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Pathologic copulatory lock in a genetically engineered laboratory mouse breeding pair. 病理交配锁定在基因工程实验室小鼠繁殖对。
IF 1.3 4区 农林科学 Q2 VETERINARY SCIENCES Pub Date : 2023-12-01 Epub Date: 2023-04-18 DOI: 10.1177/00236772231168185
Glory Leung, Rodolfo Ricart-Arbona, Sebastien Monette, Neil S Lipman

A breeding pair of genetically engineered laboratory mice (Mus musculus) presented in apparent copulatory lock (coital tie). After anesthetizing the animals, gentle traction was used to separate the pair at which point a vaginal prolapse was detected and the penis was covered with black, firm, dry crusts and noted to have a solid pale, tan, firm cylindrical mass adhering to its glans. The vaginal prolapse was reduced and the female was returned to its cage. The male mouse had a severely distended bladder which could not be expressed and was euthanized. Histopathologic examination of the distal two-thirds of the penis revealed diffuse, acute coagulative necrosis. The mass adhered to the distal penis was a homogenous granular eosinophilic material consistent with a copulatory plug. While copulatory plugs and locks have been described in some rodent species, they have not been reported in laboratory mice. While the cause of the adherence of the plug to the penis could not be determined, we hypothesize that its adherence to both the penis and the vagina led to the lock and subsequently to ischemic necrosis of the distal penis.

一对基因工程实验小鼠(小家鼠)表现出明显的交配锁(交合)。麻醉动物后,用轻柔的牵引将它们分开,此时发现阴道脱垂,阴茎上覆盖着黑色、坚硬、干燥的结痂,并注意到有一个坚实的苍白、褐色、坚硬的圆柱形肿块附着在其龟头上。阴道脱垂减少,雌性被放回笼子。雄鼠膀胱严重膨胀,无法表达,被安乐死。阴茎远端三分之二的组织病理学检查显示弥漫性急性凝固性坏死。附着在阴茎远端的肿块是一种均匀的颗粒状嗜酸性物质,与性交塞一致。虽然在一些啮齿动物物种中描述了交配堵塞和锁,但在实验室小鼠中尚未报道。虽然无法确定堵塞阴茎的原因,但我们假设它同时粘附在阴茎和阴道上,导致阴茎远端锁定并随后缺血性坏死。
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引用次数: 0
The importance of methodological detail and conceptual context when presenting laboratory animal science education tools: The authors' reply. 在展示实验动物科学教育工具时,方法细节和概念背景的重要性:作者的答复。
IF 2.4 4区 农林科学 Q2 VETERINARY SCIENCES Pub Date : 2023-12-01 Epub Date: 2023-05-19 DOI: 10.1177/00236772231176351
Eliana M Cicale, Verónica Casanova, Eduardo Caturini, Marianela Ceol Retamal, María C Greco, Sofía Gimenez, Julián E Gulin
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引用次数: 0
Mouse isoflurane anesthesia using the drop method. 异氟醚滴注法麻醉小鼠。
IF 2.4 4区 农林科学 Q2 VETERINARY SCIENCES Pub Date : 2023-12-01 Epub Date: 2023-05-05 DOI: 10.1177/00236772231169550
Maya J Bodnar, Anna S Ratuski, Daniel M Weary

