Pub Date : 2016-01-01DOI: 10.5455/jeim.150816.or.156
O. B. Eze, O. Nwodo
Abstract Aim: This study aims to evaluate the membrane stability, Phospholipase A2 activity, prostaglandin synthase activity and aggregatory effect of ethanol leaves extract of Sida acuta Burm F. using an in-vitro heamolytic assay. Materials and methods: Two milliliters of blood collected from a healthy volunteer who has not taken drugs for last two weeks and ethanol extracts leaves of Sida acuta were used in the assay. Results: Apparently there was platelet aggregation at all but there was no minimal aggregation to denote in samples 1 and 2. There was a decrease in the supernatants of sample 1 of hypotonicity-induced haemolysis of human red blood cells incubated in normal saline but there was a high increase in the supernatants of sample 2 incubated with hypotonic solution. Inclusion of the extract concentrations 2, 4 and 8 mg/ml in the incubation medium (hypotonic solution) in samples 3, 4 and 5 decreased in a concentration dependent fashion. The absorption of haemolysed human red blood cells in isotonic solution showed the OD of 540nm:630nm to 2.15 for methaemoglobin while the OD of 540nm:570nm was 0.97 for deoxyhaemoglobin. In hypotonic solution where haemoglobin was released, the ratio of 540nm:630nm was 4.41 and 540nm:570nm was 1.20. The inclusion of the extract concentrations 2, 4 and 8 mg/ml in samples 3, 4 and 5 caused the ratio to decreased from1.69, 1.58 and 0.41 for methaemoglobin and 1.08, 0.86 and 0.41 for deoxyhaemoglobin. There was a decrease in activity of phospholipase A2 in samples 3, 4, 5 and 9 with the extract concentrations 2, 6, 12 and 16 mg/ml respectively but no decrease was observed in samples 6,7, 8 and 10, though no human red blood cells was included in samples 6, 7, 8, 9 and 10. There was a significant reduction (p 0.05) in prostaglandin synthase activity in sample 4 with extract concentration 25 mg/ml but a non-significant decrease (p 0.05) was observed in samples 3 and 5. Conclusion: The extract showed a significant inhibition of in-vitro haemolysis and could have a potential therapeutic effect on disease processes causing destabilization of biological membranes.
{"title":"in-vitro stability and aggregatory effect of ethanol extract leaves of Sida acuta Burm F. on human erythrocyte. -","authors":"O. B. Eze, O. Nwodo","doi":"10.5455/jeim.150816.or.156","DOIUrl":"https://doi.org/10.5455/jeim.150816.or.156","url":null,"abstract":"Abstract Aim: This study aims to evaluate the membrane stability, Phospholipase A2 activity, prostaglandin synthase activity and aggregatory effect of ethanol leaves extract of Sida acuta Burm F. using an in-vitro heamolytic assay. Materials and methods: Two milliliters of blood collected from a healthy volunteer who has not taken drugs for last two weeks and ethanol extracts leaves of Sida acuta were used in the assay. Results: Apparently there was platelet aggregation at all but there was no minimal aggregation to denote in samples 1 and 2. There was a decrease in the supernatants of sample 1 of hypotonicity-induced haemolysis of human red blood cells incubated in normal saline but there was a high increase in the supernatants of sample 2 incubated with hypotonic solution. Inclusion of the extract concentrations 2, 4 and 8 mg/ml in the incubation medium (hypotonic solution) in samples 3, 4 and 5 decreased in a concentration dependent fashion. The absorption of haemolysed human red blood cells in isotonic solution showed the OD of 540nm:630nm to 2.15 for methaemoglobin while the OD of 540nm:570nm was 0.97 for deoxyhaemoglobin. In hypotonic solution where haemoglobin was released, the ratio of 540nm:630nm was 4.41 and 540nm:570nm was 1.20. The inclusion of the extract concentrations 2, 4 and 8 mg/ml in samples 3, 4 and 5 caused the ratio to decreased from1.69, 1.58 and 0.41 for methaemoglobin