Pub Date : 2024-02-29DOI: 10.55522/jmpas.v13i1.6154
Su Djie to Rante
Hip replacement surgery, a significant orthopaedic intervention, is commonly undertaken to address hip pain stemming from aging or injury, particularly among elderly patients. The primary objective of this surgical procedure is to restore the patient's quality of life to its pre-operative state, enabling them to resume normal daily activities. Typically, the posterior approach has been the conventional surgical method for hip replacement, widely practiced both in Indonesia and globally. This evolving trend has sparked interest in comparing the effectiveness and outcomes of the anterior and posterior approaches, particularly concerning critical factors such as operating time, length of hospital stays, need for transfusion, and postoperative mobilization time. In pursuit of a comprehensive understanding, a study was conducted, focusing on patients who underwent hip replacement surgery at Siloam Kupang Hospital. The anterior approach was considered as the case group, while the posterior approach served as the control group. Surprisingly, the study did not identify any statistically significant differences in operating time and transfusion requirements between hip replacement surgeries utilizing the anterior approach and those employing the posterior approach. This suggests that, from a procedural standpoint, both approaches are comparable in terms of efficiency and blood management. However, when assessing postoperative outcomes, distinct trends emerged. The anterior approach demonstrated a notable advantage in terms of faster mobilization times, implying a quicker recovery and the potential for patients to regain their mobility sooner. On the other hand, the posterior approach exhibited a shorter hospital stay, suggesting a streamlined postoperative course. These findings contribute valuable insights to the ongoing discourse within the orthopaedic community regarding the merits of the anterior and posterior approaches in hip replacement surgery. As medical practices continue to evolve, such comparative studies play a pivotal role in refining surgical techniques and optimizing patient outcomes in the realm of orthopaedic interventions.
{"title":"Comparative Analysis of Anterior and Posterior Approaches in Hip Replacement\u0000 Surgery: Enhancing Patient Efficiency","authors":"Su Djie to Rante","doi":"10.55522/jmpas.v13i1.6154","DOIUrl":"https://doi.org/10.55522/jmpas.v13i1.6154","url":null,"abstract":"Hip replacement surgery, a significant orthopaedic intervention, is commonly\u0000 undertaken to address hip pain stemming from aging or injury, particularly among elderly\u0000 patients. The primary objective of this surgical procedure is to restore the patient's\u0000 quality of life to its pre-operative state, enabling them to resume normal daily\u0000 activities. Typically, the posterior approach has been the conventional surgical method\u0000 for hip replacement, widely practiced both in Indonesia and globally. This evolving\u0000 trend has sparked interest in comparing the effectiveness and outcomes of the anterior\u0000 and posterior approaches, particularly concerning critical factors such as operating\u0000 time, length of hospital stays, need for transfusion, and postoperative mobilization\u0000 time. In pursuit of a comprehensive understanding, a study was conducted, focusing on\u0000 patients who underwent hip replacement surgery at Siloam Kupang Hospital. The anterior\u0000 approach was considered as the case group, while the posterior approach served as the\u0000 control group. Surprisingly, the study did not identify any statistically significant\u0000 differences in operating time and transfusion requirements between hip replacement\u0000 surgeries utilizing the anterior approach and those employing the posterior approach.\u0000 This suggests that, from a procedural standpoint, both approaches are comparable in\u0000 terms of efficiency and blood management. However, when assessing postoperative\u0000 outcomes, distinct trends emerged. The anterior approach demonstrated a notable\u0000 advantage in terms of faster mobilization times, implying a quicker recovery and the\u0000 potential for patients to regain their mobility sooner. On the other hand, the posterior\u0000 approach exhibited a shorter hospital stay, suggesting a streamlined postoperative\u0000 course. These findings contribute valuable insights to the ongoing discourse within the\u0000 orthopaedic community regarding the merits of the anterior and posterior approaches in\u0000 hip replacement surgery. As medical practices continue to evolve, such comparative\u0000 studies play a pivotal role in refining surgical techniques and optimizing patient\u0000 outcomes in the realm of orthopaedic interventions.","PeriodicalId":16445,"journal":{"name":"Journal of Medical pharmaceutical and allied sciences","volume":"14 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140414986","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-02-29DOI: 10.55522/jmpas.v13i1.5882
S. E. Nugraha
The objective of this investigation was to determine the toxicity, anti-anemia activity, betanin content, flavonoid content, and antioxidant capacity of Beta vulgaris L. Microwave-assisted extraction (MAE) was implemented to optimize the extraction procedure. The total phenolic content (TPC) and total flavonoid content (TFC) of the extracts were determined using the Folin-Ciocalteu colorimetric and aluminum chloride methods, respectively. Furthermore, the 2,2-diphenyl-1-picrylhydrazyl (DPPH) method was employed to ascertain antioxidant capacity, whereas thin-layer chromatography was used to identify betanin content. The toxicity and anti-anemia properties were assessed in silico. The in-silico methods encompass several steps: ligand and target protein synthesis, docking of betanin compounds with the erythropoietin receptor (EpoR) target protein via SwissDock, docking data visualization via USCF Chimera, and toxicity analysis via the pkCSM and Protox online tools. The findings indicated that the beetroot extract contained a total of 34.96 ± 3.97 mg GAE/g sample of phenol. The flavonoid content of beetroot was 4.93 ± 0.33 mg QE/g sample. Qualitative analysis conducted using thin-layer chromatography yielded results indicating the presence of betanin in the extract. The IC50 value for the antioxidant activity was 97.31 μg/ml. The docking study revealed the effective interaction of betanin with several amino acid residues, suggesting a low toxicity potential, supported by toxicity class 4 and an LD50 of 305 mg/kg, and the absence of liver and Ames toxicity. It concluded that betanin is a promising anti-anemia agent. levels.
