Pub Date : 2014-01-01DOI: 10.5455/JEIM.101113.CR.004
Amir-ur Rehman, J. Berry, M. A. Siddiqui
Post stroke rehabilitation has been consistently reported in physiotherapy as being difficult to manage and limiting to rehabilitation outcome. It is reported that the first few months post stroke are crucial for rehabilitation to reach a maximum potential. However, after this period further recovery is unlikely. Specific, measurable, achievable, realistic, and time-framed (SMART) goals are used for goal setting for rehabilitation. They can provide coordination between a multidisciplinary team to develop a plan for the individual needs of patients. This case study explored the use of SMART goals, which are often not used in a rehabilitation setting due to being time-consuming and difficult to create. This case report provides the first evidence that SMART goals may serve as a powerful tool for rehabilitation following stroke. The case study illustrates the clinical role of physiotherapists in the management of a patient with stroke.
{"title":"Post stroke rehabilitation based on SMART goals: a case study","authors":"Amir-ur Rehman, J. Berry, M. A. Siddiqui","doi":"10.5455/JEIM.101113.CR.004","DOIUrl":"https://doi.org/10.5455/JEIM.101113.CR.004","url":null,"abstract":"Post stroke rehabilitation has been consistently reported in physiotherapy as being difficult to manage and limiting to rehabilitation outcome. It is reported that the first few months post stroke are crucial for rehabilitation to reach a maximum potential. However, after this period further recovery is unlikely. Specific, measurable, achievable, realistic, and time-framed (SMART) goals are used for goal setting for rehabilitation. They can provide coordination between a multidisciplinary team to develop a plan for the individual needs of patients. This case study explored the use of SMART goals, which are often not used in a rehabilitation setting due to being time-consuming and difficult to create. This case report provides the first evidence that SMART goals may serve as a powerful tool for rehabilitation following stroke. The case study illustrates the clinical role of physiotherapists in the management of a patient with stroke.","PeriodicalId":16091,"journal":{"name":"Journal of Experimental and Integrative Medicine","volume":"67 1","pages":"71-73"},"PeriodicalIF":0.0,"publicationDate":"2014-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80260672","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2014-01-01DOI: 10.5455/JEIM.150514.OR.105
S. Yalçın, Ş. Demirbaş, O. Onguru
Objective: IL-18 has been shown to play a protective role in the host defense towards different sources of infection or to have a pathological role in hepatitis B (HBV). Methods: For the purposes of this study, we analyzed IL-18 single-nucleotide polymorphisms (SNPs) in hepatitis B virus carrier, chronic hepatitis, cirrhosis, and non-infected individuals. Presence of the IL-18 genotyping was performed using the PCR amplification and DNA sequencing. Results: Significant associations were detected between the IL-18 rs187238 (G/C) polymorphism and infected patients. Conclusion: Based on our data, there were no significant associations between the IL-18 rs61667799(G/T), rs5744227(C/G), rs5744228(A/G) polymorphism in Turkish HBV infected and non-infected individuals with HBV.
{"title":"Investigation of interleukin-18 polymorphism in hepatit B virus carriers, chronic hepatitis and cirrhosis patients -","authors":"S. Yalçın, Ş. Demirbaş, O. Onguru","doi":"10.5455/JEIM.150514.OR.105","DOIUrl":"https://doi.org/10.5455/JEIM.150514.OR.105","url":null,"abstract":"Objective: IL-18 has been shown to play a protective role in the host defense towards different sources of infection or to have a pathological role in hepatitis B (HBV). Methods: For the purposes of this study, we analyzed IL-18 single-nucleotide polymorphisms (SNPs) in hepatitis B virus carrier, chronic hepatitis, cirrhosis, and non-infected individuals. Presence of the IL-18 genotyping was performed using the PCR amplification and DNA sequencing. Results: Significant associations were detected between the IL-18 rs187238 (G/C) polymorphism and infected patients. Conclusion: Based on our data, there were no significant associations between the IL-18 rs61667799(G/T), rs5744227(C/G), rs5744228(A/G) polymorphism in Turkish HBV infected and non-infected individuals with HBV.","PeriodicalId":16091,"journal":{"name":"Journal of Experimental and Integrative Medicine","volume":"204 1","pages":"175-179"},"PeriodicalIF":0.0,"publicationDate":"2014-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72950189","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2014-01-01DOI: 10.5455/JEIM.271014.OR.114
S. Onoja, Celestine O. Ukwueze, M. I. Ezeja, N. Udeh
