Pub Date : 2019-01-01DOI: 10.4172/1948-5948.1000417
S. K. Repalli, Chaitanya Kumar Geda, Rao Gjn
As an alternative to the conventional but expensive gold micro carriers regularly employed in micro-projectile bombardment method, this study had utilized, novel, promising but cheaper clay particles-MMT (montmorillonite) as micro-carriers for gene transfer. Gene expression with novel micro carriers was reported through the universally employed GUS expression system at both transient and stable expression levels in comparison to the gold and tungsten. Results suggest that GUS expression levels are higher with MMT than tungsten carriers but lower to gold carriers. It is interesting to note that the GUS expression zones are larger (>1 mm) with MMT carriers in comparison to tungsten (0.6 mm) and gold (0.4 mm). Molecular assays on the transformed cells suggest proper gene delivery by MMT carriers. The results suggest that novel micro carriers can be a viable alternative to gold for gene transfer with high promise in minimizing the costs without compromising the transformation efficiency.
{"title":"MMT, a High Promising, Cost Effective Micro-carrier for Gene Delivery","authors":"S. K. Repalli, Chaitanya Kumar Geda, Rao Gjn","doi":"10.4172/1948-5948.1000417","DOIUrl":"https://doi.org/10.4172/1948-5948.1000417","url":null,"abstract":"As an alternative to the conventional but expensive gold micro carriers regularly employed in micro-projectile bombardment method, this study had utilized, novel, promising but cheaper clay particles-MMT (montmorillonite) as micro-carriers for gene transfer. Gene expression with novel micro carriers was reported through the universally employed GUS expression system at both transient and stable expression levels in comparison to the gold and tungsten. Results suggest that GUS expression levels are higher with MMT than tungsten carriers but lower to gold carriers. It is interesting to note that the GUS expression zones are larger (>1 mm) with MMT carriers in comparison to tungsten (0.6 mm) and gold (0.4 mm). Molecular assays on the transformed cells suggest proper gene delivery by MMT carriers. The results suggest that novel micro carriers can be a viable alternative to gold for gene transfer with high promise in minimizing the costs without compromising the transformation efficiency.","PeriodicalId":16453,"journal":{"name":"Journal of Microbial & Biochemical Technology","volume":"11 1","pages":"62-69"},"PeriodicalIF":0.0,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88878480","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
In order to demonstrate the transmission of resistance genes between clinical and community Pseudomonas, 61 strains of Pseudomonas including 47 (77.04%) community Pseudomonas and 14 (22.96%) clinical Pseudomonas were tested with antibiotics by the method of diffusion of the antibiotic discs in solid medium on Mueller Hinton medium. Thirty four strains of DNA comprising 20 (58.82%) community strains and 14 (41.18%) clinical strains exhibiting colistin resistance were extracted; then, the mcr-1 resistance gene was detected by PCR. The antibiotic sensitivity test showed that tobramycin and ciprofloxacin were more active in community and clinical Pseudomonas. The difference was significant with one p<0.05 for five antibiotics between community and clinical Pseudomonas. PCRs of the mcr-1 gene are positive for 8 (40%) community Pseudomonas strains and 5 (35.71%) clinical Pseudomonas strains. The amplified mcr-1 gene fragments were different in size in clinical Pseudomonas.
