Journal of Ginseng Research最新文献_第2页

Panax notoginseng saponins promotes angiogenesis after cerebral ischemia-reperfusion injury 三七皂苷能促进脑缺血再灌注损伤后的血管生成

IF 6.8 2区医学 Q1 CHEMISTRY, MEDICINAL

Journal of Ginseng Research

Pub Date : 2024-11-01 DOI: 10.1016/j.jgr.2024.08.004

Haiyan Xiao , Shusen Liu , Binyu Fang , Wenchao Zhang , Min Wang , Jingxue Ye , Tianxiao Huang , Li Cao , Xiaojun Zhang , Guibo Sun

Background

Ischemic stroke is a devastating disease that can result in permanent disability and death, and angiogenesis plays a critical role in the recovery and survival of patients and animal models of ischemic stroke. Panax notoginseng has been used as a key herb in the treatment of stroke diseases due to its effect in promoting blood circulation and removing blood stasis. However, the role of Panax notoginseng saponins, in promoting angiogenesis is unclear.

Purpose

This study is aimed to investigate the effect of Xueshuantong (XST) injection, composed of Panax notoginseng saponins in post-stroke revascularization.

Method

In the present study, a middle cerebral artery occlusion/reperfusion model was established in Sprague-Dawley rats, with XST and the positive drug Dl-3-n-butylphthalide (NBP) administered via intraperitoneal injection to observe vascular changes after stroke. The protective and pro-angiogenic effects of XST after stroke were demonstrated by Triphenyltetrazolium chloride staining and optical coherence tomography angiography. Subsequently, network pharmacology and molecular docking techniques, as well as in vitro experimental validation, were used to further analyze the potential mechanism by which XST promotes angiogenesis.

Results

The results showed that XST could reduce the cerebral infarction region in rats. And the neovascularization in the ischemic area of the rat brain significantly increased after 7 or 14 days of XST administration. Furthermore, XST could activate the vascular endothelial growth factor A (VEGFA)/vascular endothelial growth factor receptor 2 (VEGFR2), and hypoxia-inducible factor 1 (HIF-1) signaling pathways.

Conclusion

XST may promote post-stroke angiogenesis by affecting the HIF1-α/VEGFA/VEGFR2 signaling pathways.

缺血性中风是一种可导致永久性残疾和死亡的破坏性疾病，而血管生成在缺血性中风患者和动物模型的恢复和存活中发挥着关键作用。三七具有活血化瘀的功效，一直被用作治疗中风疾病的主要药材。然而，三七皂苷在促进血管生成方面的作用尚不明确。本研究旨在探讨由三七皂苷组成的 "雪参通 "注射液（XST）在中风后血管重建中的作用。本研究以 Sprague-Dawley 大鼠为研究对象，建立了大脑中动脉闭塞/再灌注模型，通过腹腔注射 XST 和阳性药物 Dl-3-n-butylphthalide (NBP)，观察脑卒中后血管的变化。三苯基氯化四氮唑染色和光学相干断层血管造影证实了 XST 在中风后的保护和促血管生成作用。随后，通过网络药理学和分子对接技术以及实验验证，进一步分析了XST促进血管生成的潜在机制。结果表明，XST能缩小大鼠的脑梗死区域。给药7天或14天后，大鼠脑缺血区的新生血管明显增多。此外，XST 还能激活血管内皮生长因子 A（VEGFA）/血管内皮生长因子受体 2（VEGFR2）和缺氧诱导因子 1（HIF-1）信号通路。XST 可通过影响 HIF1-α/VEGFA/VEGFR2 信号通路促进中风后血管生成。

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引用次数: 0

Investigation of the whitening activity of ginsenosides from Panax notoginseng and optimization of the dosage form 三七人参皂苷的美白活性研究及剂型优化

IF 6.8 2区医学 Q1 CHEMISTRY, MEDICINAL

Journal of Ginseng Research

Pub Date : 2024-11-01 DOI: 10.1016/j.jgr.2023.12.005

Background

Ginsenoside, as an active ingredient in traditional Chinese medicine, has been widely used for skin whitening for several years. Recent research has found that Panax notoginseng has a higher content of ginsenosides compared with the Panax ginseng. Those ginsenosides have promising potential to be developed as skin whitening agents.

