Pub Date : 2024-06-17DOI: 10.1038/s41477-024-01701-3
Georg Oberhofer, Michelle L. Johnson, Tobin Ivy, Igor Antoshechkin, Bruce A. Hay
Gene drive elements promote the spread of linked traits and can be used to change the composition or fate of wild populations. Cleave and Rescue (ClvR) drive elements sit at a fixed chromosomal position and include a DNA sequence-modifying enzyme such as Cas9/gRNAs that disrupts endogenous versions of an essential gene and a recoded version of the essential gene resistant to cleavage. ClvR spreads by creating conditions in which those lacking ClvR die because they lack functional versions of the essential gene. Here we demonstrate the essential features of the ClvR gene drive in the plant Arabidopsis thaliana through killing of gametes that fail to inherit a ClvR that targets the essential gene YKT61. Resistant alleles, which can slow or prevent drive, were not observed. Modelling shows plant ClvRs are robust to certain failure modes and can be used to rapidly drive population modification or suppression. Possible applications are discussed. Gene drive elements spread linked traits and can be used to change the composition or fate of populations. Here Oberhofer and colleagues engineer gene drive in the plant Arabidopsis thaliana. Applications include genetic biocontrol and conservation.
{"title":"Cleave and Rescue gamete killers create conditions for gene drive in plants","authors":"Georg Oberhofer, Michelle L. Johnson, Tobin Ivy, Igor Antoshechkin, Bruce A. Hay","doi":"10.1038/s41477-024-01701-3","DOIUrl":"10.1038/s41477-024-01701-3","url":null,"abstract":"Gene drive elements promote the spread of linked traits and can be used to change the composition or fate of wild populations. Cleave and Rescue (ClvR) drive elements sit at a fixed chromosomal position and include a DNA sequence-modifying enzyme such as Cas9/gRNAs that disrupts endogenous versions of an essential gene and a recoded version of the essential gene resistant to cleavage. ClvR spreads by creating conditions in which those lacking ClvR die because they lack functional versions of the essential gene. Here we demonstrate the essential features of the ClvR gene drive in the plant Arabidopsis thaliana through killing of gametes that fail to inherit a ClvR that targets the essential gene YKT61. Resistant alleles, which can slow or prevent drive, were not observed. Modelling shows plant ClvRs are robust to certain failure modes and can be used to rapidly drive population modification or suppression. Possible applications are discussed. Gene drive elements spread linked traits and can be used to change the composition or fate of populations. Here Oberhofer and colleagues engineer gene drive in the plant Arabidopsis thaliana. Applications include genetic biocontrol and conservation.","PeriodicalId":18904,"journal":{"name":"Nature Plants","volume":null,"pages":null},"PeriodicalIF":15.8,"publicationDate":"2024-06-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141333758","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-06-17DOI: 10.1038/s41477-024-01723-x
Paul Neve, Luke Barrett
Two groups of scientists independently engineer gene drives in Arabidopsis thaliana, demonstrating the possibility for spreading fitness-reducing genetic modifications through wild populations of plants for population suppression.
{"title":"Driving evolution in wild plants","authors":"Paul Neve, Luke Barrett","doi":"10.1038/s41477-024-01723-x","DOIUrl":"10.1038/s41477-024-01723-x","url":null,"abstract":"Two groups of scientists independently engineer gene drives in Arabidopsis thaliana, demonstrating the possibility for spreading fitness-reducing genetic modifications through wild populations of plants for population suppression.","PeriodicalId":18904,"journal":{"name":"Nature Plants","volume":null,"pages":null},"PeriodicalIF":15.8,"publicationDate":"2024-06-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141333540","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-06-17DOI: 10.1038/s41477-024-01692-1
Yang Liu, Bingke Jiao, Jackson Champer, Wenfeng Qian
Synthetic gene drives, inspired by natural selfish genetic elements and transmitted to progeny at super-Mendelian (>50%) frequencies, present transformative potential for disseminating traits that benefit humans throughout wild populations, even facing potential fitness costs. Here we constructed a gene drive system in plants called CRISPR-Assisted Inheritance utilizing NPG1 (CAIN), which uses a toxin–antidote mechanism in the male germline to override Mendelian inheritance. Specifically, a guide RNA–Cas9 cassette targets the essential No Pollen Germination 1 (NPG1) gene, serving as the toxin to block pollen germination. A recoded, CRISPR-resistant copy of NPG1 serves as the antidote, providing rescue only in pollen cells that carry the drive. To limit potential consequences of inadvertent release, we used self-pollinating Arabidopsis thaliana as a model. The drive demonstrated a robust 88–99% transmission rate over two successive generations, producing minimal resistance alleles that are unlikely to inhibit drive spread. Our study provides a strong basis for rapid genetic modification or suppression of outcrossing plant populations. Researchers have developed a synthetic gene drive in Arabidopsis, demonstrating a proof of concept for biasing inheritance that could enable rapid genetic modifications or population suppression in wild plants.
