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BIOREMEDIATION OF HEXAVALENT CHROMIUM BY FREE AND IMMOBILIZED BIOMASS OF CHLORELLA AND PSEUDOMONAS 小球藻和假单胞菌游离和固定化生物量对六价铬的生物修复
Pub Date : 2023-01-05 DOI: 10.57041/pjs.v69i3.533
S. Akram
Hexavalent chromium is a toxic heavy metal and carcinogen. The purpose of present study was to analyze Cr(VI) resistant bacterial strain S7 in combination with Cholera for observing the mutualistic relationship for remediation processes. By 16S rRNA sequencing technique, the strain S7 was identified to be Pseudomonas species (KR095629). It was able to tolerate Cr(VI) stress up to 600μg/mL and showed an optimum Cr(VI) reduction of 100μg/mL. Sodium alginate (2%) was used for immobilization of S7 and Chlorella. Chromium (VI) removal by immobilized cells of S7 and Chlorella was 4.5mg/g and 6.20mg/g, with reduction potential of 58% and 60%, respectively. A direct relation between Cr(VI) concentration and root length of Chlorella was observed. Maximum root length was observed for Chlorella and S7 i.e. 1.9cm at 500μg/ml Cr(VI) concentration. The presence of several absorption peaks in the Fourier transform infrared spectroscopy (FTIR) of the biomass indicated its complex nature. This technique revealed the involvement of hydroxyl group, N-H and C- O group. The Cr(VI) was effectively removed by bacterial strain when used with Chlorella.
六价铬是一种有毒的重金属和致癌物质。本研究的目的是分析Cr(VI)耐药菌株S7与霍乱的联合,以观察修复过程的相互关系。通过16S rRNA测序技术鉴定菌株S7为KR095629假单胞菌。它能耐受高达600μg/mL的Cr(VI)胁迫,Cr(VI)降低的最佳浓度为100μg/mL。用2%海藻酸钠固定化S7和小球藻。S7和小球藻固定化细胞对铬(VI)的去除率分别为4.5mg/g和6.20mg/g,还原电位分别为58%和60%。Cr(VI)浓度与小球藻根长有直接关系。在500μg/ml Cr(VI)浓度下,小球藻和S7的最大根长为1.9cm。生物质的傅里叶变换红外光谱(FTIR)中存在多个吸收峰,表明其复杂性质。该技术揭示了羟基、N-H和C- O基团的参与。与小球藻共同使用时,菌株对Cr(VI)的去除效果较好。
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引用次数: 0
PREVALENCE, BACTERIOLOGY AND ANTIBIOTIC SENSITIVITY PROFILE OF SUB- CLINICAL MASTITIS IN GOATS IN DISTRICT JHELUM. jhelum地区山羊亚临床乳腺炎患病率、细菌学及抗生素敏感性分析。
Pub Date : 2023-01-05 DOI: 10.57041/pjs.v69i3.574
Mhussain
In this study the prevalence of sub-clinical mastitis was determined in milk samples collected from goats of different breeds from district Jhelum. Surf field mastitis test and White side test were used for screening. Out of 500 animals tested, 14.2% animals were found positive for sub-clinical mastitis. Out of the positive samples, 12.2% were having infection in single, while, 2.0% were having infection in both halves of the udder. Beetle breed was infected at the highest rate i.e. 22.54% following desi breed i.e. 10.40% and teddy breed i.e. 8.80%, respectively. Prevalence of mastitis increased with increase in number of parity i.e. 8.5% up to 2nd parity and 19.76% up to 3rd parity. Bacteriological profile showed that Staphylococcus aureus was the most common pathogen i.e. 51.43% isolated. Antibiotic sensitivity test revealed that most of the isolated bacteria i.e. 93.20% were sensitive to Gentamicin.
