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Integrated transcriptome and 1H NMR-based metabolome to explore the potential mechanism of Spodoptera litura in response to flupyrimin 整合转录组和基于 1H NMR 的代谢组,探究鞘翅目昆虫对氟嘧啶的潜在响应机制
IF 4.2 1区 农林科学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2024-09-24 DOI: 10.1016/j.pestbp.2024.106146
Xiao-Fang Yuan , Hong Zhong , Zhao-Ying Xia , Zhan-Jun Lu , Wei Chen , Ying-Xue Liu , Gu-Chun Zhou , Xiao-Qiang Liu , Ming-Jie Deng , Hai-Zhong Yu
Flupyrimin (FLP) is a novel class of insecticide acting on insect nicotinic acetylcholine receptor (nAChR) and shows robust insecticidal activity. However, the toxicological effects of FLP on Spodoptera litura have not been revealed. In this study, the results showed that the larval survival rate decreased significantly with increasing concentration of FLP. The hematoxylin-eosin (HE) staining showed that FLP exposure damages the structure of the larval midgut. Additionally, FLP treatments significantly increased the activities of detoxification (GST and CarE) and digestive (α-Amylase and Trypsin) enzymes and reduced lipase activity. Transcriptome sequencing identified 855, 1493 and 735 differentially expressed genes (DEGs) at 12 h, 24 h and 48 h after exposure to 3 mM FLP, respectively. Gene function enrichment analysis revealed that DEGs were mainly related to fatty acid metabolic, protein processing in the endoplasmic reticulum and drug metabolism-cytochrome P450. The DEGs associated with food digestion and detoxification was validated by reverse-transcription quantitative PCR (RT-qPCR). Furthermore, a total of fifteen energy-related metabolites were identified, among which thirteen metabolisms were significantly influenced after FLP treatment based on 1H NMR-based metabolome analysis, including tyrosine, glucose, trehalose, malate, threonine, proline, glycine, lysine, citrate, alanine, lactate, valine, and leucine. Taken together, these results provide useful information for revealing the toxicological effect of FLP against S. litura.
氟嘧啶(FLP)是一种作用于昆虫烟碱乙酰胆碱受体(nAChR)的新型杀虫剂,具有很强的杀虫活性。然而,FLP 对斑翅蝶类的毒理效应尚未揭示。本研究结果表明,随着 FLP 浓度的增加,幼虫存活率显著下降。苏木精-伊红(HE)染色表明,接触 FLP 会破坏幼虫中肠的结构。此外,FLP 处理还明显提高了解毒酶(GST 和 CarE)和消化酶(α-淀粉酶和胰蛋白酶)的活性,降低了脂肪酶的活性。转录组测序发现,暴露于 3 mM FLP 后 12 小时、24 小时和 48 小时分别有 855、1493 和 735 个差异表达基因(DEGs)。基因功能富集分析表明,差异表达基因主要与脂肪酸代谢、内质网蛋白质加工和药物代谢-细胞色素 P450 有关。反转录定量 PCR(RT-qPCR)验证了与食物消化和解毒相关的 DEGs。此外,根据基于 1H NMR 的代谢组分析,共鉴定出 15 种能量相关代谢物,其中 13 种代谢物在 FLP 处理后受到显著影响,包括酪氨酸、葡萄糖、三卤糖、苹果酸、苏氨酸、脯氨酸、甘氨酸、赖氨酸、柠檬酸、丙氨酸、乳酸、缬氨酸和亮氨酸。总之,这些结果为揭示 FLP 对 S. litura 的毒理作用提供了有用的信息。
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引用次数: 0
Flupyradifurone activates DUM neuron nicotinic acetylcholine receptors and stimulates an increase in intracellular calcium through the ryanodine receptors 氟吡拉呋酮可激活 DUM 神经元的烟碱乙酰胆碱受体,并通过雷诺丁受体刺激细胞内钙的增加
IF 4.2 1区 农林科学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2024-09-24 DOI: 10.1016/j.pestbp.2024.106147
Maria Taha , Alison Cartereau , Emiliane Taillebois , Steeve H. Thany
Insect neuronal nicotinic acetylcholine receptors (nAChRs) are transmembrane receptors that play a key role in the development and synaptic plasticity of both vertebrates and invertebrates, and are considered to be major targets of several insecticides. We used dorsal unpaired median (DUM) neurons, which are insect neurosecretory cells, to explore what type of nAChRs are involved in flupyradifurone's (FLU) mode of action, and to study the role of calcium release from intracellular stores in this process. Using whole-cell patch-clamp and fura-2-AM calcium imaging techniques, we found that inhibition of IP3Rs through application of 2-APB reduced FLU inward currents, but did not affect the intracellular calcium release induced by FLU. In contrast, inhibition of RyRs using ryanodine, led to reduction of intracellular calcium increase following FLU pulse application. These results suggested that FLU inward currents are likely due to a combination of the direct effects of FLU on DUM neuron nAChRs and the subsequent calcium release from RyRs.
