This cross-sectional study aimed to investigate the association between skeletal bone mineral density (BMD) and periodontitis in Thai adults and elders.
Methods
This study comprised 3282 participants aged 30-82 years. BMD was assessed at 3 skeletal sites using dual-energy X-ray absorptiometry. Each participant's BMD status was classified as normal, osteopenia or osteoporosis. Periodontal assessments were the number of remaining teeth, plaque score, probing depth and clinical attachment level (CAL). The participants were classified into no/mild or moderate/severe periodontitis groups. The mean periodontal variables between BMD categories were compared. The association between the BMD status and moderate/severe periodontitis was analyzed using binary logistic regression.
Results
Among the BMD categories, the greatest mean CAL and the lowest mean number of remaining teeth were found in the osteoporosis group. The mean CAL difference between the osteoporosis and normal BMD groups was 0.3 mm. In 337 participants with a plaque score of less than 40%, there was a significant association between osteoporosis and moderate/severe periodontitis.
Conclusion
Skeletal BMD in the osteoporosis range was associated with moderate/severe periodontitis in individuals with fair oral hygiene, suggesting the benefit of special attention to the skeletal bone health of these individuals.
{"title":"Significant association between skeletal bone mineral density and moderate to severe periodontitis in fair oral hygiene individuals","authors":"Sanutm Mongkornkarn, Rapatchata Suthasinekul, Chanika Sritara, Attawood Lertpimonchai, Suphot Tamsailom, Artit Udomsak","doi":"10.1111/jicd.12441","DOIUrl":"10.1111/jicd.12441","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Aim</h3>\u0000 \u0000 <p>This cross-sectional study aimed to investigate the association between skeletal bone mineral density (BMD) and periodontitis in Thai adults and elders.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>This study comprised 3282 participants aged 30-82 years. BMD was assessed at 3 skeletal sites using dual-energy X-ray absorptiometry. Each participant's BMD status was classified as normal, osteopenia or osteoporosis. Periodontal assessments were the number of remaining teeth, plaque score, probing depth and clinical attachment level (CAL). The participants were classified into no/mild or moderate/severe periodontitis groups. The mean periodontal variables between BMD categories were compared. The association between the BMD status and moderate/severe periodontitis was analyzed using binary logistic regression.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>Among the BMD categories, the greatest mean CAL and the lowest mean number of remaining teeth were found in the osteoporosis group. The mean CAL difference between the osteoporosis and normal BMD groups was 0.3 mm. In 337 participants with a plaque score of less than 40%, there was a significant association between osteoporosis and moderate/severe periodontitis.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>Skeletal BMD in the osteoporosis range was associated with moderate/severe periodontitis in individuals with fair oral hygiene, suggesting the benefit of special attention to the skeletal bone health of these individuals.</p>\u0000 </section>\u0000 </div>","PeriodicalId":16204,"journal":{"name":"Journal of Investigative and Clinical Dentistry","volume":"10 4","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-07-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/jicd.12441","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49640639","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
To quantify the levels of miRNA-146a in subgingival plaque samples, and correlate with periodontal and cardiac parameters, in chronic periodontitis patients with and without coronary heart disease.
Methods
The study involved 90 subjects; 30 patients with chronic periodontitis and coronary heart disease (CP + CHD) as part of Group I; group II comprising 30 with chronic periodontitis alone (CP); and group III comprising 30 systemically healthy controls. Demographic variables, periodontal parameters such as plaque index, bleeding on probing, probing pocket depth and clinical attachment levels, cardiac parameters such as total cholesterol, high-density lipoprotein, low-density lipoprotein, triglyceride levels, and systolic and diastolic blood pressure were recorded from the patients. miRNA-146a level was analyzed in subgingival plaque samples by real-time polymerase chain reaction assay and correlated with periodontal and cardiac parameters.
Results
miRNA-146a showed the highest levels in the CP + CHD group and also showed a positive correlation with body mass index, and periodontal and cardiac parameters.
Conclusion
miRNA-146a is involved in the pathogenesis of both periodontitis and coronary heart disease.