在这项研究中,亚临床乳腺炎的患病率确定了从Jhelum地区不同品种的山羊收集的牛奶样本。采用冲浪场乳腺炎试验和白侧试验进行筛选。在测试的500只动物中,14.2%的动物被发现患有亚临床乳腺炎。在阳性样本中,12.2%的人单侧感染,2.0%的人双侧感染。甲虫品种感染率最高,为22.54%,其次是德西品种(10.40%)和泰迪品种(8.80%)。乳腺炎的患病率随着胎次的增加而增加,即到第二次胎次为8.5%,到第三次胎次为19.76%。病原菌以金黄色葡萄球菌最多,占51.43%。抗生素敏感性试验显示,大多数分离菌(93.20%)对庆大霉素敏感。
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引用次数: 1
PHYSICAL AND CHEMICAL FACTORS AFFECTING CHICKEN MEAT COLOR 影响鸡肉色泽的理化因素
Pub Date : 2023-01-05 DOI: 10.57041/pjs.v71i2.268
A. Qamar
The expectation in term of meat quality characters has been increased due to consumer demand in the last few years. The quality of meat and meat products have a major concern and can never be compromised in any situation. Sensory qualities such as appearance, texture, juiciness, wateriness, firmness, tenderness, odor and flavor are the main factors, which influence the final meat purchasing decision of the consumer. It is well known that the darker color of thigh meat is due to the higher volume of myoglobin and haem pigments, as well as a higher pH when compared to breast meat. Killing older birds increases myoglobin amount in the meat and selection of breeds for higher breast meat production may be involved. Consuming a wheat-based diet tends to reduce the color of breast meat but has less effect on the thigh meat. After killing, biochemical variations, initiating the conversion of muscle to meat, regulate final meat quality. Heritability estimates for meat quality attributes in broilers are surprisingly high making genetic selection a best way for upgrading of broiler meat quality. However, the utilization of advanced new technologies, development of efficient and new processing methods will help to improve the chicken meat quality.
在过去的几年里,由于消费者的需求,对肉质特性的期望有所提高。肉类和肉制品的质量是一个重大问题,在任何情况下都不能妥协。外观、质地、多汁性、水性、紧实度、嫩度、气味和风味等感官品质是影响消费者最终购买肉类决策的主要因素。众所周知,大腿肉的颜色较深是由于与胸肉相比,大腿肉的肌红蛋白和血红素含量较高,以及pH值较高。宰杀年龄较大的禽类会增加肉中肌红蛋白的含量,并可能涉及到为提高胸肉产量而选择品种。以小麦为基础的饮食往往会降低胸肉的颜色,但对大腿肉的影响较小。屠宰后,生物化学变化,开始肌肉到肉的转化,调节最终的肉品质。肉鸡肉质性状的遗传力估计惊人地高,使遗传选择成为肉鸡肉质升级的最佳途径。然而,利用先进的新技术,开发高效的新加工方法将有助于提高鸡肉的品质。
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引用次数: 8
QUINOA IS BENEFICIAL TO THE COMPREHENSIVE NUTRITIONAL VALUE OF POTENTIAL HEALTH 藜麦具有有益健康的综合营养价值
Pub Date : 2023-01-05 DOI: 10.57041/pjs.v71i2.272
S.G. Mohyuddin
The quinoa herb belongs to the family of Chenopodiaceae in which spinach and beet also originate. It is South America local herb, and Chenopodium has 250 different kinds all over the world. Quinoa is a pseudo-cereal with high phytochemical substances verified biological and nutritional significance. Quinoa is consumed and used as daily essential food throughout the world due to its higher amount of protein in relation to general cereals. Quinoa contains ascorbic acid, vitamin E tocopherols and B complex vitamins niacin, folic acid, thiamine. Minerals (calcium, potassium, iron, manganese, magnesium, phosphorus), it also contains isoflavones and the best type of triglyceride. A functional perspective of quinoa plant is of great importance as are being used in the treatment and prevention of different health problems. The study will elaborate physiochemical, rheological and functional properties of quinoa. Bread will be prepared from quinoa and its textural, sensory attributes and chemical composition will be determined by standard method and the result obtained from the recent study will be analyzed according to standard procedure
藜麦属藜科,菠菜和甜菜也起源于藜科。它是南美洲的地方草本植物,全世界有250个不同的种类。藜麦是一种具有高生物和营养价值的植物化学物质的伪谷物。藜麦因其蛋白质含量高于一般谷物而被全世界作为日常必需食物食用。藜麦含有抗坏血酸,维生素E生育酚和复合维生素B,烟酸,叶酸,硫胺素。矿物质(钙、钾、铁、锰、镁、磷),它还含有异黄酮和最佳类型的甘油三酯。藜麦植物的功能观点是非常重要的,因为它被用于治疗和预防各种健康问题。本研究将详细阐述藜麦的理化、流变学和功能特性。面包将由藜麦制成,其质地、感官属性和化学成分将按标准方法测定,最近的研究结果将按标准程序进行分析
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引用次数: 6
COMPARATIVE PROFILING OF PHYTOCHEMICAL CONTENTS AND RADICAL SCAVENGING POTENTIAL IN SOME SPECIES OF FAMILY MORACEAE 桑科几种植物化学成分及自由基清除能力的比较分析
Pub Date : 2023-01-05 DOI: 10.57041/pjs.v69i2.346
K. Zahid
The current study was carried out for comparative estimation of phytochemicals, anti-radical properties and half maximal inhibitory concentration (IC50) of seven species of family Moraceae using 2, 2-Diphenyl-1-Picrylhydrazyl assay. Methanolic (70%) extraction of crude leaves extracts was done via Double Maceration technique. Quantitative assay for determining the radical scavenging activity of DPPH as well as IC50 value of seven species of family Moraceae was observed. Results showed the presence of all major classes of phyto-compounds in seven species of family Moraceae, maximum percent inhibition radical scavenging activity was observed by Ficus infectoria (96±0.21) while minimum in Ficus macrophylla (scavenging >79.08%). Half maximal inhibitory concentration (IC50) of all the seven species of family Moraceae was done by Graph pad prism 5.04 software. Concentration dependent percent inhibition was observed in all the species of Family Moraceae. However all the selected species had significant antioxidant potential which indicated the pharmaceutical uses of all the species under study on commercial scale in near future.
采用2,2 -二苯基-1-苦酰基肼基测定法,对桑科7种植物的化学成分、抗自由基性能和半最大抑制浓度(IC50)进行了比较研究。采用双浸渍法对粗叶提取物进行甲醇(70%)提取。对7种桑科植物的DPPH自由基清除能力和IC50值进行了定量测定。结果表明,7种桑科植物均含有主要种类的植物化合物,其中榕属植物对自由基的清除率最高(96±0.21%),而大叶榕属植物的清除率最低(>79.08%)。采用graphpad prism 5.04软件对桑科7种病原菌的半数最大抑菌浓度(IC50)进行测定。桑科所有种均有浓度依赖性抑制。所选树种均具有显著的抗氧化潜力,预示着所选树种在不久的将来具有商业化的药用价值。
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引用次数: 0
AUTOMATED CLASSIFICATION OF HAIR CARE PLANTS USING GEOMETRICAL AND TEXTURAL FEATURES FROM LEAF IMAGES: A PATTERN RECOGNITION BASED APPROACH 利用叶片图像的几何和纹理特征对护发植物进行自动分类:基于模式识别的方法
Pub Date : 2023-01-05 DOI: 10.57041/pjs.v68i4.201
A. Shaukat
Automated classification plays a vital role in content based image retrieval systemsin addition to many more. Inter-class similarity and intra-class dissimilarity is the main challengeposed by leaf classification. This research work proposed a plant classification system using texturaland geometrical features from leaf images. Six classification models, among which three wereensemble methods, were considered to evaluate the accuracy of proposed technique. Train and teststrategy was adopted to evaluate the performance of different classifiers. Experimental results showedthat the proposed technique outperformed the state of the art. Moreover, it was observed that texturalfeatures outperformed geometrical features. The best accuracy achieved with textural features was100%, whereas it was 98.8% when geometrical features were used. SVM, IBk and Random Treeremained the best classifiers in leaf identification using both types of features. Hence, textural andgeometrical features could be effectively used for plant classification
自动分类在基于内容的图像检索系统中起着至关重要的作用。类间相似性和类内不相似性是叶片分类面临的主要挑战。本研究提出了一种利用叶片图像纹理和几何特征的植物分类系统。采用6种分类模型,其中3种为集成方法,对所提方法的准确性进行了评价。采用训练和测试策略来评估不同分类器的性能。实验结果表明,所提出的技术优于目前的技术水平。此外,观察到纹理特征优于几何特征。使用纹理特征获得的最佳准确率为100%,而使用几何特征获得的准确率为98.8%。支持向量机、IBk和随机树在两种特征的叶子识别中都是最好的分类器。因此,纹理和几何特征可以有效地用于植物分类
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引用次数: 0
AN EFFICIENT ALGORITHMIC SOLUTION FOR HIGH PAYLOAD GOOD IMPERCEPTIBILITY HYBRID IMAGE STEGANOGRAPHY 一种有效的高负载、高隐蔽性混合图像隐写算法
Pub Date : 2023-01-05 DOI: 10.57041/pjs.v69i2.340
A. Murtaza
A hybrid steganographic technique has been proposed to enlarge the size of secret message and to hide several images in one cover image. Initially, the secret images were compressed using JPEG 2000 before embedding them to the cover image. Second least significant bit plane of cover image was entirely replaced by secret message while tri-ways-pixel-value-differencing (TPVD) was used to hide secret message in remaining seven-bit cover image. The quality of stego image was enhanced by residual image coding (RIC). The cover image was divided in four sub-images, three of them provided cover for secret message using proposed technique while fourth sub-image was used to embed RIC of stego image using only TPVD, to ensure better quality in terms of peak signal to noise ratio (PSNR). Proposed technique provided the opportunity to transmit secret data without detection by different steganalysis techniques like dual statistics steganalysis. Experimental results verified that proposed technique achieved high payload with better image quality than TPVD scheme.
提出了一种混合隐写技术,以扩大秘密信息的大小,并在一个封面图像中隐藏多个图像。最初,秘密图像在嵌入到封面图像之前使用JPEG 2000进行压缩。将封面图像的第二低有效位平面全部替换为秘密信息,并在剩余的7位封面图像中使用三路像素值差分(TPVD)隐藏秘密信息。残差图像编码(RIC)增强了隐写图像的质量。将掩蔽图像划分为4个子图像,其中3个子图像利用所提出的技术对秘密信息进行掩蔽,而第4个子图像仅利用TPVD来嵌入隐写图像的RIC,以保证峰值信噪比(PSNR)的更好质量。该技术提供了传输机密数据而不被其他隐写分析技术(如双统计隐写分析)检测的机会。实验结果表明,该技术具有较高的有效载荷,且图像质量优于TPVD方案。
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引用次数: 0
OPTIMIZATION OF BIODIESEL PRODUCTION THROUGH BASE-CATALYZED METHANOLYSIS OF CANTALOUPE SEED OIL 哈密瓜籽油碱催化甲醇裂解生产生物柴油的优化研究
Pub Date : 2023-01-05 DOI: 10.57041/pjs.v69i2.361
T. Akhtar
Biodiesel represents a suitable alternative to the petroleum-derived diesel fuel as it is renewable and can be used directly in compression-ignition engines. Present work was aimed at extraction of cantaloupe seed oil and its application to the production of biodiesel through base- catalyzed transesterification using four different basic catalysts viz. NaOH, NaOCH3, KOH and KOCH3. The percent yield of biodiesel afforded by these catalysts was 94.5, 88.0, 85.5 and 89.5%, respectively. The results indicated that NaOH was the best catalyst for transesterification of cantaloupe seed oil. Further, optimization of the process variable, including methanol to oil mole ratio, reaction temperature and amount of catalyst (NaOH) was carried out. The optimum levels of these variables that afforded maximum biodiesel yield were found to be 6:1 molar ratio of methanol to oil, 60 oC reaction temperature and one percent wt/wt catalyst loading. From the results obtained in this study,it was concluded that cantaloupe seed oil can be successfully utilized for the production of biodiesel as a cost-effective feedstock.
