Journal of Radiation Research and Applied Sciences最新文献_第7页

Regulation of the LAMTOR5-AS1/miR-134-5p/ANGPTL4 axis by ING5 in non-small-cell lung cancer ING5在非小细胞肺癌中对LAMTOR5-AS1/miR-134-5p/ANGPTL4轴的调控

IF 2.5 4区综合性期刊 Q2 MULTIDISCIPLINARY SCIENCES

Journal of Radiation Research and Applied Sciences

Pub Date : 2025-12-23 DOI: 10.1016/j.jrras.2025.102143

Haihua Zhang , Xueying Liu , Xinli Liu , Yifan Zhang , Guizhou Gao , Xiaodong Wang , Lin Qu , Gaofeng Liu , Tao Zhang

Background

The underlying mechanisms of non-small-cell lung cancer (NSCLC) are still not fully understood. Inhibitor of growth 5 (ING5) overexpression inhibit aggressiveness of NSCLC.

Methods

The analysis of differentially expressed mRNAs and lncRNAs was conducted using whole transcriptome sequencing. miRNA microarray revealed differentially expressed miRNAs. LncACTdb 3.0 and miRTarBase were used to construct ceRNA interaction network. qRT-PCR was performed to validate gene expression levels. Single-cell RNA sequencing analysis was performed to analysis tumor immune infiltration. Dual-luciferase reporter assay was used to verify the combination. Quantitative real-time PCR and Western blot were performed to validate LAMTOR5-AS1, miR-134-5p, and ANGPTL4 expression.

Results

1648 differentially expressed mRNAs (833 up- and 815 downregulated-) between the control and ING5 overexpression groups; further, 114 immune-related mRNAs were observed to be differentially expressed. Overall, 110 lncRNAs were found to interact with 15 differential miRNAs, resulting in 230 potential interactions. Survival and expression analyses led to the identification of the LAMTOR5-AS1/miR-134-5p/ANGPTL4 axis as a potential regulatory pathway. Upregulated ANGPTL4 expression was associated with poor prognosis with increased numbers of fibroblasts and decreased numbers of T cells. miR-134-5p was the target gene of LAMTOR5-AS1. Furthermore, the decrease in miR-134-5p expression partially reversed the inhibitory impact of LAMTOR5-AS1 depletion on cell proliferation, migration, and invasion.

Conclusion

To conclude, the novel LAMTOR5-AS1/miR-134-5p/ANGPTL4 axis inhibits NSCLC progression by regulating immune infiltration in ING5 overexpression cells.

非小细胞肺癌（NSCLC）的发病机制尚不完全清楚。生长抑制因子5 （ING5）过表达抑制NSCLC侵袭性。方法采用全转录组测序对差异表达mrna和lncrna进行分析。miRNA微阵列显示差异表达的miRNA。使用LncACTdb 3.0和miRTarBase构建ceRNA交互网络。采用qRT-PCR验证基因表达水平。单细胞RNA测序分析肿瘤免疫浸润。双荧光素酶报告试验验证联合。采用实时荧光定量PCR和Western blot验证LAMTOR5-AS1、miR-134-5p和ANGPTL4的表达。结果对照组和ING5过表达组有1648个mrna差异表达（上调833个，下调815个）；此外，114种免疫相关mrna被观察到差异表达。总体而言，110个lncrna被发现与15个差异mirna相互作用，导致230个潜在的相互作用。生存和表达分析发现LAMTOR5-AS1/miR-134-5p/ANGPTL4轴是一个潜在的调控途径。ANGPTL4表达上调与成纤维细胞数量增加、T细胞数量减少的不良预后相关。miR-134-5p是LAMTOR5-AS1的靶基因。此外，miR-134-5p表达的降低部分逆转了LAMTOR5-AS1缺失对细胞增殖、迁移和侵袭的抑制作用。综上所述，新的LAMTOR5-AS1/miR-134-5p/ANGPTL4轴通过调节ING5过表达细胞的免疫浸润来抑制NSCLC的进展。

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引用次数: 0

Weifuchun alleviates MNNG-induced gastric epithelial cell injury via TGF-β1/PI3K/Akt pathway 胃复春通过TGF-β1/PI3K/Akt通路减轻mnng诱导的胃上皮细胞损伤

IF 2.5 4区综合性期刊 Q2 MULTIDISCIPLINARY SCIENCES

Journal of Radiation Research and Applied Sciences

Pub Date : 2025-12-23 DOI: 10.1016/j.jrras.2025.102111

Niansong Kang , Haijun Fang , Sihui Zheng

Background

Chronic atrophic gastritis (CAG), a chronic form of gastritis, is critical in the early stages of stomach precancerous lesions. Delving into the origins of chronic atrophic gastritis and conducting drug screenings is crucial. However, the precise mechanisms by which Weifuchun protects gastric mucosal epithelial cells remain incompletely understood. This project's objective is to investigate the potential mechanisms by which Weifuchun safeguards gastric mucosal epithelial cells from harm.

