Pub Date : 2021-01-28DOI: 10.53864/jifakfar.v2i1.16
Hadi Barru Hakam Fajar Siddiq, Jati Riyuwani, Rizka Della Yunita Dewi
The purpose of this research is determine the polyphenol content of various brand of packaged tea. Sample were used five sample. Polyphenol compounds of packaged tea were extracted using remaseration method with ethanol 70%. Qualitative test were used using FeCl3 reagent and quantitative test polyphenol content were determined using UV-Vis spectrophotometry method with Folin Ciocalteau reagent. Galic acid was used as comparator in this research. The result of qualitative test is all sample of brewed tea containing polyphenol. Polyphenol content sample A, B, C, D, and E with remaseration method were respectively 0,541; 0,557; 0,474; 0,557; and 0,428%(b/b).Keyword: packaged tea, polyphenol, remaseration, UV-Vis spectrophotometryThe purpose of this research is determine the polyphenol content of various brand of packaged tea. Sample were used five sample. Polyphenol compounds of packaged tea were extracted using remaseration method with ethanol 70%. Qualitative test were used using FeCl3 reagent and quantitative test polyphenol content were determined using UV-Vis spectrophotometry method with Folin Ciocalteau reagent. Galic acid was used as comparator in this research. The result of qualitative test is all sample of brewed tea containing polyphenol. Polyphenol content sample A, B, C, D, and E with remaseration method were respectively 0,541; 0,557; 0,474; 0,557; and 0,428%(b/b).Keyword: packaged tea, polyphenol, remaseration, UV-Vis spectrophotometryPENDAHULUANTeh merupakan minuman yang dapat diterima oleh
{"title":"PENENTUAN KADAR POLIFENOL EKSTRAK TEH KEMASAN DENGAN METODE REMASERASI MENGGUNAKAN SPEKTROFOTOMETRI UV-VIS","authors":"Hadi Barru Hakam Fajar Siddiq, Jati Riyuwani, Rizka Della Yunita Dewi","doi":"10.53864/jifakfar.v2i1.16","DOIUrl":"https://doi.org/10.53864/jifakfar.v2i1.16","url":null,"abstract":"The purpose of this research is determine the polyphenol content of various brand of packaged tea. Sample were used five sample. Polyphenol compounds of packaged tea were extracted using remaseration method with ethanol 70%. Qualitative test were used using FeCl3 reagent and quantitative test polyphenol content were determined using UV-Vis spectrophotometry method with Folin Ciocalteau reagent. Galic acid was used as comparator in this research. The result of qualitative test is all sample of brewed tea containing polyphenol. Polyphenol content sample A, B, C, D, and E with remaseration method were respectively 0,541; 0,557; 0,474; 0,557; and 0,428%(b/b).Keyword: packaged tea, polyphenol, remaseration, UV-Vis spectrophotometryThe purpose of this research is determine the polyphenol content of various brand of packaged tea. Sample were used five sample. Polyphenol compounds of packaged tea were extracted using remaseration method with ethanol 70%. Qualitative test were used using FeCl3 reagent and quantitative test polyphenol content were determined using UV-Vis spectrophotometry method with Folin Ciocalteau reagent. Galic acid was used as comparator in this research. The result of qualitative test is all sample of brewed tea containing polyphenol. Polyphenol content sample A, B, C, D, and E with remaseration method were respectively 0,541; 0,557; 0,474; 0,557; and 0,428%(b/b).Keyword: packaged tea, polyphenol, remaseration, UV-Vis spectrophotometryPENDAHULUANTeh merupakan minuman yang dapat diterima oleh","PeriodicalId":17737,"journal":{"name":"JURNAL ILMIAH FARMASI AKADEMI FARMASI JEMBER","volume":"42 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-01-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90398681","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-01-28DOI: 10.53864/jifakfar.v3i1.37
Rosida, Kukuh Judy Handojo