{"title":"The Periodontal-Cardiovascular alliance: Evaluation of miRNA-146a in subgingival plaque samples of chronic periodontitis patients with and without coronary heart disease","authors":"Krutika Yagnik, Jaideep Mahendra, V. M. Kurian","doi":"10.1111/jicd.12442","DOIUrl":"10.1111/jicd.12442","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Aim</h3>\u0000 \u0000 <p>To quantify the levels of miRNA-146a in subgingival plaque samples, and correlate with periodontal and cardiac parameters, in chronic periodontitis patients with and without coronary heart disease.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>The study involved 90 subjects; 30 patients with chronic periodontitis and coronary heart disease (CP + CHD) as part of Group I; group II comprising 30 with chronic periodontitis alone (CP); and group III comprising 30 systemically healthy controls. Demographic variables, periodontal parameters such as plaque index, bleeding on probing, probing pocket depth and clinical attachment levels, cardiac parameters such as total cholesterol, high-density lipoprotein, low-density lipoprotein, triglyceride levels, and systolic and diastolic blood pressure were recorded from the patients. miRNA-146a level was analyzed in subgingival plaque samples by real-time polymerase chain reaction assay and correlated with periodontal and cardiac parameters.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>miRNA-146a showed the highest levels in the CP + CHD group and also showed a positive correlation with body mass index, and periodontal and cardiac parameters.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>miRNA-146a is involved in the pathogenesis of both periodontitis and coronary heart disease.</p>\u0000 </section>\u0000 </div>","PeriodicalId":16204,"journal":{"name":"Journal of Investigative and Clinical Dentistry","volume":"10 4","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-07-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/jicd.12442","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49109865","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Amol R. Gadbail, Minal S. Chaudhary, Sachin C. Sarode, Shailesh M. Gondivkar, Lalita Belekar, Mugdha P. Mankar-Gadbail, Ravi Dande, Satyajit A. Tekade, Monal B. Yuwanati, Shankargouda Patil
Aim
The aim of this study was to investigate the expression of Ki67, CD105 and α-smooth muscle actin (α-SMA) expression in oral submucous fibrosis (OSF) and oral squamous cell carcinoma in the background of OSF (OSCC-SMF).
Methods
The study was carried out on paraffin-embedded tissues of 30 normal oral mucosa (NOM), 50 OSF cases and 105 OSCC-SMF. The immunohistochemistry was carried out to evaluate the expression of Ki67, CD105 and α-SMA antigen.
Results
Ki67 labelling index (LI), CD105 and α-SMA expression showed increasing trend from NOM, low-risk epithelial dysplasia (LRED), high-risk epithelial dysplasia (HRED), well-differentiated squamous cell carcinoma (WDSCC), moderately differentiated squamous cell carcinoma to poorly differentiated squamous cell carcinoma. However, there was no significant difference of α-SMA expression between HRED and WDSCC. In OSCC-SMF, Ki67 LI, CD105 and α-SMA were significantly higher in advanced clinical TNM stage, metastasis and less than 3 years patient survival as compared with early clinical TNM stage, non-metastasis and 3 years or more patient survival.
Conclusion
Ki67 LI, α-SMA and CD105 expression alone or together correspond with the disease progression model of SMF. Hence, expression of these markers can be used as a predictive marker of clinical outcome of OSCC-SMF.
