Nutrition & Metabolism最新文献

Background: Persistent inflammation plays a crucial role in the pathogenesis of metabolic dysfunction-associated steatotic liver disease (MASLD). We aimed to scrutinize the associations of diet-induced inflammation with the improvement or worsening of hepatic steatosis and fibrosis in MASLD.

Methods: This longitudinal study involved 2,537 participants from the Ravanser Non-Communicable Disease (RaNCD) cohort (2015-2023). Dietary intake was assessed using the 118-item food frequency questionnaire (FFQ), and diet-induced inflammation was determined using the dietary inflammatory index (DII). The AST to platelet ratio index (APRI) and Fibrosis-4 (FIB-4) were used as confirmed predictive indicators for hepatic fibrosis and the hepatic steatosis index (HSI) was used for hepatic steatosis.

Results: Adherence to an inflammatory diet independently increases the risk of worsening hepatic steatosis (RR:1.39; 95%CI: 1.02-1.93; P-value: 0.04) and reduces the risk of improving hepatic steatosis (RR: 66; 95% CI: 0.48-0.98; P-value: 0.01) compared to an anti-inflammatory diet. The DII scores did not show any connection to hepatic fibrosis, as determined by FIB-4 (β: - 1.08; 95%CI: - 2.43 to 0.27; P-value: 0.12) and APRI (β: 0.22; 95%CI: - 1.51 to 1.95; P-value: 0.80).

Conclusions: These results underscore the importance of dietary composition in managing hepatic steatosis and highlight the need for further research to explore the mechanisms underlying these associations.

Background: Constipation is one of the most common gastrointestinal complaints during pregnancy. Consuming fruits and vegetables is often the first line of treatment due to their fiber content. Therefore, the purpose of the present study was to determine the effect of combined fig-walnut syrup on functional constipation (FC) and quality of life (QoL) in pregnant women.

Methods: In this double-blind, randomized controlled clinical trial, 90 pregnant women with FC were randomly assigned to receive combined fig-walnut syrup, fig syrup, or placebo (n = 30 in each group) using block randomization. Participants received 15 ml of syrup once daily at night, half an hour before bedtime, for 14 days and were followed up for 2 weeks after the end of the intervention. The FC, quality of life-gravidity (QOL-GRAV), and food frequency questionnaires (FFQ) were used to collect data. The questionnaires were completed once before the intervention and then the FC questionnaire was completed once a week for four weeks and the QoL questionnaire was completed at the end of the fourth week. To compare the outcomes among the study groups, one-way AONOVA, ANCOVA, Mann- Whitney U test, and Kruskal- Wallis test were used.

Results: Following the intervention, although there was no statistically significant difference in the number of defecations between the combined fig-walnut syrup group and the fig syrup group (P > 0.05) at every four weeks, there was a statistically significant difference compared to the placebo (P < 0.05). Combined fig-walnut syrup (adjusted mean difference (AMD): -3.4; 95% confidence interval: -0.7 to -6.1; P = 0.008) and fig syrup (AMD: -5.8; 95% CI: -3.1 to -8.6; P < 0.001) improved QoL compared to the placebo group and there was no statistically significant difference between the combined fig-walnut and fig syrup (AMD: 2.4; 95% CI: 5.2 to -0.3; P = 0.104).

Conclusion: The consumption of fig-walnut syrup and fig syrup may help improve constipation symptoms and enhance QoL during pregnancy. Further studies are needed to reach a conclusive determination.

Trial registration: Iranian Registry of Clinical Trials (IRCT): IRCT2012071801032N79. Date of registration: 07.12.2023.

Background: Serum vitamin D deficiency is intricately linked to metabolic disorders, however, evidence on its association with continuous metabolic risk in children and adolescents remains insufficient. This study aims to elucidate the relationship between serum vitamin D levels and continuous metabolic risk.