Tuberculosis is an infectious disease caused by Mycobacterium tuberculosis. Tuberculosis can infect anyone in the patient's environment. Someone with good body immunity conditions will avoid tuberculosis. Increased immunity for tuberculosis sufferers is very important. The active substances contained in plants such as flavonoids and essential oils are important components in supporting the body's immunity. One plant that contains flavonoids and essential oils is kencur (Kaemferia galanga L.). This study aims to prove the potential of kencur extract as an immunomodulator in model rat infected with Mycobacterium tuberculosis. This study was a laboratory experiment using the kencur extract (EK) and samples of rats as a model. The sample used was 30 animals which were divided into 6 groups. Group 1 was a healthy animal (Normal). Groups 2, 3, 4 and 5 were treatment groups consisting of sick animals given placebo treatment (control), rifampicin (R), EK (Kencur Extract) and CRK (Mixture of Rifampicin and Kencur). Group 6 was a normal group given EK then infected with Mycobacterium tuberculosis (NK). Modeling was done by infecting experimental animals using Mycobacterium tuberculosis. The infection was 30 days and the treatment was 21 days. The parameters in this study were leukocytes and Laju Endap Darah (LED). The results of the kencur extract phytochemical screening study showed the presence of flavonoids. The results of the treatment showed that differences in the number of Leukocytes and LEDs between the control group and the treatment (sig 0.05). These results indicate that kencur extract has the potential as an immunomodulator.Keywords : Kaemferia galanga, Mycobacterium tuberculosis, imunomodulator
结核病是一种由结核分枝杆菌引起的传染病。结核病可以感染病人周围的任何人。身体免疫力好的人会避免患肺结核。提高结核病患者的免疫力非常重要。植物中含有的活性物质，如类黄酮和精油，是支持人体免疫力的重要成分。一种含有黄酮类化合物和精油的植物是kencur (Kaemferia galanga L.)。本研究旨在证明kencur提取物对结核分枝杆菌感染模型大鼠的免疫调节剂作用。本研究是以kencur提取物(EK)和大鼠标本为模型的室内实验。实验选用30只动物，分为6组。1组为健康动物(正常)。第2、3、4和5组为治疗组，由给予安慰剂(对照)、利福平(R)、Kencur提取物EK和CRK(利福平和Kencur混合物)的病鼠组成。第6组为正常组，给予EK后感染结核分枝杆菌(NK)。用结核分枝杆菌感染实验动物建立模型。感染30天，治疗21天。本研究的参数为白细胞和Laju Endap Darah (LED)。对莪术提取物进行了植物化学筛选，结果表明莪术提取物中含有黄酮类化合物。治疗结果显示，对照组与治疗组白细胞和发光二极管数量差异有统计学意义(p < 0.05)。这些结果表明，kencur提取物具有作为免疫调节剂的潜力。关键词:高良姜，结核分枝杆菌，免疫调节剂
{"title":"POTENSI EKSTRAK KENCUR (Kaemferia galanga L.) SEBAGAI IMUNOMODULATOR PADA TIKUS MODEL YANG TERINFEKSI Mycobacterium tuberculosis","authors":"Rosida, Kukuh Judy Handojo","doi":"10.53864/jifakfar.v3i1.37","DOIUrl":"https://doi.org/10.53864/jifakfar.v3i1.37","url":null,"abstract":"Tuberculosis is an infectious disease caused by Mycobacterium tuberculosis. Tuberculosis can infect anyone in the patient's environment. Someone with good body immunity conditions will avoid tuberculosis. Increased immunity for tuberculosis sufferers is very important. The active substances contained in plants such as flavonoids and essential oils are important components in supporting the body's immunity. One plant that contains flavonoids and essential oils is kencur (Kaemferia galanga L.). This study aims to prove the potential of kencur extract as an immunomodulator in model rat infected with Mycobacterium tuberculosis. This study was a laboratory experiment using the kencur extract (EK) and samples of rats as a model. The sample used was 30 animals which were divided into 6 groups. Group 1 was a healthy animal (Normal). Groups 2, 3, 4 and 5 were treatment groups consisting of sick animals given placebo treatment (control), rifampicin (R), EK (Kencur Extract) and CRK (Mixture of Rifampicin and Kencur). Group 6 was a normal group given EK then infected with Mycobacterium tuberculosis (NK). Modeling was done by infecting experimental animals using Mycobacterium tuberculosis. The infection was 30 days and the treatment was 21 days. The parameters in this study were leukocytes and Laju Endap Darah (LED). The results of the kencur extract phytochemical screening study showed the presence of flavonoids. The results of the treatment showed that differences in the number of Leukocytes and LEDs between the control group and the treatment (sig 0.05). These results indicate that kencur extract has the potential as an immunomodulator.Keywords : Kaemferia galanga, Mycobacterium tuberculosis, imunomodulator","PeriodicalId":17737,"journal":{"name":"JURNAL ILMIAH FARMASI AKADEMI FARMASI JEMBER","volume":"40 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-01-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73923437","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-01-28DOI: 10.53864/jifakfar.v2i2.22
Acivrida Mega Charisma, Farida Anwari
