首页 > 最新文献

Journal of Life Science最新文献

英文 中文
Inhibitory Effect of Ethanol Extract of Monascus-fermented Red Yeast Rice on Proinflammatory iNOS and COX-2 Protein Expression in LPS-stimulated RAW 264.7 Macrophage Cells 红曲米乙醇提取物对lps刺激的RAW 264.7巨噬细胞促炎iNOS和COX-2蛋白表达的抑制作用
Pub Date : 2020-01-01 DOI: 10.5352/JLS.2020.30.4.352
Ki-Hyun Kim, Jung-Hyeong Lee, G. Kwon, E. Seo, Jung-Bok Lee
{"title":"Inhibitory Effect of Ethanol Extract of Monascus-fermented Red Yeast Rice on Proinflammatory iNOS and COX-2 Protein Expression in LPS-stimulated RAW 264.7 Macrophage Cells","authors":"Ki-Hyun Kim, Jung-Hyeong Lee, G. Kwon, E. Seo, Jung-Bok Lee","doi":"10.5352/JLS.2020.30.4.352","DOIUrl":"https://doi.org/10.5352/JLS.2020.30.4.352","url":null,"abstract":"","PeriodicalId":16322,"journal":{"name":"Journal of Life Science","volume":"1997 1","pages":"352-358"},"PeriodicalIF":0.0,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78611390","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
Regulation of Abiotic Stress Response by Alternative Splicing in Plants 植物选择性剪接对非生物胁迫响应的调控
Pub Date : 2020-01-01 DOI: 10.5352/JLS.2020.30.6.570
Hye-Yeon Seok, S. Lee, Y. Moon
{"title":"Regulation of Abiotic Stress Response by Alternative Splicing in Plants","authors":"Hye-Yeon Seok, S. Lee, Y. Moon","doi":"10.5352/JLS.2020.30.6.570","DOIUrl":"https://doi.org/10.5352/JLS.2020.30.6.570","url":null,"abstract":"","PeriodicalId":16322,"journal":{"name":"Journal of Life Science","volume":"3 1-2 1","pages":"570-579"},"PeriodicalIF":0.0,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85356892","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
중선포천, 관곡천, 광양서천에서 염생식물과 수생식물상 분포 中仙浦川,冠谷川,光阳舒川的盐生植物和水生植物分布
Pub Date : 2020-01-01 DOI: 10.5352/JLS.2020.30.2.186
M. Huh
{"title":"중선포천, 관곡천, 광양서천에서 염생식물과 수생식물상 분포","authors":"M. Huh","doi":"10.5352/JLS.2020.30.2.186","DOIUrl":"https://doi.org/10.5352/JLS.2020.30.2.186","url":null,"abstract":"","PeriodicalId":16322,"journal":{"name":"Journal of Life Science","volume":"36 1","pages":"186-190"},"PeriodicalIF":0.0,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76870823","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Inhibitory Effects of Tenebrio molitor Larvae Ethanol Extract on RANKL-Induced Osteoclast Differentiation 黄粉虫幼虫乙醇提取物对rankl诱导的破骨细胞分化的抑制作用
Pub Date : 2020-01-01 DOI: 10.5352/JLS.2020.30.11.983
M. Seo, Minhee Baek, H. Lee, Yong pyo Shin, Joon-Ha Lee, In-woo Kim, Mi-Ae Kim, Jae‐Sam Hwang
The balance between bone-resorbing osteoclasts and bone-forming osteoblasts is key to bone health. An imbalance between osteoclasts and osteoblasts leads to various bone-related disorders, such as osteoporosis, osteomalacia, and osteopetrosis. However, the bone-resorption inhibitor drugs that are currently used may cause side effects. Natural substances have recently received much attention as therapeutic drugs for the treatment of bone health. This study was designed to determine the effect of Tenebrio molitor larvae ethanol extract (TME) on receptor activator of nuclear factor-κB ligand (RANKL)induced osteoclast differentiation. To measure the effect of TME on osteoclast differentiation, RAW264.7 cells were treated with RANKL with or without TME for 5 days. The tartrate-resistant acid phosphatase (TRAP) activity was significantly inhibited by treatment of TME without cytotoxicity up to 2 mg/ ml. In addition, TME effectively suppressed expression of osteoclast differentiation-related marker genes and proteins such as TRAP, NFATc1, and c-Src. TME also significantly inhibited the p38 mitogen-activated protein kinase (MAPK) signaling pathway without affecting ERK and JNK signaling in RANKL-induced RAW264.7 cells. Consequently, we conclude that TME suppresses osteoclast differentiation by inhibiting RANKL-induced osteoclastogenic genes expression through the p38 MAPK signaling pathways. These results suggest that TME and its bioactive components are potential therapeutics for bone-related diseases such as osteoporosis.