昆虫神经元烟碱乙酰胆碱受体(nAChRs)是一种跨膜受体,在脊椎动物和无脊椎动物的发育和突触可塑性中起着关键作用,被认为是多种杀虫剂的主要靶标。我们利用昆虫的神经分泌细胞--背侧无对正中神经元(DUM)来探索哪种类型的nAChRs参与了氟哌啶酮(FLU)的作用模式,并研究钙从细胞内储存释放在这一过程中的作用。通过使用全细胞膜片钳和呋喃-2-AM 钙成像技术,我们发现通过应用 2-APB 抑制 IP3Rs 可降低 FLU 的内向电流,但不会影响 FLU 诱导的细胞内钙释放。相反,使用雷诺丁抑制 RyRs 可减少 FLU 脉冲应用后细胞内钙的增加。这些结果表明,FLU 内向电流可能是 FLU 对 DUM 神经元 nAChRs 的直接作用和 RyRs 随后的钙释放共同作用的结果。
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引用次数: 0
Phyto-toxicological effect of fipronil to plant seedlings: Assessing germination attributes, root-tip morphology, oxidative stress, and cellular respiration indices 氟虫腈对植物幼苗的植物毒理学影响:评估发芽属性、根尖形态、氧化应激和细胞呼吸指数
IF 4.2 1区 农林科学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2024-09-23 DOI: 10.1016/j.pestbp.2024.106135
Mohammad Shahid , Udai B. Singh , Mohammad Abul Farah , Khalid Mashay Al-Anazi
Pesticides including insecticides are applied in agricultural practices to control insect pests. However, their excessive usage often poses a severe threat to the growth, physiology, and biochemistry of plants. Here, responses of chickpea and greengram seedlings exposed to three fipronil (FIP) concentrations i. e. 100 (1×), 200 (2×) and 300 (3×) μg mL−1 was evaluated under in vitro. Among doses, 3× had a greater negative impact on germination attributes, root-shoot elongation, vigor indices, length ratios, and survival of seedlings. Besides, the morphological distortion in root tips, oxidative stress generation, and cellular death in fipronil-supplemented root seedlings were observed under scanning electron (SEM) and confocal laser scanning (CLSM), respectively. A significant (p ≤ 0.05) and pronounced upsurge in plant stressor metabolites such as proline, malondialdehyde (MDA), electrolyte leakage (EL), hydrogen peroxide (H2O2) content, and antioxidants enzymes in plant seedlings further confirmed the fipronil toxicity. In addition, a concentration-dependent decrease in respiration efficiency (RE) and ATP content in FIP-treated seedlings was observed. Reduced mitotic index (MI) and numerous chromosomal anomalies (CAs) in root meristematic cells of seedlings are a clear indication of insecticide-induced cytotoxicity. Furthermore, a dose-dependent increase in DNA damage in root meristematic cells of greengram revealed the genotoxic potential of fipronil. Conclusively, fipronil suggested phyto and cyto-genotoxic effects that emphasize their careful monitoring in soils before application and their optimum addition in soil-plant systems. It is high time to prepare both target-specific and slow-released agrochemical formulations for crop protection with concurrent safeguarding of the soil.