and 1.08, 0.86 and 0.41 for deoxyhaemoglobin. There was a decrease in activity of phospholipase A2 in samples 3, 4, 5 and 9 with the extract concentrations 2, 6, 12 and 16 mg/ml respectively but no decrease was observed in samples 6,7, 8 and 10, though no human red blood cells was included in samples 6, 7, 8, 9 and 10. There was a significant reduction (p 0.05) in prostaglandin synthase activity in sample 4 with extract concentration 25 mg/ml but a non-significant decrease (p 0.05) was observed in samples 3 and 5. Conclusion: The extract showed a significant inhibition of in-vitro haemolysis and could have a potential therapeutic effect on disease processes causing destabilization of biological membranes.","PeriodicalId":16091,"journal":{"name":"Journal of Experimental and Integrative Medicine","volume":"26 1","pages":"134-138"},"PeriodicalIF":0.0,"publicationDate":"2016-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86281334","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2016-01-01DOI: 10.5455/jeim.060916.or.159
G. Anyasor, O. Adeyemi
Objective: This study investigated the effects of oral co-administered ascorbic acid (AA) and dihydroartemisinin (DHA) on some hepatotoxic biomarkers and parasitaemia counts in Plasmodium berghei Anka strain infected mice for 7 d. Methods: Twenty four male Swiss albino mice were randomly distributed into six groups; group I: “non-parasitized and non-treated”(nPnT), group II: “parasitized and non-treated”(PnT), group III: parasitized mice administered 5 mg/kg b.w. DHA, group IV: parasitized mice administered 5 mg/kg b.w. AA, group V: parasitized mice co-administered 5 mg/kg b.w. DHA+ 5 mg/kg b.w. AA and group VI: parasitized mice administered 25 mg/kg b.w. chloroquine (CQ) as standard. Results: Plasma alanine aminotransferase (ALT) and aspartate aminotransferase (AST) activities were significantly (P0.05) different. However, DHA and CQ treated mice had significantly reduced parasite count/μl blood at P
{"title":"Oral administered ascorbic acid attenuated dihydroartemisinin anti-plasmodial activity and elicited hepatic injury in Plasmodium berghei strain Anka infected mice -","authors":"G. Anyasor, O. Adeyemi","doi":"10.5455/jeim.060916.or.159","DOIUrl":"https://doi.org/10.5455/jeim.060916.or.159","url":null,"abstract":"Objective: This study investigated the effects of oral co-administered ascorbic acid (AA) and dihydroartemisinin (DHA) on some hepatotoxic biomarkers and parasitaemia counts in Plasmodium berghei Anka strain infected mice for 7 d. Methods: Twenty four male Swiss albino mice were randomly distributed into six groups; group I: “non-parasitized and non-treated”(nPnT), group II: “parasitized and non-treated”(PnT), group III: parasitized mice administered 5 mg/kg b.w. DHA, group IV: parasitized mice administered 5 mg/kg b.w. AA, group V: parasitized mice co-administered 5 mg/kg b.w. DHA+ 5 mg/kg b.w. AA and group VI: parasitized mice administered 25 mg/kg b.w. chloroquine (CQ) as standard. Results: Plasma alanine aminotransferase (ALT) and aspartate aminotransferase (AST) activities were significantly (P0.05) different. However, DHA and CQ treated mice had significantly reduced parasite count/μl blood at P","PeriodicalId":16091,"journal":{"name":"Journal of Experimental and Integrative Medicine","volume":"8 1","pages":"139-142"},"PeriodicalIF":0.0,"publicationDate":"2016-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84570116","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2016-01-01DOI: 10.5455/JEIM.280516.OR.150
ukpabiugo ukpabiugo, J. Chigozie, P. Iwuanyanwu, kingsley Chukwuemeka, Monanu Okechukwu
