Pub Date : 2016-01-01DOI: 10.5455/jeim.270816.or.157
T. Igonina, T. Alekhina, N. Palchikova, O. Prokudina
Objective: The study was aimed at determining deviations in locomotor behavior and in levels of its regulators (serotonin, noradrenaline, and corticosterone) at the critical periods in rats of the genetic catatonia (GC) strain. Methods: The rat pups of GC and Wistar strains of different ages ranging from 1 to 14 days were tested by motor subsystem test. The following parameters were accounted: head movements, body movements, locomotion (pacing), immobility time, asymmetric postures during immobility periods, falls onto the back, retropulsions, body tremor, and swimming-like movements. Brain monoamine levels and corticosterone in blood plasma were assayed. The body and organ weights were measurement. Results: Preceding signs of catatonia have been noted in postnatal development in GC pups: (a) the prevalence of dyskinetic movements in the first days; (b) reduction in motor activity; (c) imbalance between the brain serotonin and noradrenaline levels; (d) increased plasma corticosterone concentration; (e) underweight at 1, 7, 10 and 14 days during early development, and the relatively larger weight of the brains and kidneys in GC rats compared with Wistar control. Conclusion: The entire range of observed abnormalities in the behavioral, neurotransmitter, hormonal, and general physical systems may be viewed as prodromal signs of catatonic reactions in GC rats.
{"title":"Prodromal signs of catatonia are associated with hereditary dysfunction of body systems in rat pups","authors":"T. Igonina, T. Alekhina, N. Palchikova, O. Prokudina","doi":"10.5455/jeim.270816.or.157","DOIUrl":"https://doi.org/10.5455/jeim.270816.or.157","url":null,"abstract":"Objective: The study was aimed at determining deviations in locomotor behavior and in levels of its regulators (serotonin, noradrenaline, and corticosterone) at the critical periods in rats of the genetic catatonia (GC) strain. Methods: The rat pups of GC and Wistar strains of different ages ranging from 1 to 14 days were tested by motor subsystem test. The following parameters were accounted: head movements, body movements, locomotion (pacing), immobility time, asymmetric postures during immobility periods, falls onto the back, retropulsions, body tremor, and swimming-like movements. Brain monoamine levels and corticosterone in blood plasma were assayed. The body and organ weights were measurement. Results: Preceding signs of catatonia have been noted in postnatal development in GC pups: (a) the prevalence of dyskinetic movements in the first days; (b) reduction in motor activity; (c) imbalance between the brain serotonin and noradrenaline levels; (d) increased plasma corticosterone concentration; (e) underweight at 1, 7, 10 and 14 days during early development, and the relatively larger weight of the brains and kidneys in GC rats compared with Wistar control. Conclusion: The entire range of observed abnormalities in the behavioral, neurotransmitter, hormonal, and general physical systems may be viewed as prodromal signs of catatonic reactions in GC rats.","PeriodicalId":16091,"journal":{"name":"Journal of Experimental and Integrative Medicine","volume":"22 1","pages":"99-108"},"PeriodicalIF":0.0,"publicationDate":"2016-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83340235","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2015-12-01DOI: 10.5455/JEIM.231115.OR.140
Mohamed F Balaha, Samah Kandeel, Hiroyuki Tanaka, H. Yamashita, M. Abdel-Rahman, N. Inagaki
Objectives: Lovastatin (LOV) is a cholesterol-lowering agent with immunomodulatory and anti-inflammatory effects. The present study evaluated the immunomodulatory effects of LOV in a mouse model of bronchial asthma. Methods: Mice were sensitized by giving 50 μg ovalbumin (OVA) i.p. with 1 mg alum on days 0 and 12. From day 22, mice were exposed to OVA (1% (w/v) in saline for 30 min, three times every 4th day. Negative control received saline similarly. Oral LOV, given 31 days, was starting from day 0 to day 30 and at sensitization day; it was given 30 min before the treatment. The number of inflammatory cells, levels of interleukin (IL)-4, IL-5, IL-13 and interferon (IFN)- in bronchoalveolar lavage fluid (BALF), serum IgE, OVA-specific IgE, IgG1 and IgG2a levels, in addition to histopathological and immunohistochemical examination of the lung were investigated. Results: LOV showed significant decrease in the number of leukocytes, macrophages and eosinophils, levels of IL-4, IL-5 and IL-13 in BALF, serum levels of IgE, OVA-specific IgE and IgG1, but no significant effect on BALF level of IFN- and serum level of OVA-specific IgG2a, in addition to the improvement of the histopathological and immunohistochemical changes. Conclusion: These results suggest that LOV could be beneficial for the treatment of bronchial asthma.