农业生产中使用杀虫剂来控制害虫。然而,过量使用杀虫剂往往会对植物的生长、生理和生化造成严重威胁。在此,我们对鹰嘴豆和油菜幼苗暴露于三种氟虫腈(FIP)浓度(即 100 (1×)、200 (2×) 和 300 (3×) μg mL-1)下的反应进行了离体评估。在各剂量中,3×对萌芽属性、根-芽伸长、活力指数、长度比和幼苗存活率的负面影响较大。此外,通过扫描电子显微镜(SEM)和激光共聚焦扫描(CLSM)分别观察到氟虫腈补药根苗的根尖形态扭曲、氧化应激产生和细胞死亡。植物幼苗中的脯氨酸、丙二醛(MDA)、电解质渗漏(EL)、过氧化氢(H2O2)含量和抗氧化酶等植物胁迫代谢物明显增加(p ≤ 0.05),进一步证实了氟虫腈的毒性。此外,还观察到氟虫腈处理的幼苗呼吸效率(RE)和 ATP 含量的下降与浓度有关。秧苗根部分生组织细胞有丝分裂指数(MI)的降低和大量染色体异常(CA)是杀虫剂诱导细胞毒性的明显迹象。此外,油菜根分生组织细胞中 DNA 损伤的剂量依赖性增加也揭示了氟虫腈的潜在遗传毒性。总之,氟虫腈具有植物和细胞遗传毒性作用,这就要求在施药前对土壤进行仔细监测,并在土壤-植物系统中进行最佳添加。在保护土壤的同时,配制针对特定目标的缓释农用化学品制剂来保护作物的时机已经成熟。
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引用次数: 0
Corrigendum to “Biological and molecular characterizations of field fludioxonil-resistant isolates of Fusarium graminearum” [Pesticide Biochemistry and Physiology 184 (2022) 105101] "禾谷镰刀菌田间氟啶虫酰胺抗性分离物的生物学和分子特征"[农药生物化学和生理学 184 (2022) 105101]更正
IF 4.2 1区 农林科学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2024-09-23 DOI: 10.1016/j.pestbp.2024.106144
Ziyue Wen, Jingrui Wang, Chen Jiao, Wenyong Shao, Zhonghua Ma
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引用次数: 0
Baseline susceptibility, risk assessment of resistance, changes in insecticides sensitivity and fitness after selection of dimpropyridaz in Aphis gossypii Glover (Hemiptera: Aphididae) 蚜虫 Glover(半翅目:蚜科)对二丙哒嗪的基线敏感性、抗药性风险评估、杀虫剂敏感性变化以及选择二丙哒嗪后的适应性
IF 4.2 1区 农林科学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2024-09-20 DOI: 10.1016/j.pestbp.2024.106138
Jiao Shang , Haishan Wang, Wenyang Dong, Xinyu Guo, Jiahao Zhu, Pei Liang, Xueyan Shi
Dimpropyridaz as a novel insecticide is registered for the management of many aphid species and other piercing-sucking insects. For the aim to clarify the susceptibility of natural population and the resistance risk of dimpropyridaz in Aphis gossypii in China, the baseline susceptibility, risk assessment, changes of insecticides sensitivity and fitness after selection of dimpropyridaz in A. gossypii were evaluated in present study. The results showed that dimpropyridaz exhibited high activity against 23 field populations of A. gossypii from cotton planting regions in China with LC50 values ranging from 0.37 to 8.93 mg/L, which indicating a 24.14-fold natural susceptibility variability between the most sensitive and the most selected populations. Based on the dimpropyridaz susceptibilities of 23 field populations, the sensitivity baseline of A. gossypii to dimpropyridaz was established with the LC50 value as 1.29 mg/L. Additionally, the resistance risk of A. gossypii to dimpropyridaz was evaluated by continuously selecting both a susceptible laboratory population and a field Tulufan population. After 10 selections with dimpropyridaz, no significant dimpropyridaz resistance increased in both populations, with the realized heritability were estimated as 0.01 and 0.04 for the two populations respectively, and this meant low resistance risk to dimpropyridaz of A. gossypii. In addition, the sensitivities of the dimpropyridaz selected population to imidacloprid, acetamiprid and sulfoxaflor displayed some reduction, whereas no significant changes in sensitivity to afidopyropen, carbosulfan and bifenthrin. However, the dimpropyridaz selected population showed a low fitness, with fitness as 0.73. These results provide important information for development of effective resistance management strategies of dimpropyridaz in A. gossypii.
