Plant responses to nitrate, phosphate and sucrose form a complex molecular network crucial for terrestrial adaptation. However, the origins, functional diversity and evolvability of this network during plant terrestrialization remain scarcely understood. Here we compare the transcriptomic response to these nutrients in the bryophyte Marchantia polymorpha and the streptophyte alga Klebsormidium nitens. We show that the largely species-specific nutrient response pattern is driven by gene regulatory network (GRN) alterations. Intriguingly, while pathways governing the GRNs exhibit modest conservation, M. polymorpha GRNs exhibit more regulatory connections through the redeployment of ancient transcription factor CSD. In M. polymorpha, functional analyses reveal the involvement of pre-existing cytokinin machineries in downstream targets, orchestrating plastic morpho-physiological responses to nutrient status. Our findings implicate the genetic co-option events facilitating successful land plant establishment. The first land plant faced environmental challenges during terrestrial colonization. This study shows how the co-option of gene regulatory networks contributes to nutrient responses, facilitating the terrestrial adaptation of ancestral land plants.
植物对硝酸盐、磷酸盐和蔗糖的反应形成了一个复杂的分子网络，对陆地适应至关重要。然而，人们对这一网络在植物陆地化过程中的起源、功能多样性和可演化性仍然知之甚少。在这里，我们比较了裸子植物 Marchantia polymorpha 和链格藻 Klebsormidium nitens 对这些营养物质的转录组反应。我们发现，基因调控网络（GRN）的改变在很大程度上驱动了物种特异性的营养响应模式。耐人寻味的是，虽然调控基因调控网络的途径表现出适度的保守性，但 M. polymorpha 的基因调控网络通过重新部署古老的转录因子 CSD 而表现出更多的调控联系。在 M. polymorpha 中，功能分析揭示了已有的细胞分裂素机制参与下游目标，协调对营养状况的可塑性形态生理反应。我们的研究结果揭示了促进陆生植物成功建立的基因共通事件。
{"title":"Co-option of plant gene regulatory network in nutrient responses during terrestrialization","authors":"Yating Dong, Shalini Krishnamoorthi, Grace Zi Hao Tan, Zheng Yong Poh, Daisuke Urano","doi":"10.1038/s41477-024-01851-4","DOIUrl":"10.1038/s41477-024-01851-4","url":null,"abstract":"Plant responses to nitrate, phosphate and sucrose form a complex molecular network crucial for terrestrial adaptation. However, the origins, functional diversity and evolvability of this network during plant terrestrialization remain scarcely understood. Here we compare the transcriptomic response to these nutrients in the bryophyte Marchantia polymorpha and the streptophyte alga Klebsormidium nitens. We show that the largely species-specific nutrient response pattern is driven by gene regulatory network (GRN) alterations. Intriguingly, while pathways governing the GRNs exhibit modest conservation, M. polymorpha GRNs exhibit more regulatory connections through the redeployment of ancient transcription factor CSD. In M. polymorpha, functional analyses reveal the involvement of pre-existing cytokinin machineries in downstream targets, orchestrating plastic morpho-physiological responses to nutrient status. Our findings implicate the genetic co-option events facilitating successful land plant establishment. The first land plant faced environmental challenges during terrestrial colonization. This study shows how the co-option of gene regulatory networks contributes to nutrient responses, facilitating the terrestrial adaptation of ancestral land plants.","PeriodicalId":18904,"journal":{"name":"Nature Plants","volume":"10 12","pages":"1955-1968"},"PeriodicalIF":15.8,"publicationDate":"2024-11-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142712794","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-11-25DOI: 10.1038/s41477-024-01830-9
Susanne S. Renner
The most widespread chromosomal sex determination systems in plants and animals with separate sexes are the XY system, in which males are heterogametic, and the ZW system, in which females are heterogametic. Figuring out which species has which system has become easier with fully phased, chromosome-scale genome assemblies. A new study now provides such a genome for Amborella trichopoda, with its Z and W sex chromosomes nearly completely assembled.
