Pub Date : 2018-01-01DOI: 10.14480/JM.2018.16.3.147
김용균, 이병주, 이순계, Lee Byung Eui
{"title":"Study on new casing materials of Agaricus bisporus","authors":"김용균, 이병주, 이순계, Lee Byung Eui","doi":"10.14480/JM.2018.16.3.147","DOIUrl":"https://doi.org/10.14480/JM.2018.16.3.147","url":null,"abstract":"","PeriodicalId":16539,"journal":{"name":"Journal of Mushroom","volume":"71 1","pages":"147-154"},"PeriodicalIF":0.0,"publicationDate":"2018-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75468227","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-01-01DOI: 10.14480/JM.2018.16.4.267
이관우, Jong-Ok Jeon, 김민자, 김익제, Jang myoung jun, 박혜성
{"title":"Effects of difference in medium composition on the growth of Lentinula edodes","authors":"이관우, Jong-Ok Jeon, 김민자, 김익제, Jang myoung jun, 박혜성","doi":"10.14480/JM.2018.16.4.267","DOIUrl":"https://doi.org/10.14480/JM.2018.16.4.267","url":null,"abstract":"","PeriodicalId":16539,"journal":{"name":"Journal of Mushroom","volume":"31 1","pages":"267-271"},"PeriodicalIF":0.0,"publicationDate":"2018-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84409156","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-01-01DOI: 10.14480/JM.2018.16.3.208
김경제, Seung-bin Im, 김소망, Park Jun Ki, Seo-Hyun Lee, Jung-Beom Kim
{"title":"Comparison of the proximate composition and amino acid content of domestic and imported Tricholoma matsutake and Sarcodon aspratus","authors":"김경제, Seung-bin Im, 김소망, Park Jun Ki, Seo-Hyun Lee, Jung-Beom Kim","doi":"10.14480/JM.2018.16.3.208","DOIUrl":"https://doi.org/10.14480/JM.2018.16.3.208","url":null,"abstract":"","PeriodicalId":16539,"journal":{"name":"Journal of Mushroom","volume":"16 1","pages":"208-212"},"PeriodicalIF":0.0,"publicationDate":"2018-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81384807","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-01-01DOI: 10.14480/JM.2018.16.1.31
Hong-Sin Park, Y. Yeom, Min-ho Yoon
{"title":"Comparison on phosphate solubilization ability of Pantoea rodasil and Burkholderia stabilis isolated from button mushroom media","authors":"Hong-Sin Park, Y. Yeom, Min-ho Yoon","doi":"10.14480/JM.2018.16.1.31","DOIUrl":"https://doi.org/10.14480/JM.2018.16.1.31","url":null,"abstract":"","PeriodicalId":16539,"journal":{"name":"Journal of Mushroom","volume":"3 1","pages":"31-38"},"PeriodicalIF":0.0,"publicationDate":"2018-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84272072","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2017-12-31DOI: 10.14480/JM.2017.15.4.244
최영진, 범미기, 최은주, 김은경
In this study, the inhibitory effect of Paecilomyces tenuipes extract on PSA and angiogenesis-related factor expression levels were investigated in human prostate cancer cells, LNCaP. P. tenuipes extract significantly inhibited PSA expression in a dose-dependent manner. We also investigated the inhibitory effect of P. tenuipes extract on the expression of angiogenesis-related genes including VEGF, MMP-2, MMP-9, TIMP-1, and TIMP-2. P. tenuipes extract significantly down-regulated the expression of MMP-2 and MMP-9 in a dose-dependent manner. On the contrary, P. tenuipes increased the expression of TIMP-1 and TIMP-2. Our findings indicate that P. tenuipes exhibits an inhibitory effect on angiogenesis in human prostate cancer cells.