{"title":"Assessing the toxicity and anti-anemia of Beta Vulgaris L.: in silico,\u0000 phytochemical and antioxidant analysis","authors":"S. E. Nugraha","doi":"10.55522/jmpas.v13i1.5882","DOIUrl":"https://doi.org/10.55522/jmpas.v13i1.5882","url":null,"abstract":"The objective of this investigation was to determine the toxicity, anti-anemia\u0000 activity, betanin content, flavonoid content, and antioxidant capacity of Beta vulgaris\u0000 L. Microwave-assisted extraction (MAE) was implemented to optimize the extraction\u0000 procedure. The total phenolic content (TPC) and total flavonoid content (TFC) of the\u0000 extracts were determined using the Folin-Ciocalteu colorimetric and aluminum chloride\u0000 methods, respectively. Furthermore, the 2,2-diphenyl-1-picrylhydrazyl (DPPH) method was\u0000 employed to ascertain antioxidant capacity, whereas thin-layer chromatography was used\u0000 to identify betanin content. The toxicity and anti-anemia properties were assessed in\u0000 silico. The in-silico methods encompass several steps: ligand and target protein\u0000 synthesis, docking of betanin compounds with the erythropoietin receptor (EpoR) target\u0000 protein via SwissDock, docking data visualization via USCF Chimera, and toxicity\u0000 analysis via the pkCSM and Protox online tools. The findings indicated that the beetroot\u0000 extract contained a total of 34.96 ± 3.97 mg GAE/g sample of phenol. The flavonoid\u0000 content of beetroot was 4.93 ± 0.33 mg QE/g sample. Qualitative analysis conducted using\u0000 thin-layer chromatography yielded results indicating the presence of betanin in the\u0000 extract. The IC50 value for the antioxidant activity was 97.31 μg/ml. The docking study\u0000 revealed the effective interaction of betanin with several amino acid residues,\u0000 suggesting a low toxicity potential, supported by toxicity class 4 and an LD50 of 305\u0000 mg/kg, and the absence of liver and Ames toxicity. It concluded that betanin is a\u0000 promising anti-anemia agent. levels.","PeriodicalId":16445,"journal":{"name":"Journal of Medical pharmaceutical and allied sciences","volume":"10 9","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140410448","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-02-29DOI: 10.55522/jmpas.v13i1.4651
Shailendra Saraf
Recently,isoindoline-1,3-dione compounds based folpet, phosmet, captonand thalidomide were developed because they have a comparable degree of anti-proliferative efficacy owing to their diverse mechanisms such as HDAC inhibitors, tryptase inhibitors, inhibits the mode of tnf-α, and angiogenesis inhibitors. It was investigated how the phthalimide pharmacophore interacted with molecules such tnf-α, HDAC, VEGF, EGF, and Tyrosine Kinase Angiogenesis. In order to assess the inhibitory activity against enzyme assay, a series of phthalimidepharmacophores with various substituent’s (Schiff's base) at the N-phenyl ring were submitted to protein-ligand docking investigations using the lib-dock method in the current work. All of the compounds' chemical structures were designed using Cambridge software, ChemBioOffice Ultra 12.0, and their molecular characteristics were determined using the online molecular modelling tool Molinspiration. Utilizing the Discovery Studio Client version 4.1, ADMETlab 2.0, and Lazar 1.4.2 softwares, ADME, Toxicity, and Molecular Docking investigations using the lib-dock method were carried out to evaluate the binding mode and interactions of synthetic hits at the binding site of receptors. Docking studies demonstrated that these sorts of ligands interacted mostly with TNF-α, HDAC, VEGF, EGF, Tyrosine kinase, and angiogenesis reports, among others, by forming hydrogen bonds and interacting hydrophobically with the domain. Our docking results indicate that compounds A1, A10, A11, A22, A26, and A28 demonstrated the greatest binding affinity with the corresponding proteins based on the predicted binding energy. These computer simulations have shown that phthalimide compounds with N-phenyl rings replaced can effectively suppress enzymatic assay.
