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EXPOSURE AND EFFECTS OF TOXIC TRACE METALS IN BIRDS OF PAKISTAN 巴基斯坦鸟类中有毒微量金属的暴露及其影响
Pub Date : 2023-01-09 DOI: 10.57041/pjs.v74i4.762
S. Ashraf, N. A. Abbasi, S. Ahmad
Increasing concentration of trace metals in the environment and their subsequenthealth effects in living organisms has become a major threat particularly in developing parts of theworld. Therefore present study was designed to investigate the concentration of trace metals such asPb, Cd, Cr and Cu in liver, pectoral muscle, pelvic muscle and blood of two terrestrial (Bank Myna;Acridotheres ginginianus and Jungle Babbler; Turdoides striata) and two aquatic (Grey Heron; Ardeacinerea and Cattle Egret; Bubulcus ibis) bird species collected from the premises of Lahore, Pakistan.The detection frequency of trace metals in collected samples were highest in Cd (100%) followed byPb (83%), Cr (80%) and Cu (76.5%). Species revealed heterogeneous levels of metals in all organs. Ingeneral the mean concentration (µg/g) of trace metals in organs and blood of terrestrial speciesfollowed the pattern as Pb > Cu > Cr > Cd contrary to aquatic species in which the trend was Cu > Pb> Cr > Cd. Highest concentration of Pb (3.23 µg/g) was reported in pelvic muscle of jungle babbler,Cd (0.15 µg/g) in liver of bank myna, Cr (0.40 µg/g) in pelvic muscle of Cattle Egret and Cu (1.89µg/g) in liver of Cattle Egret. Whereas lowest concentration of Pb (0µg/g) and Cd (0.09 µg/g) wasreported in blood and pelvic muscle of Grey Heron respectively, Cr (0µg/g) in blood of Bank Myna,Grey Heron and Cattle Egret, Cu (0 µg/g) in blood of Grey Heron, Cattle Egret and Jungle Babbler.Concentration of metals varied significantly (P<0.05) among organs however, no significant difference(P>0.05) was observed among species except Cu(P<0.05). Further, no significant difference (P>0.05)of metals was observed between feeding guilds and habitat of the species. The increasing concentrationof toxic trace metals in the birds reflects deteriorating environmental health as a result of greater metalsexposure which must be reduced through proper legislation and strict implementation of laws.
环境中微量金属浓度的增加及其随后对生物体的健康影响已成为一个主要威胁,特别是在世界发展中地区。因此,本研究旨在研究两种陆生动物(Bank Myna; acridobs ginginianus和Jungle Babbler)的肝脏、胸肌、骨盆肌和血液中asPb、Cd、Cr和Cu等微量金属的浓度;杜鹃)和两个水生的(灰苍鹭;白鹭和白鹭;从巴基斯坦拉合尔地区收集的朱鹮(Bubulcus ibis)种鸟。样品中微量金属的检出率最高的是Cd(100%),其次是pb(83%)、Cr(80%)和Cu(76.5%)。各物种在所有器官中显示出不同水平的金属。陆生动物器官和血液中微量金属的平均浓度(µg/g)为Pb> Cu > Cr > Cd,水生动物器官和血液中微量金属的平均浓度为Cu > Pb> Cr > Cd。其中,丛林斑马鱼盆肌中Pb含量最高(3.23µg/g),岸八哥肝脏中Cd含量最高(0.15µg/g),牛白鹭盆肌中Cr含量最高(0.40µg/g),牛白鹭肝脏中Cu含量最高(1.89µg/g)。其中,银八哥、灰鹭和牛白鹭血液中微量铅(0µg/g)和Cd(0.09µg/g)最低,铜(0µg/g)最低,灰鹭、牛白鹭和丛林白鹭血液中微量铜(0µg/g)最低。除铜(Cu)在不同取食行业和生境间差异显著(P0.05)外,其他金属在不同种间差异显著(P0.05)。鸟类体内有毒微量金属浓度的增加反映出由于金属接触增加而导致环境健康恶化,必须通过适当立法和严格执行法律来减少这种情况。
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引用次数: 0
PHENOTYPIC TRENDS FOR MILK YIELD IN NILI RAVI BUFFALOES REGISTERED UNDER BULL MOTHER SCHEME AND AT LIVESTOCK EXPERIMENT STATION BAHADURNAGAR- OKARA 牛母计划和bahadurnagar - okara牲畜试验站登记的尼利拉维水牛产奶量的表型趋势
Pub Date : 2023-01-09 DOI: 10.57041/pjs.v74i4.759
A. Manan
Efficient breeding plans depend upon the availability of reliable and accurate data.To evaluate the productive and reproductive performance of the Nili-Ravi buffalo, registered under thebull mother scheme, was evaluated from 1980-2020. General Linear Model is used to study the effectof phenotypic characters on milk yield i.e., year of calving, age at first calving, and dam’s parity onproductive traits under consideration. The lactation records of all parity are used for the analysis. Thedata on pedigrees, breeding, and performance records through analysis of variance procedure showsstatistically significant (P < 0.05) differences for the effect of 305 days milk yield and lactation length.The Least Squares Mean for lactation milk yield, age at first calving and lactation length are 2108.62 ±12.80 liters, 1574.49 ± 8.1 and 295.50 ± 1.47 days, respectively. The inclusive results depict thatbuffaloes calve in the winter season have the highest and most significant total milk yield. The data setcould be used as a phenotype for genome-wide association studies as a reference population.