{"title":"Immunomodulatory effects of lovastatin on ovalbumin-induced bronchial asthma in mice","authors":"Mohamed F Balaha, Samah Kandeel, Hiroyuki Tanaka, H. Yamashita, M. Abdel-Rahman, N. Inagaki","doi":"10.5455/JEIM.231115.OR.140","DOIUrl":"https://doi.org/10.5455/JEIM.231115.OR.140","url":null,"abstract":"Objectives: Lovastatin (LOV) is a cholesterol-lowering agent with immunomodulatory and anti-inflammatory effects. The present study evaluated the immunomodulatory effects of LOV in a mouse model of bronchial asthma. \u0000Methods: Mice were sensitized by giving 50 μg ovalbumin (OVA) i.p. with 1 mg alum on days 0 and 12. From day 22, mice were exposed to OVA (1% (w/v) in saline for 30 min, three times every 4th day. Negative control received saline similarly. Oral LOV, given 31 days, was starting from day 0 to day 30 and at sensitization day; it was given 30 min before the treatment. The number of inflammatory cells, levels of interleukin (IL)-4, IL-5, IL-13 and interferon (IFN)- in bronchoalveolar lavage fluid (BALF), serum IgE, OVA-specific IgE, IgG1 and IgG2a levels, in addition to histopathological and immunohistochemical examination of the lung were investigated. \u0000Results: LOV showed significant decrease in the number of leukocytes, macrophages and eosinophils, levels of IL-4, IL-5 and IL-13 in BALF, serum levels of IgE, OVA-specific IgE and IgG1, but no significant effect on BALF level of IFN- and serum level of OVA-specific IgG2a, in addition to the improvement of the histopathological and immunohistochemical changes. \u0000Conclusion: These results suggest that LOV could be beneficial for the treatment of bronchial asthma.","PeriodicalId":16091,"journal":{"name":"Journal of Experimental and Integrative Medicine","volume":"167 1","pages":"183-192"},"PeriodicalIF":0.0,"publicationDate":"2015-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74929986","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2015-12-01DOI: 10.5455/JEIM.051115.OR.137
L. F. Otoo, C. Ansah, G. Koffuor, C. Benneh, K. Mensah, I. Ben
Objective: Picralima nitida is widely used in the traditional management of several ailments. This study therefore aimed to investigate the effect of an ethanolic seed extract of P.nitida on reproductive abilities and developmental indices and to establish its safety for use. Methods: Secondary metabolite screening, and safety assessment to estimate the NOAEL, and LD50 of P.nitida were conducted. Mating, estrous, fertility, live birth, and weaning indices, and the gestation period in female rodents pretreated with P.nitida (10-1000 mg/kg) for 14 days were determined. In males the number of attempted mounts, sniffing, licking, genital and non-genital grooming, and hesitation time to female, in reproductive orientation (mating) behavior studies were determined with P.nitida treatments. Spermatocyte count and quality (morphology, motility and viability) as well as histopathological study of the seminiferous tubules were also performed. Results: P.nitida, which had alkaloids, tannins, steroids, glycosides, anthraquinones and terpenoids, present a NOAEL 2000 mg/kg. Treatment with P.nitida in females significantly reduced fertility index (40%), caused dose-dependent reduction in litter size and increased estrous index. Mating index, gestation period, live birth index and weaning index were not affected. In males, there was a significant increase P.nitida-induced sniffing, licking, genital and non-genital grooming and attempted mounts with acute (but not prolonged) treatment. Treatment also significantly reduced the hesitation time of males for attraction towards females and sperm count. Spermatocyte morphology, motility and viability was not affected. Histopathological studies indicated progressive degenerative changes in the seminiferous tubules with increasing P.nitida doses. Conclusion: Picralima nitida enhances sexual behaviors in both males and females. However, chronic administration reduces fertility in females and sperm count in males; at relatively lower doses the extract is safe to use.
