability. These findings provide a genetic foundation for biofortification and functional food development to enhance nutritional security.

本研究旨在对白桦（Beta vulgaris var. bengalensis）种质资源进行遗传评价，以确定富含必需矿物质和生物活性化合物的营养优势基因型。在连续两个季节中对40种不同的基因型进行了生化分析。通过参数估计、主成分分析（PCA）、相关性和层次聚类来评估遗传变异性、遗传力和性状相关性。矿质养分（Zn、Cu、Fe、Mn、Mg、K、Na、S）和植物化学物质（总酚含量（TPC）、总黄酮含量（TFC）和抗氧化活性（DPPH））存在显著差异。基因型PP35的Mg（9751.33）、Cu（17.60）和K（1476.23）含量最高（Mg /kg），而PP36的Zn（86.83 Mg /kg）、P（9533.33 Mg /kg）和Fe（829.47 Mg /kg）含量最高，具有较高的季节间稳定性。PP46和PP47记录了>； 93 %的DPPH抑制，PP17和PP7显示>； 430 mg GAE/100 g DW TPC。铁、TPC、TFC、S、P、K具有较高的遗传力（>0.95）和遗传进展，说明加性基因作用强。PCA显示PC1和PC2解释了43.5 %的总变异。聚类分析鉴定出8个结合微量营养素密度、抗氧化潜力和稳定性的优良基因型。这些发现为生物强化和功能食品的开发提供了遗传学基础，以提高营养安全。

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引用次数: 0

Simultaneous estimation of Linalool and Eugenol in herbal formulations using a validated RP-HPLC method: A green analytical approach 有效的反相高效液相色谱法同时测定中药制剂中的芳樟醇和丁香酚：一种绿色分析方法

IF 4.6 2区农林科学 Q2 CHEMISTRY, APPLIED

Journal of Food Composition and Analysis

Pub Date : 2026-01-15 DOI: 10.1016/j.jfca.2026.108909

Ankita Das , Parixit Prajapati

Linalool and Eugenol commonly found in herbal products happen to be major bioactive components because of their anti-inflammatory, antioxidant, as well as antimicrobial activities. For the quality control and standardization of these products, a strong analytical technique that truly is effective enough is necessary. The current study develops and validates a reversed-phase high-performance liquid chromatography method that is sufficiently simple, accurate, and precise. The method quantifies combined amounts of Linalool and Eugenol in the herbal formulations. A C18 column that flowed at 1.0 mL/min separated chromatographically using acetonitrile and water mobile phase (55:45, %v/v). The approach was validated in accordance with ICH Q2(R2) guidelines for parameters like linearity, specificity, LOQ, LOD, robustness, accuracy, and precision. For both of the analytes with correlation coefficients greater than 0.9957, the technique demonstrated quite good linearity. This linearity was present between about 2–30 µg/mL. AGREE, GAPI, NEMI, tools were used to evaluate the method's greenness. Concepts of White Analytical Chemistry (WAC) were brought in using RGB fast, as well as BAGI models. Sustainability, safety, and environmental footprint, for each of the method were assessed. The method developed was successfully used in commercial herbal products, proving its applicability for routine quality control purposes.

在草药产品中常见的芳樟醇和丁香酚恰好是主要的生物活性成分，因为它们具有抗炎、抗氧化和抗菌活性。对于这些产品的质量控制和标准化，需要一个真正有效的强大的分析技术。本研究开发并验证了一种简单、准确、精确的反相高效液相色谱法。该方法定量了中药制剂中芳樟醇和丁香酚的组合量。色谱柱为C18，流速为1.0 mL/min，乙腈-水流动相（55:45,%v/v）。根据ICH Q2（R2）指南对线性、特异性、定量限、定量限、鲁棒性、准确性和精密度等参数进行了验证。对于相关系数大于0.9957的两种分析物，该技术表现出良好的线性。线性关系在2-30 µg/mL之间。采用AGREE、GAPI、NEMI等工具评价方法的绿色度。白色分析化学（WAC）的概念被引入使用RGB快速，以及BAGI模型。对每种方法的可持续性、安全性和环境足迹进行了评估。所建立的方法已成功地应用于商业草药产品中，证明了其在日常质量控制中的适用性。