{"title":"Overriding Mendelian inheritance in Arabidopsis with a CRISPR toxin–antidote gene drive that impairs pollen germination","authors":"Yang Liu, Bingke Jiao, Jackson Champer, Wenfeng Qian","doi":"10.1038/s41477-024-01692-1","DOIUrl":"10.1038/s41477-024-01692-1","url":null,"abstract":"Synthetic gene drives, inspired by natural selfish genetic elements and transmitted to progeny at super-Mendelian (>50%) frequencies, present transformative potential for disseminating traits that benefit humans throughout wild populations, even facing potential fitness costs. Here we constructed a gene drive system in plants called CRISPR-Assisted Inheritance utilizing NPG1 (CAIN), which uses a toxin–antidote mechanism in the male germline to override Mendelian inheritance. Specifically, a guide RNA–Cas9 cassette targets the essential No Pollen Germination 1 (NPG1) gene, serving as the toxin to block pollen germination. A recoded, CRISPR-resistant copy of NPG1 serves as the antidote, providing rescue only in pollen cells that carry the drive. To limit potential consequences of inadvertent release, we used self-pollinating Arabidopsis thaliana as a model. The drive demonstrated a robust 88–99% transmission rate over two successive generations, producing minimal resistance alleles that are unlikely to inhibit drive spread. Our study provides a strong basis for rapid genetic modification or suppression of outcrossing plant populations. Researchers have developed a synthetic gene drive in Arabidopsis, demonstrating a proof of concept for biasing inheritance that could enable rapid genetic modifications or population suppression in wild plants.","PeriodicalId":18904,"journal":{"name":"Nature Plants","volume":null,"pages":null},"PeriodicalIF":15.8,"publicationDate":"2024-06-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141333728","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-06-10DOI: 10.1038/s41477-024-01737-5
Andreas Naschberger, Mariia Fadeeva, Daniel Klaiman, Anna Borovikova-Sheinker, Ido Caspy, Nathan Nelson, Alexey Amunts
{"title":"Author Correction: Structure of plant photosystem I in a native assembly state defines PsaF as a regulatory checkpoint","authors":"Andreas Naschberger, Mariia Fadeeva, Daniel Klaiman, Anna Borovikova-Sheinker, Ido Caspy, Nathan Nelson, Alexey Amunts","doi":"10.1038/s41477-024-01737-5","DOIUrl":"10.1038/s41477-024-01737-5","url":null,"abstract":"","PeriodicalId":18904,"journal":{"name":"Nature Plants","volume":null,"pages":null},"PeriodicalIF":15.8,"publicationDate":"2024-06-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11208144/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141301150","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-06-07DOI: 10.1038/s41477-024-01727-7
Hwan-Ching Tai, Cheng-Si Tsao, Jer-Horng Lin
{"title":"Reply to: Critical comment on the assumptions leading to 24-chain microfibrils in wood","authors":"Hwan-Ching Tai, Cheng-Si Tsao, Jer-Horng Lin","doi":"10.1038/s41477-024-01727-7","DOIUrl":"10.1038/s41477-024-01727-7","url":null,"abstract":"","PeriodicalId":18904,"journal":{"name":"Nature Plants","volume":null,"pages":null},"PeriodicalIF":15.8,"publicationDate":"2024-06-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141287226","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-06-06DOI: 10.1038/s41477-024-01722-y
Hybrid seed production is a labour-intensive manual process that limits fully mechanized hybrid rice breeding. We identify GSE3 as a gene that regulates grain size and demonstrate that fully mechanized hybrid seed production and increased seed number can be achieved using small-grain alleles of GSE3 in male sterile lines.
{"title":"Genetic editing of grain size genes enables fully mechanized hybrid rice breeding","authors":"","doi":"10.1038/s41477-024-01722-y","DOIUrl":"10.1038/s41477-024-01722-y","url":null,"abstract":"Hybrid seed production is a labour-intensive manual process that limits fully mechanized hybrid rice breeding. We identify GSE3 as a gene that regulates grain size and demonstrate that fully mechanized hybrid seed production and increased seed number can be achieved using small-grain alleles of GSE3 in male sterile lines.","PeriodicalId":18904,"journal":{"name":"Nature Plants","volume":null,"pages":null},"PeriodicalIF":15.8,"publicationDate":"2024-06-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141264870","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-06-05DOI: 10.1038/s41477-024-01732-w
Nemanja Vukašinović, Yaowei Wang, Isabelle Vanhoutte, Matyáš Fendrych, Boyu Guo, Miroslav Kvasnica, Petra Jiroutová, Jana Oklestkova, Miroslav Strnad, Eugenia Russinova
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