Objective: The present study is aimed at evaluating the antioxidant activity of different solvent fractions (ethylacetate, ethanol, hexane, aqueous) of Ocimum gratissimum (OG) on oxidative stress indices in Paracetamol-induced oxidative stress in Wistar albino rats. Methods: Thirty five Wistar albino rats of both sexes were divided into seven groups (n=5). Paracetamol (PCM) (500mg/ kg BW) dissolved in olive oil was administered daily for 28 days to induce hepatic damage in experimental rats. Control group received 1 ml olive oil only. 400 mg/kg BW doses of ethyl acetate, ethanol, hexane, aqueous fractions of OG and silymarin (SIL) (100mg/kg) in addition to 500mg/kg body weight of PCM dissolved in 1ml of olive oil respectively were used as treatment groups daily for 28 days. Blood samples were collected by cardiac puncture and activities of Superoxide dismutase (SOD), catalase (CAT), glutathione (GSH) using standard biochemical methods. Lipid peroxidation was estimated by measuring Malondialdehyde (MDA) concentration. Results: SOD activity increased significantly (p≤ 0.05) in groups administered different fractions of OG and SIL when compared with control. CAT activity showed significant (p≤ 0.05) increase in ethanol and ethyl acetate fractions of OG while group treated with SIL and groups administered hexane and aqueous fractions showed a marked increase when compared with rats treated with PCM only. Reduced GSH level increased significantly (p≤ 0.05) in aqueous fraction while SIL-treated group and groups administered fractions of OG (ethyl acetate, ethanol and hexane) showed a marked increase when compared with PCM only treated group. MDA concentration decreased significantly (p≤ 0.05) in groups administered different fractions of OG when compared with PCM only treated group. Conclusion: These findings are suggestive of the antioxidant properties of different solvent fractions of Ocimum gratissimum extract in paracetamol-induced oxidative stress with ethyl acetate fraction showing the highest activity.
目的:研究不同溶剂组分(乙酸乙酯、乙醇、己烷、水溶液)对白化Wistar大鼠对乙酰氨基酚诱导氧化应激的抗氧化活性。方法:35只Wistar白化大鼠，雌雄同体，随机分为7组(n=5)。对乙酰氨基酚(Paracetamol, PCM) (500mg/ kg BW)溶解于橄榄油中，每天给药28 d，以诱导实验大鼠肝损伤。对照组仅给予橄榄油1 ml。以乙酸乙酯、乙醇、己烷、OG和水飞蓟素(SIL)水溶液(100mg/kg) 400 mg/kg剂量和500mg/kg体重的PCM分别溶于1ml橄榄油作为处理组，连续28 d。采用心脏穿刺法采集血样，采用标准生化方法检测超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、谷胱甘肽(GSH)活性。脂质过氧化通过测定丙二醛(MDA)浓度来估计。结果:与对照组相比，OG、SIL不同部位组SOD活性均显著升高(p≤0.05)。与单纯PCM组相比，OG乙醇组和乙酸乙酯组、己烷组和水组的CAT活性显著(p≤0.05)升高。水段还原性GSH水平显著升高(p≤0.05)，而硅油处理组和OG组分(乙酸乙酯、乙醇和己烷)处理组与单纯PCM处理组相比显著升高。与单用PCM处理组相比，不同部位OG处理组MDA浓度显著降低(p≤0.05)。结论:不同溶剂组分对扑热息痛诱导的氧化应激均有一定的抗氧化作用，其中乙酸乙酯组分的抗氧化活性最高。
{"title":"Antioxidant properties of ethanol and fractions of ocimum gratissimum leave in paracetamol-induced oxidative stress in Wistar albino rats. -","authors":"ukpabiugo ukpabiugo, J. Chigozie, P. Iwuanyanwu, kingsley Chukwuemeka, Monanu Okechukwu","doi":"10.5455/JEIM.280516.OR.150","DOIUrl":"https://doi.org/10.5455/JEIM.280516.OR.150","url":null,"abstract":"Objective: The present study is aimed at evaluating the antioxidant activity of different solvent fractions (ethylacetate, ethanol, hexane, aqueous) of Ocimum gratissimum (OG) on oxidative stress indices in Paracetamol-induced oxidative stress in Wistar albino rats. Methods: Thirty five Wistar albino rats of both sexes were divided into seven groups (n=5). Paracetamol (PCM) (500mg/ kg BW) dissolved in olive oil was administered daily for 28 days to induce hepatic damage in experimental rats. Control group received 1 ml olive oil only. 400 mg/kg BW doses of ethyl acetate, ethanol, hexane, aqueous fractions of OG and silymarin (SIL) (100mg/kg) in addition to 500mg/kg body weight of PCM dissolved in 1ml of olive oil respectively were used as treatment groups daily for 28 days. Blood samples were collected by cardiac puncture and activities of Superoxide dismutase (SOD), catalase (CAT), glutathione (GSH) using standard biochemical methods. Lipid peroxidation was estimated by measuring Malondialdehyde (MDA) concentration. Results: SOD activity increased significantly (p≤ 0.05) in groups administered