{"title":"In-silico studies and docking of n-substituted isoindoline-1, 3-dioine analogues as\u0000 anti-proliferative agents","authors":"Shailendra Saraf","doi":"10.55522/jmpas.v13i1.4651","DOIUrl":"https://doi.org/10.55522/jmpas.v13i1.4651","url":null,"abstract":"Recently,isoindoline-1,3-dione compounds based folpet, phosmet, captonand\u0000 thalidomide were developed because they have a comparable degree of anti-proliferative\u0000 efficacy owing to their diverse mechanisms such as HDAC inhibitors, tryptase inhibitors,\u0000 inhibits the mode of tnf-α, and angiogenesis inhibitors. It was investigated how the\u0000 phthalimide pharmacophore interacted with molecules such tnf-α, HDAC, VEGF, EGF, and\u0000 Tyrosine Kinase Angiogenesis. In order to assess the inhibitory activity against enzyme\u0000 assay, a series of phthalimidepharmacophores with various substituent’s (Schiff's base)\u0000 at the N-phenyl ring were submitted to protein-ligand docking investigations using the\u0000 lib-dock method in the current work. All of the compounds' chemical structures were\u0000 designed using Cambridge software, ChemBioOffice Ultra 12.0, and their molecular\u0000 characteristics were determined using the online molecular modelling tool\u0000 Molinspiration. Utilizing the Discovery Studio Client version 4.1, ADMETlab 2.0, and\u0000 Lazar 1.4.2 softwares, ADME, Toxicity, and Molecular Docking investigations using the\u0000 lib-dock method were carried out to evaluate the binding mode and interactions of\u0000 synthetic hits at the binding site of receptors. Docking studies demonstrated that these\u0000 sorts of ligands interacted mostly with TNF-α, HDAC, VEGF, EGF, Tyrosine kinase, and\u0000 angiogenesis reports, among others, by forming hydrogen bonds and interacting\u0000 hydrophobically with the domain. Our docking results indicate that compounds A1, A10,\u0000 A11, A22, A26, and A28 demonstrated the greatest binding affinity with the corresponding\u0000 proteins based on the predicted binding energy. These computer simulations have shown\u0000 that phthalimide compounds with N-phenyl rings replaced can effectively suppress\u0000 enzymatic assay.","PeriodicalId":16445,"journal":{"name":"Journal of Medical pharmaceutical and allied sciences","volume":"9 6","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140412174","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-02-29DOI: 10.55522/jmpas.v13i1.6243
Anamitra Goswami
We are all interested in knowing- whether genes and drugs can increase our life-span. As per Bible, Methuselah's lifespan lasted for a total of 969 years. Recent research has identified the Methuselah gene, a specific DNA segment that holds the potential to promote robust and healthy aging. This discovery opens new avenues for the development of pharmaceutical interventions aimed at extending human lifespan. Aging, a complex process influenced by natural selection, has evolved over time, adapting to factors such as cellular senescence and genetic instability. Research on aging has extensively employed invertebrate models like cnidarians, worms, flies, and yeast. Utilizing genetic methodologies with these organisms has resulted in the identification of numerous aging genes. Remarkably, there is compelling evidence of evolutionary conservation within longevity pathways across diverse species, including mammals. In search of omic study, we would consider data from another set of experiments performed on Cnidarians and show that there has a great advanced on the `biology of aging’ in an indirect way. Cnidarians, like Turritopsis dohrnii, showcase "ontogeny reversal," reverting to earlier stages, thus achieving biological immortality through repeated rejuvenation after reproduction. Alternatively, compounds like resveratrol and rapamycin, have been identified as having the ability to decelerate aging in model organisms. However, as of now, only rapamycin has demonstrated an impact on longevity in experiments on mice. The opportunity to postpone human aging currently exists, whether through established groups of tiny molecules or numerous emerging alternatives. In this context, we explore the approaches to convert findings from age-related research into pharmaceuticals.
{"title":"Decoding the aging nexus: unravelling genetic networks and pharmacological\u0000 strategies for lifespan extension and the methuselah paradox","authors":"Anamitra Goswami","doi":"10.55522/jmpas.v13i1.6243","DOIUrl":"https://doi.org/10.55522/jmpas.v13i1.6243","url":null,"abstract":"We are all interested in knowing- whether genes and drugs can increase our\u0000 life-span. As per Bible, Methuselah's lifespan lasted for a total of 969 years. Recent\u0000 research has identified the Methuselah gene, a specific DNA segment that holds the\u0000 potential to promote robust and healthy aging. This discovery opens new avenues for the\u0000 development of pharmaceutical interventions aimed at extending human lifespan. Aging, a\u0000 complex process influenced by natural selection, has evolved over time, adapting to\u0000 factors such as cellular senescence and genetic instability. Research on aging has\u0000 extensively employed invertebrate models like cnidarians, worms, flies, and yeast.\u0000 Utilizing genetic methodologies with these organisms has resulted in the identification\u0000 of numerous aging genes. Remarkably, there is compelling evidence of evolutionary\u0000 conservation within longevity pathways across diverse species, including mammals. In\u0000 search of omic study, we would consider data from another set of experiments performed\u0000 on Cnidarians and show that there has a great advanced on the `biology of aging’ in an\u0000 indirect way. Cnidarians, like Turritopsis dohrnii, showcase \"ontogeny\u0000 reversal,\" reverting to earlier stages, thus achieving biological immortality\u0000 through repeated rejuvenation after reproduction. Alternatively, compounds like\u0000 resveratrol and rapamycin, have been identified as having the ability to decelerate\u0000 aging in model organisms. However, as of now, only rapamycin has demonstrated an impact\u0000 on longevity in experiments on mice. The opportunity to postpone human aging currently\u0000 exists, whether through established groups of tiny molecules or numerous emerging\u0000 alternatives. In this context, we explore the approaches to convert findings from\u0000 age-related research into pharmaceuticals.","PeriodicalId":16445,"journal":{"name":"Journal of Medical pharmaceutical and allied sciences","volume":"25 10","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140413943","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-10-31DOI: 10.55522/jmpas.v12i5.5640
Aileen May G Ang, Mylene M Uy
Reactive oxygen species (ROS) and cyclooxygenase-2 (COX-2) can be therapeutic targets for inflammatory diseases. COX-2 expression which can be triggered by ROS might consequently promote inflammation. Inhibition of ROS by the antioxidant, however, may prevent or decrease the expression of COX-2. In this study, the antioxidant and anti-inflammatory properties of the hexane (H), dichloromethane (DCM), and methanol-water (MW) extracts of marine sponges Agelas sp. (Asp), Ircinia sp. (Isp), and Aaptos suberitoides (As) were determined. The correlation between their antioxidant properties and ability to inhibit COX enzymes was also established. The MW extracts of the marine sponges and the AsDCM contain a significant (p<0>50% of the COX-2 and gave a selectivity index (COX-2/COX-1) >1.0. This suggests that the extracts can be sources of compounds with promising and selective inhibitory properties against COX-2. Correlation analysis further showed a positive linear correlation between the extracts’ inhibitory activity against the COX enzymes and their TPC, reducing power, and DPPH radical scavenging activity. Thus, the antioxidant activity of the extracts may have influenced their anti-inflammatory property via COX inhibition.
{"title":"Anti-inflammatory and antioxidant properties of Agelas sp., Ircinia sp., and Aaptos suberitoides","authors":"Aileen May G Ang, Mylene M Uy","doi":"10.55522/jmpas.v12i5.5640","DOIUrl":"https://doi.org/10.55522/jmpas.v12i5.5640","url":null,"abstract":"Reactive oxygen species (ROS) and cyclooxygenase-2 (COX-2) can be therapeutic targets for inflammatory diseases. COX-2 expression which can be triggered by ROS might consequently promote inflammation. Inhibition of ROS by the antioxidant, however, may prevent or decrease the expression of COX-2. In this study, the antioxidant and anti-inflammatory properties of the hexane (H), dichloromethane (DCM), and methanol-water (MW) extracts of marine sponges Agelas sp. (Asp), Ircinia sp. (Isp), and Aaptos suberitoides (As) were determined. The correlation between their antioxidant properties and ability to inhibit COX enzymes was also established. The MW extracts of the marine sponges and the AsDCM contain a significant (p<0>50% of the COX-2 and gave a selectivity index (COX-2/COX-1) >1.0. This suggests that the extracts can be sources of compounds with promising and selective inhibitory properties against COX-2. Correlation analysis further showed a positive linear correlation between the extracts’ inhibitory activity against the COX enzymes and their TPC, reducing power, and DPPH radical scavenging activity. Thus, the antioxidant activity of the extracts may have influenced their anti-inflammatory property via COX inhibition.","PeriodicalId":16445,"journal":{"name":"Journal of Medical pharmaceutical and allied sciences","volume":"1038 ","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135869949","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Blood glucose levels in the disease activate the free IGF-1 which promotes lipogenesis of sebaceous glands thus leading to acne formation. The study was aimed to analyze the correlation between insulin resistance and the severity of acne vulgaris. A total of 100 patients clinically diagnosed with acne vulgaris were enrolled in the study. The severity of the acne was evaluated using the GAGS system. The HOMA-IR index was used for the analysis of insulin resistance. The mean age of the patients was 22.13 years with a predominance of females i.e., 76%. GAGS score revealed 89% of patients had mild acne, 9% of patients had moderate acne and 2% of patients had severe acne. As per the HOMA-IR index cutoff value of 2.5, 35% of patients in our study had insulin resistance. A significant positive correlation was observed between the acne vulgaris and insulin resistance. Early treatment of insulin resistance can help evaluate a patient’s metabolic aspects that should be helpful in the diagnosis and treatment of various other skin conditions associated with it apart from acne vulgaris such as acanthosis nigricans, acrochordons, etc., and improving the health and the quality of care for these patients.
