Pesticide Biochemistry and Physiology最新文献_第6页

N-acetylglucosamine sensor, Ngs1 contributes to Beauveria bassiana vegetative growth, oxidative phosphorylation, fungal development, and cell wall integrity during entomopathogen-insect interaction

IF 4.2 1区农林科学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Pesticide Biochemistry and Physiology

Pub Date : 2024-12-24 DOI: 10.1016/j.pestbp.2024.106273

Zhou Cui , Wu-Wei-Jie Yang , Zhi-Hao Yang , Long-Bin Zhang , Yi Guan

N-acetylglucosamine (GlcNAc), a key component of fungal cell walls and insect cuticles, is an important signal to activate fungal response during entomopathogen-insect interactions. Research on Ngs1, the only identified GlcNAc sensor and transducer, has been primarily restricted to Candida species. Although our previous work identified an Ngs1 homology in Beauveria bassiana, its physiological functions in entomopathogenic fungi remain largely unexplored. In this study, we unveiled the sub-localization of Ngs1 in the nucleolus. Further transcriptomic analysis revealed that Ngs1 plays a crucial role in vegetative growth, fungal development, and cell-wall construction by acting as a transcriptional mediator, particularly influencing carbon metabolism in response to insect cuticle stimulation. The absence of Ngs1 compromised vegetative growth across various carbon sources by downregulating expressions of key catalytic enzymes. Conversely, Ngs1 deficiency enhanced transcription levels of oxidative phosphorylation, leading to increased ATP and reactive oxygen species (ROS) production. Despite higher ATP levels, Ngs1-deletion mutants exhibited reduced asexual development and hyphal germination, primarily due to the function of Ngs1 in the central developmental pathway and Brg1/Nrg1-dependent pathway. Additionally, the downregulation of N-glycan biosynthesis in ΔNgs1 diminished cell wall components, resulting in decreased cell wall resistance to lysis and impaired fungal development. These findings advance our understanding of the regulatory role of Ngs1 in B. bassiana during host interactions and provide a theoretical foundation for engineering fungi to maintain or even enhance pesticidal activity.

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引用次数: 0

A UDP-glucuronosyltransferase gene UGT379A1 involved in detoxification of lufenuron in Diaphorina citri

IF 4.2 1区农林科学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Pesticide Biochemistry and Physiology

Pub Date : 2024-12-24 DOI: 10.1016/j.pestbp.2024.106260

Ze-Hao Huang , Qi Pan , Zhen Wu , Yu-Chao Shen , Si-Chen Li , Qi-Qi Yang , Shao-Hui Zhang , Shuang Lei , Yang-Yang Cui , Li-Li Ding , Shi-Jiang Yu , Liu Liu , Lin Cong , Bing-Hai Lou , Xue-Feng Wang , Chun Ran , Ling Liao

The Asian citrus psyllid, Diaphorina citri Kuwayama (Hemiptera: Psyllidae), is a devastating bacterial disease of commercial citrus. Presently, uridine diphosphate (UDP)-glycosyltransferases (UGTs), have been linked to the detoxification of pesticides, were known as phase II enzymes in the detoxification process. However, the role of UGTs in detoxification of lufenuron in Diaphorina citri is unknown. In this study, we identified a UGT gene, UGT379A1, which was significantly up-regulated under the exposure of lufenuron. The knockdown of UGT379A1 increased the susceptibility of D. citri to lufenuron. In vitro metabolism and Dixon plot analysis indicated that UGT379A1 could deplete lufenuron by sequestration. These results showed that UGT379A1 was involved in the lufenuron detoxification, which provides a theoretical basis for the prevention and control of D. citri.

{"title":"A UDP-glucuronosyltransferase gene UGT379A1 involved in detoxification of lufenuron in Diaphorina citri","authors":"Ze-Hao Huang , Qi Pan , Zhen Wu , Yu-Chao Shen , Si-Chen Li , Qi-Qi Yang , Shao-Hui Zhang , Shuang Lei , Yang-Yang Cui , Li-Li Ding , Shi-Jiang Yu , Liu Liu , Lin Cong , Bing-Hai Lou , Xue-Feng Wang , Chun Ran , Ling Liao","doi":"10.1016/j.pestbp.2024.106260","DOIUrl":"10.1016/j.pestbp.2024.106260","url":null,"abstract":"<div><div>The Asian citrus psyllid, <em>Diaphorina citri</em> Kuwayama (Hemiptera: Psyllidae), is a devastating bacterial disease of commercial citrus. Presently, uridine diphosphate (UDP)-glycosyltransferases (UGTs), have been linked to the detoxification of pesticides, were known as phase II enzymes in the detoxification process. However, the role of UGTs in detoxification of lufenuron in <em>Diaphorina citri</em> is unknown. In this study, we identified a UGT gene, <em>UGT379A1</em>, which was significantly up-regulated under the exposure of lufenuron. The knockdown of <em>UGT379A1</em> increased the susceptibility of <em>D. citri</em> to lufenuron. <em>In vitro</em> metabolism and Dixon plot analysis indicated that <em>UGT379A1</em> could deplete lufenuron by sequestration. These results showed that <em>UGT379A1</em> was involved in the lufenuron detoxification, which provides a theoretical basis for the prevention and control of <em>D. citri</em>.</div></div>","PeriodicalId":19828,"journal":{"name":"Pesticide Biochemistry and Physiology","volume":"208 ","pages":"Article 106260"},"PeriodicalIF":4.2,"publicationDate":"2024-12-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143099735","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A streamlined cloning and toxicity evaluation strategy of toxin-antitoxin systems in insect cells