{"title":"Ki67, CD105 and α-smooth muscle actin expression in disease progression model of oral submucous fibrosis","authors":"Amol R. Gadbail, Minal S. Chaudhary, Sachin C. Sarode, Shailesh M. Gondivkar, Lalita Belekar, Mugdha P. Mankar-Gadbail, Ravi Dande, Satyajit A. Tekade, Monal B. Yuwanati, Shankargouda Patil","doi":"10.1111/jicd.12443","DOIUrl":"10.1111/jicd.12443","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Aim</h3>\u0000 \u0000 <p>The aim of this study was to investigate the expression of Ki67, CD105 and α-smooth muscle actin (α-SMA) expression in oral submucous fibrosis (OSF) and oral squamous cell carcinoma in the background of OSF (OSCC-SMF).</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>The study was carried out on paraffin-embedded tissues of 30 normal oral mucosa (NOM), 50 OSF cases and 105 OSCC-SMF. The immunohistochemistry was carried out to evaluate the expression of Ki67, CD105 and α-SMA antigen.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>Ki67 labelling index (LI), CD105 and α-SMA expression showed increasing trend from NOM, low-risk epithelial dysplasia (LRED), high-risk epithelial dysplasia (HRED), well-differentiated squamous cell carcinoma (WDSCC), moderately differentiated squamous cell carcinoma to poorly differentiated squamous cell carcinoma. However, there was no significant difference of α-SMA expression between HRED and WDSCC. In OSCC-SMF, Ki67 LI, CD105 and α-SMA were significantly higher in advanced clinical TNM stage, metastasis and less than 3 years patient survival as compared with early clinical TNM stage, non-metastasis and 3 years or more patient survival.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>Ki67 LI, α-SMA and CD105 expression alone or together correspond with the disease progression model of SMF. Hence, expression of these markers can be used as a predictive marker of clinical outcome of OSCC-SMF.</p>\u0000 </section>\u0000 </div>","PeriodicalId":16204,"journal":{"name":"Journal of Investigative and Clinical Dentistry","volume":"10 4","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-07-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/jicd.12443","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47224756","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The aim of the present study was to analyze the association between vitamin D receptor (VDR) (rs10735810) gene polymorphism and chronic periodontitis (CP).
Methods
A total of 100 subjects were recruited for this study, which included 50 CP and 50 healthy controls. Genomic DNA was extracted from the whole blood collected from the subjects. DNA was amplified using specific primers flanking the FokI region of the VDR gene (rs10735810). The amplicon was further subjected to genotyping using restriction fragment length polymorphism (RFLP) using the FokI enzyme. The genotype obtained based on RFLP pattern was recorded and used for statistical analysis. The distribution of genotypes and allele frequencies in the chronic periodontitis and control groups were compared using the χ2-test.
Results
The CP group displayed the highest frequency of CT (20%) and TT (6%) genotypes when compared with the control subjects. Allele frequency was found to be similar in both groups. The C allele was found to be predominant in the study population compared with the T allele.
Conclusion
The present study denotes that the VDR polymorphism (rs10735810) is not associated with CP in the study group analyzed.
{"title":"Association of vitamin D receptor gene polymorphism (rs10735810) and chronic periodontitis","authors":"Karthikeyan Murthykumar, Radhika Arjunkumar, Vijayashree Priyadharsini Jayaseelan","doi":"10.1111/jicd.12440","DOIUrl":"10.1111/jicd.12440","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Aim</h3>\u0000 \u0000 <p>The aim of the present study was to analyze the association between vitamin D receptor (<i>VDR</i>) (<i>rs10735810</i>) gene polymorphism and chronic periodontitis (CP).</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>A total of 100 subjects were recruited for this study, which included 50 CP and 50 healthy controls. Genomic DNA was extracted from the whole blood collected from the subjects. DNA was amplified using specific primers flanking the <i>FokI</i> region of the <i>VDR</i> gene (<i>rs10735810</i>). The amplicon was further subjected to genotyping using restriction fragment length polymorphism (RFLP) using the <i>FokI</i> enzyme. The genotype obtained based on RFLP pattern was recorded and used for statistical analysis. The distribution of genotypes and allele frequencies in the chronic periodontitis and control groups were compared using the χ<sup>2</sup>-test.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>The CP group displayed the highest frequency of CT (20%) and TT (6%) genotypes when compared with the control subjects. Allele frequency was found to be similar in both groups. The C allele was found to be predominant in the study population compared with the T allele.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>The present study denotes that the <i>VDR</i> polymorphism (<i>rs10735810</i>) is not associated with CP in the study group analyzed.</p>\u0000 </section>\u0000 </div>","PeriodicalId":16204,"journal":{"name":"Journal of Investigative and Clinical Dentistry","volume":"10 4","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-07-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/jicd.12440","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47437192","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Cícero R. Gadê-Neto, Ronaldo R. Rodrigues, Lidiane M. Louzada, Rodrigo Arruda-Vasconcelos, Fabrício B. Teixeira, Renato C. Viana Casarin, Brenda P. F. A. Gomes
Aim
To investigate the relationship between the microbiota of periodontal pockets (PP) and root canals (RC) in dogs submitted to experimental periodontal disease (ExPD).