Anesthesia with isoflurane prior to carbon dioxide euthanasia is recommended as a refinement, but vaporizer access can be limited. An alternative to vaporizers is the 'drop' method, introducing a fixed volume of isoflurane into the induction chamber. Previous work suggests that isoflurane administered at a concentration of 5% via the drop method is effective but aversive to mice; lower concentrations have not been tested. We assessed mouse behavior and insensibility with induction using the drop method for isoflurane concentrations below 5%. Male Crl:CD-1 (ICR) mice (n = 27) were randomly allocated to one of three isoflurane concentrations: 1.7%, 2.7%, and 3.7%. During induction, measures of insensibility and stress-related behaviors were recorded. All mice reached a surgical plane of anesthesia, and mice exposed to higher concentrations did so more quickly; as concentrations increased from 1.7 to 2.7 and 3.7%, the time to recumbency (Least squares means ± SE: 120.5 s ± 8.1, 97.9 s ± 8.1, and 82.8 s ± 8.1, respectively), loss of righting reflex (149.1 s ± 8.5, 127.7 s ± 8.5, and 100.7 s ± 8.5, respectively), and loss of pedal withdrawal reflex (214.5 s ± 8.3, 172.2 s ± 8.3, and 146.4 s ± 8.3, respectively) all declined. Rearing was the most frequently performed stress-related behavior, and was most pronounced immediately following isoflurane administration for all treatments. Our results indicate that the drop method can be used to effectively anesthetize mice with isoflurane concentrations as low as 1.7%; future work should assess mouse aversion.

建议在二氧化碳安乐死之前用异氟醚麻醉,但可以限制蒸发器的使用。汽化器的另一种替代方法是“滴法”,将固定体积的异氟烷引入感应室。先前的研究表明,通过滴注法以5%浓度给药的异氟醚是有效的,但对小鼠有反感;更低的浓度还没有测试过。我们用滴法对浓度低于5%的异氟醚诱导小鼠的行为和不敏感进行了评估。雄性Crl:CD-1 (ICR)小鼠(n = 27)随机分配到三种异氟烷浓度:1.7%、2.7%和3.7%中的一种。在诱导过程中,记录麻木和压力相关行为的测量。所有小鼠都达到了手术麻醉平面,暴露于高浓度的小鼠更快达到手术麻醉平面;当浓度从1.7增加到2.7和3.7%时,平躺时间(最小二乘法平均值±SE分别为120.5 s±8.1、97.9 s±8.1和82.8 s±8.1)、翻正反射丧失(分别为149.1 s±8.5、127.7 s±8.5和1000.7 s±8.5)和退足反射丧失(分别为214.5 s±8.3、172.2 s±8.3和146.4 s±8.3)均有所下降。养育是最常见的与压力相关的行为,并且在所有治疗中使用异氟醚后立即最明显。结果表明,异氟醚浓度低至1.7%时,滴注法可有效麻醉小鼠;未来的工作应该评估老鼠厌恶。
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引用次数: 1
Non-surgical external jugular vein catheterization using an ear vein access in piglets. 仔猪耳静脉非手术颈外静脉置管。
IF 2.4 4区 农林科学 Q2 VETERINARY SCIENCES Pub Date : 2023-12-01 Epub Date: 2023-04-19 DOI: 10.1177/00236772231167224
S K Ringer, A Schmid, M Weiss, S Ohlerth, N Spielmann, N G Clausen

The objective of this study was to investigate the feasibility of external jugular vein catheterization through an ear vein in piglets. Forty-six sevoflurane-midazolam anaesthetized piglets were included. External jugular vein catheterization was conducted through the ear vein using the Seldinger technique. Part 1 (n = 27): optimal puncture site was based on the deltoid tuberosity as a landmark to reach the external jugular vein. The final position of the catheter was verified in 25 piglets using computer tomography. Catheterization time was recorded and patency of the catheter assessed by repeated blood sampling for up to 4 h. Part 2 (n = 19): ear vein catheterization was without taking into account any landmarks. Functionality for blood sampling was evaluated as described in part 1. Catheter advancement was possible in 25/27 and 18/19 piglets in parts 1 and 2, respectively. Median (range) time required for successful catheterization was 1.95 (1-10) min (n = 38). The deltoid tuberosity was a good landmark to reach the external jugular vein. But blood sampling was also possible through catheters ending slightly cranial to the external jugular vein. Despite successful catheter advancement, blood sampling was not possible from one catheter in each part of the study (total: two piglets). One of these catheters presented luminal damage, while the other one presented as normal after being removed from the animal. Summarizing, central vein catheterization through the ear vein was feasible in 93.5% and repeated blood sampling was possible in 89.1% of the piglets (n = 46).