多菌灵是一种新型杀虫剂,已登记用于防治多种蚜虫和其他刺吸式昆虫。为明确二氟吡啶虫酰胺在中国蚜虫自然种群中的药敏性和抗性风险,本研究对二氟吡啶虫酰胺在蚜虫中的药敏性基线、风险评估、药敏性变化以及选择二氟吡啶虫酰胺后的适应性进行了评价。结果表明,多菌灵对中国棉花种植区的 23 个棉铃虫田间种群具有较高的活性,半数致死浓度(LC50)在 0.37 至 8.93 毫克/升之间,表明在最敏感种群和最经选择的种群之间存在 24.14 倍的天然敏感性差异。根据 23 个田间种群对二氯丙啶脲的敏感性,确定了棉铃虫对二氯丙啶脲的敏感性基线,其 LC50 值为 1.29 mg/L。此外,通过连续选择对二氯丙啶磷敏感的实验室种群和田间吐鲁番种群,评估了棉铃虫对二氯丙啶磷的抗性风险。经过 10 次二氯丙啶脲的选择后,两个种群对二氯丙啶脲的抗性都没有明显增加,估计两个种群的遗传率分别为 0.01 和 0.04,这意味着棉铃虫对二氯丙啶脲的抗性风险较低。此外,二氟吡啶虫酰胺选育种群对吡虫啉、啶虫脒和磺胺二甲嘧啶的敏感性有所降低,而对阿维菌素、多菌灵和联苯菊酯的敏感性没有显著变化。不过,二丙烯胺嘧啶的选育群体表现出较低的适应性,适应性为 0.73。这些结果为制定有效的棉铃虫二甲戊灵抗性管理策略提供了重要信息。
{"title":"Baseline susceptibility, risk assessment of resistance, changes in insecticides sensitivity and fitness after selection of dimpropyridaz in Aphis gossypii Glover (Hemiptera: Aphididae)","authors":"Jiao Shang ,&nbsp;Haishan Wang,&nbsp;Wenyang Dong,&nbsp;Xinyu Guo,&nbsp;Jiahao Zhu,&nbsp;Pei Liang,&nbsp;Xueyan Shi","doi":"10.1016/j.pestbp.2024.106138","DOIUrl":"10.1016/j.pestbp.2024.106138","url":null,"abstract":"<div><div>Dimpropyridaz as a novel insecticide is registered for the management of many aphid species and other piercing-sucking insects. For the aim to clarify the susceptibility of natural population and the resistance risk of dimpropyridaz in <em>Aphis gossypii</em> in China, the baseline susceptibility, risk assessment, changes of insecticides sensitivity and fitness after selection of dimpropyridaz in <em>A. gossypii</em> were evaluated in present study. The results showed that dimpropyridaz exhibited high activity against 23 field populations of <em>A. gossypii</em> from cotton planting regions in China with LC<sub>50</sub> values ranging from 0.37 to 8.93 mg/L, which indicating a 24.14-fold natural susceptibility variability between the most sensitive and the most selected populations. Based on the dimpropyridaz susceptibilities of 23 field populations, the sensitivity baseline of <em>A. gossypii</em> to dimpropyridaz was established with the LC<sub>50</sub> value as 1.29 mg/L. Additionally, the resistance risk of <em>A. gossypii</em> to dimpropyridaz was evaluated by continuously selecting both a susceptible laboratory population and a field Tulufan population. After 10 selections with dimpropyridaz, no significant dimpropyridaz resistance increased in both populations, with the realized heritability were estimated as 0.01 and 0.04 for the two populations respectively, and this meant low resistance risk to dimpropyridaz of <em>A. gossypii.</em> In addition, the sensitivities of the dimpropyridaz selected population to imidacloprid, acetamiprid and sulfoxaflor displayed some reduction, whereas no significant changes in sensitivity to afidopyropen, carbosulfan and bifenthrin. However, the dimpropyridaz selected population showed a low fitness, with fitness as 0.73. These results provide important information for development of effective resistance management strategies of dimpropyridaz in <em>A. gossypii</em>.</div></div>","PeriodicalId":19828,"journal":{"name":"Pesticide Biochemistry and Physiology","volume":"205 ","pages":"Article 106138"},"PeriodicalIF":4.2,"publicationDate":"2024-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142311503","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
RNAi-mediated silencing of NlGRP3 augments the insecticidal virulence of Metarhizium anisopliae to the brown planthopper Nilaparvata lugens RNAi- 介导的 NlGRP3 沉默增强了拟黑僵菌对褐纹夜蛾 Nilaparvata lugens 的杀虫毒力
IF 4.2 1区 农林科学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2024-09-19 DOI: 10.1016/j.pestbp.2024.106141
Zhu-Long Shao, Chen-Ping Lan, Xiao-Ping Yu, Zheng-Liang Wang