{"title":"Effect of an ethanolic seed extract of Picralima nitida ((Stapf) Th. & H. Durand) on reproductive abilities and developmental indices in rodents -","authors":"L. F. Otoo, C. Ansah, G. Koffuor, C. Benneh, K. Mensah, I. Ben","doi":"10.5455/JEIM.051115.OR.137","DOIUrl":"https://doi.org/10.5455/JEIM.051115.OR.137","url":null,"abstract":"Objective: Picralima nitida is widely used in the traditional management of several ailments. This study therefore aimed to investigate the effect of an ethanolic seed extract of P.nitida on reproductive abilities and developmental indices and to establish its safety for use. \u0000Methods: Secondary metabolite screening, and safety assessment to estimate the NOAEL, and LD50 of P.nitida were conducted. Mating, estrous, fertility, live birth, and weaning indices, and the gestation period in female rodents pretreated with P.nitida (10-1000 mg/kg) for 14 days were determined. In males the number of attempted mounts, sniffing, licking, genital and non-genital grooming, and hesitation time to female, in reproductive orientation (mating) behavior studies were determined with P.nitida treatments. Spermatocyte count and quality (morphology, motility and viability) as well as histopathological study of the seminiferous tubules were also performed. \u0000Results: P.nitida, which had alkaloids, tannins, steroids, glycosides, anthraquinones and terpenoids, present a NOAEL 2000 mg/kg. Treatment with P.nitida in females significantly reduced fertility index (40%), caused dose-dependent reduction in litter size and increased estrous index. Mating index, gestation period, live birth index and weaning index were not affected. In males, there was a significant increase P.nitida-induced sniffing, licking, genital and non-genital grooming and attempted mounts with acute (but not prolonged) treatment. Treatment also significantly reduced the hesitation time of males for attraction towards females and sperm count. Spermatocyte morphology, motility and viability was not affected. Histopathological studies indicated progressive degenerative changes in the seminiferous tubules with increasing P.nitida doses. \u0000Conclusion: Picralima nitida enhances sexual behaviors in both males and females. However, chronic administration reduces fertility in females and sperm count in males; at relatively lower doses the extract is safe to use.","PeriodicalId":16091,"journal":{"name":"Journal of Experimental and Integrative Medicine","volume":"23 1","pages":"200-208"},"PeriodicalIF":0.0,"publicationDate":"2015-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87537995","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2015-01-01DOI: 10.5455/JEIM.231115.OR.141
O. Nardid, S. Repina, E. Rozanova, Y. Cherkashina, E. O. Nardid
Objective: In this study we analyzed the influence of low temperature storage of placental tissue on the effect of obtained aqueous-saline placental extracts and their extract fractions on the state of membranes and cytosol of erythrocytes. Methods: Fractions of human placenta extract (HPE) were obtained with gel-chromatography method. Spontaneous hemolysis of erythrocytes was measured spectrophotometrically by free hemoglobin content in supernatant at 543 nm wavelength. Osmotic fragility (resistance) of erythrocytes was assessed by the level of hemolysis in 0.45% NaCl solution. Low-pH resistance of erythrocytes was evaluated by the time of 50% hemolysis in sodium-phosphate-citrate buffer (pH 3.8), calculating the data from kinetic curve of the change of optical density at 700 nm. Electron paramagnetic resonance (EPR) spin probe method was applied for analysis of cytosol dynamic state. Results: We recorded that low temperature storage of placental tissue allows decreasing membranotropic effect, which was inherent to certain aqueous-saline placental extracts and some of their fractions. At the same time, storage of placental tissue at both 20 С and 196 С does not influence the ability of HPE to decrease erythrocyte osmotic fragility. Erythrocytes exposed with HPE derived from placenta stored for 6 months at 20 С displayed decrease of low-pH resistance. This effect was inherent not only to the whole placental extract, but also to its certain fractions, especially those with molecular weight below 5 kDa. Conclusions: Storage of placental tissue at 196 С seems to be advantageous for preservation of studied properties of placental aqueous-saline extract in comparison to storage at 20 С.