IF 4.2 1区农林科学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Pesticide Biochemistry and Physiology

Pub Date : 2024-12-24 DOI: 10.1016/j.pestbp.2024.106261

Yunfei Li, Shizhe Hu, Jing Wang, Liping Lv, Xueting Zhao, Hanbing Li, Weihua Ma, Hongxia Hua, Zhihui Zhu

Toxin-antitoxin (TA) systems, which are prevalent in bacteria and archaea, have significant potential applications in eukaryotic cells due to their characteristic ability to inhibit cell growth. In particular, type II TA systems, where both the toxin and antitoxin are proteins, can serve as lethal and rescue genes in genetic pest management. However, traditional methods of cloning these systems often encounter difficulties due to cryptic promoter driven expression of toxins during the cloning process. To address this issue, we have developed a novel cloning method that incorporates an additional prokaryotic promoter to express the antitoxin, thus preventing the deleterious effects of toxin expression in bacterial cells. This method allows for efficient and straightforward construction of toxin vectors and the rapid screening of effective TA systems in insect cells. Our study focused on evaluating the toxicity of various type II TA systems in Sf9 insect cells. We cloned and tested toxins and antitoxins from Escherichia coli, Streptococcus pneumoniae, and Rickettsia rickettsii. The toxicity of each system was assessed by the number of red fluorescent cells post-transfection. Our results demonstrated that toxins such as MazF (E. coli-2782), RelE (Spn-1223), and RelE (Spn-1104) exhibited high levels of toxicity in Sf9 cells, while other toxins showed varying degrees of effectiveness. The corresponding antitoxins were able to neutralize the toxins in a dose-dependent manner, although some antitoxins, like MnT (R. felis-0357) and MazE (E. coli-4224), were ineffective in insect cells. This study highlights the potential for applying type II TA systems in genetic pest management and other eukaryotic applications. Our novel cloning approach provides a robust platform for the rapid and efficient evaluation of TA systems, paving the way for future research and application in pest control and beyond.

{"title":"A streamlined cloning and toxicity evaluation strategy of toxin-antitoxin systems in insect cells","authors":"Yunfei Li, Shizhe Hu, Jing Wang, Liping Lv, Xueting Zhao, Hanbing Li, Weihua Ma, Hongxia Hua, Zhihui Zhu","doi":"10.1016/j.pestbp.2024.106261","DOIUrl":"10.1016/j.pestbp.2024.106261","url":null,"abstract":"<div><div>Toxin-antitoxin (TA) systems, which are prevalent in bacteria and archaea, have significant potential applications in eukaryotic cells due to their characteristic ability to inhibit cell growth. In particular, type II TA systems, where both the toxin and antitoxin are proteins, can serve as lethal and rescue genes in genetic pest management. However, traditional methods of cloning these systems often encounter difficulties due to cryptic promoter driven expression of toxins during the cloning process. To address this issue, we have developed a novel cloning method that incorporates an additional prokaryotic promoter to express the antitoxin, thus preventing the deleterious effects of toxin expression in bacterial cells. This method allows for efficient and straightforward construction of toxin vectors and the rapid screening of effective TA systems in insect cells. Our study focused on evaluating the toxicity of various type II TA systems in Sf9 insect cells. We cloned and tested toxins and antitoxins from <em>Escherichia coli</em>, <em>Streptococcus pneumoniae</em>, and <em>Rickettsia rickettsii</em>. The toxicity of each system was assessed by the number of red fluorescent cells post-transfection. Our results demonstrated that toxins such as MazF (<em>E. coli</em>-2782), RelE (<em>Spn</em>-1223), and RelE (<em>Spn</em>-1104) exhibited high levels of toxicity in Sf9 cells, while other toxins showed varying degrees of effectiveness. The corresponding antitoxins were able to neutralize the toxins in a dose-dependent manner, although some antitoxins, like MnT (<em>R. felis</em>-0357) and MazE (<em>E. coli</em>-4224), were ineffective in insect cells. This study highlights the potential for applying type II TA systems in genetic pest management and other eukaryotic applications. Our novel cloning approach provides a robust platform for the rapid and efficient evaluation of TA systems, paving the way for future research and application in pest control and beyond.</div></div>","PeriodicalId":19828,"journal":{"name":"Pesticide Biochemistry and Physiology","volume":"208 ","pages":"Article 106261"},"PeriodicalIF":4.2,"publicationDate":"2024-12-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143156003","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identification of a new lineage of pheromone receptors in mirid bugs (Heteroptera: Miridae)

