Pub Date : 2023-07-03DOI: 10.55522/jmpas.v12i3.4679
R. Kurniawati, Martini., Nur Endah W, Dwi Sutiningsih
Dengue Hemorrhagic Fever (DHF) is an epidemic disease. In early 2019 the World Health Organization (WHO) determined it as a potential threat. Prevention efforts have been made in biology, chemistry, and behavior. However, the use of chemicals that become instant measures has a negative impact on the occurrence of mosquito resistance and environmental pollution. Natural materials are alternative solutions as natural potentials that are easy, cheap, and safe for human health and environmental health. Cloves are Indonesia's natural potential which contains eugenol, saponins, flavonoids, and tannins which have the potential as active ingredients for Aedes aegypti larvicidal. This study aims to compare the results of the toxicity test of leaf extract with clove flower extract which has the potential as Aedes aegypti larvicidal. The research method uses a laboratory experimental design with a posttest-only control group design. The research sample in the form of third-instar larvae was obtained from the laboratory of the School of Life Sciences and Technology (SITH) Bandung Institute of Technology (ITB) using a purposive sampling method. Comparative data analysis with a numerical variable scale. The results showed that the death effect within 24 hours was between 19% - 92% mortality with clove leaf extract and 64% - 100% the death effect of Aedes aegypti larvae with clove flower extract. Toxicity test with Clove leaf extract obtained LC50 of 7.761% and LC90 of 37.014%. While the clove flower extract obtained LC50 of 3.374% and LC90 of 4.441%.
{"title":"Toxicity test of clove leaf extract compared to clove flower extract (syzygium aromaticum) as aedes aegypti linnaeus larvacide","authors":"R. Kurniawati, Martini., Nur Endah W, Dwi Sutiningsih","doi":"10.55522/jmpas.v12i3.4679","DOIUrl":"https://doi.org/10.55522/jmpas.v12i3.4679","url":null,"abstract":"Dengue Hemorrhagic Fever (DHF) is an epidemic disease. In early 2019 the World Health Organization (WHO) determined it as a potential threat. Prevention efforts have been made in biology, chemistry, and behavior. However, the use of chemicals that become instant measures has a negative impact on the occurrence of mosquito resistance and environmental pollution. Natural materials are alternative solutions as natural potentials that are easy, cheap, and safe for human health and environmental health. Cloves are Indonesia's natural potential which contains eugenol, saponins, flavonoids, and tannins which have the potential as active ingredients for Aedes aegypti larvicidal. This study aims to compare the results of the toxicity test of leaf extract with clove flower extract which has the potential as Aedes aegypti larvicidal. The research method uses a laboratory experimental design with a posttest-only control group design. The research sample in the form of third-instar larvae was obtained from the laboratory of the School of Life Sciences and Technology (SITH) Bandung Institute of Technology (ITB) using a purposive sampling method. Comparative data analysis with a numerical variable scale. The results showed that the death effect within 24 hours was between 19% - 92% mortality with clove leaf extract and 64% - 100% the death effect of Aedes aegypti larvae with clove flower extract. Toxicity test with Clove leaf extract obtained LC50 of 7.761% and LC90 of 37.014%. While the clove flower extract obtained LC50 of 3.374% and LC90 of 4.441%.","PeriodicalId":16445,"journal":{"name":"Journal of Medical pharmaceutical and allied sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88082498","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-07-03DOI: 10.55522/jmpas.v12i3.5018
G. Chandra, H. Haridas, Ellathuveettil Swaminathan, S. Bhattacharya, Ravi Varma Mundakkara Kovilakam, Baiju Govindan Nair, Abey Joseph
Innovating green pathways for fabricating metal nanoparticles (MNPs) turns into an essential subject to promote sustainability and protect our environment from toxicity. Carica papaya is one of the most common cultivations with enriched phytochemicals. Here in this work, we have used male Carica papaya extract (CPE) from its flowers to synthesize eco-friendly silver nanoparticles (AgNPs). The physicochemical properties of CPE-reduced AgNPs (CPE-AgNPs) were studied by different spectroscopic techniques. The characteristic property of AgNPs has been confirmed by analyzing the surface plasmon resonance (SPR) band of CPE- AgNPs: at around 440 nm. The capping of as-prepared AgNPs mediated by CPE was evaluated based on the vibrational bands of functional groups by Fourier Transform Infra-Red (FTIR) spectrometer. The elemental silver composition present in CPE-AgNPs was evidently seen from the corresponding Energy Dispersive X-ray spectroscopic (EDS) measurements. Studies obtained from Transmission Electron Microscopy (TEM) images of CPE-AgNPs reveals that the majority of the CPE-AgNPs are spheres of 11.10 ± 2.30 nm diameter. The Zeta potential value for CPE-AgNPs prepared using 2 ml of CPE was 65.0 ± 2.1 mV, which ensured better stability, and the sample was selected for further studies. CPE-AgNPs show better antibacterial efficacy against bacterial strains and suggest that they can be used for developing AgNPs based medical devices such as urinary catheters to treat urinary tract infections (UTIs).
