Pub Date : 2003-01-27DOI: 10.1002/3527600035.BPOL8005
C. Karatzas, N. Chrétien, F. Duguay, Annie Bellemare, J. Zhou, A. Rodenhiser, Shafiul Islam, C. Turcotte, Yue-Jin Huang, A. Lazaris
Introduction Historical Outline Production of rc-Spider Silk Proteins Over-expression of Soluble rc-Spider silk Proteins in Mammalian Cells Secretion of rc-Dragline Spider Silk proteins Large-scale Production of rc-Spider Silk Proteins Purification and Characterization of rc-Spider Silk Proteins Production of rc-Spider Silk Proteins in the Milk of Transgenic Animals Generation of Transgenic Mice for the Spider Dragline Silk Genes Mammary Gland Production of Spider Dragline Silk Proteins Recombinant Spider Silk-based Fiber Spinning Spin Dope Preparation, Fiber Spinning and Characterization Fiber Post-Spinning Treatment Perspective on the Formation of Silk Fibers and the Spinning Process Applications of rc-Spider Silk Fibers, and Prospects Patents Chronological Summary of Patents Conclusions Acknowledgments Keywords: spider silk; genetic engineering; recombinant silk protein; fiber spinning; expression; mammalian cells; transgenic mice; spin dope; fibers
{"title":"High‐toughness Spider Silk Fibers Spun from Soluble Recombinant Silk Produced in Mammalian Cells","authors":"C. Karatzas, N. Chrétien, F. Duguay, Annie Bellemare, J. Zhou, A. Rodenhiser, Shafiul Islam, C. Turcotte, Yue-Jin Huang, A. Lazaris","doi":"10.1002/3527600035.BPOL8005","DOIUrl":"https://doi.org/10.1002/3527600035.BPOL8005","url":null,"abstract":"Introduction \u0000Historical Outline \u0000Production of rc-Spider Silk Proteins \u0000Over-expression of Soluble rc-Spider silk Proteins in Mammalian Cells \u0000Secretion of rc-Dragline Spider Silk proteins \u0000Large-scale Production of rc-Spider Silk Proteins \u0000Purification and Characterization of rc-Spider Silk Proteins \u0000 \u0000 \u0000Production of rc-Spider Silk Proteins in the Milk of Transgenic Animals \u0000Generation of Transgenic Mice for the Spider Dragline Silk Genes \u0000Mammary Gland Production of Spider Dragline Silk Proteins \u0000 \u0000 \u0000Recombinant Spider Silk-based Fiber Spinning \u0000Spin Dope Preparation, Fiber Spinning and Characterization \u0000Fiber Post-Spinning Treatment \u0000Perspective on the Formation of Silk Fibers and the Spinning Process \u0000 \u0000 \u0000Applications of rc-Spider Silk Fibers, and Prospects \u0000Patents \u0000Chronological Summary of Patents \u0000 \u0000 \u0000Conclusions \u0000Acknowledgments \u0000 \u0000 \u0000Keywords: \u0000 \u0000spider silk; \u0000genetic engineering; \u0000recombinant silk protein; \u0000fiber spinning; \u0000expression; \u0000mammalian cells; \u0000transgenic mice; \u0000spin dope; \u0000fibers","PeriodicalId":165163,"journal":{"name":"Biopolymers Online","volume":"26 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2003-01-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"132476998","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2003-01-27DOI: 10.1002/3527600035.BPOL8007
H. Zahn, K. Schäfer, C. Popescu
Introduction Historical Outline Cell Biology The Wool Follicle Expression of Keratin and KAP Genes in Wool Follicle Differentiations Occurrence Sheep Rearing and Breeding Places of Origin and Types of Raw Wool Wool Fiber Geometry Morphology Structural Biology Bonding and Interactions in Wool Fibers α- and β-Keratin Structures in the Cortical Microfibrils Chemical Composition of Wool and its Morphological Components Proteins Free Internal Lipids Covalently Bound Lipids Chemical Analysis Physical and Mechanical Properties Water Absorption Mechanical Properties Glass Transition Thermal Denaturation Wool as a Polyampholyte Chemical Reactivity Dry Heat Moist Heat Acid Degradation Degradation by Alkali Reducing Agents Photodegradation Clothing Physiology Biochemistry Isolation of Wool Proteins Amino Acid Sequences of Wool Proteins Morphological Location of Wool Proteins Biodegradation Enzymatic Degradation Molecular Genetics Production Wool Collection Raw Wool Scouring Mechanical Processing Chemical Processing World Market and Prices Outlook Acknowledgments Keywords: wool; animal fiber; sheep; protein fiber; keratin; morphology; cuticle; cortex; protein chemistry; amino acids; internal lipids; resistant membranes; mechanical properties; water absorption; textiles; production; finishing; world market