Objective: Bridelia micrantha is among the commonly used herbs in the traditional management of disease conditions. This study aimed to evaluate the antinociceptive and antioxidant effects of the hydromethanolic extract of B.micrantha stem bark. Methods: The acute oral toxicity of the extract was evaluated using up and down method. The antinociceptive effects of B.micrantha extract at the doses of 50, 100 and 200 mg/kg were investigated using acetic acid induced writhing reflex and tail flick method. The effect of B.micrantha on thiopentone induced narcosis was also investigated. The antioxidant effect of B.micrantha was investigated using 2,2-diphenyl-1-picrylhydrazyl (DPPH) photometric assay. Results: The extract was well tolerated at the high dose (2000 mg/kg) used for the acute toxicity study. The extract produced a significant dose-dependent decrease in the mean number of abdominal constriction in the acetic acid induced writhing reflex when compared to the negative control. Both the extract (200 mg/kg) and paracetamol (400 mg/kg) produced 61.85 and 73.08% inhibition of writhing reflex, respectively. B.micrantha and pentazocine (3 mg/kg) caused significant increase in the pain reaction time in treated rats when compared to the negative control group in tail flick method. The pretreatment of the rats with B.micrantha at different doses increased the normal sleeping time of thiopentone from 69.33 ± 7.31 min to an average of 105.33 ± 11.88 min. The extract also produced concentration-dependent increase in percentage antioxidant activity in DPPH photometric assay. Conclusion: This study demonstrates that Bridelia micrantha possess potent antinociceptive and antioxidant activities which validate its use in folkloric medicine for this purpose.
{"title":"Antinociceptive and antioxidant effects of hydromethanolic extract of Bridelia micrantha stem bark","authors":"S. Onoja, Celestine O. Ukwueze, M. I. Ezeja, N. Udeh","doi":"10.5455/JEIM.271014.OR.114","DOIUrl":"https://doi.org/10.5455/JEIM.271014.OR.114","url":null,"abstract":"Objective: Bridelia micrantha is among the commonly used herbs in the traditional management of disease conditions. This study aimed to evaluate the antinociceptive and antioxidant effects of the hydromethanolic extract of B.micrantha stem bark. Methods: The acute oral toxicity of the extract was evaluated using up and down method. The antinociceptive effects of B.micrantha extract at the doses of 50, 100 and 200 mg/kg were investigated using acetic acid induced writhing reflex and tail flick method. The effect of B.micrantha on thiopentone induced narcosis was also investigated. The antioxidant effect of B.micrantha was investigated using 2,2-diphenyl-1-picrylhydrazyl (DPPH) photometric assay. Results: The extract was well tolerated at the high dose (2000 mg/kg) used for the acute toxicity study. The extract produced a significant dose-dependent decrease in the mean number of abdominal constriction in the acetic acid induced writhing reflex when compared to the negative control. Both the extract (200 mg/kg) and paracetamol (400 mg/kg) produced 61.85 and 73.08% inhibition of writhing reflex, respectively. B.micrantha and pentazocine (3 mg/kg) caused significant increase in the pain reaction time in treated rats when compared to the negative control group in tail flick method. The pretreatment of the rats with B.micrantha at different doses increased the normal sleeping time of thiopentone from 69.33 ± 7.31 min to an average of 105.33 ± 11.88 min. The extract also produced concentration-dependent increase in percentage antioxidant activity in DPPH photometric assay. Conclusion: This study demonstrates that Bridelia micrantha possess potent antinociceptive and antioxidant activities which validate its use in folkloric medicine for this purpose.","PeriodicalId":16091,"journal":{"name":"Journal of Experimental and Integrative Medicine","volume":"1 1","pages":"273-277"},"PeriodicalIF":0.0,"publicationDate":"2014-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78709717","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2014-01-01DOI: 10.5455/JEIM.120414.BR.021
Yumi Abimulyani, N. Nurdiana, S. C. W. Baktiyani
Objective: This study aimed to investigate whether an ethanolic extract of Syzygium cumini is able to decrease lipid peroxidation and matrix metalloproteinase (MMP)-2 expression in endothelial cells exposed to plasma from preeclamptic patients. Methods: Endothelial cells were obtained from human umbilical vascular endothelial cells. At confluency, endothelial cells were divided into five groups, which included control (untreated), endothelial cells exposed to 2% plasma from preeclamptic patients (PP), endothelial cells exposed to PP in the presence of ethanolic extract of S.cumini (PP+SC) at the doses of 100, 200 and 400 ppm. Analysis of malondialdehyde (MDA) level as a marker of lipid peroxidation was done colorimetrically. Analysis of MMP-2 level was done by enzyme linked immunosorbent assay (ELISA) technically. Results: This increase in MDA was significantly attenuated by both the 200 and 400 ppm treatments of S.cumini extract. Plasma from PP significantly increased MMP-2 levels compared to untreated cells. This increase in MMP-2 was significantly attenuated by the 200 and 400 ppm doses of the extract. Conclusion: In conclusion, Syzygium cumini extract prohibits the increase in oxidative stress and MMP-2 in endothelial cells induced by plasma from preeclamptic patients. Therefore this may provide a natural therapy for attenuating the maternal endothelial dysfunction found in this disease.