{"title":"Properties of aqueous-saline human placental extracts and their fractions after storage of placenta at various subzero temperatures -","authors":"O. Nardid, S. Repina, E. Rozanova, Y. Cherkashina, E. O. Nardid","doi":"10.5455/JEIM.231115.OR.141","DOIUrl":"https://doi.org/10.5455/JEIM.231115.OR.141","url":null,"abstract":"Objective: In this study we analyzed the influence of low temperature storage of placental tissue on the effect of obtained aqueous-saline placental extracts and their extract fractions on the state of membranes and cytosol of erythrocytes. Methods: Fractions of human placenta extract (HPE) were obtained with gel-chromatography method. Spontaneous hemolysis of erythrocytes was measured spectrophotometrically by free hemoglobin content in supernatant at 543 nm wavelength. Osmotic fragility (resistance) of erythrocytes was assessed by the level of hemolysis in 0.45% NaCl solution. Low-pH resistance of erythrocytes was evaluated by the time of 50% hemolysis in sodium-phosphate-citrate buffer (pH 3.8), calculating the data from kinetic curve of the change of optical density at 700 nm. Electron paramagnetic resonance (EPR) spin probe method was applied for analysis of cytosol dynamic state. Results: We recorded that low temperature storage of placental tissue allows decreasing membranotropic effect, which was inherent to certain aqueous-saline placental extracts and some of their fractions. At the same time, storage of placental tissue at both 20 С and 196 С does not influence the ability of HPE to decrease erythrocyte osmotic fragility. Erythrocytes exposed with HPE derived from placenta stored for 6 months at 20 С displayed decrease of low-pH resistance. This effect was inherent not only to the whole placental extract, but also to its certain fractions, especially those with molecular weight below 5 kDa. Conclusions: Storage of placental tissue at 196 С seems to be advantageous for preservation of studied properties of placental aqueous-saline extract in comparison to storage at 20 С.","PeriodicalId":16091,"journal":{"name":"Journal of Experimental and Integrative Medicine","volume":"33 1","pages":"172-177"},"PeriodicalIF":0.0,"publicationDate":"2015-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77595546","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2015-01-01DOI: 10.5455/JEIM.230115.OR.121
T. Khan, R. Raina, P. Verma, M. Sultana, Ahmad Mahrukh
Objective: Imbalance between the concentrations of oxidant and antioxidants in mammalian cells leads to oxidative stress. The present study aimed to evaluate the protective effect of Ipomoea carnea Jacq leaves extracts on streptozotocin (STZ)-induced oxidative stress in rats. Methods: Alterations in stress biomarkers like activities of superoxide dismutase, catalase, glutathione peroxidase, and levels of blood glutathione and malondialdehyde in experimental groups administered with STZ alone and along with aqueous or alcoholic extracts of I.carnea were determined. Results: Single intra-peritoneal administration of STZ (60 mg/kg) caused significant decrease in activities of superoxide dismutase, catalase, glutathione peroxidase, and blood glutathione levels while malondialdehyde level as an indicative of oxidative stress significant increased. Administration of I.carnea extracts at the dose rate of 500 mg/kg body weight through oral gavage continuously for 3 weeks in STZ-treated group reduced malondialdehyde level significantly and increased activities of superoxide dismutase, catalase, glutathione peroxidase, and blood glutathione level as compared to STZ alone treated rats; however, the values of antioxidants remain still lower in comparison to normal control rats. Conclusion: In the present STZ-diabetes model of rats, alcoholic extract of I.carnea leaves have more potent efficacy in restoring the enzymatic and non-enzymatic components of antioxidant system of rats as than the aqueous extract.