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引用次数: 0

Yellow pea germination impact on protein isolation - protein digestibility and quality 黄豌豆发芽对蛋白质分离-蛋白质消化率和品质的影响

IF 4.6 2区农林科学 Q2 CHEMISTRY, APPLIED

Journal of Food Composition and Analysis

Pub Date : 2026-01-15 DOI: 10.1016/j.jfca.2026.108902

Anamaria Pop , Călina Ciont , Oana-Leila Pop , Dan-Cristian Vodnar , Răzvan Odocheanu , Elena Mudura , Georgiana Smaranda Marțiș , Sonia Ancuța Socaci , Gheorghe-Adrian Martău

Enhancing the amino acid composition and nutritional quality of plant-based protein ingredients is essential for sustainable food development. This study investigated the effects of germination on yellow pea (Pisum sativum L.) protein isolates using the micellar precipitation method. The results indicate that short-term germination induces compositional and structural modifications in yellow pea proteins, which persist through micellar precipitation and influence the amino acid profile of the resulting isolates. Germination activated endogenous proteolytic systems, leading to partial hydrolysis of storage globulins and a shift toward lower-molecular-weight peptides. Germinated isolates exhibited a lower loss of essential and non-essential amino acids during digestion, resulting in higher non-essential amino acid bioaccessibility (23 %) compared with non-germinated isolates (17 %). After digestion, germinated isolates showed reduced histidine and phenylalanine levels, reflecting increased digestive release of these amino acids. Glutamic acid content increased in germinated isolates compared to non-germinated ones, while glycine levels rose post-digestion in germinated samples, indicating improved bioaccessibility. These findings underscore the value of germination as a strategy to improve the amino acid composition and digestive bioaccessibility of pea protein isolates, reinforcing their nutritional relevance for plant-based protein applications.

提高植物性蛋白质成分的氨基酸组成和营养质量对可持续粮食发展至关重要。采用胶束沉淀法研究了黄豌豆（Pisum sativum L.）蛋白分离物萌发的影响。结果表明，短期萌发诱导黄豌豆蛋白的组成和结构变化，这些变化通过胶束沉淀持续存在，并影响分离物的氨基酸谱。发芽激活了内源性蛋白水解系统，导致储存球蛋白部分水解，并向低分子量肽转移。发芽菌株在消化过程中必需和非必需氨基酸的损失更低，与未发芽菌株（17 %）相比，非必需氨基酸的生物可及性更高（23 %）。消化后，发芽的分离物显示组氨酸和苯丙氨酸水平降低，反映了这些氨基酸的消化释放增加。与未萌发的菌株相比，萌发后的菌株谷氨酸含量增加，而消化后的菌株甘氨酸水平上升，表明生物可及性提高。这些发现强调了发芽作为一种改善豌豆分离蛋白氨基酸组成和消化生物可及性的策略的价值，加强了它们在植物蛋白应用中的营养相关性。

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引用次数: 0

A FRET-triggered probe for specific and interference-resistant detection of organophosphorus pesticides by composite Zr-MOFs 复合Zr-MOFs的fret触发探针特异性和抗干扰检测有机磷农药

IF 4.6 2区农林科学 Q2 CHEMISTRY, APPLIED

Journal of Food Composition and Analysis

Pub Date : 2026-01-14 DOI: 10.1016/j.jfca.2026.108901

Youjia Tian , Zhijie Wang , Mengyao Li , Xianwei Luo , Mingxin Yang , Jingru Yang , Bingbing Peng , Jinhong Jiang , Minjie Li , Jia Liu , Dingyu Hu , Juan Li

Organophosphorus pesticides (OPPs) posed significant risks to both ecological and human health due to their widespread use and toxicity. Traditional detection methods for OPPs are complex and require expensive equipment. In this study, a novel fluorescence-based detection strategy is proposed, using serine-based carbon quantum dots (CQDs) encapsulated in a Zr-based porphyrin metal-organic framework (MOF). This composite material exploits the triggering of fluorescence resonance energy transfer (FRET) between the CQDs and Zr-MOF for selective detection of OPPs. The FRET efficiency of this system is 72.9 %, enabling it to have interference-resistant detection capabilities. The detection limits are approaching the detection limit of gas chromatography (∼5 µg/L). The probe is applicable for environmental monitoring and food safety testing, offering a promising alternative for rapid on-site detection of OPPs without relying on enzymes.