{"title":"Syzygium cumini reduces oxidative stress and matrix metalloproteinase-2 level in endothelial cells induced by plasma from preeclamptic patients -","authors":"Yumi Abimulyani, N. Nurdiana, S. C. W. Baktiyani","doi":"10.5455/JEIM.120414.BR.021","DOIUrl":"https://doi.org/10.5455/JEIM.120414.BR.021","url":null,"abstract":"Objective: This study aimed to investigate whether an ethanolic extract of Syzygium cumini is able to decrease lipid peroxidation and matrix metalloproteinase (MMP)-2 expression in endothelial cells exposed to plasma from preeclamptic patients. Methods: Endothelial cells were obtained from human umbilical vascular endothelial cells. At confluency, endothelial cells were divided into five groups, which included control (untreated), endothelial cells exposed to 2% plasma from preeclamptic patients (PP), endothelial cells exposed to PP in the presence of ethanolic extract of S.cumini (PP+SC) at the doses of 100, 200 and 400 ppm. Analysis of malondialdehyde (MDA) level as a marker of lipid peroxidation was done colorimetrically. Analysis of MMP-2 level was done by enzyme linked immunosorbent assay (ELISA) technically. Results: This increase in MDA was significantly attenuated by both the 200 and 400 ppm treatments of S.cumini extract. Plasma from PP significantly increased MMP-2 levels compared to untreated cells. This increase in MMP-2 was significantly attenuated by the 200 and 400 ppm doses of the extract. Conclusion: In conclusion, Syzygium cumini extract prohibits the increase in oxidative stress and MMP-2 in endothelial cells induced by plasma from preeclamptic patients. Therefore this may provide a natural therapy for attenuating the maternal endothelial dysfunction found in this disease.","PeriodicalId":16091,"journal":{"name":"Journal of Experimental and Integrative Medicine","volume":"10 1","pages":"89-92"},"PeriodicalIF":0.0,"publicationDate":"2014-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90530761","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2014-01-01DOI: 10.5455/JEIM.200414.OR.102
S. Sutrisno, Dwina Mastryagung, Rahayu Khairiah, Feva Tridiyawati, Nyoman Artina, D. Hidayati, Noorhamdani Noorhamdani, S. Santoso
Objectives: This study aimed to investigate whether genistein is able to inhibit cell proliferation and expression of estrogenic receptors, and to induce apoptosis in endometriosis cells. Methods: Confluent primary endometriosis cells were divided into seven groups, including control group and genistein treatment group at doses of 5, 10, 20, 30, 40 and 50 µmol/l. The times of incubation were 6, 24 and 48 hours. Four replications are applied in each group. Expression of estrogen receptor-α and estrogen receptor-β and level of proliferation cells in cells were measured using flow cytometry. Level of apoptosis cells and Bax were measured by enzyme-linked immunosorbent technique. Results: In 6 hours of treatment, the expression of estrogen receptor-α and estrogen receptor-β significantly decreased at all doses of genistein compared with control group. The expression of estrogen receptor-α was significantly reduced in 5 until 40 µM of 24 hours genistein treatment groups in comparison to control group, but estrogen receptor-β was not significantly different. In 48 hours of treatment, the expression of estrogen receptor-α and estrogen receptor-β were significantly lower in genistein-treated groups at doses of 40 µM and 20-50 µM, respectively, compared to control group. With 6 hours of treatment, the level of KI-67 and apoptosis significantly decreased in 50 µM genistein treatment group compared to control group. The expression of KI-67 significantly reduced at all doses of 24 hours genistein treatment compared to control group. In 48 hours of treatment, the level of KI-67 and apoptosis were significantly lower in 40 µM genistein treatment group compared to control group. The level of apoptosis was significantly higher in genistein treatment group compared to control group in 24 hours 50 µM genistein treatment group. Level of Bax significantly increased in genistein treatment at the doses from 10 until 50 µM compared to that of control group. Conclusion: Genistein inhibits endometrial cell proliferation and estrogen receptor expression, and induces apoptosis in endometriosis cell culture.