Methods: The cross-sectional analysis involved 4490 participants aged 6 ~ 18, and the longitudinal investigation included 1398 individuals aged 6 ~ 12 years. Serum 25(OH)D concentrations were quantified using liquid chromatography-mass spectrometry. Continuous Metabolic syndrome risk score (CMSRS), incorporating waist, blood pressure, blood lipid levels, and glucose metabolism as four components, utilizes age- and gender-specific Z scores to evaluate metabolic risk. Restricted cubic splines (RCS) were used to visualize dose-response relationships and generalized linear models (GLM) were used to estimate potential associations. Mediation analysis was used to evaluate the mediating role of levels of Neutrophil-to-lymphocyte ratio (NLR).

Results: The RCS indicated a negative linear association between serum 25(OH)D levels and CMSRS (P-overall = 0.0066, P-nonlinear = 0.1393). GLM revealed that compared to Q1, with the quartiles of serum 25(OH)D concentrations increase, the β value ranged from 0.028 (95% CI: - 0.093, 0.037) to 0.001(95%CI: - 0.067, 0.069), and then to -0.074 (95%CI: -0.146, -0.003, P for trend = 0.0659). For every 10 ng/mL increase in serum 25(OH)D concentration corresponded to the β value change -0.058 (95%CI: -0.098, -0.017). This association was more pronounced in younger or overweight/obese individuals. Furthermore, in the longitudinal study, as the baseline quartile of serum 25(OH)D concentration increased, the estimated change of subsequent CMSRS indicated a decreasing trend, ranging from -0.085 (95%CI: -0.203, 0.032) to -0.166 (95%CI: - 0.285, - 0.046), and then to - 0.174 (95%CI: - 0.296, -0.053, P for trend = 0.0031). The mediating proportion of levels of NLR was 7.2%.

Conclusions: Higher serum 25(OH)D concentration is significantly associated with reduced CMSRS in children and adolescents, and adequate serum vitamin D levels play a prominent role in preventing long-term metabolic disorders, partly meditating by inflammation in peripheral blood.

Objective: Impaired skeletal muscle glycogen synthesis contributes to insulin resistance (IR). Aerobic exercise reported to ameliorate IR by augmenting insulin signaling, however the detailed mechanism behind this improvement remains unclear. This study investigated whether aerobic exercise enhances glycogen anabolism and insulin sensitivity via EGR-1/PTP1B signaling pathway in skeletal muscle of rats.

Methods: Sprague-Dawley rats fed a high-fat diet (HFD), and performed treadmill exercise training for 6-week. Oral glucose tolerance test was conducted to confirm the IR. Periodic Acid-Schiff (PAS) staining and anthrone colorimetry were used to assess the skeletal muscle glycogen. RT-qPCR, western blot, and immunofluorescence were used to detect the EGR-1/PTP1B pathway and associated signaling molecules.

Results: We found that exercise training significantly decreased blood glucose, insulin, and homeostasis model assessment for IR (HOMA-IR) against HFD-induced elevation. Decreased muscle glycogen content due to HFD was significantly restored after exercise training. Exercise training promoted mRNA expressions of Irs1, Akt, and Glut4, while inhibited Gsk-3β expression against HFD. Next, the decreased IRS1 (phosphorylated/total), AKT (phosphorylated/total), and GLUT4, and increased GSK-3β proteins with HFD were significantly reversed by exercise. Furthermore, HFD-induced overexpression of EGR-1 and PTP1B evidenced by mRNA, protein, and immunofluorescence intensity, were substantially inhibited by exercise, which may contribute to promote insulin sensitivity and glycogen anabolism.

Conclusions: Aerobic exercise training promotes insulin sensitivity and skeletal muscle glycogen synthesis in HFD-fed rats. The beneficial effects of exercise might be mediated by EGR-1/PTP1B signaling pathway in skeletal muscle, however further studies are necessary to confirm this mechanism.

Background: Many studies have explored the association between food intake and metabolic health. However, research on the association of consuming ultra-processed foods (UPFs) and metabolic health in children and adolescents remains unclear. The objective of our study was to investigate the relation between UPFs consumption and metabolic health status in Iranian adolescents with overweight/obesity.