有机磷农药由于其广泛使用和毒性，对生态和人类健康构成重大风险。传统的opp检测方法复杂且需要昂贵的设备。在这项研究中，提出了一种新的基于荧光的检测策略，使用丝氨酸基碳量子点（CQDs）封装在基于zr的卟啉金属有机框架（MOF）中。该复合材料利用CQDs和Zr-MOF之间的荧光共振能量转移（FRET）触发选择性检测opp。该系统的FRET效率为72.9 %，具有抗干扰检测能力。检出限接近气相色谱法的检出限（~ 5 µg/L）。该探针适用于环境监测和食品安全检测，为不依赖于酶的OPPs快速现场检测提供了一种有前景的替代方案。

{"title":"A FRET-triggered probe for specific and interference-resistant detection of organophosphorus pesticides by composite Zr-MOFs","authors":"Youjia Tian , Zhijie Wang , Mengyao Li , Xianwei Luo , Mingxin Yang , Jingru Yang , Bingbing Peng , Jinhong Jiang , Minjie Li , Jia Liu , Dingyu Hu , Juan Li","doi":"10.1016/j.jfca.2026.108901","DOIUrl":"10.1016/j.jfca.2026.108901","url":null,"abstract":"<div><div>Organophosphorus pesticides (OPPs) posed significant risks to both ecological and human health due to their widespread use and toxicity. Traditional detection methods for OPPs are complex and require expensive equipment. In this study, a novel fluorescence-based detection strategy is proposed, using serine-based carbon quantum dots (CQDs) encapsulated in a Zr-based porphyrin metal-organic framework (MOF). This composite material exploits the triggering of fluorescence resonance energy transfer (FRET) between the CQDs and Zr-MOF for selective detection of OPPs. The FRET efficiency of this system is 72.9 %, enabling it to have interference-resistant detection capabilities. The detection limits are approaching the detection limit of gas chromatography (∼5 µg/L). The probe is applicable for environmental monitoring and food safety testing, offering a promising alternative for rapid on-site detection of OPPs without relying on enzymes.</div></div>","PeriodicalId":15867,"journal":{"name":"Journal of Food Composition and Analysis","volume":"150 ","pages":"Article 108901"},"PeriodicalIF":4.6,"publicationDate":"2026-01-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145978064","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Exploring the limits of UHPLC-MS/MS with polarity switching towards the quantification of 931 mycotoxins and other secondary metabolites in cereal-based food 探讨极性切换UHPLC-MS/MS在谷物食品中931种真菌毒素及其他次生代谢产物定量分析中的局限性

IF 4.6 2区农林科学 Q2 CHEMISTRY, APPLIED

Journal of Food Composition and Analysis

Pub Date : 2026-01-14 DOI: 10.1016/j.jfca.2026.108908

Lidija Kenjeric , Michael Sulyok , Christoph Bueschl , Alexandra Malachova , Rudolf Krska

High-throughput LC–MS/MS methods are increasingly essential for regulatory screening of mycotoxins in food as the number of relevant analytes grows. We evaluated the limits of such a quantitative methods covering 931 mycotoxins and other secondary metabolites in cereal-based food (muesli), by comparing ultra-high-performance liquid chromatography (UHPLC) with high-performance liquid chromatography (HPLC), polarity switching with separate injections, and short vs. long gradients to optimize speed without compromising performance. To manage validation workload, 52 representative analytes—including all regulated mycotoxins—distributed across the chromatogram were selected for assessment. The final multi-class LC–MS/MS quantitative method covers 931 mycotoxins and secondary metabolites in an 11-minute run time, providing a scalable solution for routine testing, regulatory and emergency response. Across methods, no significant differences were observed in matrix effects, repeatability, or within-laboratory reproducibility, except at the lowest spiking levels. Reduced repeatability increased limits of quantification (LOQs) by a factor of 3–4 compared to the original 2 × 20.5-minute method, highlighting the main trade-off when accelerating analysis, despite the nearly fourfold gain in throughput. This ultra-fast approach meets the required sensitivity and measurement uncertainty for all 14 regulated mycotoxins across five muesli varieties, supporting regulatory control. Replacing the conventional dual-injection workflow with this single-injection protocol could reduce laboratory CO₂ emissions by ∼22 tons annually.