{"title":"Protective effect of Ipomoea carnea Jacq leaves extracts on streptozotocin-induced oxidative stress in rats -","authors":"T. Khan, R. Raina, P. Verma, M. Sultana, Ahmad Mahrukh","doi":"10.5455/JEIM.230115.OR.121","DOIUrl":"https://doi.org/10.5455/JEIM.230115.OR.121","url":null,"abstract":"Objective: Imbalance between the concentrations of oxidant and antioxidants in mammalian cells leads to oxidative stress. The present study aimed to evaluate the protective effect of Ipomoea carnea Jacq leaves extracts on streptozotocin (STZ)-induced oxidative stress in rats. Methods: Alterations in stress biomarkers like activities of superoxide dismutase, catalase, glutathione peroxidase, and levels of blood glutathione and malondialdehyde in experimental groups administered with STZ alone and along with aqueous or alcoholic extracts of I.carnea were determined. Results: Single intra-peritoneal administration of STZ (60 mg/kg) caused significant decrease in activities of superoxide dismutase, catalase, glutathione peroxidase, and blood glutathione levels while malondialdehyde level as an indicative of oxidative stress significant increased. Administration of I.carnea extracts at the dose rate of 500 mg/kg body weight through oral gavage continuously for 3 weeks in STZ-treated group reduced malondialdehyde level significantly and increased activities of superoxide dismutase, catalase, glutathione peroxidase, and blood glutathione level as compared to STZ alone treated rats; however, the values of antioxidants remain still lower in comparison to normal control rats. Conclusion: In the present STZ-diabetes model of rats, alcoholic extract of I.carnea leaves have more potent efficacy in restoring the enzymatic and non-enzymatic components of antioxidant system of rats as than the aqueous extract.","PeriodicalId":16091,"journal":{"name":"Journal of Experimental and Integrative Medicine","volume":"2 1","pages":"42-46"},"PeriodicalIF":0.0,"publicationDate":"2015-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87545001","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2015-01-01DOI: 10.5455/JEIM.300315.ED.019
S. Oter, K. Şimşek
With the beginning of 2011, the “Journal of Experimental and Integrative Medicine” launched the medical literature with the motto “from cell to system, from mechanism to disease” [1]. The scope of the journal covered the entire field of biomedical research with particular interest on integrating experimental data to clinical applications; in other words, the so-called “bench-to-bedside” or “translational medicine” area. During its finalized 4 years of publication, 16 quarterly issues were published on time, covering nearly 200 articles of which at least one-third were cited by journals listed in the Web of Science. The journal seems to be very close to hit its mark and warrants to attract much more attention in the near future. In order to reach a higher scientific value, in the long run, our editorial team...