{"title":"Effect of Paecilomyces tenuipes extract on angiogenesis in prostate cancer cells","authors":"최영진, 범미기, 최은주, 김은경","doi":"10.14480/JM.2017.15.4.244","DOIUrl":"https://doi.org/10.14480/JM.2017.15.4.244","url":null,"abstract":"In this study, the inhibitory effect of Paecilomyces tenuipes extract on PSA and angiogenesis-related factor expression levels were investigated in human prostate cancer cells, LNCaP. P. tenuipes extract significantly inhibited PSA expression in a dose-dependent manner. We also investigated the inhibitory effect of P. tenuipes extract on the expression of angiogenesis-related genes including VEGF, MMP-2, MMP-9, TIMP-1, and TIMP-2. P. tenuipes extract significantly down-regulated the expression of MMP-2 and MMP-9 in a dose-dependent manner. On the contrary, P. tenuipes increased the expression of TIMP-1 and TIMP-2. Our findings indicate that P. tenuipes exhibits an inhibitory effect on angiogenesis in human prostate cancer cells.","PeriodicalId":16539,"journal":{"name":"Journal of Mushroom","volume":"13 1","pages":"244-248"},"PeriodicalIF":0.0,"publicationDate":"2017-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85445867","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2017-09-30DOI: 10.14480/JM.2017.15.3.111
이병주, 이미애, 김용균, 이순계, 최영상, 이병의
The mushroom species Cordyceps militaris has been studied and cultivated as a medicinal mushroom due to its multiple valuable biological and pharmaceutical activities. For breeding new strains of C. militaris, multiplex PCR assays were performed using primers specific for its mating type genes, MAT1-1 and MAT1-2. Mating types and mating status were confirmed, as evidenced by DNA bands at 233-bp and 191-bp for MAT1-1 and MAT1-2 respectively. The novel 'Dowonhongcho 2ho’ was developed through mating; they were found to possess high-quality fruiting bodies when grown in artificial media. The stromata of the new strain were club-shaped, with a bright orange-red color, and measured 7.1 cm in length. They had an average cordycepin content of 0.33%. Compared to 'Dowonhongcho,' the new strain had a 7% higher yield, as well as firm fruiting bodies. The optimum temperature for mycelial growth was 20~25°C, and the optimum temperature for stroma development was 18~22 °C. The fruiting bodies developed after 49.1 days from inoculation. The use of mating type molecular markers improved the breeding efficiency of the new strain 'Dowonhongcho 2ho.’ Thus, they may be valuable for artificial cultivation and industrial-scale production of C. militaris with excellent characteristics.
{"title":"교배형 분자마커를 이용한 신품종 밀리타리스 동충하초 ‘도원홍초 2호’의 품종 특성","authors":"이병주, 이미애, 김용균, 이순계, 최영상, 이병의","doi":"10.14480/JM.2017.15.3.111","DOIUrl":"https://doi.org/10.14480/JM.2017.15.3.111","url":null,"abstract":"The mushroom species Cordyceps militaris has been studied and cultivated as a medicinal mushroom due to its multiple valuable biological and pharmaceutical activities. For breeding new strains of C. militaris, multiplex PCR assays were performed using primers specific for its mating type genes, MAT1-1 and MAT1-2. Mating types and mating status were confirmed, as evidenced by DNA bands at 233-bp and 191-bp for MAT1-1 and MAT1-2 respectively. The novel 'Dowonhongcho 2ho’ was developed through mating; they were found to possess high-quality fruiting bodies when grown in artificial media. The stromata of the new strain were club-shaped, with a bright orange-red color, and measured 7.1 cm in length. They had an average cordycepin content of 0.33%. Compared to 'Dowonhongcho,' the new strain had a 7% higher yield, as well as firm fruiting bodies. The optimum temperature for mycelial growth was 20~25°C, and the optimum temperature for stroma development was 18~22 °C. The fruiting bodies developed after 49.1 days from inoculation. The use of mating type molecular markers improved the breeding efficiency of the new strain 'Dowonhongcho 2ho.’ Thus, they may be valuable for artificial cultivation and industrial-scale production of C. militaris with excellent characteristics.","PeriodicalId":16539,"journal":{"name":"Journal of Mushroom","volume":"252 1","pages":"111-117"},"PeriodicalIF":0.0,"publicationDate":"2017-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79622545","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2017-03-31DOI: 10.14480/JM.2017.15.1.25
Sung-I Woo, W. Kong, K. Jang
