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Words from the FELASA President. FELASA 主席的话
IF 2.4 4区 农林科学 Q1 Veterinary Pub Date : 2024-04-01 DOI: 10.1177/00236772241242736
Klas Abelson
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引用次数: 0
Validation of computed tomography as a diagnostic tool in guinea pigs with non-alcoholic fatty liver disease. 计算机断层扫描作为非酒精性脂肪性肝病豚鼠诊断工具的验证
IF 2.4 4区 农林科学 Q1 Veterinary Pub Date : 2024-04-01 Epub Date: 2023-11-24 DOI: 10.1177/00236772231182511
Kristine Lintrup, David Højland Ipsen, Josephine Skat-Rørdam, Jens Lykkesfeldt, Pernille Tveden-Nyborg, Lene Elisabeth Buelund

Non-alcoholic fatty liver disease (NAFLD) and subsequent steatohepatitis (NASH) is the most common cause of liver disease and liver transplantation in humans. Affecting millions of patients worldwide, diagnosis relies on a biopsy, not without risk to the patient, and emphasises the need for improved diagnostic measures to determine and monitor disease progression. Despite intensive research, approved pharmacological treatment modalities are few, underlining that animal models with increased translational validity are important to advance preclinical drug development. This study validates the applicability of computed tomography (CT) as a non-invasive diagnostic tool for the assessment of liver steatosis in a guinea pig model of NAFLD/NASH. Guinea pigs with induced NAFLD or NASH were compared to healthy controls at two separate time points: week 16, serving as baseline measure, and week 25 to monitor disease progression over time. The animals were subsequently euthanised, and samples were collected to confirm disease stage. The data showed a strong negative correlation between liver triglycerides and Hounsfield unit (HU) values (R2 = 0.8157; p < 0.0001). A significant difference in histopathological scoring and HU values between grade 0 and more advanced stages of steatosis was recorded (p < 0.001), although the degree of liver fibrosis could not be accurately evaluated by differences in HU. In conclusion, the present study validates CT scanning for the determination of hepatic steatosis in guinea pigs, and it strongly supports the technique as a relevant non-invasive diagnostic tool in this species.

非酒精性脂肪性肝病(NAFLD)和随后的脂肪性肝炎(NASH)是人类肝脏疾病和肝移植的最常见原因。该病影响着全世界数百万患者,诊断依赖于活检,对患者并非没有风险,并强调需要改进诊断措施,以确定和监测疾病进展。尽管进行了深入的研究,但批准的药物治疗方式很少,这表明具有更高翻译有效性的动物模型对推进临床前药物开发非常重要。本研究在豚鼠NAFLD/NASH模型中验证了计算机断层扫描(CT)作为评估肝脏脂肪变性的非侵入性诊断工具的适用性。在两个不同的时间点,将诱导NAFLD或NASH的豚鼠与健康对照进行比较:第16周作为基线测量,第25周监测疾病随时间的进展。随后对这些动物实施安乐死,并收集样本以确认疾病阶段。数据显示肝脏甘油三酯与Hounsfield单位(HU)值呈显著负相关(R2 = 0.8157;p p
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引用次数: 0
Watch out for five felasa working group recommendations. 关注 felasa 工作组的五项建议。
IF 2.4 4区 农林科学 Q1 Veterinary Pub Date : 2024-04-01 DOI: 10.1177/00236772241242729
Livia D'Angelo
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引用次数: 0
Eugenol as anesthetic for Cnesterodon decemmaculatus (Cyprinodontiformes, Poeciliidae). Eugenol作为去甲Cnesterodon decemmaculatus(Cyprinodonformes,Poecilidae)的麻醉剂。
IF 2.4 4区 农林科学 Q1 Veterinary Pub Date : 2024-02-01 Epub Date: 2023-09-15 DOI: 10.1177/00236772231192020
Maria C Vercellini, Ramiro Rearte, Luca di Cesare, Miguel A Ayala, Martin M Montes