{"title":"Analysis of correlation between insulin resistance and severity of acne vulgaris","authors":"Shalabh Singla, Rajwinder Singh, Vinay Shanker, Harpreet Kaur Walia, Parul Goyal","doi":"10.55522/jmpas.v12i5.5165","DOIUrl":"https://doi.org/10.55522/jmpas.v12i5.5165","url":null,"abstract":"Blood glucose levels in the disease activate the free IGF-1 which promotes lipogenesis of sebaceous glands thus leading to acne formation. The study was aimed to analyze the correlation between insulin resistance and the severity of acne vulgaris. A total of 100 patients clinically diagnosed with acne vulgaris were enrolled in the study. The severity of the acne was evaluated using the GAGS system. The HOMA-IR index was used for the analysis of insulin resistance. The mean age of the patients was 22.13 years with a predominance of females i.e., 76%. GAGS score revealed 89% of patients had mild acne, 9% of patients had moderate acne and 2% of patients had severe acne. As per the HOMA-IR index cutoff value of 2.5, 35% of patients in our study had insulin resistance. A significant positive correlation was observed between the acne vulgaris and insulin resistance. Early treatment of insulin resistance can help evaluate a patient’s metabolic aspects that should be helpful in the diagnosis and treatment of various other skin conditions associated with it apart from acne vulgaris such as acanthosis nigricans, acrochordons, etc., and improving the health and the quality of care for these patients.","PeriodicalId":16445,"journal":{"name":"Journal of Medical pharmaceutical and allied sciences","volume":"51 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135863952","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-10-31DOI: 10.55522/jmpas.v12i5.5688
Ravindran Muthukumarasamy, Nur Zulaiqah binti Abd Rahman, Shahnaz Majeed, Enrico Magosso, Sreenivas Patro Sisinthy, Sengamalam Radhakrishnan
Metal nanoparticles typically exhibit sizes ranging from 1 to 100 nanometers and can be produced by several techniques, such as green synthesis and chemical synthesis. This study focuses on the green synthesis of copper oxide nanoparticles (CuO-NPs) using aerial extract of Christia vespertilionis. The observation of CuO-NPs formation was made based on the variations in colour exhibited by the extract mixture following the introduction of copper (II) sulphate (CuSO4). The synthesised nanoparticles underwent characterization utilising various instrumentation techniques, including UV-Vis spectrophotometry, FTIR, TEM, SEM, EDX, and TGA. The antibacterial activity of these nanoparticles was assessed by using disc diffusion method, against selected Gram-positive and Gram-negative bacteria. The investigation also encompassed an examination of the combined effect of the nanoparticles and the antibiotic Ciprofloxacin on antibacterial activity. In this study, it was noted that the maximum absorbance of the CuO-NPs occurred at a wavelength of 253nm. The functional groups of the CuO-NPs were confirmed through FTIR spectrum. TEM analysis revealed the average particle size of the CuO-NPs as 62.16 nm. Additionally, examination of SEM images revealed that the particles exhibited irregular shapes while maintaining a homogeneous distribution. The nanoparticles exhibited enhanced antibacterial efficacy against Gram-positive bacteria in comparison to the Gram-negative bacteria that were examined. Significant synergistic effect was shown when the CuoNPs were used with the antibiotic against the bacteria tested. In conclusion, the study conducted demonstrates favourable outcomes as an antibacterial agent and recommended to conduct additional research to investigate the mechanism of action and toxicity of this agent.