在有两性之分的动植物中，最普遍的染色体性别决定系统是雄性异配的 XY 系统和雌性异配的 ZW 系统。有了全相位、染色体尺度的基因组组装，找出哪个物种具有哪个系统就变得容易多了。现在，一项新的研究为 Amborella trichopoda 提供了这样一个基因组，其 Z 和 W 性染色体几乎完全组装完毕。
{"title":"Unlocking Amborella’s ZW sex chromosome system","authors":"Susanne S. Renner","doi":"10.1038/s41477-024-01830-9","DOIUrl":"10.1038/s41477-024-01830-9","url":null,"abstract":"The most widespread chromosomal sex determination systems in plants and animals with separate sexes are the XY system, in which males are heterogametic, and the ZW system, in which females are heterogametic. Figuring out which species has which system has become easier with fully phased, chromosome-scale genome assemblies. A new study now provides such a genome for Amborella trichopoda, with its Z and W sex chromosomes nearly completely assembled.","PeriodicalId":18904,"journal":{"name":"Nature Plants","volume":"10 12","pages":"1854-1856"},"PeriodicalIF":15.8,"publicationDate":"2024-11-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142697071","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-11-25DOI: 10.1038/s41477-024-01858-x
Sarah B. Carey, Laramie Aközbek, John T. Lovell, Jerry Jenkins, Adam L. Healey, Shengqiang Shu, Paul Grabowski, Alan Yocca, Ada Stewart, Teresa Jones, Kerrie Barry, Shanmugam Rajasekar, Jayson Talag, Charlie Scutt, Porter P. Lowry II, Jérôme Munzinger, Eric B. Knox, Douglas E. Soltis, Pamela S. Soltis, Jane Grimwood, Jeremy Schmutz, James Leebens-Mack, Alex Harkess
Sex chromosomes have evolved hundreds of times across the flowering plant tree of life; their recent origins in some members of this clade can shed light on the early consequences of suppressed recombination, a crucial step in sex chromosome evolution. Amborella trichopoda, the sole species of a lineage that is sister to all other extant flowering plants, is dioecious with a young ZW sex determination system. Here we present a haplotype-resolved genome assembly, including highly contiguous assemblies of the Z and W chromosomes. We identify a ~3-megabase sex-determination region (SDR) captured in two strata that includes a ~300-kilobase inversion that is enriched with repetitive sequences and contains a homologue of the Arabidopsis METHYLTHIOADENOSINE NUCLEOSIDASE (MTN1-2) genes, which are known to be involved in fertility. However, the remainder of the SDR does not show patterns typically found in non-recombining SDRs, such as repeat accumulation and gene loss. These findings are consistent with the hypothesis that dioecy is derived in Amborella and the sex chromosome pair has not significantly degenerated. The haplotype-resolved genome in Amborella trichopoda addresses outstanding questions on the structure and gene content of the recently evolved ZW sex chromosomes.
性染色体在有花植物生命树上已经进化了数百次；它们最近在该支系一些成员中的起源可以揭示抑制重组的早期后果，这是性染色体进化的一个关键步骤。Amborella trichopoda是与所有其他现存有花植物为姐妹系的唯一物种，雌雄异株，具有年轻的ZW性别决定系统。在这里，我们展示了一个单体型解析的基因组组装，包括高度连续的 Z 染色体和 W 染色体的组装。我们发现了一个约 3 兆碱基的性别决定区（SDR），该区包括一个约 300 千碱基的反转，富含重复序列，并含有拟南芥甲基硫代腺苷核苷酸酶（MTN1-2）基因的同源物，而这些基因已知与生育力有关。然而，SDR 的其余部分并没有表现出非重组 SDR 的典型模式，如重复积累和基因缺失。这些发现与雌雄异体的假说一致，即在 Amborella 中雌雄异体是派生的，性染色体对没有明显退化。