本研究的目的是探讨经耳静脉置入仔猪颈外静脉的可行性。纳入46头七氟咪达唑仑麻醉仔猪。采用Seldinger技术经耳静脉置管颈外静脉。第一部分(n = 27):最佳穿刺位置以三角结节为标志到达颈外静脉。通过计算机断层扫描在25头仔猪中验证了导管的最终位置。记录置管时间,通过反复采血评估导管通畅程度,持续4小时。第2部分(n = 19):耳静脉置管不考虑任何标志。如第1部分所述,对血液采样的功能进行了评估。在第1部分和第2部分中,分别有25/27和18/19头仔猪可以将导管推进。成功置管所需时间中位数(范围)为1.95 (1-10)min (n = 38)。三角结节是到达颈外静脉的良好标志。但也可以通过导管进行血液取样,导管的末端略高于颅部,直至颈外静脉。尽管导管进展顺利,但不可能在研究的每一部分(总共2头仔猪)都从一根导管中采集血液。其中一根导管出现管腔损伤,而另一根在从动物身上取出后表现正常。综上所述,93.5%的仔猪可以通过耳静脉中心静脉置管,89.1%的仔猪(n = 46)可以重复采血。
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引用次数: 0
Refinement of systemic guinea pig deafening in hearing research: Sensorineural hearing loss induced by co-administration of kanamycin and furosemide via the leg veins. 听力研究中豚鼠全身性耳聋的改进:卡那霉素和速尿经腿静脉联合给药引起的感音神经性听力损失。
IF 2.4 4区 农林科学 Q2 VETERINARY SCIENCES Pub Date : 2023-12-01 Epub Date: 2023-04-18 DOI: 10.1177/00236772231167679
Wiebke Behrends, Daniel Ahrens, Jens P Bankstahl, Karl-Heinz Esser, Gerrit Paasche, Thomas Lenarz, Verena Scheper

Auditory disabilities have a large impact on the human population worldwide. Research into understanding and treating hearing disabilities has increased significantly in recent years. One of the most relevant animal species in this context is the guinea pig, which has to be deafened to study several of the hearing pathologies and develop novel therapies. Applying kanamycin subcutaneously and furosemide intravenously is a long-established method in hearing research, leading to permanent hearing loss without surgical intervention at the ear. The intravenous application of furosemide requires invasive surgery in the cervical area of the animals to expose the jugular vein, since a relatively large volume (1 ml per 500 g body weight) must be injected over a period of about 2.5 min. We have established a gentler alternative by applying the furosemide by puncture of the leg veins. For this, custom-made cannula-needle devices were built to allow the vein puncture and subsequent slow injection of the furosemide. This approach was tested in 11 guinea pigs through the foreleg via the cephalic antebrachial vein and through the hind leg via the saphenous vein. Frequency-specific hearing thresholds were measured before and after the procedure to verify normal hearing and successful deafening, respectively. The novel approach of systemic deafening was successfully implemented in 10 out of 11 animals. The Vena saphena was best suited to the application. Since the animals' condition, post leg vein application, was better in comparison to animals deafened by exposure of the Vena jugularis, the postulated refinement that reduced animal stress was deemed successful.

