Pub Date : 2015-01-01DOI: 10.5455/JEIM.290115.RW.010
Paula R. S. Camara
The inflammatory mediators can alter the integrity of mucosa by effectively influencing the various components of the mucosal defense, which are a combination of factors that allow the mucosa withstand exposure to substances with different types of pH range, temperature, and osmosis, solutions with detergent properties (bile) and bacterial products that can activate local and systemic inflammatory reactions. On the other hand, our previous research showed that different kinds of intragastric pH range can protect or induce gastric damage in portal hypertensive rats. When damage occurs to the mucous membrane, repair of the lesion can be activated quickly, limiting the possibility of harmful substances (bacterial products) coming into contact with the systemic circulation. The resistance of the gastric mucosa to injury depends, ultimately, on a balance between defensive factors and aggressive factors present in the lumen of the mucosa. Various components of mucosal defense can be influenced by inflammatory mediators. Some of these mediators are prostanoids, nitric oxide, endothelins, cytokines, free radicals, neuropeptides and Helicobacter pylori. These mediators contribute to both the modulation of mucosal defense and the pathogenesis of gastric mucosal injury, and will be described a little more detail in the present paper.
{"title":"Endogenous vasoactive mediators in defense of portal hypertensive gastric mucosa","authors":"Paula R. S. Camara","doi":"10.5455/JEIM.290115.RW.010","DOIUrl":"https://doi.org/10.5455/JEIM.290115.RW.010","url":null,"abstract":"The inflammatory mediators can alter the integrity of mucosa by effectively influencing the various components of the mucosal defense, which are a combination of factors that allow the mucosa withstand exposure to substances with different types of pH range, temperature, and osmosis, solutions with detergent properties (bile) and bacterial products that can activate local and systemic inflammatory reactions. On the other hand, our previous research showed that different kinds of intragastric pH range can protect or induce gastric damage in portal hypertensive rats. When damage occurs to the mucous membrane, repair of the lesion can be activated quickly, limiting the possibility of harmful substances (bacterial products) coming into contact with the systemic circulation. The resistance of the gastric mucosa to injury depends, ultimately, on a balance between defensive factors and aggressive factors present in the lumen of the mucosa. Various components of mucosal defense can be influenced by inflammatory mediators. Some of these mediators are prostanoids, nitric oxide, endothelins, cytokines, free radicals, neuropeptides and Helicobacter pylori. These mediators contribute to both the modulation of mucosal defense and the pathogenesis of gastric mucosal injury, and will be described a little more detail in the present paper.","PeriodicalId":16091,"journal":{"name":"Journal of Experimental and Integrative Medicine","volume":"9 1","pages":"3-15"},"PeriodicalIF":0.0,"publicationDate":"2015-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90341917","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2015-01-01DOI: 10.5455/jeim.100515.or.130
D. L. Aleixo, F. Ferraz, L. Ciupa, W. N. S. Rodrigues, Katiucha Rebeca Jennifer Lopes Lera, M. Benvenutti, S. M. Araújo
Objective: The aim of this study was to evaluate the effect of a biotherapeutic in very low dynamization (3dH) in murine infection by Trypanosoma cruzi. Methods: Swiss male mice (Mus musculus) infected by T. cruzi Y strain were divided into three groups according to their treatment: Control infection - 7% ethanolwater solution; 3dH each day (ED) - daily biotherapeutic T. cruzi 3dH, from the day of infection until the end of experiment; 3dH single day (SD) - biotherapeutic T. cruzi 3dH, on the day of infection for 12 h. Parasitological (total parasitemia, peak of parasites, day of the peaks, prepatent period, patent period, and survival) and clinical (body temperature and body weight) parameters were evaluated. Results: No significant differences were observed among groups for parasitological evaluation. 3dH ED group presented an earlier mortality compared to control. Clinical parameters showed that animals treated with biotherapeutic had worse outcome when compared with control animals. Conclusion: The low dynamization of T. cruzi biotherapeutic worsened its murine infection by means of clinical evaluation. Considering other studies using biotherapeutic of T. cruzi, suggests the possibility for an efficacy of different dynamizations regarding oscillatory potency-effect-curves.