Anesthetics are commonly used in fish for surgery and to facilitate capture, handling and transport in aquaculture and experimental procedures. In research, the selection of the anesthetic depends on its properties and on the recovery time. Eugenol has been pointed out as an effective anesthetic for fish, alternative to traditional drugs. Although Cnesterodon decemmaculatus is widely used as a model in ecological bioassays, no anesthetic protocol has been reported for this species. The aim of the present study was to evaluate the induction time (i.e. time to reach anesthetic stage VI) and recovery time in individuals of C. decemmaculatus subjected to eugenol at a fixed concentration, according to sex and pregnancy status. Forty-one fish were divided into three groups: males, pregnant females and non-pregnant females. They were measured for total length, standard length and weight, and the condition factor (K) was calculated. No significant differences in induction and recovery times were found for sex, pregnancy status and K between groups. Results are a contribution toward the development protocol of a standard anesthetic protocol for C. decemmaculatus.

在水产养殖和实验过程中,麻醉剂通常用于鱼类的外科手术以及便于捕获、处理和运输。在研究中,麻醉剂的选择取决于其特性和恢复时间。有人指出,丁香酚是一种有效的鱼类麻醉剂,可替代传统药物。虽然 Cnesterodon decemmaculatus 被广泛用作生态生物测定的模型,但还没有关于该物种麻醉方案的报道。本研究的目的是根据鱼的性别和怀孕状况,评估鱼类个体在固定浓度丁香酚作用下的诱导时间(即达到麻醉阶段 VI 的时间)和恢复时间。41 条鱼被分为三组:雄鱼、怀孕雌鱼和未怀孕雌鱼。测量它们的总长度、标准长度和体重,并计算条件因子(K)。各组之间的诱导和恢复时间在性别、怀孕状态和 K 方面均无明显差异。研究结果有助于为栉水母制定标准麻醉方案。
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引用次数: 0
ERK1/2 modulation in the central nervous system of male and female thiamine-deficient mice with amprolium. 安普罗林对雌雄硫胺素缺乏小鼠中枢神经系统中 ERK1/2 的调节作用
IF 2.4 4区 农林科学 Q1 Veterinary Pub Date : 2024-02-01 Epub Date: 2023-09-08 DOI: 10.1177/00236772231191586
Mirian P da Silva, Francisco Wb Lima, Adha Gs Moura, Julia P Nunes, Clarissa As de Cordova, Fabiano M de Cordova

Thiamine deficiency experimental models focus on using the pyrithiamine analog in male rodents, making the thiamine deficiency effects in females and the use of other thiamine antagonists, such as amprolium, unknown. We investigated the impact of thiamine deficiency with amprolium in the cerebral cortex and thalamus of male and female mice by evaluating the modulation of ERK1/2 phosphorylation. The animals were exposed for 20 days to thiamine-deficient chow with different doses of amprolium (20, 40, 60 and 80 mg/kg) and at different treatment periods (five, 10, 15 or 20 days) at a dose of 60 mg/kg. After treatments, ERK1/2 phosphorylation was analyzed by western blot. In male mice, we observed a progressive increase in ERK1/2 phosphorylation in both the cerebral cortex and thalamus in response to the dose of amprolium. In females, ERK1/2 phosphorylation did not progressively increase in response to the amprolium dosage. However, an increase in phosphorylation at the higher doses of 60 and 80 mg/kg was observed. We observed a more intense increase in ERK1/2 phosphorylation in males' cerebral cortex and thalamus from 10 days onwards. In females, the ERK1/2 modulation profiles were similar. The results show that thiamine deficiency induction with amprolium is efficient, compatible with other recognized models that use pyrithiamine, showing changes in cell signaling in the nervous system. The study showed differences in response to thiamine deficiency with amprolium between male and female mice in relation to ERK1/2 phosphorylation and demonstrated that females respond positively to the method and can also be used as model animals.