Methods
ExPD was induced by combining cotton and wire ligatures. After 125 days, microbiological samples were collected from PP and RC. Strains isolated from 19 teeth were submitted to DNA extraction, 16S rRNA gene amplification and gene sequencing. Pearson's χ2- and Fisher's exact tests and McNemar's test were used when appropriate.
Results
The number of species in PP was greater than in RC, with prevalence of obligate anaerobes and Gram-negative bacteria. In the PP predominated Fusobacterium necrophorum, Porphyromonas gingivalis, Prevotella loescheii, Campylobacter gracilis and Veillonella parvula. In the RC samples, 9 had microbial growth, with predominance of the following genera: Staphylococcus, Streptococcus and Neisseria. Eight genera were common to both sites in the same tooth. PP presented a greater number of species than the RC. No significant difference was observed in the species found in PP and RC in the same tooth.
Conclusion
Microbial composition of the RC could be modulated by the presence of periodontal disease, especially in cases of severe periodontal destruction. RC microbiota was less complex and diverse than the PP.
{"title":"Microbiota of periodontal pockets and root canals in induced experimental periodontal disease in dogs","authors":"Cícero R. Gadê-Neto, Ronaldo R. Rodrigues, Lidiane M. Louzada, Rodrigo Arruda-Vasconcelos, Fabrício B. Teixeira, Renato C. Viana Casarin, Brenda P. F. A. Gomes","doi":"10.1111/jicd.12439","DOIUrl":"10.1111/jicd.12439","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Aim</h3>\u0000 \u0000 <p>To investigate the relationship between the microbiota of periodontal pockets (PP) and root canals (RC) in dogs submitted to experimental periodontal disease (ExPD).</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>ExPD was induced by combining cotton and wire ligatures. After 125 days, microbiological samples were collected from PP and RC. Strains isolated from 19 teeth were submitted to DNA extraction, 16S rRNA gene amplification and gene sequencing. Pearson's χ<sup>2</sup>- and Fisher's exact tests and McNemar's test were used when appropriate.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>The number of species in PP was greater than in RC, with prevalence of obligate anaerobes and Gram-negative bacteria. In the PP predominated <i>Fusobacterium necrophorum</i>, <i>Porphyromonas gingivalis</i>, <i>Prevotella loescheii</i>, <i>Campylobacter gracilis</i> and <i>Veillonella parvula</i>. In the RC samples, 9 had microbial growth, with predominance of the following genera: <i>Staphylococcus</i>, <i>Streptococcus</i> and <i>Neisseria</i>. Eight genera were common to both sites in the same tooth. PP presented a greater number of species than the RC. No significant difference was observed in the species found in PP and RC in the same tooth.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>Microbial composition of the RC could be modulated by the presence of periodontal disease, especially in cases of severe periodontal destruction. RC microbiota was less complex and diverse than the PP.</p>\u0000 </section>\u0000 </div>","PeriodicalId":16204,"journal":{"name":"Journal of Investigative and Clinical Dentistry","volume":"10 4","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-07-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/jicd.12439","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44644045","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The occurrence of oropharyngeal candidiasis (OPC) may be influenced by oral candidal carriage (OCC). Although OPC is strongly associated with low CD4+ cell count (400-700 cells/mm3) and a lack of highly active antiretroviral therapy (HAART), the effect of these two parameters on OCC is debatable. We investigated the oral candidal carriage, species diversity, antifungal susceptibility and the association of OCC with CD4+ cell count and HAART.
Methods
Oral candidal isolates from 120 HIV+ patients (60 receiving and 60 not receiving HAART) and 60 healthy controls were quantified, and their species determined using standard culture and biochemical methods, followed by antifungal susceptibility testing using the agar dilution method.
Results
The OCC was significantly higher in HIV+ patients; Candida albicans was the most frequently isolated species in both groups, followed by Candida tropicalis. Candidal density carriage correlated significantly with CD4+ cell count, but not with HIV and HAART status. Among the isolates from HIV+ patients, 35.4% showed reduced susceptibility to fluconazole.
Conclusion
HIV status results in significantly elevated rates of OCC C albicans remains the predominant pathogen, although other species are emerging rapidly. Resistance to fluconazole is on the rise, and more efficient treatment strategies need to be implemented.
{"title":"Oral candidal carriage correlates with CD4+ cell count but not with HIV and highly active antiretroviral therapy status","authors":"Parul Sah, Pratik Patel, Chetana Chandrashekar, Suganthi Martena, Mamatha Ballal, Manjayya Hegde, Vasudeva Guddattu, Craig Murdoch, Mohit Sharma, Raghu Radhakrishnan","doi":"10.1111/jicd.12438","DOIUrl":"10.1111/jicd.12438","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Aim</h3>\u0000 \u0000 <p>The occurrence of oropharyngeal candidiasis (OPC) may be influenced by oral candidal carriage (OCC). Although OPC is strongly associated with low CD4<sup>+</sup> cell count (400-700 cells/mm<sup>3</sup>) and a lack of highly active antiretroviral therapy (HAART), the effect of these two parameters on OCC is debatable. We investigated the oral candidal carriage, species diversity, antifungal susceptibility and the association of OCC with CD4<sup>+</sup> cell count and HAART.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>Oral candidal isolates from 120 HIV<sup>+</sup> patients (60 receiving and 60 not receiving HAART) and 60 healthy controls were quantified, and their species determined using standard culture and biochemical methods, followed by antifungal susceptibility testing using the agar dilution method.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>The OCC was significantly higher in HIV<sup>+</sup> patients; <i>Candida albicans</i> was the most frequently isolated species in both groups, followed by <i>Candida tropicalis</i>. Candidal density carriage correlated significantly with CD4<sup>+</sup> cell count, but not with HIV and HAART status. Among the isolates from HIV<sup>+</sup> patients, 35.4% showed reduced susceptibility to fluconazole.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>HIV status results in significantly elevated rates of OCC <i>C albicans</i> remains the predominant pathogen, although other species are emerging rapidly. Resistance to fluconazole is on the rise, and more efficient treatment strategies need to be implemented.</p>\u0000 </section>\u0000 </div>","PeriodicalId":16204,"journal":{"name":"Journal of Investigative and Clinical Dentistry","volume":"10 4","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-07-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/jicd.12438","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49071374","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Eugenio Pedullà, Carlo Genovese, Riccardo Messina, Giusy R. M. La Rosa, Giacomo Corsentino, Silvia Rapisarda, Maria T. Arias-Moliz, Gianna Tempera, Simone Grandini
Aim
The aim of the present study was to evaluate the immediate and residual antimicrobial activity of cordless sonic or ultrasonic devices on Enterococcus faecalis (E faecalis)-infected teeth.
Methods
A total of 140 single-rooted extracted teeth with E faecalis were grouped as follows (N = 15): conventional syringe irrigation with 3% sodium hypochlorite activated by ultrasonic device (group 1) or cordless ultrasonic device (group 2), EndoActivator (group 3) or without activation (group 4), conventional syringe irrigation with sterile bi-distilled water and ultrasonic device (group 5), EndoUltra (group 6), EndoActivator (group 7), or without activation (group 8). The remaining 20 teeth were used for positive and negative controls. Colony-forming units (CFU) and turbidity were recorded from bacteriological samples taken before and after irrigation and after 24 hours of re-incubation. Data were statistically analyzed using Student's t test, Mann-Whitney test, Kruskal-Wallis test, and Dunn's multiple comparison tests (P < .05).
Results
Groups 1 and 2 showed higher antimicrobial efficacy than groups 3 and 4 (P < .05). No bacteria reduction was found in groups 5-8 (P > .05). After 24 hours, regrowth of bacteria was not significantly different for all groups (P > .05).
Conclusions
Ultrasound produced lower CFU and turbidity after treatment and after re-incubation of 24 hours than sonic or no activation.
{"title":"Antimicrobial efficacy of cordless sonic or ultrasonic devices on Enterococcus faecalis-infected root canals","authors":"Eugenio Pedullà, Carlo Genovese, Riccardo Messina, Giusy R. M. La Rosa, Giacomo Corsentino, Silvia Rapisarda, Maria T. Arias-Moliz, Gianna Tempera, Simone Grandini","doi":"10.1111/jicd.12434","DOIUrl":"10.1111/jicd.12434","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Aim</h3>\u0000 \u0000 <p>The aim of the present study was to evaluate the immediate and residual antimicrobial activity of cordless sonic or ultrasonic devices on <i>Enterococcus faecalis</i> (<i>E faecalis</i>)-infected teeth.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>A total of 140 single-rooted extracted teeth with <i>E faecalis</i> were grouped as follows (N = 15): conventional syringe irrigation with 3% sodium hypochlorite activated by ultrasonic device (group 1) or cordless ultrasonic device (group 2), EndoActivator (group 3) or without activation (group 4), conventional syringe irrigation with sterile bi-distilled water and ultrasonic device (group 5), EndoUltra (group 6), EndoActivator (group 7), or without activation (group 8). The remaining 20 teeth were used for positive and negative controls. Colony-forming units (CFU) and turbidity were recorded from bacteriological samples taken before and after irrigation and after 24 hours of re-incubation. Data were statistically analyzed using Student's <i>t</i> test, Mann-Whitney test, Kruskal-Wallis test, and Dunn's multiple comparison tests (<i>P</i> < .05).</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>Groups 1 and 2 showed higher antimicrobial efficacy than groups 3 and 4 (<i>P</i> < .05). No bacteria reduction was found in groups 5-8 (<i>P</i> > .05). After 24 hours, regrowth of bacteria was not significantly different for all groups (<i>P</i> > .05).</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>Ultrasound produced lower CFU and turbidity after treatment and after re-incubation of 24 hours than sonic or no activation.</p>\u0000 </section>\u0000 </div>","PeriodicalId":16204,"journal":{"name":"Journal of Investigative and Clinical Dentistry","volume":"10 4","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-07-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/jicd.12434","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"37149861","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Amir Farmohammadi, Atefeh Tavangar, Mohammad Ehteram, Mohammad Karimian
Aim
The aim of the present study was to evaluate the association of interleukin-17 (IL-17) A-197G gene polymorphism with chronic periodontitis (CP) in a case-control study, a meta-analysis, and an in silico approach.
Methods
In the case-control study, 122 cases with CP and 126 healthy controls were recruited; IL-17 A-197G genotyping was performed by polymerase chain reaction-restriction fragment length polymorphism. In the meta-analysis, comprehensive literature retrieval was performed on valid databases to identify relevant studies. Bioinformatics tools were employed to investigate the effects of A-197G transition on the promoter region of IL-17.
Results
Our case-control study revealed a significant association between IL-17 A-197G transition and CP. The overall meta-analysis revealed significant associations between the IL-17 A-197G polymorphism and CP risk in homozygote co-dominant and recessive models. The stratified analysis also showed a statistically significant association between the mentioned transition and CP risk in the Caucasian population. The in silico analysis revealed that the A-197G polymorphism could make changes in protein-binding sites of the IL-17 promoter region.
Conclusions
Our study supports that IL-17 A-197G transition could be a genetic risk factor for CP. However, further studies with a larger sample size among different ethnicities are required to obtain a more accurate conclusion.