The rapid development of insecticide resistance reinforces the urgent need to develop eco-friendly strategies for controlling Nilaparvata lugens (brown planthopper, BPH), the most destructive insect pest of rice. Both entomopathogens and RNA interference (RNAi) provide attractive alternatives to chemical insecticides. In this study, we demonstrated the synergistic potential of the combination use of entomopathogen- and RNAi-mediated approaches to control BPH. The β-1, 3-glucan recognition protein (βGRP) encoding gene NlGRP3 was identified and its potential role in immune defense was characterized in BPH. The open reading frame (ORF) of NlGRP3 is 1740 bp in length, encoding a 65.8 kDa protein with conserved CBM39 and GH16 domains that typically existed in the βGRP family members. NlGRP3 was shown to be differentially expressed across developmental stages and highly transcribed in the immune responsive tissues haemolymph and fat body. Topical infection with a fungal entomopathogen Metarhizium anisopliae could significantly up-regulate its expression level. RNAi-mediated silencing of NlGRP3 resulted in significantly decreased survival rate and increased susceptibility to fungal challenge in the fifth-instar BPH nymphs. The greatly enhanced mortality of NlGRP3-silenced BPH following fungal infection might be in part directly due to the immune suppression by down-regulating expressions of antimicrobial peptide genes and the imbalance of the bacterial community harboring in BPH body. Our results highly demonstrated that suppressing the insect innate immune defense through RNAi targeting the immune-related genes could effectively strengthen the biocontrol efficacy of fungal entomopathogens, providing clues to the combination use of RNAi and entomopathogens as a promising approach for BPH control.

杀虫剂抗药性的迅速发展加强了开发生态友好型战略来控制水稻最具破坏性害虫 Nilaparvata lugens(褐跳甲,BPH)的迫切需要。昆虫病原体和 RNA 干扰(RNAi)为化学杀虫剂提供了有吸引力的替代品。在这项研究中,我们证明了昆虫病原体和 RNAi 介导的方法联合使用防治 BPH 的协同潜力。研究发现了β-1, 3-葡聚糖识别蛋白(βGRP)编码基因NlGRP3,并鉴定了其在BPH免疫防御中的潜在作用。NlGRP3 的开放阅读框(ORF)长度为 1740 bp,编码 65.8 kDa 的蛋白质,具有 CBM39 和 GH16 结构域,这些结构域通常存在于 βGRP 家族成员中。研究表明,NlGRP3 在不同的发育阶段有不同的表达,并在免疫反应组织血淋巴和脂肪体中高度转录。局部感染真菌昆虫病原体Metarhizium anisopliae可显著上调其表达水平。以 RNAi- 为介导的 NlGRP3 沉默会导致第五龄 BPH 若虫的存活率明显降低,对真菌挑战的敏感性增加。被 NlGRP3 沉默的 BPH 在真菌感染后死亡率大大增加,部分直接原因可能是抗菌肽基因表达下调导致免疫抑制,以及 BPH 体内细菌群落失衡。我们的研究结果高度证明,通过RNAi靶向免疫相关基因抑制昆虫先天免疫防御,可有效增强真菌昆虫病原体的生物防治效果,为RNAi与昆虫病原体的结合使用提供了线索,是一种很有前景的防治BPH的方法。
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引用次数: 0
Investigating the role of the ROS/CncC-xenobiotics signaling pathway in the response to Fenpropathrin in Cyprinus carpio lymphocytes: Involving lipid peroxidation and Fe2+ metabolism imbalance 研究 ROS/CncC-xenobiotics 信号通路在鲤鱼淋巴细胞对苯丙菊酯反应中的作用:涉及脂质过氧化和 Fe2+ 代谢失衡
IF 4.2 1区 农林科学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2024-09-19 DOI: 10.1016/j.pestbp.2024.106142
Lei Diao , Jing Gao , Yuxun Zhou , Liping Wang , Xiaowei Yang , Peng Li , Jingying Zhai , Yang Ma , Huijie Chen

Fenpropathrin (FPT) is a synthetic pyrethroid insecticide, the persistence and accumulation in water of which could cause harmful effects on vulnerable groups like aquatic creatures, particularly posing significant risks to fish immune systems. This study aimed to investigate how environmentally relevant FPT concentrations (10–1000 μ/M) affect lipid peroxidation and Fe2+ metabolism in Cyprinus carpio head kidney lymphocytes, and its relationship with oxidative stress and immunotoxicity. Firstly, CCK-8 results demonstrated that FPT caused a significant increase in lymphocyte death. Secondly, lymphocytes exposed to FPT could lead ferroptosis in lymphocytes, accompanied by evidence of the Fe2+ transporter