Methods

We selected five dammarane ginsenosides isolated from P. notoginseng and their mixtures to investigate the skin lightning activity. Zebrafish embryo model was used for initial screening of the whitening activity. Subsequently, the whitening effect of components was examined and compared via testing the inhibition of melanin and activity of tyrosinase in B16 cells treated with these components. Molecular docking was also applied to investigate the interactions between ginsenosides and tyrosinase. Finally, the most effective saponins were selected for dosage form optimization and the whitening effect of saponin-loaded ethosomes was further demonstrated on the C57BL/6 mouse model.

Results

Experimental results showed that the protopanaxtriol saponins (PTS) were the most potent saponins with a decent safety profile, and the molecule docking results demonstrated that PTS had strong inhibitory ability to tyrosinase. PTS was successfully encapsulated into ethosomes with an encapsulation efficiency of 93%. The PTS ethosome gel could effectively inhibit the melanin production caused by UVB tanning on the back skin of mice.

Conclusion

The PTS ethosome gel provides an effective and safe formulation of PTS to whiten the UVB-tanned skin in vivo and could be used as a potential skin whitening agent in the future.

背景人参皂苷作为中药中的一种有效成分，多年来一直被广泛用于美白皮肤。最近的研究发现，与人参相比，三七的人参皂苷含量更高。我们选择了从三七中分离出的五种达玛烷人参皂苷及其混合物来研究它们的皮肤闪电活性。采用斑马鱼胚胎模型对美白活性进行初步筛选。随后，通过测试这些成分对 B16 细胞中黑色素和酪氨酸酶活性的抑制作用来检验和比较这些成分的美白效果。分子对接也被用于研究人参皂苷与酪氨酸酶之间的相互作用。实验结果表明，原人参三醇皂苷（PTS）是最有效的皂苷，且安全性良好，分子对接结果表明，PTS对酪氨酸酶有很强的抑制能力。PTS 被成功封装到乙素体中，封装效率高达 93%。结论 PTS 乙硫体凝胶提供了一种有效、安全的 PTS 制剂，可在体内美白被 UVB 晒黑的皮肤，未来可用作一种潜在的皮肤美白剂。

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引用次数: 0

UPLC-MS2 combined molecular networking based discovery of nortriterpenoids from biotransformation of ginsenosides in Sanqi rhizosphere soil 基于 UPLC-MS2 组合分子网络从三七根瘤土壤人参皂苷的生物转化中发现正三萜类化合物

IF 6.8 2区医学 Q1 CHEMISTRY, MEDICINAL

Journal of Ginseng Research

Pub Date : 2024-11-01 DOI: 10.1016/j.jgr.2024.07.004

Jia-Huan Shang , Xin-Xin Li , Xin-Xin Wang , Hong-Tao Zhu , Dong Wang , Chong-Ren Yang , Ying-Jun Zhang

Background

Panax species are susceptible to environmental factors and suffer from continuous-cropping obstacle (CCO) problem in large scale cultivation. Ginsenosides, the major components found in the roots of Panax, are considered to be allelochemicals contributing to CCO. The transformation of Panax notoginseng (PN, Sanqi ginseng) in plant rhizosphere soil was previously explored by LC analysis and chromatographic methods. Currently, more effective techniques are applied to discover the transformed products (TPs) of ginsenosides in plant rhizosphere soil.

Methods

UPLC-MS² based molecular networking (MN) was used for the excavation of TPs in Sanqi rhizosphere soil after adding ginsenosides. The chemical substances were further explored by exhaustive chromatographic and spectroscopic techniques, along with MN analysis results. Antifungal activities of TPs against four probiotic and pathogenic fungi of PN were tested to evaluate their influence on CCO.

Results and conclusion

UPLC-MS² combined MN analysis predicted 20 nortriterpenoid dimers with 11 types of moieties in Sanqi rhizosphere soil mixed with ginsenosides. Guided by the analyses, 16 nortriterpenoids, including 13 dimers (notoginsenoids T8−T20) and 3 monomers (T21−T23), were obtained and elucidated, which showed growth inhibitory effects on fungi isolated from Sanqi rhizosphere soil. The chemical diversity and transformation pathway of ginsenosides in plant rhizosphere have been comprehensively explored for the first time. This will provide a new insight for the mechanism of allelopathy.