{"title":"ZW sex chromosome structure in Amborella trichopoda","authors":"Sarah B. Carey, Laramie Aközbek, John T. Lovell, Jerry Jenkins, Adam L. Healey, Shengqiang Shu, Paul Grabowski, Alan Yocca, Ada Stewart, Teresa Jones, Kerrie Barry, Shanmugam Rajasekar, Jayson Talag, Charlie Scutt, Porter P. Lowry II, Jérôme Munzinger, Eric B. Knox, Douglas E. Soltis, Pamela S. Soltis, Jane Grimwood, Jeremy Schmutz, James Leebens-Mack, Alex Harkess","doi":"10.1038/s41477-024-01858-x","DOIUrl":"10.1038/s41477-024-01858-x","url":null,"abstract":"Sex chromosomes have evolved hundreds of times across the flowering plant tree of life; their recent origins in some members of this clade can shed light on the early consequences of suppressed recombination, a crucial step in sex chromosome evolution. Amborella trichopoda, the sole species of a lineage that is sister to all other extant flowering plants, is dioecious with a young ZW sex determination system. Here we present a haplotype-resolved genome assembly, including highly contiguous assemblies of the Z and W chromosomes. We identify a ~3-megabase sex-determination region (SDR) captured in two strata that includes a ~300-kilobase inversion that is enriched with repetitive sequences and contains a homologue of the Arabidopsis METHYLTHIOADENOSINE NUCLEOSIDASE (MTN1-2) genes, which are known to be involved in fertility. However, the remainder of the SDR does not show patterns typically found in non-recombining SDRs, such as repeat accumulation and gene loss. These findings are consistent with the hypothesis that dioecy is derived in Amborella and the sex chromosome pair has not significantly degenerated. The haplotype-resolved genome in Amborella trichopoda addresses outstanding questions on the structure and gene content of the recently evolved ZW sex chromosomes.","PeriodicalId":18904,"journal":{"name":"Nature Plants","volume":"10 12","pages":"1944-1954"},"PeriodicalIF":15.8,"publicationDate":"2024-11-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.nature.com/articles/s41477-024-01858-x.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142697073","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-11-22DOI: 10.1038/s41477-024-01865-y
Ziwei Lin, Ying Guo, Ruiyuan Zhang, Yiming Li, Yue Wu, Jen Sheen, Kun-hsiang Liu
Abscisic acid (ABA) regulates plant stress adaptation, growth and reproduction. Despite extensive ABA–Ca2+ signalling links, imaging ABA-induced increases in Ca2+ concentration has been challenging, except in guard cells. Here we visualize ABA-triggered [Ca2+] dynamics in diverse organs and cell types of Arabidopsis thaliana using a genetically encoded Ca2+ ratiometric sensor with a low-nanomolar Ca2+-binding affinity and a large dynamic range. The subcellular-targeted Ca2+ ratiometric sensor reveals time-resolved and unique spatiotemporal Ca2+ signatures from the initial plasma-membrane nanodomain, to cytosol, to nuclear oscillation. Via receptors and sucrose-non-fermenting1-related protein kinases (SnRK2.2/2.3/2.6), ABA activates low-nanomolar Ca2+ transient and Ca2+-sensor protein kinase (CPK10/30/32) signalling in the root cap cycle from stem cells to cell detachment. Surprisingly, unlike the prevailing NaCl-stimulated micromolar Ca2+ spike, salt stress induces a low-nanomolar Ca2+ transient through ABA signalling, repressing key transcription factors that dictate cell fate and enzymes that are crucial to root cap maturation and slough. Our findings uncover ABA–Ca2+–CPK signalling that modulates root cap cycle plasticity in adaptation to adverse environments. This study reveals ABA-triggered low-nanomolar Ca2+ dynamics in diverse plant organs and cell types using an ultrasensitive Ca2+ biosensor. Spatiotemporal Ca2+ dynamics modulate the root cap cycle in adaptation to stress through ABA–Ca2+–CPK signalling.