骨吸收破骨细胞和成骨细胞之间的平衡是骨骼健康的关键。破骨细胞和成骨细胞之间的不平衡导致各种骨相关疾病,如骨质疏松症、骨软化症和骨质疏松症。然而,目前使用的骨吸收抑制剂药物可能会产生副作用。近年来,天然物质作为治疗骨骼健康的药物受到了广泛的关注。本实验旨在探讨黄粉虫幼虫乙醇提取物(TME)对核因子-κB配体受体激活剂(RANKL)诱导的破骨细胞分化的影响。为了检测TME对破骨细胞分化的影响,我们分别用RANKL治疗RAW264.7细胞5天。2 mg/ ml无细胞毒性的TME显著抑制了抗酒石酸酸性磷酸酶(TRAP)活性。此外,TME有效抑制了破骨细胞分化相关标记基因和蛋白的表达,如TRAP、NFATc1和c-Src。在rankl诱导的RAW264.7细胞中,TME还显著抑制p38丝裂原活化蛋白激酶(MAPK)信号通路,而不影响ERK和JNK信号通路。因此,我们得出结论,TME通过p38 MAPK信号通路抑制rankl诱导的破骨细胞基因表达,从而抑制破骨细胞分化。这些结果表明,TME及其生物活性成分是治疗骨质疏松症等骨相关疾病的潜在药物。
{"title":"Inhibitory Effects of Tenebrio molitor Larvae Ethanol Extract on RANKL-Induced Osteoclast Differentiation","authors":"M. Seo, Minhee Baek, H. Lee, Yong pyo Shin, Joon-Ha Lee, In-woo Kim, Mi-Ae Kim, Jae‐Sam Hwang","doi":"10.5352/JLS.2020.30.11.983","DOIUrl":"https://doi.org/10.5352/JLS.2020.30.11.983","url":null,"abstract":"The balance between bone-resorbing osteoclasts and bone-forming osteoblasts is key to bone health. An imbalance between osteoclasts and osteoblasts leads to various bone-related disorders, such as osteoporosis, osteomalacia, and osteopetrosis. However, the bone-resorption inhibitor drugs that are currently used may cause side effects. Natural substances have recently received much attention as therapeutic drugs for the treatment of bone health. This study was designed to determine the effect of Tenebrio molitor larvae ethanol extract (TME) on receptor activator of nuclear factor-κB ligand (RANKL)induced osteoclast differentiation. To measure the effect of TME on osteoclast differentiation, RAW264.7 cells were treated with RANKL with or without TME for 5 days. The tartrate-resistant acid phosphatase (TRAP) activity was significantly inhibited by treatment of TME without cytotoxicity up to 2 mg/ ml. In addition, TME effectively suppressed expression of osteoclast differentiation-related marker genes and proteins such as TRAP, NFATc1, and c-Src. TME also significantly inhibited the p38 mitogen-activated protein kinase (MAPK) signaling pathway without affecting ERK and JNK signaling in RANKL-induced RAW264.7 cells. Consequently, we conclude that TME suppresses osteoclast differentiation by inhibiting RANKL-induced osteoclastogenic genes expression through the p38 MAPK signaling pathways. These results suggest that TME and its bioactive components are potential therapeutics for bone-related diseases such as osteoporosis.","PeriodicalId":16322,"journal":{"name":"Journal of Life Science","volume":"226 1","pages":"983-989"},"PeriodicalIF":0.0,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78473974","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 2
Recent Trends on Telomerase Activators, Sirtuin Activators, and Senolytics as a Potential Anti-aging Agent 端粒酶激活剂、Sirtuin激活剂和抗衰老药物的研究进展
Pub Date : 2020-01-01 DOI: 10.5352/JLS.2020.30.9.819
Moon‐Moo Kim
All living organisms exhibit the characteristics of aging, such as skin wrinkle formation, muscle degeneration, cataracts, and hair graying as the number of aged cells increases over time. Senescence, which is known as a key cause of aging, is directly related to the aging of living organisms because cells are aged by external and internal factors and eventually cell proliferation is stopped. Senescence is caused by the gradual shortening of the telomere with cell division, and lifespan is determined by the length of the telomere. Recently, it has been found that the histone deacetylase, which can influence gene expression, is not only involved in yeast but also deeply involved in anti-aging mechanisms in both C. elegans and humans. It was also discovered that old cells play a decisive role in the aging phenomenon, and it has been reported that it is possible to promote the proliferation of young cells and delay aging by removing these senescent cells from the inside. Therefore, in order to develop potential anti-aging agents in the future, research should begin with an in-depth study of telomerase activators, sirtuin activators, and senolytics.