Cervical cancer is the number one cause of death in Indonesian women. Cervical cancer is a malignant tumor that grows inside the cervix / cervix is the lowest part of the uterus attached to the top of the vagina.Terjantung cancer always begins with precancerous conditions which further leads to advanced cancer conditions. Pap smear test is a test that is accurate enough to detect early cervical cancer if routinely performed, because this test can detect any abnormal cells changes. This study aims to determine the relationship between sexual activity performed at an early age by the respondents with the results of pap smear examination.This research use cross sectional analysis method, simple random sampling sample of 184 people. The result is made of frequency table, cross tabulation and then analyzed by chi square test and spearman correlation test.The results showed that the results of Pap smears class II and III (Not In Normal Limit) on the respondents who do sexual activity at age 18 years there are 45 (71.4%) people and the respondents who do not do sexual activity at age 18 years only 41 (33.9%) people. The same thing happened for the age factor, in respondents with age 35 (at risk) there were 66 (54,2%) people who had the result of examination of Class II and III papsmear (Not in Normal Limit) and at age 35 years old only 20 (33.3%) persons who have the results of papsmear examinations Class II and II (Not In Normal Limit). The analysis using chi square test showed significant correlation between age and early age sexual activity with pap smear with indigo ρ = 0.025 and ρ = 0.018 (α = 0.05).The hope of this study can be an important lesson for the younger generation to be able to control the association in order to avoid doing activities at the age of too young and for all married women (ever doing sexual activity) to perform pap smear examination early to detect any abnormalities before developed into cancer.Keywords : early childhood sexual activity, cervical cancer, pap smear, cervical cancer risk factor
{"title":"HUBUNGAN AKTIVITAS SEKSUAL USIA DINI DENGAN HASIL PEMERIKSAAN PAP SMEAR SEBAGAI DETEKSI DINI KANKER SERVIKS DI KABUPATEN SIDOARJO","authors":"Acivrida Mega Charisma, Farida Anwari","doi":"10.53864/jifakfar.v2i2.22","DOIUrl":"https://doi.org/10.53864/jifakfar.v2i2.22","url":null,"abstract":"Cervical cancer is the number one cause of death in Indonesian women. Cervical cancer is a malignant tumor that grows inside the cervix / cervix is the lowest part of the uterus attached to the top of the vagina.Terjantung cancer always begins with precancerous conditions which further leads to advanced cancer conditions. Pap smear test is a test that is accurate enough to detect early cervical cancer if routinely performed, because this test can detect any abnormal cells changes. This study aims to determine the relationship between sexual activity performed at an early age by the respondents with the results of pap smear examination.This research use cross sectional analysis method, simple random sampling sample of 184 people. The result is made of frequency table, cross tabulation and then analyzed by chi square test and spearman correlation test.The results showed that the results of Pap smears class II and III (Not In Normal Limit) on the respondents who do sexual activity at age 18 years there are 45 (71.4%) people and the respondents who do not do sexual activity at age 18 years only 41 (33.9%) people. The same thing happened for the age factor, in respondents with age 35 (at risk) there were 66 (54,2%) people who had the result of examination of Class II and III papsmear (Not in Normal Limit) and at age 35 years old only 20 (33.3%) persons who have the results of papsmear examinations Class II and II (Not In Normal Limit). The analysis using chi square test showed significant correlation between age and early age sexual activity with pap smear with indigo ρ = 0.025 and ρ = 0.018 (α = 0.05).The hope of this study can be an important lesson for the younger generation to be able to control the association in order to avoid doing activities at the age of too young and for all married women (ever doing sexual activity) to perform pap smear examination early to detect any abnormalities before developed into cancer.Keywords : early childhood sexual activity, cervical cancer, pap smear, cervical cancer risk factor","PeriodicalId":17737,"journal":{"name":"JURNAL ILMIAH FARMASI AKADEMI FARMASI JEMBER","volume":"21 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-01-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79950748","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-01-28DOI: 10.53864/jifakfar.v3i1.36
Mikhania C.E., Agnis Pondinekaria Aditama, Sri Agita Ningrum