有效的育种计划取决于能否获得可靠和准确的数据。为了评估在公牛母亲计划下注册的Nili-Ravi水牛的生产和繁殖性能,从1980年到2020年进行了评估。采用一般线性模型研究了不同表型性状对产奶量的影响,即产犊年份、初产犊龄和胎次对产奶量的影响。所有胎次的泌乳记录用于分析。通过方差分析,家谱、育种和生产性能记录的数据显示,305天产奶量和泌乳时间的影响差异有统计学意义(P < 0.05)。泌乳产奶量、初产日龄和泌乳时间的最小二乘平均值分别为2108.62±12.80 l、1574.49±8.1和295.50±1.47 d。综合结果显示,冬季小牛的总产奶量最高、最显著。该数据集可作为全基因组关联研究的表型作为参考群体。
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引用次数: 0
DEM BASED COMPUTATION OF TOPOGRAPHIC SURFACE ROUGHNESS TO REVEAL INCISION IN GILGIT-BALTISTAN,PAKISTAN 基于Dem的巴基斯坦吉尔吉特-巴尔蒂斯坦地形表面粗糙度计算揭示切口
Pub Date : 2023-01-05 DOI: 10.57041/pjs.v65i1.724
S.R. Ahmad
This research investigates interrelationship between landscape evolution and neotectonics in the Gilgit-Baltistan (GB) region based on surface roughness (SR). We analyzed the topographic surface features such as flat area, surface area and surface ratio using Shuttle RadaTopographic Mission-Digital Elevation Model (SRTM DEM) with a spatial resolution of 90 meters. The objective is to make a SR map and to identify areas influenced by neotectonics. The DEM based spatial distribution of slope gradients and slope orientations along with the aspect map were combined to portray the vertical dissection. Lower values of vertical dissection indicate relatively flat topographic basins which generally mean a region of with intermediate slope gradient and slope orientation. Areas with high vertical dissection values correspond to sharp changes in the slope gradients and slope orientations, which could be due to abrupt scrap edges (possibly neotectonic or lithological control). Higher vertical dissection values in GB are wide spread and are oriented SE- NW in Ghanche, Skardu, Hunza, Gilgit and northern Ghizer districts along Karakorum, South Pamirs and Hindu-Kush ranges. While a few higher roughness values are aligned NE- SW in Diamir and Gilgit districts along the Raikot Fault. The surface roughness map is capable of identifying deeply incised valleys, tectonic uplifts and depressions.