imbalance, lipid peroxidation, Fe2+ accumulation and ferroptosis related protein increment. Thirdly, we found that FPT esposure leads to a decrease in ATP, mitochondrial DNA and NADPH/NADP+ levels, and the mRNA associated with mitochondrial function-related genes (Fis1, Drp1, and OPA1) in lymphocytes. Additionally, FPT induced the increased the levels of inflammatory genes (TNF-α, IFN-γ, and IL-6) in head kidney lymphocytes. Importantly, exposure to FPT induced oxidative stress to produce intracellular ROS, disrupting the function of the CncC signaling pathway and expression disorder of xenobiotics detoxification (CYP 450 family) genes. Notably, Treatment with NAC (a ROS inhibitor, 5 μM) demonstrated that inhibiting ROS alleviated FPT-induced lymphocyte ferroptosis and inflammatory response via the ROS/CncC-xenobiotics signaling pathway. These findings not only introduces a novel approach to investigating the immunotoxicity of FPT but also offers critical insights into mitigating the adverse effects of FPT on aquatic animal health.

苯丙菊酯(FPT)是一种合成的拟除虫菊酯类杀虫剂,其在水中的持久性和蓄积性会对水生生物等弱势群体造成有害影响,特别是对鱼类的免疫系统构成重大风险。本研究旨在探讨环境相关浓度(10-1000 μ/M)的 FPT 如何影响鲤鱼头肾淋巴细胞的脂质过氧化和 Fe2+ 代谢,及其与氧化应激和免疫毒性的关系。首先,CCK-8 结果表明,FPT 导致淋巴细胞死亡显著增加。其次,暴露于 FPT 的淋巴细胞可导致淋巴细胞铁变态反应,并伴有 Fe2+ 转运体失衡、脂质过氧化、Fe2+ 积累和铁变态反应相关蛋白增加等证据。第三,我们发现 FPT 暴露会导致淋巴细胞中 ATP、线粒体 DNA 和 NADPH/NADP+ 水平下降,线粒体功能相关基因(Fis1、Drp1 和 OPA1)的 mRNA 也会下降。此外,FPT 还诱导头肾淋巴细胞中炎症基因(TNF-α、IFN-γ 和 IL-6)水平升高。重要的是,暴露于 FPT 会诱发氧化应激,产生细胞内 ROS,破坏 CncC 信号通路的功能和异种生物解毒(CYP 450 家族)基因的表达紊乱。值得注意的是,用 NAC(ROS 抑制剂,5 μM)治疗表明,抑制 ROS 可通过 ROS/CncC- 异种生物信号通路缓解 FPT 诱导的淋巴细胞铁蛋白沉着和炎症反应。这些发现不仅为研究 FPT 的免疫毒性提供了一种新方法,而且还为减轻 FPT 对水生动物健康的不利影响提供了重要见解。
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引用次数: 0
Knockdown of TcGluCl leads to the premature pupation of Tribolium castaneum larvae possibly by influencing the calcium-mediating hormone homeostasis 敲除 TcGluCl 可能会影响钙介导激素的平衡,从而导致蓖麻毛虫幼虫过早化蛹
IF 4.2 1区 农林科学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2024-09-18 DOI: 10.1016/j.pestbp.2024.106137
Xi Zeng , Chengyun Jiang , Xu Zhao , Zhaolu Wu , Anxiang Zhuang , Kun Qian , Jianjun Wang , Xiangkun Meng

The glutamate-gated chloride channels (GluCls) are widely existed in the neural and nonneural tissues of invertebrate. In addition to play important roles in signal transduction, the GluCls also showed multiple physiological functions in insects such as participate in the juvenile hormone synthesis. In the present study, the potential roles of TcGluCl in growth and development of the red flour beetle Tribolium castaneum were explored. Knockdown of TcGluCl showed no effects on the survivability, weight growth, final pupation rate, eclosion and fecundity of T. castaneum, whereas resulted in the significant premature pupation of larvae. Inhibition of TcGluCl expression significantly changed the levels of juvenile hormone and ecdysone as well as the expressions of hormone biosynthetic genes. The increased ecdysone level and decreased juvenile hormone level were observed at the late stage of dsGluCl-treated larvae. Knockdown of TcGluCl significantly reduced the expressions of TcSTIM1 and TcOrai1, which were the primary proteins in store-operated calcium entry (SOCE) mediated Ca2+ influx mechanism. Whilst the L-glutamic acid treatment led to the increased TcOrai1 expression in T. castaneum. These findings suggested that knockdown of TcGluCl increased the ecdysone level and contributed to the premature pupation of larvae, which might be due to the reduced Ca2+ influx caused by the decreased expressions of TcSTIM1 and TcOrai1. These studies provide novel insights on the function of GluCls in insects.