所有生物体都表现出衰老的特征,如皮肤皱纹的形成、肌肉退化、白内障和头发变白,因为衰老细胞的数量随着时间的推移而增加。被称为“衰老的主要原因”的衰老与生物体的衰老直接相关,因为细胞在外部和内部因素的作用下衰老,最终细胞增殖停止。衰老是由细胞分裂时端粒逐渐缩短引起的,而寿命是由端粒的长度决定的。近年来,研究发现,影响基因表达的组蛋白去乙酰化酶不仅参与酵母,而且深度参与秀丽隐杆线虫和人类的抗衰老机制。人们还发现,衰老细胞在衰老现象中起着决定性的作用,有报道称,通过从内部清除这些衰老细胞,可以促进年轻细胞的增殖,延缓衰老。因此,为了在未来开发潜在的抗衰老药物,研究应从深入研究端粒酶激活剂、sirtuin激活剂和抗衰老剂开始。
{"title":"Recent Trends on Telomerase Activators, Sirtuin Activators, and Senolytics as a Potential Anti-aging Agent","authors":"Moon‐Moo Kim","doi":"10.5352/JLS.2020.30.9.819","DOIUrl":"https://doi.org/10.5352/JLS.2020.30.9.819","url":null,"abstract":"All living organisms exhibit the characteristics of aging, such as skin wrinkle formation, muscle degeneration, cataracts, and hair graying as the number of aged cells increases over time. Senescence, which is known as a key cause of aging, is directly related to the aging of living organisms because cells are aged by external and internal factors and eventually cell proliferation is stopped. Senescence is caused by the gradual shortening of the telomere with cell division, and lifespan is determined by the length of the telomere. Recently, it has been found that the histone deacetylase, which can influence gene expression, is not only involved in yeast but also deeply involved in anti-aging mechanisms in both C. elegans and humans. It was also discovered that old cells play a decisive role in the aging phenomenon, and it has been reported that it is possible to promote the proliferation of young cells and delay aging by removing these senescent cells from the inside. Therefore, in order to develop potential anti-aging agents in the future, research should begin with an in-depth study of telomerase activators, sirtuin activators, and senolytics.","PeriodicalId":16322,"journal":{"name":"Journal of Life Science","volume":"23 1","pages":"819-825"},"PeriodicalIF":0.0,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77986412","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Recent Advances in Understanding the Mechanisms of Particulate Matter-mediated Ocular Diseases 颗粒物质介导的眼部疾病机制研究进展
Pub Date : 2020-01-01 DOI: 10.5352/JLS.2020.30.8.722
Hyesook Lee, Yung-Hyun Choi
As one of the most serious health risk factors, air pollution can no longer be ignored. Particulate matter (PM) is an important and harmful component of air pollution that originates from a variety of sources. Numerous recent studies have linked PM to a range of conditions including cancer, cardiovascular, respiratory, and skin disease. The eye, despite being directly exposed to air pollution, has been investigated in very few of these studies. In this review, we describe the evidence from in vitro and in vivo studies, as well as epidemiological investigations, that supports the association between exposure to PM and the development of ocular conditions such as surface and retinal disease and glaucoma. Based on the results of previous studies, we suggest that PM exposure can lead to oxidative stress, inflammation, autophagy, and, ultimately, ocular surface disease. Nevertheless, almost no studies focus on ocular surface damage from PM while some epidemiological and clinical studies report on the posterior of the eye. However, the underlying pathological mechanisms in the posterior following PM exposure have yet to be identified, and further studies are therefore warranted of the ocular surface as well as the posterior part of the eye.