{"title":"Randomized study using biotherapeutic “T.cruzi 3dH” impairs experimental infection by Trypanosoma cruzi -","authors":"D. L. Aleixo, F. Ferraz, L. Ciupa, W. N. S. Rodrigues, Katiucha Rebeca Jennifer Lopes Lera, M. Benvenutti, S. M. Araújo","doi":"10.5455/jeim.100515.or.130","DOIUrl":"https://doi.org/10.5455/jeim.100515.or.130","url":null,"abstract":"Objective: The aim of this study was to evaluate the effect of a biotherapeutic in very low dynamization (3dH) in murine infection by Trypanosoma cruzi. Methods: Swiss male mice (Mus musculus) infected by T. cruzi Y strain were divided into three groups according to their treatment: Control infection - 7% ethanolwater solution; 3dH each day (ED) - daily biotherapeutic T. cruzi 3dH, from the day of infection until the end of experiment; 3dH single day (SD) - biotherapeutic T. cruzi 3dH, on the day of infection for 12 h. Parasitological (total parasitemia, peak of parasites, day of the peaks, prepatent period, patent period, and survival) and clinical (body temperature and body weight) parameters were evaluated. Results: No significant differences were observed among groups for parasitological evaluation. 3dH ED group presented an earlier mortality compared to control. Clinical parameters showed that animals treated with biotherapeutic had worse outcome when compared with control animals. Conclusion: The low dynamization of T. cruzi biotherapeutic worsened its murine infection by means of clinical evaluation. Considering other studies using biotherapeutic of T. cruzi, suggests the possibility for an efficacy of different dynamizations regarding oscillatory potency-effect-curves.","PeriodicalId":16091,"journal":{"name":"Journal of Experimental and Integrative Medicine","volume":"14 1","pages":"100-104"},"PeriodicalIF":0.0,"publicationDate":"2015-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89427338","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2015-01-01DOI: 10.5455/JEIM.191115.OR.139
S. Usman, G. Agboola, N. O. Akintayo-Usman, I. Isola, Florence Chinwe Umeozulu, P. Osho, Mattew T. Oluwole
Objectives: HIV/AIDS is a rapidly growing epidemic in sub-Saharan Africa. HIV is a retrovirus that primarily infects components of the human immune system such as CD4+ T-cells, macrophages and dendritic cells. This study is therefore designed to evaluate the CD4+ T-cell count and liver enzymes of adult HIV seropositive subjects receiving Highly Active Antiretroviral Therapy (HAART) and those yet to be started on HAART as well as HIV seronegative control subjects. Methods: Serum levels of CD4+ counts of subjects were determined using flow cytometry while their serum alanine aminotransferase (ALT), aspartate aminotransferase (AST) and alkaline phosphatase (ALP) were determined using enzymatic spectrophotometric method. Results: Serum ALT was found to be significantly increased in the HAART group as compared with those of the two other groups. In addition, serum ALP was significantly increased in the HAART group as compared with the control subjects, while serum AST was higher in the HAART group as compared with the HAART naive group. Conclusion: HAART is associated with hepatotoxicity, thus, it is recommended that liver enzymes should be measured before and periodically after antiretroviral therapy is initiated and/or when HAART regimen is switched, as this will serve a good index for disease monitoring and/or progression.