Methods

We mimicked the CAG process by MNNG-induced injury of gastric mucosal epithelial cells (GES-1) and detected changes in cell activity, apoptosis, cell cycle and migration and invasion. Changes in cellular epithelial-mesenchymal transition (EMT), PI3K/AKT pathway proteins and inflammatory factors were detected. Exploring how Weifuchun influences the biological actions of GES-1 induced by MNNG. Then, pharmacological rescue experiments were performed by treatment with TGF-β1/PI3K/AKT inhibitors.

Results

MNNG induced GES-1 to undergo cellular aggressive transformation, including proliferation, invasion, and EMT. Weifuchun significantly ameliorated MNNG-induced damage to GES-1, with the effects of this treatment being regulated by the TGF-β1 and PI3K/Akt pathways.

Conclusion

The results demonstrated that Weifuchun has the potential to mitigate MNNG-induced inflammatory injury and aggressive transformation of gastric epithelial cells by modulating the TGF-β1/PI3K/AKT pathway. This suggests potential new perspectives and approaches for future research into the prevention and management of gastric cancer.

背景：慢性萎缩性胃炎（CAG）是一种慢性胃炎，在胃癌前病变的早期阶段至关重要。深入研究慢性萎缩性胃炎的起源并进行药物筛选至关重要。然而，胃复春保护胃粘膜上皮细胞的确切机制尚不完全清楚。本项目旨在探讨胃复春保护胃粘膜上皮细胞免受伤害的可能机制。方法通过mnng诱导胃粘膜上皮细胞（GES-1）损伤，模拟CAG过程，检测细胞活性、凋亡、细胞周期、迁移和侵袭的变化。检测细胞上皮-间质转化（EMT）、PI3K/AKT通路蛋白和炎症因子的变化。探讨卫府春对MNNG诱导的GES-1生物活性的影响。然后用TGF-β1/PI3K/AKT抑制剂治疗，进行药理拯救实验。结果smnng诱导GES-1发生细胞侵袭性转化，包括增殖、侵袭和EMT。卫府春可显著改善mnng诱导的GES-1损伤，其作用受TGF-β1和PI3K/Akt通路调控。结论胃复春可能通过调节TGF-β1/PI3K/AKT通路，减轻mnng诱导的胃上皮细胞炎症损伤和侵袭性转化。这为今后胃癌的防治研究提供了新的思路和途径。

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引用次数: 0

An integrated analysis reveals DEFB1, SPINK6 and tumor-suppressive Anaerococcus as prognostic determinants in oral squamous cell carcinoma 一项综合分析显示DEFB1、SPINK6和肿瘤抑制性厌氧球菌是口腔鳞状细胞癌的预后决定因素

IF 2.5 4区综合性期刊 Q2 MULTIDISCIPLINARY SCIENCES

Journal of Radiation Research and Applied Sciences

Pub Date : 2025-12-22 DOI: 10.1016/j.jrras.2025.102117

Yiyun Pan , Kang Liu , Xiaomei Liu , Xiaoming Nie , Keqing Luo , Fuchao Liu , Dechang Xu , Keqiang Tian , Wen Zeng

Background

Oral squamous cell carcinoma (OSCC) is a highly aggressive malignant tumor for which reliable prognostic biomarkers are currently lacking. Growing evidence suggests that the tumor-associated microbiota plays an important role in cancer progression and patient prognosis. However, the prognostic significance of intratumoral microbes in OSCC and their interactions with host genes remain poorly understood.

Methods

This study integrated microbiome and transcriptome data from The Cancer Genome Atlas (TCGA) and employed Cox and LASSO regression to identify survival-associated microbes (SAMs). Microbial–immune interactions were explored through clustering and immune infiltration analyses. Key prognosis-associated microbial genes (PAMGs) were further screened by combining differential gene expression analysis, protein–protein interaction (PPI) network construction, and survival analysis. Functional enrichment analysis was subsequently performed. Experimental validation was conducted using a co-culture system of WSU-HN30 cells and Anaerococcus supernatant.

Results

A total of 63 SAMs were identified, among which Anaerococcus and Peptoniphilus exhibited a significant synergistic relationship. DEFB1 (low expression) and SPINK6 (high expression) were identified as key PAMGs. ROC analysis yielded AUC values of 0.89, 0.85, and 0.82 at 1, 3, and 5 years, respectively, indicating good prognostic predictive ability. Functional analyses indicated that these genes are primarily involved in the cell cycle, epithelial–mesenchymal transition (EMT), and E2F/G2M signaling pathways. In in vitro experiments, Anaerococcus was found to inhibit the proliferation, migration, and invasion of WSU-HN30 cells, accompanied by the upregulation of DEFB1 and down-regulation of SPINK6.