{"title":"Study of Colistin Resistance Encoded by the mcr-1 Gene in Community and Clinical Pseudomonas in Brazzaville, Republic of Congo","authors":"Ahombo Gabriel, Baloki Ngoulou Tarcisse, M. Rachel, Kayath Aimé Christian, Ontsira Ngoyi Nina Esther","doi":"10.4172/1948-5948.1000422","DOIUrl":"https://doi.org/10.4172/1948-5948.1000422","url":null,"abstract":"In order to demonstrate the transmission of resistance genes between clinical and community Pseudomonas, 61 strains of Pseudomonas including 47 (77.04%) community Pseudomonas and 14 (22.96%) clinical Pseudomonas were tested with antibiotics by the method of diffusion of the antibiotic discs in solid medium on Mueller Hinton medium. Thirty four strains of DNA comprising 20 (58.82%) community strains and 14 (41.18%) clinical strains exhibiting colistin resistance were extracted; then, the mcr-1 resistance gene was detected by PCR. The antibiotic sensitivity test showed that tobramycin and ciprofloxacin were more active in community and clinical Pseudomonas. The difference was significant with one p<0.05 for five antibiotics between community and clinical Pseudomonas. PCRs of the mcr-1 gene are positive for 8 (40%) community Pseudomonas strains and 5 (35.71%) clinical Pseudomonas strains. The amplified mcr-1 gene fragments were different in size in clinical Pseudomonas.","PeriodicalId":16453,"journal":{"name":"Journal of Microbial & Biochemical Technology","volume":"1 1","pages":"90-96"},"PeriodicalIF":0.0,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91144784","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-01-01DOI: 10.4172/1948-5948.1000419
Piyush Sethia, Manmeet Ahuja, V. Rangaswamy
Isoprene is an industrially important five carbon compound primarily used for production of high quality synthetic rubber. Two major pathways are involved in isoprene synthesis. The mevalonate pathway is present in eukaryotes, archaebacteria and cytosol of higher plants whereas the non-mevalonate pathway exists in many eubacteria and plastids in algae/plants. There have been continuous efforts to study and understand the phenomenon of biological production of isoprene for more than half a century. Although, the current feasibility and cost advantage of chemical processes leading to production of isoprene seems to be far from being dominated by a suitable biological substitute, the fear of extinction of non-renewable resources (raw material for chemical processes) in the near future prompts for a colossal expectation from the synthetic biology community. Technological advances in the field of metabolic engineering have made it possible to vigorously modify and swap genes among different organisms and push the limits for microorganisms to over-produce isoprene to an enormous extent. This review touches upon the limitations faced while improving isoprene titres and the meticulous strategies used to overcome them. It analyzes recent approaches that have resulted in significant improvement of biologically produced isoprene, summarizes the lessons learned from them, and compiles an exhaustive list of potential gene targets that could facilitate prospective research in this widespread arena.
{"title":"Metabolic Engineering of Microorganisms to Produce Isoprene","authors":"Piyush Sethia, Manmeet Ahuja, V. Rangaswamy","doi":"10.4172/1948-5948.1000419","DOIUrl":"https://doi.org/10.4172/1948-5948.1000419","url":null,"abstract":"Isoprene is an industrially important five carbon compound primarily used for production of high quality synthetic rubber. Two major pathways are involved in isoprene synthesis. The mevalonate pathway is present in eukaryotes, archaebacteria and cytosol of higher plants whereas the non-mevalonate pathway exists in many eubacteria and plastids in algae/plants. There have been continuous efforts to study and understand the phenomenon of biological production of isoprene for more than half a century. Although, the current feasibility and cost advantage of chemical processes leading to production of isoprene seems to be far from being dominated by a suitable biological substitute, the fear of extinction of non-renewable resources (raw material for chemical processes) in the near future prompts for a colossal expectation from the synthetic biology community. Technological advances in the field of metabolic engineering have made it possible to vigorously modify and swap genes among different organisms and push the limits for microorganisms to over-produce isoprene to an enormous extent. This review touches upon the limitations faced while improving isoprene titres and the meticulous strategies used to overcome them. It analyzes recent approaches that have resulted in significant improvement of biologically produced isoprene, summarizes the lessons learned from them, and compiles an exhaustive list of potential gene targets that could facilitate prospective research in this widespread arena.","PeriodicalId":16453,"journal":{"name":"Journal of Microbial & Biochemical Technology","volume":"36 1","pages":"73-81"},"PeriodicalIF":0.0,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90249737","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-01-01DOI: 10.4172/1948-5948.1000416
Mansour Bin, Farokh Karimi, S. Rostamnia
In this research, we have tried to maximize the potential of Fe3O4 magnetic nanoparticles coated by SiO2 and immobilized with imidazoline hetropolymer (MNPS-IHSP) to stabilize and release the drug molecule called ampicillin (amp). In this purpose, nanoparticles of 50 nm in size were investigated under a tool such as; FT-IR, SEM, EDX detection and its structure in-vitro conditions. MNPS-IHSP nanoparticles is a specific and suitable nanocomposite that it have a mission as well as stabilizing the amp in the presence of silica coating (electrostatic bonding), that it called MNPS-IHSPA, and the results indicate a stabilization with a higher percentage of 85. It was beneficial to the silica coating. This measurement was performed by a UV-V is spectrophotometer analysis. Ultimately, in-vitro conditions, these magnetic nanoparticles that were migrating with ampicillin was tested on growing bacteria and resulted in the bacterial killing, indicating the antibacterial properties of magnetic nanoparticles. Ultimately, the project tells us that it can also be useful for destroying pathogenic bacteria living cells in vivo conditions.