脱落酸（ABA）调节植物的胁迫适应、生长和繁殖。尽管 ABA 与 Ca2+ 信号有广泛的联系，但除了在保卫细胞中，对 ABA 诱导的 Ca2+ 浓度增加进行成像一直是一项挑战。在这里，我们利用一种基因编码的 Ca2+ 比率传感器（具有低纳摩尔 Ca2+ 结合亲和力和较大的动态范围）对拟南芥不同器官和细胞类型中 ABA 触发的[Ca2+]动态进行了可视化。这种亚细胞靶向 Ca2+ 比率测定传感器揭示了从最初的质膜纳米域到细胞质再到核振荡的时间分辨和独特的时空 Ca2+ 特征。通过受体和蔗糖-非发酵1相关蛋白激酶（SnRK2.2/2.3/2.6），ABA激活了从干细胞到细胞脱落的根帽周期中的低纳摩尔Ca2+瞬时和Ca2+感应蛋白激酶（CPK10/30/32）信号。令人惊讶的是，与普遍的 NaCl 刺激微摩尔 Ca2+ 峰值不同，盐胁迫通过 ABA 信号诱导低纳摩尔 Ca2+ 瞬态，抑制决定细胞命运的关键转录因子以及对根帽成熟和脱落至关重要的酶。我们的研究结果揭示了 ABA-Ca2+-CPK 信号在适应不利环境过程中调节根帽周期可塑性的作用。
{"title":"ABA-activated low-nanomolar Ca2+–CPK signalling controls root cap cycle plasticity and stress adaptation","authors":"Ziwei Lin, Ying Guo, Ruiyuan Zhang, Yiming Li, Yue Wu, Jen Sheen, Kun-hsiang Liu","doi":"10.1038/s41477-024-01865-y","DOIUrl":"10.1038/s41477-024-01865-y","url":null,"abstract":"Abscisic acid (ABA) regulates plant stress adaptation, growth and reproduction. Despite extensive ABA–Ca2+ signalling links, imaging ABA-induced increases in Ca2+ concentration has been challenging, except in guard cells. Here we visualize ABA-triggered [Ca2+] dynamics in diverse organs and cell types of Arabidopsis thaliana using a genetically encoded Ca2+ ratiometric sensor with a low-nanomolar Ca2+-binding affinity and a large dynamic range. The subcellular-targeted Ca2+ ratiometric sensor reveals time-resolved and unique spatiotemporal Ca2+ signatures from the initial plasma-membrane nanodomain, to cytosol, to nuclear oscillation. Via receptors and sucrose-non-fermenting1-related protein kinases (SnRK2.2/2.3/2.6), ABA activates low-nanomolar Ca2+ transient and Ca2+-sensor protein kinase (CPK10/30/32) signalling in the root cap cycle from stem cells to cell detachment. Surprisingly, unlike the prevailing NaCl-stimulated micromolar Ca2+ spike, salt stress induces a low-nanomolar Ca2+ transient through ABA signalling, repressing key transcription factors that dictate cell fate and enzymes that are crucial to root cap maturation and slough. Our findings uncover ABA–Ca2+–CPK signalling that modulates root cap cycle plasticity in adaptation to adverse environments. This study reveals ABA-triggered low-nanomolar Ca2+ dynamics in diverse plant organs and cell types using an ultrasensitive Ca2+ biosensor. Spatiotemporal Ca2+ dynamics modulate the root cap cycle in adaptation to stress through ABA–Ca2+–CPK signalling.","PeriodicalId":18904,"journal":{"name":"Nature Plants","volume":"11 1","pages":"90-104"},"PeriodicalIF":15.8,"publicationDate":"2024-11-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142684157","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-11-22DOI: 10.1038/s41477-024-01872-z
The complete genome assembly of the model plant Nicotiana benthamiana reveals the genetic and epigenetic landscape of its centromeres, which have evolved via satellite expansion, retrotransposon enrichment, and mitochondrial DNA integration. These insights enhance knowledge of polyploid centromere diversity and evolution in Solanaceae species.
模式植物烟草（Nicotiana benthamiana）的完整基因组组装揭示了其中心粒的遗传和表观遗传景观，这些中心粒是通过卫星扩展、反转座子富集和线粒体 DNA 整合进化而来的。这些发现增进了对茄科植物多倍体中心粒多样性和进化的了解。
{"title":"Unravelling the centromere landscape and evolution in the model plant Nicotiana benthamiana","authors":"","doi":"10.1038/s41477-024-01872-z","DOIUrl":"10.1038/s41477-024-01872-z","url":null,"abstract":"The complete genome assembly of the model plant Nicotiana benthamiana reveals the genetic and epigenetic landscape of its centromeres, which have evolved via satellite expansion, retrotransposon enrichment, and mitochondrial DNA integration. These insights enhance knowledge of polyploid centromere diversity and evolution in Solanaceae species.","PeriodicalId":18904,"journal":{"name":"Nature Plants","volume":"10 12","pages":"1863-1864"},"PeriodicalIF":15.8,"publicationDate":"2024-11-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142684216","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-11-21DOI: 10.1038/s41477-024-01860-3
Climate warming is accelerating successional processes, leading to late-successional species (fir) quickly outcompeting pioneer species (birch) at Himalayan treelines. This finding offers insights into the composition and interactions of future forest species, as well as their influences on ecosystem services.