硫胺素缺乏的实验模型主要是在雄性啮齿类动物中使用吡硫胺素类似物,因此雌性小鼠硫胺素缺乏的影响以及使用其他硫胺素拮抗剂(如安普罗林)的情况尚不清楚。我们通过评估ERK1/2磷酸化的调节情况,研究了雌雄小鼠大脑皮层和丘脑中使用氨溴索对硫胺素缺乏的影响。将动物暴露于硫胺素缺乏的饲料中 20 天,同时添加不同剂量的安普列林(20、40、60 和 80 毫克/千克),并在不同的处理期(5、10、15 或 20 天)添加剂量为 60 毫克/千克的安普列林。治疗后,ERK1/2 磷酸化情况通过 Western 印迹进行分析。在雄性小鼠中,我们观察到大脑皮层和丘脑中的ERK1/2磷酸化随着安络血剂量的增加而逐渐增加。在雌性小鼠中,ERK1/2磷酸化并没有随着安瓿剂量的增加而逐渐增加。然而,在 60 和 80 毫克/千克的较高剂量下,磷酸化增加。我们观察到,从10天开始,雄性大脑皮层和丘脑中的ERK1/2磷酸化增加更为剧烈。在雌性动物中,ERK1/2的调节情况类似。结果表明,使用安瓿诱导硫胺素缺乏症是有效的,与其他公认的使用吡硫胺素的模型相一致,显示了神经系统细胞信号的变化。研究表明,雄性和雌性小鼠对使用安瓿诱导硫胺素缺乏症的反应在ERK1/2磷酸化方面存在差异,并证明雌性小鼠对该方法反应积极,也可用作模型动物。
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引用次数: 0
XVII SECAL CONGRESS Santiago de Compostela (Spain), 14-17 November 2023. 第十七届 SECAL 大会 圣地亚哥-德孔波斯特拉(西班牙),2023 年 11 月 14-17 日。
IF 2.4 4区 农林科学 Q1 Veterinary Pub Date : 2024-02-01 DOI: 10.1177/00236772231225388
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引用次数: 0
Investigation of circulating infectious agents in experimental Beagle dogs of a production colony and three research facilities in China from June 2021 to May 2022. 2021 年 6 月至 2022 年 5 月期间对中国一个生产基地和三个研究机构的实验用比格犬体内循环感染病原体的调查。
IF 2.4 4区 农林科学 Q1 Veterinary Pub Date : 2024-02-01 Epub Date: 2023-09-13 DOI: 10.1177/00236772231188172
Yue Zhao, Panlong Wang, Xueting Qu, Kunpeng Yuan, Suzhen Zhu, Sen Wang, Qingdong Luan, Hao Zhou, Yue Yin, Zijing Zhao, Yongjuan Gao, Shuzhen Chen, Yanjing Lu, Jianlin Wang, Yanbo Yin

To understand the epizootiologic characteristics of pathogens and opportunistic infections in one Beagle dog production colony and three research facilities, viruses and mycoplasma were detected in 1777 samples collected from Beagle dogs in China by polymerase chain reaction/reverse transcription polymerase chain reaction, and bacteria were isolated and identified by 16S rRNA sequence analysis. In addition, genotyping of the major circulating viruses was carried out by amplification of gene fragments and homology analysis. Canine coronavirus (CCoV), Escherichia coli, canine parvovirus (CPV), Bordetella bronchiseptica, Clostridium perfringens, Mycoplasma cynos, Klebsiella pneumoniae, Streptococcus canis, canine astrovirus (CaAstV), canine kobuvirus (CaKV), Pseudomonas aeruginosa, Proteus mirabilis, Macrococcus canis, Pasteurella canis, canine bocavirus (CBoV) and canine adenovirus (CAdV) were detected in the samples. Single, double, triple and quadruple infections accounted for 6.6%, 1.4%, 1.2% and 0.96% of samples, respectively. CCoV strains in 81 samples included three genotypes, CCoV-I, CCoV-IIa and CCoV-IIb, by analysis of S gene. The rate of single infection of CCoV-I, CCoV-IIa or CCoV-IIb was 19%, 38% or 7.4% respectively. The double and triple infection rates of CCoV were 32.8% and 2.5% respectively. All CPV strains in 36 samples belonged to CPV-2c. There were three amino acid differences in the Fiber protein of CAdV-positive sample QD2022, compared with the reference strain Toronto A26/61 and the vaccine strain YCA-18. These results suggest that CCoV and CPV are primary infectious agents, and that these two viruses were often identified in mixed infections, or coinfections alongside mycoplasma or other bacteria. These results will provide the basis for improvements in prevention and control of naturally occurring infectious diseases in Beagle dog production colonies and research facilities.