创新制造金属纳米颗粒(MNPs)的绿色途径已成为促进可持续发展和保护环境免受毒性影响的重要课题。番木瓜是最常见的富含植物化学物质的栽培品种之一。在本研究中，我们利用雄番木瓜花提取物(CPE)合成了环保银纳米粒子(AgNPs)。采用不同的光谱技术研究了CPE-AgNPs (CPE-AgNPs)的物理化学性质。通过分析CPE- AgNPs在440 nm左右的表面等离子体共振(SPR)波段，证实了AgNPs的特性。利用傅里叶变换红外光谱仪(FTIR)对CPE介导的AgNPs的盖度进行了评价。从相应的能量色散x射线光谱(EDS)测量中可以明显地看出CPE-AgNPs中存在元素银的组成。对CPE-AgNPs的透射电镜(TEM)研究表明，CPE-AgNPs大部分为直径为11.10±2.30 nm的球体。使用2 ml CPE制备的CPE- agnps的Zeta电位值为65.0±2.1 mV，稳定性较好，选择样品进行进一步研究。CPE-AgNPs对细菌菌株具有较好的抗菌效果，可用于开发基于AgNPs的医疗器械，如治疗尿路感染的导尿管。
{"title":"Phytochemically enriched male papaya flowers: a better green candidate for silver nanoparticle synthesis and exploring its antibacterial potency","authors":"G. Chandra, H. Haridas, Ellathuveettil Swaminathan, S. Bhattacharya, Ravi Varma Mundakkara Kovilakam, Baiju Govindan Nair, Abey Joseph","doi":"10.55522/jmpas.v12i3.5018","DOIUrl":"https://doi.org/10.55522/jmpas.v12i3.5018","url":null,"abstract":"Innovating green pathways for fabricating metal nanoparticles (MNPs) turns into an essential subject to promote sustainability and protect our environment from toxicity. Carica papaya is one of the most common cultivations with enriched phytochemicals. Here in this work, we have used male Carica papaya extract (CPE) from its flowers to synthesize eco-friendly silver nanoparticles (AgNPs). The physicochemical properties of CPE-reduced AgNPs (CPE-AgNPs) were studied by different spectroscopic techniques. The characteristic property of AgNPs has been confirmed by analyzing the surface plasmon resonance (SPR) band of CPE- AgNPs: at around 440 nm. The capping of as-prepared AgNPs mediated by CPE was evaluated based on the vibrational bands of functional groups by Fourier Transform Infra-Red (FTIR) spectrometer. The elemental silver composition present in CPE-AgNPs was evidently seen from the corresponding Energy Dispersive X-ray spectroscopic (EDS) measurements. Studies obtained from Transmission Electron Microscopy (TEM) images of CPE-AgNPs reveals that the majority of the CPE-AgNPs are spheres of 11.10 ± 2.30 nm diameter. The Zeta potential value for CPE-AgNPs prepared using 2 ml of CPE was 65.0 ± 2.1 mV, which ensured better stability, and the sample was selected for further studies. CPE-AgNPs show better antibacterial efficacy against bacterial strains and suggest that they can be used for developing AgNPs based medical devices such as urinary catheters to treat urinary tract infections (UTIs).","PeriodicalId":16445,"journal":{"name":"Journal of Medical pharmaceutical and allied sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72703259","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-07-03DOI: 10.55522/jmpas.v12i3.5124
D. Manral, J. Sethi, Ankit Jain, Vimal Sharma
The study aimed to find out the effect of core-shoulder stability exercises on shoulder performance among throwers with glenohumeral internal rotation deficit (GIRD). Out of Forty-two athletes who participated, 30 subjects were selected who fulfilled the selection criteria and were randomized into 2 groups with 15 subjects in each group. Throwing accuracy (functional throwing performance index), throwing distance (overall medicine throw test), and shoulder range of motion were assessed and recorded at baseline, 3rd week, and 6th week of intervention. The control group received shoulder stability exercises while the study group received core-shoulder stability exercises 4 days per week for 6 consecutive weeks. Repeated measure ANOVA was used to see the difference between groups (groups 1 and 2) and time (baseline,3rd week, and 6th week ) Averaged across times statistically significant improvement was found in overhead medicine ball throw in the study group (Group 2) as compared to the control group with p<0.05. No significant difference was found in the functional throwing performance index and range of motion when groups were compared across times. However, for all the outcome measures when compared across groups, a significant difference was found between 6th and 3rd-week scores as compared to baseline scores. This indicates improvements were seen in both groups independently. We conclude that core stability and shoulder stability training both improve the range of motion with throwing accuracy and distance. Core stability training improves throwing distance compared to shoulder stability training in subjects with GIRD.