空气污染作为最严重的健康危害因素之一,已不容忽视。颗粒物(PM)是空气污染的重要有害成分,其来源多种多样。最近的许多研究都将PM与一系列疾病联系起来,包括癌症、心血管疾病、呼吸系统疾病和皮肤病。尽管眼睛直接暴露在空气污染中,但这些研究中很少对眼睛进行调查。在这篇综述中,我们描述了来自体外和体内研究以及流行病学调查的证据,这些证据支持PM暴露与眼部疾病(如表面和视网膜疾病以及青光眼)的发展之间的关联。基于以往的研究结果,我们认为PM暴露可导致氧化应激、炎症、自噬,并最终导致眼表疾病。然而,几乎没有研究关注PM引起的眼表损伤,而一些流行病学和临床研究报道了眼后部。然而,PM暴露后后部的潜在病理机制尚未确定,因此需要对眼表和眼后部进行进一步的研究。
{"title":"Recent Advances in Understanding the Mechanisms of Particulate Matter-mediated Ocular Diseases","authors":"Hyesook Lee, Yung-Hyun Choi","doi":"10.5352/JLS.2020.30.8.722","DOIUrl":"https://doi.org/10.5352/JLS.2020.30.8.722","url":null,"abstract":"As one of the most serious health risk factors, air pollution can no longer be ignored. Particulate matter (PM) is an important and harmful component of air pollution that originates from a variety of sources. Numerous recent studies have linked PM to a range of conditions including cancer, cardiovascular, respiratory, and skin disease. The eye, despite being directly exposed to air pollution, has been investigated in very few of these studies. In this review, we describe the evidence from in vitro and in vivo studies, as well as epidemiological investigations, that supports the association between exposure to PM and the development of ocular conditions such as surface and retinal disease and glaucoma. Based on the results of previous studies, we suggest that PM exposure can lead to oxidative stress, inflammation, autophagy, and, ultimately, ocular surface disease. Nevertheless, almost no studies focus on ocular surface damage from PM while some epidemiological and clinical studies report on the posterior of the eye. However, the underlying pathological mechanisms in the posterior following PM exposure have yet to be identified, and further studies are therefore warranted of the ocular surface as well as the posterior part of the eye.","PeriodicalId":16322,"journal":{"name":"Journal of Life Science","volume":"73 1","pages":"722-730"},"PeriodicalIF":0.0,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88685562","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
Heterosigma akashiwo를 모니터하기 위한 뉴클레아제 보호 분석이 통합된 샌드위치 혼성(NPA-SH)의 개발 一种结合了磷酸酶保护分析来监测Heterosigma akashiwo的三明治混合(NPA-SH)。
Pub Date : 2020-01-01 DOI: 10.5352/JLS.2020.30.1.26
Mingyeong Kang, Mi-Ryong Park, K. Kim, Taek Kyun Lee
{"title":"Heterosigma akashiwo를 모니터하기 위한 뉴클레아제 보호 분석이 통합된 샌드위치 혼성(NPA-SH)의 개발","authors":"Mingyeong Kang, Mi-Ryong Park, K. Kim, Taek Kyun Lee","doi":"10.5352/JLS.2020.30.1.26","DOIUrl":"https://doi.org/10.5352/JLS.2020.30.1.26","url":null,"abstract":"","PeriodicalId":16322,"journal":{"name":"Journal of Life Science","volume":"86 1","pages":"26-31"},"PeriodicalIF":0.0,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90513835","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Renal Failure in a Female Muskrat 雌性麝鼠的肾功能衰竭
Pub Date : 2020-01-01 DOI: 10.5352/JLS.2020.30.7.630
Ullah H. M. Arif, A. Elfadl, Park, Sun-young, Chung Myung-Jin, Son Ji-Yoon, Yun Hyun-Ho, Park, Jae-min, Yim Jae-Hyuk, Jung Seung-Jun, Park Jinkyu, Jeong Kyu-Shik