为了了解一个比格犬生产群和三个研究机构中病原体和机会性感染的流行病学特征,我们通过聚合酶链式反应/反转录聚合酶链式反应检测了从中国比格犬身上采集的 1777 份样本中的病毒和支原体,并通过 16S rRNA 序列分析分离和鉴定了细菌。此外,还通过基因片段扩增和同源性分析对主要流行病毒进行了基因分型。犬冠状病毒 (CCoV)、大肠杆菌、犬细小病毒 (CPV)、支气管败血波氏杆菌、产气荚膜梭菌、犬支原体、肺炎克雷伯菌、犬链球菌、犬星状病毒 (CaAstV)、在样本中检测到铜绿假单胞菌、奇异变形杆菌、犬巨球菌、犬巴氏杆菌、犬细小病毒(CBoV)和犬腺病毒(CAdV)。单一感染、双重感染、三重感染和四重感染分别占样本的 6.6%、1.4%、1.2% 和 0.96%。通过分析 S 基因,81 个样本中的 CCoV 株系包括 CCoV-I、CCoV-IIa 和 CCoV-IIb 三种基因型。CCoV-I、CCoV-IIa 或 CCoV-IIb 的单一感染率分别为 19%、38% 或 7.4%。CCoV的双重和三重感染率分别为32.8%和2.5%。36 个样本中的所有 CPV 株系均属于 CPV-2c。与参考毒株多伦多 A26/61 和疫苗毒株 YCA-18 相比,CAdV 阳性样本 QD2022 的纤维蛋白有三个氨基酸差异。这些结果表明,CCoV 和 CPV 是主要传染源,这两种病毒经常在混合感染或与支原体或其他细菌同时感染时被发现。这些结果将为改进比格犬生产群和研究设施中自然发生的传染病的预防和控制提供依据。
{"title":"Investigation of circulating infectious agents in experimental Beagle dogs of a production colony and three research facilities in China from June 2021 to May 2022.","authors":"Yue Zhao, Panlong Wang, Xueting Qu, Kunpeng Yuan, Suzhen Zhu, Sen Wang, Qingdong Luan, Hao Zhou, Yue Yin, Zijing Zhao, Yongjuan Gao, Shuzhen Chen, Yanjing Lu, Jianlin Wang, Yanbo Yin","doi":"10.1177/00236772231188172","DOIUrl":"10.1177/00236772231188172","url":null,"abstract":"<p><p>To understand the epizootiologic characteristics of pathogens and opportunistic infections in one Beagle dog production colony and three research facilities, viruses and mycoplasma were detected in 1777 samples collected from Beagle dogs in China by polymerase chain reaction/reverse transcription polymerase chain reaction, and bacteria were isolated and identified by 16S rRNA sequence analysis. In addition, genotyping of the major circulating viruses was carried out by amplification of gene fragments and homology analysis. Canine coronavirus (CCoV), <i>Escherichia coli</i>, canine parvovirus (CPV), <i>Bordetella bronchiseptica</i>, <i>Clostridium perfringens</i>, <i>Mycoplasma cynos</i>, <i>Klebsiella pneumoniae</i>, <i>Streptococcus canis</i>, canine astrovirus (CaAstV), canine kobuvirus (CaKV), <i>Pseudomonas aeruginosa</i>, <i>Proteus mirabilis</i>, <i>Macrococcus canis</i>, <i>Pasteurella canis</i>, canine bocavirus (CBoV) and canine adenovirus (CAdV) were detected in the samples. Single, double, triple and quadruple infections accounted for 6.6%, 1.4%, 1.2% and 0.96% of samples, respectively. CCoV strains in 81 samples included three genotypes, CCoV-I, CCoV-IIa and CCoV-IIb, by analysis of S gene. The rate of single infection of CCoV-I, CCoV-IIa or CCoV-IIb was 19%, 38% or 7.4% respectively. The double and triple infection rates of CCoV were 32.8% and 2.5% respectively. All CPV strains in 36 samples belonged to CPV-2c. There were three amino acid differences in the Fiber protein of CAdV-positive sample QD2022, compared with the reference strain Toronto A26/61 and the vaccine strain YCA-18. These results suggest that CCoV and CPV are primary infectious agents, and that these two viruses were often identified in mixed infections, or coinfections alongside mycoplasma or other bacteria. These results will provide the basis for improvements in prevention and control of naturally occurring infectious diseases in Beagle dog production colonies and research facilities.</p>","PeriodicalId":18013,"journal":{"name":"Laboratory Animals","volume":null,"pages":null},"PeriodicalIF":2.4,"publicationDate":"2024-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10279176","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Clinical efficacy of buprenorphine after oral dosing in rats undergoing major surgery. 大手术大鼠口服丁丙诺啡的临床疗效。
IF 2.4 4区 农林科学 Q1 Veterinary Pub Date : 2024-02-01 Epub Date: 2023-09-05 DOI: 10.1177/00236772231178417
Einar Sjaastad Nordén, Ioanni Veras, Prakash Yadav, Kari Løken, Hilde Dishington, Christian Thorstensen, Ivar Sjaastad, Henrik Rasmussen