在这项研究中，我们试图最大限度地发挥Fe3O4磁性纳米颗粒的潜力，这些纳米颗粒被SiO2包裹，并被咪唑啉异聚物(MNPS-IHSP)固定，以稳定和释放药物分子氨苄西林(amp)。为此，在以下工具下研究了50纳米尺寸的纳米颗粒:FT-IR, SEM, EDX检测及其体外结构条件。MNPS-IHSP纳米粒子是一种特殊的、合适的纳米复合材料，它具有在二氧化硅涂层(静电键合)存在的情况下稳定放大器的任务，它被称为MNPS-IHSPA，结果表明稳定率高达85%。它有利于二氧化硅涂层的形成。该测量由紫外-紫外分光光度计分析完成。最后，在体外条件下，这些随氨苄西林迁移的磁性纳米颗粒在生长的细菌上进行了测试，并导致细菌死亡，这表明磁性纳米颗粒的抗菌特性。最终，该项目告诉我们，它也可以用于在体内条件下摧毁致病菌活细胞。
{"title":"Use the Best of MNPS-IHSP Nanoparticles with Coating of Ampicillin Antibiotic, as Bactericidal Properties","authors":"Mansour Bin, Farokh Karimi, S. Rostamnia","doi":"10.4172/1948-5948.1000416","DOIUrl":"https://doi.org/10.4172/1948-5948.1000416","url":null,"abstract":"In this research, we have tried to maximize the potential of Fe3O4 magnetic nanoparticles coated by SiO2 and immobilized with imidazoline hetropolymer (MNPS-IHSP) to stabilize and release the drug molecule called ampicillin (amp). In this purpose, nanoparticles of 50 nm in size were investigated under a tool such as; FT-IR, SEM, EDX detection and its structure in-vitro conditions. MNPS-IHSP nanoparticles is a specific and suitable nanocomposite that it have a mission as well as stabilizing the amp in the presence of silica coating (electrostatic bonding), that it called MNPS-IHSPA, and the results indicate a stabilization with a higher percentage of 85. It was beneficial to the silica coating. This measurement was performed by a UV-V is spectrophotometer analysis. Ultimately, in-vitro conditions, these magnetic nanoparticles that were migrating with ampicillin was tested on growing bacteria and resulted in the bacterial killing, indicating the antibacterial properties of magnetic nanoparticles. Ultimately, the project tells us that it can also be useful for destroying pathogenic bacteria living cells in vivo conditions.","PeriodicalId":16453,"journal":{"name":"Journal of Microbial & Biochemical Technology","volume":"1 1","pages":"55-61"},"PeriodicalIF":0.0,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84443306","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-01-01DOI: 10.35248/1948-5948.19.11.425
Desi Utami, D. Widianto, M. S. Rohman, Heri Hendro Satriyo, Sheila, Julia Anggun, I. D. Prijambada
Petroleum is a complex mixture of hydrocarbons. No single species of microorganisms is able to degrade all components of the petroleum. Mutually beneficial interaction of microorganisms in the form of a consortium is required during the process of oil degradation. One form of mutually beneficial interaction between fungi and bacteria is biofilm formed by bacteria on fungal surface. The formation of bacterial biofilm on fungal surface was reported to increase the synergistic action of the two microorganisms in destroying certain complex compounds. This work was aimed to assess the capacity of hydrocarbon degrading-bacterial biofilm on the surface of hydrocarbon degrading-fungal surface to degrade hydrocarbons derived from drill cuttings. The hydrocarbon degrading-soil bacteria and fungi were isolated from different area in Yogyakarta, Indonesia after enrichment. The ability of bacteria to form biofilm on the surface of fungal hyphae was examined under light microscope with 1000x magnification after the addition of lactophenol. The effect of microbial amendment in the form of biofilm, in comparison with the planktonic culture, on degradation of hydrocarbons derived from drill cuttings were assessed by measuring the extractable petroleum hydrocarbon. The results showed that co-culture between fungi and bacteria which one among them or both of them have low ability to degrade hydrocarbon may significantly improve their ability and the ability of the co-culture to degrade hydrocarbon has no relationship with the ability of the bacteria to form biofilm on the surface of the fungal hyphae.