听力障碍对全世界的人口有很大的影响。近年来,对理解和治疗听力障碍的研究显著增加。在此背景下,最相关的动物物种之一是豚鼠,它必须失聪才能研究几种听力病理并开发新的治疗方法。在听力研究中,卡那霉素皮下注射和速尿静脉注射是一种长期存在的方法,可导致永久性听力丧失,而无需在耳部进行手术干预。静脉应用速尿需要在动物颈部进行侵入性手术,以暴露颈静脉,因为必须在大约2.5分钟的时间内注射相对较大的体积(每500g体重1ml)。我们已经建立了一个更温和的替代方案通过穿刺腿部静脉使用速尿。为此,定制的套管针装置被建造,以允许静脉穿刺和随后缓慢注射速尿。该入路在11只豚鼠身上进行了试验,分别经前腿经头前臂静脉和后腿经隐静脉入路。在手术前后分别测量频率特异性听力阈值,以验证听力正常和耳聋成功。这种新的系统性耳聋方法在11只动物中的10只动物中成功实施。隐静脉是最适合的应用。由于动物的情况,腿后静脉应用,比暴露颈静脉致聋的动物更好,因此假设的减少动物压力的改进被认为是成功的。
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引用次数: 0
Harmonisation of education, training and continuing professional development for laboratory animal caretakers, technicians and technologists: Report of the FELASA-EFAT Working Group. 协调实验动物管理员、技术人员和技术人员的教育、培训和持续专业发展:FELASA-EFAT工作组的报告。
IF 2.4 4区 农林科学 Q2 VETERINARY SCIENCES Pub Date : 2023-12-01 Epub Date: 2023-06-19 DOI: 10.1177/00236772231175550
Klas Sp Abelson, Charlie Chambers, Teresa De La Cueva, Glyn Fisher, Penny Hawkins, Vasileios Ntafis, Paul F Pohlig, T Pim Rooymans, Ana I Santos

Competent, confident and caring laboratory animal caretakers, technicians and technologists (LAS staff) are vital for good animal welfare, high-quality science and a secure Culture of Care. This requires high-quality education, training, supervision and continuing professional development (CPD) of LAS staff. However, there is a lack of harmonisation regarding how this education and training is conducted among European countries, and nor are there recommendations adapted to Directive 2010/63/EU. Therefore, FELASA and EFAT established a working group with the task of establishing recommendations for education, training and CPD for LAS staff. The working group established five different levels (LAS staff levels 0-4), defining the required level of competence and attitude, as well as suggesting educational requirements for reaching each level. Defining these levels should help to ensure that appropriate educational and CPD activities are in place, and to enable employers and LAS staff to determine the level and career stage attained. Furthermore, proper assessment of competencies and effective CPD schemes for all relevant staff should be established. Regulators should support this by setting standards for competence assessment and ensuring that they are consistently applied. In addition, establishments should involve the LAS staff in defining and developing the Culture of Care. The Animal Welfare Body should be involved and have oversight of education, training and CPD. These recommendations will contribute to harmonisation and increased quality of education, training and CPD, as well as provide clearer career pathways for LAS staff, helping to ensure high standards of animal welfare and science.