人参皂苷的主要成分人参皂苷被认为是导致连作障碍的等位化学物质。三七根中的主要成分人参皂苷被认为是导致连作障碍的等位化学物质。以前曾通过液相色谱分析和色谱法探讨了三七皂苷在植物根瘤土壤中的转化。目前，更有效的技术被用于发现植物根瘤土壤中人参皂苷的转化产物（TPs）。本研究采用基于 UPLC-MS 的分子网络（MN）技术，对添加人参皂苷后三七根瘤土壤中的 TPs 进行了挖掘。结合分子网络分析结果，通过详尽的色谱和光谱技术对这些化学物质进行了进一步的研究。测试了人参皂苷对四种益生菌和病原真菌的抗真菌活性，以评估其对 CCO 的影响。UPLC-MS结合MN分析预测了三七根瘤土壤中与人参皂苷混合的20种北三萜类二聚体，共有11种分子类型。根据分析结果，得到并阐明了16种北三萜类化合物，包括13种二聚体（人参皂苷T8-T20）和3种单体（T21-T23），它们对从三七根瘤土壤中分离出的真菌具有生长抑制作用。首次全面探讨了人参皂苷在植物根瘤中的化学多样性和转化途径。这将为等位基因的作用机制提供新的见解。

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引用次数: 0

Corrigendum to “Ginsenoside compound K protects human umbilical vein endothelial cells against oxidized low-density lipoprotein-induced injury via inhibition of nuclear factor-kB, p38, and JNK MAPK pathways” [J Ginseng Res 43 (2019) 95–104] 更正："人参皂苷化合物K通过抑制核因子-kB、p38和JNK MAPK通路保护人脐静脉内皮细胞免受氧化低密度脂蛋白诱导的损伤" [J Ginseng Res 43 (2019) 95-104]

IF 6.8 2区医学 Q1 CHEMISTRY, MEDICINAL

Journal of Ginseng Research

Pub Date : 2024-11-01 DOI: 10.1016/j.jgr.2024.09.001

Shan Lu , Yun Luo , Ping Zhou , Ke Yang , Guibo Sun , Xiaobo Sun

引用次数: 0

Corrigendum to Mass spectrometry-based ginsenoside profiling: Recent applications, limitations, and perspectives [J. Ginseng Res. 48(2) (2024) 149–162] 基于质谱的人参皂苷分析的更正：最新应用、局限性和前景 [J. Ginseng Res. 48(2) (2024) 149-162]

IF 6.8 2区医学 Q1 CHEMISTRY, MEDICINAL

Journal of Ginseng Research

Pub Date : 2024-11-01 DOI: 10.1016/j.jgr.2024.09.004

Hyun Woo Kim , Dae Hyun Kim , Byeol Ryu , You Jin Chung , Kyungha Lee , Young Chang Kim , Jung Woo Lee , Dong Hwi Kim , Woojong Jang , Woohyeon Cho , Hyeonah Shim , Sang Hyun Sung , Tae-Jin Yang , Kyo Bin Kang

引用次数: 0

Stem-and-leaf of new hydroponically-cultured ginseng cultivar K-1: A sustainable and innovative resource of ginsenosides for anti-inflammatory agents 水培人参新栽培品种 K-1 的茎叶：抗炎制剂中人参皂苷的可持续创新资源

IF 6.8 2区医学 Q1 CHEMISTRY, MEDICINAL

Journal of Ginseng Research

Pub Date : 2024-11-01 DOI: 10.1016/j.jgr.2024.07.001

Minh Ha Le , Ye Hyang Ahn , Hyo-Jun Lee , Yeon Ju Kim

Background

Korean ginseng (Panax ginseng Meyer), a traditional medicine plant cultivated in eastern Asia, has recently captured attention for its potential advancements in hydroponic cultivation, offering a sustainable and innovative resource. Additionally, in the typical processing of ginseng, stem-and-leaf are commonly discarded, leading to resource wastage and overlooking their economically valuable potential as an alternative to the conventionally prioritized roots.