{"title":"Patterns of CD4+ T-lymphocytes and liver enzymes in adult HIV seropositive cases -","authors":"S. Usman, G. Agboola, N. O. Akintayo-Usman, I. Isola, Florence Chinwe Umeozulu, P. Osho, Mattew T. Oluwole","doi":"10.5455/JEIM.191115.OR.139","DOIUrl":"https://doi.org/10.5455/JEIM.191115.OR.139","url":null,"abstract":"Objectives: HIV/AIDS is a rapidly growing epidemic in sub-Saharan Africa. HIV is a retrovirus that primarily infects components of the human immune system such as CD4+ T-cells, macrophages and dendritic cells. This study is therefore designed to evaluate the CD4+ T-cell count and liver enzymes of adult HIV seropositive subjects receiving Highly Active Antiretroviral Therapy (HAART) and those yet to be started on HAART as well as HIV seronegative control subjects. Methods: Serum levels of CD4+ counts of subjects were determined using flow cytometry while their serum alanine aminotransferase (ALT), aspartate aminotransferase (AST) and alkaline phosphatase (ALP) were determined using enzymatic spectrophotometric method. Results: Serum ALT was found to be significantly increased in the HAART group as compared with those of the two other groups. In addition, serum ALP was significantly increased in the HAART group as compared with the control subjects, while serum AST was higher in the HAART group as compared with the HAART naive group. Conclusion: HAART is associated with hepatotoxicity, thus, it is recommended that liver enzymes should be measured before and periodically after antiretroviral therapy is initiated and/or when HAART regimen is switched, as this will serve a good index for disease monitoring and/or progression.","PeriodicalId":16091,"journal":{"name":"Journal of Experimental and Integrative Medicine","volume":"17 1","pages":"178-182"},"PeriodicalIF":0.0,"publicationDate":"2015-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89021887","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2015-01-01DOI: 10.5455/jeim.021215.or.143
Y. Gangaiah, Dinesh Rao, Stacy Merril, Sathees B. C. Chandra
Introduction: This was a prospective, randomized, placebo controlled, double blind study with the aim to determine if pre-treatment with ephedrine hydrochloride in adult patients during rapid tracheal intubation using propofol and rocuronium bromide improved intubating conditions without affecting hemodynamic parameters. Methods: One hundred adult patients, aged between 20 and 40 years, scheduled for various elective surgeries under general anesthesia were selected on the basis of a simple random sampling method. The study population was randomly divided using simple sealed envelope method into two groups, with 50 patients in each group: group EPR (ephedrine-propofol-rocuronium) and group SPR (saline-propofol-rocuronium). Patients in EPR group received ephedrine 70 µg/kg diluted to 5 ml with normal saline and the patients in SPR group received 5 ml of normal saline at the time of pre-oxygenation 3 min prior to laryngoscopy and intubation. Intubating conditions and hemodynamic parameters were assessed. Results: Pre-treatment with ephedrine hydrochloride prior to induction with propofol provided enhanced intubating conditions compared to propofol alone during rapid tracheal intubation 60 seconds after rocuronium bromide injection. However, it did not produce significant elevations of systolic and diastolic blood pressure as well as mean arterial pressure from the baseline. Pre-treatment in the EPR group resulted in an increase in the heart rate compared to the SPR group 1 min post intubation. Conclusion: The findings of the present study display that pre-treatment with ephedrine prior to propofol administration produces better intubating conditions compared to propofol alone during rapid tracheal intubation with rocuronium without significant hemodynamic changes. However, pre-treatment produced mild tachycardia, which was advantageous. It did not have any effect on the duration of laryngoscopy and the time taken for intubation.