基于地表粗糙度(SR)研究了吉尔吉特-巴尔蒂斯坦(GB)地区景观演化与新构造的相互关系。利用空间分辨率为90 m的SRTM DEM (Shuttle RadaTopographic Mission-Digital Elevation Model)分析了平坦面积、比表面积和比表面积等地形表面特征。目的是绘制SR图,确定受新构造影响的地区。将基于DEM的坡度和坡向空间分布与坡向图相结合来描绘垂直解剖。垂向解剖值较低表明地形盆地相对平坦,一般为坡度和坡向中等的区域。垂直解剖值高的地区对应于斜坡梯度和斜坡方向的急剧变化,这可能是由于突然的碎屑边缘(可能是新构造或岩性控制)。在喀喇昆仑山脉、南帕米尔高原和兴都库什山脉沿线的Ghanche、Skardu、Hunza、Gilgit和Ghizer北部地区,高垂直解剖值分布广泛,呈SE- NW取向。而在迪亚米尔和吉尔吉特地区沿莱科特断层的东北-西南方向有一些较高的粗糙度值。地表粗糙度图能够识别深切谷、构造隆升和构造坳陷。
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引用次数: 0
EXTRACTION AND EVALUATION OF A NATURAL DYE FROM BISTORTA AMPLEXICAULE 一种天然染料的提取及评价
Pub Date : 2023-01-05 DOI: 10.57041/pjs.v69i2.355
A. Fatima
Natural pigments extracted from plants are raised as a vital substitute to artificial dyes. The present study was conducted to extract pigment from Bistorta amplexicaule and evaluate its dyeing property, antimicrobial activity and toxicity. The extraction was done by maceration and sonication methods with 7.5% and 5% yield, respectively. The extracted pigment appeared bright- amber colored which was used for dyeing fabrics and food using traditional methods. The suitable dying was achieved using 4g dye powder in 100ml water for fabrics and 250mg in 26.2g of rice. The extract did not indicate antibacterial activity against Staphylococcus aureus, Klebsiella sp., Escherichia coli and Serratia marcescens by disc-diffusion method. Dye extract using albino mice assay did not show any mortality throughout the study period (15 days). The natural plant pigment extracted from B. amplexicaule had a potential as natural dye for food and fabric.
从植物中提取的天然色素被认为是人工染料的重要替代品。本研究从大花蓟马中提取色素,并对其染色性能、抗菌活性和毒性进行了评价。采用浸渍法和超声法提取,得率分别为7.5%和5%。提取的色素呈琥珀色,用传统方法染色织物和食品。织物用100毫升水染4克染料粉,26.2克大米染250毫克染料粉。圆盘扩散法对金黄色葡萄球菌、克雷伯氏菌、大肠杆菌和粘质沙雷氏菌均无抑菌活性。在整个研究期间(15天),使用白化小鼠试验的染料提取物未显示任何死亡。从大花莲中提取的天然植物色素具有作为食品和织物天然染料的潜力。
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引用次数: 0
RESPONSE OF Ty-RESISTANT TOMATO (Solanum lycopersicum) CULTIVARS TO BEGOMOVIRUSES AND THEIR GAS CHROMATOGRAPHY MASS SPECTROMETERY ANALYSIS 抗ty番茄品种对begomovirus的反应及其气相色谱质谱分析
Pub Date : 2023-01-05 DOI: 10.57041/pjs.v68i4.254
R. Siddique
Tomato (Solanum lycopersicum) production is affected by different diseases andTomato yellow leaf curl virus (TYLCV) is the most prevalent and devastating begomovirus. Tyresistant cultivars were developed by Asian Vegetable Research and Development Center (AVRDC)Taiwan, using different combinations of Ty genes to observe pyramiding effects on resistance againstvarious Begomoviruses. Cultivars containing Ty genes were grown in glass house at 65% relativehumidity, 16 hours Dark and 8 hours Light, at 26 oC. At the age of 5 to 6 leaves, plants were exposed toviruliferous whitefly and were kept for ~40 days. Symptoms were observed every 10 days postexposure. Among nine Ty-resistant and one native (Nagina) cultivars, three were resistant i.e., R1, R6and R9, two mild to moderately resistant i.e., R2 and R10, three i.e., R7, R8 and R14 very mildresistant while one Ty cultivar i.e., R15 along with susceptible (Nagina) variety showed no resistance.Nagina (susceptible) and R6 (resistant) cultivars were analyzed by Gas Chromatography MassSpectrometry (GCMS). Among Ty cultivars (Ty-2, Ty-3 and Ty-5) genes harbouring cultivars showedbetter resistance and GCMS analysis lead to identification of bioactive compounds associated withresistant (R6) cultivar.