谷氨酸门控氯离子通道(GluCls)广泛存在于无脊椎动物的神经和非神经组织中。除了在信号转导中发挥重要作用外,GluCls 在昆虫体内还表现出多种生理功能,如参与幼虫激素的合成等。本研究探讨了 TcGluCl 在红粉甲虫 Tribolium castaneum 生长发育过程中的潜在作用。敲除 TcGluCl 对红粉甲虫的存活率、体重增长、最终化蛹率、羽化率和繁殖力没有影响,但会导致幼虫明显过早化蛹。抑制 TcGluCl 的表达会显著改变幼虫激素和蜕皮激素的水平以及激素生物合成基因的表达。在dsGluCl处理的幼虫后期,观察到蜕皮激素水平升高,稚虫激素水平降低。TcGluCl的敲除显著降低了TcSTIM1和TcOrai1的表达,而TcSTIM1和TcOrai1是储存操作钙离子通道(SOCE)介导的Ca2+流入机制的主要蛋白。而L-谷氨酸处理则导致TcOrai1在蓖麻中的表达增加。这些研究结果表明,TcGluCl的敲除增加了蜕皮激素水平,导致幼虫过早化蛹,这可能是由于TcSTIM1和TcOrai1的表达量减少导致Ca2+流入减少所致。这些研究为GluCls在昆虫中的功能提供了新的见解。
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引用次数: 0
Resistance monitoring of diamide insecticides and characterization of field-evolved chlorantraniliprole resistance among Chinese populations of the tomato pinworm Phthorimaea (=Tuta) absoluta (Lepidoptera: Gelechiidae) 二酰胺类杀虫剂的抗药性监测及中国番茄蛲虫(Phthorimaea (=Tuta) absoluta (鳞翅目:蝼蛄科))种群田间演化的氯虫苯甲酰胺抗药性特征描述
IF 4.2 1区 农林科学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2024-09-18 DOI: 10.1016/j.pestbp.2024.106140
Xiaoli Ma , Cheng Qu , Jiaqi Yao , Jixing Xia , Chen Luo , Raul Narciso C. Guedes , Ran Wang

The tomato pinworm, Phthorimaea (=Tuta) absoluta, is considered one of the most destructive and invasive insect pests worldwide, having developed significant resistance to many popular insecticides. In this study, we monitored the field resistance of P. absoluta populations from China to three diamide insecticides: flubendiamide, chlorantraniliprole, and cyantraniliprole. We found that one field population from Wuzhong City (WZ) exhibited high level of resistance to chlorantraniliprole. Using the WZ population and a susceptible reference strain (YN-S), we established a near-isogenic line (WZ-NIL) of P. absoluta with resistance to chlorantraniliprole. This strain also showed substantial cross-resistance to flubendiamide, and cyantraniliprole. Genetic analysis revealed that the inheritance of resistance to chlorantraniliprole in the WZ-NIL strain was autosomal and incompletely dominant. Additionally, the pesticide synergist piperonyl butoxide significantly inhibited chlorantraniliprole resistance by compromising P450 monooxygenase activity, which was significantly higher in the resistant strain. