Journal of Investigative and Clinical Dentistry最新文献_第4页

Oral mucositis and microbial colonization in oral cancer patients undergoing radiotherapy and chemotherapy: A prospective analysis in a tertiary care dental hospital 接受放疗和化疗的口腔癌症患者的口腔粘膜炎和微生物定植：三级牙科医院的前瞻性分析。

Q1 Medicine

Journal of Investigative and Clinical Dentistry

Pub Date : 2019-08-27 DOI: 10.1111/jicd.12454

Nandhini Subramaniam, Arvind Muthukrishnan

Aim

The ulcerative phase of oral mucositis following radiotherapy/chemotherapy for oral cancer colonizes bacteria, fungi and viruses. The role of a microbiota, specifically bacterial colonization in oral mucositis, is still unclear, and there is no existing data that correlates the shift in the bacterial colonization with mucositis severity. The aim of this study was to assess the bacterial colonization and study the MCR-1 (mobilized colistin resistance), VIM2 (β-lactam resistance), TET(K) (tetracycline resistance) and bla_KPC (carbapenem resistance) genes’ expression in isolated facultative anaerobes at 3 time points in oral mucositis patients undergoing radiotherapy and concomitant radiochemotherapy.

Methods

A total of 24 oral cancer patients were divided into 2 groups: A (N = 12) undergoing radiotherapy; and B (N = 12) undergoing radiochemotherapy. Saliva was collected from all patients at 3 time intervals during the treatment. The isolated bacterial colonies were subjected to gene expression and analysis.

Results

Staphylococcus aureus (22%), Staphylococcus epidermidis (29%), Pseudomonas aeruginosa (28%), Escherichia coli (25%) and Klebsiella pneumoniae (26%) are the facultative anaerobes isolated from saliva. The bacterial isolates obtained during and at the end of therapy appeared to express a higher level of antibiotic-resistance genes (VIM2, MCR-1, TET[K], bla_KPC) than those isolated at the onset of therapy.

Conclusion

Bacterial colonization and gene expression varied during different stages of mucositis.

目的口腔癌症放疗/化疗后口腔粘膜炎溃疡期细菌、真菌和病毒定植。微生物群，特别是细菌定殖在口腔粘膜炎中的作用尚不清楚，也没有现有数据将细菌定殖的变化与粘膜炎的严重程度联系起来。本研究的目的是评估细菌定植，并研究MCR-1（动员粘菌素耐药性）、VIM2（β-内酰胺耐药性）、TET（K）（四环素耐药性）和blaKPC（碳青霉烯耐药性）基因在接受放疗和联合放化疗的口腔粘膜炎患者的分离兼性厌氧菌中的3个时间点的表达。方法将24例口腔癌症患者分为2组：A组（N=12）接受放疗；B（N=12）正在接受放化疗。在治疗过程中，每隔3个时间间隔采集所有患者的唾液。对分离的菌落进行基因表达和分析。结果金黄色葡萄球菌（22%）、表皮葡萄球菌（29%）、铜绿假单胞菌（28%）、大肠杆菌（25%）和肺炎克雷伯菌（26%）是从唾液中分离的兼性厌氧菌。在治疗期间和治疗结束时获得的细菌分离株似乎比治疗开始时分离的菌株表达更高水平的抗生素耐药性基因（VIM2、MCR-1、TET[K]、blaKPC）。结论粘膜炎不同阶段细菌定殖及基因表达不同。

{"title":"Oral mucositis and microbial colonization in oral cancer patients undergoing radiotherapy and chemotherapy: A prospective analysis in a tertiary care dental hospital","authors":"Nandhini Subramaniam, Arvind Muthukrishnan","doi":"10.1111/jicd.12454","DOIUrl":"10.1111/jicd.12454","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Aim</h3>\u0000 \u0000 <p>The ulcerative phase of oral mucositis following radiotherapy/chemotherapy for oral cancer colonizes bacteria, fungi and viruses. The role of a microbiota, specifically bacterial colonization in oral mucositis, is still unclear, and there is no existing data that correlates the shift in the bacterial colonization with mucositis severity. The aim of this study was to assess the bacterial colonization and study the <i>MCR-1</i> (mobilized colistin resistance), <i>VIM2</i> (β-lactam resistance), <i>TET(K) </i>(tetracycline resistance) and <i>bla</i><sub><i>KPC</i></sub> (carbapenem resistance) genes’ expression in isolated facultative anaerobes at 3 time points in oral mucositis patients undergoing radiotherapy and concomitant radiochemotherapy.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>A total of 24 oral cancer patients were divided into 2 groups: A (N = 12) undergoing radiotherapy; and B (N = 12) undergoing radiochemotherapy. Saliva was collected from all patients at 3 time intervals during the treatment. The isolated bacterial colonies were subjected to gene expression and analysis.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p><i>Staphylococcus aureus</i> (22%), <i>Staphylococcus epidermidis</i> (29%), <i>Pseudomonas aeruginosa</i> (28%), <i>Escherichia coli</i> (25%) and <i>Klebsiella pneumoniae</i> (26%) are the facultative anaerobes isolated from saliva. The bacterial isolates obtained during and at the end of therapy appeared to express a higher level of antibiotic-resistance genes (<i>VIM2</i>, <i>MCR-1</i>, <i>TET[K]</i>, <i>bla</i><sub><i>KPC</i></sub>) than those isolated at the onset of therapy.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>Bacterial colonization and gene expression varied during different stages of mucositis.</p>\u0000 </section>\u0000 </div>","PeriodicalId":16204,"journal":{"name":"Journal of Investigative and Clinical Dentistry","volume":"10 4","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-08-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/jicd.12454","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46795497","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 76

Explanation for different behavior of peripheral and central giant cell granuloma by CD44 and CD34 immunostaining CD44和CD34免疫染色解释外周和中心巨细胞肉芽肿的不同行为。

Q1 Medicine

Journal of Investigative and Clinical Dentistry

Pub Date : 2019-08-21 DOI: 10.1111/jicd.12451

Massoumeh Zargaran, Fahimeh Baghaei, Abbas Moghimbeigi, Fereshteh Baghai

Aim

Despite their histopathological similarity, unlike peripheral giant cell granuloma (PGCG), central giant cell granuloma (CGCG) is an osteolytic lesion. Low motility of osteoclasts as bone-resorbing agents decreases osteolysis where CD44 plays a role. The lesion requires angiogenesis to grow which in turn may cause bone resorption. This study aims to compare CD44 and CD34 expression in PGCG and CGCG.

Methods

30 PGCG (group A) and 30 CGCG (group B) including non-aggressive (B1, N = 14) and aggressive (B2, N = 16) subgroups were evaluated for CD44 and CD34 expression through immunohistochemistry.

Results

CD44 staining intensity distribution (SID) score and CD44 labelling index (LI) in PGCG were significantly higher than those in CGCG, while microvessel density assessed by CD34 (MVD-CD34) was significantly higher in CGCG compared with PGCG. CD44-SID score and CD44-LI were higher for B1 whereas MVD-CD34 was higher for B2 (P < .05).

Conclusion

Different CD44 expression among the studied groups may be indicative of the different motility of osteoclastic giant cells which may influence bone resorption. Lower CD44 expression probably indicates higher osteoclastic giant cell motility in CGCG, which with its higher angiogenesis may explain the different clinical behavior of CGCG compared with that of PGCG.

目的尽管其组织病理学相似，但与外周巨细胞肉芽肿（PGCG）不同，中央巨细胞肉芽肿（CGCG）是一种溶骨性病变。破骨细胞作为骨吸收剂的低运动性降低了CD44发挥作用的骨溶解。病变需要血管生成才能生长，这反过来可能导致骨吸收。本研究旨在比较CD44和CD34在PGCG和CGCG中的表达。通过免疫组织化学方法评估30个PGCG（A组）和30个CGCG（B组）（包括非侵袭性（B1，N=14）和侵袭性（B2，N=16）亚组的CD44和CD34表达。结果PGCG的CD44染色强度分布（SID）评分和CD44标记指数（LI）显著高于CGCG，而CD34评估的微血管密度（MVD-CD34）显著高于PGCG。CD44-SID评分和CD44-LI在B1组中较高，而MVD-CD34在B2组中较高（P＜0.05）。结论研究组中CD44的不同表达可能表明破骨细胞巨细胞的不同运动性可能影响骨吸收。较低的CD44表达可能表明CGCG中破骨细胞巨细胞的运动性较高，其较高的血管生成可能解释了CGCG与PGCG不同的临床行为。

{"title":"Explanation for different behavior of peripheral and central giant cell granuloma by CD44 and CD34 immunostaining","authors":"Massoumeh Zargaran, Fahimeh Baghaei, Abbas Moghimbeigi, Fereshteh Baghai","doi":"10.1111/jicd.12451","DOIUrl":"10.1111/jicd.12451","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Aim</h3>\u0000 \u0000 <p>Despite their histopathological similarity, unlike peripheral giant cell granuloma (PGCG), central giant cell granuloma (CGCG) is an osteolytic lesion. Low motility of osteoclasts as bone-resorbing agents decreases osteolysis where CD44 plays a role. The lesion requires angiogenesis to grow which in turn may cause bone resorption. This study aims to compare CD44 and CD34 expression in PGCG and CGCG.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>30 PGCG (group A) and 30 CGCG (group B) including non-aggressive (B1, N = 14) and aggressive (B2, N = 16) subgroups were evaluated for CD44 and CD34 expression through immunohistochemistry.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>CD44 staining intensity distribution (SID) score and CD44 labelling index (LI) in PGCG were significantly higher than those in CGCG, while microvessel density assessed by CD34 (MVD-CD34) was significantly higher in CGCG compared with PGCG. CD44-SID score and CD44-LI were higher for B1 whereas MVD-CD34 was higher for B2 (<i>P </i>< .05).</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>Different CD44 expression among the studied groups may be indicative of the different motility of osteoclastic giant cells which may influence bone resorption. Lower CD44 expression probably indicates higher osteoclastic giant cell motility in CGCG, which with its higher angiogenesis may explain the different clinical behavior of CGCG compared with that of PGCG.</p>\u0000 </section>\u0000 </div>","PeriodicalId":16204,"journal":{"name":"Journal of Investigative and Clinical Dentistry","volume":"10 4","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-08-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/jicd.12451","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48089305","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Socket-shield technique for implant placement to stabilize the facial gingival and osseous architecture: A systematic review 牙槽-屏蔽技术用于种植体植入以稳定面部牙龈和骨骼结构:系统综述。

Q1 Medicine

Journal of Investigative and Clinical Dentistry

Pub Date : 2019-08-21 DOI: 10.1111/jicd.12449

Akanksha Mourya, Sunil Kumar Mishra, Reetika Gaddale, Ramesh Chowdhary

The aim of the present study was to establish the efficacy of the socket-shield technique (SST) for the stabilization of the facial gingival and osseous architecture. An electronic search including the Cochrane databases, EBSCOhost, Medline/PubMed and Web of Science was performed. Articles related to SST for placing dental implants were included. Articles and abstracts not written in English were excluded. The initial literature search resulted in 113 articles related to questions raised. Hand searching of the journals related to implants and cross-referencing related to SST within the selected articles resulted in 1 more paper. Finally, 20 full texts and abstract of 1 article were included in the present systematic review: 11 case reports, 6 case series, 1 human randomized control trial (RCT), 1 technical report and 2 animal RCT. Recent modifications in SST, along with long follow-up studies with increased sample size, provided promising results. This systematic review still recommends that SST should not be used in routine clinical practise until a higher level of evidence established. Further RCT on SST are required to establish the clinical efficacy of this technique.

本研究的目的是建立牙槽-屏蔽技术(SST)对面部牙龈和骨结构稳定的效果。电子检索包括Cochrane数据库、EBSCOhost、Medline/PubMed和Web of Science。包括与放置种植体的SST相关的文章。非英文的文章和摘要被排除在外。最初的文献检索结果是113篇与提出的问题相关的文章。手工检索与植入物相关的期刊，并在选定的文章中交叉引用与海温相关的文献，结果多出了1篇论文。最后，本系统综述纳入了20篇全文和1篇文章摘要:11篇病例报告、6篇病例系列、1篇人类随机对照试验(RCT)、1篇技术报告和2篇动物随机对照试验。最近对SST的修改，以及增加样本量的长期随访研究，提供了有希望的结果。本系统综述仍然建议，在获得更高水平的证据之前，SST不应用于常规临床实践。需要对SST进行进一步的随机对照试验以确定该技术的临床疗效。

{"title":"Socket-shield technique for implant placement to stabilize the facial gingival and osseous architecture: A systematic review","authors":"Akanksha Mourya, Sunil Kumar Mishra, Reetika Gaddale, Ramesh Chowdhary","doi":"10.1111/jicd.12449","DOIUrl":"10.1111/jicd.12449","url":null,"abstract":"<p>The aim of the present study was to establish the efficacy of the socket-shield technique (SST) for the stabilization of the facial gingival and osseous architecture. An electronic search including the Cochrane databases, EBSCOhost, Medline/PubMed and Web of Science was performed. Articles related to SST for placing dental implants were included. Articles and abstracts not written in English were excluded. The initial literature search resulted in 113 articles related to questions raised. Hand searching of the journals related to implants and cross-referencing related to SST within the selected articles resulted in 1 more paper. Finally, 20 full texts and abstract of 1 article were included in the present systematic review: 11 case reports, 6 case series, 1 human randomized control trial (RCT), 1 technical report and 2 animal RCT. Recent modifications in SST, along with long follow-up studies with increased sample size, provided promising results. This systematic review still recommends that SST should not be used in routine clinical practise until a higher level of evidence established. Further RCT on SST are required to establish the clinical efficacy of this technique.</p>","PeriodicalId":16204,"journal":{"name":"Journal of Investigative and Clinical Dentistry","volume":"10 4","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-08-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/jicd.12449","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47810998","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 31

Oral lesions and associated factors in breast cancer survivors 乳腺癌幸存者的口腔病变及相关因素

Q1 Medicine

Journal of Investigative and Clinical Dentistry

Pub Date : 2019-08-12 DOI: 10.1111/jicd.12447

Luísa Comerlato Jardim, Priscila Trindade Flores, Felipe de Araújo Sensever, Maria do Carmo dos Santos Araújo, Cristina Machado Bragança de Moraes, Juares Chiesa, Raquel Pippi Antoniazzi

Aim

To evaluate the prevalence of oral lesions (OL) and associated factors among survivors of breast cancer (BC).

Methods

A cross-sectional study involving 150 BC survivors was conducted at a public hospital in southern Brazil. Data were collected on socioeconomic aspects, treatment characteristics and oral problems. The decayed, missing and filled teeth index and the occurrence of OL were evaluated. Logistic regression was performed to determine independent variables associated with the outcome.

Results

24% of the women had at least 1 OL and 33.3% of these had more than one lesion. Melanotic macule was the most prevalent lesion. Duration of tamoxifen use, radiotherapy, missing teeth and xerostomia were associated with the occurrence of OL (P < .05). In the adjusted analysis, women with more than 13 missing teeth and xerostomia had 2.39-fold (95% confidence interval [CI], 1.06-5.40) and 2.71-fold (95% CI, 1.14-6.42), respectively, greater odds of exhibiting OL.

Conclusion

Approximately 1/4 of the BC survivors exhibited OL, which were associated with tooth loss and xerostomia. These findings could assist in the establishment of oral health strategies for women with BC.

目的评估癌症幸存者口腔病变（OL）的患病率及其相关因素。方法在巴西南部一家公立医院对150名乳腺癌幸存者进行横断面研究。收集了有关社会经济方面、治疗特点和口腔问题的数据。评估蛀牙、缺牙和补牙指数以及OL的发生情况。进行Logistic回归以确定与结果相关的自变量。结果24%的女性至少有1个OL，33.3%的女性有一个以上的病变。黑色素性黄斑是最常见的病变。他莫昔芬的使用时间、放疗、缺牙和口干症与OL的发生有关（P＜0.05）。在调整后的分析中，缺牙和口干症超过13颗的女性表现出OL的几率分别为2.39倍（95%置信区间[CI]，1.06-5.40）和2.71倍（95%CI，1.14-6.42）。结论约1/4的BC幸存者表现出OL，其与牙齿缺失和口干症有关。这些发现可能有助于为患有BC的妇女制定口腔健康策略。

{"title":"Oral lesions and associated factors in breast cancer survivors","authors":"Luísa Comerlato Jardim, Priscila Trindade Flores, Felipe de Araújo Sensever, Maria do Carmo dos Santos Araújo, Cristina Machado Bragança de Moraes, Juares Chiesa, Raquel Pippi Antoniazzi","doi":"10.1111/jicd.12447","DOIUrl":"10.1111/jicd.12447","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Aim</h3>\u0000 \u0000 <p>To evaluate the prevalence of oral lesions (OL) and associated factors among survivors of breast cancer (BC).</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>A cross-sectional study involving 150 BC survivors was conducted at a public hospital in southern Brazil. Data were collected on socioeconomic aspects, treatment characteristics and oral problems. The decayed, missing and filled teeth index and the occurrence of OL were evaluated. Logistic regression was performed to determine independent variables associated with the outcome.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>24% of the women had at least 1 OL and 33.3% of these had more than one lesion. Melanotic macule was the most prevalent lesion. Duration of tamoxifen use, radiotherapy, missing teeth and xerostomia were associated with the occurrence of OL (<i>P</i> < .05). In the adjusted analysis, women with more than 13 missing teeth and xerostomia had 2.39-fold (95% confidence interval [CI], 1.06-5.40) and 2.71-fold (95% CI, 1.14-6.42), respectively, greater odds of exhibiting OL.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>Approximately 1/4 of the BC survivors exhibited OL, which were associated with tooth loss and xerostomia. These findings could assist in the establishment of oral health strategies for women with BC.</p>\u0000 </section>\u0000 </div>","PeriodicalId":16204,"journal":{"name":"Journal of Investigative and Clinical Dentistry","volume":"10 4","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-08-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/jicd.12447","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42011688","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Efficacy of gabapentin in the treatment of trigeminal neuralgia: A systematic review of randomized controlled trials 加巴喷丁治疗三叉神经痛的疗效：随机对照试验的系统评价。

Q1 Medicine

Journal of Investigative and Clinical Dentistry

Pub Date : 2019-08-11 DOI: 10.1111/jicd.12448

Patrick Chieu Poa Ta, Hue Quyen Dinh, Kim Nguyen, Samuel Lin, Yong Li Ong, Anura Ariyawardana

The aim of this systematic review was to determine the efficacy of gabapentin (GBP) in the treatment of pain of idiopathic trigeminal neuralgia (TN). A comprehensive literature search was conducted using the Cumulative Index of Nursing and Allied Health Literature (EBSCO Industries), Emcare (Ovid), Medline (Ovid), Medline (PubMed), Scopus (Elsevier) and Web of Science (Clarivate Analytics). The inclusion criteria comprised randomized controlled trials of GBP as a monotherapy in the treatment of idiopathic TN in adult participants and publications in English. All other study methodologies were excluded. The search yielded 1472 articles, and after exclusion, 11 full-text articles were eligible for full-text analysis. Only two studies met the inclusion criteria. There is insufficient evidence either to support or refute the efficacy of GBP in the management of idiopathic TN. Therefore, further well-designed placebo-controlled trials are required to confirm the efficacy of GBP in managing TN pain as a single therapy.

本系统综述的目的是确定加巴喷丁(GBP)治疗特发性三叉神经痛(TN)疼痛的疗效。使用护理和联合健康文献累积索引(EBSCO Industries)、Emcare (Ovid)、Medline (Ovid)、Medline (PubMed)、Scopus(爱思唯尔)和Web of Science (Clarivate Analytics)进行了全面的文献检索。纳入标准包括GBP作为成人特发性TN单药治疗的随机对照试验和英文出版物。所有其他研究方法均被排除在外。检索得到1472篇文章，排除后，有11篇全文文章符合全文分析的条件。只有两项研究符合纳入标准。没有足够的证据支持或反驳GBP治疗特发性TN的有效性。因此，需要进一步设计良好的安慰剂对照试验来证实GBP作为单一疗法治疗TN疼痛的有效性。

{"title":"Efficacy of gabapentin in the treatment of trigeminal neuralgia: A systematic review of randomized controlled trials","authors":"Patrick Chieu Poa Ta, Hue Quyen Dinh, Kim Nguyen, Samuel Lin, Yong Li Ong, Anura Ariyawardana","doi":"10.1111/jicd.12448","DOIUrl":"10.1111/jicd.12448","url":null,"abstract":"<p>The aim of this systematic review was to determine the efficacy of gabapentin (GBP) in the treatment of pain of idiopathic trigeminal neuralgia (TN). A comprehensive literature search was conducted using the Cumulative Index of Nursing and Allied Health Literature (EBSCO Industries), Emcare (Ovid), Medline (Ovid), Medline (PubMed), Scopus (Elsevier) and Web of Science (Clarivate Analytics). The inclusion criteria comprised randomized controlled trials of GBP as a monotherapy in the treatment of idiopathic TN in adult participants and publications in English. All other study methodologies were excluded. The search yielded 1472 articles, and after exclusion, 11 full-text articles were eligible for full-text analysis. Only two studies met the inclusion criteria. There is insufficient evidence either to support or refute the efficacy of GBP in the management of idiopathic TN. Therefore, further well-designed placebo-controlled trials are required to confirm the efficacy of GBP in managing TN pain as a single therapy.</p>","PeriodicalId":16204,"journal":{"name":"Journal of Investigative and Clinical Dentistry","volume":"10 4","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-08-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/jicd.12448","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44366853","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 6

Clinical and patient-centered outcomes post non-surgical periodontal therapy with the use of a non-injectable anesthetic product: A randomized clinical study 临床和以患者为中心的非手术牙周治疗后使用非注射麻醉剂的结果:一项随机临床研究

Q1 Medicine

Journal of Investigative and Clinical Dentistry

Pub Date : 2019-07-28 DOI: 10.1111/jicd.12446

Simone Marconcini, Marilyn Goulding, Giacomo Oldoini, Chiara Attanasio, Enrica Giammarinaro, Annamaria Genovesi

Aim

The aim of this study was to determine the impact of different full-mouth decontamination (FMD) protocols on the effectiveness of an intrapocket anesthetic gel in periodontal maintenance patients.

Methods

Patients undergoing the periodontal maintenance program and with the need for FMD participated in this study. Patients were randomly allocated to non-surgical periodontal therapy (NSPT) with either a preparatory 15-day decontamination phase, including chlorhexidine mouth rinse and domiciliary hygiene instructions (modified FMD: test group), or without it (FMD: control group). In both groups, NSPT was performed with the aid of a non-injectable anesthetic gel. Clinical and patient-related outcomes were recorded during a 6-month follow-up period.

Results

Sixty patients completed the 6-month study. Both groups experienced relevant clinical improvements after NSPT, but the test group showed a significant change in periodontal parameters already after the initial 15-day preparatory period, and overall significantly better results in periodontal outcomes when compared with the control group at the last 6-month follow up: the gingival index was 2.07 ± 1.25 in the control group and 1.13 ± 0.51 in the test group. Less pain and dental-related anxiety were perceived by patients in the test group showing a 6-month mean visual analog scale of 2.13 ± 1.25 in the control group and 1.13 ± 0.83 in the test group.

Conclusion

The present study suggested that the modification of the standard FMD could improve the clinical efficacy of non-injectable anesthetic, along with patients’ short- and mid-term appreciation and compliance.

摘要目的本研究的目的是确定不同的全口去污(FMD)方案对牙周维持患者包内麻醉凝胶有效性的影响。方法对正在进行牙周维护计划并需要FMD的患者进行研究。患者被随机分配到非手术牙周治疗组(NSPT)，其中包括15天的预备去污阶段，包括洗必泰漱口水和住所卫生指导(改良FMD:试验组)，或不进行(FMD:对照组)。在两组中，NSPT都是在非注射麻醉凝胶的帮助下进行的。在6个月的随访期间记录临床和患者相关的结果。60例患者完成了为期6个月的研究。两组在NSPT后均有相应的临床改善，但试验组在最初的15天准备期后牙周参数已经发生了显著变化，并且在最后6个月的随访中，牙周结果总体上明显优于对照组:对照组的牙龈指数为2.07±1.25，试验组为1.13±0.51。试验组患者的疼痛感和牙齿相关焦虑减轻，对照组6个月平均视觉模拟量表为2.13±1.25，试验组为1.13±0.83。结论标准FMD的修改可提高非注射麻醉剂的临床疗效，提高患者对非注射麻醉剂的短期和中期评价和依从性。

{"title":"Clinical and patient-centered outcomes post non-surgical periodontal therapy with the use of a non-injectable anesthetic product: A randomized clinical study","authors":"Simone Marconcini, Marilyn Goulding, Giacomo Oldoini, Chiara Attanasio, Enrica Giammarinaro, Annamaria Genovesi","doi":"10.1111/jicd.12446","DOIUrl":"10.1111/jicd.12446","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Aim</h3>\u0000 \u0000 <p>The aim of this study was to determine the impact of different full-mouth decontamination (FMD) protocols on the effectiveness of an intrapocket anesthetic gel in periodontal maintenance patients.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>Patients undergoing the periodontal maintenance program and with the need for FMD participated in this study. Patients were randomly allocated to non-surgical periodontal therapy (NSPT) with either a preparatory 15-day decontamination phase, including chlorhexidine mouth rinse and domiciliary hygiene instructions (modified FMD: test group), or without it (FMD: control group). In both groups, NSPT was performed with the aid of a non-injectable anesthetic gel. Clinical and patient-related outcomes were recorded during a 6-month follow-up period.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>Sixty patients completed the 6-month study. Both groups experienced relevant clinical improvements after NSPT, but the test group showed a significant change in periodontal parameters already after the initial 15-day preparatory period, and overall significantly better results in periodontal outcomes when compared with the control group at the last 6-month follow up: the gingival index was 2.07 ± 1.25 in the control group and 1.13 ± 0.51 in the test group. Less pain and dental-related anxiety were perceived by patients in the test group showing a 6-month mean visual analog scale of 2.13 ± 1.25 in the control group and 1.13 ± 0.83 in the test group.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>The present study suggested that the modification of the standard FMD could improve the clinical efficacy of non-injectable anesthetic, along with patients’ short- and mid-term appreciation and compliance.</p>\u0000 </section>\u0000 </div>","PeriodicalId":16204,"journal":{"name":"Journal of Investigative and Clinical Dentistry","volume":"10 4","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-07-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/jicd.12446","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46720478","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

Topical insulin-liposomal formulation in management of recurrent aphthous ulcers: A randomized placebo-controlled trial 局部胰岛素脂质体制剂治疗复发性阿弗他溃疡：一项随机安慰剂对照试验。

Q1 Medicine

Journal of Investigative and Clinical Dentistry

Pub Date : 2019-07-27 DOI: 10.1111/jicd.12437

Naglaa M. El-Wakeel, Nadia M. Morsi, Dalia M. Ghorab, Ghada E. Yassin, Marwa H. S. Dawoud

Aim

To evaluate the effectiveness of topical insulin-liposomal gel in aphthous ulcer treatment.

Methods

80 participants with minor aphthous ulcers were randomly divided to receive either topical insulin-liposomal gel or placebo gel (once daily) for 6 days. Assessment of outcomes included visual analog scale (VAS) for pain (primary outcome), and secondary outcomes included ulcer duration and impact of treatment on quality of life using the Oral Health Impact Profile 14 (OHIP-14). Testing of the outcomes was carried out at 1, 2, 3, 4 and 6 days after treatment for VAS and at 6 days for OHIP-14.

Results

For pain scores, the test group showed a significant decrease by time, this was evident from day 1 (P < .001); at day 3, median and interquartile range (IQR) values were 0 (0-1). For the placebo group, a non-significant change by time was reported between baseline and day 1; at day 3, the median value was 7 (IQR, 7-9). The test group showed significantly lower mean duration than the placebo group (P < .001). OHIP-14 scores after 6 days showed that the test group had a significantly lower score than placebo (P < .001).

Conclusions

Topical insulin-liposomal formulation showed marked effectiveness in management of aphthous ulcers.

目的评价胰岛素脂质体凝胶外用治疗阿弗他溃疡的疗效。方法80名患有轻微阿弗他溃疡的参与者被随机分为外用胰岛素脂质体凝胶或安慰剂凝胶（每天一次），持续6天。结果评估包括疼痛的视觉模拟评分（VAS）（主要结果），次要结果包括溃疡持续时间和使用口腔健康影响档案14（OHIP-14）治疗对生活质量的影响。在VAS治疗后1、2、3、4和6天以及OHIP-14治疗后6天对结果进行测试。结果对于疼痛评分，测试组显示出随时间的显著下降，这从第1天开始就很明显（P<.001）；在第3天，中位数和四分位间距（IQR）值为0（0-1）。对于安慰剂组，在基线和第1天之间报告了随时间的无显著变化；在第3天，中位数为7（IQR，7-9）。试验组的平均持续时间明显低于安慰剂组（P＜0.001）。6天后OHIP-14评分显示试验组的评分明显低于安慰剂（P＜.001）。结论：口服胰岛素脂质体制剂在治疗阿弗他溃疡方面表现出显著的有效性。

{"title":"Topical insulin-liposomal formulation in management of recurrent aphthous ulcers: A randomized placebo-controlled trial","authors":"Naglaa M. El-Wakeel, Nadia M. Morsi, Dalia M. Ghorab, Ghada E. Yassin, Marwa H. S. Dawoud","doi":"10.1111/jicd.12437","DOIUrl":"10.1111/jicd.12437","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Aim</h3>\u0000 \u0000 <p>To evaluate the effectiveness of topical insulin-liposomal gel in aphthous ulcer treatment.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>80 participants with minor aphthous ulcers were randomly divided to receive either topical insulin-liposomal gel or placebo gel (once daily) for 6 days. Assessment of outcomes included visual analog scale (VAS) for pain (primary outcome), and secondary outcomes included ulcer duration and impact of treatment on quality of life using the Oral Health Impact Profile 14 (OHIP-14). Testing of the outcomes was carried out at 1, 2, 3, 4 and 6 days after treatment for VAS and at 6 days for OHIP-14.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>For pain scores, the test group showed a significant decrease by time, this was evident from day 1 (<i>P</i> < .001); at day 3, median and interquartile range (IQR) values were 0 (0-1). For the placebo group, a non-significant change by time was reported between baseline and day 1; at day 3, the median value was 7 (IQR, 7-9). The test group showed significantly lower mean duration than the placebo group (<i>P</i> < .001). OHIP-14 scores after 6 days showed that the test group had a significantly lower score than placebo (<i>P</i> < .001).</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>Topical insulin-liposomal formulation showed marked effectiveness in management of aphthous ulcers.</p>\u0000 </section>\u0000 </div>","PeriodicalId":16204,"journal":{"name":"Journal of Investigative and Clinical Dentistry","volume":"10 4","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-07-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/jicd.12437","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41886850","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 6

Epidemiological profile of young patients with squamous cell carcinoma in northeast Brazil 巴西东北部年轻鳞状细胞癌患者的流行病学分析。

Q1 Medicine

Journal of Investigative and Clinical Dentistry

Pub Date : 2019-07-27 DOI: 10.1111/jicd.12436

Karen Rayssa Borba Ribeiro, Cledinaldo Lira Júnior, Sandra Aparecida Marinho, Sérgio Henrique Gonçalves de Carvalho, Gustavo Gomes Agripino, Dmitry José de Santana Sarmento

Aim

The aim of the present study was to delineate the epidemiological profile of young patients with squamous cell carcinoma (SCC) treated at a healthcare service in northeastern Brazil.

Methods

A cross-sectional study was performed involving the quantitative analysis of 51 medical records from patients diagnosed with SCC between 1998 and 2013. All patients aged ≤50 years were included in the study. Statistical analysis involved Pearson's χ²-test, Fisher's exact test, and the Mann-Whitney U-test, with the level of significance set at 5% (P < .05).

Results

The mean age was 46 ± 7.94 years, and males accounted for 80.4% of the sample. The tongue was the most affected site (37.3%). Most tumors were classified as stage III (26.3%) or IV (42.1%). Surgery associated with chemotherapy was the most common therapeutic approach (39.3%). Female patients had a lower mean age (P = .013) and less advanced clinical staging (P = .022). Smoking was more associated with male sex (P = .043).

Conclusion

SCC in young patients is more common in males aged 46 ± 7.94 years, with the tongue being the most affected anatomic site. Important differences were found in clinical-epidemiological features between young men and women.

目的本研究的目的是描述在巴西东北部医疗服务机构接受治疗的年轻鳞状细胞癌（SCC）患者的流行病学特征。方法进行横断面研究，对1998年至2013年间诊断为鳞状细胞癌的51份病历进行定量分析。所有年龄≤50岁的患者均纳入研究。统计分析包括Pearsonχ2检验、Fisher精确检验和Mann-Whitney U检验，显著性水平为5%（P＜0.05）。结果平均年龄为46±7.94岁，男性占样本的80.4%。舌头是受影响最严重的部位（37.3%）。大多数肿瘤分为III期（26.3%）或IV期（42.1%）。与化疗相关的手术是最常见的治疗方法（39.3%）。女性患者的平均年龄较低（P=0.013），临床分期较低（P=.022）。吸烟与男性更相关（P=.043）。结论年轻患者的SCC更常见年龄46±7.94岁的男性，其中舌头是受影响最大的解剖部位。年轻男性和女性在临床流行病学特征方面存在重要差异。

{"title":"Epidemiological profile of young patients with squamous cell carcinoma in northeast Brazil","authors":"Karen Rayssa Borba Ribeiro, Cledinaldo Lira Júnior, Sandra Aparecida Marinho, Sérgio Henrique Gonçalves de Carvalho, Gustavo Gomes Agripino, Dmitry José de Santana Sarmento","doi":"10.1111/jicd.12436","DOIUrl":"10.1111/jicd.12436","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Aim</h3>\u0000 \u0000 <p>The aim of the present study was to delineate the epidemiological profile of young patients with squamous cell carcinoma (SCC) treated at a healthcare service in northeastern Brazil.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>A cross-sectional study was performed involving the quantitative analysis of 51 medical records from patients diagnosed with SCC between 1998 and 2013. All patients aged ≤50 years were included in the study. Statistical analysis involved Pearson's χ<sup>2</sup>-test, Fisher's exact test, and the Mann-Whitney <i>U</i>-test, with the level of significance set at 5% (<i>P </i>< .05).</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>The mean age was 46 ± 7.94 years, and males accounted for 80.4% of the sample. The tongue was the most affected site (37.3%). Most tumors were classified as stage III (26.3%) or IV (42.1%). Surgery associated with chemotherapy was the most common therapeutic approach (39.3%). Female patients had a lower mean age (<i>P </i>= .013) and less advanced clinical staging (<i>P </i>= .022). Smoking was more associated with male sex (<i>P </i>= .043).</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>SCC in young patients is more common in males aged 46 ± 7.94 years, with the tongue being the most affected anatomic site. Important differences were found in clinical-epidemiological features between young men and women.</p>\u0000 </section>\u0000 </div>","PeriodicalId":16204,"journal":{"name":"Journal of Investigative and Clinical Dentistry","volume":"10 4","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-07-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/jicd.12436","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44113918","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

Shear bond strengths of calcium silicate-based sealer to dentin and calcium silicate-impregnated gutta-percha 硅酸钙基封口剂与牙本质和硅酸钙浸渍胶的剪切结合强度。

Q1 Medicine

Journal of Investigative and Clinical Dentistry

Pub Date : 2019-07-27 DOI: 10.1111/jicd.12444

Nonthicha Banphakarn, Kallaya Yanpiset, Danuchit Banomyong

Aim

To compare the shear bond strengths of a calcium silicate-based root canal sealer (CSS), to dentin or calcium silicate-impregnated gutta-percha (CSGP), with the shear bond strengths of an epoxy resin-based sealer.

Methods

For sealer/dentin testing, 40 hemisectioned roots were divided into 2 groups. Coronal root dentin discs were bonded with CSS or epoxy resin-based sealer. For sealer/core material testing, CSGP and conventional gutta-percha discs were bonded with a matching sealer (N = 20 per group). The shear bond strengths were tested using a universal testing machine. The failed modes were analyzed using stereomicroscopy and scanning electron microscopy.

Results

The CSS sealer had significantly higher shear bond strength to dentin than did epoxy resin-based sealer (P < .01). However, shear bond strengths of the 2 sealers to core materials did not significantly differ (P = .74). The remnants of the CSS sealer adhering to dentin or calcium silicate gutta-percha disc were observed.

Conclusions

The CSS sealer had better adhesion to dentin than did epoxy resin-based sealer. However, the bond of the CSS sealer to impregnated gutta-percha was not superior to the epoxy resin-based sealer bonded to conventional gutta-percha.

目的比较硅酸钙基根管封闭剂（CSS）、牙本质或硅酸钙浸渍的牙胶（CSGP）与环氧树脂基封闭剂的剪切结合强度。方法将40根半切根分为2组进行封闭/牙本质检测。冠根牙本质盘采用CSS或环氧树脂基密封材料粘接。对于密封剂/核心材料测试，用匹配的密封剂（每组N=20）粘合CSGP和传统的牙胶盘。剪切结合强度使用通用测试机进行测试。使用立体显微镜和扫描电子显微镜分析失效模式。结果CSS密封胶与牙本质的剪切结合强度明显高于环氧树脂基密封胶（P＜0.01）。然而，两种密封胶与核心材料的剪切粘合强度没有显著差异（P＝.74）。观察到CSS密封胶残留在牙本质或硅酸钙牙胶盘上。结论CSS基牙本质封闭剂与环氧树脂基牙本质密封剂相比具有更好的粘接性能。然而，CSS密封剂与浸渍的牙胶的结合并不优于与传统牙胶结合的环氧树脂基密封剂。

{"title":"Shear bond strengths of calcium silicate-based sealer to dentin and calcium silicate-impregnated gutta-percha","authors":"Nonthicha Banphakarn, Kallaya Yanpiset, Danuchit Banomyong","doi":"10.1111/jicd.12444","DOIUrl":"10.1111/jicd.12444","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Aim</h3>\u0000 \u0000 <p>To compare the shear bond strengths of a calcium silicate-based root canal sealer (CSS), to dentin or calcium silicate-impregnated gutta-percha (CSGP), with the shear bond strengths of an epoxy resin-based sealer.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>For sealer/dentin testing, 40 hemisectioned roots were divided into 2 groups. Coronal root dentin discs were bonded with CSS or epoxy resin-based sealer. For sealer/core material testing, CSGP and conventional gutta-percha discs were bonded with a matching sealer (N = 20 per group). The shear bond strengths were tested using a universal testing machine. The failed modes were analyzed using stereomicroscopy and scanning electron microscopy.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>The CSS sealer had significantly higher shear bond strength to dentin than did epoxy resin-based sealer (<i>P</i> < .01). However, shear bond strengths of the 2 sealers to core materials did not significantly differ (<i>P</i> = .74). The remnants of the CSS sealer adhering to dentin or calcium silicate gutta-percha disc were observed.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>The CSS sealer had better adhesion to dentin than did epoxy resin-based sealer. However, the bond of the CSS sealer to impregnated gutta-percha was not superior to the epoxy resin-based sealer bonded to conventional gutta-percha.</p>\u0000 </section>\u0000 </div>","PeriodicalId":16204,"journal":{"name":"Journal of Investigative and Clinical Dentistry","volume":"10 4","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-07-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/jicd.12444","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44950391","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Changes in oral microbial profiles associated with oral squamous cell carcinoma vs leukoplakia 口腔鳞状细胞癌与白斑相关的口腔微生物谱变化。

Q1 Medicine

Journal of Investigative and Clinical Dentistry

Pub Date : 2019-07-24 DOI: 10.1111/jicd.12445

Kengo Hashimoto, Dai Shimizu, Sho Hirabayashi, Sei Ueda, Satoru Miyabe, Ichiro Oh-iwa, Toru Nagao, Kazuo Shimozato, Shuji Nomoto

Aim

Oral squamous cell carcinoma (OSCC) is the most frequently occurring cancer among head and neck SCC worldwide. The identification of novel effective biomarkers for early detection may greatly improve the survival rate and prognosis of patients with OSCC. This study aimed to identify specific oral microbial profiles associated with OSCC.

Methods

Saliva samples were collected from oral leukoplakia (OLK) and OSCC patients (N = 6 each) and healthy controls (HC; N = 4). Total bacterial genomic DNA was isolated and 16S rRNA gene survey was performed by next-generation sequencing of the V4 region. The relative distribution of abundance for phylogenetic groups was compared among the OSCC and OLK groups.

Results

The 448 operational taxonomic units detected from the libraries were classified into 133 genera, 69 families, 41 orders, 26 classes and 12 phyla. The abundance of phyla Bacteroidetes and genus Solobacterium was notably higher in the OSCC group when compared with the OLK group, whereas those of genus Streptococcus was significantly lower in the OSCC group when compared with the OLK.

Conclusion

These changes in the salivary microbiome may have potential applications as a novel diagnostic tool for the early detection of OSCC.

目的口腔鳞状细胞癌（OSCC）是全世界头颈部鳞状细胞癌中最常见的癌症。鉴定用于早期检测的新型有效生物标志物可能大大提高OSCC患者的生存率和预后。本研究旨在确定与OSCC相关的特定口腔微生物特征。方法从口腔白斑（OLK）和OSCC患者（各N=6）和健康对照组（HC；N=4）采集唾液样本。分离总细菌基因组DNA，并通过V4区域的下一代测序进行16S rRNA基因调查。比较了OSCC和OLK组之间系统发育组丰度的相对分布。结果从图书馆中检索到448个操作分类单元，分为12门、26纲、41目、69科、133属。与OLK组相比，OSCC组中拟杆菌门和梭杆菌属的丰度显著较高，而与OLK相比，OSCC组中链球菌属的丰度明显较低。结论唾液微生物组的这些变化可能作为早期检测OSCC的新诊断工具具有潜在的应用前景。

{"title":"Changes in oral microbial profiles associated with oral squamous cell carcinoma vs leukoplakia","authors":"Kengo Hashimoto, Dai Shimizu, Sho Hirabayashi, Sei Ueda, Satoru Miyabe, Ichiro Oh-iwa, Toru Nagao, Kazuo Shimozato, Shuji Nomoto","doi":"10.1111/jicd.12445","DOIUrl":"10.1111/jicd.12445","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Aim</h3>\u0000 \u0000 <p>Oral squamous cell carcinoma (OSCC) is the most frequently occurring cancer among head and neck SCC worldwide. The identification of novel effective biomarkers for early detection may greatly improve the survival rate and prognosis of patients with OSCC. This study aimed to identify specific oral microbial profiles associated with OSCC.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>Saliva samples were collected from oral leukoplakia (OLK) and OSCC patients (N = 6 each) and healthy controls (HC; N = 4). Total bacterial genomic DNA was isolated and 16S rRNA gene survey was performed by next-generation sequencing of the V4 region. The relative distribution of abundance for phylogenetic groups was compared among the OSCC and OLK groups.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>The 448 operational taxonomic units detected from the libraries were classified into 133 genera, 69 families, 41 orders, 26 classes and 12 phyla. The abundance of phyla Bacteroidetes and genus <i>Solobacterium</i> was notably higher in the OSCC group when compared with the OLK group, whereas those of genus <i>Streptococcus</i> was significantly lower in the OSCC group when compared with the OLK.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>These changes in the salivary microbiome may have potential applications as a novel diagnostic tool for the early detection of OSCC.</p>\u0000 </section>\u0000 </div>","PeriodicalId":16204,"journal":{"name":"Journal of Investigative and Clinical Dentistry","volume":"10 4","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-07-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/jicd.12445","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41606974","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 21