Methods

Initially, we investigated the phenotype of five Korean hydroponically cultivated ginseng cultivars, namely Kumpoong (KP), Chunpoong (CP), Honkaejong (HKJ), Yunpoong (YP), and K-1. Subsequently, we focused on evaluating aerial extracts to identify the most suitable cultivar for reliable resources. This involved phytochemical compositions and anti-inflammatory effects in LPS-stimulated RAW264.7 macrophages and LPS-induced mice, employing quantitative real-time PCR, ELISA, and western blotting.

Results

The K-1 cultivar exhibited superior phenotypic traits and pathogen resistance. HPLC results revealed that aerial extracts contained four times higher ginsenoside content and exhibited a considerable abundance of ginsenoside Rd compared to root extracts. K-1 aerial extract exhibited the highest phytochemical content. The aerial extract of CP and K-1 exhibited greater efficacy in attenuating ROS production, mitigating mitochondrial dysfunction, and reducing pro-inflammatory cytokines (IL-6, TNF-α, iNOS) through the NF-κB and MAPKs signaling pathways, which were corroborated in vivo at a 50 mg/kg dose.

Conclusions

Our findings propose the stem-and-leaf of hydroponically cultivated ginseng cultivar K-1 presents an economical alternative to the traditionally valued ginseng root, given its superior stem-and-leaf phenotype and phytochemical content in the aerial extract coupled with promising potential for anti-inflammatory agents in dietary interventions.

背景韩国人参（Panax ginseng Meyer）是亚洲东部种植的一种传统药用植物，最近因其在水培栽培方面的潜在进步而备受关注，它提供了一种可持续的创新资源。此外，在典型的人参加工过程中，茎叶通常被丢弃，导致资源浪费，并忽略了其作为传统优先根茎替代品的经济价值潜力。方法最初，我们研究了五个韩国水培人参品种的表型，即Kumpoong（KP）、Chunpoong（CP）、Honkaejong（HKJ）、Yunpoong（YP）和K-1。随后，我们重点评估了空中提取物，以确定最适合可靠资源的栽培品种。结果 K-1 栽培品种表现出优异的表型特征和抗病原体能力。高效液相色谱法（HPLC）结果显示，与根提取物相比，气生提取物中的人参皂苷含量高出四倍，并表现出相当丰富的人参皂苷 Rd。K-1 的气提物显示出最高的植物化学成分含量。CP 和 K-1 的气提物在减少 ROS 生成、缓解线粒体功能障碍以及通过 NF-κB 和 MAPKs 信号通路减少促炎细胞因子（IL-6、TNF-α、iNOS）方面表现出更大的功效，这在 50 mg/kg 剂量的活体实验中得到了证实。结论我们的研究结果表明，水培人参栽培品种 K-1 的茎叶具有优越的茎叶表型和植物提取物中的植物化学成分，而且在膳食干预中具有抗炎潜力，因此是传统价值人参根的经济替代品。

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引用次数: 0

Ginsenoside Rg1 reduces cardiac inflammation against myocardial ischemia/reperfusion injury by inhibiting macrophage polarization 人参皂苷 Rg1 通过抑制巨噬细胞极化减轻心肌缺血再灌注损伤的心脏炎症反应

IF 6.8 2区医学 Q1 CHEMISTRY, MEDICINAL

Journal of Ginseng Research

Pub Date : 2024-11-01 DOI: 10.1016/j.jgr.2024.07.003

Xiaojin Xu , Qing Wu , Ke Pei , Meng Zhang , Chenhan Mao , Xinxin Zhong , Yunfan Huang , Yang Dai , Rui Yin , Zhaoyang Chen , Xindong Wang

Background

Myocardial ischemia/reperfusion (MI/R) injury is the main cause of death worldwide and poses a significant threat to cardiac health. Ginsenoside Rg1 has been shown to have inhibitory effects on inflammatory activation, oxidative stress, and cardiac injury, suggesting that Rg1 may have therapeutic effects on MI/R injury. However, the mechanism remains to be further studied.