{"title":"Effect of core-shoulder stability exercises on shoulder performance among throwers with glenohumeral internal rotation deficit","authors":"D. Manral, J. Sethi, Ankit Jain, Vimal Sharma","doi":"10.55522/jmpas.v12i3.5124","DOIUrl":"https://doi.org/10.55522/jmpas.v12i3.5124","url":null,"abstract":"The study aimed to find out the effect of core-shoulder stability exercises on shoulder performance among throwers with glenohumeral internal rotation deficit (GIRD). Out of Forty-two athletes who participated, 30 subjects were selected who fulfilled the selection criteria and were randomized into 2 groups with 15 subjects in each group. Throwing accuracy (functional throwing performance index), throwing distance (overall medicine throw test), and shoulder range of motion were assessed and recorded at baseline, 3rd week, and 6th week of intervention. The control group received shoulder stability exercises while the study group received core-shoulder stability exercises 4 days per week for 6 consecutive weeks. Repeated measure ANOVA was used to see the difference between groups (groups 1 and 2) and time (baseline,3rd week, and 6th week ) Averaged across times statistically significant improvement was found in overhead medicine ball throw in the study group (Group 2) as compared to the control group with p<0.05. No significant difference was found in the functional throwing performance index and range of motion when groups were compared across times. However, for all the outcome measures when compared across groups, a significant difference was found between 6th and 3rd-week scores as compared to baseline scores. This indicates improvements were seen in both groups independently. We conclude that core stability and shoulder stability training both improve the range of motion with throwing accuracy and distance. Core stability training improves throwing distance compared to shoulder stability training in subjects with GIRD.","PeriodicalId":16445,"journal":{"name":"Journal of Medical pharmaceutical and allied sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86438846","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-07-03DOI: 10.55522/jmpas.v12i3.4800
Shweta Jain, Shalini Tripathi, P. Tripathi
The study was performed to identify the imperative pharmacognostic details of berberis aristata DC roots. The root of the plant was standardized by morphologically and microscopically. Further other parameters chemomicroscopical, physical, hytochemical screening and florescence analysis were performed to determine the diagnostic features of berberis aristata DC (B. aristata). Macro and microscopic studies were also reported in the present study. Physicochemical parameters including extractive values, ash values, moisture content have been calculated, which showed 0.79 total ash, 0.05 acid insoluble ash, 20 water soluble ash and 0.90 water insoluble ash. The extractive values of berberis aristata DC were found to be 3.6, 1.2, 0.6 and 2.3 in ethanol, Pet. ether, acetone, and in water respectively. Phytochemical analysis closely revealed the presence of alkaloids in this plant. These outcomes will further help to standardize, identification and in execution research on this plant.