Conclusion

This study uncovers novel microbe–gene interactions in OSCC and identifies DEFB1 and SPINK6 as potential prognostic biomarkers. The results suggest that Anaerococcus may exert a protective role by regulating host gene expression, providing new insights for prognostic stratification and potential therapeutic interventions in OSCC.

背景：口腔鳞状细胞癌是一种高度侵袭性的恶性肿瘤，目前缺乏可靠的预后生物标志物。越来越多的证据表明，肿瘤相关微生物群在癌症进展和患者预后中起着重要作用。然而，肿瘤内微生物在OSCC中的预后意义及其与宿主基因的相互作用仍然知之甚少。方法本研究整合了癌症基因组图谱（TCGA）的微生物组和转录组数据，采用Cox和LASSO回归鉴定存活相关微生物（sam）。通过聚类分析和免疫浸润分析探讨微生物与免疫的相互作用。结合差异基因表达分析、蛋白-蛋白相互作用（PPI）网络构建和生存分析，进一步筛选关键预后相关微生物基因（PAMGs）。随后进行功能富集分析。采用WSU-HN30细胞与厌氧球菌上清液共培养体系进行实验验证。结果共鉴定出63个sam，其中厌氧球菌和嗜胃杆菌表现出显著的协同作用关系。DEFB1（低表达）和SPINK6（高表达）被鉴定为关键PAMGs。ROC分析显示，1年、3年和5年的AUC值分别为0.89、0.85和0.82，表明预后预测能力良好。功能分析表明，这些基因主要参与细胞周期、上皮-间质转化（EMT）和E2F/G2M信号通路。在体外实验中，发现厌氧球菌抑制WSU-HN30细胞的增殖、迁移和侵袭，同时伴有DEFB1上调和SPINK6下调。结论本研究揭示了OSCC中新的微生物-基因相互作用，并确定了DEFB1和SPINK6是潜在的预后生物标志物。结果提示，厌氧球菌可能通过调节宿主基因表达发挥保护作用，为OSCC的预后分层和潜在的治疗干预提供了新的见解。

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引用次数: 0

TID effect study and hardened method of cascode GaN HEMT 级联GaN HEMT的TID效应研究及硬化方法

IF 2.5 4区综合性期刊 Q2 MULTIDISCIPLINARY SCIENCES

Journal of Radiation Research and Applied Sciences

Pub Date : 2025-12-22 DOI: 10.1016/j.jrras.2025.102136

Yanfei Zhang , Xueqin Gong , Yan Wang , Rui Zhao , JinLong Wang , Xiaowu Cai , Mengxin Liu

Conventional cascode GaN HEMTs, which combine a trench gate Si MOSFET with a D-HEMT, are vulnerable to total ionizing dose (TID) radiation, exhibiting severe threshold voltage (V_GS(th)) degradation and functional failure at relatively low dose levels (≤30 krad(Si)). In this paper, a TID-hardened cascode GaN HEMT is proposed by integrating a radiation-hardened Si MOSFET with a commercial D-HEMT. By replacing the non-radiation-hardened trench gate MOSFET with a radiation-hardened planar Si MOSFET, the TID tolerance is improved to over 200 krad(Si), with the V_GS(th) drift controlled within 0.4 V after irradiation and annealing. The oxides in both the cell region and junction termination region of the planar Si MOSFET are specially radiation-hardened by optimizing the device process and structure. Concerning the phenomenon of on resistance (R_DS(on)) increase after irradiation and annealing, the degradation mechanism is investigated through TCAD simulations and analytical models. The reason lies in that during irradiation forming interface states at the AlGaN/GaN interface. The annealing process accelerates the growth of interface states at the Si₃N₄/AlGaN interface. The generation of interface states at these two positions reduces the 2DEG density and mobility in the 2DEG channel, leading to an increase in R_DS(on). Through this study, the relatively mature low-voltage radiation-hardened technology can be applied in the high-voltage field.

传统的级联GaN hemt结合了沟槽栅Si MOSFET和D-HEMT，容易受到总电离剂量（TID）辐射的影响，在相对较低的剂量水平（≤30 krad(Si)）下表现出严重的阈值电压（VGS(th)）退化和功能失效。在本文中，通过集成辐射硬化的Si MOSFET和商用D-HEMT，提出了一种tid硬化的级联GaN HEMT。用平面Si MOSFET代替非辐射硬化沟槽栅极MOSFET，使TID公差提高到200 krad（Si）以上，辐照退火后的VGS（th）漂移控制在0.4 V以内。通过优化器件工艺和结构，对平面硅MOSFET的晶胞区和结端区氧化物进行了特殊的辐射硬化处理。针对辐照退火后电阻（RDS）升高的现象，通过TCAD仿真和解析模型研究了其降解机理。原因是在辐照过程中，在AlGaN/GaN界面处形成界面态。退火过程加速了Si3N4/AlGaN界面态的生长。在这两个位置产生的界面态降低了2DEG通道中的2DEG密度和迁移率，导致RDS（on）增加。通过本研究，可以将较为成熟的低压抗辐射技术应用于高压领域。