Pub Date : 2003-01-27DOI: 10.1002/3527600035.BPOL8014
C. M. Vaz, L. D. Graaf, W. J. Mulder
Introduction Historical Outline Industrial Proteins Types of Industrial Proteins Protein Properties Protein Modifications Protein Processing Techniques Applications of Industrial Proteins Adhesives Coatings Surfactants Bioplastics Controlled-release Systems Outlook and Perspectives Keywords: industrial proteins; adhesives; coatings; surfactants; bioplastics; controlled release
Pub Date : 2003-01-27DOI: 10.1002/3527600035.BPOL8002
F. Vollrath, D. Knight
{"title":"Biology and Technology of Silk Production","authors":"F. Vollrath, D. Knight","doi":"10.1002/3527600035.BPOL8002","DOIUrl":"https://doi.org/10.1002/3527600035.BPOL8002","url":null,"abstract":"","PeriodicalId":165163,"journal":{"name":"Biopolymers Online","volume":"33 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2003-01-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115815295","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2003-01-27DOI: 10.1002/3527600035.BPOL8010
S. Clemens, C. Simm, T. Maier
Introduction Historical Outline Chemical Structures Nomenclature and Structure of Metallothioneins Phytochelatins and Phytochelatin–Metal Complexes Structural Properties of Metallochaperones Chemical Analysis and Detection Metallothioneins Phytochelatins Occurrence Metallothioneins Phytochelatins Metallochaperones Functions Metal Homeostasis and the Role of Metallochaperones Buffering and Detoxification Physiology Metallothionein Localization and Isoforms Localization and Compartmentation of Phytochelatin Synthesis Biochemistry Metal-binding Characteristics of Metallothioneins Biochemistry of Phytochelatin Synthesis Molecular Genetics Metallothionein Genes and Their Regulation Phytochelatin Synthase Genes Biotechnological Applications Patents Outlook and Perspectives Keywords: metallothioneins; phytochelatins; metallochaperones; glutathione; cofactor; heavy metal; heavy metal tolerance; heavy metal accumulation; heavy metal toxicity; phytoremediation; metal trafficking; metal transport; Alzheimer's disease; prion diseases; autism; zinc; iron; copper; cadmium
Pub Date : 2003-01-27DOI: 10.1002/3527600035.BPOL8016
A. McPherson, R. Cudney, Sam Patel
Introduction Solubility and Supersaturation Properties of Macromolecular Crystals Preparation of Samples Precipitants and Their Effects The Use of Temperature for Crystallization Creating Supersaturation – Methodology Factors Affecting Crystallization Screening and Optimization Theoretical Contributions to Macromolecular Crystal Growth Future Trends
{"title":"Crystallization of Proteins, Nucleic Acids, and Viruses for X‐ray Diffraction Analysis","authors":"A. McPherson, R. Cudney, Sam Patel","doi":"10.1002/3527600035.BPOL8016","DOIUrl":"https://doi.org/10.1002/3527600035.BPOL8016","url":null,"abstract":"Introduction \u0000Solubility and Supersaturation \u0000Properties of Macromolecular Crystals \u0000Preparation of Samples \u0000Precipitants and Their Effects \u0000The Use of Temperature for Crystallization \u0000Creating Supersaturation – Methodology \u0000Factors Affecting Crystallization \u0000Screening and Optimization \u0000Theoretical Contributions to Macromolecular Crystal Growth \u0000Future Trends","PeriodicalId":165163,"journal":{"name":"Biopolymers Online","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2003-01-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130828977","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2003-01-27DOI: 10.1002/3527600035.BPOL8017
D. Aceti, Paul G. Blommel, Y. Endo, B. Fox, R. Frederick, A. Hegeman, W. Jeon, Todd Kimball, Jason M. Lee, Craig S. Newman, F. Peterson, T. Sawasaki, K. Seder, M. Sussman, E. Ulrich, R. Wrobel, S. Thao, D. Vinarov, B. Volkman, Qin Zhao