{"title":"Synergistic Capability of Bacterial-fungal Co-culture to Degrade Drill Cutting Hydrocarbon","authors":"Desi Utami, D. Widianto, M. S. Rohman, Heri Hendro Satriyo, Sheila, Julia Anggun, I. D. Prijambada","doi":"10.35248/1948-5948.19.11.425","DOIUrl":"https://doi.org/10.35248/1948-5948.19.11.425","url":null,"abstract":"Petroleum is a complex mixture of hydrocarbons. No single species of microorganisms is able to degrade all components of the petroleum. Mutually beneficial interaction of microorganisms in the form of a consortium is required during the process of oil degradation. One form of mutually beneficial interaction between fungi and bacteria is biofilm formed by bacteria on fungal surface. The formation of bacterial biofilm on fungal surface was reported to increase the synergistic action of the two microorganisms in destroying certain complex compounds. This work was aimed to assess the capacity of hydrocarbon degrading-bacterial biofilm on the surface of hydrocarbon degrading-fungal surface to degrade hydrocarbons derived from drill cuttings. The hydrocarbon degrading-soil bacteria and fungi were isolated from different area in Yogyakarta, Indonesia after enrichment. The ability of bacteria to form biofilm on the surface of fungal hyphae was examined under light microscope with 1000x magnification after the addition of lactophenol. The effect of microbial amendment in the form of biofilm, in comparison with the planktonic culture, on degradation of hydrocarbons derived from drill cuttings were assessed by measuring the extractable petroleum hydrocarbon. The results showed that co-culture between fungi and bacteria which one among them or both of them have low ability to degrade hydrocarbon may significantly improve their ability and the ability of the co-culture to degrade hydrocarbon has no relationship with the ability of the bacteria to form biofilm on the surface of the fungal hyphae.","PeriodicalId":16453,"journal":{"name":"Journal of Microbial & Biochemical Technology","volume":"25 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89683019","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-01-01DOI: 10.4172/1948-5948.1000412
Koffi Affoué Carole, K. Clément, Kossonou Yao Kamelé, Koffi Ahua René, Koffi-Nevry Rose
This study aims to test different extracts (water, 70% ethanolic-water and acetal) of Capsicum sp. on the growth of Alternaria sp., Penicillium sp., Fusarium sp. and Aspergillus flavus. The nature of the antifungal activity, the antifungal parameters (MIC, MFC, IC50) and the percentage of survival of the strains were determined with the dilution method. On Alternaria sp., Penicillium sp. and Fusarium sp. strains, values of MFC/MIC ratios of water and 70% ethanol extracts of Capsicum annuum antillais were equal to two. Concerning Capsicum frutescens (soudanais, attie, doux and oiseau) varieties, these values varied from 1 to 2 on the fungal strains. These ratios for most of the strains were lower than 4. These extracts exerted a fungistatic action on chosen strains. The curves obtained had a declining appearance with more or less steep slopes depending on the extracts. These curved were used to obtain the inhibitory concentrations for 50% survival (IC50) of the moulds. The highest activity was achieved at the lowest IC50 values which varied from 0.2 mg/mL to 0.3 mg/mL. Therefore, Capsicum extracts proved to be active on in vitro growth of the fungal strains studied in a dose-dependent manner.