Serum corticosterone, serum buprenorphine, body weight change, consumption of food and water and behaviour-based pain assessment were measured in catheterised and non-catheterised male Wistar rats undergoing myocardial infarct (MI) surgery under general anaesthesia following buprenorphine dosing by subcutaneous (Bup-SC, 0.05 mg/kg) and oral (Bup-O, 0.4 mg/kg) routes. Buprenorphine was dosed subcutaneously at half an hour before and 8, 16 and 24 hours after surgery (Bup-SC), orally at one hour before surgery (Bup-O1) or at one hour before and 12 hours after surgery (Bup-O2) in catheterised rats and at one hour before and 24 hours after surgery (Bup-O24) in non-catheterised rats. Serum corticosterone, body weight changes and food and water consumption were not significantly different between treatments in catheterised rats. Bup-SC resulted in rapidly decreasing serum concentrations below the clinically effective concentrations (1 ng/mL) already at two hours after the first dose. Bup-O provided significantly higher and slowly decreasing serum concentrations, at or above clinically effective concentrations, for 24 hours (Bup-O1) and 42 hours (Bup-O2) after surgery. In non-catheterised rats, body weight development and food consumption were significantly higher in Bup-O24 rats compared to Bup-SC rats. The results indicate that a SC buprenorphine dose of 0.05 mg/kg every eight hours provides long periods of serum concentrations below clinically effective levels, and that a higher dose and/or more frequent dosage are required to provide stable serum concentrations at or above clinically effective levels. A single oral buprenorphine dose of 0.4 mg/kg provides clinically effective and stable serum concentrations for 24 hours in rats after MI surgery.