{"title":"Hemodynamic changes during tracheal intubation using propofol and rocuronium after pre-treatment with ephedrine in adult patients -","authors":"Y. Gangaiah, Dinesh Rao, Stacy Merril, Sathees B. C. Chandra","doi":"10.5455/jeim.021215.or.143","DOIUrl":"https://doi.org/10.5455/jeim.021215.or.143","url":null,"abstract":"Introduction: This was a prospective, randomized, placebo controlled, double blind study with the aim to determine if pre-treatment with ephedrine hydrochloride in adult patients during rapid tracheal intubation using propofol and rocuronium bromide improved intubating conditions without affecting hemodynamic parameters. Methods: One hundred adult patients, aged between 20 and 40 years, scheduled for various elective surgeries under general anesthesia were selected on the basis of a simple random sampling method. The study population was randomly divided using simple sealed envelope method into two groups, with 50 patients in each group: group EPR (ephedrine-propofol-rocuronium) and group SPR (saline-propofol-rocuronium). Patients in EPR group received ephedrine 70 µg/kg diluted to 5 ml with normal saline and the patients in SPR group received 5 ml of normal saline at the time of pre-oxygenation 3 min prior to laryngoscopy and intubation. Intubating conditions and hemodynamic parameters were assessed. Results: Pre-treatment with ephedrine hydrochloride prior to induction with propofol provided enhanced intubating conditions compared to propofol alone during rapid tracheal intubation 60 seconds after rocuronium bromide injection. However, it did not produce significant elevations of systolic and diastolic blood pressure as well as mean arterial pressure from the baseline. Pre-treatment in the EPR group resulted in an increase in the heart rate compared to the SPR group 1 min post intubation. Conclusion: The findings of the present study display that pre-treatment with ephedrine prior to propofol administration produces better intubating conditions compared to propofol alone during rapid tracheal intubation with rocuronium without significant hemodynamic changes. However, pre-treatment produced mild tachycardia, which was advantageous. It did not have any effect on the duration of laryngoscopy and the time taken for intubation.","PeriodicalId":16091,"journal":{"name":"Journal of Experimental and Integrative Medicine","volume":"15 1","pages":"193-199"},"PeriodicalIF":0.0,"publicationDate":"2015-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87765406","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2015-01-01DOI: 10.5455/JEIM.080415.OR.125
U. Ukoha, Kosisochukwu E Umeasalugo, U. Dimkpa, G. Ndukwe, A. Anyabolu, Love E. Emefo
{"title":"Effects of zidovudine on hemostatic and hematologic parameters in adult rats","authors":"U. Ukoha, Kosisochukwu E Umeasalugo, U. Dimkpa, G. Ndukwe, A. Anyabolu, Love E. Emefo","doi":"10.5455/JEIM.080415.OR.125","DOIUrl":"https://doi.org/10.5455/JEIM.080415.OR.125","url":null,"abstract":"","PeriodicalId":16091,"journal":{"name":"Journal of Experimental and Integrative Medicine","volume":"40 1","pages":"85-88"},"PeriodicalIF":0.0,"publicationDate":"2015-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86615110","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2015-01-01DOI: 10.5455/jeim.120515.cr.131
O. Adewale, N. Orhue
Objectives: The burden of renal diseases is growing every day, and there is need to speed up research toward realizing the goal of a safe, cheap, and readily available remedy against this scourge. The objective of the study was to investigate the protective effect of aqueous extract of the fruit of Xylopia aethiopica against carbon tetrachloride (CCl4 )-induced kidney damage in rats. Materials and Methods: Thirty-six rats divided into 6 groups were used for the investigation. Group I served as control, Groups II, III, and IV were pre-treated for 21 days with aqueous extract of the fruit of X. aethiopica at doses of 250, 500, and 1000 mg/kg body weight, respectively, prior to a single intraperitoneal administration of CCl4 . Animals in groups V received only the fruit extract at a dose of 1000 mg/kg body weight while animals in group VI were given only CCl4 . Animals were sacrificed 24 h after the administration of CCl4 . The kidney function tests were performed in addition to histopathological evaluations. Results: Significant increase in the plasma levels of creatinine and urea, and a significant decrease in plasma total protein and albumin following CCl4 administration were detected. CCl4 caused a significant elevation in malondialdehyde and a decrease in the activities of the antioxidant enzymes in the kidney. These deleterious effects were significantly prevented by pre-treatment with the X. aethiopica extract. Conclusion: These findings suggest that X. aethiopica protection of the kidney against CCl4 -induced damage may be connected with the rich antioxidant potential of the plant.