{"title":"The effects of genistein on estrogen receptor expression, cell proliferation, and apoptosis in endometriosis cell culture -","authors":"S. Sutrisno, Dwina Mastryagung, Rahayu Khairiah, Feva Tridiyawati, Nyoman Artina, D. Hidayati, Noorhamdani Noorhamdani, S. Santoso","doi":"10.5455/JEIM.200414.OR.102","DOIUrl":"https://doi.org/10.5455/JEIM.200414.OR.102","url":null,"abstract":"Objectives: This study aimed to investigate whether genistein is able to inhibit cell proliferation and expression of estrogenic receptors, and to induce apoptosis in endometriosis cells. Methods: Confluent primary endometriosis cells were divided into seven groups, including control group and genistein treatment group at doses of 5, 10, 20, 30, 40 and 50 µmol/l. The times of incubation were 6, 24 and 48 hours. Four replications are applied in each group. Expression of estrogen receptor-α and estrogen receptor-β and level of proliferation cells in cells were measured using flow cytometry. Level of apoptosis cells and Bax were measured by enzyme-linked immunosorbent technique. Results: In 6 hours of treatment, the expression of estrogen receptor-α and estrogen receptor-β significantly decreased at all doses of genistein compared with control group. The expression of estrogen receptor-α was significantly reduced in 5 until 40 µM of 24 hours genistein treatment groups in comparison to control group, but estrogen receptor-β was not significantly different. In 48 hours of treatment, the expression of estrogen receptor-α and estrogen receptor-β were significantly lower in genistein-treated groups at doses of 40 µM and 20-50 µM, respectively, compared to control group. With 6 hours of treatment, the level of KI-67 and apoptosis significantly decreased in 50 µM genistein treatment group compared to control group. The expression of KI-67 significantly reduced at all doses of 24 hours genistein treatment compared to control group. In 48 hours of treatment, the level of KI-67 and apoptosis were significantly lower in 40 µM genistein treatment group compared to control group. The level of apoptosis was significantly higher in genistein treatment group compared to control group in 24 hours 50 µM genistein treatment group. Level of Bax significantly increased in genistein treatment at the doses from 10 until 50 µM compared to that of control group. Conclusion: Genistein inhibits endometrial cell proliferation and estrogen receptor expression, and induces apoptosis in endometriosis cell culture.","PeriodicalId":16091,"journal":{"name":"Journal of Experimental and Integrative Medicine","volume":"58 1","pages":"201-206"},"PeriodicalIF":0.0,"publicationDate":"2014-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84449820","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2014-01-01DOI: 10.5455/JEIM.060514.OR.103
Imroatul Azizah, Tria Wahyuningrum, K. Keman, S. Santoso, D. Hidayati
Objective: This study purposed to analyze whether Vigna unguiculata is able to increase endothelial cell number, endothelial nitric oxide synthase (eNOS) expression, and to inhibit atherosclerosis in ovariectomized rats. Methods: After acclimatization for one week, twenty four female virgin rats were divided into four groups (n = 6 for each) including ovariectomized (OVX) and OVX + Vigna unguiculata groups; three doses of V.unguiculata extract were applied: 0.5, 2.5 and 5 mg/kg body weight. The number of aortic endothelial cells was counted histologically. Endothelial NOS expression of aortic endothelial cells was analyzed by immunohistochemistry. Serum lipid profile analysis was performed via standard colorimetric methods. In addition, foam cells analysis in aorta was done using oil red O staining in frozen section tissue specimens. Results: The number of endothelial cells were found to be significantly higher in OVX plus 2.5 and 5 mg/kg V.unguiculata extract applied groups compared with OVX alone (control) group. The expression of aortic eNOS in the OVX + 5 mg/kg V.unguiculata applied group was significantly higher compared with control group. V.unguiculata administration at all doses significantly decreased serum levels of low density lipoprotein and triglyceride in comparison to the control animals; on the other hand, total cholesterol levels significantly reduced with administration of V.unguiculata extract at doses of 2.5 and 5 mg/kg, and high density lipoprotein levels increased at 5 mg/kg. Aortic foam cells were found significantly decreased at all doses of V.unguiculata extract. Conclusion: Vigna unguiculata seem to be a good alternative for increasing endotheliall cell number and eNOS expression in the ovariectomy model used. Besides, V.unguiculata also act as anti-atherosclerotic agent by normalizing serum lipid profiles.