{"title":"Association of A-197G polymorphism in interleukin-17 gene with chronic periodontitis: Evidence from six case-control studies with a computational biology approach","authors":"Amir Farmohammadi, Atefeh Tavangar, Mohammad Ehteram, Mohammad Karimian","doi":"10.1111/jicd.12424","DOIUrl":"10.1111/jicd.12424","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Aim</h3>\u0000 \u0000 <p>The aim of the present study was to evaluate the association of interleukin-17 (<i>IL-17</i>) A-197G gene polymorphism with chronic periodontitis (CP) in a case-control study, a meta-analysis, and an in silico approach.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>In the case-control study, 122 cases with CP and 126 healthy controls were recruited; <i>IL-17</i> A-197G genotyping was performed by polymerase chain reaction-restriction fragment length polymorphism. In the meta-analysis, comprehensive literature retrieval was performed on valid databases to identify relevant studies. Bioinformatics tools were employed to investigate the effects of A-197G transition on the promoter region of <i>IL-17</i>.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>Our case-control study revealed a significant association between <i>IL-17</i> A-197G transition and CP. The overall meta-analysis revealed significant associations between the <i>IL-17</i> A-197G polymorphism and CP risk in homozygote co-dominant and recessive models. The stratified analysis also showed a statistically significant association between the mentioned transition and CP risk in the Caucasian population. The in silico analysis revealed that the A-197G polymorphism could make changes in protein-binding sites of the <i>IL-17</i> promoter region.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>Our study supports that <i>IL-17</i> A-197G transition could be a genetic risk factor for CP. However, further studies with a larger sample size among different ethnicities are required to obtain a more accurate conclusion.</p>\u0000 </section>\u0000 </div>","PeriodicalId":16204,"journal":{"name":"Journal of Investigative and Clinical Dentistry","volume":"10 4","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-06-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/jicd.12424","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"37096075","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
To investigate the correlation between the clinicopathological characteristics, serum antinuclear antibody (ANA) and direct immunofluorescence (DIF) findings in oral lichen planus (OLP) and oral lichenoid lesion (OLL).
Methods
Fifty three Thai patients with red and white lesions were divided into 3 groups: 17 cases of OLP, 19 cases of OLL and 17 cases of oral lichenoid drug reaction (OLDR), respectively. The medical records, photographs, histopathological evaluation and laboratory ANA and DIF results were analyzed.
Results
Atrophic pattern was the most commonly found pattern in the OLDR, OLP and OLL groups. In the OLP group, the DIF interpretation confirmed only 41.2% of cases as OLP, with 23.5% each as lichen planus (LP)/lupus erythematosus (LE) or negative findings. In the OLL group, the most common DIF interpretation (31.6% each) was LP/LE or non-specific finding. In the OLDR group, DIF interpretation was OLP or LP/LE (23.5% each), with 5.9% each of immune complex-mediated disease, compatible with OLP, and mixed connective tissue disease. Interestingly, 1 case in the OLDR group demonstrated mild to moderate dysplasia. There were no significant differences in ANA positivity or patterns between the 3 groups.
Conclusion
An OLP-like lesion could be diagnosed as OLP, OLP/LE, chronic ulcerative-like lesion, immune-mediated disease or dysplasia.
{"title":"Correlation of clinicopathological characteristics and direct immunofluorescence studies in oral lichenoid lesion in Thai patients","authors":"Kittiphoj Tikkhanarak, Daras Wangboo, Nichakorn Sookviboonpol, Kobkan Thongprasom","doi":"10.1111/jicd.12433","DOIUrl":"10.1111/jicd.12433","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Aim</h3>\u0000 \u0000 <p>To investigate the correlation between the clinicopathological characteristics, serum antinuclear antibody (ANA) and direct immunofluorescence (DIF) findings in oral lichen planus (OLP) and oral lichenoid lesion (OLL).</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>Fifty three Thai patients with red and white lesions were divided into 3 groups: 17 cases of OLP, 19 cases of OLL and 17 cases of oral lichenoid drug reaction (OLDR), respectively. The medical records, photographs, histopathological evaluation and laboratory ANA and DIF results were analyzed.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>Atrophic pattern was the most commonly found pattern in the OLDR, OLP and OLL groups. In the OLP group, the DIF interpretation confirmed only 41.2% of cases as OLP, with 23.5% each as lichen planus (LP)/lupus erythematosus (LE) or negative findings. In the OLL group, the most common DIF interpretation (31.6% each) was LP/LE or non-specific finding. In the OLDR group, DIF interpretation was OLP or LP/LE (23.5% each), with 5.9% each of immune complex-mediated disease, compatible with OLP, and mixed connective tissue disease. Interestingly, 1 case in the OLDR group demonstrated mild to moderate dysplasia. There were no significant differences in ANA positivity or patterns between the 3 groups.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>An OLP-like lesion could be diagnosed as OLP, OLP/LE, chronic ulcerative-like lesion, immune-mediated disease or dysplasia.</p>\u0000 </section>\u0000 </div>","PeriodicalId":16204,"journal":{"name":"Journal of Investigative and Clinical Dentistry","volume":"10 4","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-06-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/jicd.12433","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"37072596","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Nathamon Thongbai-on, Kanet Chotvorrarak, Danuchit Banomyong, Michael F. Burrow, Sittichoke Osiri, Nattha Pattaravisitsate
Aim
To evaluate fracture resistance and gap/void presence of root-filled mandibular molars restored with 2 bulk-fill and 1 conventional resin composites, with or without a glass-ionomer cement (GIC) base.