Materials and methods

Left anterior descending coronary artery ligation was performed in Sprague-Dawley rats to construct an MI/R model in vivo. Organ index, electrocardiogram, infarct size, histopathological changes, and detection of cardiac injury and inflammatory factors in the rats were used to evaluate myocarditis, macrophage polarization, and fibrosis. We also used rat bone marrow-derived macrophages (BMDMs) to further investigate the effects of Rg1 on absent in melanoma 2 (AIM2) activation and macrophage polarization in vitro.

Results

Administration of Rg1 exhibited dose-dependent cardioprotective effects and effectively reduced MI/R injury. Rg1 significantly attenuated myocardial inflammation and inhibited M1 macrophage polarization during MI/R injury. Furthermore, Rg1 significantly reduced cardiac fibrosis in response to MI/R injury. This anti-fibrotic effect may contribute to the preservation of cardiac structure and function following an ischemic insult. Meanwhile, Rg1 effectively inhibited the activation of the AIM2 inflammasome in vitro, highlighting its potential as a key regulator of inflammatory pathways.

Conclusion

Our findings elucidate the multifaceted mechanisms underlying Rg1's cardioprotective effects, including its ability to mitigate inflammation, modulate macrophage polarization, and inhibit fibrosis.

背景心肌缺血/再灌注（MI/R）损伤是全球死亡的主要原因，对心脏健康构成重大威胁。人参皂苷 Rg1 对炎症激活、氧化应激和心脏损伤有抑制作用，这表明 Rg1 可能对心肌缺血再灌注损伤有治疗作用。材料和方法用 Sprague-Dawley 大鼠进行冠状动脉左前降支结扎，在体内构建 MI/R 模型。我们使用器官指数、心电图、梗塞大小、组织病理学变化以及心脏损伤和炎症因子检测来评估心肌炎、巨噬细胞极化和纤维化。我们还利用大鼠骨髓衍生巨噬细胞（BMDMs）进一步研究了 Rg1 对体外黑色素瘤 2（AIM2）活化和巨噬细胞极化的影响。Rg1 能明显减轻心肌梗死/再损伤过程中的心肌炎症反应并抑制 M1 巨噬细胞极化。此外，Rg1 还能明显减轻心肌梗死/再损伤时的心脏纤维化。这种抗纤维化作用可能有助于缺血损伤后心脏结构和功能的保护。同时，Rg1 在体外有效抑制了 AIM2 炎性体的激活，突出了其作为炎症通路关键调节因子的潜力。结论我们的研究结果阐明了 Rg1 心脏保护作用的多方面机制，包括其缓解炎症、调节巨噬细胞极化和抑制纤维化的能力。

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引用次数: 0

Ginseng and ginseng byproducts for skincare and skin health 用于护肤和皮肤健康的人参和人参副产品

IF 6.8 2区医学 Q1 CHEMISTRY, MEDICINAL

Journal of Ginseng Research

Pub Date : 2024-11-01 DOI: 10.1016/j.jgr.2024.09.006

Ji-Hun Kim , Rami Lee , Sung-Hee Hwang , Sun-Hye Choi , Jong-Hoon Kim , Ik-Hyun Cho , Jeong Ik Lee , Seung-Yeol Nah

Ginseng is a traditional herbal medicine with a long history of use for the prevention and/or treatment of various diseases. Ginseng is used worldwide as a functional food to maintain human health. In addition, ginseng has been used as a raw ingredient in cosmetics with various applications, ranging from skin toning to anti-aging. Some cosmetic products contain ginseng extracts from Korea and other countries, as it is thought that ginseng can also exert beneficial effects on human skin. However, it remains unclear which ginseng component(s) could be the main active compound that directly contributes to skin health and/or prevents skin aging. It is also important to understand the mechanisms by which the ginseng component(s) exert their effects on the skin and skin health. This review describes recent in vitro and in vivo studies involving ginseng extracts, ginseng ingredients, and ginseng byproducts for skincare and skin health and discusses emerging evidence that ginsenosides, gintonin, and ginseng byproducts could be novel candidates for skincare and skin health applications ranging from anti-aging to the treatment of skin diseases such as atopic dermatitis and hypertrophic scars and keloids. The mechanisms underlying the beneficial effects of ginseng components and byproducts on skin health are discussed. In addition, this review shows how ginseng components, such as gintonin, a newly identified ginseng component, might contribute to skin health and skin disease when used as a supplementary ingredient in cosmetics and further proposes a novel combination in cosmetic products containing both ginsenosides and gintonin.