金属纳米颗粒的尺寸通常从1纳米到100纳米不等，可以通过几种技术生产，例如绿色合成和化学合成。本研究主要研究了绿色合成氧化铜纳米颗粒(CuO-NPs)的方法。在引入硫酸铜(CuSO4)后，根据萃取混合物所表现出的颜色变化，观察了CuO-NPs的形成。合成的纳米颗粒进行了各种仪器技术的表征，包括紫外可见分光光度法，FTIR, TEM, SEM, EDX和TGA。采用圆盘扩散法测定纳米颗粒对选定的革兰氏阳性菌和革兰氏阴性菌的抑菌活性。研究还包括纳米颗粒和抗生素环丙沙星对抗菌活性的联合作用。在本研究中，我们注意到CuO-NPs的最大吸光度出现在253nm波长处。通过FTIR光谱确定了CuO-NPs的官能团。TEM分析表明，所制备的CuO-NPs的平均粒径为62.16 nm。此外，对扫描电镜图像的检查显示，颗粒在保持均匀分布的同时呈现不规则形状。与检测的革兰氏阴性细菌相比，纳米颗粒对革兰氏阳性细菌的抗菌效果增强。当CuoNPs与抗生素一起使用时，显示出显著的协同效应。综上所述，本研究显示了作为抗菌药物的良好效果，并建议进一步研究该药物的作用机制和毒性。
{"title":"Green synthesis of copper oxide nanoparticles using christia vespertilionis aerial parts extract: A sustainable approach for antibacterial efficacy assessment","authors":"Ravindran Muthukumarasamy, Nur Zulaiqah binti Abd Rahman, Shahnaz Majeed, Enrico Magosso, Sreenivas Patro Sisinthy, Sengamalam Radhakrishnan","doi":"10.55522/jmpas.v12i5.5688","DOIUrl":"https://doi.org/10.55522/jmpas.v12i5.5688","url":null,"abstract":"Metal nanoparticles typically exhibit sizes ranging from 1 to 100 nanometers and can be produced by several techniques, such as green synthesis and chemical synthesis. This study focuses on the green synthesis of copper oxide nanoparticles (CuO-NPs) using aerial extract of Christia vespertilionis. The observation of CuO-NPs formation was made based on the variations in colour exhibited by the extract mixture following the introduction of copper (II) sulphate (CuSO4). The synthesised nanoparticles underwent characterization utilising various instrumentation techniques, including UV-Vis spectrophotometry, FTIR, TEM, SEM, EDX, and TGA. The antibacterial activity of these nanoparticles was assessed by using disc diffusion method, against selected Gram-positive and Gram-negative bacteria. The investigation also encompassed an examination of the combined effect of the nanoparticles and the antibiotic Ciprofloxacin on antibacterial activity. In this study, it was noted that the maximum absorbance of the CuO-NPs occurred at a wavelength of 253nm. The functional groups of the CuO-NPs were confirmed through FTIR spectrum. TEM analysis revealed the average particle size of the CuO-NPs as 62.16 nm. Additionally, examination of SEM images revealed that the particles exhibited irregular shapes while maintaining a homogeneous distribution. The nanoparticles exhibited enhanced antibacterial efficacy against Gram-positive bacteria in comparison to the Gram-negative bacteria that were examined. Significant synergistic effect was shown when the CuoNPs were used with the antibiotic against the bacteria tested. In conclusion, the study conducted demonstrates favourable outcomes as an antibacterial agent and recommended to conduct additional research to investigate the mechanism of action and toxicity of this agent.","PeriodicalId":16445,"journal":{"name":"Journal of Medical pharmaceutical and allied sciences","volume":" 8","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135813729","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-10-31DOI: 10.55522/jmpas.v12i5.5480.
Suraj Singh, Saravanan K
The objective of the research paper to design and development of herbal tablet formulation (HTF) of leaves of Carica Papaya Linn (LCPL) & Moringao leiferaLinn (LMOL) and fruit part of Carrissa CarandusLam (FCCL).Powdered leaves of Carica Papaya Linn & Moringao leifera Linn and fruit part of Carrissa Carandus Lam was macerated in 80% hydro-ethanolic solution for 72 hours .To identify the active constituents, phyto-chemical screening was performed. The extracts were evaluated for the flavonoid and phenolic content by qualitative and quantitative measurement. The Leaves of LCPL& LMOL and fruit of FCCL was extracted by maceration process. Preliminary phytochemical screening was performed. The LCPL showed the presence of flavonoids, phenol, proteins, glycosides, and alkaloids. The LMOL has shown the presence of phenolic compounds, flavonoids, carbohydrates, proteins, amino acids, terpenoids and tannins. The FCCL has shown the presence of phenolic compounds, flavonoids, carbohydrates, terpenoids, glycosides, alkaloids and tannins. Total flavonoid content and phenolic content was present as gallic acid and Iso-quercetin respectively. The herbal tablet formulation was prepared by wet granulation method utilizing the different proportion of the plant extract. The evaluation of Herbal tablet formulation stated that HTF-3 formulation has shown the best result in pre-compression and post-compression parameters. The hydro ethanolic extract of LCPL, LMOL and FCCL show the presence of different flavonoid and phenolic content i.e., Iso-quercetin, gallic acid. The Three different batches of HTF were prepared and result data indicates that developed formulation has been approved, suitable for oral administration and pharmacological evaluation.