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引用次数: 0

Comprehensive identification and experimental validation of programmed cell death-anoikis genes in oral squamous cell carcinoma through machine learning approaches 利用机器学习方法对口腔鳞状细胞癌的程序性细胞死亡基因进行综合鉴定和实验验证

IF 2.5 4区综合性期刊 Q2 MULTIDISCIPLINARY SCIENCES

Journal of Radiation Research and Applied Sciences

Pub Date : 2025-12-22 DOI: 10.1016/j.jrras.2025.102138

Ning Li, Ruoxi Du

Objective

This study aimed to identify anoikis-related genes (ARGs) associated with Oral squamous cell carcinoma (OSCC) progression and prognosis.

Methods

We obtained RNA-sequencing data from TCGA (268 OSCC samples and 19 normal samples) and GEO validation cohort (GSE41613, 97 patients). Candidate anoikis-related genes were identified at the intersection of differentially expressed genes, WGCNA modules, and 338 known ARGs from literature. A comprehensive machine learning framework incorporating 117 algorithm combinations was developed to construct a prognostic risk model, with performance evaluated using concordance index and time-dependent ROC curves. Immune infiltration analysis was performed using the xCell algorithm. Functional validation was conducted through siRNA-mediated knockdown of risk genes and overexpression of protective genes in CAL27 cells, with transfection efficiency confirmed by qRT-PCR and cell viability assessed using CCK-8 assays.

Results

Through integrated bioinformatic analysis of TCGA and GEO datasets, we identified 53 candidate genes at the intersection of differentially expressed genes, weighted gene co-expression network analysis modules, and known ARGs. Functional enrichment analysis revealed significant involvement in biological processes related to anoikis regulation, cell-matrix adhesion, and cancer-related pathways. Using 117 machine learning algorithm combinations, we established a prognostic risk model based on eight ARGs (CTTN, PLAU, ITGA5, MET, TNFRSF12A, PLK1, PDK4, and SESN1), which effectively stratified OSCC patients into high and low-risk groups with significantly different survival outcomes in both training and validation cohorts. Multivariate Cox regression confirmed the risk score as an independent prognostic factor alongside T and N stages. In vitro experiments demonstrated that knockdown of risk genes and overexpression of protective genes significantly reduced cell viability in CAL27 cells.

Conclusion

This study provides novel insights into the molecular mechanisms of anoikis resistance in OSCC and potential therapeutic targets for improving patient outcomes.

目的探讨口腔鳞状细胞癌（OSCC）进展与预后相关的嗜酸相关基因（ARGs）。方法获得TCGA（268例OSCC样本和19例正常样本）和GEO验证队列（GSE41613例，97例患者）的rna测序数据。从文献中的差异表达基因、WGCNA模块和338个已知ARGs的交集处鉴定出候选的嗜酒相关基因。开发了包含117种算法组合的综合机器学习框架来构建预后风险模型，并使用一致性指数和随时间变化的ROC曲线对其性能进行评估。免疫浸润分析采用xCell算法。通过sirna介导的CAL27细胞中风险基因的敲低和保护基因的过表达进行功能验证，用qRT-PCR证实转染效率，用CCK-8测定细胞活力。结果通过TCGA和GEO数据集的综合生物信息学分析，我们在差异表达基因、加权基因共表达网络分析模块和已知ARGs的交集处鉴定出53个候选基因。功能富集分析揭示了与anoikis调节、细胞-基质粘附和癌症相关途径相关的生物过程的显著参与。使用117种机器学习算法组合，我们建立了基于8种ARGs （CTTN、PLAU、ITGA5、MET、TNFRSF12A、PLK1、PDK4和SESN1）的预后风险模型，该模型有效地将OSCC患者分为高危组和低危组，在训练和验证队列中生存结果均有显著差异。多因素Cox回归证实，风险评分与T和N分期一起是独立的预后因素。体外实验表明，风险基因的敲低和保护基因的过表达显著降低了CAL27细胞的活力。结论本研究为OSCC中anoikis耐药的分子机制和改善患者预后的潜在治疗靶点提供了新的见解。

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引用次数: 0

Investigation of gamma-attenuation and optical behavior in La-doped phosphate glasses for electronic shielding materials 电子屏蔽材料中掺la磷酸盐玻璃的γ衰减和光学行为研究

IF 2.5 4区综合性期刊 Q2 MULTIDISCIPLINARY SCIENCES

Journal of Radiation Research and Applied Sciences

Pub Date : 2025-12-20 DOI: 10.1016/j.jrras.2025.102133

Gharam A. Alharshan , Nasra M. Ebrahem , Shaaban M. Shaaban , A.M.A. Mahmoud , R.A. Elsad