IF 4.2 1区农林科学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Pesticide Biochemistry and Physiology

Pub Date : 2024-12-24 DOI: 10.1016/j.pestbp.2024.106277

Sai Zhang , Shuwei Yan , Xiangdong Mei , Guirong Wang , Yang Liu

Sex pheromones, typically released by females are crucial signals for the reductive biology of insects, primarily detected by sex pheromone receptors (PRs). A clade of PRs in three mirid bugs, Apolygus lucorum, Adelphocoris lineolatus, and Adelphocoris suturalis, has been found to respond to pheromones, (E)-2-hexenyl butyrate (E2HB) and hexyl butyrate (HB), with higher sensitivity to E2HB. In this study, we aimed to identify PRs responsible for the other two pheromone components, HB and (E)-4-oxo-2-hexenal (4-OHE), by using a combination of phylogenetic analyses, sequence similarity analyses, and in vitro functional studies. As a result, five new candidate PRs (AlucOR34, AlinOR9, AlinOR10, AsutOR9, and AsutOR10) positioned outside of the previously known PR clade were identified. All five PRs were found to respond to both E2HB and HB, with some PRs exhibiting a significant and sensitive binding to HB. However, PRs for 4-OHE remains unidentified. Overall, our study suggests that mirid bugs have evolved two distinct lineages of PRs with similar response profiles. This research offers valuable insights into sex pheromone recognition within the peripheral olfactory system and contributes to the identification of PRs in mirid bugs, providing new targets for developing the behavioral regulators for these insects.

{"title":"Identification of a new lineage of pheromone receptors in mirid bugs (Heteroptera: Miridae)","authors":"Sai Zhang , Shuwei Yan , Xiangdong Mei , Guirong Wang , Yang Liu","doi":"10.1016/j.pestbp.2024.106277","DOIUrl":"10.1016/j.pestbp.2024.106277","url":null,"abstract":"<div><div>Sex pheromones, typically released by females are crucial signals for the reductive biology of insects, primarily detected by sex pheromone receptors (PRs). A clade of PRs in three mirid bugs, <em>Apolygus lucorum</em>, <em>Adelphocoris lineolatus</em>, and <em>Adelphocoris suturalis</em>, has been found to respond to pheromones, (E)-2-hexenyl butyrate (E2HB) and hexyl butyrate (HB), with higher sensitivity to E2HB. In this study, we aimed to identify PRs responsible for the other two pheromone components, HB and (E)-4-oxo-2-hexenal (4-OHE), by using a combination of phylogenetic analyses, sequence similarity analyses, and <em>in vitro</em> functional studies. As a result, five new candidate PRs (<em>AlucOR34</em>, <em>AlinOR9</em>, <em>AlinOR10</em>, <em>AsutOR9</em>, and <em>AsutOR10</em>) positioned outside of the previously known PR clade were identified. All five PRs were found to respond to both E2HB and HB, with some PRs exhibiting a significant and sensitive binding to HB. However, PRs for 4-OHE remains unidentified. Overall, our study suggests that mirid bugs have evolved two distinct lineages of PRs with similar response profiles. This research offers valuable insights into sex pheromone recognition within the peripheral olfactory system and contributes to the identification of PRs in mirid bugs, providing new targets for developing the behavioral regulators for these insects.</div></div>","PeriodicalId":19828,"journal":{"name":"Pesticide Biochemistry and Physiology","volume":"208 ","pages":"Article 106277"},"PeriodicalIF":4.2,"publicationDate":"2024-12-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143155708","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Phytochemicals in pomegranate peel extract attenuate oxidative damage in rats exposed to fipronil

IF 4.2 1区农林科学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Pesticide Biochemistry and Physiology

Pub Date : 2024-12-22 DOI: 10.1016/j.pestbp.2024.106275

Awad R. Abd Elfatah , Marwa E. Hassan , Yasmin E. Abdel-Mobdy , Osama M. Abd El fatah , Emam A. Abdelrahim , Mosaad A. Abdel-Wahhab