2011年初，《实验与中西医结合杂志》推出了以“从细胞到系统，从机制到疾病”为座右铭的医学文献[1]。该杂志的范围涵盖了整个生物医学研究领域，特别关注将实验数据与临床应用相结合;换句话说，就是所谓的“从实验室到病床”或“转化医学”领域。在最后出版的4年里，按时出版了16期季刊，涵盖近200篇文章，其中至少三分之一的文章被Web of Science列出的期刊引用。这本杂志似乎非常接近它的目标，并保证在不久的将来吸引更多的关注。为了达到更高的科学价值，从长远来看，我们的编辑团队……
{"title":"Starting the fifth year of publication: switching from road to highway -","authors":"S. Oter, K. Şimşek","doi":"10.5455/JEIM.300315.ED.019","DOIUrl":"https://doi.org/10.5455/JEIM.300315.ED.019","url":null,"abstract":"With the beginning of 2011, the “Journal of Experimental and Integrative Medicine” launched the medical literature with the motto “from cell to system, from mechanism to disease” [1]. The scope of the journal covered the entire field of biomedical research with particular interest on integrating experimental data to clinical applications; in other words, the so-called “bench-to-bedside” or “translational medicine” area. During its finalized 4 years of publication, 16 quarterly issues were published on time, covering nearly 200 articles of which at least one-third were cited by journals listed in the Web of Science. The journal seems to be very close to hit its mark and warrants to attract much more attention in the near future. In order to reach a higher scientific value, in the long run, our editorial team...","PeriodicalId":16091,"journal":{"name":"Journal of Experimental and Integrative Medicine","volume":"25 1","pages":"1-2"},"PeriodicalIF":0.0,"publicationDate":"2015-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84755523","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2015-01-01DOI: 10.5455/jeim.190415.or.127
Z. Çoban, S. Guran, Atmaca Şahin Sağaltıcı
Objective: Genes are regulated at multiple levels by using nutritional factors during neurogenesis and gliogenesis in brain development. Folic acid (FA)and zinc regulate the expressions of some genes which participate in brain development as nutritional factors. So, we aimed to find the effect of FA and zinc on the expression levels of neurogenin 3 (NGN3), Kruppel-like factor 4 (Klf4), c-Myc, nanog, POU class 5 homeobox 1-Pou5F1 (Oct4), and Nestin in mouse fetal brain tissue in neural plate phase at gestational day 7 (GD7)and in fetal brain tissue at GD20. Methods: All the tissues were cultured primarily. FA and zinc solutions were added. Real time-polymerase chain reaction was performed in RNA obtained from in each sample. Results: FA has no effect on GD7 mouse neural plate tissue and GD20 mouse fetal brain tissue. Zinc has no effect on GD7 mouse neural plate tissue. Zinc increased the expressions of NGN3, Klf4, nanog, Nestin, and Oct4 genes on GD20 mouse fetal brain tissue. Conclusion: This in vitro study represents that zinc is important in the expressions of NGN3, Klf4, Nanog, Nestin, and Oct4 genes in the late phase of pregnancy. The stimulator effect of Zinc on the expression levels of these genes may show us the possible role of zinc in fetal brain development in the late phase of pregnancy.
{"title":"In vitro effects of zinc and folic acid on the expressions of Neurogenin 3, Kruppel-like factor 4, c-Myc, Nanog, Nestin and POU class 5 homeobox 1 genes -","authors":"Z. Çoban, S. Guran, Atmaca Şahin Sağaltıcı","doi":"10.5455/jeim.190415.or.127","DOIUrl":"https://doi.org/10.5455/jeim.190415.or.127","url":null,"abstract":"Objective: Genes are regulated at multiple levels by using nutritional factors during neurogenesis and gliogenesis in brain development. Folic acid (FA)and zinc regulate the expressions of some genes which participate in brain development as nutritional factors. So, we aimed to find the effect of FA and zinc on the expression levels of neurogenin 3 (NGN3), Kruppel-like factor 4 (Klf4), c-Myc, nanog, POU class 5 homeobox 1-Pou5F1 (Oct4), and Nestin in mouse fetal brain tissue in neural plate phase at gestational day 7 (GD7)and in fetal brain tissue at GD20. Methods: All the tissues were cultured