Phosphate glasses with a composition of 60 P₂O₅-20 Li₂O-15ZnO- 5Bi₂O₃ and (60-(x+0.5)) P₂O₅-20 Li₂O-15ZnO- 5Bi₂O₃- 0.5 Er₂O₃-xLa₂O₃ with x = 0:1 mol% were formed via a quick melt-quench process in addition to casting. The produced phosphate glass samples exhibit a rise in density as well as molar volume upon substitution of P₂O₅ with a specific ratio of Er₂O₃ and La₂O₃. The UV–visible domain absorption spectra were analyzed to determine optical characteristics of the manufactured glasses. The Davis-Mott method is employed to find the Urbach energy and optical energy band gaps. The refractive index of each created glass sample is also evaluated, and these results are used to calculate the corresponding molar refraction, the metallization criterion and non-linear optical susceptibilities of all samples, using the appropriate formulae. Phy-X/PSD software was utilized to compute the mass attenuation coefficient (MAC) and linear attenuation coefficient (LAC) throughout an energy range of 0.01–15 MeV. Additionally, LAC was detected experimentally at particular energies, and there was good agreement between both experimental and theoretical findings. The accuracy of the findings was confirmed by the same outcomes that both methods produced. Furthermore, transmission factors (TF), radiation protection efficiency (RPE), (half- and tenth-value layers HVL, and TVL were estimated to evaluate the shielding effectiveness of the glass samples. As the quantity of rare earth oxides in mol% increased, radiation shielding items declared a notable enhancement, with an order of S4 > S3 > S2 > S2 > S1 found at all photon energies.

在浇铸的基础上，采用快速熔淬工艺制备了60 P2O5-20 Li2O-15ZnO- 5Bi2O3和（60-(x+0.5)） P2O5-20 Li2O-15ZnO- 5Bi2O3- 0.5 Er2O3-xLa2O3的磷酸盐玻璃，其质量分数为x = 0:1 mol%。用特定比例的Er2O3和La2O3取代P2O5后，制备的磷酸盐玻璃样品的密度和摩尔体积都有所增加。分析了所制玻璃的紫外-可见吸收光谱，确定了其光学特性。采用Davis-Mott方法计算了乌尔巴赫能和光能带隙。对每个玻璃样品的折射率进行了评估，并利用这些结果计算出相应的摩尔折射率、金属化判据和所有样品的非线性光学磁化率。利用Phy-X/PSD软件计算了0.01-15 MeV能量范围内的质量衰减系数（MAC）和线性衰减系数（LAC）。此外，在特定能量下对LAC进行了实验检测，实验结果与理论结果吻合良好。两种方法产生的相同结果证实了结果的准确性。利用透射系数（TF）、辐射防护效率（RPE）、半值层和十值层HVL和TVL来评价玻璃样品的屏蔽效果。随着稀土氧化物摩尔%含量的增加，辐射屏蔽项明显增强，在所有光子能量下均为S4 >； S3 > S2 > S2 > S1。

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引用次数: 0

Concentration dependent gamma degradation of inulin in aqueous phase and its impact on antioxidant capacity and probiotic fermentability 菊粉在水相中浓度依赖性γ降解及其对抗氧化能力和益生菌发酵能力的影响

IF 2.5 4区综合性期刊 Q2 MULTIDISCIPLINARY SCIENCES

Journal of Radiation Research and Applied Sciences

Pub Date : 2025-12-20 DOI: 10.1016/j.jrras.2025.102131

Sudesh , Sahayog N. Jamdar

Inulin is a water soluble, non-digestible polysaccharide with diverse food and clinical applications depending on its degree of polymerization. This study investigated the effect of inulin concentration on its gamma-induced depolymerization in aqueous phase by analysing changes in relative viscosity (η_r), solubility (%), total reducing sugar (TRS), free fructose (F_f) content, and •OH radical scavenging activity. The extent of inulin degradation in aqueous solution was influenced by both irradiation dose and sample concentration. Degradation increased with irradiation dose, while concentration and degree of degradation were in inverse relationship. The level of degradation as a result of molecular fragmentation was confirmed by thin layer chromatography (TLC) and gel permeation chromatography (GPC). FT-IR spectral characterization of unirradiated and irradiated samples revealed no significant changes in peak profiles. Furthermore, concentration-dependent degradation was evaluated for its impact on antioxidant capacity and probiotic fermentability. Inulin irradiated at lower concentrations exhibited significantly higher (p < 0.05) ABTS and DPPH radical scavenging activity and probiotic fermentability than samples irradiated at higher concentrations; specifically, at 25 kGy, inulin irradiated in a 5 % (w/v) aqueous system showed ∼3.5-fold higher antioxidant activity and a ∼2.5-log increase in probiotic fermentability compared to the 60 % (w/v) system.