Methods: For this cross-sectional study, we selected a sample of 203 adolescents (101 boys and 102 girls) with a mean age of 13.98 ± 1.61 who were chosen using a multistage cluster random sampling method. We collected data on their dietary patterns using a validated 147-item semi-quantitative food frequency questionnaire. Additionally, we measured anthropometric indices, blood pressure, fasting glucose, insulin, and lipid profiles. Participants were categorized to either metabolically healthy or unhealthy overweight/obesity (MHO and MUO) based on the International Diabetes Federation (IDF) and IDF/ Homeostasis Model Assessment Insulin Resistance (HOMA-IR) criteria.

Results: Our findings revealed that every 10 g/d increase in UPFs consumption was related to an elevated odds of MUO as defined by the IDF (OR = 1.14; 95%CI: 1.08-1.19) and IDF/HOMA-IR (OR = 1.14; 95%CI; 1.08-1.19) criteria, after controlling all confounders. The severity of this association varied among girls and boys and also among those with overweight and obesity according to the applied definitions. Moreover, each 10 g/d increase in daily UPFs intake was associated with increased odds of metabolic factors including hyperglycemia, hypertriglyceridemia, hypo-HDL-cholesterolemia, hypertension, and high insulin resistance levels.

Conclusion: These findings highlighted a direct association between higher UPFs consumption and MUO status among adolescents.

Background: This study aimed to explore the relationship between ultra-processed foods (UPFs) consumption and gut microbiota in patients with type 2 diabetes (T2D).

Methods: This cross-sectional study included 362 participants with T2D. UPFs consumption was assessed using a brief-type self-administered diet history questionnaire, quantified as the density of UPFs intake (g/1000 kcal). Gut microbial composition was evaluated via 16S rRNA gene sequencing. We investigated the association between gut microbiota, previously identified as relevant to T2D, and the density of UPFs intake using Spearman rank correlation coefficients. Multiple regression analysis, adjusting for age, sex, BMI, smoking status, exercise, and medication use, was conducted to further investigate these associations.

Results: The mean age of participants was 68 (63-74) years. The density of UPFs intake showed significant associations with Bifidobacterium (r = 0.11, p = 0.031), Lactobacillus (r = 0.11, p = 0.046), Ruminococcus (r = -0.12, p = 0.019), Roseburia (r = 0.11, p = 0.045). After adjusting for covariates in multiple regression analysis, Ruminococcus and Roseburia showed modest negative (β = -0.11, p = 0.038) and positive (β = 0.12, p = 0.033) correlations, with the density of UPFs intake among participants with T2D, respectively.

Conclusions: The density of UPFs intake was modestly inversely associated with Ruminococcus among patients with T2D and modestly positively associated with Roseburia.

Background: Abnormal uterine bleeding (AUB) is a common gynecological issue during reproductive years. Researches on how dietary diversity and patterns influence AUB in Chinese university students are limited, with some studies not clearly defining irregular menstruation. This research investigated the incidence of AUB according to the International Federation of Gynecology and Obstetrics criteria and sought to identify preventative factors via online questionnaires.

Methods: Food intake was assessed via factor analysis. This study analyzed the relationships among the dietary diversity score (DDS), dietary patterns, and AUB via univariate and multivariate binary logistic regression models and generalized additive models.

Results: The sample consisted of 698 students, with 434 (62.2%) having experienced AUB. Among them, 87 (20.05%) reported abnormal frequency, 250 (57.60%) reported irregular menstrual cycle, 41 (9.45%) reported prolonged duration, and 268 (61.75%) reported abnormal flow volume. A 1-unit increase in the DDS was linked to a 22% lower risk of AUB (odds ratio 0.78, 95% CI: 0.67, 0.90). A high DDS (≥ 6) was consistently associated with a lower risk of AUB across all the models. Using a two-piecewise linear regression model and recursive algorithm, a nonlinear, reverse L-shaped relationship was identified between the "low fat plant-based" dietary pattern factor score (FAC4) and AUB, with an inflection point of 1.45. Before this point, FAC4 had a protective effect on AUB (odds ratio 0.42, 95% CI: 0.21, 0.84).

Conclusions: Our study underscores the importance of a varied diet as a preventive measure for AUB, utilizing a DDS to quantify the relationship between dietary diversity and AUB. According to our findings, higher dietary diversity and moderate adherence to a "low fat plant-based" dietary pattern may be significant protective factors against AUB in university students.