{"title":"The effects of Vigna unguiculata on aortic endothelial cells, endothelial nitric oxide synthase expression, lipid profile, and atherosclerosis in ovariectomized rats","authors":"Imroatul Azizah, Tria Wahyuningrum, K. Keman, S. Santoso, D. Hidayati","doi":"10.5455/JEIM.060514.OR.103","DOIUrl":"https://doi.org/10.5455/JEIM.060514.OR.103","url":null,"abstract":"Objective: This study purposed to analyze whether Vigna unguiculata is able to increase endothelial cell number, endothelial nitric oxide synthase (eNOS) expression, and to inhibit atherosclerosis in ovariectomized rats. Methods: After acclimatization for one week, twenty four female virgin rats were divided into four groups (n = 6 for each) including ovariectomized (OVX) and OVX + Vigna unguiculata groups; three doses of V.unguiculata extract were applied: 0.5, 2.5 and 5 mg/kg body weight. The number of aortic endothelial cells was counted histologically. Endothelial NOS expression of aortic endothelial cells was analyzed by immunohistochemistry. Serum lipid profile analysis was performed via standard colorimetric methods. In addition, foam cells analysis in aorta was done using oil red O staining in frozen section tissue specimens. Results: The number of endothelial cells were found to be significantly higher in OVX plus 2.5 and 5 mg/kg V.unguiculata extract applied groups compared with OVX alone (control) group. The expression of aortic eNOS in the OVX + 5 mg/kg V.unguiculata applied group was significantly higher compared with control group. V.unguiculata administration at all doses significantly decreased serum levels of low density lipoprotein and triglyceride in comparison to the control animals; on the other hand, total cholesterol levels significantly reduced with administration of V.unguiculata extract at doses of 2.5 and 5 mg/kg, and high density lipoprotein levels increased at 5 mg/kg. Aortic foam cells were found significantly decreased at all doses of V.unguiculata extract. Conclusion: Vigna unguiculata seem to be a good alternative for increasing endotheliall cell number and eNOS expression in the ovariectomy model used. Besides, V.unguiculata also act as anti-atherosclerotic agent by normalizing serum lipid profiles.","PeriodicalId":16091,"journal":{"name":"Journal of Experimental and Integrative Medicine","volume":"5 1","pages":"207-211"},"PeriodicalIF":0.0,"publicationDate":"2014-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78920170","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2014-01-01DOI: 10.5455/JEIM.150514.OR.104
A. Saad, El-Hassan M. Mokhamer, M. Mohsen, G. Fadaly
Objective: Mounting clinical and experimental evidence has demonstrated that even advanced fibrosis and cirrhosis are reversible. Thus, there is a considerable imperative to develop antifibrotic strategies that are applicable to liver fibrosis. In the present study, the attenuation of carbon tetrachloride (CCl 4 )-induced hepatic fibrosis in rats through the co-treatment with antioxidant vitamins C and E or glycine alone was investigated. Materials and Methods: Sixty male Sprague-Dawley were divided randomly into control, CCl 4 , vitamin E+C, and glycine groups. Except for the control group, all rats in the other groups received orally 2 ml/kg CCl 4 dissolved in olive oil (1:1, v/v) twice a week; antioxidant vitamins were supplemented orally (p.o.) at a dose of 200 IU/kg/day vitamin E and 50 mg/kg/day vitamin C. Glycine dose was 0.6 g/Kg/day (p.o.). After 9 weeks, all rats were sacrificed for biochemical and histopathological investigations. Results: Serum levels of hepatic transaminases, alkaline phosphatase, gamma glutamyl transferase, and β-glucuronidase strikingly increased in CCl 4 group. As a biomarker of fibrinogenesis, hyaluronic acid was significantly elevated. These were associated with drastic significant decline in serum albumin concentration level, blood glutathione (GSH) content, GSH peroxidase, catalase and superoxide dismutase enzymatic activities, as well as a striking significant increase in the concentration of serum malondialdehyde. A significant increase in the mean serum concentration levels of tumor necrosis factor-a and transforming growth factor-b1 has been observed in CCl 4 -treated rats. Histopathological examination highlighted and confirmed the biochemical results. Conclusion: The results of this study confirmed the ameliorative effects of vitamin E plus C combination and glycine supplements against liver fibrosis in rats induced by CCl 4 .
