G. Kencana, Tri Komala Sari, I. Suartha, I. K. T. C. Ramanda, A. Kendran
Avian Influenza subtype H5N1 (AI-H5N1) is a malignant virus that is very detrimental to laying chickens because it is highly contagious and mutates easily. Prevention of AI-H5N1 disease in laying chickens is carried out by vaccination, therefore to maintain the quality of the vaccine, continuous research is needed. This study aims to determine the potential of AI-H5N1 vaccine isolates from Bali as measured based on cellular immune response based on total and differential leukocyte cells. Formation of antibodies is influenced by the nonspecific and specific immune system involving leukocytes, especially lymphocytes. Total of 40 layers of Novogen Brown strain were used for the research sample, kept since the age of one day on a commercial farm in Perean Village, Tabanan Regency, Bali. Laying chickens are vaccinated at 5 weeks of age by intramuscular injection. Total of 20 chikens were taken randomly and used for the sample. Blood draws were performed once pre-vaccination and five times each week after vaccination with anticoagulants. Total leukocytes were examined by an auto hematology analyzer, while differential leucocytes with thin blood smear stained with Giemsa. Total and differential leukocyte data were analyzed by means of the variance test followed by the Duncan test. Results showed that AI-H5N1 vaccination from Bali isolates could increase total and differential leucocytes of laying chickens and had significant effect on the mean total leukocytes, the absolute values of heterophyll cells, eosinophils, lymphocytes, and monocytes, but had no significant effect on post-vaccination basophil cells.
{"title":"Respon Imun Seluler Ayam Petelur Pascavaksinasi Avian Influenza Subtipe H5N1 Isolat dari Bali","authors":"G. Kencana, Tri Komala Sari, I. Suartha, I. K. T. C. Ramanda, A. Kendran","doi":"10.22146/jsv.66086","DOIUrl":"https://doi.org/10.22146/jsv.66086","url":null,"abstract":"Avian Influenza subtype H5N1 (AI-H5N1) is a malignant virus that is very detrimental to laying chickens because it is highly contagious and mutates easily. Prevention of AI-H5N1 disease in laying chickens is carried out by vaccination, therefore to maintain the quality of the vaccine, continuous research is needed. This study aims to determine the potential of AI-H5N1 vaccine isolates from Bali as measured based on cellular immune response based on total and differential leukocyte cells. Formation of antibodies is influenced by the nonspecific and specific immune system involving leukocytes, especially lymphocytes. Total of 40 layers of Novogen Brown strain were used for the research sample, kept since the age of one day on a commercial farm in Perean Village, Tabanan Regency, Bali. Laying chickens are vaccinated at 5 weeks of age by intramuscular injection. Total of 20 chikens were taken randomly and used for the sample. Blood draws were performed once pre-vaccination and five times each week after vaccination with anticoagulants. Total leukocytes were examined by an auto hematology analyzer, while differential leucocytes with thin blood smear stained with Giemsa. Total and differential leukocyte data were analyzed by means of the variance test followed by the Duncan test. Results showed that AI-H5N1 vaccination from Bali isolates could increase total and differential leucocytes of laying chickens and had significant effect on the mean total leukocytes, the absolute values of heterophyll cells, eosinophils, lymphocytes, and monocytes, but had no significant effect on post-vaccination basophil cells.","PeriodicalId":17708,"journal":{"name":"Jurnal Sain Veteriner","volume":"40 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79010586","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The mean protein content in broiler chickens from high to low were respectively protein in breast meat from supermarkets: 16.83 ± 0.42 %; breasts from the traditional market: 15.63 ± 1.09 %, thighs from the supermarket: 14.5 ± 0.57 % and thighs from the traditional market: 13.6 ± 0.38 gr / 100 %. Based on all of the datas collection, the result of statistical analysis using a 2x2 factorial pattern showed that there were no significant differences of water content (P>0.05) whreas: the ash, carbohydrates, fats and proteins showed significant (P£0.05).Thighs from traditional markets 4.5 ± 0.60 % and chest from traditional markets from 5.3 ± 0.69 %. The Average of fat content of broiler meat in the thigh, from supermarket: 2.56 ± 0.63 %; chest origin supermarket 1.2 ± 0.5 %; thigh meat from traditional markets: 3.15 ± 0.21 % and breast meat from traditional markets: 1.8 ± 0.227 %. Broiller is one of the biggest contributor to animals protein from livestock and is a superior commodity in Indonesia. At present, the broiler chicken industry has developed rapidly and become the largest contributor to animal protein as well as a major source of consumer menus that are very easy to obtain, both in modern and traditional markets. After the achievement of the population of broiller chickens, government policy began to emphasize on improving the quality of meat by changing meat characteristics such as appearance, texture, moisture content, firmness, softness, odor, taste and the nutritional content is no exception. Thirty-two samples of broiller chicken meat consisting of 16 meat samples purchased from 4 supermarkets and 16 samples purchased from 4 traditional markets were used as research objects. A hundred grams of fresh meat per sample were purchased and immediately packed in aluminum foil, packaged in a cooler box then sent to the Food and Nutrition Laboratory, Inter-University Center, Gadjah Mada University for proximate analysis. The results of the examination of water concentration showed that the average of water content of thigh meat from supermarkets was 76.26 ± 0.86 %; supermarket breast meat 74.135 ± 0.92 %; thigh meat from the traditional market 75 ± 0.56 % and breast meat from the traditional market: 75.64 ± 1.044 %. Analysis of the average levels of the ash concentration respectively: thighs from the supermarket 0.96 ± 0.027 %, chest from the supermarket 1.095 ± 0.05 %, thighs from the traditional market 1.034 ± 0.106 % and breasts from the traditional market 1.155 ± 0.11%. The average of carbohydrate levels were consecutive: Thigh meat from the supermarket: 5.6 ± 1.33 %; chest of origin of the supermarket: 6.7 ± 1.078 %;
{"title":"ANALISIS KUALITAS DAGING AYAM BROILER ASAL PASAR SWALAYAN DAN PASAR TRADISIONAL DI KOTA MEDAN SUMATERA UTARA","authors":"Dhirgo Adji, Angelina Susanty, M. Tafsin","doi":"10.22146/jsv.54354","DOIUrl":"https://doi.org/10.22146/jsv.54354","url":null,"abstract":"The mean protein content in broiler chickens from high to low were respectively protein in breast meat from supermarkets: 16.83 ± 0.42 %; breasts from the traditional market: 15.63 ± 1.09 %, thighs from the supermarket: 14.5 ± 0.57 % and thighs from the traditional market: 13.6 ± 0.38 gr / 100 %. Based on all of the datas collection, the result of statistical analysis using a 2x2 factorial pattern showed that there were no significant differences of water content (P>0.05) whreas: the ash, carbohydrates, fats and proteins showed significant (P£0.05).Thighs from traditional markets 4.5 ± 0.60 % and chest from traditional markets from 5.3 ± 0.69 %. The Average of fat content of broiler meat in the thigh, from supermarket: 2.56 ± 0.63 %; chest origin supermarket 1.2 ± 0.5 %; thigh meat from traditional markets: 3.15 ± 0.21 % and breast meat from traditional markets: 1.8 ± 0.227 %. Broiller is one of the biggest contributor to animals protein from livestock and is a superior commodity in Indonesia. At present, the broiler chicken industry has developed rapidly and become the largest contributor to animal protein as well as a major source of consumer menus that are very easy to obtain, both in modern and traditional markets. After the achievement of the population of broiller chickens, government policy began to emphasize on improving the quality of meat by changing meat characteristics such as appearance, texture, moisture content, firmness, softness, odor, taste and the nutritional content is no exception. Thirty-two samples of broiller chicken meat consisting of 16 meat samples purchased from 4 supermarkets and 16 samples purchased from 4 traditional markets were used as research objects. A hundred grams of fresh meat per sample were purchased and immediately packed in aluminum foil, packaged in a cooler box then sent to the Food and Nutrition Laboratory, Inter-University Center, Gadjah Mada University for proximate analysis. The results of the examination of water concentration showed that the average of water content of thigh meat from supermarkets was 76.26 ± 0.86 %; supermarket breast meat 74.135 ± 0.92 %; thigh meat from the traditional market 75 ± 0.56 % and breast meat from the traditional market: 75.64 ± 1.044 %. Analysis of the average levels of the ash concentration respectively: thighs from the supermarket 0.96 ± 0.027 %, chest from the supermarket 1.095 ± 0.05 %, thighs from the traditional market 1.034 ± 0.106 % and breasts from the traditional market 1.155 ± 0.11%. The average of carbohydrate levels were consecutive: Thigh meat from the supermarket: 5.6 ± 1.33 %; chest of origin of the supermarket: 6.7 ± 1.078 %;","PeriodicalId":17708,"journal":{"name":"Jurnal Sain Veteriner","volume":"11 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80852952","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Abstract Escherichia coli sorbitol-negative in quails cause economic loss due to the death, growth rate inhibition, decreased egg production, and increased medical treatment. Escherichia coli sorbitol-negative has many virulence factors, including hemagglutinin and hemolysin. The aim of this study is to determine the role of hemagglutinin and hemolysin in the infection process of Escherichia coli sorbitol-negative in vitro. This study was performed using 23 isolates of Escherichia coli sorbitol-negative from quails, 52.2% (12 of 23 isolates) had hemagglutinin, while 34.8% (8 out of 23 isolates) had hemolysin. Isolates with hemagglutinin were more attached to human buccal epithelial cells than isolates without hemagglutinin (P <0.05). Isolates with hemolysin were less phagocyted by macrophages compared to isolates which without hemolysin (P <0.05). Escherichia coli sorbitol-negative isolates from quails that have hemagglutinin and hemolysin are pathogenic isolates that possess the potential to cause colibasilosis and transmission between quails and other birds.
{"title":"Peran Hemaglutinin dan Hemolisin pada Escherichia coli Sorbitol-negatif Isolat Burung Puyuh pada Proses Infeksi Secara in Vitro","authors":"Khusnan Khusnan, W. Prihtiyantoro, Dwi Kusmanto","doi":"10.22146/jsv.69896","DOIUrl":"https://doi.org/10.22146/jsv.69896","url":null,"abstract":"Abstract Escherichia coli sorbitol-negative in quails cause economic loss due to the death, growth rate inhibition, decreased egg production, and increased medical treatment. Escherichia coli sorbitol-negative has many virulence factors, including hemagglutinin and hemolysin. The aim of this study is to determine the role of hemagglutinin and hemolysin in the infection process of Escherichia coli sorbitol-negative in vitro. This study was performed using 23 isolates of Escherichia coli sorbitol-negative from quails, 52.2% (12 of 23 isolates) had hemagglutinin, while 34.8% (8 out of 23 isolates) had hemolysin. Isolates with hemagglutinin were more attached to human buccal epithelial cells than isolates without hemagglutinin (P <0.05). Isolates with hemolysin were less phagocyted by macrophages compared to isolates which without hemolysin (P <0.05). Escherichia coli sorbitol-negative isolates from quails that have hemagglutinin and hemolysin are pathogenic isolates that possess the potential to cause colibasilosis and transmission between quails and other birds. ","PeriodicalId":17708,"journal":{"name":"Jurnal Sain Veteriner","volume":"16 7","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91480826","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Deteksi kebuntingan dini pada ternak sapi sangat penting ditinjau dari segi ekonomi karena akan mempengaruhi pendapatan peternak. Deteksi kebuntingan dini sangat penting untuk memperpendek calving interval melalui peningkatan pengetahuan peternak untuk mengidentifikasi status reproduksi, sehingga dapat melakukan terapi dan mengawinkannya sesegera mungkin. Kegiatan penetapan ternak sapi bunting atau tidak bunting dilaksanakan dengan metode deteksi kebuntingan. Penelitian ini bertujuan untuk mengetahui akurasi test strip dalam diagnosis kebuntingan pada ternak sapi. Test strip digunakan untuk mendeteksi kebuntingan 46 ekor ternak sapi Bali betina yang berasal dari peternakan rakyat. Hasil menunjukkan bahwa test strip yang dilakukan pada 30 hari pasca inseminasi buatan menunjukkan sensitivitas 75% dan spesifisitas 90%. Test strip dapat digunakan untuk deteksi kebuntingan dini pada ternak sapi.
{"title":"Deteksi Kebuntingan Ternak Sapi : Aplikasi Test Strip Dairy Cow Pregnancy Colloidal Gold Test Strip","authors":"Sartika Juwita, Mihrani, Agusriady, Aris Handono","doi":"10.22146/jsv.58084","DOIUrl":"https://doi.org/10.22146/jsv.58084","url":null,"abstract":"Deteksi kebuntingan dini pada ternak sapi sangat penting ditinjau dari segi ekonomi karena akan mempengaruhi pendapatan peternak. Deteksi kebuntingan dini sangat penting untuk memperpendek calving interval melalui peningkatan pengetahuan peternak untuk mengidentifikasi status reproduksi, sehingga dapat melakukan terapi dan mengawinkannya sesegera mungkin. Kegiatan penetapan ternak sapi bunting atau tidak bunting dilaksanakan dengan metode deteksi kebuntingan. Penelitian ini bertujuan untuk mengetahui akurasi test strip dalam diagnosis kebuntingan pada ternak sapi. Test strip digunakan untuk mendeteksi kebuntingan 46 ekor ternak sapi Bali betina yang berasal dari peternakan rakyat. Hasil menunjukkan bahwa test strip yang dilakukan pada 30 hari pasca inseminasi buatan menunjukkan sensitivitas 75% dan spesifisitas 90%. Test strip dapat digunakan untuk deteksi kebuntingan dini pada ternak sapi.","PeriodicalId":17708,"journal":{"name":"Jurnal Sain Veteriner","volume":"16 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84691829","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Dewi Pranatasari, Ridon Manik, B. P. Widiarso, W. W. Mubarokah
The problem facing sheep breeders in breeding sheep was digestive tract parasite of worm (nematodiasis and haemonchosis). Resistance to anthelmintics was the reason for the study of alternative medication of H. contortus infection. It aimed at finding out the effectiveness of the application of pineapple peel powder as H. contortus anthelmintic in sheep and the dose of the pineapple peel powder as the H. contortus anthelmintic. It used 15 sheep that were assigned to 5 groups. Group I served as positive control with the application of albendazole (Kalbazen) anthelmintic, Group II was treated using the pineapple peel powder at the dose of 150 mg/kg BW. Group III was treated using the pineapple peel powder at the dose of 200 mg/kg BW. Group IV was treated using the pineapple peel powder at the dose of 250 mg/kg BW. And, Group V served as negative control without any treatment. The treatments were conducted for 14 days and the resulting statistic data were analyzed using comparative descriptive method by comparing initial data (before the treatments) and final data (after the treatments). The comparative data showed that there was significant change in the observed variables. The results of the study showed that the pineapple peel powder could be used as the anthelmintic of the H. contortus in the sheep and the dose of 250 mg/kg BW most significantly decreased the mean number of the eggs of the worm per gram of feces
{"title":"Daya Antelmintik Serbuk Kulit Nanas (Ananas comosus) terhadap Cacing Haemochus contortus pada Domba","authors":"Dewi Pranatasari, Ridon Manik, B. P. Widiarso, W. W. Mubarokah","doi":"10.22146/jsv.66420","DOIUrl":"https://doi.org/10.22146/jsv.66420","url":null,"abstract":"The problem facing sheep breeders in breeding sheep was digestive tract parasite of worm (nematodiasis and haemonchosis). Resistance to anthelmintics was the reason for the study of alternative medication of H. contortus infection. It aimed at finding out the effectiveness of the application of pineapple peel powder as H. contortus anthelmintic in sheep and the dose of the pineapple peel powder as the H. contortus anthelmintic. It used 15 sheep that were assigned to 5 groups. Group I served as positive control with the application of albendazole (Kalbazen) anthelmintic, Group II was treated using the pineapple peel powder at the dose of 150 mg/kg BW. Group III was treated using the pineapple peel powder at the dose of 200 mg/kg BW. Group IV was treated using the pineapple peel powder at the dose of 250 mg/kg BW. And, Group V served as negative control without any treatment. The treatments were conducted for 14 days and the resulting statistic data were analyzed using comparative descriptive method by comparing initial data (before the treatments) and final data (after the treatments). The comparative data showed that there was significant change in the observed variables. The results of the study showed that the pineapple peel powder could be used as the anthelmintic of the H. contortus in the sheep and the dose of 250 mg/kg BW most significantly decreased the mean number of the eggs of the worm per gram of feces","PeriodicalId":17708,"journal":{"name":"Jurnal Sain Veteriner","volume":"46 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85996571","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Abstract Surra's disease is caused by Trypanosoma evansi parasite has been established as one of the strategic infectious animal diseases. Drug resistance in this case is one of the major challenges in handle and control them. The aim of this study is to evaluate the provision drug resistance diminazene aceturate (Tryponil®) on Trypanosoma evansi isolate from Pemalang and Brebes Central Java province with in vivo test in mice. Total 50 mice, BALB / c strain, male, 2 months, body weight ± 30 gram are obtained from LPPT-UGM, adapted for one week. Mice were divided into 10 groups consist of 5 each. Each mouse was infected with Trypanosoma evansi by intraperitonial route. Treatment was given when mice had reached the level of parasitemia 108 – 109 trypanosoma / mL of blood this was predicted 24 hours post-infection (Eisler et al., 2001). The administration of the drug tryapanosidal was done intraperitonial with doses 1mg/kg, 3mg / kg, 5 mg / kg and 7mg / kg. Observation of parasitemia did every 2 times in one week till 60 days of observation. Parasitemia observation was performed using 3 techniques. The first method was native examination used a microscope, if the negative results would be followed by MHCT (Microhaematocrit centrifugation Technique) and BCT (Buffy Coat Technique) according to OIE (2012). Data obtained from the treatment group were the level of parasitemia, the number of deaths and the number of live mice from each test dose. The results are analysed by standard logit or probit. The results of this study showed the effects of the drug Dimianzene aceturate on both isolates varied. On Brebes Isolate was effective at doses of 7 mg / kg BW (100%) and 5mg / kg BW (80%), whereas in the effective dose Pemalang isolate at 3 mg dose / kg BW (80%), 5 and 7 mg / kg BW (100%). While at the lowest dose of 1 mg / kg obtained a level of effectiveness of 0% in both isolates. It could be concluded that both isolates have different pathogens and indicate resistance subpopulation to diminazene aceturate.Keywords : diminazene aceturate, in vivo, resistance, Trypanosoma evansi.
摘要苏拉病是由伊文氏锥虫寄生虫引起的疾病，已被确立为战略传染病之一。在这种情况下，耐药性是处理和控制它们的主要挑战之一。本研究的目的是通过小鼠体内试验，评价中爪哇省培马朗和布里布省埃瓦西锥虫分离株对乙酰氨基苯乙酯(Tryponil®)的耐药性。取BALB / c株雄性，2月龄，体重±30 g, lpt - ugm小鼠50只，适应1周。将小鼠分为10组，每组5只。每只小鼠经腹腔内感染埃氏锥虫。当小鼠达到寄生虫血症水平(108 - 109锥虫/ mL)时给予治疗，这是感染后24小时预测的水平(Eisler等，2001年)。以1mg/kg、3mg /kg、5mg /kg、7mg /kg给药。1周内每2次观察寄生情况，观察60 d。采用3种技术进行寄生虫病观察。第一种方法是使用显微镜进行自然检查，如果结果为阴性，则根据OIE(2012)进行MHCT(微红细胞压层离心技术)和BCT(黄皮技术)。从治疗组获得的数据是每次试验剂量的寄生虫血症水平、死亡数量和活鼠数量。结果用标准logit或probit进行分析。本研究结果表明，乙酸二氨基苯对两种菌株的影响不同。Brebes分离菌的有效剂量为7 mg / kg BW(100%)和5mg / kg BW(80%)，而Pemalang分离菌的有效剂量为3 mg / kg BW(80%)、5和7 mg / kg BW(100%)。而在最低剂量为1mg / kg时，两株菌株的有效性水平均为0%。结果表明，这两种分离株具有不同的致病菌，并存在对醋酸氨基苯乙酯的耐药亚群。关键词:乙酰氨基苯，体内，耐药性，伊氏锥虫
{"title":"Evaluasi Pemberian Obat Diminazene Aceturate Secara In Vivo Pada Mencit (Mus musculus) yang Diinfeksi Isolat Trypanosoma evansi","authors":"Reza Yesica, B. Sutrisno, W. Nurcahyo","doi":"10.22146/JSV.43100","DOIUrl":"https://doi.org/10.22146/JSV.43100","url":null,"abstract":"Abstract Surra's disease is caused by Trypanosoma evansi parasite has been established as one of the strategic infectious animal diseases. Drug resistance in this case is one of the major challenges in handle and control them. The aim of this study is to evaluate the provision drug resistance diminazene aceturate (Tryponil®) on Trypanosoma evansi isolate from Pemalang and Brebes Central Java province with in vivo test in mice. Total 50 mice, BALB / c strain, male, 2 months, body weight ± 30 gram are obtained from LPPT-UGM, adapted for one week. Mice were divided into 10 groups consist of 5 each. Each mouse was infected with Trypanosoma evansi by intraperitonial route. Treatment was given when mice had reached the level of parasitemia 108 – 109 trypanosoma / mL of blood this was predicted 24 hours post-infection (Eisler et al., 2001). The administration of the drug tryapanosidal was done intraperitonial with doses 1mg/kg, 3mg / kg, 5 mg / kg and 7mg / kg. Observation of parasitemia did every 2 times in one week till 60 days of observation. Parasitemia observation was performed using 3 techniques. The first method was native examination used a microscope, if the negative results would be followed by MHCT (Microhaematocrit centrifugation Technique) and BCT (Buffy Coat Technique) according to OIE (2012). Data obtained from the treatment group were the level of parasitemia, the number of deaths and the number of live mice from each test dose. The results are analysed by standard logit or probit. The results of this study showed the effects of the drug Dimianzene aceturate on both isolates varied. On Brebes Isolate was effective at doses of 7 mg / kg BW (100%) and 5mg / kg BW (80%), whereas in the effective dose Pemalang isolate at 3 mg dose / kg BW (80%), 5 and 7 mg / kg BW (100%). While at the lowest dose of 1 mg / kg obtained a level of effectiveness of 0% in both isolates. It could be concluded that both isolates have different pathogens and indicate resistance subpopulation to diminazene aceturate.Keywords : diminazene aceturate, in vivo, resistance, Trypanosoma evansi. ","PeriodicalId":17708,"journal":{"name":"Jurnal Sain Veteriner","volume":"41 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-09-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83827890","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
R. A. Primatika, B. Sumiarto, W. S. Nugroho, Dyah Ayu Widiasih, Y. Drastini, D. Yudhabuntara, H. Susetya
Brucellosis is a Brucella bacterial infectious disease that is spread from animals to humans, generally through consumption of milk, especially unpasteurized milk, or other dairy products. Veterinary public health has an important role in preventing transmission of disease to humans through both animals and foodstuffs of animal origin or other animal origin ingredients. Brucellosis is one of the problems often faced by the Animal Husbandry Department, especially at the Animal Health Unit. Handling and controlling about Brucellosis is very vital to maintain animal health and reduce the level of economic losses of farmers due to Brucellosis experienced by livestock owned. This study aims to determine the spread of Brucellosis disease by mapping in the Administrative and Service Coordination areas, to know the handling and control actions carried out by the Animal Husbandry Service throughout the implementation of the Administrative Coordination Office and Veterinary Public Health. The results of the mapping of the spread of Brucellosis in the District of Veterinary Public Health and Public Service Administration Coordination can be divided into 3 groups with the number of cases <10, 10 - 66, and> 66. The number of cases between the range of 10 - 66 there are 3 regions, while the number of cases> 66 there are 2 regions. Keywords : Brucellosis; Mapping; Handling and Control; Descriptive Statistics
{"title":"Penyebaran Penyakit Brucellosis di Wilayah Koasistensi Administrasi Dinas dan Kesmavet","authors":"R. A. Primatika, B. Sumiarto, W. S. Nugroho, Dyah Ayu Widiasih, Y. Drastini, D. Yudhabuntara, H. Susetya","doi":"10.22146/JSV.51253","DOIUrl":"https://doi.org/10.22146/JSV.51253","url":null,"abstract":"Brucellosis is a Brucella bacterial infectious disease that is spread from animals to humans, generally through consumption of milk, especially unpasteurized milk, or other dairy products. Veterinary public health has an important role in preventing transmission of disease to humans through both animals and foodstuffs of animal origin or other animal origin ingredients. Brucellosis is one of the problems often faced by the Animal Husbandry Department, especially at the Animal Health Unit. Handling and controlling about Brucellosis is very vital to maintain animal health and reduce the level of economic losses of farmers due to Brucellosis experienced by livestock owned. This study aims to determine the spread of Brucellosis disease by mapping in the Administrative and Service Coordination areas, to know the handling and control actions carried out by the Animal Husbandry Service throughout the implementation of the Administrative Coordination Office and Veterinary Public Health. The results of the mapping of the spread of Brucellosis in the District of Veterinary Public Health and Public Service Administration Coordination can be divided into 3 groups with the number of cases <10, 10 - 66, and> 66. The number of cases between the range of 10 - 66 there are 3 regions, while the number of cases> 66 there are 2 regions. Keywords : Brucellosis; Mapping; Handling and Control; Descriptive Statistics","PeriodicalId":17708,"journal":{"name":"Jurnal Sain Veteriner","volume":"219 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-09-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77441232","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Soedarmanto Indarjulianto, Catur Sugiyanto, Ambar Pertiwiningrum, Yanuartono Yanuartono, Alfarisa Nururrozi, Teguh Ari Prabowo, A. Fauzi
Kepincangan pada sapi perah yang dapat diebabkan kuku abnormal dapat mempengaruhi kesehatan dan produksi susu. Penelitian ini bertujuan membandingkan kasus kepincangan akibat kuku abnormal pada sapi perah yang dipelihara di kandang dengan alas karet dan beton. Penelitian ini menggunakan 104 ekor sapi perah dari 23 peternak, yang terdiri dari 72 ekor dipelihara dengan alas kandang karet dan 32 beton. Semua sapi diperiksa kukunya, kemampuan berdiri dan berjalan. Hasil penelitian menunjukkan bahwa prevalensi kasus kuku abnormal sebanyak 26 dari 105 ekor sapi (25%) yang terdiri dari 16/72 ekor (22,2%) pada kandang alas karet dan 10/32 ekor (31,3%) pada kandang alas beton. Kondisi kuku tersebut menyebabkan sebanyak 8 ekor sapi (30,8%) kesulitan berdiri atau kesulitan berjalan dan (69,2%) masih dapat berdiri dan berjalan dengan normal. Sebanyak 6/16 ekor (37,5%) sapi pada kandang alas karet dan 2/10 ekor (20%) sapi pada kandang alas beton menunjukkan kesulitan berdiri dan berjalan. Abnormalitas kuku pada penelitian ini kemungkinan disebabkan kuku tidak dipotong tepat waktu karena peternak kurang berpengalaman. Kesimpulan dari peneltian ini adalah prevalensi problem kuku abnormal adalah 25% yang didapatkan lebih banyak terjadi pada kandang alas beton. Kepincangan akibat kuku abnormal terjadi pada 30,8% sapi perah kuku abnormal dan kejadian didapatkan lebih banyak pada sapi yang dipelihara di kandang dengan alas karet.
{"title":"Kepincangan Akibat Kuku Abnormal Sapi Perah di Kandang dengan Alas Karet dan Beton","authors":"Soedarmanto Indarjulianto, Catur Sugiyanto, Ambar Pertiwiningrum, Yanuartono Yanuartono, Alfarisa Nururrozi, Teguh Ari Prabowo, A. Fauzi","doi":"10.22146/JSV.61684","DOIUrl":"https://doi.org/10.22146/JSV.61684","url":null,"abstract":"Kepincangan pada sapi perah yang dapat diebabkan kuku abnormal dapat mempengaruhi kesehatan dan produksi susu. Penelitian ini bertujuan membandingkan kasus kepincangan akibat kuku abnormal pada sapi perah yang dipelihara di kandang dengan alas karet dan beton. Penelitian ini menggunakan 104 ekor sapi perah dari 23 peternak, yang terdiri dari 72 ekor dipelihara dengan alas kandang karet dan 32 beton. Semua sapi diperiksa kukunya, kemampuan berdiri dan berjalan. Hasil penelitian menunjukkan bahwa prevalensi kasus kuku abnormal sebanyak 26 dari 105 ekor sapi (25%) yang terdiri dari 16/72 ekor (22,2%) pada kandang alas karet dan 10/32 ekor (31,3%) pada kandang alas beton. Kondisi kuku tersebut menyebabkan sebanyak 8 ekor sapi (30,8%) kesulitan berdiri atau kesulitan berjalan dan (69,2%) masih dapat berdiri dan berjalan dengan normal. Sebanyak 6/16 ekor (37,5%) sapi pada kandang alas karet dan 2/10 ekor (20%) sapi pada kandang alas beton menunjukkan kesulitan berdiri dan berjalan. Abnormalitas kuku pada penelitian ini kemungkinan disebabkan kuku tidak dipotong tepat waktu karena peternak kurang berpengalaman. Kesimpulan dari peneltian ini adalah prevalensi problem kuku abnormal adalah 25% yang didapatkan lebih banyak terjadi pada kandang alas beton. Kepincangan akibat kuku abnormal terjadi pada 30,8% sapi perah kuku abnormal dan kejadian didapatkan lebih banyak pada sapi yang dipelihara di kandang dengan alas karet.","PeriodicalId":17708,"journal":{"name":"Jurnal Sain Veteriner","volume":"2 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-09-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87635165","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Kebar grass contains active compounds that can be used as herbal ingredients in the treatment of diseases. This study was conducted to test the anthelmintic activity of grass kebar against worms Ascaridia galli in vitro. This study uses Kebar grass juice and infusion with a concentration of 15%, 30%, 45%, and 60%, and 4 repetitions. Each level of the experiment is placed in each cup containing 25 ml of solution and 5 worms. Worm mortality is recorded every 2 hours. The results showed that the juice and infusion of kebar grass were concentrations of 15%, 30%, 45%, 60% capable of killing worms with a mean time on the juice of Kebar grass respectively 9.5; 8; 7.5; 7 hours, and the average time for Kebar grass infusion is 9.5; 8.5; 8; 7.5 hours. The immersion time is a good variable to explain the variable of worm death at each concentration of treatment. There is an anthelmintic effect on grass juice and infuse kebar grass against worms Ascaridia galli in vitro. The duration of soaking and the concentration of juice and infusion of Kebar grass in this study had a significant effect on the mortality of worms. It was concluded that the juice and grass infuse kebar(Biophytum Petersianum Klotzsch) have anthelmintic effect against worms Ascaridia galli in vitro. Concentration Kebar grass juice and infuse kebar is increasing, then the shorter the time it takes to kill the worms Ascaridia galli in vitro.
{"title":"Test the Activity of the Juice and Infusion of Rumput Kebar (Biophytum petersianum Klotzsch) againts Ascaridia galli worms in Vitro","authors":"I. Widayati, D. Nurhayati, A. Baaka","doi":"10.22146/JSV.48550","DOIUrl":"https://doi.org/10.22146/JSV.48550","url":null,"abstract":"Kebar grass contains active compounds that can be used as herbal ingredients in the treatment of diseases. This study was conducted to test the anthelmintic activity of grass kebar against worms Ascaridia galli in vitro. This study uses Kebar grass juice and infusion with a concentration of 15%, 30%, 45%, and 60%, and 4 repetitions. Each level of the experiment is placed in each cup containing 25 ml of solution and 5 worms. Worm mortality is recorded every 2 hours. The results showed that the juice and infusion of kebar grass were concentrations of 15%, 30%, 45%, 60% capable of killing worms with a mean time on the juice of Kebar grass respectively 9.5; 8; 7.5; 7 hours, and the average time for Kebar grass infusion is 9.5; 8.5; 8; 7.5 hours. The immersion time is a good variable to explain the variable of worm death at each concentration of treatment. There is an anthelmintic effect on grass juice and infuse kebar grass against worms Ascaridia galli in vitro. The duration of soaking and the concentration of juice and infusion of Kebar grass in this study had a significant effect on the mortality of worms. It was concluded that the juice and grass infuse kebar(Biophytum Petersianum Klotzsch) have anthelmintic effect against worms Ascaridia galli in vitro. Concentration Kebar grass juice and infuse kebar is increasing, then the shorter the time it takes to kill the worms Ascaridia galli in vitro.","PeriodicalId":17708,"journal":{"name":"Jurnal Sain Veteriner","volume":"99 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-09-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74474063","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Calcitriol supplementation in ovariectomized rats for 8 weeks reduce osteoporosis risk and safe for kidney depend a proper dose. The objective of the research was to study the efectivity of high dose calcitriol for osteoporosis prevention and safe for kidney in ovariectomized Wistar rats. Fifteen female Wistar rats at 8 weeks of age were randomly divided into three groups of five which were sham operated rats (KN), ovariectomized rats (KOV), and ovariectomized rats+calcitriol supplementation 200ng/day (OVD200). After 8 weeks of treatment, blood samples were taken from plexus orbitalis medialis for estrogen analysis. All rats were then euthanized, left tibia and left kidney were taken for histopathological examination using hematoxylin and eosin stain. Immunohistochemistry using anti TRAP5b monoclonal antibody was also done for left tibia by streptavidin-biotin. The results showed that estradiol level of KOV was significantly decreased compared with KN (P<0.05), meanwhile, estradiol levels of OVD200 were not significantly different compared with KOV. Histopathologic figure of kidney in KOV was not different compared with KN. Glomerulus was surrounded by Bowman‘s capsule, proximal and distal convoluted tubulus were lined with typical cuboidal epithelium. Immunohistochemistry result of proximal tibial epiphysis in KOV were shown more tartrate resistant alkaline phosphatase 5b (TRAP5b) expression in trabecular bone, which was located in bone marrow space, and trabecular speculum surface as well. Meanwhile, proximal tibia epiphysis in OVD200 had less tartrate resistant alkaline phosphatase 5b (TRAP5b) expression compared with KOV. Histopathologic figure of proximal tibia epiphysis in group OVD200 was shown more trabecular bone speculum and less adipocyte in the bone marrow compared with KOV. Histopathologic figure of kidney in OVD200 was showed glomerular hypertrophy and atrophy, as well as protein deposits in proximal convoluted tubules epithelium. In conclusion, calcitriol supplementation 200ng/day in ovariectomized rats for 8 weeks prevented osteoporosis risk but trigger urolithiasis. Key words : : Calcitriol, osteoporosis, ovariectomized rats, urolithiasis
{"title":"Suplemen Calcitriol Dosis Tinggi Mencegah Osteoporosis dan Memicu Urolitiasis pada Tikus Ovariektomi","authors":"Hartiningsih Hartiningsih, Devita Anggraeni","doi":"10.22146/JSV.47032","DOIUrl":"https://doi.org/10.22146/JSV.47032","url":null,"abstract":"Calcitriol supplementation in ovariectomized rats for 8 weeks reduce osteoporosis risk and safe for kidney depend a proper dose. The objective of the research was to study the efectivity of high dose calcitriol for osteoporosis prevention and safe for kidney in ovariectomized Wistar rats. Fifteen female Wistar rats at 8 weeks of age were randomly divided into three groups of five which were sham operated rats (KN), ovariectomized rats (KOV), and ovariectomized rats+calcitriol supplementation 200ng/day (OVD200). After 8 weeks of treatment, blood samples were taken from plexus orbitalis medialis for estrogen analysis. All rats were then euthanized, left tibia and left kidney were taken for histopathological examination using hematoxylin and eosin stain. Immunohistochemistry using anti TRAP5b monoclonal antibody was also done for left tibia by streptavidin-biotin. The results showed that estradiol level of KOV was significantly decreased compared with KN (P<0.05), meanwhile, estradiol levels of OVD200 were not significantly different compared with KOV. Histopathologic figure of kidney in KOV was not different compared with KN. Glomerulus was surrounded by Bowman‘s capsule, proximal and distal convoluted tubulus were lined with typical cuboidal epithelium. Immunohistochemistry result of proximal tibial epiphysis in KOV were shown more tartrate resistant alkaline phosphatase 5b (TRAP5b) expression in trabecular bone, which was located in bone marrow space, and trabecular speculum surface as well. Meanwhile, proximal tibia epiphysis in OVD200 had less tartrate resistant alkaline phosphatase 5b (TRAP5b) expression compared with KOV. Histopathologic figure of proximal tibia epiphysis in group OVD200 was shown more trabecular bone speculum and less adipocyte in the bone marrow compared with KOV. Histopathologic figure of kidney in OVD200 was showed glomerular hypertrophy and atrophy, as well as protein deposits in proximal convoluted tubules epithelium. In conclusion, calcitriol supplementation 200ng/day in ovariectomized rats for 8 weeks prevented osteoporosis risk but trigger urolithiasis. Key words : : Calcitriol, osteoporosis, ovariectomized rats, urolithiasis","PeriodicalId":17708,"journal":{"name":"Jurnal Sain Veteriner","volume":"156 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-09-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76092979","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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