Fipronil (FPN), a pesticide frequently used in veterinary medicine and agriculture, has been linked to adverse health consequences in non-target creatures. This study was conducted to determine the phytochemical and vitamin content of pomegranate peel ethanolic extract (PPE) and to assess its hepatoprotective impact in rats exposed to FPN insecticide. The phytochemicals were tested using LC-MS/MS, whilst the vitamins were evaluated using HPLC. Six groups of male albino rats were treated for three months, including the control group and those treated with PPE (400 mg/kg b.w.), FPN at low dosage (16.16 mg/kg b.w.), or high dose (48.5 mg/kg b.w.) alone or in combination with PPE. Blood and tissue samples were taken for various hematological, biochemical, and histopathological analyses. HPLC results revealed that PPE contains water and oil-soluble vitamins; also, LC-MS/MS identified 21 chemicals belonging to tannins, steroids, tannins, flavonoids, phenolics, alkaloids, terpenoids, and saponins. The biological study revealed that FPN caused dose-dependent changes in RBCs, WBCs, MCHC, PLT count, MCH, MCV, Hb, HCT, ALT, AST, ALP, Alb, TP, urea, uric acid, creatinine, Cho, Tri G, HDL, LDL, CAT, SOD, MDA, GST, testosterone levels, and histological changes in liver and kidney. PPE administration did not show any significant changes in all the tested parameters. Co-administration with FPN and PPE induced a significant improvement in all the tested parameters towards the control levels, owing to its strong antioxidant activity. Consequently, PPE should be considered a dietary supplement in areas with high levels of FPN exposure.

{"title":"Phytochemicals in pomegranate peel extract attenuate oxidative damage in rats exposed to fipronil","authors":"Awad R. Abd Elfatah , Marwa E. Hassan , Yasmin E. Abdel-Mobdy , Osama M. Abd El fatah , Emam A. Abdelrahim , Mosaad A. Abdel-Wahhab","doi":"10.1016/j.pestbp.2024.106275","DOIUrl":"10.1016/j.pestbp.2024.106275","url":null,"abstract":"<div><div>Fipronil (FPN), a pesticide frequently used in veterinary medicine and agriculture, has been linked to adverse health consequences in non-target creatures. This study was conducted to determine the phytochemical and vitamin content of pomegranate peel ethanolic extract (PPE) and to assess its hepatoprotective impact in rats exposed to FPN insecticide. The phytochemicals were tested using LC-MS/MS, whilst the vitamins were evaluated using HPLC. Six groups of male albino rats were treated for three months, including the control group and those treated with PPE (400 mg/kg b.w.), FPN at low dosage (16.16 mg/kg b.w.), or high dose (48.5 mg/kg b.w.) alone or in combination with PPE. Blood and tissue samples were taken for various hematological, biochemical, and histopathological analyses. HPLC results revealed that PPE contains water and oil-soluble vitamins; also, LC-MS/MS identified 21 chemicals belonging to tannins, steroids, tannins, flavonoids, phenolics, alkaloids, terpenoids, and saponins. The biological study revealed that FPN caused dose-dependent changes in RBCs, WBCs, MCHC, PLT count, MCH, MCV, Hb, HCT, ALT, AST, ALP, Alb, TP, urea, uric acid, creatinine, Cho, Tri G, HDL, LDL, CAT, SOD, MDA, GST, testosterone levels, and histological changes in liver and kidney. PPE administration did not show any significant changes in all the tested parameters. Co-administration with FPN and PPE induced a significant improvement in all the tested parameters towards the control levels, owing to its strong antioxidant activity. Consequently, PPE should be considered a dietary supplement in areas with high levels of FPN exposure.</div></div>","PeriodicalId":19828,"journal":{"name":"Pesticide Biochemistry and Physiology","volume":"208 ","pages":"Article 106275"},"PeriodicalIF":4.2,"publicationDate":"2024-12-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143099731","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Influence of triflumezopyrim and triadimefon co-exposure on enzymatic activity and gene expression changes in honey bees (Apis mellifera L.)

IF 4.2 1区农林科学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Pesticide Biochemistry and Physiology

Pub Date : 2024-12-22 DOI: 10.1016/j.pestbp.2024.106269

Xuan Liu , Chunxiao Wang , Wenchao Zhu , Lu Lv , Xuejing Wang , Yanhua Wang , Zhixin Wang , Xiaojun Gai

Pollination insects frequently encounter complex mixtures of pesticides within agricultural ecosystems. However, current risk assessments for pesticides focus primarily on single agents, failing to reflect real-world conditions. Mesoionic insecticide triflumezopyrim (TFM) and triazole fungicide triadimefon (TAD) are two compounds often detected together in the environment, raising concerns over their combined toxic effects on pollinators. In this context, our study aimed to explore the enzymatic and transcriptional responses in the honey bee (Apis mellifera L.) when exposed to a mixture of TFM and TAD. Our findings revealed that co-exposure to these two pesticides induced acute synergistic toxicity in A. mellifera. Furthermore, significant alterations were observed in the levels of MDA, AChE, GST, and trypsin, along with the expression of four genes (abaecin, CRBXase, CYP6AS14, and CYP306A1) linked to oxidative stress, neural function, detoxification pathways, digestion, and immune competence. Additionally, both pesticides were found to modify the molecular conformation of CAT and AChE, thereby influencing their enzymatic activities. These results underscored the biochemical and molecular toxicities resulting from the combined action of TFM and TAD on A. mellifera, offering critical insights into the ecological impact of pesticide mixtures on pollinators. Importantly, the co-presence of TFM and TAD might exacerbate physiological damage in A. mellifera, likely due to their interactive effects. Collectively, this study represented a substantial advancement in comprehending the toxicological impacts of commonly used agricultural pesticides and provided valuable foundations for developing effective strategies to mitigate their harmful effects on pollination insects.