{"title":"Pharmacognostic and phytochemical assay of Berberis Aristata DC roots","authors":"Shweta Jain, Shalini Tripathi, P. Tripathi","doi":"10.55522/jmpas.v12i3.4800","DOIUrl":"https://doi.org/10.55522/jmpas.v12i3.4800","url":null,"abstract":"The study was performed to identify the imperative pharmacognostic details of berberis aristata DC roots. The root of the plant was standardized by morphologically and microscopically. Further other parameters chemomicroscopical, physical, hytochemical screening and florescence analysis were performed to determine the diagnostic features of berberis aristata DC (B. aristata). Macro and microscopic studies were also reported in the present study. Physicochemical parameters including extractive values, ash values, moisture content have been calculated, which showed 0.79 total ash, 0.05 acid insoluble ash, 20 water soluble ash and 0.90 water insoluble ash. The extractive values of berberis aristata DC were found to be 3.6, 1.2, 0.6 and 2.3 in ethanol, Pet. ether, acetone, and in water respectively. Phytochemical analysis closely revealed the presence of alkaloids in this plant. These outcomes will further help to standardize, identification and in execution research on this plant.","PeriodicalId":16445,"journal":{"name":"Journal of Medical pharmaceutical and allied sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87965469","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-07-03DOI: 10.55522/jmpas.v12i3.5049
Mylene Mondarte Uy, Rajane Faith Taga Bautista-Ladrillo
Marine sponge Haliclona sp. was examined for its anticancer potential focusing on the antiproliferative and pro-apoptotic properties against HCT116 (colon) cancer cell line. The 16 fractions obtained from Haliclona sp. were subjected to antiproliferative and pro-apoptotic evaluation via MTT (3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay and Caspase-Glo 3/7 assay, respectively, at 30 μg/mL concentration. Fractions 10, 13, 14, and 15 exhibited a significant decrease (p < 0> 50�ll viability) against HCT116. Only fraction 8 showed a significant increase (p < 0.05) in relative light unit (RLU) and fold change (FC) (1809.78 and 2.42), more than two times larger, compared to the negative control (754.89 RLU and 1.00 FC). This result demands further study on fraction 8 to identify and characterize the structure of the compounds responsible for its bioactivity and hence could be a source of drug leads.
{"title":"Antiproliferative and pro-apoptotic properties against colon carcinoma cell line of the marine sponge haliclona sp. From poblacion, kauswagan, lanao del norte, Philippines","authors":"Mylene Mondarte Uy, Rajane Faith Taga Bautista-Ladrillo","doi":"10.55522/jmpas.v12i3.5049","DOIUrl":"https://doi.org/10.55522/jmpas.v12i3.5049","url":null,"abstract":"Marine sponge Haliclona sp. was examined for its anticancer potential focusing on the antiproliferative and pro-apoptotic properties against HCT116 (colon) cancer cell line. The 16 fractions obtained from Haliclona sp. were subjected to antiproliferative and pro-apoptotic evaluation via MTT (3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay and Caspase-Glo 3/7 assay, respectively, at 30 μg/mL concentration. Fractions 10, 13, 14, and 15 exhibited a significant decrease (p < 0> 50�ll viability) against HCT116. Only fraction 8 showed a significant increase (p < 0.05) in relative light unit (RLU) and fold change (FC) (1809.78 and 2.42), more than two times larger, compared to the negative control (754.89 RLU and 1.00 FC). This result demands further study on fraction 8 to identify and characterize the structure of the compounds responsible for its bioactivity and hence could be a source of drug leads.","PeriodicalId":16445,"journal":{"name":"Journal of Medical pharmaceutical and allied sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88780493","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-07-03DOI: 10.55522/jmpas.v12i3.4666
Neelakanth M. Jeedi, Sandeep Bajji, P. Ronad, S. K. Nimbal, Santosh B. Patil