有能力、自信和有爱心的实验动物看护人员、技术人员和技术人员(LAS员工)对于良好的动物福利、高质量的科学和安全的关怀文化至关重要。这就需要对阿联工作人员进行高质量的教育、培训、监督和持续专业发展。然而,在欧洲国家之间如何进行这种教育和培训缺乏协调,也没有适应指令2010/63/EU的建议。因此,FELASA和EFAT设立了一个工作组,其任务是为阿联工作人员的教育、培训和持续专业发展提出建议。工作组设立了五个不同的级别(阿联工作人员0-4级),确定了所需的能力和态度水平,并提出了达到每一级所需的教育条件。确定这些水平应有助于确保开展适当的教育和持续专业进修活动,并使雇主和阿联工作人员能够确定所达到的水平和职业阶段。此外,应为所有有关工作人员制订适当的能力评估和有效的持续专业进修计划。监管机构应通过制定能力评估标准并确保这些标准得到一致应用来支持这一点。此外,各机构应使阿联工作人员参与确定和发展关怀文化。动物福利机构应该参与并监督教育、培训和持续专业发展。这些建议将有助于协调和提高教育、培训和持续专业发展的质量,并为LAS员工提供更清晰的职业道路,有助于确保动物福利和科学的高标准。
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引用次数: 0
FELASA Working Group report: Capture and transport of live cephalopods – recommendations for scientific purposes FELASA工作组报告:活体头足类动物的捕获和运输-用于科学目的的建议
4区 农林科学 Q2 VETERINARY SCIENCES Pub Date : 2023-11-03 DOI: 10.1177/00236772231176347
Antonio V Sykes, Viola Galligioni, Juan Estefanell, Stuart Hetherington, Marco Brocca, Joao Correia, André Ferreira, Eleonora M. Pieroni, Graziano Fiorito
On 1 January 2013, research using cephalopod molluscs, from hatchlings to adults, became regulated within Directive 2010/63/EU. There are significant difficulties in captive breeding in the great majority of currently utilised species. Thus, scientific research relies upon the use of wild-caught animals. Furthermore, live cephalopods are shared and transported between different stakeholders and laboratories across Europe and other continents. Despite existing European and national legislation, codes, guidelines and reports from independent organisations, a set of recommendations specifically addressing the requirements for the capture and transport of animals belonging to this taxon are missing. In addition, although training and development of competence for all people involved in the supply chain are essential and aim to ensure that animals do not suffer from pain, distress or lasting harm, the requirements for those capturing and transporting wild cephalopods have not been considered. This Working Group reviewed the current literature to recognise scientific evidence and the best practice, and compiled a set of recommendations to provide guidance on the ‘techniques’ to be used for the capture and transport of live cephalopods for their use in scientific procedures. In addition, we propose to (a) develop standardised approaches able to assess recommended methods and objectively quantify the impact of these processes on animals’ health, welfare and stress response, and (b) design a training programme for people attaining the necessary competence for capture and transportation of live cephalopods, as required by Directive 2010/63/EU.
2013年1月1日,使用头足类软体动物(从幼体到成年)进行的研究受到指令2010/63/EU的监管。目前利用的绝大多数物种在圈养繁殖方面存在重大困难。因此,科学研究依赖于使用野生捕获的动物。此外,活体头足类动物在欧洲和其他大陆的不同利益相关者和实验室之间共享和运输。尽管现有的欧洲和国家立法、法规、指导方针和独立组织的报告,但一套专门针对属于该分类单元的动物的捕获和运输要求的建议仍然缺失。此外,尽管对参与供应链的所有人员进行能力培训和发展至关重要,其目的是确保动物不遭受痛苦、痛苦或持久伤害,但对捕获和运输野生头足类动物的要求尚未得到考虑。该工作组审查了现有文献,以确认科学证据和最佳做法,并编制了一套建议,为用于捕获和运输活头足类动物的“技术”提供指导,以供其用于科学程序。此外,我们建议(a)制定能够评估推荐方法的标准化方法,并客观量化这些过程对动物健康、福利和应激反应的影响,以及(b)根据指令2010/63/EU的要求,为获得捕获和运输活头足类动物所需能力的人员设计培训方案。
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引用次数: 0
FELASA recommendations for the rehoming of animals used for scientific and educational purposes. FELSA关于安置用于科学和教育目的的动物的建议。
IF 2.4 4区 农林科学 Q2 VETERINARY SCIENCES Pub Date : 2023-10-01 Epub Date: 2023-03-24 DOI: 10.1177/00236772231158863
Emilie Ecuer, Jackie Boxall, Annet L Louwerse, Lars F Mikkelsen, Christel Ph Moons, Mirjam Roth, Andrea M Spiri

Directive 2010/63/EU of the European Parliament and the Council of 22 September 2010 states that at the end of a procedure, the most appropriate decision on the future of an animal previously used or intended for use in scientific procedures should be taken on the basis of animal welfare and potential risks to the environment. Member States may allow animals to be rehomed provided the health of the animal allows it, there is no danger to public health, animal health or the environment and if appropriate measures have been taken to safeguard the wellbeing of the animal. In countries where rehoming is permitted, it is the responsibility of the Animal Welfare Body to advise on a rehoming scheme which must include appropriate socialization in order to help facilitate successful rehoming, avoid unnecessary distress to the animals and guarantee public safety. This paper reviews the EU legislation, existing guidance, current literature and best practice to define rehoming, sets out general considerations for rehoming laboratory animals including socialization and provides practical advice on the steps required in a rehoming scheme. For those species most frequently rehomed, more detailed species-specific sections are included.