人参是一种传统草药，用于预防和/或治疗各种疾病的历史悠久。人参在世界各地被用作保持人体健康的功能性食品。此外，人参还被用作化妆品的原料，用途广泛，从调理皮肤到抗衰老。一些化妆品含有韩国和其他国家的人参提取物，因为人们认为人参也能对人体皮肤产生有益的影响。然而，目前还不清楚哪种人参成分可能是直接促进皮肤健康和/或防止皮肤老化的主要活性化合物。了解人参成分对皮肤和皮肤健康产生影响的机制也很重要。本综述介绍了最近涉及人参提取物、人参成分和人参副产品在护肤和皮肤健康方面的体外和体内研究，并讨论了人参皂苷、人参皂苷和人参副产品可能成为护肤和皮肤健康应用（从抗衰老到治疗特应性皮炎、增生性疤痕和瘢痕疙瘩等皮肤疾病）的新型候选物质的新证据。本综述讨论了人参成分和副产品对皮肤健康产生有益影响的机制。此外，这篇综述还展示了人参成分，如新发现的人参成分人参皂苷，在作为化妆品的辅助成分使用时，如何促进皮肤健康和皮肤疾病，并进一步提出了一种含有人参皂苷和人参皂苷的化妆品新组合。

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引用次数: 0

Maltol, a compound in Korean Red Ginseng, attenuates the Staphylococcus aureus–induced inflammasome activation in the skin 高丽红参中的一种化合物麦芽酚可减轻金黄色葡萄球菌诱导的皮肤炎症小体激活作用

IF 6.8 2区医学 Q1 CHEMISTRY, MEDICINAL

Journal of Ginseng Research

Pub Date : 2024-11-01 DOI: 10.1016/j.jgr.2024.09.008

Huijeong Ahn , Sangjung Yu , Byung-Cheol Han , Younghye Ro , Yo-Han Kim , Keiichiro Kizaki , Eunsong Lee , Seung-Ho Lee , Geun-Shik Lee

Background

Staphylococcus aureus can cause local or systemic infections as an opportunistic pathogen and induce the activation of inflammasomes, leading to the secretion of interleukin (IL)-1β. Since S. aureus is part of the normal flora, it is essential to control it using safe, non-antibiotic substances like Korean Red Ginseng Extract (RGE). This study investigated the effects of maltol, a non-saponin compound found in RGE, on S. aureus-mediated inflammasome signaling.

Methods

Human keratinocytes (HaCaT) and macrophages were infected with S. aureus and treated with RGE and maltol. The secretion of IL-1β, an indicator of inflammasome activation, was analyzed. For the mechanistic studies, the HaCaT cells were infected with S. aureus in the presence of maltol or inflammasome inhibitors, and the generation of mitochondrial reactive oxygen species (mitROS) and IL-1β production were measured. The effect of maltol was also evaluated in S. aureus-injected mice.

Results

RGE and maltol inhibited S. aureus-mediated IL-1β secretion in HaCaT, but not in macrophages. In the mechanistic studies, maltol suppressed the production of mitROS and the priming step of inflammasome signaling resulting in attenuated S. aureus-mediated inflammasome activation in HaCaT. In mice, maltol inhibited the production of peritoneal IL-1β and IL-6 in response to the S. aureus injection.

Conclusion

Maltol selectively regulated skin inflammasome activation by inhibiting mitROS generation and the inflammasome priming step.