different fractions of OG and SIL when compared with control. CAT activity showed significant (p≤ 0.05) increase in ethanol and ethyl acetate fractions of OG while group treated with SIL and groups administered hexane and aqueous fractions showed a marked increase when compared with rats treated with PCM only. Reduced GSH level increased significantly (p≤ 0.05) in aqueous fraction while SIL-treated group and groups administered fractions of OG (ethyl acetate, ethanol and hexane) showed a marked increase when compared with PCM only treated group. MDA concentration decreased significantly (p≤ 0.05) in groups administered different fractions of OG when compared with PCM only treated group. Conclusion: These findings are suggestive of the antioxidant properties of different solvent fractions of Ocimum gratissimum extract in paracetamol-induced oxidative stress with ethyl acetate fraction showing the highest activity.","PeriodicalId":16091,"journal":{"name":"Journal of Experimental and Integrative Medicine","volume":"26 1","pages":"66-70"},"PeriodicalIF":0.0,"publicationDate":"2016-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84061913","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2016-01-01DOI: 10.5455/JEIM.310816.OR.158
S. Saheed, Ashafa Anofi Omotayo Tom
{"title":"Evaluation of daily double dose administration of ethanolic root extract of Morella serrata (Lam.) Killick in rats","authors":"S. Saheed, Ashafa Anofi Omotayo Tom","doi":"10.5455/JEIM.310816.OR.158","DOIUrl":"https://doi.org/10.5455/JEIM.310816.OR.158","url":null,"abstract":"","PeriodicalId":16091,"journal":{"name":"Journal of Experimental and Integrative Medicine","volume":"6 1","pages":"109-117"},"PeriodicalIF":0.0,"publicationDate":"2016-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86026176","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2016-01-01DOI: 10.5455/JEIM.121216.OR.165
K. Mireille, D. Désiré, Bilanda Danielle Claude, M. N. Y. Sandrine, Mballa Marguerite Francine, Ngoungoure Madeleine Chantal, O. Carolle, D. Théophile, Kamtchouing Pierre
To investigate Nymphaea lotus aqueous extract on L-NAME-mediated sexual dysfunction and tissular oxidative stress in male Wistar rats. Methods: Fifty adult male Wistar rats were randomly classified into 5 groups; Control, L-NAME (10 mg/kg), L-NAME + losartan (10 mg/kg), L-NAME + N. lotus at the dose of 75 mg/kg and 200 mg/kg. L-NAME was administered during 8 weeks, but others treatments started from the 4th week and were administered continuously with L-NAME (10 mg/kg) during 4 additional weeks. Results: L-NAME administration caused marked hit of sexual behaviour, specifically failure of penile insertion or difficulty to ejaculate during the test interval. Further L-NAME causes a significant decrease in antioxidant products as reduced gluthatione (GSH) and nitrites (NO) in aorta and penile tissues, as compared to control group. N. lotus co-treatment for 4 weeks increased markedly the erectile index and the ejaculation rates contrarily to losartan in comparison to negative control receiving only L-NAME. N. lotus, but not the positive drug losartan, induced a significant increase in the anti-oxidant enzymes activities and GSH and NO levels, as well as a significant decrease in MDA levels compared to L-NAME group. These results suggests N. lotus may provide significant protection against L-NAME-induced tissular oxidative damages by up-regulation of antioxidant systems and promotion of the vasodilator factors in chronic NO-deficient rats. These alleviating effects of Nymphaea lotus denote a pro-sexual, antioxidant and vasodilatatory properties that was more appreciated after histological examination where remodeling of aorta were visibly reduced. Conclusion: N. lotus may be considered as a potentially useful strategy to limit erectile dysfunction and toxicity associated with hypertension.