Renal failure syndrome in wild mammals is infrequently reported. Muskrat (Ondatra zibethicus) is a medium-sized rodent known to carry many diseases but rarely exhibiting renal failure. A six-month old female muskrat was submitted to our laboratory for pathological diagnosis, and necropsy revealed severe renal damage with sand-like lithiasis in the ureter, renal calculi, and hydronephrosis. All major organs, including the cerebrum, also showed systemic hemorrhage and calcification which may have been due to uremia induced by renal failure. Histopathologically, necrosis and microcalcification were detected in the renal cortex and the medulla, especially in the proximal convoluted tubules and collecting ducts of the kidney. Significant hyalinization of the glomeruli was also observed, and this suggested chronic nephritis. These findings would support mycotoxic effects, particularly on the kidney. Moreover, infiltration of neutrophils and mononuclear cells was observed in the lung and of plasma cells in the spleen. The definitive cause of the toxic effects in this case of muskrat renal failure could be attributed to contaminated food.
野生哺乳动物肾功能衰竭综合征的报道并不多见。麝鼠(Ondatra zibethicus)是一种中等大小的啮齿动物,已知携带许多疾病,但很少表现出肾功能衰竭。我们将一只6个月大的雌性麝鼠送到我们的实验室进行病理诊断,尸检发现严重的肾脏损害,包括输尿管沙样结石、肾结石和肾积水。所有主要器官,包括大脑,也显示全身性出血和钙化,这可能是由于肾功能衰竭引起的尿毒症。病理组织学上,肾皮质和肾髓质可见坏死和微钙化,尤其是近曲小管和肾集管。肾小球明显透明化,提示慢性肾炎。这些发现将支持真菌毒性作用,特别是对肾脏的作用。肺内可见中性粒细胞和单核细胞浸润,脾内可见浆细胞浸润。在这个麝鼠肾衰竭的案例中,毒性作用的最终原因可能是受污染的食物。
{"title":"Renal Failure in a Female Muskrat","authors":"Ullah H. M. Arif, A. Elfadl, Park, Sun-young, Chung Myung-Jin, Son Ji-Yoon, Yun Hyun-Ho, Park, Jae-min, Yim Jae-Hyuk, Jung Seung-Jun, Park Jinkyu, Jeong Kyu-Shik","doi":"10.5352/JLS.2020.30.7.630","DOIUrl":"https://doi.org/10.5352/JLS.2020.30.7.630","url":null,"abstract":"Renal failure syndrome in wild mammals is infrequently reported. Muskrat (Ondatra zibethicus) is a medium-sized rodent known to carry many diseases but rarely exhibiting renal failure. A six-month old female muskrat was submitted to our laboratory for pathological diagnosis, and necropsy revealed severe renal damage with sand-like lithiasis in the ureter, renal calculi, and hydronephrosis. All major organs, including the cerebrum, also showed systemic hemorrhage and calcification which may have been due to uremia induced by renal failure. Histopathologically, necrosis and microcalcification were detected in the renal cortex and the medulla, especially in the proximal convoluted tubules and collecting ducts of the kidney. Significant hyalinization of the glomeruli was also observed, and this suggested chronic nephritis. These findings would support mycotoxic effects, particularly on the kidney. Moreover, infiltration of neutrophils and mononuclear cells was observed in the lung and of plasma cells in the spleen. The definitive cause of the toxic effects in this case of muskrat renal failure could be attributed to contaminated food.","PeriodicalId":16322,"journal":{"name":"Journal of Life Science","volume":"124 1","pages":"630-633"},"PeriodicalIF":0.0,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91297199","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
The Development of a New Soybean Strain Without Kunitz Trypsin Inhibitor, Lectin, and 7S α' Subunit Protein 不含库尼茨胰蛋白酶抑制剂、凝集素和7S α亚基蛋白的大豆新品系的选育