Pain and inflammation are the major devastating health problems commonly treated with NSAIDs. NSAIDs are regularly employed to relieve pain and inflammation. However, the 1,2,4-triazole derivatives have been scientifically evaluated for their use in relieving pain and inflammation. The current research plan is to explore the analgesic and anti-inflammatory properties of 1,2,4-triazole derivatives. 1,2,4-triazoles was tested for their anti-inflammatory properties using carrageenan and formalin-induced paw oedema, acetic acid-induced writhing investigation, while its peripheral and central analgesic actions were assessed using the hot plate method and Carrageenan induced Air Pouch model respectively. The test compounds were administrated at dose of 55 mg/Kg. The standard Diclofenac drug was administrated at dose of 10 mg/Kg for Acetic acid induced writhing investigation, Anti-inflammatory activity and 10 mg/Kg dose of pentazocine was administrated for hot plate method. Rats given with distilled water (10 mL/kg) were designated as the negative controls. Oral administration of all treatments was used. Evaluation of the analgesic and anti-inflammatory investigation showed that the second compound (5-(4-nitrophenyl)-1-phenyl-1H-1,2,4-triazole-3-(2H)–one declines the number of abdominal cramps caused by 1�etic acid and increases the time of response in hot plate method and decreased the edema induced by carrageenan, formalin also decreased volume of exudates in air pouch model. The results of the research indicate that compound 5-(4-nitrophenyl)-1-phenyl-1H-1,2,4-triazole-3-(2H)-one has shown more effective analgesic and Anti-inflammatory properties than the other two compounds.
{"title":"Analgesic and anti-inflammatory investigation of 1,2,4- triazole derivatives in rats","authors":"Neelakanth M. Jeedi, Sandeep Bajji, P. Ronad, S. K. Nimbal, Santosh B. Patil","doi":"10.55522/jmpas.v12i3.4666","DOIUrl":"https://doi.org/10.55522/jmpas.v12i3.4666","url":null,"abstract":"Pain and inflammation are the major devastating health problems commonly treated with NSAIDs. NSAIDs are regularly employed to relieve pain and inflammation. However, the 1,2,4-triazole derivatives have been scientifically evaluated for their use in relieving pain and inflammation. The current research plan is to explore the analgesic and anti-inflammatory properties of 1,2,4-triazole derivatives. 1,2,4-triazoles was tested for their anti-inflammatory properties using carrageenan and formalin-induced paw oedema, acetic acid-induced writhing investigation, while its peripheral and central analgesic actions were assessed using the hot plate method and Carrageenan induced Air Pouch model respectively. The test compounds were administrated at dose of 55 mg/Kg. The standard Diclofenac drug was administrated at dose of 10 mg/Kg for Acetic acid induced writhing investigation, Anti-inflammatory activity and 10 mg/Kg dose of pentazocine was administrated for hot plate method. Rats given with distilled water (10 mL/kg) were designated as the negative controls. Oral administration of all treatments was used. Evaluation of the analgesic and anti-inflammatory investigation showed that the second compound (5-(4-nitrophenyl)-1-phenyl-1H-1,2,4-triazole-3-(2H)–one declines the number of abdominal cramps caused by 1�etic acid and increases the time of response in hot plate method and decreased the edema induced by carrageenan, formalin also decreased volume of exudates in air pouch model. The results of the research indicate that compound 5-(4-nitrophenyl)-1-phenyl-1H-1,2,4-triazole-3-(2H)-one has shown more effective analgesic and Anti-inflammatory properties than the other two compounds.","PeriodicalId":16445,"journal":{"name":"Journal of Medical pharmaceutical and allied sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87647485","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-07-03DOI: 10.55522/jmpas.v12i3.4974
Lina Cárdenas-Pineda, Karla Bellido-Huaranca, Ana Laurente-Montero, Zelmira Flor De la Cruz Ramos, Cesia Rebeca Zarate Cáceres, Mariza Cárdenas, Alicia, Alva Mantari