primarily. FA and zinc solutions were added. Real time-polymerase chain reaction was performed in RNA obtained from in each sample. Results: FA has no effect on GD7 mouse neural plate tissue and GD20 mouse fetal brain tissue. Zinc has no effect on GD7 mouse neural plate tissue. Zinc increased the expressions of NGN3, Klf4, nanog, Nestin, and Oct4 genes on GD20 mouse fetal brain tissue. Conclusion: This in vitro study represents that zinc is important in the expressions of NGN3, Klf4, Nanog, Nestin, and Oct4 genes in the late phase of pregnancy. The stimulator effect of Zinc on the expression levels of these genes may show us the possible role of zinc in fetal brain development in the late phase of pregnancy.","PeriodicalId":16091,"journal":{"name":"Journal of Experimental and Integrative Medicine","volume":"3 1","pages":"75-80"},"PeriodicalIF":0.0,"publicationDate":"2015-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91269117","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2015-01-01DOI: 10.5455/JEIM.130415.CR.006
S. Harris, Ansari Mm, Anmol Gupta, N. Afroz
Primary appendicular tuberculosis is a rare form of abdominal tuberculosis, occuring in 0.1-0.6% of the cases, and it is almost always diagnosed post-surgical on histopathology. In the present case, a 14-year-old girl underwent laparoscopic appendicectomy for acute appendicitis. During operation, only appendix and mesoappendix were found inflamed and thickened, and there was no other abnormality. Histopathology revealed tuberculosis of appendix. Anti-tuberculous treatment was started, and the patient was doing well with weight gain of 5 kg at the follow-up of 3 months.
{"title":"Isolated appendicular tuberculosis: A rare cause of acute appendicitis","authors":"S. Harris, Ansari Mm, Anmol Gupta, N. Afroz","doi":"10.5455/JEIM.130415.CR.006","DOIUrl":"https://doi.org/10.5455/JEIM.130415.CR.006","url":null,"abstract":"Primary appendicular tuberculosis is a rare form of abdominal tuberculosis, occuring in 0.1-0.6% of the cases, and it is almost always diagnosed post-surgical on histopathology. In the present case, a 14-year-old girl underwent laparoscopic appendicectomy for acute appendicitis. During operation, only appendix and mesoappendix were found inflamed and thickened, and there was no other abnormality. Histopathology revealed tuberculosis of appendix. Anti-tuberculous treatment was started, and the patient was doing well with weight gain of 5 kg at the follow-up of 3 months.","PeriodicalId":16091,"journal":{"name":"Journal of Experimental and Integrative Medicine","volume":"13 1","pages":"114-116"},"PeriodicalIF":0.0,"publicationDate":"2015-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81708641","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2015-01-01DOI: 10.5455/JEIM.160615.RW.013
P. Szachta, Anna Bartnicka, M. Gałęcka, K. Skonieczna-Żydecka
Pathogenesis of autism spectrum disorders (ASD) is probably multifactorial. Many studies have shown intestinal dysbiosis in children with autism. Moreover, gastrointestinal disturbances, probably resulting from abnormal microbiota composition, are commonly reported in children with autism. Another aspect, that may have negative influence on ASD children’s behavior are enzymopathies (enzymatic deficiency). Lack of selected gastrointestinal enzymes leads to inappropriate nutrients decomposition (mainly gluten and casein) and formation of so called exorphins, i.e. substances with opioid activity (opioid therapy). Because of increased permeability of the intestinal barrier and cerebrospinal axis, exorphins and others improperly digested food particles may be transported to the central nervous system. Consequently, disorders in brain development and children’s behavior deterioration may be observed. Studies have shown that both microflora disorders and increased intestinal permeability may contribute to behavioral impairment.