: ‘Baekseung’, a new variety of Flammulina velutipes , was bred by mating two monokaryotic strains isolated from KMCC 4210 and KMCC 4216 in the Mushroom Research Division, Baekseung ARES in 2016. The Baekseung and Uri1ho strains showed fast mycelial growth and mycelial density on malt extract agar media after 7 days of incubation. The spawn running period on the sawdust substrate required a cultivation period and temperature of 30 days and 25 o C, respectively, for primordia formation where in fruiting body development occurred from 11±1 days at 14 o C and 14±1 days at 7 o C. The length of the pilei and stipes of the Baekseung harvested in optimal stag were 11.3±0.4 and 89.2±7.1 mm, respectively, whereas the values for Uri1ho were 10.7±1.0 and 91.3±20.8 mm, respectively. The yield of the Baekseung and Uri1ho strain grown on the sawdust substrate was 153.7±12.5 and 139.8±17.8 g, respectively, per 850 ml in bottle cultivation. The inferred tree exhibited a phylogenetic relationship between the Korean white fruiting body strains of Baekseung, Uri1ho and Fv-14-a-38, Fv-14-a-51, and the Japanese white fruiting body-forming strains of KMCC 4226, and these were confirmed to be genetically related.
摘要:‘百胜’是2016年由白城菌类研究中心分离的KMCC 4210和KMCC 4216两株单核菌株杂交而成的金针菇新品种。培养7 d后，Baekseung和Uri1ho菌株在麦芽提取物琼脂培养基上菌丝生长速度快，菌丝密度高。在木屑基质上产卵需要30天的培养时间和25℃的温度，在14℃和7℃的条件下，原基形成需要11±1天，子实体发育需要14±1天。在最佳雄雄期收获的百胜菌的毛长和柄长分别为11.3±0.4和89.2±7.1 mm，而在优雄雄期收获的百胜菌的毛长和柄长分别为10.7±1.0和91.3±20.8 mm。在木屑基质上培养的Baekseung和Uri1ho菌株的瓶培养产量分别为153.7±12.5 g / 850 ml和139.8±17.8 g / 850 ml。韩国白子实体株系Baekseung、Uri1ho和Fv-14-a-38、Fv-14-a-51与日本白子实体形成株系KMCC 4226之间存在亲缘关系。
{"title":"Characteristics of 'Baekseung', a new cultivar Flammulina velutipes","authors":"Sung-I Woo, W. Kong, K. Jang","doi":"10.14480/JM.2017.15.1.25","DOIUrl":"https://doi.org/10.14480/JM.2017.15.1.25","url":null,"abstract":": ‘Baekseung’, a new variety of Flammulina velutipes , was bred by mating two monokaryotic strains isolated from KMCC 4210 and KMCC 4216 in the Mushroom Research Division, Baekseung ARES in 2016. The Baekseung and Uri1ho strains showed fast mycelial growth and mycelial density on malt extract agar media after 7 days of incubation. The spawn running period on the sawdust substrate required a cultivation period and temperature of 30 days and 25 o C, respectively, for primordia formation where in fruiting body development occurred from 11±1 days at 14 o C and 14±1 days at 7 o C. The length of the pilei and stipes of the Baekseung harvested in optimal stag were 11.3±0.4 and 89.2±7.1 mm, respectively, whereas the values for Uri1ho were 10.7±1.0 and 91.3±20.8 mm, respectively. The yield of the Baekseung and Uri1ho strain grown on the sawdust substrate was 153.7±12.5 and 139.8±17.8 g, respectively, per 850 ml in bottle cultivation. The inferred tree exhibited a phylogenetic relationship between the Korean white fruiting body strains of Baekseung, Uri1ho and Fv-14-a-38, Fv-14-a-51, and the Japanese white fruiting body-forming strains of KMCC 4226, and these were confirmed to be genetically related.","PeriodicalId":16539,"journal":{"name":"Journal of Mushroom","volume":"1 1","pages":"25-30"},"PeriodicalIF":0.0,"publicationDate":"2017-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75404356","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
An auxin-producing bacterial strain, designated 4-3, was isolated from waste button mushroom compost in Boryeong-si, Chungnam. The strain 4-3 was classified as a novel strain of Leucobacter tardus, based on chemotaxonomic and phylogenetic analyses. TLC and HPLC the isolated L. tardus strain 4-3 produced indole-3-acetic acid (IAA), the auxin. Maximum IAA productionof 94.3 mg L-1 was detected for bacteria cultured in R2A medium with 0.1% l-tryptophan, incubated for 24 h at 35oC. Negative correlationwas observed between IAA production and pH of the culture medium, indicating that the increase inIAA caused acidification ofthe medium. The effect of supplementation with varying concentrations of l-tryptophan, a known precursor of IAA, was also assessed. production was maximal at 0.1% l, but decreased at lconcentrations above 0.2%. To investigate the plant growth-promoting effects of the bacterium, L. tardus strain 4-3 culture broth was used to inoculate water cultures and seed pots of mung bean. We found thatadventitious root induction and root growth were 2.2-times higher in thethan in the non-inoculated plants.