{"title":"Protective effect of aqueous extract of Xylopia aethiopica fruits on carbon tetrachloride-induced nephrotoxicity in rats","authors":"O. Adewale, N. Orhue","doi":"10.5455/jeim.120515.cr.131","DOIUrl":"https://doi.org/10.5455/jeim.120515.cr.131","url":null,"abstract":"Objectives: The burden of renal diseases is growing every day, and there is need to speed up research toward realizing the goal of a safe, cheap, and readily available remedy against this scourge. The objective of the study was to investigate the protective effect of aqueous extract of the fruit of Xylopia aethiopica against carbon tetrachloride (CCl4 )-induced kidney damage in rats. Materials and Methods: Thirty-six rats divided into 6 groups were used for the investigation. Group I served as control, Groups II, III, and IV were pre-treated for 21 days with aqueous extract of the fruit of X. aethiopica at doses of 250, 500, and 1000 mg/kg body weight, respectively, prior to a single intraperitoneal administration of CCl4 . Animals in groups V received only the fruit extract at a dose of 1000 mg/kg body weight while animals in group VI were given only CCl4 . Animals were sacrificed 24 h after the administration of CCl4 . The kidney function tests were performed in addition to histopathological evaluations. Results: Significant increase in the plasma levels of creatinine and urea, and a significant decrease in plasma total protein and albumin following CCl4 administration were detected. CCl4 caused a significant elevation in malondialdehyde and a decrease in the activities of the antioxidant enzymes in the kidney. These deleterious effects were significantly prevented by pre-treatment with the X. aethiopica extract. Conclusion: These findings suggest that X. aethiopica protection of the kidney against CCl4 -induced damage may be connected with the rich antioxidant potential of the plant.","PeriodicalId":16091,"journal":{"name":"Journal of Experimental and Integrative Medicine","volume":"92 1","pages":"105-109"},"PeriodicalIF":0.0,"publicationDate":"2015-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77853677","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2015-01-01DOI: 10.5455/JEIM.181115.BR.026
N. Timchenko, M. Evstigneev, V. A. Rubakina
Objective: Hemoglobin (Hb) solutions are used in clinical practice and efforts are underway to study methods and techniques of their storage. As Hb solutions are stored, oxygen-intolerant methemoglobin (metHb) is formed. Increased metHb content in Hb solutions adversely affects the potential for their use. It is of particular interest for neonatology as increased metHb content is observed in healthy neonates. To simulate the processes taking place in metHb-containing red blood cells we researched the adult (A) and fetal (F) metHb interaction with liposomes. Methods: We obtained metHb А and F by oxidation of Hb А and F, respectively, with potassium hexacyanoferrate(ІІІ) excess and subsequent dialysis. Hb A was isolated from donors’ and Hb F from umbilical blood. Egg phosphatidylcholine and cardiolipin from bovine heart were used. Liposomes were formed in the 4:1 phosphatidylcholine-cardiolipin weight ratio. Protein-lipid complexes were formed at 20�С. The kinetics of metHb A and F interaction with liposomes was studied by recording the changes of optical density of protein-lipid complexes in the Soret band maximum. Results: The kinetics of metHb A and F interaction with liposomes showed that a decrease of the protein optical density in the Soret band for metHb F was more apparent than for metHb A. Conclusions: As far as metHb induces lipid peroxidation in model membranes, it is fair to assume that this process is more intensive in case of metHb F-liposomes interaction than in case of metHb A-liposomes interaction. Probably, metHb F is less resistant to hydroperoxides than metHb A.
{"title":"Adult and fetal methemoglobin interaction with liposomes","authors":"N. Timchenko, M. Evstigneev, V. A. Rubakina","doi":"10.5455/JEIM.181115.BR.026","DOIUrl":"https://doi.org/10.5455/JEIM.181115.BR.026","url":null,"abstract":"Objective: Hemoglobin (Hb) solutions are used in clinical practice and efforts are underway to study methods and techniques of their storage. As Hb solutions are stored, oxygen-intolerant methemoglobin (metHb) is formed. Increased metHb content in Hb solutions adversely affects the potential for their use. It is of particular interest for neonatology as increased metHb content is observed in healthy neonates. To simulate the processes taking place in metHb-containing red blood cells we researched the adult (A) and fetal (F) metHb interaction with liposomes. Methods: We obtained metHb А and F by oxidation of Hb А and F, respectively, with potassium hexacyanoferrate(ІІІ) excess and subsequent dialysis. Hb A was isolated from donors’ and Hb F from umbilical blood. Egg phosphatidylcholine and cardiolipin from bovine heart were used. Liposomes were formed in the 4:1 phosphatidylcholine-cardiolipin weight ratio. Protein-lipid