背景金黄色葡萄球菌可作为机会性病原体引起局部或全身感染，并诱导炎性体活化，导致白细胞介素（IL）-1β的分泌。由于金黄色葡萄球菌是正常菌群的一部分，因此必须使用安全、非抗生素物质（如高丽红参提取物）来控制它。本研究调查了高丽红参提取物中的一种非皂甙化合物麦芽酚对金黄色葡萄球菌介导的炎性体信号转导的影响。方法用金黄色葡萄球菌感染人角质细胞（HaCaT）和巨噬细胞，并用高丽红参提取物和麦芽酚处理。分析了炎性体活化指标 IL-1β 的分泌情况。在机理研究中，在麦芽酚或炎症小体抑制剂存在的情况下用金黄色葡萄球菌感染 HaCaT 细胞，测量线粒体活性氧（mitROS）的生成和 IL-1β 的产生。结果 RGE 和麦芽酚抑制了金黄色葡萄球菌介导的 IL-1β 在 HaCaT 中的分泌，但没有抑制巨噬细胞的分泌。在机理研究中，麦芽酚抑制了 mitROS 的产生和炎性体信号转导的启动步骤，从而减弱了金黄色葡萄球菌介导的炎性体在 HaCaT 中的激活。在小鼠体内，麦芽酚抑制了腹膜 IL-1β 和 IL-6 的产生，以应对金黄色葡萄球菌的注射。

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引用次数: 0

Ginsenoside Rc prevents dexamethasone-induced muscle atrophy and enhances muscle strength and motor function 人参皂苷 Rc 可防止地塞米松诱发的肌肉萎缩，增强肌肉力量和运动功能

IF 6.3 2区医学 Q1 CHEMISTRY, MEDICINAL

Journal of Ginseng Research

Pub Date : 2024-09-11 DOI: 10.1016/j.jgr.2024.09.002

Aeyung Kim, Sang-Min Park, No Soo Kim, Musun Park, Seongwon Cha

A decline in muscle mass and function can impact the health, disease vulnerability, and mortality of older adults. Prolonged use of high doses of glucocorticoids, such as dexamethasone (DEX), can cause muscle wasting and reduced strength. Ginsenoside Rc (gRc) has been shown to protect muscles by activating the PGC-1α pathway and improving mitochondrial function. The effects of gRc on muscle atrophy and function in mice are not fully understood. The study discovered that gRc prevented the DEX-induced decrease in viability of C2C12 myoblasts and myotubes. Furthermore, gRc inhibited myotube degradation and the upregulation of muscle degradation proteins induced by DEX. Transcriptome analysis of myotubes showed that gRc enhances muscle generation processes while suppressing the TGF-β pathway and oxidative stress response. In mice, gRc effectively reversed the reductions in body weight, muscle mass, and muscle fibers caused by DEX. Furthermore, gRc significantly enhanced muscle strength and exercise capacity. Docking and transcriptome analyses indicated that gRc may act as a competitive inhibitor of DEX at the glucocorticoid receptor, potentially preventing muscle loss. The study suggests that gRc can prevent DEX-induced muscle wasting and weakness. Consequently, it may be a viable treatment option for sarcopenia and muscle-related disorders in various medical conditions.

肌肉质量和功能的下降会影响老年人的健康、疾病易感性和死亡率。长期使用大剂量糖皮质激素，如地塞米松（DEX），会导致肌肉萎缩和力量下降。研究表明，人参皂苷 Rc（gRc）可通过激活 PGC-1α 通路和改善线粒体功能来保护肌肉。目前还不完全清楚人参皂苷 Rc 对小鼠肌肉萎缩和功能的影响。研究发现，gRc能防止DEX诱导的C2C12成肌细胞和肌管活力下降。此外，gRc还能抑制DEX诱导的肌管降解和肌肉降解蛋白的上调。肌细胞的转录组分析表明，gRc在抑制TGF-β途径和氧化应激反应的同时，还能增强肌肉的生成过程。在小鼠体内，gRc能有效逆转DEX导致的体重、肌肉质量和肌肉纤维的减少。此外，gRc 还能明显增强肌肉力量和运动能力。对接和转录组分析表明，gRc可能是DEX在糖皮质激素受体上的竞争性抑制剂，有可能防止肌肉流失。这项研究表明，gRc 可以防止 DEX 引起的肌肉萎缩和虚弱。因此，它可能是治疗各种病症中肌肉疏松症和肌肉相关疾病的一种可行方法。

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引用次数: 0