{"title":"Protective effects of Nymphaea lotus Linn (Nymphaeaceae) on L-NAME-induced tissular oxidative damages and erectile dysfunction in hypertensive male rat","authors":"K. Mireille, D. Désiré, Bilanda Danielle Claude, M. N. Y. Sandrine, Mballa Marguerite Francine, Ngoungoure Madeleine Chantal, O. Carolle, D. Théophile, Kamtchouing Pierre","doi":"10.5455/JEIM.121216.OR.165","DOIUrl":"https://doi.org/10.5455/JEIM.121216.OR.165","url":null,"abstract":"To investigate Nymphaea lotus aqueous extract on L-NAME-mediated sexual dysfunction and tissular oxidative stress in male Wistar rats. Methods: Fifty adult male Wistar rats were randomly classified into 5 groups; Control, L-NAME (10 mg/kg), L-NAME + losartan (10 mg/kg), L-NAME + N. lotus at the dose of 75 mg/kg and 200 mg/kg. L-NAME was administered during 8 weeks, but others treatments started from the 4th week and were administered continuously with L-NAME (10 mg/kg) during 4 additional weeks. Results: L-NAME administration caused marked hit of sexual behaviour, specifically failure of penile insertion or difficulty to ejaculate during the test interval. Further L-NAME causes a significant decrease in antioxidant products as reduced gluthatione (GSH) and nitrites (NO) in aorta and penile tissues, as compared to control group. N. lotus co-treatment for 4 weeks increased markedly the erectile index and the ejaculation rates contrarily to losartan in comparison to negative control receiving only L-NAME. N. lotus, but not the positive drug losartan, induced a significant increase in the anti-oxidant enzymes activities and GSH and NO levels, as well as a significant decrease in MDA levels compared to L-NAME group. These results suggests N. lotus may provide significant protection against L-NAME-induced tissular oxidative damages by up-regulation of antioxidant systems and promotion of the vasodilator factors in chronic NO-deficient rats. These alleviating effects of Nymphaea lotus denote a pro-sexual, antioxidant and vasodilatatory properties that was more appreciated after histological examination where remodeling of aorta were visibly reduced. Conclusion: N. lotus may be considered as a potentially useful strategy to limit erectile dysfunction and toxicity associated with hypertension.","PeriodicalId":16091,"journal":{"name":"Journal of Experimental and Integrative Medicine","volume":"40 9 1","pages":"178-184"},"PeriodicalIF":0.0,"publicationDate":"2016-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82849120","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2016-01-01DOI: 10.5455/JEIM.170316.CR.007
A. Akhtar, P. Pandey, R. Tanwar, A. Ahuja, R. Sood
Squamous cell carcinoma (SCC) of renal pelvis is a subtle tumor constituting only about 0.7% to 7% of all renal malignancies. Out of various aetiologies proposed, the most pertinent is long standing irritation due to calculi and infection. Pyelo-entero-cutaneous fistula is a rare but serious condition mostly secondary to iatrogenic injury occurring during per-cutaneous renal surgeries.We report a rare case of Squamous cell carcinoma of the renal pelvis presenting as Pyelo-entero-Cutaneous Fistula. The diagnosis of SCC of renal pelvis was made on post surgery pathological examination.