complexes were formed at 20�С. The kinetics of metHb A and F interaction with liposomes was studied by recording the changes of optical density of protein-lipid complexes in the Soret band maximum. Results: The kinetics of metHb A and F interaction with liposomes showed that a decrease of the protein optical density in the Soret band for metHb F was more apparent than for metHb A. Conclusions: As far as metHb induces lipid peroxidation in model membranes, it is fair to assume that this process is more intensive in case of metHb F-liposomes interaction than in case of metHb A-liposomes interaction. Probably, metHb F is less resistant to hydroperoxides than metHb A.","PeriodicalId":16091,"journal":{"name":"Journal of Experimental and Integrative Medicine","volume":"49 1","pages":"169-171"},"PeriodicalIF":0.0,"publicationDate":"2015-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82043459","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2015-01-01DOI: 10.5455/jeim.160715.or.134
A. Zanasi, P. D. Nuntiis, M. Mazzolini, C. Ciantelli, M. Alemanni, Carla Maria Sofia Ierna, M. Mastroroberto, E. Nardi, A. Morselli-Labate
Objective: During the last decades, an increase in the prevalence of asthma and other allergic diseases has been recorded, together with modifications in the living environment and consequent changes in the quality of indoor air. Indoor environment is favorable to the proliferation of allergens such as: house dust mites, fungal spores and cockroaches. The primary action to be undertaken for an effective eradication of infectious agents constitutes in modifying the house environmental conditions, which make it favorable to infestations. Ozone can play a sanitize role, but at the same time it can cause inflammation, especially in the lung. The aim of this study was to verify the role and safety of ozone in the sanitation of the bedroom of a subject suffering from asthma. Methods: A daily ozone treatment was carried during a 14-day time period in the bedroom of an asthmatic patient. Aerobiological sampling in indoor air, microbiological sampling and detection of ATP bioluminescence on the surface were performed before and after treatment at the first day, as well as after treatment at the 7th and 14th day of the study. An aerobiological measurement was also performed outdoor of the patient’s bedroom only for the first day. Results: Our analysis confirms that low ozone levels induced a marked reduction of indoor air microbiological pollution without adverse effects on lung functionality of the asthmatic patient we considered. Conclusion: Our observations warrant further investigation on the role that ozone-based sterilization might have in controlling asthmatic symptoms.
{"title":"Can an ozone system generator reduce indoor triggers in asthmatic patient","authors":"A. Zanasi, P. D. Nuntiis, M. Mazzolini, C. Ciantelli, M. Alemanni, Carla Maria Sofia Ierna, M. Mastroroberto, E. Nardi, A. Morselli-Labate","doi":"10.5455/jeim.160715.or.134","DOIUrl":"https://doi.org/10.5455/jeim.160715.or.134","url":null,"abstract":"Objective: During the last decades, an increase in the prevalence of asthma and other allergic diseases has been recorded, together with modifications in the living environment and consequent changes in the quality of indoor air. Indoor environment is favorable to the proliferation of allergens such as: house dust mites, fungal spores and cockroaches. The primary action to be undertaken for an effective eradication of infectious agents constitutes in modifying the house environmental conditions, which make it favorable to infestations. Ozone can play a sanitize role, but at the same time it can cause inflammation, especially in the lung. The aim of this study was to verify the role and safety of ozone in the sanitation of the bedroom of a subject suffering from asthma. Methods: A daily ozone treatment was carried during a 14-day time period in the bedroom of an asthmatic patient. Aerobiological sampling in indoor air, microbiological sampling and detection of ATP bioluminescence on the surface were performed before and after treatment at the first day, as well as after treatment at the 7th and 14th day of the study. An aerobiological measurement was also performed outdoor of the patient’s bedroom only for the first day. Results: Our analysis confirms that low ozone levels induced a marked reduction of indoor air microbiological pollution without adverse effects on lung functionality of the asthmatic patient we considered. Conclusion: Our observations warrant further investigation on the role that ozone-based sterilization might have in controlling asthmatic symptoms.","PeriodicalId":16091,"journal":{"name":"Journal of Experimental and Integrative Medicine","volume":"107 1","pages":"128-136"},"PeriodicalIF":0.0,"publicationDate":"2015-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79155060","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2015-01-01DOI: 10.5455/JEIM.160615.RW.012
A. Mohammed, Dileep Kumar, S. Rizvi