随着相关分析物数量的增加，高通量LC-MS /MS方法在食品中真菌毒素的监管筛选中越来越重要。我们通过比较超高效液相色谱法（UHPLC）和高效液相色谱法（HPLC）、分离注射极性切换、短梯度和长梯度来优化速度而不影响性能，评估了该定量方法覆盖谷物食品（muesli）中931种真菌毒素和其他次级代谢物的局限性。为了管理验证工作量，选择分布在色谱图上的52个代表性分析物（包括所有受管制的霉菌毒素）进行评估。最终的多类别LC-MS /MS定量方法在11分钟的运行时间内涵盖931种真菌毒素和次级代谢物，为常规检测、监管和应急响应提供了可扩展的解决方案。在不同的方法中，除了最低峰值水平外，在基质效应、可重复性或实验室内可重复性方面没有观察到显著差异。与最初的2 × 20.5分钟方法相比，重复性降低使定量限（loq）增加了3-4倍，尽管吞吐量增加了近4倍，但在加速分析时突出了主要的权衡。这种超快速方法满足5个什米粥品种中所有14种受管制真菌毒素的灵敏度和测量不确定度要求，支持监管控制。用这种单次注射方案取代传统的双次注射工作流程，每年可减少实验室二氧化碳排放量约22吨。

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引用次数: 0

Effects of sulfuring, drying and storage on the formation of α-dicarbonyl compounds and 5-hydroxymethylfurfural in dried apricots 硫化、干燥和贮藏对杏干中α-二羰基化合物和5-羟甲基糠醛形成的影响

IF 4.6 2区农林科学 Q2 CHEMISTRY, APPLIED

Journal of Food Composition and Analysis

Pub Date : 2026-01-14 DOI: 10.1016/j.jfca.2026.108904

Işıl Gürsul Aktağ , Ezgi Doğan Cömert , Vural Gökmen

This study investigated the impact of sulfuring, various drying methods, and storage with different packaging on the formation of α-dicarbonyl compounds and 5-hydroxymethylfurfural in dried apricots. Changes in the concentrations of α-dicarbonyl compounds, 5-hydroxymethylfurfural, sugars, and color were monitored in the samples (sulfured/non-sulfured, oven-/sun-dried, polyethylene/vacuum/modified atmosphere packaging) during storage at room temperature for 12 months. Among α-dicarbonyl compounds, 3-deoxyglucosone was the most prevalent, reaching 1356.8 ± 70.6 mg/kg dry matter in sulfured oven-dried apricots after drying. Methylglyoxal reached its peak at 723.2 ± 9.9 mg/kg dry matter in sulfured sun-dried apricots after storage. 5-Hydroxymethylfurfural remained at significantly lower levels than the dominant α-dicarbonyl compounds. Both α-dicarbonyl compounds and 5-hydroxymethylfurfural increased significantly during storage, irrespective of packaging, while sulfuring influenced the type of α-dicarbonyl compounds formed. These findings underscore the significance of processing and storage in influencing α-dicarbonyl compounds accumulation, providing valuable insights into maintaining the safety and quality of dried apricots.

研究了硫化、不同干燥方式和不同包装对杏脯中α-二羰基化合物和5-羟甲基糠醛形成的影响。在室温下存放12个月的样品（含硫/无硫、烘箱/晒干、聚乙烯/真空/改性气氛包装）中，监测α-二羰基化合物、5-羟甲基糠醛、糖和颜色的浓度变化。在α-二羰基化合物中，以3-脱氧葡萄糖酮含量最高，干燥后硫化杏干物质含量达到1356.8 ± 70.6 mg/kg。含硫晒干杏中甲基乙二醛含量最高，为723.2 ± 9.9 mg/kg。5-羟甲基糠醛的含量明显低于α-二羰基化合物。无论包装如何，α-二羰基化合物和5-羟甲基糠醛在储存过程中均显著增加，而硫化影响α-二羰基化合物形成的类型。这些发现强调了加工和储存对α-二羰基化合物积累的影响，为保持杏干的安全和质量提供了有价值的见解。