Methods
Coronal access and mesio-occlusal (MO) cavities were prepared, then root canal treatment was performed on 30 mol/L. The teeth were randomly divided, according to the cavity volume, into 6 experimental groups (N = 5) and restored with conventional/light-cured (Ceram-X), bulk-fill/light-cured (SureFil SDR) or bulk-fill/dual-cured (Core-X Flow) with/without a 2-mm thick GIC base. Gaps and voids (%) were determined using microcomputed tomography. Intact teeth and unrestored teeth were used as negative and positive controls. Fracture load (N) was determined using a universal testing machine.
Results
No significant difference in fracture resistance or gap/void formation was found among the 3 resin composites. GIC-base groups revealed significantly lower fracture strength than intact teeth, while fracture strengths of no GIC-base groups were not significantly different from intact teeth. GIC-base groups revealed significantly more gaps and voids in the area of the GIC than the resin composite.
Conclusion
Conventional and bulk-fill resin composites provided similar fracture resistance and gaps/voids in root-filled molars with MO cavities. Placing a GIC base decreased fracture resistance and increased gap/void formation.
{"title":"Fracture resistance, gap and void formation in root-filled mandibular molars restored with bulk-fill resin composites and glass-ionomer cement base","authors":"Nathamon Thongbai-on, Kanet Chotvorrarak, Danuchit Banomyong, Michael F. Burrow, Sittichoke Osiri, Nattha Pattaravisitsate","doi":"10.1111/jicd.12435","DOIUrl":"10.1111/jicd.12435","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Aim</h3>\u0000 \u0000 <p>To evaluate fracture resistance and gap/void presence of root-filled mandibular molars restored with 2 bulk-fill and 1 conventional resin composites, with or without a glass-ionomer cement (GIC) base.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>Coronal access and mesio-occlusal (MO) cavities were prepared, then root canal treatment was performed on 30 mol/L. The teeth were randomly divided, according to the cavity volume, into 6 experimental groups (N = 5) and restored with conventional/light-cured (Ceram-X), bulk-fill/light-cured (SureFil SDR) or bulk-fill/dual-cured (Core-X Flow) with/without a 2-mm thick GIC base. Gaps and voids (%) were determined using microcomputed tomography. Intact teeth and unrestored teeth were used as negative and positive controls. Fracture load (N) was determined using a universal testing machine.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>No significant difference in fracture resistance or gap/void formation was found among the 3 resin composites. GIC-base groups revealed significantly lower fracture strength than intact teeth, while fracture strengths of no GIC-base groups were not significantly different from intact teeth. GIC-base groups revealed significantly more gaps and voids in the area of the GIC than the resin composite.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>Conventional and bulk-fill resin composites provided similar fracture resistance and gaps/voids in root-filled molars with MO cavities. Placing a GIC base decreased fracture resistance and increased gap/void formation.</p>\u0000 </section>\u0000 </div>","PeriodicalId":16204,"journal":{"name":"Journal of Investigative and Clinical Dentistry","volume":"10 4","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/jicd.12435","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"37343149","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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