通过皮下注射(Bup-SC,0.05 毫克/千克)和口服(Bup-O,0.4 毫克/千克)丁丙诺啡的途径,测量了在全身麻醉下接受心肌梗塞(MI)手术的导管和非导管雄性 Wistar 大鼠的血清皮质酮、血清丁丙诺啡、体重变化、食物和水消耗量以及基于行为的疼痛评估。对导管插入的大鼠,在手术前半小时和手术后 8、16 和 24 小时皮下注射丁丙诺啡(Bup-SC);对未插入导管的大鼠,在手术前一小时口服丁丙诺啡(Bup-O1)或在手术前一小时和手术后 12 小时口服丁丙诺啡(Bup-O2);对未插入导管的大鼠,在手术前一小时和手术后 24 小时口服丁丙诺啡(Bup-O24)。导尿大鼠的血清皮质酮、体重变化以及食物和水消耗量在不同处理之间没有显著差异。Bup-SC 可使血清中的皮质酮浓度迅速下降,在首次给药后两小时已低于临床有效浓度(1 纳克/毫升)。术后 24 小时(Bup-O1)和 42 小时(Bup-O2),Bup-O 可使血清浓度明显升高并缓慢下降,达到或超过临床有效浓度。与 Bup-SC 大鼠相比,未接受导管插入手术的 Bup-O24 大鼠的体重增长和食物消耗量明显更高。结果表明,每 8 小时一次 0.05 毫克/千克的丁丙诺啡皮下注射剂量可使血清浓度长期低于临床有效水平,需要更大的剂量和/或更频繁的剂量才能使血清浓度稳定在或高于临床有效水平。在心肌梗死手术后的大鼠身上,单次口服 0.4 毫克/千克的丁丙诺啡剂量可在 24 小时内提供临床有效和稳定的血清浓度。
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引用次数: 0
Evaluating rearing behaviour as a model-specific pain indicator in mouse osteotomy models. 评估小鼠截骨模型中作为模型特异性疼痛指标的饲养行为。
IF 2.4 4区 农林科学 Q1 Veterinary Pub Date : 2024-02-01 Epub Date: 2023-09-08 DOI: 10.1177/00236772231183197
Angelique Wolter, Paulin Jirkof, Christa Thöne-Reineke, Anna E Rapp, Annemarie Lang

To assess pain in mouse models of bone fractures, currently applied assessment batteries use combinations of clinical signs with spontaneous behaviours and model-specific behaviours, including walking and weight-bearing behaviour. Rearing behaviour - an upright position on the hindlimbs - has a motivational and an ambulatory component. Thus, rearing behaviour might have the potential to be an indicator for model-specific pain in mouse fracture models. To date, the assessment of rearing behaviour in bone fracture models using mice is only scarcely described. In this study, we aimed to determine whether the duration of rearing behaviour is affected by osteotomy of the femur in male and female C57BL/6N mice with external fixation (rigid vs. flexible) and could be an additional sign for model-specific pain, such as the presence of limping. Rearing duration was significantly decreased after osteotomy in male and female mice at 24 h, 48 h and 72 h, but was not affected by anaesthesia/analgesia alone. In male mice, the relative rearing duration increased over 72 h (both fixations) and at 10 days in the rigid fixation group but remained significantly lower in the flexible fixation group. In contrast, in female mice, no increase in rearing duration was observed within 72 h and at 10 days post-osteotomy, independent of the fixation. We did not identify any association between relative rearing time and presence or absence of limping. In summary, our results do not provide sufficient evidence that altered rearing behaviour might be an indicative sign for pain in this model.

为了评估骨折小鼠模型中的疼痛,目前使用的评估电池将临床症状与自发行为和模型特定行为(包括行走和负重行为)相结合。仰卧行为--后肢直立的姿势--具有动机和活动的成分。因此,仰卧行为有可能成为小鼠骨折模型中模型特异性疼痛的指标。迄今为止,对使用小鼠的骨折模型中仰卧行为的评估还鲜有描述。在这项研究中,我们的目的是确定在雌雄C57BL/6N小鼠股骨外固定(刚性与柔性)截骨术后,饲养行为的持续时间是否会受到影响,以及饲养行为是否可以作为模型特异性疼痛的额外标志,如是否出现跛行。截骨术后,雄性和雌性小鼠在24小时、48小时和72小时内的饲养持续时间明显缩短,但不受单独麻醉/镇痛的影响。在雄性小鼠中,刚性固定组的相对饲养持续时间在 72 小时内(两种固定方式)和 10 天内都有所增加,但在柔性固定组中仍明显较短。相反,在雌性小鼠中,无论采用哪种固定方式,在骨切除术后 72 小时内和 10 天内均未观察到饲养持续时间的增加。我们没有发现相对饲养时间与是否出现跛行之间有任何关联。总之,我们的研究结果没有提供足够的证据表明,在该模型中,饲养行为的改变可能是疼痛的一个指示性信号。
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引用次数: 0
Comparison of artery diameters in the Aachen minipig serving as a human intracranial in vivo model. 比较作为人类颅内活体模型的亚琛小型猪的动脉直径。
IF 2.4 4区 农林科学 Q1 Veterinary Pub Date : 2024-02-01 Epub Date: 2023-09-12 DOI: 10.1177/00236772231169809
Christoph Dorn, Lara Bender, Thorsten Sichtermann, Jan Minkenberg, Maximilian Franko, Ehsan Yousefian, Martin Wiesmann, Andrea Stockero, Rebecca May, Hani Ridwan, Omid Nikoubashman, Christiane Franz