{"title":"Characterization of auxin production plant growth promotion by a bacterium isolated from button mushroom compost","authors":"Jisu Yoo, Heon-Hak Lee, Chang-Hoon Han, Min-ho Yoon","doi":"10.14480/JM.2017.15.1.8","DOIUrl":"https://doi.org/10.14480/JM.2017.15.1.8","url":null,"abstract":"An auxin-producing bacterial strain, designated 4-3, was isolated from waste button mushroom compost in Boryeong-si, Chungnam. The strain 4-3 was classified as a novel strain of Leucobacter tardus, based on chemotaxonomic and phylogenetic analyses. TLC and HPLC the isolated L. tardus strain 4-3 produced indole-3-acetic acid (IAA), the auxin. Maximum IAA productionof 94.3 mg L-1 was detected for bacteria cultured in R2A medium with 0.1% l-tryptophan, incubated for 24 h at 35oC. Negative correlationwas observed between IAA production and pH of the culture medium, indicating that the increase inIAA caused acidification ofthe medium. The effect of supplementation with varying concentrations of l-tryptophan, a known precursor of IAA, was also assessed. production was maximal at 0.1% l, but decreased at lconcentrations above 0.2%. To investigate the plant growth-promoting effects of the bacterium, L. tardus strain 4-3 culture broth was used to inoculate water cultures and seed pots of mung bean. We found thatadventitious root induction and root growth were 2.2-times higher in thethan in the non-inoculated plants.","PeriodicalId":16539,"journal":{"name":"Journal of Mushroom","volume":"26 1","pages":"8-13"},"PeriodicalIF":0.0,"publicationDate":"2017-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88712063","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2017-03-31DOI: 10.14480/JM.2017.15.1.54
W. Jo, Sung-Hak Lee, J. Koo, Song-Yi Ryu, Min-gu Kang, Soon-Young Lim, Seung-Chun Park
Wolfiporia cocos is a well-known traditional medicine in China, Japan, Korea, and other Asian countries owing to its numerous therapeutic properties. With the aim to determine the morphology and genetic characteristics of W. cocosten strains of W. cocos were cultivated in vitro, and subsequently, rapid amplification of polymorphic DNA was performed. To the best of our knowledge, this is the first study to examine the morphology of fruit bodies of W. cocos in Korea. W. cocos were cultured on PDA agar at different temperatures (12, 16, 20, 24, and 28oC) under 12-hour light (600 Lux) / 12-hour dark photoperiod condition for 1 month. Appearance of fruit body was the highest at 28°C condition in all the strains investigated. Honeycomb-like structure on sclerotia was observed in Andong 01, Andong 02, Andong 03, KFRI 1104, KFRI 1105, KFRI 1106, KFRI 1107, KFRI 1108, and ASI 13007 strains of . The KFRI 1103 strain formed cosmos petal-like structure on sclerotia. The average size of basidiospores was recorded as 7.55 μm in height and 3.35 μ in width.