Pub Date : 2020-01-01 DOI: 10.5352/JLS.2020.30.7.592
Won Gi Chae, S. Choi, Gyung Young Kang, J. Chung
Soybean [Glycine max (L.) Merr.] seeds contain an average of 40% protein on a dry weight basis, but they also contain antinutritional elements such as lectin, Kunitz trypsin inhibitor (KTI), and 7S α'subunit protein. The objective of this research was to develop a new soybean genotype with triple recessive alleles for these elements. Three parents (Gaechuck#2, PI506876, and Le-16) were used to develop the genetic population, and the presence of lectin and KTI ᅦprotein was detected using Western blot while 7S α' subunit protein was detected using SDS-PAGE. One F3 plant strain with proper agronomical traits such as type, height, seed quality, and 100-seed weight was selected. The genotype of the developed strain is titilelecgy1cgy1, that is KTI, lectin, and 7S α' subunit protein free. The new strain has a purple flower, determinate growth habit, and light yellow pods at maturity. The seed has a buffer hilum and is yellow in color. The new strain’s height was 58 cm compared to the Daewonkong cultivar at 46 cm, and its 100-seed weight was 27.1 g, smaller than the Daewonkong at 29.0 g. This is the first new soybean strain with the titilelecgy1cgy1 genotype, and it can be used to improve yellow soybean cultivars of high quality and function.
大豆[甘氨酸max (L.)]稳定。在干重基础上平均含有40%的蛋白质,但它们也含有抗营养元素,如凝集素、库尼茨胰蛋白酶抑制剂(KTI)和7S α'亚基蛋白。本研究的目的是开发一个具有这些元素的三隐性等位基因的大豆新基因型。采用Gaechuck#2、PI506876和Le-16亲本构建遗传群体,Western blot检测凝集素和KTIᅦ蛋白的存在,SDS-PAGE检测7S α’亚基蛋白的存在。选择了一株类型、株高、种子品质、百粒重等农艺性状适宜的F3株系。该菌株基因型为titilelecgy1cgy1,即不含KTI、凝集素和7S α’亚基蛋白。新品系花紫色,生长习性确定,成熟时荚果淡黄色。种子有一个缓冲门,颜色为黄色。新品系的株高为58 cm,比大原空品种(46 cm)高,百粒重为27.1 g,比大原空品种(29.0 g)小。这是首个具有titilelecgy1cgy1基因型的大豆新品系,可用于黄豆优质功能品种的改良。
{"title":"The Development of a New Soybean Strain Without Kunitz Trypsin Inhibitor, Lectin, and 7S α' Subunit Protein","authors":"Won Gi Chae, S. Choi, Gyung Young Kang, J. Chung","doi":"10.5352/JLS.2020.30.7.592","DOIUrl":"https://doi.org/10.5352/JLS.2020.30.7.592","url":null,"abstract":"Soybean [Glycine max (L.) Merr.] seeds contain an average of 40% protein on a dry weight basis, but they also contain antinutritional elements such as lectin, Kunitz trypsin inhibitor (KTI), and 7S α'subunit protein. The objective of this research was to develop a new soybean genotype with triple recessive alleles for these elements. Three parents (Gaechuck#2, PI506876, and Le-16) were used to develop the genetic population, and the presence of lectin and KTI ᅦprotein was detected using Western blot while 7S α' subunit protein was detected using SDS-PAGE. One F3 plant strain with proper agronomical traits such as type, height, seed quality, and 100-seed weight was selected. The genotype of the developed strain is titilelecgy1cgy1, that is KTI, lectin, and 7S α' subunit protein free. The new strain has a purple flower, determinate growth habit, and light yellow pods at maturity. The seed has a buffer hilum and is yellow in color. The new strain’s height was 58 cm compared to the Daewonkong cultivar at 46 cm, and its 100-seed weight was 27.1 g, smaller than the Daewonkong at 29.0 g. This