{"title":"Climate warming speeds up species succession in Himalayan treelines","authors":"","doi":"10.1038/s41477-024-01860-3","DOIUrl":"10.1038/s41477-024-01860-3","url":null,"abstract":"Climate warming is accelerating successional processes, leading to late-successional species (fir) quickly outcompeting pioneer species (birch) at Himalayan treelines. This finding offers insights into the composition and interactions of future forest species, as well as their influences on ecosystem services.","PeriodicalId":18904,"journal":{"name":"Nature Plants","volume":"10 12","pages":"1859-1860"},"PeriodicalIF":15.8,"publicationDate":"2024-11-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142678221","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-11-18DOI: 10.1038/s41477-024-01870-1
The functions of many genes in model plants are unknown. Traditional methods to infer function are showing their limitations, but other approaches are emerging.
模式植物中许多基因的功能尚不清楚。推断功能的传统方法已显示出其局限性，但其他方法正在出现。
{"title":"Alternate routes to gene functions","authors":"","doi":"10.1038/s41477-024-01870-1","DOIUrl":"10.1038/s41477-024-01870-1","url":null,"abstract":"The functions of many genes in model plants are unknown. Traditional methods to infer function are showing their limitations, but other approaches are emerging.","PeriodicalId":18904,"journal":{"name":"Nature Plants","volume":"10 11","pages":"1605-1606"},"PeriodicalIF":15.8,"publicationDate":"2024-11-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.nature.com/articles/s41477-024-01870-1.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142668247","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-11-18DOI: 10.1038/s41477-024-01855-0
Shalik Ram Sigdel, Xiangyu Zheng, Flurin Babst, J. Julio Camarero, Shan Gao, Xiaoxia Li, Xiaoming Lu, Jayram Pandey, Binod Dawadi, Jian Sun, Haifeng Zhu, Tao Wang, Eryuan Liang, Josep Peñuelas
Understanding how climate change influences succession is fundamental for predicting future forest composition. Warming is expected to accelerate species succession at their cold thermal ranges, such as alpine treelines. Here we examined how interactions and successional strategies of the early-successional birch (Betula utilis) and the late-successional fir (Abies spectabilis) affected treeline dynamics by combining plot data with an individual-based treeline model at treelines in the central Himalayas. Fir showed increasing recruitment and a higher upslope shift rate (0.11 ± 0.02 m yr−1) compared with birch (0.06 ± 0.03 m yr−1) over the past 200 years. Spatial analyses indicate strong interspecies competition when trees were young. Model outputs from various climatic scenarios indicate that fir will probably accelerate its upslope movement with warming, while birch recruitment will decline drastically, forming stable or even retreating treelines. Our findings point to accelerating successional dynamics with late-successional species rapidly outcompeting pioneer species, offering insight into future forest succession and its influences on ecosystem services. Climate warming is accelerating successional dynamics, with late-successional species rapidly outcompeting pioneer species at Himalayan treeline ecotones, offering insight into future forest succession and its influences on ecosystem services.