本研究的目的是设计和开发番木瓜叶(Carica Papaya Linn, LCPL)的中药片剂。Carrissa CarandusLam (FCCL)的辣木精(LMOL)和果实部分。番木瓜叶粉;采用80%水乙醇浸泡法，对辣木和山茱萸果实部分进行72 h的植物化学筛选，鉴定其有效成分。采用定性和定量的方法对提取物的类黄酮和酚类含量进行评价。lcpl的叶片采用浸渍法提取枸杞子中LMOL和果实。初步进行了植物化学筛选。结果表明，黄酮类化合物、酚类化合物、蛋白质、糖苷类化合物和生物碱类化合物均存在。LMOL中含有酚类化合物、黄酮类化合物、碳水化合物、蛋白质、氨基酸、萜类化合物和单宁。FCCL中含有酚类化合物、类黄酮、碳水化合物、萜类、苷类、生物碱和单宁。总黄酮含量和酚类含量分别以没食子酸和异槲皮素的形式存在。以不同比例的植物提取物为原料，采用湿造粒法制备中药片剂。中药片剂制剂评价结果表明，HTF-3制剂在预压缩和后压缩参数上效果最佳。LCPL、LMOL和FCCL的水乙醇提取物显示出不同的类黄酮和酚类含量，即异槲皮素、没食子酸。制备了3个不同批次的HTF，结果表明所研制的制剂已获批准，适合口服给药和药理评价。
{"title":"Design and evaluation of herbal tablet formulation (HTF) of leaves of Carica papaya linn, Moringa oleifera linn and fruit of Carrissa carandus lam","authors":"Suraj Singh, Saravanan K","doi":"10.55522/jmpas.v12i5.5480.","DOIUrl":"https://doi.org/10.55522/jmpas.v12i5.5480.","url":null,"abstract":"The objective of the research paper to design and development of herbal tablet formulation (HTF) of leaves of Carica Papaya Linn (LCPL) & Moringao leiferaLinn (LMOL) and fruit part of Carrissa CarandusLam (FCCL).Powdered leaves of Carica Papaya Linn & Moringao leifera Linn and fruit part of Carrissa Carandus Lam was macerated in 80% hydro-ethanolic solution for 72 hours .To identify the active constituents, phyto-chemical screening was performed. The extracts were evaluated for the flavonoid and phenolic content by qualitative and quantitative measurement. The Leaves of LCPL& LMOL and fruit of FCCL was extracted by maceration process. Preliminary phytochemical screening was performed. The LCPL showed the presence of flavonoids, phenol, proteins, glycosides, and alkaloids. The LMOL has shown the presence of phenolic compounds, flavonoids, carbohydrates, proteins, amino acids, terpenoids and tannins. The FCCL has shown the presence of phenolic compounds, flavonoids, carbohydrates, terpenoids, glycosides, alkaloids and tannins. Total flavonoid content and phenolic content was present as gallic acid and Iso-quercetin respectively. The herbal tablet formulation was prepared by wet granulation method utilizing the different proportion of the plant extract. The evaluation of Herbal tablet formulation stated that HTF-3 formulation has shown the best result in pre-compression and post-compression parameters. The hydro ethanolic extract of LCPL, LMOL and FCCL show the presence of different flavonoid and phenolic content i.e., Iso-quercetin, gallic acid. The Three different batches of HTF were prepared and result data indicates that developed formulation has been approved, suitable for oral administration and pharmacological evaluation.","PeriodicalId":16445,"journal":{"name":"Journal of Medical pharmaceutical and allied sciences","volume":"89 3","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135871361","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
This article describes the case of a previously healthy immune-competent child who, despite having received full protection against poliomyelitis with OPV, developed severe flaccid paralysis which eventually led to his death. Poliovirus testing of his contacts revealed the presence of Sabin-like type 3 poliovirus in one of them. The patient developed paralysis and respiratory distress and deceased a few months after the onset of paralysis with respiratory failure. This tragic case report demonstrates the emergence of VAPP and emphasizes the significance of early diagnosis of VAPP infections to enhance the clinical management of VAPP-infected patients.