番茄(Solanum lycopersicum)的生产受到多种病害的影响,其中番茄黄卷叶病毒(TYLCV)是最普遍和最具破坏性的begomvirus。台湾亚洲蔬菜研究与发展中心(AVRDC)利用不同的Ty基因组合,培育了抗tyy的品种,观察了对各种begomovirus抗性的金字塔效应。含Ty基因的品种在相对湿度65%、暗16小时、光8小时、温度26℃的玻璃室内培养。在5 ~ 6叶时,将植株暴露于毒粉虱中,保存40天。接触后每10天观察一次症状。9个品种中,抗性品种R1、r6、R9 3个,轻度至中度抗性品种R2、R10 2个,极轻度抗性品种R7、R8、R14 3个,1个品种R15和易感品种Nagina均无抗性。采用气相色谱-质谱法(GCMS)对敏感品种Nagina和抗性品种R6进行分析。在Ty-2、Ty-3和Ty-5 3个品种中,含有基因的品种表现出较好的抗性,GCMS分析鉴定出了抗性品种R6的相关活性物质。
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引用次数: 0
EFFICIENCY ANALYSIS OF OPEN FIELD TOMATO PRODUCTION IN PUNJAB: A NON PARAMETRIC DATA ENVELOPMENT ANALYSIS APPROACH 旁遮普露天番茄生产效率分析:一种非参数数据包络分析方法
Pub Date : 2023-01-05 DOI: 10.57041/pjs.v69i3.537
Q. Ali
This study was designed to calculate production efficiency (technical, economic and allocative) and determinants of inefficiency in open field tomato production in Punjab, Pakistan. Simple random sampling was used for primary data collection from 70 farmers in 2014. Data envelopment analysis explored the mean of technical (79.4%), allocative (58.1%) and economic (45.9%) efficiency. It showed the capability of 21.6 and 41.9% reduction in inputs and total cost, respectively with same output and technology. For sub groups, technical efficiency (92.1%) was more for medium farmer while allocative (74.2%) and economic efficiency (63.8%) was higher for large farmer. Tobit model explored the negative impact of education, experience, extension services and credit availability on inefficiency. Impact on inefficiency was positive for family size, area and vegetable market distance.
本研究旨在计算巴基斯坦旁遮普省露天番茄生产的生产效率(技术、经济和配置)和低效率的决定因素。2014年采用简单随机抽样的方法对70名农民进行初步数据采集。数据包络分析探讨了技术效率(79.4%)、配置效率(58.1%)和经济效率(45.9%)的平均值。在相同的产量和技术条件下,投入和总成本分别降低21.6%和41.9%。从亚群体来看,中型农户的技术效率(92.1%)更高,大型农户的配置效率(74.2%)和经济效率(63.8%)更高。Tobit模型探讨了教育、经验、推广服务和信贷可得性对低效率的负面影响。家庭规模、面积和菜市场距离对效率低下的影响均为正。
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引用次数: 2
A LITERATURE REVIEW ON ALKALI SILICA REACTIVITY OF CONCRETE IN PAKISTAN 巴基斯坦混凝土碱硅反应性的文献综述
Pub Date : 2023-01-05 DOI: 10.57041/pjs.v68i1.114
M. J. Munir
Pakistan has variety of topographic features including planes, sub-mountainous andmountainous ranges. These mountainous and sub-mountainous ranges possess a vast potential forconcrete aggregates. However, alkali silica reactivity has largely been observed in the past, due toreactive nature of these aggregates. This study was planned to explore the possible causes of alkalisilica reaction (ASR) and their consequences in the environment of Pakistan. Several materials can beused economically to mitigate alkali silica expansion. Among all possible remedies, use of granulatedblast furnace slag is the most effective and economical option to control ASR. Use of other materialssuch as calcined or bentonite clay, rice husk ash, bagasse ash and silica fume may be effective againstASR.