The aim of this research was to know the influence of HPMC variation concentration as gelling agent againts physical properties antifungal extract galangal gel (Alpinia galanga (L) Swartz). Pre-experimental the one shot case study was used as the research design. Gel is formulated into three formulas with various concentration of HPMC 2% (F1), 3% (F2), and 4% (F3). The materials used in this research were galangal extract, propylenglycol, glycerin, methyl paraben, and aquadest. The result showed that the variation concentration of HPMC give influence to physical properties of organoleptic gel (form, smell and colour), viscosity, and spreadibility of gel but did not influence homogeneity and pH of the gel.Keywords: gel, galangal extract, HPMC
{"title":"PENGARUH VARIASI KONSENTRASI HPMC SEBAGAI GELLING AGENT TERHADAP SIFAT FISIK GEL ANTIJAMUR EKSTRAK LENGKUAS (Alpinia galanga (L.) Swartz)","authors":"Mikhania C.E., Agnis Pondinekaria Aditama, Sri Agita Ningrum","doi":"10.53864/jifakfar.v3i1.36","DOIUrl":"https://doi.org/10.53864/jifakfar.v3i1.36","url":null,"abstract":"The aim of this research was to know the influence of HPMC variation concentration as gelling agent againts physical properties antifungal extract galangal gel (Alpinia galanga (L) Swartz). Pre-experimental the one shot case study was used as the research design. Gel is formulated into three formulas with various concentration of HPMC 2% (F1), 3% (F2), and 4% (F3). The materials used in this research were galangal extract, propylenglycol, glycerin, methyl paraben, and aquadest. The result showed that the variation concentration of HPMC give influence to physical properties of organoleptic gel (form, smell and colour), viscosity, and spreadibility of gel but did not influence homogeneity and pH of the gel.Keywords: gel, galangal extract, HPMC","PeriodicalId":17737,"journal":{"name":"JURNAL ILMIAH FARMASI AKADEMI FARMASI JEMBER","volume":"53 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-01-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82698504","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-01-28DOI: 10.53864/jifakfar.v2i1.145
Siti Nur Azizah
Kandungan selulosa pada jerami padi tergolong tinggi yaitu sekitar 35%. Sebagai senyawa yang paling melimpah di dunia, selulosa dari jerami padi dapat digunakan sebagai sumber penghasil bakteri selulolitik yang dapat menghasilkan enzim selulase sebagai katalis yang bermanfaat dalam bidang industri, pertanian dan kesehatan. Tujuan dari penelitian ini adalah untuk mendapatkan isolat bakteri selulolitik asal jerami padi di Desa Kampung Jawa Kelurahan Setigede Kecamatan Bogor Barat dengan cara isolasi dan karakterisasi isolat yang didapat pada medium CMC agar. Hasil isolasi pada media CMC agar diperoleh sebanyak 5 isolat bakteri selulolitik yaitu isolat J1, J2, J3,J4 dan J5. Hasil skring aktivitas selulolik secara semikuantitatif menggunakan metode congo red menunjukkan bahwa isolat J2 memiliki nilai IAE tertinggi yaitu 1,84. Hasil karakterisasi secara fisik yaitu makroskopis dan mikroskopis serta karakterisasi secara biokimia sesuai petunjuk Bergey’s Manual of systematic bacteriology menunjukkan bahwa isolat J2 memiliki kemiripan dengan Bacillus sp.
{"title":"ISOLASI DAN KARAKTERISASI BAKTERI SELULOLITIK ASAL JERAMI PADI DI PERSAWAHAN BOGOR BARAT","authors":"Siti Nur Azizah","doi":"10.53864/jifakfar.v2i1.145","DOIUrl":"https://doi.org/10.53864/jifakfar.v2i1.145","url":null,"abstract":"Kandungan selulosa pada jerami padi tergolong tinggi yaitu sekitar 35%. Sebagai senyawa yang paling melimpah di dunia, selulosa dari jerami padi dapat digunakan sebagai sumber penghasil bakteri selulolitik yang dapat menghasilkan enzim selulase sebagai katalis yang bermanfaat dalam bidang industri, pertanian dan kesehatan. Tujuan dari penelitian ini adalah untuk mendapatkan isolat bakteri selulolitik asal jerami padi di Desa Kampung Jawa Kelurahan Setigede Kecamatan Bogor Barat dengan cara isolasi dan karakterisasi isolat yang didapat pada medium CMC agar. Hasil isolasi pada media CMC agar diperoleh sebanyak 5 isolat bakteri selulolitik yaitu isolat J1, J2, J3,J4 dan J5. Hasil skring aktivitas selulolik secara semikuantitatif menggunakan metode congo red menunjukkan bahwa isolat J2 memiliki nilai IAE tertinggi yaitu 1,84. Hasil karakterisasi secara fisik yaitu makroskopis dan mikroskopis serta karakterisasi secara biokimia sesuai petunjuk Bergey’s Manual of systematic bacteriology menunjukkan bahwa isolat J2 memiliki kemiripan dengan Bacillus sp.","PeriodicalId":17737,"journal":{"name":"JURNAL ILMIAH FARMASI AKADEMI FARMASI JEMBER","volume":"31 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-01-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89288927","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-01-28DOI: 10.53864/jifakfar.v1i2.10