{"title":"Influence of triflumezopyrim and triadimefon co-exposure on enzymatic activity and gene expression changes in honey bees (Apis mellifera L.)","authors":"Xuan Liu , Chunxiao Wang , Wenchao Zhu , Lu Lv , Xuejing Wang , Yanhua Wang , Zhixin Wang , Xiaojun Gai","doi":"10.1016/j.pestbp.2024.106269","DOIUrl":"10.1016/j.pestbp.2024.106269","url":null,"abstract":"<div><div>Pollination insects frequently encounter complex mixtures of pesticides within agricultural ecosystems. However, current risk assessments for pesticides focus primarily on single agents, failing to reflect real-world conditions. Mesoionic insecticide triflumezopyrim (TFM) and triazole fungicide triadimefon (TAD) are two compounds often detected together in the environment, raising concerns over their combined toxic effects on pollinators. In this context, our study aimed to explore the enzymatic and transcriptional responses in the honey bee (<em>Apis mellifera</em> L.) when exposed to a mixture of TFM and TAD. Our findings revealed that co-exposure to these two pesticides induced acute synergistic toxicity in <em>A. mellifera</em>. Furthermore, significant alterations were observed in the levels of MDA, AChE, GST, and trypsin, along with the expression of four genes (<em>abaecin</em>, <em>CRBXase</em>, <em>CYP6AS14</em>, and <em>CYP306A1</em>) linked to oxidative stress, neural function, detoxification pathways, digestion, and immune competence. Additionally, both pesticides were found to modify the molecular conformation of CAT and AChE, thereby influencing their enzymatic activities. These results underscored the biochemical and molecular toxicities resulting from the combined action of TFM and TAD on <em>A. mellifera</em>, offering critical insights into the ecological impact of pesticide mixtures on pollinators. Importantly, the co-presence of TFM and TAD might exacerbate physiological damage in <em>A. mellifera</em>, likely due to their interactive effects. Collectively, this study represented a substantial advancement in comprehending the toxicological impacts of commonly used agricultural pesticides and provided valuable foundations for developing effective strategies to mitigate their harmful effects on pollination insects.</div></div>","PeriodicalId":19828,"journal":{"name":"Pesticide Biochemistry and Physiology","volume":"208 ","pages":"Article 106269"},"PeriodicalIF":4.2,"publicationDate":"2024-12-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143156004","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

In-depth physiological study on the sustainable application of a botanical insecticide with low mammalian toxicity against Aedes aegypti (Diptera: Culicidae)

IF 4.2 1区农林科学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Pesticide Biochemistry and Physiology

Pub Date : 2024-12-22 DOI: 10.1016/j.pestbp.2024.106270

Zenghua Xing , Yaya Liu , Jing Sun , Yulin Gan , Erkang Liu , Xizhong Yan , Chi Hao , Li Ma , Xingtao Qie

Research into the exploration of bioactive insecticides as an alternative to synthetic compounds has garnered increasing attention, particularly in the context of sustainable pest management. In this study, a two-sex life table was constructed based on the Waco strain to determine whether the prolonged larval period in haedoxan A-resistant (HAR) strain Aedes aegypti is related to ecdysone-regulated development. The effect of ecdysone on the sensitivity of the 3rd-instar larvae to HA was investigated, along with the determination of 20-hydroxyecdysone (20E) content in A. aegypti. The results showed that the 20E content in larvae from HAR strain was significantly lower than that of Waco strain. And, the sensitivity of larvae treated with ecdysone to HA was increased. Two-sex life table results indicated that ecdysone can accelerate the growth and development of larvae, however, it also shortened the life span of adult mosquitoes and reduced reproduction rate. Notably, cytotoxic activity against human normal liver cell L02 demonstrated that HA was less toxic and did not cause intracellular oxidative stress compared to permethrin. Overall, these findings contribute to a deeper understanding of the mechanisms by which the larval period of A. aegypti is prolonged under HA selection. Furthermore, our results provide valuable insights for the application of HA as an effective botanical insecticide.