{"title":"Attenuation of carbon tetrachloride-induced hepatic fibrosis by glycine, vitamin E and vitamin C -","authors":"A. Saad, El-Hassan M. Mokhamer, M. Mohsen, G. Fadaly","doi":"10.5455/JEIM.150514.OR.104","DOIUrl":"https://doi.org/10.5455/JEIM.150514.OR.104","url":null,"abstract":"Objective: Mounting clinical and experimental evidence has demonstrated that even advanced fibrosis and cirrhosis are reversible. Thus, there is a considerable imperative to develop antifibrotic strategies that are applicable to liver fibrosis. In the present study, the attenuation of carbon tetrachloride (CCl 4 )-induced hepatic fibrosis in rats through the co-treatment with antioxidant vitamins C and E or glycine alone was investigated. Materials and Methods: Sixty male Sprague-Dawley were divided randomly into control, CCl 4 , vitamin E+C, and glycine groups. Except for the control group, all rats in the other groups received orally 2 ml/kg CCl 4 dissolved in olive oil (1:1, v/v) twice a week; antioxidant vitamins were supplemented orally (p.o.) at a dose of 200 IU/kg/day vitamin E and 50 mg/kg/day vitamin C. Glycine dose was 0.6 g/Kg/day (p.o.). After 9 weeks, all rats were sacrificed for biochemical and histopathological investigations. Results: Serum levels of hepatic transaminases, alkaline phosphatase, gamma glutamyl transferase, and β-glucuronidase strikingly increased in CCl 4 group. As a biomarker of fibrinogenesis, hyaluronic acid was significantly elevated. These were associated with drastic significant decline in serum albumin concentration level, blood glutathione (GSH) content, GSH peroxidase, catalase and superoxide dismutase enzymatic activities, as well as a striking significant increase in the concentration of serum malondialdehyde. A significant increase in the mean serum concentration levels of tumor necrosis factor-a and transforming growth factor-b1 has been observed in CCl 4 -treated rats. Histopathological examination highlighted and confirmed the biochemical results. Conclusion: The results of this study confirmed the ameliorative effects of vitamin E plus C combination and glycine supplements against liver fibrosis in rats induced by CCl 4 .","PeriodicalId":16091,"journal":{"name":"Journal of Experimental and Integrative Medicine","volume":"14 1","pages":"180-186"},"PeriodicalIF":0.0,"publicationDate":"2014-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80703037","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2014-01-01DOI: 10.5455/JEIM.230114.OR.095
Daniel L. Medeiros, T. E. V. Lemos, Luiz H. Fagundes Junior, Tarciso B. Sampaio Montenegro, Paula R. S. Camara
Objective: The anti-inflammatory activity of copaiba oil was evaluated using a cerulein-induced acute pancreatitis model in mice. Methods: Mice were pretreated with Copaiba sp oleoresin before induction of pancreatitis. Pancreatitis was induced by intraperitoneal injection (five injections at hourly intervals) of cerulein solution and, 6 h later, pancreatic and lung damage were analyzed macroscopically for the severity of necrosis, and by protein plasma extravasation and plasma amylase activity (a biomarker of acute pancreatic damage); myeloperoxidase activity (MPO; a neutrophil marker) and thiobarbituric reactive species (TBARS; a lipid peroxidation index) were determined in the pancreas and lung. Results: A significant reduction in protein plasma extravasation of damaged pancreatic tissue was observed in mice that received copaiba oil. This effect was confirmed biochemically by reduction of protein plasma extravasation, and associated with reduced plasma amylase and MPO activity. Conclusion: These findings indicate the anti-inflammatory effect of copaiba oil on experimental acute pancreatitis induced by cerulein in mice.