Minipigs are used as in vivo endovascular models, particularly in stroke and aneurysm research. However, detailed knowledge of the diameters of forelimb arteries that are commonly used as surrogates for human brain-supplying arteries are lacking. This study aimed to determine the diameters of forelimb and neck arteries in Aachen minipigs and to compare those to the diameters of human cerebral brain-supplying arteries in order to assess the validity of the Aachen minipig as a human intracranial in vivo model. We measured the diameters in the external carotid artery and eight different branches of the subclavian artery in 12 Aachen minipigs using angiographic imaging. Analysed arteries comprised the external carotid artery, axillary artery, brachial artery, subscapular artery first segment, subscapular artery second segment, external thoracic artery, caudal circumflex humeral artery, suprascapular artery and thoracodorsal artery. We compared these diameters to diameters of the following human brain-supplying arteries: terminal internal carotid artery (carotid-T and petrous segment), M1 segment of the middle cerebral artery, M2 segments of the middle cerebral artery, anterior cerebral artery, vertebral artery and basilar artery. Median diameters of porcine forelimb arteries ranged from 1.8 to 4.9 mm, and human brain supplying arteries ranged in diameter from 1.4 to 4.3 mm. Depending on the intended use, this allows porcine forelimb arteries to be selected which are statistically comparable to human brain-supplying vessels. In conclusion, we identified several equivalent arteries of the porcine subclavian branches that are comparable to human brain-supplying arteries. This may help to validate the minipig as a suitable in vivo model for neurovascular experiments.

迷你猪被用作体内血管内模型,尤其是在中风和动脉瘤研究中。然而,人们对通常用作人类脑供血动脉替代物的前肢动脉的直径缺乏详细了解。本研究旨在确定亚琛小型猪前肢和颈部动脉的直径,并将其与人类大脑供血动脉的直径进行比较,以评估亚琛小型猪作为人类颅内活体模型的有效性。我们利用血管成像技术测量了 12 只亚琛小型猪的颈外动脉和锁骨下动脉 8 个不同分支的直径。分析的动脉包括颈外动脉、腋动脉、肱动脉、肩胛下动脉第一段、肩胛下动脉第二段、胸外动脉、肱骨尾周动脉、肩胛上动脉和胸背动脉。我们将这些直径与以下人脑供血动脉的直径进行了比较:颈内动脉末端(颈内动脉 T 段和皮质段)、大脑中动脉 M1 段、大脑中动脉 M2 段、大脑前动脉、椎动脉和基底动脉。猪前肢动脉的中位直径从 1.8 毫米到 4.9 毫米不等,人脑供血动脉的直径从 1.4 毫米到 4.3 毫米不等。根据用途的不同,猪前肢动脉在统计学上可与人脑供血血管相媲美。总之,我们确定了猪锁骨下分支的几条等效动脉,它们与人脑供血动脉具有可比性。这可能有助于验证小型猪是否适合作为神经血管实验的体内模型。
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引用次数: 0
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Laboratory Animals
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