{"title":"A case of vaccine associated paralytic poliomyelitis in an immune competent child in Morocco","authors":"Sanae Lemrabet, Abdelkarim Filali-Maltouf, Leila Medraoui, Hicham Oumzil","doi":"10.55522/jmpas.v12i5.5482","DOIUrl":"https://doi.org/10.55522/jmpas.v12i5.5482","url":null,"abstract":"This article describes the case of a previously healthy immune-competent child who, despite having received full protection against poliomyelitis with OPV, developed severe flaccid paralysis which eventually led to his death. Poliovirus testing of his contacts revealed the presence of Sabin-like type 3 poliovirus in one of them. The patient developed paralysis and respiratory distress and deceased a few months after the onset of paralysis with respiratory failure. This tragic case report demonstrates the emergence of VAPP and emphasizes the significance of early diagnosis of VAPP infections to enhance the clinical management of VAPP-infected patients.","PeriodicalId":16445,"journal":{"name":"Journal of Medical pharmaceutical and allied sciences","volume":"148 19","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135863284","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-10-31DOI: 10.55522/jmpas.v12i5.4784
Pramod Baburao Patil, Subhash Trimbakrao Kumbhar
Pyrimidinesare six-membered heterocyclic scaffold present naturally in nucleic acid components and are promising leads for the synthesis of medicinally important compounds. Cyclin-dependent kinases (CDKs) with a serine/threonine catalytic core are important druggable targets for cancer therapy and the binding of regulatory subunits controls them. In the present study series of virtually designed pyrimidine derivatives were screened using molecular docking techniques against the cyclin-dependent kinase-8 (CDK8) as a targeted protein. The density functional theory calculation of compounds having good binding affinity was done to estimate the orbital energy. The molecular dynamics simulation of the best-docked compound with the CDK8 was simulated to estimate the effect of mobility on the interactions.Themolecular docking provided insights regarding the binding ability of the designed compounds with the targeted CSK8 structure. As a result, the docked compounds exerted good interactions with the CDK8, and the compound PB129 showed the highest negative binding affinity of -12.4 kcal/mol with the formation of two hydrogen bonds. The results of the simulation study indicated that the complex of CDK-8 and PB129 hasa tight binding with constant hydrogen contacts. Moreover, the density functional theory indicated that PB129 has strong orbital energy and this compound will show tight interactions by either donating or accepting the electron with protein structure. Studied compounds showed good results for the docking study by exerting tight binding with the CDK-8 (PDB 6T41). Compound PB129 showed stable confirmation over the simulation run and has good orbital energies. Compound PB129 may act as a lead against the CDK8.
{"title":"Identification of potential CDK 8 inhibitor from pyrimidine derivatives via In-Silico approach","authors":"Pramod Baburao Patil, Subhash Trimbakrao Kumbhar","doi":"10.55522/jmpas.v12i5.4784","DOIUrl":"https://doi.org/10.55522/jmpas.v12i5.4784","url":null,"abstract":"Pyrimidinesare six-membered heterocyclic scaffold present naturally in nucleic acid components and are promising leads for the synthesis of medicinally important compounds. Cyclin-dependent kinases (CDKs) with a serine/threonine catalytic core are important druggable targets for cancer therapy and the binding of regulatory subunits controls them. In the present study series of virtually designed pyrimidine derivatives were screened using molecular docking techniques against the cyclin-dependent kinase-8 (CDK8) as a targeted protein. The density functional theory calculation of compounds having good binding affinity was done to estimate the orbital energy. The molecular dynamics simulation of the best-docked compound with the CDK8 was simulated to estimate the effect of mobility on the interactions.Themolecular docking provided insights regarding the binding ability of the designed compounds with the targeted CSK8 structure. As a result, the docked compounds exerted good interactions with the CDK8, and the compound PB129 showed the highest negative binding affinity of -12.4 kcal/mol with the formation of two hydrogen bonds. The results of the simulation study indicated that the complex of CDK-8 and PB129 hasa tight binding with constant hydrogen contacts. Moreover, the density functional theory indicated that PB129 has strong orbital energy and this compound will show tight interactions by either donating or accepting the electron with protein structure. Studied compounds showed good results for the docking study by exerting tight binding with the CDK-8 (PDB 6T41). Compound PB129 showed stable confirmation over the simulation run and has good orbital energies. Compound PB129 may act as a lead against the CDK8.","PeriodicalId":16445,"journal":{"name":"Journal of Medical pharmaceutical and allied sciences","volume":"48 8","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135872335","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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