巴基斯坦有多种地形特征,包括平原、亚山地和山脉。这些山区和亚山区具有巨大的混凝土集料潜力。然而,由于这些聚集体的反应性,碱二氧化硅的反应性在过去已经被广泛观察到。本研究旨在探讨巴基斯坦环境中碱硅反应(ASR)的可能原因及其后果。有几种材料可以经济地缓解碱硅膨胀。在所有可能的补救措施中,使用粒状高炉矿渣是控制ASR最有效和最经济的选择。使用其他材料,如煅烧或膨润土粘土,稻壳灰,甘蔗渣灰和硅灰可能对asr有效。
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引用次数: 3
PROBABILITY MODELING OF LOW FLOWS AT DIFFERENT SITES OF INDUS BASIN IN PAKISTAN USING L-MOMENTS AND TL-MOMENTS 利用l -矩和l -矩对巴基斯坦印度河流域不同地点低流量的概率模拟
Pub Date : 2023-01-05 DOI: 10.57041/pjs.v68i1.139
None I. Ahmad
At-site Frequency Analysis (ASFA) of low flow was carried out for nine sites ofIndus basin in Pakistan. In the present study, 10-day annual low flow series were analyzed by robustestimation methods such as Method of L-moment (ML) and TL-moment (MTL) to identify best fitprobability distributions for each site. Best distribution for each site was identified using differentgoodness-of-fit Tests (GFT). No single probability distribution was declared as the best-fit distributionfor all sites included in the plan. The GFT results indicated GPA was the most appropriate distributionfor most of the sites followed by GLO and GEV distributions. On comparison, it was found that formost of the sites ML was best estimation method and for others MTL. For ASFA, the quantiles of bestfit distribution were also estimated. It was found that estimated low flows based on fitted distributionwere in close agreement with observed flows.
对巴基斯坦findus盆地的9个站点进行了低流量现场频率分析(ASFA)。本研究利用l -矩法(ML)和tl -矩法(MTL)等鲁棒估计方法分析了10天的年低流量序列,以确定每个站点的最佳拟合概率分布。使用不同的拟合优度检验(GFT)确定每个站点的最佳分布。没有一个单一的概率分布被宣布为计划中所有地点的最佳拟合分布。GFT结果表明,GPA是大多数站点最合适的分布,其次是GLO和GEV分布。比较发现,对于大多数站点,ML是最好的估计方法,而对于其他站点,MTL是最好的估计方法。对于ASFA,还估计了最佳拟合分布的分位数。结果发现,根据拟合分布估计的低流量与观测流量非常吻合。
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引用次数: 0
REMOTE SENSING AND GIS APPLICATIONS FOR MONITORING AND ASSESSMENT OF THE URBAN SPRAWL IN FAISALABAD-PAKISTAN 遥感和地理信息系统在巴基斯坦费萨拉巴德城市扩张监测和评估中的应用
Pub Date : 2023-01-05 DOI: 10.57041/pjs.v64i3.535
M. N. Bhalli, A. Ghaffar, S. A. Shirazi
Urban sprawl is a global phenomenon of present era and is mostly taking place in less developed countries. In LDCs there is a consistent and vibrant trend among the people to agglomerate in mega cities and large urban centers. This phenomenon has gained the attention of researchers in the fields of urban geography, environmental studies and city & regional planning. Keeping in view its importance, an attempt has been made to explore the use of geo-informatics techniques for the assessment of urban sprawl in Faisalabad city. The result inferred from the classified Landsat image for year 1980 revealed that the total built-up area of Faisalabad was 5661 hectors (26 per cent of total) while year 2010 classified Landsat image depicts that the total built-up area was 9480.44 hectors (44 per cent) of the City. The total areal change for 30 years research span (1980-2010) was 44 per cent in built up land, contrary to this non built-up /open area decreased to 32 per cent during the same period. The total area in 1980 was 102 sq. km while the city grew up to 213 sq. km by year 2010. This appreciable change in built up lands was at the expanse of fertile agricultural tracts once part of the fringe of Faisalabad city.