{"title":"Emergence of traditional antidiabetic treatments as starting points for development of modern medicine","authors":"A. Mohammed, Dileep Kumar, S. Rizvi","doi":"10.5455/JEIM.160615.RW.012","DOIUrl":"https://doi.org/10.5455/JEIM.160615.RW.012","url":null,"abstract":"","PeriodicalId":16091,"journal":{"name":"Journal of Experimental and Integrative Medicine","volume":"19 1","pages":"121-127"},"PeriodicalIF":0.0,"publicationDate":"2015-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84163586","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2015-01-01DOI: 10.5455/JEIM.191115.OR.138
Mahmoud Helmy Belal, Mohamed Bahaa Kheidr
Objective: Ulcerative lesions on lower lip are common. Aggressive forms lead to lip distortion and affect function and esthetic. Suggested treatment strategies are still facing controversy. This study aimed to evaluate the potential therapeutic efficacy of a combination of topical hyaluronic acid (HA) and systemic selenium plus vitamin A, C and E (ACE). Methods: Nine patients affected with symptomatic lesions on lower lip of erosive-ulcerative areas with moderate severity were enrolled. Age range: 47~67 years. They were diagnosed clinically and histopathologically by incisional biopsy. A topical HA gel was applied undiluted with a cotton bud four times daily. Selenium-ACE was prescribed systemically once daily. Treatments were applied for a 6-week period and lesions’ areas were followed for six months for any recurrence. Pain and severity of lesions were scored. Results: All patients had sun exposure, six were heavy smokers whereas two had sometimes alcohol consumption. In general, six patients were diagnosed as benign lesions that healed uneventfully with complete resolution. Four of them were inflammatory lesions, whereas two were oral lichen planus. On contrary, three patients were malignant (SCC), did not heal completely and referred to surgical resection with safety margin. No allergy was reported to used medications. Furthermore, pain sensation and lesion size were significantly improved within benign patients for the whole intervals except only 2 vs 4 weeks that showed no improvement. On contrary, no significant improvements occurred within malignant patients, neither for lesion healing nor pain alleviation, except only 2 vs 6 weeks that showed alleviation of symptoms. Conclusion: Topical HA gel combined with selenium-ACE may be a useful additional treatment option for erosive ulcerative lesions of the lower lip.
{"title":"Erosive-ulcerative lesions on the lower lip with malignant potential: efficacy of incisional biopsy followed by topical hyaluronic acid and systemic selenium-vitamin combination","authors":"Mahmoud Helmy Belal, Mohamed Bahaa Kheidr","doi":"10.5455/JEIM.191115.OR.138","DOIUrl":"https://doi.org/10.5455/JEIM.191115.OR.138","url":null,"abstract":"Objective: Ulcerative lesions on lower lip are common. Aggressive forms lead to lip distortion and affect function and esthetic. Suggested treatment strategies are still facing controversy. This study aimed to evaluate the potential therapeutic efficacy of a combination of topical hyaluronic acid (HA) and systemic selenium plus vitamin A, C and E (ACE). Methods: Nine patients affected with symptomatic lesions on lower lip of erosive-ulcerative areas with moderate severity were enrolled. Age range: 47~67 years. They were diagnosed clinically and histopathologically by incisional biopsy. A topical HA gel was applied undiluted with a cotton bud four times daily. Selenium-ACE was prescribed systemically once daily. Treatments were applied for a 6-week period and lesions’ areas were followed for six months for any recurrence. Pain and severity of lesions were scored. Results: All patients had sun exposure, six were heavy smokers whereas two had sometimes alcohol consumption. In general, six patients were diagnosed as benign lesions that healed uneventfully with complete resolution. Four of them were inflammatory lesions, whereas two were oral lichen planus. On contrary, three patients were malignant (SCC), did not heal completely and referred to surgical resection with safety margin. No allergy was reported to used medications. Furthermore, pain sensation and lesion size were significantly improved within benign patients for the whole intervals except only 2 vs 4 weeks that showed no improvement. On contrary, no significant improvements occurred within malignant patients, neither for lesion healing nor pain alleviation, except only 2 vs 6 weeks that showed alleviation of symptoms. Conclusion: Topical HA gel combined with selenium-ACE may be a useful additional treatment option for erosive ulcerative lesions of the lower lip.","PeriodicalId":16091,"journal":{"name":"Journal of Experimental and Integrative Medicine","volume":"2010 1","pages":"209-214"},"PeriodicalIF":0.0,"publicationDate":"2015-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82592727","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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