生物柴油是石油衍生柴油的合适替代品,因为它是可再生的,可以直接用于压缩点火发动机。研究了在NaOH、NaOCH3、KOH和KOCH3四种碱性催化剂的催化下,哈密瓜籽油的提取及其在生物柴油生产中的应用。这些催化剂的生物柴油收率分别为94.5%、88.0、85.5%和89.5%。结果表明,氢氧化钠是哈密瓜籽油酯交换反应的最佳催化剂。进一步对甲醇油摩尔比、反应温度、催化剂(NaOH)用量等工艺参数进行了优化。这些变量的最佳水平为甲醇与油的摩尔比为6:1,反应温度为60℃,催化剂的重量比为1%,可获得最大的生物柴油产量。从本研究的结果来看,哈密瓜籽油可以作为一种经济有效的原料成功地用于生产生物柴油。
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引用次数: 0
EFFECT OF SPACING ON REPRODUCTIVE PERFORMANCE OF INDIAN PEAFOWL (Pavo cristatus) IN CAPTIVITY 圈养条件下间距对印度孔雀繁殖性能的影响
Pub Date : 2023-01-05 DOI: 10.57041/pjs.v68i4.227
Z. Ali
Effective use of available cage spaces in wild bird breeding systems in captivity is ofprime economic concern. Present study was carried out at Punjab Wildlife Research InstituteFaisalabad to evaluate effect of cage sizes on reproductive performance of the Indian Peafowl (Pavo cristatus). Three groups; A, B and C of Indian Peafowl were selected and nurtured under threedifferent cage sizes, viz. 30, 21 and 12 square meters with an available space of 10, 7 and 4 square meters per bird, respectively. The birds were kept with a sex ratio of 1 male:2 female. Number of male mounting, egg production (average number of eggs laid per hen), average egg weight, percent egghatchability, percent egg fertility and chicks’ average weight were found significantly higher (P˂0.01)in Indian Peafowl reared in 21m2 cage size, followed by cage sizes of 30, and12 m2 respectively. Thisstudy indicated that, in case of Indian Peafowl, the breeding flock kept at intermediate cage spacingperformed better than that kept at large spacing or at too narrow spacing.
有效利用圈养野生鸟类繁殖系统中可用的笼空间是主要的经济问题。本研究是在旁遮普野生动物研究所进行的,目的是评估笼大小对印度孔雀(Pavo cristatus)繁殖性能的影响。三组;选取印度孔雀A、B、C三种不同尺寸的笼子进行饲养,分别为30、21和12平方米,每只可用空间分别为10、7和4平方米。这些鸟的性别比例为1雄2母。21m2、30 m2和12 m2笼尺寸饲养的印度孔雀,其雄蛋数、产蛋量(平均每只母鸡产蛋数)、平均蛋重、蛋可孵率、蛋受精率和雏鸡平均体重显著高于对照组(P小于0.01)。本研究表明,以印度孔雀为例,中等笼距饲养的繁殖群比大笼距或过窄笼距饲养的繁殖群表现更好。
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引用次数: 1
SELECTION OF DISCRIMINATIVE FEATURES FOR ARABIC PHONEME’S MISPRONUNCIATION DETECTION 阿拉伯语音素错读检测的判别特征选择
Pub Date : 2023-01-05 DOI: 10.57041/pjs.v67i4.606
M.J. Khan
Pronunciation training is an important part of Computer Assisted Pronunciation Training (CAPT) systems. Mispronunciation detection systems recognized pronunciation mistakes from user’s speech and provided them feedback about their pronunciation. Acoustic phonetic features plays a vital role in speech classification based applications. This research work investigated the suitability of various acoustic features: pitch, energy, spectrum flux, zero-crossing, Entropy and MelFrequency Cepstral Coefficients (MFCCs). Sequential Forward Selection (SFS) was used to find out most suitable acoustic features from the computed feature set. This study used K-Nearest Neighbors (K-NN) classifier was used to detect the pronunciation mistakes from Arabic phonemes. This research selected the set of most discriminative acoustic features for each phoneme. K-NN achieved accuracy of 92.15% for mispronunciation detection of Arabic Phonemes.
语音训练是计算机辅助语音训练系统的重要组成部分。发音错误检测系统从用户的语音中识别发音错误,并为用户提供发音反馈。声学语音特征在基于语音分类的应用中起着至关重要的作用。本研究考察了各种声学特征的适用性:基音、能量、频谱通量、过零、熵和MelFrequency Cepstral系数(MFCCs)。使用顺序前向选择(SFS)从计算的特征集中找出最合适的声学特征。本研究使用k -近邻分类器(K-NN)检测阿拉伯文音素的发音错误。本研究为每个音素选择了一组最具区别性的声学特征。K-NN对阿拉伯音素的误发音检测准确率达到92.15%。
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引用次数: 1
期刊
Pakistan journal of science
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