菊粉是一种水溶性、不可消化的多糖，其聚合程度决定了其在食品和临床中的应用。本研究通过分析菊粉相对粘度（ηr）、溶解度（%）、总还原糖（TRS）、游离果糖（Ff）含量和•OH自由基清除活性的变化，研究了菊粉浓度对其在水相中γ -诱导解聚的影响。菊粉在水溶液中的降解程度受辐照剂量和样品浓度的影响。降解程度随辐照剂量增加而增加，辐照浓度与降解程度呈反比关系。通过薄层色谱（TLC）和凝胶渗透色谱（GPC）证实了分子破碎导致的降解程度。未辐照和辐照样品的FT-IR光谱表征显示峰谱没有显著变化。此外，还评估了浓度依赖性降解对益生菌抗氧化能力和发酵能力的影响。低浓度菊粉对ABTS和DPPH自由基的清除能力和益生菌的发酵能力显著高于高浓度菊粉；具体来说，在25 kGy时，与60% （w/v）的水体系相比，5% （w/v）的菊粉的抗氧化活性提高了约3.5倍，益生菌的发酵能力提高了约2.5倍。

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引用次数: 0

Engineering software-based evaluation of pelvic floor muscle defects in anterior vaginal wall prolapse 基于工程软件的阴道前壁脱垂盆底肌肉缺损的评价

IF 2.5 4区综合性期刊 Q2 MULTIDISCIPLINARY SCIENCES

Journal of Radiation Research and Applied Sciences

Pub Date : 2025-12-20 DOI: 10.1016/j.jrras.2025.102119

Lifan Shen , Huijun Bai

Objective

To assess the relationship between pelvic floor muscle morphology and anterior vaginal wall prolapse using MRI 3D reconstruction and engineering software.

Methods

This study conducted a retrospective analysis involving 68 patients diagnosed with anterior vaginal wall prolapse. Magnetic resonance images (MRI) of the relevant pelvic floor muscles from these patients were utilized for three-dimensional reconstruction using Mimics. Subsequently, the IGS models generated by Geomagic Wrap were employed for material property assignment and analysis within SolidWorks. The muscle models were then transformed into visual simulation entities, facilitating the derivation of metrics such as, volume, and surface area of the muscles associated with anterior vaginal wall prolapse. This methodology allowed for a direct assessment of muscle tissue defects and symmetry.

Results

In patients with anterior vaginal wall prolapse, statistically significant differences (P < 0.05) were observed in the volume, and maximum thickness of the bulbocavernosus muscle relative to the POP-Q grading of anterior vaginal wall prolapse. Whether there was damage to the obturator internus, ischiocavernosus, levator ani muscle, external urethral sphincter, and external anal sphincter muscles was statistically significant in relation to anterior vaginal wall prolapse (P < 0.05). Symmetry of the ischiocavernosus and levator ani muscle also showed statistical significance concerning anterior vaginal wall prolapse (P < 0.05). Factors related to the level of anterior vaginal wall prolapse included the maximum thickness of the bulbocavernosus muscle (OR = 0.005719), damage to the ischiocavernosus muscle (OR = 0.084754), damage to the levator ani muscle (OR = 0.083995), damage to the external urethral sphincter (OR = 0.005917), and damage to the external anal sphincter (OR = 0.004091) (P < 0.05).

Conclusion

Utilizing engineering software and 3D reconstruction to create muscle models offers an impartial and three-dimensional depiction of the morphological alterations in pelvic floor muscles, along with their connection to anterior vaginal wall prolapse. This can help predict and manage the potential impacts on patients.

目的应用MRI三维重建和工程软件研究盆底肌肉形态与阴道前壁脱垂的关系。方法回顾性分析68例阴道前壁脱垂患者的临床资料。这些患者的相关盆底肌肉的磁共振图像（MRI）被利用Mimics进行三维重建。随后，利用Geomagic Wrap生成的IGS模型在SolidWorks中进行材料属性分配和分析。然后将肌肉模型转换为视觉模拟实体，方便推导与阴道前壁脱垂相关的肌肉的体积和表面积等指标。这种方法允许直接评估肌肉组织缺陷和对称性。结果阴道前壁脱垂患者的球海绵体肌体积、最大厚度相对于阴道前壁脱垂的POP-Q分级，差异有统计学意义（P < 0.05）。是否有闭孔内肌、坐骨海威肌、提肛肌、尿道外括约肌、肛门外括约肌受损与阴道前壁脱垂有统计学意义（P < 0.05）。坐骨海绵体和肛提肌的对称性在阴道前壁脱垂方面也有统计学意义（P < 0.05）。影响阴道前壁脱垂程度的因素包括球海绵体肌最大厚度（OR = 0.005719）、坐骨海绵体肌损伤（OR = 0.084754）、提肛肌损伤（OR = 0.083995）、尿道外括约肌损伤（OR = 0.005917）、肛门外括约肌损伤（OR = 0.004091）（P < 0.05）。结论利用工程软件和三维重建技术创建的肌肉模型可以公正、三维地描述骨盆底肌肉的形态学改变，以及它们与阴道前壁脱垂的联系。这有助于预测和管理对患者的潜在影响。

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引用次数: 0

CT and MRI measurement of the femoral anterior tangent for the rotational reference axis of femoral prostheses 股骨假体旋转参考轴的股骨前切线的CT和MRI测量

IF 2.5 4区综合性期刊 Q2 MULTIDISCIPLINARY SCIENCES

Journal of Radiation Research and Applied Sciences

Pub Date : 2025-12-20 DOI: 10.1016/j.jrras.2025.102130

Haibo Liang, Haiquan Deng, Jianchao Sun, Kaitian Wu, Shuzhen Li

Background

This study aims to evaluate the value of the femoral anterior tangent (FAT) as a reference axis for the rotation of femoral prostheses on computed tomography (CT) and magnetic resonance imaging (MRI).