{"title":"Effect of copaiba oil on acute pancreatitis in mice","authors":"Daniel L. Medeiros, T. E. V. Lemos, Luiz H. Fagundes Junior, Tarciso B. Sampaio Montenegro, Paula R. S. Camara","doi":"10.5455/JEIM.230114.OR.095","DOIUrl":"https://doi.org/10.5455/JEIM.230114.OR.095","url":null,"abstract":"Objective: The anti-inflammatory activity of copaiba oil was evaluated using a cerulein-induced acute pancreatitis model in mice. Methods: Mice were pretreated with Copaiba sp oleoresin before induction of pancreatitis. Pancreatitis was induced by intraperitoneal injection (five injections at hourly intervals) of cerulein solution and, 6 h later, pancreatic and lung damage were analyzed macroscopically for the severity of necrosis, and by protein plasma extravasation and plasma amylase activity (a biomarker of acute pancreatic damage); myeloperoxidase activity (MPO; a neutrophil marker) and thiobarbituric reactive species (TBARS; a lipid peroxidation index) were determined in the pancreas and lung. Results: A significant reduction in protein plasma extravasation of damaged pancreatic tissue was observed in mice that received copaiba oil. This effect was confirmed biochemically by reduction of protein plasma extravasation, and associated with reduced plasma amylase and MPO activity. Conclusion: These findings indicate the anti-inflammatory effect of copaiba oil on experimental acute pancreatitis induced by cerulein in mice.","PeriodicalId":16091,"journal":{"name":"Journal of Experimental and Integrative Medicine","volume":"11 1","pages":"107-114"},"PeriodicalIF":0.0,"publicationDate":"2014-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80198331","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2014-01-01DOI: 10.5455/JEIM.060514.RW.008
I. Kalniņa, E. Kirilova, G. Gorbenko, G. Kirilov, T. Zvagule, R. Linde, J. Reste
Albumin is the most generously represented protein in human blood plasma. Therefore it is important to follow and assess the transport function of albumin in clinic researches. Disturbances in structural/functional properties of albumin play an important role in the pathogenesis of various diseases and immune state in patients. Changes in albumin transformation can serve as a diagnostic and prognostic criterion in pathologies. ABM (3-aminobenzanthrone derivative developed at the Daugavpils University, Latvia) has been previously shown as a potential biomarker for determination of the immune state of patients with different pathologies. The aim of this study was to determine the several aspects of plasma albumin alterations in the group of Chernobyl clean-up workers in long term period in relation with humans having no professional contact with radioactivity. The following parameters were examined: (1) spectral characteristics of ABM in blood plasma; (2) ‘effective’ and total albumin (EA and TA) concentration in blood plasma; (3) quantitative parameters of albumin auto-fluorescence; (4) albumin binding site characteristics. Screening of the individuals with a period of 25-26 years after the work in Chernobyl revealed two groups of patients differing in structural and functional albumin properties; first on conformations of plasma albumin, and second characteristics of tryptophanyl region of the molecule. The revealed structural modifications of albumin are dependent on radiation-induced factors. Concomitant diseases such as diabetes mellitus or cardio-vascular diseases reinforce radiation-induced effects. In conclusion, ABM is a sensitive probe for albumin alterations and can be used to elucidate the changes in protein systems. Significant differences in albumin dynamics exist between control (donors) and groups of Chernobyl clean-up workers.