is the first new soybean strain with the titilelecgy1cgy1 genotype, and it can be used to improve yellow soybean cultivars of high quality and function.","PeriodicalId":16322,"journal":{"name":"Journal of Life Science","volume":"1 1","pages":"592-597"},"PeriodicalIF":0.0,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90365676","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Application of Response Surface Methodology in Medium Optimization to Improve Lactic Acid Production by Lactobacillus paracasei SRCM201474 响应面法在副干酪乳杆菌SRCM201474培养基优化中的应用
Pub Date : 2020-01-01 DOI: 10.5352/JLS.2020.30.6.522
Gwangsu Ha, Kim Jin Won, Sua Im, Su-Jin Shin, Hee-Jong Yang, Do-Youn Jeong
The aim of this study was to establish the optimal medium composition for enhancing L(+)-lactic acid (LLA) production using response surface methodology (RSM). Lactobacillus paracasei SRCM201474 was selected as the LLA producer by productivity analysis from nine candidates isolated from kimchi and identified by 16S rRNA gene sequencing. Plackett-Burman design was used to assess the effect of eleven media components on LLA production, including carbon (glucose, sucrose, molasses), nitrogen (yeast extract, peptone, tryptone, beef extract), and mineral (NaCl, K2HPO4, MgSO4, MnSO4) materials. Glucose, sucrose, molasses, and peptone were subsequently chosen as promising media for further optimization studies, and a hybrid design experiment was used to establish their optimal concentrations as glucose 15.48 g/l, sucrose 16.73 g/l, molasses 39.09 g/l, and peptone 34.91 g/l. The coefficient of determination of the equation derived from RSM regression for LLA production was mathematically reliable at 0.9969. At optimum parameters, 33.38 g/l of maximum LLA increased by 193% when compared with MRS broth as unoptimized medium (17.66 g/l). Our statistical model was confirmed by subsequent validation experiments. Increasing the performance of LLA-producing microorganisms and establishing an effective LLA fermentation process can be of particular benefit for bioplastic technologies and industrial applications.
本研究的目的是利用响应面法(RSM)确定提高L(+)-乳酸(LLA)产量的最佳培养基组成。从9株泡菜中分离得到副干酪乳杆菌SRCM201474作为LLA产生菌,并通过16S rRNA基因测序进行鉴定。采用Plackett-Burman设计评估了11种培养基成分对LLA生产的影响,包括碳(葡萄糖、蔗糖、糖蜜)、氮(酵母提取物、蛋白胨、色氨酸、牛肉提取物)和矿物(NaCl、K2HPO4、MgSO4、MnSO4)材料。随后选择葡萄糖、蔗糖、糖蜜和蛋白胨作为进一步优化研究的有希望的培养基,并通过混合设计实验确定其最佳浓度为葡萄糖15.48 g/l,蔗糖16.73 g/l,糖蜜39.09 g/l,蛋白胨34.91 g/l。由RSM回归得出的LLA产量方程的决定系数为0.9969,在数学上是可靠的。在优化条件下,最大LLA为33.38 g/l,比未优化培养基MRS肉汤(17.66 g/l)提高了193%。我们的统计模型被后续的验证实验所证实。提高产LLA微生物的性能和建立有效的LLA发酵工艺对生物塑料技术和工业应用具有特别的好处。
{"title":"Application of Response Surface Methodology in Medium Optimization to Improve Lactic Acid Production by Lactobacillus paracasei SRCM201474","authors":"Gwangsu Ha, Kim Jin Won, Sua Im, Su-Jin Shin, Hee-Jong Yang, Do-Youn Jeong","doi":"10.5352/JLS.2020.30.6.522","DOIUrl":"https://doi.org/10.5352/JLS.2020.30.6.522","url":null,"abstract":"The aim of this study was to establish the optimal medium composition for enhancing L(+)-lactic acid (LLA) production using response surface methodology (RSM). Lactobacillus paracasei SRCM201474 was selected as the LLA producer by productivity analysis from