The objective of the research work was to determine the oxygen saturation (SpO2) in women of childbearing age who live at altitudes greater than 2800 meters above sea level (m.a.s.l) Huancavelica 2021. The study was observational, prospective, cross-sectional, in 151 healthy women of childbearing age, from 18 to 49 years old, the SpO2 was evaluated, through a pulse oximeter, to all women who attended the consultation between July and August of the year 2021, women without cardiopulmonary pathology were considered within the study group and those who accepted take part. Results: the average of the SpO2, of those who live between 2819 - 3499 m.a.s.l. is 93.72% and between 3500 - 4200 m.a.s.l., it is 90.85%; the minimum SpO2 was 85% and 83% respectively, the maximum SpO2 was 99% in both altitude floors. According to age, 92.91% were between 18 and 19 years old, 93.09% between 20 and 34 years old, and 91.74% between 35 and 49 years old. The average SpO2 of the pregnant women was 92.29% and in the non-pregnant women 92.67%, the minimum SpO2 was 88% and 83% respectively, while the maximum value was the same in both groups 99%. Conclusion: the inhabitants show lower saturations at higher altitudes, and it is observed to decrease after 35 years old, no difference is found between pregnant and non-pregnant women.
{"title":"Quantitative descriptive study of oxygen saturation in women living at altitudes greater than 2800 M.A.S.L. Huancavelica Peru","authors":"Lina Cárdenas-Pineda, Karla Bellido-Huaranca, Ana Laurente-Montero, Zelmira Flor De la Cruz Ramos, Cesia Rebeca Zarate Cáceres, Mariza Cárdenas, Alicia, Alva Mantari","doi":"10.55522/jmpas.v12i3.4974","DOIUrl":"https://doi.org/10.55522/jmpas.v12i3.4974","url":null,"abstract":"The objective of the research work was to determine the oxygen saturation (SpO2) in women of childbearing age who live at altitudes greater than 2800 meters above sea level (m.a.s.l) Huancavelica 2021. The study was observational, prospective, cross-sectional, in 151 healthy women of childbearing age, from 18 to 49 years old, the SpO2 was evaluated, through a pulse oximeter, to all women who attended the consultation between July and August of the year 2021, women without cardiopulmonary pathology were considered within the study group and those who accepted take part. Results: the average of the SpO2, of those who live between 2819 - 3499 m.a.s.l. is 93.72% and between 3500 - 4200 m.a.s.l., it is 90.85%; the minimum SpO2 was 85% and 83% respectively, the maximum SpO2 was 99% in both altitude floors. According to age, 92.91% were between 18 and 19 years old, 93.09% between 20 and 34 years old, and 91.74% between 35 and 49 years old. The average SpO2 of the pregnant women was 92.29% and in the non-pregnant women 92.67%, the minimum SpO2 was 88% and 83% respectively, while the maximum value was the same in both groups 99%. Conclusion: the inhabitants show lower saturations at higher altitudes, and it is observed to decrease after 35 years old, no difference is found between pregnant and non-pregnant women.","PeriodicalId":16445,"journal":{"name":"Journal of Medical pharmaceutical and allied sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79040965","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-07-03DOI: 10.55522/jmpas.v12i3.5021
Silvash Prasad Mishra, R. K. Avvari
MicroRNAs (miRNAs) play a key role in disease manifestation, metabolic processes and immune regulation and modulation of the host. MiRNAs can be found in abundance in fecal samples of humans and are present in intracellular, extracellular fluid and extracellular vesicles. Abundance of miRNAs is found to be in ileal lumen as compared to the colon, whereas gut microbiota is more in colon, not in ileum. Still the effect of miRNA is found where the gut microbiota is more, which is by co-localizing with the nucleic acid of the bacteria. Some human miRNAs can regulate the non-coding RNAs in bacterial species like Escherichia coli and Fusobacterium nucleatum by entering into the bacterial cell in the intestine. By repressing the activity of the noncoding RNAs of intestinal bacterial species, human miRNAs bring about the increase or decrease in population of gut microbiota. With the change in the population of the certain microbiota species in the intestine, the host becomes more susceptible to the dysbiosis and becomes vulnerable to intestinal localization of pathogenic bacteria causing diseases like inflammatory bowel disease. With the usage of bioinformatics tool, this study has deepened into interaction of human miRNA and E. coli non-coding RNA, thereby opening up a detailed analysis into interaction of host with its gut microbiome and how the interaction is responsible for diseases in host.