{"title":"Microbiota disorders and food hypersensitivity in autism spectrum disorders; what do we know?","authors":"P. Szachta, Anna Bartnicka, M. Gałęcka, K. Skonieczna-Żydecka","doi":"10.5455/JEIM.160615.RW.013","DOIUrl":"https://doi.org/10.5455/JEIM.160615.RW.013","url":null,"abstract":"Pathogenesis of autism spectrum disorders (ASD) is probably multifactorial. Many studies have shown intestinal dysbiosis in children with autism. Moreover, gastrointestinal disturbances, probably resulting from abnormal microbiota composition, are commonly reported in children with autism. Another aspect, that may have negative influence on ASD children’s behavior are enzymopathies (enzymatic deficiency). Lack of selected gastrointestinal enzymes leads to inappropriate nutrients decomposition (mainly gluten and casein) and formation of so called exorphins, i.e. substances with opioid activity (opioid therapy). Because of increased permeability of the intestinal barrier and cerebrospinal axis, exorphins and others improperly digested food particles may be transported to the central nervous system. Consequently, disorders in brain development and children’s behavior deterioration may be observed. Studies have shown that both microflora disorders and increased intestinal permeability may contribute to behavioral impairment.","PeriodicalId":16091,"journal":{"name":"Journal of Experimental and Integrative Medicine","volume":"16 1","pages":"117-120"},"PeriodicalIF":0.0,"publicationDate":"2015-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83321317","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2015-01-01DOI: 10.5455/JEIM.200515.OR.132
Sushma R Kotian, A. Sinha, A. S. Souza, S. Sumalatha
Objective: The sciatic nerve, a largest branch of the sacral plexus is prone to variations in its formation, course and branching pattern which may lead to multiple clinical complaints. The aim of the present study was to analyze the incidence of its variations in South Indian population. Methods: 60 gluteal regions were examined in 30 formalin fixed adult cadavers for the variations in the formation, termination of the sciatic nerve and its relationship with the piriformis muscle. Results: The sciatic nerve showed variations in its formation and as well as termination. High division of the sciatic nerve was the most commonly encountered variation (45%). The relationship of the sciatic nerve and its branches with the piriformis muscle was also variable. In majority of the cases (56.67%), the sciatic nerve and its branches were passing inferior to piriformis. Conclusion: Although the variations of the sciatic nerve are common and are already reported, the present study indicates some additional findings not reported previously and further stresses on its applied significance. These variations are important for surgeons, as this is an area of frequent surgical manipulation, nerve injury during deep intramuscular injections, sciatic nerve block etc. They may lead to in-advertent injury during operations, piriformis syndrome, non-discogenic sciatica, muscle atrophy, failure of sciatic nerve block and many other complications.
{"title":"Variations of the sciatic nerve and its relation with the piriformis muscle in South Indian population","authors":"Sushma R Kotian, A. Sinha, A. S. Souza, S. Sumalatha","doi":"10.5455/JEIM.200515.OR.132","DOIUrl":"https://doi.org/10.5455/JEIM.200515.OR.132","url":null,"abstract":"Objective: The sciatic nerve, a largest branch of the sacral plexus is prone to variations in its formation, course and branching pattern which may lead to multiple clinical complaints. The aim of the present study was to analyze the incidence of its variations in South Indian population. Methods: 60 gluteal regions were examined in 30 formalin fixed adult cadavers for the variations in the formation, termination of the sciatic nerve and its relationship with the piriformis muscle. Results: The sciatic nerve showed variations in its formation and as well as termination. High division of the sciatic nerve was the most commonly encountered variation (45%). The relationship of the sciatic nerve and its branches with the piriformis muscle was also variable. In majority of the cases (56.67%), the sciatic nerve and its branches were passing inferior to piriformis. Conclusion: Although the variations of the sciatic nerve are common and are already reported, the present study indicates some additional findings not reported previously and further stresses on its applied significance. These variations are important for surgeons, as this is an area of frequent surgical manipulation, nerve injury during deep intramuscular injections, sciatic nerve block etc. They may lead to in-advertent injury during operations, piriformis syndrome, non-discogenic sciatica, muscle atrophy, failure of sciatic nerve block and many other complications.","PeriodicalId":16091,"journal":{"name":"Journal of Experimental and Integrative Medicine","volume":"1995 1","pages":"144-148"},"PeriodicalIF":0.0,"publicationDate":"2015-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82443648","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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