Methods

We retrospectively included 66 patients (83 knees) with primary knee osteoarthritis. FAT, clinical transepicondylar axis (cTEA), surgical transepicondylar axis (sTEA), anterior-posterior axis (APA), perpendicular axis to the posterior-anterior axis of the femoral condyles (pAPA), and posterior condylar axis (PCA) were measured on CT and MRI. The rotation angles of FAT, pAPA, and PCA relative to cTEA and sTEA were measured on the same layer. Patients were further subgrouped based on Kellgren-Lawrence (K-L) grade and gender to assess the variance of different rotational reference axes.

Results

The mean values of FAT/cTEA, FAT/sTEA, and pAPA/cTEA measured on CT were significantly lower, indicating greater internal rotation, than those measured on MRI (P < 0.05). There were no statistical differences between FAT/sTEA, FAT/cTEA, pAPA/cTEA, and PCA/cTEA among genders (P > 0.05). The CT measurement of pAPA/cTEA in K-L grade 3 knees was −1.1° ± 3.7°, significantly higher than in K-L grade 4 knees (P = 0.036). FAT/cTEA, FAT/sTEA, and PCA/cTEA demonstrated higher stability in various subgroups of knee osteoarthritis.

Conclusion

The rotational angles of femoral prostheses measured by CT for FAT and other reference axes show increased internal rotation compared to MRI measurements. Both measurement methods provide relatively stable values for FAT measurements. Clinicians should be cautious of the numerical differences between MRI and CT measurements in clinical applications and should consider modality-specificreference values for pre-operative planning.

本研究旨在评估股骨前切线（FAT）在计算机断层扫描（CT）和磁共振成像（MRI）上作为股骨假体旋转参考轴的价值。方法回顾性分析66例原发性膝骨关节炎患者（83个膝关节）。在CT和MRI上测量FAT、临床经髁突轴（cTEA）、手术经髁突轴（sTEA）、前后轴（APA）、股髁前后轴垂直轴（pAPA）和后髁轴（PCA）。在同一层上测量FAT、pAPA和PCA相对于cTEA和sTEA的旋转角度。进一步根据Kellgren-Lawrence （K-L）分级和性别对患者进行亚组，以评估不同旋转参考轴的方差。结果CT测量的FAT/cTEA、FAT/sTEA、pAPA/cTEA均值较MRI测量值明显降低，内旋较大（P < 0.05）。FAT/sTEA、FAT/cTEA、pAPA/cTEA、PCA/cTEA在性别间差异无统计学意义（P > 0.05）。K-L 3级膝关节的CT测量pAPA/cTEA为- 1.1°±3.7°，显著高于K-L 4级膝关节（P = 0.036）。FAT/cTEA、FAT/sTEA和PCA/cTEA在膝关节骨关节炎的各个亚组中表现出更高的稳定性。结论通过CT测量股骨假体的FAT和其他参考轴的旋转角度，与MRI测量结果相比，内旋增加。两种测量方法都为FAT测量提供了相对稳定的值。临床医生在临床应用中应注意MRI和CT测量值之间的数值差异，并应考虑特定模式的术前计划参考值。