{"title":"A preliminary study on the effects of human microRNAs on gut microbiota","authors":"Silvash Prasad Mishra, R. K. Avvari","doi":"10.55522/jmpas.v12i3.5021","DOIUrl":"https://doi.org/10.55522/jmpas.v12i3.5021","url":null,"abstract":"MicroRNAs (miRNAs) play a key role in disease manifestation, metabolic processes and immune regulation and modulation of the host. MiRNAs can be found in abundance in fecal samples of humans and are present in intracellular, extracellular fluid and extracellular vesicles. Abundance of miRNAs is found to be in ileal lumen as compared to the colon, whereas gut microbiota is more in colon, not in ileum. Still the effect of miRNA is found where the gut microbiota is more, which is by co-localizing with the nucleic acid of the bacteria. Some human miRNAs can regulate the non-coding RNAs in bacterial species like Escherichia coli and Fusobacterium nucleatum by entering into the bacterial cell in the intestine. By repressing the activity of the noncoding RNAs of intestinal bacterial species, human miRNAs bring about the increase or decrease in population of gut microbiota. With the change in the population of the certain microbiota species in the intestine, the host becomes more susceptible to the dysbiosis and becomes vulnerable to intestinal localization of pathogenic bacteria causing diseases like inflammatory bowel disease. With the usage of bioinformatics tool, this study has deepened into interaction of human miRNA and E. coli non-coding RNA, thereby opening up a detailed analysis into interaction of host with its gut microbiome and how the interaction is responsible for diseases in host.","PeriodicalId":16445,"journal":{"name":"Journal of Medical pharmaceutical and allied sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74677953","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-07-03DOI: 10.55522/jmpas.v12i3.4398
Amit Gupta, Simran Srivastava, Arshya Singh, P. Arya, A. B. Bajpai, Vijay Kumar
Vegetables are parts of plants having several chemical families which belong to the category of antioxidants. In literature, these vegetables and fruits contain high amounts of antioxidant molecules which may directly benefit to human healthcare. The objective of our study is to determine the antioxidant potential of seed-containing fibers aqueous extract and analyzed its phenolic and flavonoid content. For these studies, we procured Phaseolus vulgaris (green beans) from the local market of Dehradun and measured its antioxidant activity including phenolic and flavonoid content. The results of these studies showed that seed-containing fibers aqueous extract showed higher antioxidant activity at higher doses and also showed enhancement in phenolic and flavonoid content. These studies may indicate that vegetables especially Phaseolus vulgaris contain a significant number of antioxidants which is required and acceptable for human healthcare.