{"title":"Long term alterations of blood plasma albumin in Chernobyl clean-up workers","authors":"I. Kalniņa, E. Kirilova, G. Gorbenko, G. Kirilov, T. Zvagule, R. Linde, J. Reste","doi":"10.5455/JEIM.060514.RW.008","DOIUrl":"https://doi.org/10.5455/JEIM.060514.RW.008","url":null,"abstract":"Albumin is the most generously represented protein in human blood plasma. Therefore it is important to follow and assess the transport function of albumin in clinic researches. Disturbances in structural/functional properties of albumin play an important role in the pathogenesis of various diseases and immune state in patients. Changes in albumin transformation can serve as a diagnostic and prognostic criterion in pathologies. ABM (3-aminobenzanthrone derivative developed at the Daugavpils University, Latvia) has been previously shown as a potential biomarker for determination of the immune state of patients with different pathologies. The aim of this study was to determine the several aspects of plasma albumin alterations in the group of Chernobyl clean-up workers in long term period in relation with humans having no professional contact with radioactivity. The following parameters were examined: (1) spectral characteristics of ABM in blood plasma; (2) ‘effective’ and total albumin (EA and TA) concentration in blood plasma; (3) quantitative parameters of albumin auto-fluorescence; (4) albumin binding site characteristics. Screening of the individuals with a period of 25-26 years after the work in Chernobyl revealed two groups of patients differing in structural and functional albumin properties; first on conformations of plasma albumin, and second characteristics of tryptophanyl region of the molecule. The revealed structural modifications of albumin are dependent on radiation-induced factors. Concomitant diseases such as diabetes mellitus or cardio-vascular diseases reinforce radiation-induced effects. In conclusion, ABM is a sensitive probe for albumin alterations and can be used to elucidate the changes in protein systems. Significant differences in albumin dynamics exist between control (donors) and groups of Chernobyl clean-up workers.","PeriodicalId":16091,"journal":{"name":"Journal of Experimental and Integrative Medicine","volume":"35 1","pages":"165-170"},"PeriodicalIF":0.0,"publicationDate":"2014-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73151489","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2014-01-01DOI: 10.5455/JEIM.180913.OR.091
A. Kamal, D. Iskandriati, I. H. Dilogo, N. Siregar, E. Hutagalung, A. Yusuf, S. Mariya, Kurniadi Husodo
Objectives: Mesenchymal stem cells (MSC) are primarily isolated from bone marrow. Peripheral blood is also reported as an alternative source of MSC. This study compared MSC which were isolated and cultured from bone marrow and those from peripheral blood of rats. Methods: MSC from bone marrow and peripheral blood were harvested from 5 male Sprague Dawley rats. After isolation, the cells were grown on tissue culture plates with concentration of 107 cells per well. Observations were conducted to evaluate the attachment of nucleated cells with fibroblast-like morphology. Characterization of MSC was done using reverse transcriptase-polymerase chain reaction (RT-PCR) and immunocytochemistry assay. Results: The mean number of nucleated cells isolated from the bone marrow on day 0 was higher than those isolated from the peripheral blood. Bone marrow MSC with typical fibroblast-like morphology proliferated rapidly and reached 80% confluency on day 14. Subcultures were able to be conducted on day 15 (first passage) and day 28 (second passage). On days 15 and 28, no nucleated cells remained in peripheral blood cultures. Conclusions: Bone marrow derived MSC are more enriched and grow more efficiently than those derived from peripheral blood.
{"title":"Comparison of cultured mesenchymal stem cells derived from bone marrow or peripheral blood of rats","authors":"A. Kamal, D. Iskandriati, I. H. Dilogo, N. Siregar, E. Hutagalung, A. Yusuf, S. Mariya, Kurniadi Husodo","doi":"10.5455/JEIM.180913.OR.091","DOIUrl":"https://doi.org/10.5455/JEIM.180913.OR.091","url":null,"abstract":"Objectives: Mesenchymal stem cells (MSC) are primarily isolated from bone marrow. Peripheral blood is also reported as an alternative source of MSC. This study compared MSC which were isolated and cultured from bone marrow and those from peripheral blood of rats. Methods: MSC from bone marrow and peripheral blood were harvested from 5 male Sprague Dawley rats. After isolation, the cells were grown on tissue culture plates with concentration of 107 cells per well. Observations were conducted to evaluate the attachment of nucleated cells with fibroblast-like morphology. Characterization of MSC was done using reverse transcriptase-polymerase chain reaction (RT-PCR) and immunocytochemistry assay. Results: The mean number of nucleated cells isolated from the bone marrow on day 0 was higher than those isolated from the peripheral blood. Bone marrow MSC with typical fibroblast-like morphology proliferated rapidly and reached 80% confluency on day 14. Subcultures were able to be conducted on day 15 (first passage) and day 28 (second passage). On days 15 and 28, no nucleated cells remained in peripheral blood cultures. Conclusions: Bone marrow derived MSC are more enriched and grow more efficiently than those derived from peripheral blood.","PeriodicalId":16091,"journal":{"name":"Journal of Experimental and Integrative Medicine","volume":"110 3 1","pages":"17-22"},"PeriodicalIF":0.0,"publicationDate":"2014-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83071901","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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