城市扩张是当今时代的全球性现象,主要发生在欠发达国家。在最不发达国家,人们向特大城市和大型城市中心聚集的趋势一贯而活跃。这一现象引起了城市地理学、环境研究、城市与区域规划等领域研究人员的关注。考虑到它的重要性,已经尝试探索利用地理信息学技术来评价费萨拉巴德市的城市蔓延。从1980年的分类Landsat图像推断出的结果显示,费萨拉巴德的总建成区面积为5661公顷(占总面积的26%),而2010年的分类Landsat图像显示,该市的总建成区面积为9480.44公顷(44%)。在30年的研究跨度(1980-2010)中,建筑用地的总面积变化为44%,而非建筑/开放面积在同一时期下降到32%。1980年的总面积为102平方公里。而城市面积扩大到213平方公里。到2010年。建筑用地的这种明显变化发生在曾经属于费萨拉巴德市边缘的肥沃农田的广阔地区。
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引用次数: 11
“ESTIMATION OF RADICAL SCAVENGING POTENTIAL OF SELECTED MEDICINAL PLANTS OF LAHORE, PAKISTAN” 巴基斯坦拉合尔部分药用植物自由基清除能力的评估
Pub Date : 2023-01-05 DOI: 10.57041/pjs.v69i3.559
S. K. Malik
In the current research, in vitro antiradical potential of some medicinal trees of Lahore, Pakistan was investigated. Various fruits and pods of 25 medicinal plants (Prosopis juliflora, Carissa carandas, Ceiba speciosa, Heterophragma adenophyllum Cestrum diurnum Jacaranda mimosifolia, Diospyros malabarica, Terminalia bellerica, Ficus lyrata, Diospyros peregrine, Cinnamomum verum, Erysimum cheiri, Buchanania lanzan, Withania somnifera, Fagonia arabica, Berberis lycium, Strychnos potatorum, Matthiola incana, Ziziphora tenuior, Centaurea behen, Rosa indica, Punica granatum, Lodoicea maldivica, Cassia absus, Celastrus paniculatus) were extracted by ethanol. Different concentrations of fruit extracts (1, 0.5, 0.25, 0.125 mg/mL) were prepared to evaluate in vitro antioxidant activity. The DPPH (2,2-diphenyl-1-picrylhydrazyl) free radical scavenging method was performed and compared with standards like Vitamin E, BHT (Butylated hydroxytoluene) and ascorbic acid. Many plant extracts exhibited 80% of DPPH radical scavenging activity. F. Arabica, S. potatorum, M. incana, P. granatum, C. behen, W. somnifera, T. bellerica, and H. adenophyllum showed significant anti-radical potential (98.4±0.52, 96.40±0.52, 96.42±0.51, 94.58±0.52, 94.47±0.50, 94.65±0.56, 94.55±0.50, 91.42±0.51% inhibition, respectively) at 1 mg/mL equivalent to standards i.e. Vitamin E, BHT and ascorbic acid inhibition (88.25±0.2, 78.1±0.3 and 40.51±0.17%), respectively. Moreover, the dose dependent activity was observed as decline in the percentage inhibition at lower extracts concentration. The 50% inhibitory concentration (IC50) was also determined for the extracts that varied from 0.014± 0.001 (W. somnifera and J. mimosifolia) to 2.69±0.001 (B.lanzan) mg/mL depending on the sample extracts. The natural antioxidants in plants may be used in foods and natural products as a substitute to synthetic antioxidants, which have side effects.
本研究对巴基斯坦拉合尔地区几种药用树木的体外抗自由基活性进行了研究。25种药用植物的各种果实和荚果(黄豆豆、Carissa carandas、Ceiba speciosa、异叶花椰菜、黄花椰菜、黄花椰菜、黄花椰菜、葡萄花椰菜、葡萄花椰菜、葡萄花椰菜、阿拉伯烟草、枸杞小檗、土豆马钱子、金盏花、金盏花、半人马花、印度玫瑰、石榴、金盏花、黑穗花、黑穗花等)。乙醇法提取蛇芹。制备不同浓度的水果提取物(1、0.5、0.25、0.125 mg/mL),评价其体外抗氧化活性。采用DPPH(2,2-二苯基-1-苦基肼基)自由基清除法,并与维生素E、BHT(丁基羟基甲苯)、抗坏血酸等标准进行比较。许多植物提取物具有80%的DPPH自由基清除活性。在相当于维生素E、BHT和抗坏血酸抑制标准(分别为88.25±0.2、78.1±0.3和40.51±0.17%)的1 mg/mL条件下,Arabica、S. potatorum、M. incana、P. granatum、C. behen、W. somnifera、T. bellerica和H. adenophyllum的抗自由基活性分别为98.4±0.52、96.40±0.52、96.42±0.51、94.58±0.52、94.47±0.50、94.65±0.56、94.55±0.50、91.42±0.51%。此外,剂量依赖性活性观察到,在较低的提取物浓度的百分比抑制下降。50%抑制浓度(IC50)在0.014±0.001 mg/mL和2.69±0.001 mg/mL之间变化。植物中的天然抗氧化剂可作为合成抗氧化剂的替代品用于食品和天然产品中,但合成抗氧化剂有副作用。
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引用次数: 1
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Pakistan journal of science
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