Introduction Overview of Structural Genomics Initiatives World-wide Use of Bioinformatics as a Predictive Tool High-throughput Cloning Strategies Applied to Arabidopsis cDNA Sources DNA Chip Technologies as a Predictive Tool Genes Lacking Introns Restriction Digestion and Ligation Topoisomerase Cloning Transformation Sequence Verification Escherichia coli Expression Systems T7 Promoter Systems Arabinose Operon Promoter System Wheat Germ Cell-free Translation Systems Characterization of Expression, Solubility, and Purity Electrophoresis and Dye Binding ELISA-based Detection S-Tag-Based Detection Protein Tags and Multi-step Purification Procedures Role of Protease Cleavage in Target Preparation Protein Characterization by Mass Spectrometry Integration with Broader Biological Sciences Community Acknowledgments Keywords: affinity tag; Arabidopsis thaliana; bioinformatics; catabolite repression; codon bias; codon usage; Escherichia coli; expression vectors; factor Xa; fusion protein; gene expression; heterologous expression; high-density oligonucleotide arrays; Laboratory Information Management Systems; MALDI-TOF; mass spectrometry; peptide mapping; PreScission; protease cleavage; protein sequence analysis; solubility tag; soluble protein; structure and functional biology; TEV; T7 bacteriophage promoter; T7 RNA polymerase; target selection; thrombin; transcriptome
{"title":"Role of Nucleic Acid and Protein Manipulation Technologies in High-throughput Structural Biology Efforts","authors":"D. Aceti, Paul G. Blommel, Y. Endo, B. Fox, R. Frederick, A. Hegeman, W. Jeon, Todd Kimball, Jason M. Lee, Craig S. Newman, F. Peterson, T. Sawasaki, K. Seder, M. Sussman, E. Ulrich, R. Wrobel, S. Thao, D. Vinarov, B. Volkman, Qin Zhao","doi":"10.1002/3527600035.BPOL8017","DOIUrl":"https://doi.org/10.1002/3527600035.BPOL8017","url":null,"abstract":"Introduction \u0000Overview of Structural Genomics Initiatives World-wide \u0000Use of Bioinformatics as a Predictive Tool \u0000High-throughput Cloning Strategies Applied to Arabidopsis \u0000cDNA Sources \u0000DNA Chip Technologies as a Predictive Tool \u0000Genes Lacking Introns \u0000Restriction Digestion and Ligation \u0000Topoisomerase Cloning \u0000Transformation \u0000Sequence Verification \u0000 \u0000 \u0000Escherichia coli Expression Systems \u0000T7 Promoter Systems \u0000Arabinose Operon Promoter System \u0000 \u0000 \u0000Wheat Germ Cell-free Translation Systems \u0000Characterization of Expression, Solubility, and Purity \u0000Electrophoresis and Dye Binding \u0000ELISA-based Detection \u0000S-Tag-Based Detection \u0000 \u0000 \u0000Protein Tags and Multi-step Purification Procedures \u0000Role of Protease Cleavage in Target Preparation \u0000Protein Characterization by Mass Spectrometry \u0000Integration with Broader Biological Sciences Community \u0000Acknowledgments \u0000 \u0000 \u0000Keywords: \u0000 \u0000affinity tag; \u0000Arabidopsis thaliana; \u0000bioinformatics; \u0000catabolite repression; \u0000codon bias; \u0000codon usage; \u0000Escherichia coli; \u0000expression vectors; \u0000factor Xa; \u0000fusion protein; \u0000gene expression; \u0000heterologous expression; \u0000high-density oligonucleotide arrays; \u0000Laboratory Information Management Systems; \u0000MALDI-TOF; \u0000mass spectrometry; \u0000peptide mapping; \u0000PreScission; \u0000protease cleavage; \u0000protein sequence analysis; \u0000solubility tag; \u0000soluble protein; \u0000structure and functional biology; \u0000TEV; \u0000T7 bacteriophage promoter; \u0000T7 RNA polymerase; \u0000target selection; \u0000thrombin; \u0000transcriptome","PeriodicalId":165163,"journal":{"name":"Biopolymers Online","volume":"29 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2003-01-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121119849","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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