{"title":"Accelerated succession in Himalayan alpine treelines under climatic warming","authors":"Shalik Ram Sigdel, Xiangyu Zheng, Flurin Babst, J. Julio Camarero, Shan Gao, Xiaoxia Li, Xiaoming Lu, Jayram Pandey, Binod Dawadi, Jian Sun, Haifeng Zhu, Tao Wang, Eryuan Liang, Josep Peñuelas","doi":"10.1038/s41477-024-01855-0","DOIUrl":"10.1038/s41477-024-01855-0","url":null,"abstract":"Understanding how climate change influences succession is fundamental for predicting future forest composition. Warming is expected to accelerate species succession at their cold thermal ranges, such as alpine treelines. Here we examined how interactions and successional strategies of the early-successional birch (Betula utilis) and the late-successional fir (Abies spectabilis) affected treeline dynamics by combining plot data with an individual-based treeline model at treelines in the central Himalayas. Fir showed increasing recruitment and a higher upslope shift rate (0.11 ± 0.02 m yr−1) compared with birch (0.06 ± 0.03 m yr−1) over the past 200 years. Spatial analyses indicate strong interspecies competition when trees were young. Model outputs from various climatic scenarios indicate that fir will probably accelerate its upslope movement with warming, while birch recruitment will decline drastically, forming stable or even retreating treelines. Our findings point to accelerating successional dynamics with late-successional species rapidly outcompeting pioneer species, offering insight into future forest succession and its influences on ecosystem services. Climate warming is accelerating successional dynamics, with late-successional species rapidly outcompeting pioneer species at Himalayan treeline ecotones, offering insight into future forest succession and its influences on ecosystem services.","PeriodicalId":18904,"journal":{"name":"Nature Plants","volume":"10 12","pages":"1909-1918"},"PeriodicalIF":15.8,"publicationDate":"2024-11-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142665499","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-11-15DOI: 10.1038/s41477-024-01857-y
Jean-David Rochaix
The pyrenoid contains internal membrane structures that are required for efficient carbon fixation. The two proteins SAGA1 and MITH1 are necessary for the biogenesis of these membranes and the delivery of bicarbonate to the pyrenoid matrix.
{"title":"New light on pyrenoid membrane tubules","authors":"Jean-David Rochaix","doi":"10.1038/s41477-024-01857-y","DOIUrl":"10.1038/s41477-024-01857-y","url":null,"abstract":"The pyrenoid contains internal membrane structures that are required for efficient carbon fixation. The two proteins SAGA1 and MITH1 are necessary for the biogenesis of these membranes and the delivery of bicarbonate to the pyrenoid matrix.","PeriodicalId":18904,"journal":{"name":"Nature Plants","volume":"10 12","pages":"1852-1853"},"PeriodicalIF":15.8,"publicationDate":"2024-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142637273","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-11-15DOI: 10.1038/s41477-024-01847-0
Jessica H. Hennacy, Nicky Atkinson, Angelo Kayser-Browne, Sabrina L. Ergun, Eric Franklin, Lianyong Wang, Simona Eicke, Yana Kazachkova, Moshe Kafri, Friedrich Fauser, Josep Vilarrasa-Blasi, Robert E. Jinkerson, Samuel C. Zeeman, Alistair J. McCormick, Martin C. Jonikas
Approximately one-third of global CO2 assimilation is performed by the pyrenoid, a liquid-like organelle found in most algae and some plants. Specialized pyrenoid-traversing membranes are hypothesized to drive CO2 assimilation in the pyrenoid by delivering concentrated CO2, but how these membranes are made to traverse the pyrenoid matrix remains unknown. Here we show that proteins SAGA1 and MITH1 cause membranes to traverse the pyrenoid matrix in the model alga Chlamydomonas reinhardtii. Mutants deficient in SAGA1 or MITH1 lack matrix-traversing membranes and exhibit growth defects under CO2-limiting conditions. Expression of SAGA1 and MITH1 together in a heterologous system, the model plant Arabidopsis thaliana, produces matrix-traversing membranes. Both proteins localize to matrix-traversing membranes. SAGA1 binds to the major matrix component, Rubisco, and is necessary to initiate matrix-traversing membranes. MITH1 binds to SAGA1 and is necessary for extension of membranes through the matrix. Our data suggest that SAGA1 and MITH1 cause membranes to traverse the matrix by creating an adhesive interaction between the membrane and matrix. Our study identifies and characterizes key factors in the biogenesis of pyrenoid matrix-traversing membranes, demonstrates the importance of these membranes to pyrenoid function and marks a key milestone toward pyrenoid engineering into crops for improving yields. Two algal proteins, MITH1 and SAGA1, play key roles in formation of membranes that deliver CO2 to the pyrenoid, a CO2-concentrating organelle. Their discovery marks a key milestone towards engineering a pyrenoid into land plants for improved yields.
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