{"title":"Total phenolic, flavonoid content and anti-oxidant potential of Phaseolus vulgaris","authors":"Amit Gupta, Simran Srivastava, Arshya Singh, P. Arya, A. B. Bajpai, Vijay Kumar","doi":"10.55522/jmpas.v12i3.4398","DOIUrl":"https://doi.org/10.55522/jmpas.v12i3.4398","url":null,"abstract":"Vegetables are parts of plants having several chemical families which belong to the category of antioxidants. In literature, these vegetables and fruits contain high amounts of antioxidant molecules which may directly benefit to human healthcare. The objective of our study is to determine the antioxidant potential of seed-containing fibers aqueous extract and analyzed its phenolic and flavonoid content. For these studies, we procured Phaseolus vulgaris (green beans) from the local market of Dehradun and measured its antioxidant activity including phenolic and flavonoid content. The results of these studies showed that seed-containing fibers aqueous extract showed higher antioxidant activity at higher doses and also showed enhancement in phenolic and flavonoid content. These studies may indicate that vegetables especially Phaseolus vulgaris contain a significant number of antioxidants which is required and acceptable for human healthcare.","PeriodicalId":16445,"journal":{"name":"Journal of Medical pharmaceutical and allied sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74444775","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-07-03DOI: 10.55522/jmpas.v12i3.5019
V. Prakash, Lipika Parida
Vitamin E is an important food ingredient that individuals ingest to help prevent numerous diseases. Nano-emulsions are frequently employed in pharmaceutical, food, and personal care applications as means of delivering a variety of lipophilic active substances, namely vitamins that are oil-soluble. Both high-energy and low-energy methods are used to create nano-emulsions. The latter, however, offers advantages including less cost, convenience of use, and increased energy efficiency. In this work, we used the emulsion phase inversion technique to create nano-emulsions containing vitamin E. We investigated the rheological and physical characteristics of nano-emulsions created at different stirring rates ranging from 30 to 110 minutes. The emulsion phase inversion approach mixes an organic phase made up of oil, vitamin E, and a surfactant with an aqueous phase. The droplet size, zeta potential, and rheology of all the nano-emulsions were measured. The size distribution of nano-emulsions was measured in the particle size examination method utilizing dynamic light scattering and average droplet diameter was observed to be within a range of 141 nm to 177 nm and to follow a sequence: 110 < 70 < 30 min. The lowest droplet size, 141 nm, with a polydispersity index of 0.234, was obtained at 110 minutes. The zeta potential of formulated nano-emulsions ranged from – 7.1 m to – 14.3 mV. The rheological properties of nano-emulsions revealed non-Newtonian flow behavior. The antimicrobial test of nano-emulsions was examined with Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus), and the emulsions were resistant to S. aureus.
{"title":"Vitamin E encapsulated nano-emulsions formulation, rheological and antimicrobial analysis","authors":"V. Prakash, Lipika Parida","doi":"10.55522/jmpas.v12i3.5019","DOIUrl":"https://doi.org/10.55522/jmpas.v12i3.5019","url":null,"abstract":"Vitamin E is an important food ingredient that individuals ingest to help prevent numerous diseases. Nano-emulsions are frequently employed in pharmaceutical, food, and personal care applications as means of delivering a variety of lipophilic active substances, namely vitamins that are oil-soluble. Both high-energy and low-energy methods are used to create nano-emulsions. The latter, however, offers advantages including less cost, convenience of use, and increased energy efficiency. In this work, we used the emulsion phase inversion technique to create nano-emulsions containing vitamin E. We investigated the rheological and physical characteristics of nano-emulsions created at different stirring rates ranging from 30 to 110 minutes. The emulsion phase inversion approach mixes an organic phase made up of oil, vitamin E, and a surfactant with an aqueous phase. The droplet size, zeta potential, and rheology of all the nano-emulsions were measured. The size distribution of nano-emulsions was measured in the particle size examination method utilizing dynamic light scattering and average droplet diameter was observed to be within a range of 141 nm to 177 nm and to follow a sequence: 110 < 70 < 30 min. The lowest droplet size, 141 nm, with a polydispersity index of 0.234, was obtained at 110 minutes. The zeta potential of formulated nano-emulsions ranged from – 7.1 m to – 14.3 mV. The rheological properties of nano-emulsions revealed non-Newtonian flow behavior. The antimicrobial test of nano-emulsions was examined with Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus), and the emulsions were resistant to S. aureus.","PeriodicalId":16445,"journal":{"name":"Journal of Medical pharmaceutical and allied sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84879812","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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