{"title":"Effect of Capsicum annuum L. and Capsicum frutescens L. Varieties Extracts on in vitro Growth of Fungal Strains","authors":"Koffi Affoué Carole, K. Clément, Kossonou Yao Kamelé, Koffi Ahua René, Koffi-Nevry Rose","doi":"10.4172/1948-5948.1000412","DOIUrl":"https://doi.org/10.4172/1948-5948.1000412","url":null,"abstract":"This study aims to test different extracts (water, 70% ethanolic-water and acetal) of Capsicum sp. on the growth of Alternaria sp., Penicillium sp., Fusarium sp. and Aspergillus flavus. The nature of the antifungal activity, the antifungal parameters (MIC, MFC, IC50) and the percentage of survival of the strains were determined with the dilution method. On Alternaria sp., Penicillium sp. and Fusarium sp. strains, values of MFC/MIC ratios of water and 70% ethanol extracts of Capsicum annuum antillais were equal to two. Concerning Capsicum frutescens (soudanais, attie, doux and oiseau) varieties, these values varied from 1 to 2 on the fungal strains. These ratios for most of the strains were lower than 4. These extracts exerted a fungistatic action on chosen strains. The curves obtained had a declining appearance with more or less steep slopes depending on the extracts. These curved were used to obtain the inhibitory concentrations for 50% survival (IC50) of the moulds. The highest activity was achieved at the lowest IC50 values which varied from 0.2 mg/mL to 0.3 mg/mL. Therefore, Capsicum extracts proved to be active on in vitro growth of the fungal strains studied in a dose-dependent manner.","PeriodicalId":16453,"journal":{"name":"Journal of Microbial & Biochemical Technology","volume":"12 1","pages":"36-42"},"PeriodicalIF":0.0,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85230812","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-01-01DOI: 10.4172/1948-5948.1000409
E. Ma, Fahmi Ai, R. A. Eissa, El-Zanaty Am
Trichoderma has been used as a biocontrol agent against soil borne diseases that cause economic losses for crops. The objectives of the present investigation were (i) to isolate and characterize Trichoderma spp. from Menoufia Governorate and (ii) to evaluate the isolated Trichoderma spp. as potential biocontrol agents against some soil borne diseases. Soil samples were collected from nine districts and 25 isolates were obtained. Methods of identification of macroscopic and microscopic features, and the sequences of ITS and TEF1-α yielded three species; T. harzianum, T. longibrachiatum and T. asperellum. Phylogenetic tree of the identified 22 strains confirmed that the two strains T. longibrachiatum and T. asperellum came together in the same branch while the rest of the strains which were T. harzianum were on the other side of the tree. All 25 Trichoderma strains and isolates exhibited inhibition to the mycelial growth of four pathogenens. They were antagonized by competition mechanism against 000v m Sclerotium spp., by antibiosis against Fusarium oxysporum and partially against Sclerotium spp. and by mycoparasitism against Rhizoctonia solani. Also, they elucidated differences in total chitinolytic activity measured by two different methods and exochitonolytic activity. Finally, no correlation was found between total chitinolytic activities and total protein contents.
{"title":"Genetic Characterization of Trichoderma spp. Isolated from Different Locations of Menoufia, Egypt and Assessment of their AntagonisticAbility","authors":"E. Ma, Fahmi Ai, R. A. Eissa, El-Zanaty Am","doi":"10.4172/1948-5948.1000409","DOIUrl":"https://doi.org/10.4172/1948-5948.1000409","url":null,"abstract":"Trichoderma has been used as a biocontrol agent against soil borne diseases that cause economic losses for crops. The objectives of the present investigation were (i) to isolate and characterize Trichoderma spp. from Menoufia Governorate and (ii) to evaluate the isolated Trichoderma spp. as potential biocontrol agents against some soil borne diseases. Soil samples were collected from nine districts and 25 isolates were obtained. Methods of identification of macroscopic and microscopic features, and the sequences of ITS and TEF1-α yielded three species; T. harzianum, T. longibrachiatum and T. asperellum. Phylogenetic tree of the identified 22 strains confirmed that the two strains T. longibrachiatum and T. asperellum came together in the same branch while the rest of the strains which were T. harzianum were on the other side of the tree. All 25 Trichoderma strains and isolates exhibited inhibition to the mycelial growth of four pathogenens. They were antagonized by competition mechanism against 000v m Sclerotium spp., by antibiosis against Fusarium oxysporum and partially against Sclerotium spp. and by mycoparasitism against Rhizoctonia solani. Also, they elucidated differences in total chitinolytic activity measured by two different methods and exochitonolytic activity. Finally, no correlation was found between total chitinolytic activities and total protein contents.","PeriodicalId":16453,"journal":{"name":"Journal of Microbial & Biochemical Technology","volume":"56 1","pages":"9-23"},"PeriodicalIF":0.0,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91312233","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-12-01DOI: 10.4172/1948-5948.1000397
Z. Faten, H. Mustafa, M. Ald
Objectives: The present study is designed to evaluate the antitumor activity of sulphur containing compounds in Nano formulations against Ehrlisch Ascites Carcinoma (EAC) in female albino mice. Methods: Preparation of sulfur nanoparticles (S-NPs) was done throughout. Firstly, the evaluation of their composition and purity by fourier transform infrared spectrometer (FT-IR) analysis. Secondly, the shape and size of S-NPs were investigated by scanning electron microscopy (SEM) techniques in all S-NPs samples which were prepared with Tetramethylammoniumbromide (TMAB) surfactant, Then, Energy-dispersive X-ray spectroscopy (EDS) for the evaluation of their composition and purity, after that the X-Ray diffraction patterns were measured for prepared S-NPs with TMAB surfactant, and the determination of S-NPs antitumor activity against (breast carcinoma cell line (MCF7), liver carcinoma cell line (HEPG2), colon carcinoma cell line (HCT116), prostatic carcinoma cell line (PC3). Finally, determination biochemical assay of S-NPs by using female mice bearing EAC and of the assessment of liver functions and evaluation of kidney functions and determination of some heart functions in serum and hematology markers. Results: This study revealed that S-NPs showed high strong activities in concentration (5 mg/kg) as it improvement of liver functions production compared to the positive control group, also showed the effect of S-NPs of the kidney functions, heart functions and hematology parameters in all studied groups and a significant increase in activities compared with positive control group. Conclusion: S-NPs play an important role in improving liver functions, kidney functions and the heart functions of resulted in significantly increase activity against tumour and plays an important role in improving oxidative stress resulted in high antiproliferation activity against (MCF-7, HepG2, HCT116 and PC3) cell lines, and significantly increase with S-NPs reduced most of the hematological parameters towards the best compared to the positive control group.