{"title":"In-depth physiological study on the sustainable application of a botanical insecticide with low mammalian toxicity against Aedes aegypti (Diptera: Culicidae)","authors":"Zenghua Xing , Yaya Liu , Jing Sun , Yulin Gan , Erkang Liu , Xizhong Yan , Chi Hao , Li Ma , Xingtao Qie","doi":"10.1016/j.pestbp.2024.106270","DOIUrl":"10.1016/j.pestbp.2024.106270","url":null,"abstract":"<div><div>Research into the exploration of bioactive insecticides as an alternative to synthetic compounds has garnered increasing attention, particularly in the context of sustainable pest management. In this study, a two-sex life table was constructed based on the Waco strain to determine whether the prolonged larval period in haedoxan A-resistant (HAR) strain <em>Aedes aegypti</em> is related to ecdysone-regulated development. The effect of ecdysone on the sensitivity of the 3rd-instar larvae to HA was investigated, along with the determination of 20-hydroxyecdysone (20E) content in <em>A. aegypti</em>. The results showed that the 20E content in larvae from HAR strain was significantly lower than that of Waco strain. And, the sensitivity of larvae treated with ecdysone to HA was increased. Two-sex life table results indicated that ecdysone can accelerate the growth and development of larvae, however, it also shortened the life span of adult mosquitoes and reduced reproduction rate. Notably, cytotoxic activity against human normal liver cell L02 demonstrated that HA was less toxic and did not cause intracellular oxidative stress compared to permethrin. Overall, these findings contribute to a deeper understanding of the mechanisms by which the larval period of <em>A. aegypti</em> is prolonged under HA selection. Furthermore, our results provide valuable insights for the application of HA as an effective botanical insecticide.</div></div>","PeriodicalId":19828,"journal":{"name":"Pesticide Biochemistry and Physiology","volume":"208 ","pages":"Article 106270"},"PeriodicalIF":4.2,"publicationDate":"2024-12-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143155710","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

miR-571 manipulating termite immune response to fungus and showing potential for green management of Copotermes formosanus (Blattodea: Isoptera)

IF 4.2 1区农林科学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Pesticide Biochemistry and Physiology

Pub Date : 2024-12-22 DOI: 10.1016/j.pestbp.2024.106274

Weiwen Chen, Zhiqiang Li

Termites are not merely social insects; they are also globally important insect pests. MicroRNAs (miRNAs) are potential molecular targets for the biological control of termites. However, their role in termite resistance to pathogens, particularly their impact on termite social immune behaviour, remains unclear. In this study, we identified 50 differentially expressed miRNAs in Coptotermes formosanus, a globally economically important termite pest, in response to Metarhizium anisopliae infection. Injecting miR-571 agomir, one of significantly upregulated miRNAs, significantly increased termite mortality without or with M. anisopliae infection (compared to that with M. anisopliae infection alone). Meanwhile, termites infected with M. anisopliae exhibited a significant reduction in the avoidance, trophallaxis, and grooming behaviors. Subsequently, we identified POP5 as a target gene of miR-571 and found that miR-571-POP5 inhibits the termite immune response to M. anisopliae by inhibiting the expression of downstream genes, trypsin-like serine protease and serine protease. Finally, we confirmed that the ingestion of miR-571 agomir also increased the mortality of M. anisopliae-infected termites. Our findings enhance knowledge regarding miRNA role in insect social immunity, pathogen manipulation mechanisms, and optimizing pathogen effectiveness through insect miRNAs. This offers new molecular targets for the biological control of termites.

{"title":"miR-571 manipulating termite immune response to fungus and showing potential for green management of Copotermes formosanus (Blattodea: Isoptera)","authors":"Weiwen Chen, Zhiqiang Li","doi":"10.1016/j.pestbp.2024.106274","DOIUrl":"10.1016/j.pestbp.2024.106274","url":null,"abstract":"<div><div>Termites are not merely social insects; they are also globally important insect pests. MicroRNAs (miRNAs) are potential molecular targets for the biological control of termites. However, their role in termite resistance to pathogens, particularly their impact on termite social immune behaviour, remains unclear. In this study, we identified 50 differentially expressed miRNAs in <em>Coptotermes formosanus</em>, a globally economically important termite pest, in response to <em>Metarhizium anisopliae</em> infection. Injecting miR-571 agomir, one of significantly upregulated miRNAs, significantly increased termite mortality without or with <em>M. anisopliae</em> infection (compared to that with <em>M. anisopliae</em> infection alone). Meanwhile, termites infected with <em>M. anisopliae</em> exhibited a significant reduction in the avoidance, trophallaxis, and grooming behaviors. Subsequently, we identified <em>POP5</em> as a target gene of miR-571 and found that miR-571-POP5 inhibits the termite immune response to <em>M. anisopliae</em> by inhibiting the expression of downstream genes, trypsin-like serine protease and serine protease. Finally, we confirmed that the ingestion of miR-571 agomir also increased the mortality of <em>M. anisopliae</em>-infected termites. Our findings enhance knowledge regarding miRNA role in insect social immunity, pathogen manipulation mechanisms, and optimizing pathogen effectiveness through insect miRNAs. This offers new molecular targets for the biological control of termites.</div></div>","PeriodicalId":19828,"journal":{"name":"Pesticide Biochemistry and Physiology","volume":"208 ","pages":"Article 106274"},"PeriodicalIF":4.2,"publicationDate":"2024-12-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143099732","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The diverse enzymatic targets of the essential oils of Ilex purpurea and Cymbopogon martini and the major components potentially mitigated the resistance development in tick Haemaphysalis longicornis

IF 4.2 1区农林科学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Pesticide Biochemistry and Physiology