Hadi Barru Hakam Fajar Siddiq, Rizka Del Rizka Della Yunita Dewi, Jati Riyuwani
The purpose of this research is determine the polyphenol content of brewed tea. Sample were used three sample. Polyphenol compounds of packaged tea were extracted using percolation method with ethanol 70%. Qualitative test were used using FeCl3 reagent and quantitative test polyphenol content were determined using UV-Vis spectrophotometry method with Folin Ciocalteau reagent. Galic acid was used as comparator in this research. The result of qualitative test is all sample of packaged tea containing polyphenol. Polyphenol content sample A, B, and C, with peercolation method were respectively 0,538; 0,554; and 0,470; %b/b.Keyword: packaged tea, polyphenol, percolation, UV-Vis spectrophotometry
本研究的目的是测定泡茶中多酚的含量。样本分别采用三个样本。采用70%乙醇浸出法提取包装茶中的多酚类化合物。定性试验采用FeCl3试剂，定量试验采用福林试剂紫外-可见分光光度法测定多酚含量。本研究以没食子酸为比较剂。定性检测结果均为含多酚包装茶样品。样品A、B、C的多酚含量分别为0.538;0554;和0470年;% b / b。关键词:包装茶，多酚，渗透，紫外可见分光光度法
{"title":"ANALISIS POLIFENOL EKSTRAK TEH TUBRUK KEMASAN MENGGUNAKAN SPEKTROFOTOMETRI UV-VIS","authors":"Hadi Barru Hakam Fajar Siddiq, Rizka Del Rizka Della Yunita Dewi, Jati Riyuwani","doi":"10.53864/jifakfar.v1i2.10","DOIUrl":"https://doi.org/10.53864/jifakfar.v1i2.10","url":null,"abstract":"The purpose of this research is determine the polyphenol content of brewed tea. Sample were used three sample. Polyphenol compounds of packaged tea were extracted using percolation method with ethanol 70%. Qualitative test were used using FeCl3 reagent and quantitative test polyphenol content were determined using UV-Vis spectrophotometry method with Folin Ciocalteau reagent. Galic acid was used as comparator in this research. The result of qualitative test is all sample of packaged tea containing polyphenol. Polyphenol content sample A, B, and C, with peercolation method were respectively 0,538; 0,554; and 0,470; %b/b.Keyword: packaged tea, polyphenol, percolation, UV-Vis spectrophotometry","PeriodicalId":17737,"journal":{"name":"JURNAL ILMIAH FARMASI AKADEMI FARMASI JEMBER","volume":"12 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-01-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90071727","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-01-28DOI: 10.53864/jifakfar.v1i2.14
Siti Nur Azizah, Lutfiya Cahyani, Reni Budiarti, Rudju Winarsa
Blotong mushroom is a fungus that thrives in waste of filter cake (a solid waste of sugar mill) from PG Semboro, Jember Regency.Commonly, blotong mushroom is often consumed by people but some were poisoned. The purpose of this study was to present a scientific data on blotong mushroom taxonomy and ensure the toxic content of blotong mushrooms based on the difference in age according to Brine Shrimp Lethality Test (BST) method. Identification carried to genus level by macroscopic and microscopic observations based on the book of Identify Mushrooms To Genus I and VI. While the toxicity test was conducted by BST following the extraction method using ethanol 99%, preparation test solutions, toxicity test of mushroom extract using the larvae of Artemia salina L and data analysis using a probit analysis then compared through variance T test with 95% confidence levelThe identification showed that blotong mushroom belonged to genus Leopita with specific features such us the cap had scales, the stem had a ring that appears in adulthood and the spore form of amyloid. The toxicity test of probit analysis showed that blotong mushroom is toxic on different ages such us mushroom with closed cap and opened cap had an average LC50 of 234.709 mg/ml and LC50 of 583.902 mg/ml, respecvtively, according to BST result. While T Test showed the significance value of 0.273 0.05, it means that there is no significant difference between the toxic content on mushrooms blotong with age difference.Keywords: identification, blotong mushroom, toxic, Brine Shrimp Lethality Test
{"title":"Identifikasi dan Uji Toksisitas Ekstrak Jamur Blotong dengan Metode Brine Shrimp Lethality Test (BST)","authors":"Siti Nur Azizah, Lutfiya Cahyani, Reni Budiarti, Rudju Winarsa","doi":"10.53864/jifakfar.v1i2.14","DOIUrl":"https://doi.org/10.53864/jifakfar.v1i2.14","url":null,"abstract":"Blotong mushroom is a fungus that thrives in waste of filter cake (a solid waste of sugar mill) from PG Semboro, Jember Regency.Commonly, blotong mushroom is often consumed by people but some were poisoned. The purpose of this study was to present a scientific data on blotong mushroom taxonomy and ensure the toxic content of blotong mushrooms based on the difference in age according to Brine Shrimp Lethality Test (BST) method. Identification carried to genus level by macroscopic and microscopic observations based on the book of Identify Mushrooms To Genus I and VI. While the toxicity test was conducted by BST following the extraction method using ethanol 99%, preparation test solutions, toxicity test of mushroom extract using the larvae of Artemia salina L and data analysis using a probit analysis then compared through variance T test with 95% confidence levelThe identification showed that blotong mushroom belonged to genus Leopita with specific features such us the cap had scales, the stem had a ring that appears in adulthood and the spore form of amyloid. The toxicity test of probit analysis showed that blotong mushroom is toxic on different ages such us mushroom with closed cap and opened cap had an average LC50 of 234.709 mg/ml and LC50 of 583.902 mg/ml, respecvtively, according to BST result. While T Test showed the significance value of 0.273 0.05, it means that there is no significant difference between the toxic content on mushrooms blotong with age difference.Keywords: identification, blotong mushroom, toxic, Brine Shrimp Lethality Test","PeriodicalId":17737,"journal":{"name":"JURNAL ILMIAH FARMASI AKADEMI FARMASI JEMBER","volume":"86 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-01-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90330537","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-01-28DOI: 10.53864/jifakfar.v2i1.19
Yulianto Ade Prasetya, Khoirun Nisyak
Lactobacillus acidophillus dalam bentuk yoghurt mempunyai efek penurunan terhadap hiperkolesterolemia namun dapat mengalami stress akibat asam lambung. Teknik bioenkapsulasi mampu meningkatkan viabilitas dan perlindungan terhadap bakteri asam laktat tersebut. Penelitian ini bertujuan untuk mengetahui bahan bioenkapsulasi L. acidophillus dari Whey Protein Isolate (WPI), kasein, gum arab, dan amilum yang terbaik viabilitasnya terhadap asam lambung dan mengetahui dosis bioenkapsulasi yoghurt (single, double atau triple dose) yang efektif sebagai terapi antihiperkolesterolemia. Viabilitas bioenkapsulasi yoghurt terhadap asam lambung dilakukan secara in vitro dengan teknik Total Plate Count (TPC) kemudian diujikan aktivitas antihiperkolesterolemia secara in vivo terhadap tikus putih galur wistar. Hasil menunjukkan bahwa bahan dari amilum menunjukkan hasil yang terbaik dalam peningkatan viabilitas sebelum dan sesudah diberi perlakuan asam lambung yakni dari 3.0 x106 CFU/g menjadi 2.1 x 108 CFU/g. Dosis yang efektif sebagai antihiperkolesterolemia pada tikus putih wistar yakni single dose dengan nilai rataan kadar Low Density Lipoprotein (LDL) sebesar 15mg.dL-1.Kata kunci: bioenkapsulasi, hiperkolesterolemia, Lactobacillus acidophillus, yoghurt
{"title":"BIOENKAPSULASI YOGHURT Lactobacillus acidophillus SEBAGAI ANTIHIPERKOLESTROLEMIA","authors":"Yulianto Ade Prasetya, Khoirun Nisyak","doi":"10.53864/jifakfar.v2i1.19","DOIUrl":"https://doi.org/10.53864/jifakfar.v2i1.19","url":null,"abstract":"Lactobacillus acidophillus dalam bentuk yoghurt mempunyai efek penurunan terhadap hiperkolesterolemia namun dapat mengalami stress akibat asam lambung. Teknik bioenkapsulasi mampu meningkatkan viabilitas dan perlindungan terhadap bakteri asam laktat tersebut. Penelitian ini bertujuan untuk mengetahui bahan bioenkapsulasi L. acidophillus dari Whey Protein Isolate (WPI), kasein, gum arab, dan amilum yang terbaik viabilitasnya terhadap asam lambung dan mengetahui dosis bioenkapsulasi yoghurt (single, double atau triple dose) yang efektif sebagai terapi antihiperkolesterolemia. Viabilitas bioenkapsulasi yoghurt terhadap asam lambung dilakukan secara in vitro dengan teknik Total Plate Count (TPC) kemudian diujikan aktivitas antihiperkolesterolemia secara in vivo terhadap tikus putih galur wistar. Hasil menunjukkan bahwa bahan dari amilum menunjukkan hasil yang terbaik dalam peningkatan viabilitas sebelum dan sesudah diberi perlakuan asam lambung yakni dari 3.0 x106 CFU/g menjadi 2.1 x 108 CFU/g. Dosis yang efektif sebagai antihiperkolesterolemia pada tikus putih wistar yakni single dose dengan nilai rataan kadar Low Density Lipoprotein (LDL) sebesar 15mg.dL-1.Kata kunci: bioenkapsulasi, hiperkolesterolemia, Lactobacillus acidophillus, yoghurt","PeriodicalId":17737,"journal":{"name":"JURNAL ILMIAH FARMASI AKADEMI FARMASI JEMBER","volume":"18 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-01-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85448693","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-01-28DOI: 10.53864/jifakfar.v1i2.125
Indah Muflihatin
Usia balita terutama pada usia 1-5 tahun merupakan masa pertumbuhan yang cepat baik fisik maupun otak. Sehingga memerlukan kebutuhan gizi yang paling banyak, pada masa ini anak sering mengalami kesulitan makan, apabila kebutuhan nutrisi tidak ditangani dengan baik maka akan mudah terjadi Kekurangan Energi Protein. Penelitian ini dilakukan untuk mengetahui pengaruh status gizi terhadap pertumbuhan dan perkembangan balita usia 1-5 tahun di PAUD candijati. Pada penelitian ini menggunakan jenis penelitian analitik dengan desain cross sectional. Populasi dalam penelitian ini adalah 15 balita, dalam pengambilan sampelnya menggunakan teknik total sampling dengan sampel sebanyak 15 balita. Pengumpulan data menggunakan kuesioner KPSP kemudian dianalisis dengan uji chi square. Berdasarkan hasil uji statistik dengan menggunakan chi-square didapatkan nilai sell yang memiliki nilai expected kurang dari 5 adalah 6 sell (100%), sehingga tidak memenuhi syarat untuk dilakukan uji chi-square (jumlah sell dengan nilai expected kurang dari 5 tidak boleh lebih dari 20%), sehingga di gunakan uji exact dari fisher atau disebut juga fisher exact test. Dengan menggunakan software analisis statistik didapatkan hasil nilai value = 2.315 dan nilai sig.=0.5. Dengan demikian karena nilai sig. (0.5) >α (0.05) maka H0 di terima, artinya tidak ada pengaruh status gizi terhadap pertumbuhan dan perkembangan pada balita usia 1-5 tahun di PAUD Candijati.Diharapkan kesadaran petugas kesehatan untuk memberikan penyuluhan sangat berperan dalam mengubah perilaku masyarakat dalam menurunkan angka kejadian Kekurangan Energi Protein.
{"title":"PENGARUH STATUS GIZI TERHADAP PERTUMBUHAN DAN PERKEMBANGAN PADA BALITA USIA 1–5 TAHUN DI PAUD CANDIJATI TAHUN 2015","authors":"Indah Muflihatin","doi":"10.53864/jifakfar.v1i2.125","DOIUrl":"https://doi.org/10.53864/jifakfar.v1i2.125","url":null,"abstract":"Usia balita terutama pada usia 1-5 tahun merupakan masa pertumbuhan yang cepat baik fisik maupun otak. Sehingga memerlukan kebutuhan gizi yang paling banyak, pada masa ini anak sering mengalami kesulitan makan, apabila kebutuhan nutrisi tidak ditangani dengan baik maka akan mudah terjadi Kekurangan Energi Protein. Penelitian ini dilakukan untuk mengetahui pengaruh status gizi terhadap pertumbuhan dan perkembangan balita usia 1-5 tahun di PAUD candijati. Pada penelitian ini menggunakan jenis penelitian analitik dengan desain cross sectional. Populasi dalam penelitian ini adalah 15 balita, dalam pengambilan sampelnya menggunakan teknik total sampling dengan sampel sebanyak 15 balita. Pengumpulan data menggunakan kuesioner KPSP kemudian dianalisis dengan uji chi square. Berdasarkan hasil uji statistik dengan menggunakan chi-square didapatkan nilai sell yang memiliki nilai expected kurang dari 5 adalah 6 sell (100%), sehingga tidak memenuhi syarat untuk dilakukan uji chi-square (jumlah sell dengan nilai expected kurang dari 5 tidak boleh lebih dari 20%), sehingga di gunakan uji exact dari fisher atau disebut juga fisher exact test. Dengan menggunakan software analisis statistik didapatkan hasil nilai value = 2.315 dan nilai sig.=0.5. Dengan demikian karena nilai sig. (0.5) >α (0.05) maka H0 di terima, artinya tidak ada pengaruh status gizi terhadap pertumbuhan dan perkembangan pada balita usia 1-5 tahun di PAUD Candijati.Diharapkan kesadaran petugas kesehatan untuk memberikan penyuluhan sangat berperan dalam mengubah perilaku masyarakat dalam menurunkan angka kejadian Kekurangan Energi Protein.","PeriodicalId":17737,"journal":{"name":"JURNAL ILMIAH FARMASI AKADEMI FARMASI JEMBER","volume":"55 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-01-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73584999","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-01-28DOI: 10.53864/jifakfar.v1i2.13
Rosida, Sri Winarsih, Diyan Ajeng R.