{"title":"Squamous cell carcinoma of renal pelvis presenting as pyelo-entero-cutaneous fistula: Case report and review of literature -","authors":"A. Akhtar, P. Pandey, R. Tanwar, A. Ahuja, R. Sood","doi":"10.5455/JEIM.170316.CR.007","DOIUrl":"https://doi.org/10.5455/JEIM.170316.CR.007","url":null,"abstract":"Squamous cell carcinoma (SCC) of renal pelvis is a subtle tumor constituting only about 0.7% to 7% of all renal malignancies. Out of various aetiologies proposed, the most pertinent is long standing irritation due to calculi and infection. Pyelo-entero-cutaneous fistula is a rare but serious condition mostly secondary to iatrogenic injury occurring during per-cutaneous renal surgeries.We report a rare case of Squamous cell carcinoma of the renal pelvis presenting as Pyelo-entero-Cutaneous Fistula. The diagnosis of SCC of renal pelvis was made on post surgery pathological examination.","PeriodicalId":16091,"journal":{"name":"Journal of Experimental and Integrative Medicine","volume":"1 1","pages":"49-51"},"PeriodicalIF":0.0,"publicationDate":"2016-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80547675","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2016-01-01DOI: 10.5455/JEIM.140316.OR.148
S. Sabiu, E. Ajani, Bamishile Oladipo Temitope, Yinusa Omowunmi Bashirat
Objective: To investigate Cyperus esculentus aqueous extract on paroxetine-mediated sexual dysfunction in male Wistar rats. Methods: Sexually dysfunctioned (SD) rats were orally treated with PowMaxM [reference drug (7.14 mg/kg)] and the extract (500 and 1000 mg/kg body weight) once daily for 10 days, and their sexual behavioural parameters were monitored and computed. Relative testes-body weight and testicular function parameters were also evaluated at the end of the treatment period. Results: Dim light observation on the animals revealed respective proceptive and precopulatory behaviours by the primed female animals and the extract-treated male rats. Compared with SD rats, the extract-treated groups significantly restored and improved sexual behaviour and libido as evident from the remarkably increased frequencies of mount, intromission, ejaculation as well as the significantly reduced latencies of mount, intromission, ejaculation and post-ejaculation. The significantly reduced testicular activities of alkaline phosphatase, acid phosphatase, lactate dehydrogenase and the concentrations of protein, cholesterol, glycogen, testosterone, luteinizing hormone, follicle stimulating hormone and testes-body weight in the SD rats were also markedly increased following treatment with the extract. The effects elicited by the extract competed favourably with the reference drug used. The improved sexual competences exhibited by the male rats in this study are indicative of aphrodisiac attributes of the extract and could be adduced to the presence of phytonutrients as revealed by the GC-MS chromatogram. Conclusion: The data from this study suggest that C. esculentus is capable of restoring and boosting sexual competence and the probable mechanism is via enhancement of adaptogenic bioactive principles.
{"title":"Aqueous extract of Cyperus esculentus L. restores and boosts sexual competence in paroxetine-dysfunctioned male Wistar rats -","authors":"S. Sabiu, E. Ajani, Bamishile Oladipo Temitope, Yinusa Omowunmi Bashirat","doi":"10.5455/JEIM.140316.OR.148","DOIUrl":"https://doi.org/10.5455/JEIM.140316.OR.148","url":null,"abstract":"Objective: To investigate Cyperus esculentus aqueous extract on paroxetine-mediated sexual dysfunction in male Wistar rats. Methods: Sexually dysfunctioned (SD) rats were orally treated with PowMaxM [reference drug (7.14 mg/kg)] and the extract (500 and 1000 mg/kg body weight) once daily for 10 days, and their sexual behavioural parameters were monitored and computed. Relative testes-body weight and testicular function parameters were also evaluated at the end of the treatment period. Results: Dim light observation on the animals revealed respective proceptive and precopulatory behaviours by the primed female animals and the extract-treated male rats. Compared with SD rats, the extract-treated groups significantly restored and improved sexual behaviour and libido as evident from the remarkably increased frequencies of mount, intromission, ejaculation as well as the significantly reduced latencies of mount, intromission, ejaculation and post-ejaculation. The significantly reduced testicular activities of alkaline phosphatase, acid phosphatase, lactate dehydrogenase and the concentrations of protein, cholesterol, glycogen, testosterone, luteinizing hormone, follicle stimulating hormone and testes-body weight in the SD rats were also markedly increased following treatment with the extract. The effects elicited by the extract