Pub Date : 2024-12-22 DOI: 10.1016/j.pestbp.2024.106271

Zhihua Gao, Lingqian Bai, Xiaofeng Xu, Bingxia Feng, Ruya Cao, Weikang Zhao, Jiayi Zhang, Weijia Xing, Xiaolong Yang

The long-term use of chemical acaricides not only pollutes the environment, but also easily causes tick resistance. Plant essential oils, a kind of secondary metabolites in plants, are an important way to develop new modern pesticides. The study analyzed the acaricidal activity and molecular mechanisms of Ilex purpurea EO and Cymbopogon martini EO on Haemaphysalis longicornis (Acari: Ixodidae) unfed ticks. The chemical analysis (GC–MS) identified 7 components in I. purpurea EO and 4 in C. martini EO. The effects of the oils on unfed larvae of H. longicornis were assessed by larval packet test. The results revealed that I. purpurea EO and C. martini EO had significant toxicity against the unfed larvae. The present work evaluated the toxicity of EOs and methyl salicylate against unfed nymphs and adults of H. longicornis by immersion test. The results showed that I. purpurea EO, C. martini EO and methyl salicylate had significant toxicity against the unfed nymphs and adults. Enzyme assays showed that the oils and methyl salicylate significantly inhibited AChE and CarE activity, I. purpurea EO significantly inhibited CAT activity. These results revealed that the diverse enzymatic targets of I. purpurea EO, C. martini EO and the major components of I. purpurea potentially mitigated the resistance development in tick H. longicornis. This study revealed the mechanisms of action of I. purpurea EO and C. martini EO in nature and biotechnology. These results will open new directions for understanding how EOs interfere with tick biological systems and has broad implications for the field of acaricide design.

{"title":"The diverse enzymatic targets of the essential oils of Ilex purpurea and Cymbopogon martini and the major components potentially mitigated the resistance development in tick Haemaphysalis longicornis","authors":"Zhihua Gao, Lingqian Bai, Xiaofeng Xu, Bingxia Feng, Ruya Cao, Weikang Zhao, Jiayi Zhang, Weijia Xing, Xiaolong Yang","doi":"10.1016/j.pestbp.2024.106271","DOIUrl":"10.1016/j.pestbp.2024.106271","url":null,"abstract":"<div><div>The long-term use of chemical acaricides not only pollutes the environment, but also easily causes tick resistance. Plant essential oils, a kind of secondary metabolites in plants, are an important way to develop new modern pesticides. The study analyzed the acaricidal activity and molecular mechanisms of <em>Ilex purpurea</em> EO and <em>Cymbopogon martini</em> EO on <em>Haemaphysalis longicornis</em> (Acari: Ixodidae) unfed ticks. The chemical analysis (GC–MS) identified 7 components in <em>I. purpurea</em> EO and 4 in <em>C. martini</em> EO. The effects of the oils on unfed larvae of <em>H. longicornis</em> were assessed by larval packet test. The results revealed that <em>I. purpurea</em> EO and <em>C. martini</em> EO had significant toxicity against the unfed larvae. The present work evaluated the toxicity of EOs and methyl salicylate against unfed nymphs and adults of <em>H. longicornis</em> by immersion test. The results showed that <em>I. purpurea</em> EO, <em>C. martini</em> EO and methyl salicylate had significant toxicity against the unfed nymphs and adults. Enzyme assays showed that the oils and methyl salicylate significantly inhibited AChE and CarE activity, <em>I. purpurea</em> EO significantly inhibited CAT activity. These results revealed that the diverse enzymatic targets of <em>I. purpurea</em> EO, <em>C. martini</em> EO and the major components of <em>I. purpurea</em> potentially mitigated the resistance development in tick <em>H. longicornis</em>. This study revealed the mechanisms of action of <em>I. purpurea</em> EO and <em>C. martini</em> EO in nature and biotechnology. These results will open new directions for understanding how EOs interfere with tick biological systems and has broad implications for the field of acaricide design.</div></div>","PeriodicalId":19828,"journal":{"name":"Pesticide Biochemistry and Physiology","volume":"208 ","pages":"Article 106271"},"PeriodicalIF":4.2,"publicationDate":"2024-12-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143099734","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cytochrome P450 CYP6EM1 confers resistance to thiamethoxam in the whitefly Bemisia tabaci (Hemiptera: Aleyrodidae) via detoxification metabolism

IF 4.2 1区农林科学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Pesticide Biochemistry and Physiology

Pub Date : 2024-12-22 DOI: 10.1016/j.pestbp.2024.106272

Mingjiao Huang , Peipan Gong , Cheng Yin , Jing Yang , Shaonan Liu , Buli Fu , Xuegao Wei , Jinjin Liang , Hu Xue , Chao He , Tianhua Du , Chao Wang , Yao Ji , JinYu Hu , Rong Zhang , Natalia A. Belyakova , Youjun Zhang , Xin Yang