{"title":"Morphological characteristics of fruit bodies and basidiospores of Wolfiporia extensa","authors":"W. Jo, Sung-Hak Lee, J. Koo, Song-Yi Ryu, Min-gu Kang, Soon-Young Lim, Seung-Chun Park","doi":"10.14480/JM.2017.15.1.54","DOIUrl":"https://doi.org/10.14480/JM.2017.15.1.54","url":null,"abstract":"Wolfiporia cocos is a well-known traditional medicine in China, Japan, Korea, and other Asian countries owing to its numerous therapeutic properties. With the aim to determine the morphology and genetic characteristics of W. cocosten strains of W. cocos were cultivated in vitro, and subsequently, rapid amplification of polymorphic DNA was performed. To the best of our knowledge, this is the first study to examine the morphology of fruit bodies of W. cocos in Korea. W. cocos were cultured on PDA agar at different temperatures (12, 16, 20, 24, and 28oC) under 12-hour light (600 Lux) / 12-hour dark photoperiod condition for 1 month. Appearance of fruit body was the highest at 28°C condition in all the strains investigated. Honeycomb-like structure on sclerotia was observed in Andong 01, Andong 02, Andong 03, KFRI 1104, KFRI 1105, KFRI 1106, KFRI 1107, KFRI 1108, and ASI 13007 strains of . The KFRI 1103 strain formed cosmos petal-like structure on sclerotia. The average size of basidiospores was recorded as 7.55 μm in height and 3.35 μ in width.","PeriodicalId":16539,"journal":{"name":"Journal of Mushroom","volume":"19 1","pages":"54-56"},"PeriodicalIF":0.0,"publicationDate":"2017-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82051698","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2017-03-31DOI: 10.14480/JM.2017.15.1.57
오태석, 이윤혜, 김창호, 조용구, 장명준
In this study, we attempted to find alternative materials to rice bran (Japonica in Korea) such as rice bran pellet (Indica in Pakistan), corn distillers dried grains with solubles, sesame oil meal, and kapok meal for bottle cultivation of Pleurotus eryngii. Among all treatments, the total carbon content of the mixed substrate was the highest in T1 and the total nitrogen content was the highest in T4. The C/N ratio of T2 was comparable to that of the control and was higher than that of other treatments. The diameter of the pileus was the largest in T1 and T2, and the length of the stipe was the largest in T1 and T2. The yield and bio-efficiency of the fruit body were higher in T2 than in the other treatments, and were similar to that of the control plot. Therefore, the findings of our study suggest that rice bran pellet (Indica in Pakistan) could be a suitable alternative to rice bran (Japonica in Korea) for the cultivation of P. eryngii.
{"title":"미강 대체 배지원료에 따른 큰느타리버섯의 생육특성 비교","authors":"오태석, 이윤혜, 김창호, 조용구, 장명준","doi":"10.14480/JM.2017.15.1.57","DOIUrl":"https://doi.org/10.14480/JM.2017.15.1.57","url":null,"abstract":"In this study, we attempted to find alternative materials to rice bran (Japonica in Korea) such as rice bran pellet (Indica in Pakistan), corn distillers dried grains with solubles, sesame oil meal, and kapok meal for bottle cultivation of Pleurotus eryngii. Among all treatments, the total carbon content of the mixed substrate was the highest in T1 and the total nitrogen content was the highest in T4. The C/N ratio of T2 was comparable to that of the control and was higher than that of other treatments. The diameter of the pileus was the largest in T1 and T2, and the length of the stipe was the largest in T1 and T2. The yield and bio-efficiency of the fruit body were higher in T2 than in the other treatments, and were similar to that of the control plot. Therefore, the findings of our study suggest that rice bran pellet (Indica in Pakistan) could be a suitable alternative to rice bran (Japonica in Korea) for the cultivation of P. eryngii.","PeriodicalId":16539,"journal":{"name":"Journal of Mushroom","volume":"312 1","pages":"57-60"},"PeriodicalIF":0.0,"publicationDate":"2017-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75448127","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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