欧洲议会和理事会2010年9月22日第2010/63/EU号指令规定,在程序结束时,应根据动物福利和对环境的潜在风险,对以前在科学程序中使用或打算使用的动物的未来做出最适当的决定。成员国可以允许动物重新安置,前提是动物的健康状况允许,对公共健康、动物健康或环境没有危险,并且已采取适当措施保障动物的健康。在允许安置的国家,动物福利机构有责任就安置计划提供建议,该计划必须包括适当的社会化,以帮助促进成功安置,避免对动物造成不必要的痛苦,并保障公共安全。本文回顾了欧盟立法、现有指南、当前文献和定义安置的最佳实践,阐述了安置实验动物的一般考虑因素,包括社会化,并就安置计划所需的步骤提供了实用建议。对于那些最经常被安置的物种,包括了更详细的特定物种章节。
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引用次数: 2
Evaluation of the effect of tramadol, paracetamol and metamizole on the severity of experimental colitis. 曲马多、对乙酰氨基酚和安乃近对实验性结肠炎严重程度影响的评估。
IF 2.4 4区 农林科学 Q2 VETERINARY SCIENCES Pub Date : 2023-10-01 Epub Date: 2023-03-24 DOI: 10.1177/00236772231163957
Marianne Spalinger, Marlene Schwarzfischer, Anna Niechcial, Kirstin Atrott, Andrea Laimbacher, Paulin Jirkof, Michael Scharl

Application of dextran sodium sulfate (DSS) is often used to induce experimental colitis. Current state of the art is to refrain from the use of analgesics due to their possible interaction with the model. However, the use of analgesics would be beneficial to reduce the overall constraint imposed on the animals. Here, we analyzed the effect of the analgesics Dafalgan (paracetamol), Tramal (tramadol) and Novalgin (metamizole) on DSS-induced colitis. To study the effect of those analgesics in colitis mouse models, acute and chronic colitis was induced in female C57BL6 mice by DSS administration in the drinking water. Analgesics were added to the drinking water on days four to seven (acute colitis) or on days six to nine of each DSS cycle (chronic colitis). Tramadol and paracetamol had minor effects on colitis severity. Tramadol reduced water uptake and activity levels slightly, while mice receiving paracetamol presented with a better overall appearance. Metamizole, however, significantly reduced water uptake, resulting in pronounced weight loss. In conclusion, our experiments show that tramadol and paracetamol are viable options for the use in DSS-induced colitis models. However, paracetamol seems to be slightly more favorable since it promoted the overall wellbeing of the animals upon DSS administration without interfering with typical readouts of colitis severity.

右旋糖酐硫酸钠(DSS)的应用通常用于诱导实验性结肠炎。目前的技术状态是避免使用止痛药,因为它们可能与模型相互作用。然而,使用止痛药将有利于减少对动物的总体限制。在这里,我们分析了止痛药Dafalgan(扑热息痛)、Tramal(曲马多)和Novalgin(安乃近)对DSS诱导的结肠炎的影响。为了研究这些镇痛剂在结肠炎小鼠模型中的作用,通过饮用水中DSS给药在雌性C57BL6小鼠中诱导急性和慢性结肠炎。在每个DSS周期的第4天至第7天(急性结肠炎)或第6天至第9天(慢性结肠炎)将镇痛剂添加到饮用水中。曲马多和扑热息痛对结肠炎的严重程度影响较小。曲马多略微降低了小鼠的水分摄取和活动水平,而接受扑热息痛治疗的小鼠整体外观更好。然而,异咪唑显著降低了吸水率,导致体重明显减轻。总之,我们的实验表明,曲马多和扑热息痛是用于DSS诱导的结肠炎模型的可行选择。然而,扑热息痛似乎更有利,因为它在DSS给药后促进了动物的整体健康,而不会干扰结肠炎严重程度的典型读数。
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引用次数: 0
Expression of Concern: "Advances in research on the biology of the desert rodent Gerbillus tarabuli: a review". 关注的表达:“沙漠啮齿类动物塔拉布力的生物学研究进展:综述”。
IF 2.4 4区 农林科学 Q2 VETERINARY SCIENCES Pub Date : 2023-10-01 Epub Date: 2023-07-20 DOI: 10.1177/00236772231187833
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引用次数: 0
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