The whitefly Bemisia tabaci (Hemiptera: Gennadius) is a notorious and highly polyphagous agricultural pest that is well known for its ability to transmit a wide range of serious plant pathogenic viruses. The field populations of B. tabaci in some areas have developed resistance to thiamethoxam. We found that high expression of CYP6EM1 can enhance the resistance of B. tabaci to dinotefuran. It is unclear whether CYP6EM1 is involved in the resistance of B. tabaci to the same neonicotinoid pesticide, thiamethoxam. The results of the present study demonstrated that the expression of CYP6EM1 could be induced within 9 h after the exposure of B. tabaci adults to thiamethoxam. Molecular docking analyses, with a binding energy of −6.13 cal/mol, revealed a strong binding affinity between thiamethoxam and the CYP6EM1 protein, implying that CYP6EM1 may be involved in thiamethoxam resistance. Compared with that in the susceptible strain, the mRNA expression level of the CYP6EM1 gene was significantly greater in thiamethoxam-resistant strains (R^#1, 9.93-fold, P = 0.0008; R^#2, 40.43-fold, P = 0.0013; R^#3, 27.40-fold, P = 0.0002; R^#4, 21.63-fold, P = 0.0003 and R^#5, 28.65-fold, P = 0.0006). Loss and gain of function studies in vivo were performed via RNA interference and transgenic expression in Drosophila melanogaster, and the results confirmed the role of CYP6EM1 in conferring such resistance. An in vitro metabolism assay revealed that CYP6EM1 directly metabolized 15.60 % of thiamethoxam. This study provides solid evidence for the critical role of CYP6EM1 in the metabolism of thiamethoxam, which contributes to resistance. Our work provides a deeper understanding of the mechanism underlying neonicotinoid resistance and contributes valuable insights for the sustainable management of global pests such as whiteflies.

{"title":"Cytochrome P450 CYP6EM1 confers resistance to thiamethoxam in the whitefly Bemisia tabaci (Hemiptera: Aleyrodidae) via detoxification metabolism","authors":"Mingjiao Huang , Peipan Gong , Cheng Yin , Jing Yang , Shaonan Liu , Buli Fu , Xuegao Wei , Jinjin Liang , Hu Xue , Chao He , Tianhua Du , Chao Wang , Yao Ji , JinYu Hu , Rong Zhang , Natalia A. Belyakova , Youjun Zhang , Xin Yang","doi":"10.1016/j.pestbp.2024.106272","DOIUrl":"10.1016/j.pestbp.2024.106272","url":null,"abstract":"<div><div>The whitefly <em>Bemisia tabaci</em> (Hemiptera: Gennadius) is a notorious and highly polyphagous agricultural pest that is well known for its ability to transmit a wide range of serious plant pathogenic viruses. The field populations of <em>B. tabaci</em> in some areas have developed resistance to thiamethoxam. We found that high expression of <em>CYP6EM1</em> can enhance the resistance of <em>B. tabaci</em> to dinotefuran. It is unclear whether <em>CYP6EM1</em> is involved in the resistance of <em>B. tabaci</em> to the same neonicotinoid pesticide, thiamethoxam. The results of the present study demonstrated that the expression of <em>CYP6EM1</em> could be induced within 9 h after the exposure of <em>B. tabaci</em> adults to thiamethoxam. Molecular docking analyses, with a binding energy of −6.13 cal/mol, revealed a strong binding affinity between thiamethoxam and the CYP6EM1 protein, implying that <em>CYP6EM1</em> may be involved in thiamethoxam resistance. Compared with that in the susceptible strain, the mRNA expression level of the <em>CYP6EM1</em> gene was significantly greater in thiamethoxam-resistant strains (R<sup>#1</sup>, 9.93-fold, <em>P</em> = 0.0008; R<sup>#2</sup>, 40.43-fold, <em>P</em> = 0.0013; R<sup>#3</sup>, 27.40-fold, <em>P</em> = 0.0002; R<sup>#4</sup>, 21.63-fold, <em>P</em> = 0.0003 and R<sup>#5</sup>, 28.65-fold, <em>P</em> = 0.0006). Loss and gain of function studies in vivo were performed via RNA interference and transgenic expression in <em>Drosophila melanogaster</em>, and the results confirmed the role of <em>CYP6EM1</em> in conferring such resistance. An in vitro metabolism assay revealed that <em>CYP6EM1</em> directly metabolized 15.60 % of thiamethoxam. This study provides solid evidence for the critical role of <em>CYP6EM1</em> in the metabolism of thiamethoxam, which contributes to resistance. Our work provides a deeper understanding of the mechanism underlying neonicotinoid resistance and contributes valuable insights for the sustainable management of global pests such as whiteflies.</div></div>","PeriodicalId":19828,"journal":{"name":"Pesticide Biochemistry and Physiology","volume":"208 ","pages":"Article 106272"},"PeriodicalIF":4.2,"publicationDate":"2024-12-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143095547","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0