{"title":"CT and MRI measurement of the femoral anterior tangent for the rotational reference axis of femoral prostheses","authors":"Haibo Liang, Haiquan Deng, Jianchao Sun, Kaitian Wu, Shuzhen Li","doi":"10.1016/j.jrras.2025.102130","DOIUrl":"10.1016/j.jrras.2025.102130","url":null,"abstract":"<div><h3>Background</h3><div>This study aims to evaluate the value of the femoral anterior tangent (FAT) as a reference axis for the rotation of femoral prostheses on computed tomography (CT) and magnetic resonance imaging (MRI).</div></div><div><h3>Methods</h3><div>We retrospectively included 66 patients (83 knees) with primary knee osteoarthritis. FAT, clinical transepicondylar axis (cTEA), surgical transepicondylar axis (sTEA), anterior-posterior axis (APA), perpendicular axis to the posterior-anterior axis of the femoral condyles (pAPA), and posterior condylar axis (PCA) were measured on CT and MRI. The rotation angles of FAT, pAPA, and PCA relative to cTEA and sTEA were measured on the same layer. Patients were further subgrouped based on Kellgren-Lawrence (K-L) grade and gender to assess the variance of different rotational reference axes.</div></div><div><h3>Results</h3><div>The mean values of FAT/cTEA, FAT/sTEA, and pAPA/cTEA measured on CT were significantly lower, indicating greater internal rotation, than those measured on MRI (P < 0.05). There were no statistical differences between FAT/sTEA, FAT/cTEA, pAPA/cTEA, and PCA/cTEA among genders (P > 0.05). The CT measurement of pAPA/cTEA in K-L grade 3 knees was −1.1° ± 3.7°, significantly higher than in K-L grade 4 knees (P = 0.036). FAT/cTEA, FAT/sTEA, and PCA/cTEA demonstrated higher stability in various subgroups of knee osteoarthritis.</div></div><div><h3>Conclusion</h3><div>The rotational angles of femoral prostheses measured by CT for FAT and other reference axes show increased internal rotation compared to MRI measurements. Both measurement methods provide relatively stable values for FAT measurements. Clinicians should be cautious of the numerical differences between MRI and CT measurements in clinical applications and should consider modality-specificreference values for pre-operative planning.</div></div>","PeriodicalId":16920,"journal":{"name":"Journal of Radiation Research and Applied Sciences","volume":"19 1","pages":"Article 102130"},"PeriodicalIF":2.5,"publicationDate":"2025-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145837399","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Radiological safety assessment of packaged bottle drinking water in India – Age dependent analysis 印度包装瓶装饮用水的放射性安全评价——年龄相关分析

IF 2.5 4区综合性期刊 Q2 MULTIDISCIPLINARY SCIENCES

Journal of Radiation Research and Applied Sciences

Pub Date : 2025-12-19 DOI: 10.1016/j.jrras.2025.102128

Mahasin Gazi , Arindam Kumar Naskar , Argha Deb

Radioactive radon (²²²Rn) concentrations in 121 different brands of packaged bottle drinking water (PBDW) samples from different states of India have been assessed in the present study using a potable AlphaGUARD radon monitor along with AlphaGUARD aqua kit. Among the collected samples, the production sites of 72 number of PBDW samples are different places of West Bengal and the remaining 49 samples are different places of other different states of India. Thus, assessing the radon levels in different brands of packaged drinking water that are accessed from most parts of India gives a general picture of the ²²²Rn concentrations in packaged bottled drinking water throughout India.

Measured radon concentration levels vary between 0.19 and 5.81 Bq/l with an average value of 1.36 Bq/l. Radon concentration of no sample has exceeded the WHO, IAEA and EU Commission prescribed reference level of 100 Bq/l or even USEPA's proposed MCL (11.11 Bq/l).

The measured radon concentrations of the collected samples manifest no significant correlation with either Total Dissolved Solids (TDS) or pH values of the samples.

Annual effective dose (AED) due to radon from these bottled drinking water for three age groups namely infants, children and adults are found to vary between 0.66 and 20.00 μSv/y with a mean of 4.68 ± 0.29 μSv/y, 0.82–25.00 μSv/y with a mean of 5.84 ± 0.36 μSv/y and 0.73–22.30 μSv/y with a mean of 5.21 ± 0.32 μSv/y respectively. Assessment of dose shows that total annual effective dose to the children is the highest among the age groups. No sample has exceeded the WHO, ICRP and the EU commission proposed reference dose limit of 100 μSv/y.

本研究使用便携式AlphaGUARD氡监测仪和AlphaGUARD aqua试剂盒对来自印度不同邦的121种不同品牌包装瓶装饮用水（PBDW）样品中的放射性氡（222Rn）浓度进行了评估。在收集到的样品中，72个PBDW样品的生产地点位于西孟加拉邦的不同地方，其余49个样品位于印度其他不同邦的不同地方。因此，对从印度大部分地区获取的不同品牌包装饮用水中的氡水平进行评估，可以大致了解印度各地包装瓶装饮用水中222Rn的浓度。测得的氡浓度水平在0.19至5.81 Bq/l之间，平均值为1.36 Bq/l。没有样品的氡浓度超过世界卫生组织、国际原子能机构和欧盟委员会规定的100 Bq/l的参考水平，甚至超过美国环保署提出的MCL （11.11 Bq/l）。所采集样品的氡浓度与样品的总溶解固形物（TDS）或pH值均无显著相关性。对婴幼儿、儿童和成人3个年龄组的氡年有效剂量分别为0.66 ~ 20.00 μSv/y，平均值为4.68±0.29 μSv/y, 0.82 ~ 25.00 μSv/y，平均值为5.84±0.36 μSv/y, 0.73 ~ 22.30 μSv/y，平均值为5.21±0.32 μSv/y。剂量评估表明，儿童的年总有效剂量在各年龄组中最高。没有样品超过世界卫生组织、ICRP和欧盟委员会提出的100 μSv/y的参考剂量限值。

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引用次数: 0