nine candidates isolated from kimchi and identified by 16S rRNA gene sequencing. Plackett-Burman design was used to assess the effect of eleven media components on LLA production, including carbon (glucose, sucrose, molasses), nitrogen (yeast extract, peptone, tryptone, beef extract), and mineral (NaCl, K2HPO4, MgSO4, MnSO4) materials. Glucose, sucrose, molasses, and peptone were subsequently chosen as promising media for further optimization studies, and a hybrid design experiment was used to establish their optimal concentrations as glucose 15.48 g/l, sucrose 16.73 g/l, molasses 39.09 g/l, and peptone 34.91 g/l. The coefficient of determination of the equation derived from RSM regression for LLA production was mathematically reliable at 0.9969. At optimum parameters, 33.38 g/l of maximum LLA increased by 193% when compared with MRS broth as unoptimized medium (17.66 g/l). Our statistical model was confirmed by subsequent validation experiments. Increasing the performance of LLA-producing microorganisms and establishing an effective LLA fermentation process can be of particular benefit for bioplastic technologies and industrial applications.","PeriodicalId":16322,"journal":{"name":"Journal of Life Science","volume":"25 1","pages":"522-531"},"PeriodicalIF":0.0,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74540791","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
期刊
Journal of Life Science
全部 Acc. Chem. Res. ACS Applied Bio Materials ACS Appl. Electron. Mater. ACS Appl. Energy Mater. ACS Appl. Mater. Interfaces ACS Appl. Nano Mater. ACS Appl. Polym. Mater. ACS BIOMATER-SCI ENG ACS Catal. ACS Cent. Sci. ACS Chem. Biol. ACS Chemical Health & Safety ACS Chem. Neurosci. ACS Comb. Sci. ACS Earth Space Chem. ACS Energy Lett. ACS Infect. Dis. ACS Macro Lett. ACS Mater. Lett. ACS Med. Chem. Lett. ACS Nano ACS Omega ACS Photonics ACS Sens. ACS Sustainable Chem. Eng. ACS Synth. Biol. Anal. Chem. BIOCHEMISTRY-US Bioconjugate Chem. BIOMACROMOLECULES Chem. Res. Toxicol. Chem. Rev. Chem. Mater. CRYST GROWTH DES ENERG FUEL Environ. Sci. Technol. Environ. Sci. Technol. Lett. Eur. J. Inorg. Chem. IND ENG CHEM RES Inorg. Chem. J. Agric. Food. Chem. J. Chem. Eng. Data J. Chem. Educ. J. Chem. Inf. Model. J. Chem. Theory Comput. J. Med. Chem. J. Nat. Prod. J PROTEOME RES J. Am. Chem. Soc. LANGMUIR MACROMOLECULES Mol. Pharmaceutics Nano Lett. Org. Lett. ORG PROCESS RES DEV ORGANOMETALLICS J. Org. Chem. J. Phys. Chem. J. Phys. Chem. A J. Phys. Chem. B J. Phys. Chem. C J. Phys. Chem. Lett. Analyst Anal. Methods Biomater. Sci. Catal. Sci. Technol. Chem. Commun. Chem. Soc. Rev. CHEM EDUC RES PRACT CRYSTENGCOMM Dalton Trans. Energy Environ. Sci. ENVIRON SCI-NANO ENVIRON SCI-PROC IMP ENVIRON SCI-WAT RES Faraday Discuss. Food Funct. Green Chem. Inorg. Chem. Front. Integr. Biol. J. Anal. At. Spectrom. J. Mater. Chem. A J. Mater. Chem. B J. Mater. Chem. C Lab Chip Mater. Chem. Front. Mater. Horiz. MEDCHEMCOMM Metallomics Mol. Biosyst. Mol. Syst. Des. Eng. Nanoscale Nanoscale Horiz. Nat. Prod. Rep. New J. Chem. Org. Biomol. Chem. Org. Chem. Front. PHOTOCH PHOTOBIO SCI PCCP Polym. Chem.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1