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引用次数: 0

Evaluating protection effects of packaging and storage environment on Goji berry in the rural market of native cultivated zone with HS-SPME-GCMS combined with AntDAS-GCMS 采用HS-SPME-GCMS联合AntDAS-GCMS评价乡土产区农村市场枸杞包装与贮藏环境的保护效果

IF 4.6 2区农林科学 Q2 CHEMISTRY, APPLIED

Journal of Food Composition and Analysis

Pub Date : 2026-01-14 DOI: 10.1016/j.jfca.2026.108907

Wan-Ting Zou , Long-He Wang , Mei-Juan Fan , Guo-Bi Chai , Shu-Fang Li , Ping-Ping Liu , Hai-Yan Fu , Yong-Jie Yu , Yuanbin She

The protective effects of packaging and storage environment on the quality of Goji berries in rural markets were still unclear. To address this problem, we developed a systematic approach for comprehensively characterizing volatile organic compounds in Goji berries by utilizing headspace solid-phase microextraction gas chromatography spectrometry combined with AntDAS-GCMS. After a 30-day storage, a total of 201 differential components were screened between indoor and outdoor storage environments, among which 29 compounds were identified. The untargeted analysis suggested that the factor of storage environment had a more significant impact than the packaging. A further analysis of packaging under the indoor environment obtained 209 differential components, with 26 compounds identified. Results indicated that both the PE plastic jar and the PS hard plastic jar exhibited slightly better protection capabilities than the other packaging. Our research may emphasize the protection techniques for Goji berries in rural markets.

包装和储存环境对农村市场枸杞品质的保护作用尚不清楚。为了解决这一问题，我们开发了一种顶空固相微萃取气相色谱法结合AntDAS-GCMS对枸杞中挥发性有机化合物进行综合表征的系统方法。贮藏30 d后，共筛选出201个室内与室外贮藏环境差异成分，鉴定出29个化合物。非目标分析表明，储存环境因素的影响比包装因素更显著。在室内环境下对包装进行进一步分析，获得209种不同成分，鉴定出26种化合物。结果表明，PE塑料罐和PS硬塑料罐的保护能力略好于其他包装。我们的研究可以强调农村市场枸杞的保护技术。

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引用次数: 0

Comparison of NIR spectroscopy devices (Portable and Benchtop) and a low-cost E-nose for classification of black tea: A machine learning approach using PLSDA, LDA, and PCA 近红外光谱设备（便携式和台式）与低成本电子鼻用于红茶分类的比较：使用PLSDA， LDA和PCA的机器学习方法

IF 4.6 2区农林科学 Q2 CHEMISTRY, APPLIED

Journal of Food Composition and Analysis

Pub Date : 2026-01-14 DOI: 10.1016/j.jfca.2026.108898

Marcus Vinicius da Silva Ferreira , Jose Lucena Barbosa Jr , Douglas Fernandes Barbin , Mohammed kamruzzaman

Black tea adulteration is a widespread practice, involving the mixing or substitution of high-quality tea leaves with lower quality samples. The authentication of black tea regarding its composition and origin is crucial to ensure quality and maintain consumer trust. However, conventional methods (e.g., High-Performance Liquid Chromatography HPLC) for tea analysis are expensive, demand chemicals, and generate waste. This study evaluates two NIR instruments (portable (PNIR) and benchtop (BNIR)) and low-cost electronic nose (LC-e-nose) for classification of tea leaves from three different origins: Brazil (BR), United States (US), and India (IND). Principal Component Analysis (PCA), Linear Discriminant Analysis (LDA), and Partial Least Squares Discriminant Analysis (PLS-DA) were applied for data treatment, and the results demonstrate that the LC-e-nose exhibits F1 scores above 99 % for all models, comparable to NIR systems (F1 > 99 %). These findings highlight its potential as a cost-effective and reliable alternative to NIR spectroscopy for investigating tea quality and origin. By comparing these advanced techniques, this study provides an evaluation of black tea quality and authenticity at a significantly reduced cost.