competed favourably with the reference drug used. The improved sexual competences exhibited by the male rats in this study are indicative of aphrodisiac attributes of the extract and could be adduced to the presence of phytonutrients as revealed by the GC-MS chromatogram. Conclusion: The data from this study suggest that C. esculentus is capable of restoring and boosting sexual competence and the probable mechanism is via enhancement of adaptogenic bioactive principles.","PeriodicalId":16091,"journal":{"name":"Journal of Experimental and Integrative Medicine","volume":"45 1","pages":"12-20"},"PeriodicalIF":0.0,"publicationDate":"2016-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77898316","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2016-01-01DOI: 10.5455/JEIM.111016.OR.163
Leela Krishna Vatsavai, E. Kilari
{"title":"Effect of fucoxanthin on the pharmacodynamics and pharmacokinetics of gliclazide in animal models","authors":"Leela Krishna Vatsavai, E. Kilari","doi":"10.5455/JEIM.111016.OR.163","DOIUrl":"https://doi.org/10.5455/JEIM.111016.OR.163","url":null,"abstract":"","PeriodicalId":16091,"journal":{"name":"Journal of Experimental and Integrative Medicine","volume":"16 1","pages":"172"},"PeriodicalIF":0.0,"publicationDate":"2016-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86416884","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2016-01-01DOI: 10.5455/JEIM.260616.OR.152
Y. Yıldız, A. Apaydın, M. M. Seven, Aydan Örsçelik
Objective: Prolotherapy is recognized as a regenerative injection technique that stimulates the body’s healing processes to strengthen weakened ligaments and tendons. Hypertonic dextrose as an irritant agent which stimulates the healing process agent is widely used in in this technique. The aim of the current study is to assess the efficiency of prolotherapy injections in patellofemoral pain syndrome Methods: The current study was designed as a retrospective clinical study. 44 recreational athletes with unilateral PFPS were enrolled in this study. 10 ml of 15% and 5 ml of 25% hypertonic dextrose were used as extra- and intra-articular injection in the knee with PFPS respectively. Extra and intra articular injections were repeated three times with 21 day intervals according to the tissue repair processing. Results: The knee functional scores, balance and coordination scores were significantly improved in the affected knees at 6 months after treatment (p
{"title":"The Effects of Prolotherapy (Hypertonic Dextrose) in Recreational Athletes with Patellofemoral Pain Syndrome","authors":"Y. Yıldız, A. Apaydın, M. M. Seven, Aydan Örsçelik","doi":"10.5455/JEIM.260616.OR.152","DOIUrl":"https://doi.org/10.5455/JEIM.260616.OR.152","url":null,"abstract":"Objective: Prolotherapy is recognized as a regenerative injection technique that stimulates the body’s healing processes to strengthen weakened ligaments and tendons. Hypertonic dextrose as an irritant agent which stimulates the healing process agent is widely used in in this technique. The aim of the current study is to assess the efficiency of prolotherapy injections in patellofemoral pain syndrome Methods: The current study was designed as a retrospective clinical study. 44 recreational athletes with unilateral PFPS were enrolled in this study. 10 ml of 15% and 5 ml of 25% hypertonic dextrose were used as extra- and intra-articular injection in the knee with PFPS respectively. Extra and intra articular injections were repeated three times with 21 day intervals according to the tissue repair processing. Results: The knee functional scores, balance and coordination scores were significantly improved in the affected knees at 6 months after treatment (p","PeriodicalId":16091,"journal":{"name":"Journal of Experimental and Integrative Medicine","volume":"15 1","pages":"53-56"},"PeriodicalIF":0.0,"publicationDate":"2016-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84703175","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2016-01-01DOI: 10.5455/jeim.191016.or.162
O. Ojiako, PAUL CHIDOKA CHIKEZIE, D. I. Ukairo, C. Ibegbulem, R. Nwaoguikpe
{"title":"Radical Scavenging Potential: Antioxidant Vitamins and Total Phenolics Contents of Raw and Hydrothermal Processed Herbs In Vitro","authors":"O. Ojiako, PAUL CHIDOKA CHIKEZIE, D. I. Ukairo, C. Ibegbulem, R. Nwaoguikpe","doi":"10.5455/jeim.191016.or.162","DOIUrl":"https://doi.org/10.5455/jeim.191016.or.162","url":null,"abstract":"","PeriodicalId":16091,"journal":{"name":"Journal of Experimental and Integrative Medicine","volume":"48 1","pages":"158"},"PeriodicalIF":0.0,"publicationDate":"2016-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78633643","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
京公网安备 11010802042870号
京ICP备2023020795号-1
扫码关注我们
求助内容：
应助结果提醒方式：