Kulit buah pisang merupakan salah satu bahan alami yang dapat digunakan dalam proses penyembuhan luka bakar. Hal ini disebabkan adanya kandungan flavonoid yang memiliki aktivitas antioksidan sehingga dapat mempercepat fase inflamasi dan meningkatkan sintesis kolagen. Tujuan dari penelitian ini membuktikan ekstrak etanol kulit buah pisang (Musa acuminata Colla) dalam proses penyembuhan luka bakar pada tikus galur wistar penderita diabetes. Metode penelitian menggunakan desain pre and post control group. Sebanyak enam belas ekor tikus dibagi menjadi 4 kelompok perlakuan. Kelompok I (kontrol negatif) diberi basis, kelompok II, III dan IV (kelompok perlakuan) diberi ekstrak etanol kulit buah pisang dengan dosis 25%, 50% dan 75%. Sebelum dibuat model luka bakar, tikus diinduksi aloksan dengan dosis 150 mg/kg BB selama tiga hari. Tikus yang digunakan sebagai model luka bakar adalah tikus dengan kadar gula darah diatas 200 mg/dL. Model luas luka bakar sebesar 225 mm2. Pemberian ekstrak kulit buah pisang secara topikal dilakukan tiap hari selama 16 hari. Pengukuran luas luka diamati pada hari ke 4, 8, 12 dan 16. Hasil penelitian menunjukkan bahwa ekstrak kulit buah pisang (Musa acuminata Colla) berpotensi sebagai obat luka bakar pada tikus putih penderita diabetes (p 0,05).Kata Kunci : Musa acuminata, Luka bakar, Diabetes
{"title":"POTENSI EKSTRAK ETANOL KULIT BUAH PISANG (Musa acuminata Colla) SEBAGAI OBAT LUKA BAKAR TERHADAP TIKUS GALUR WISTAR PENDERITA DIABETES","authors":"Rosida, Sri Winarsih, Diyan Ajeng R.","doi":"10.53864/jifakfar.v1i2.13","DOIUrl":"https://doi.org/10.53864/jifakfar.v1i2.13","url":null,"abstract":"Kulit buah pisang merupakan salah satu bahan alami yang dapat digunakan dalam proses penyembuhan luka bakar. Hal ini disebabkan adanya kandungan flavonoid yang memiliki aktivitas antioksidan sehingga dapat mempercepat fase inflamasi dan meningkatkan sintesis kolagen. Tujuan dari penelitian ini membuktikan ekstrak etanol kulit buah pisang (Musa acuminata Colla) dalam proses penyembuhan luka bakar pada tikus galur wistar penderita diabetes. Metode penelitian menggunakan desain pre and post control group. Sebanyak enam belas ekor tikus dibagi menjadi 4 kelompok perlakuan. Kelompok I (kontrol negatif) diberi basis, kelompok II, III dan IV (kelompok perlakuan) diberi ekstrak etanol kulit buah pisang dengan dosis 25%, 50% dan 75%. Sebelum dibuat model luka bakar, tikus diinduksi aloksan dengan dosis 150 mg/kg BB selama tiga hari. Tikus yang digunakan sebagai model luka bakar adalah tikus dengan kadar gula darah diatas 200 mg/dL. Model luas luka bakar sebesar 225 mm2. Pemberian ekstrak kulit buah pisang secara topikal dilakukan tiap hari selama 16 hari. Pengukuran luas luka diamati pada hari ke 4, 8, 12 dan 16. Hasil penelitian menunjukkan bahwa ekstrak kulit buah pisang (Musa acuminata Colla) berpotensi sebagai obat luka bakar pada tikus putih penderita diabetes (p 0,05).Kata Kunci : Musa acuminata, Luka bakar, Diabetes","PeriodicalId":17737,"journal":{"name":"JURNAL ILMIAH FARMASI AKADEMI FARMASI JEMBER","volume":"14 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-01-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81311517","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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