红茶掺假是一种普遍的做法，涉及用低质量的样品混合或替代高质量的茶叶。红茶的成分和原产地认证对于确保质量和保持消费者的信任至关重要。然而，传统的茶叶分析方法（如高效液相色谱法）价格昂贵，需要化学物质，而且会产生废物。本研究评估了两种近红外仪器（便携式（PNIR）和台式（BNIR））和低成本电子鼻（lc -e-鼻）用于三个不同产地的茶叶分类：巴西（BR），美国（US）和印度（IND）。采用主成分分析（PCA）、线性判别分析（LDA）和偏最小二乘判别分析（PLS-DA）进行数据处理，结果表明，LC-e-nose在所有模型上的F1得分都在99 %以上，与NIR系统（F1 > 99 %）相当。这些发现突出了它作为一种具有成本效益和可靠的替代近红外光谱研究茶叶质量和原产地的潜力。通过对这些先进技术的比较，本研究以显著降低的成本提供了红茶质量和真实性的评估。

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引用次数: 0

Nutritional and safety assessment of brown seaweed species and their extracts 褐藻及其提取物的营养和安全性评价

IF 4.6 2区农林科学 Q2 CHEMISTRY, APPLIED

Journal of Food Composition and Analysis

Pub Date : 2026-01-13 DOI: 10.1016/j.jfca.2026.108900

Aurora Silva , Cristina Soares , Maria Carpena , Virginia Cruz Fernandes , Franklin Chamorro , Cristina Delerue-Matos , M. Fátima Barroso , M.A. Prieto

This study examines the nutritional profiles of Bifurcaria bifurcata (BB), Ascophyllum nodosum (AN), and Fucus spiralis (FS), as well as their extracts obtained through microwave-assisted extraction. Compositional analysis covered residual water, volatiles, fixed carbon, ash, lipids, sugars, protein, and fiber. Proximate analysis revealed high protein levels in FS (9.72 %) and BB (ext) (4.95 %). Lipid content ranged from 1.26 % to 4.49 %, and fiber content averaged 52.3 %, with FS peaking at 60.2 %. The study applied indices of atherogenicity (AI), thrombogenicity (TI), and the hypocholesterolemic/hypercholesterolemic (HH) ratio to assess the cardiovascular impact, with FS exhibiting the healthiest fatty acid combination. Amino acid analysis revealed the presence of all essential amino acids except phenylalanine. Taurine, beneficial for cardiovascular health and scarce in plant sources, was found to be significantly present in BB. Additionally, the presence of polyphenols was assessed by HPLC-MS/MS. It revealed a panoply of phenolic compounds, with phlorotannins accounting for more than 50 % of the compounds detected. Acceptable risk levels were confirmed by Estimated daily intake (EDI), target hazard quotient (THQ), and total cancer risk (TCR) analyses, even when considering moderate values of inorganic arsenic. The results highlight the nutritional value and safety of these macroalgae, emphasizing their potential applications in food.

本研究考察了双歧双歧菌（BB）、瘤状葡萄曲霉（AN）和螺旋藻（FS）的营养成分，以及它们的微波萃取提取物。成分分析包括残余水、挥发物、固定碳、灰分、脂类、糖、蛋白质和纤维。近似分析显示，FS（9.72 %）和BB (ext)（4.95 %）的蛋白水平较高。脂质含量为1.26 % ~ 4.49 %，纤维含量平均为52.3% %，FS最高为60.2 %。该研究应用动脉粥样硬化性（AI）、血栓形成性（TI）和低胆固醇血症/高胆固醇血症（HH）比值指标来评估心血管影响，FS表现出最健康的脂肪酸组合。氨基酸分析显示除苯丙氨酸外所有必需氨基酸均存在。牛磺酸有益于心血管健康，在植物来源中很少见，但在BB中发现了大量的牛磺酸。此外，采用高效液相色谱-质谱联用（HPLC-MS/MS）法测定样品中多酚的含量。它揭示了大量的酚类化合物，其中绿单宁占检测到的化合物的50% %以上。通过估计每日摄入量（EDI）、目标危害商（THQ）和总癌症风险（TCR）分析确认了可接受的风险水平，即使考虑到无机砷的中等值。研究结果强调了这些大型藻类的营养价值和安全性，强调了它们在食品中的潜在应用。

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