{"title":"Synthesis of Nano Sulfur Particles and their Antitumor Activity","authors":"Z. Faten, H. Mustafa, M. Ald","doi":"10.4172/1948-5948.1000397","DOIUrl":"https://doi.org/10.4172/1948-5948.1000397","url":null,"abstract":"Objectives: The present study is designed to evaluate the antitumor activity of sulphur containing compounds in Nano formulations against Ehrlisch Ascites Carcinoma (EAC) in female albino mice. Methods: Preparation of sulfur nanoparticles (S-NPs) was done throughout. Firstly, the evaluation of their composition and purity by fourier transform infrared spectrometer (FT-IR) analysis. Secondly, the shape and size of S-NPs were investigated by scanning electron microscopy (SEM) techniques in all S-NPs samples which were prepared with Tetramethylammoniumbromide (TMAB) surfactant, Then, Energy-dispersive X-ray spectroscopy (EDS) for the evaluation of their composition and purity, after that the X-Ray diffraction patterns were measured for prepared S-NPs with TMAB surfactant, and the determination of S-NPs antitumor activity against (breast carcinoma cell line (MCF7), liver carcinoma cell line (HEPG2), colon carcinoma cell line (HCT116), prostatic carcinoma cell line (PC3). Finally, determination biochemical assay of S-NPs by using female mice bearing EAC and of the assessment of liver functions and evaluation of kidney functions and determination of some heart functions in serum and hematology markers. Results: This study revealed that S-NPs showed high strong activities in concentration (5 mg/kg) as it improvement of liver functions production compared to the positive control group, also showed the effect of S-NPs of the kidney functions, heart functions and hematology parameters in all studied groups and a significant increase in activities compared with positive control group. Conclusion: S-NPs play an important role in improving liver functions, kidney functions and the heart functions of resulted in significantly increase activity against tumour and plays an important role in improving oxidative stress resulted in high antiproliferation activity against (MCF-7, HepG2, HCT116 and PC3) cell lines, and significantly increase with S-NPs reduced most of the hematological parameters towards the best compared to the positive control group.","PeriodicalId":16453,"journal":{"name":"Journal of Microbial & Biochemical Technology","volume":"71 1","pages":"59-68"},"PeriodicalIF":0.0,"publicationDate":"2018-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90369839","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-07-27DOI: 10.4172/1948-5948-C2-039
J. Alderete
{"title":"Approach toward developing perfect serodiagnostic target using Trichomonas vaginalis as a model","authors":"J. Alderete","doi":"10.4172/1948-5948-C2-039","DOIUrl":"https://doi.org/10.4172/1948-5948-C2-039","url":null,"abstract":"","PeriodicalId":16453,"journal":{"name":"Journal of Microbial & Biochemical Technology","volume":"138 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-07-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79779704","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-06-05DOI: 10.4172/1948-5948-C1-037
P. A. Kessler
{"title":"Role of regulatory T cells in mother to child transmission of HIV","authors":"P. A. Kessler","doi":"10.4172/1948-5948-C1-037","DOIUrl":"https://doi.org/10.4172/1948-5948-C1-037","url":null,"abstract":"","PeriodicalId":16453,"journal":{"name":"Journal of Microbial & Biochemical Technology","volume":"57 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-06-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78731638","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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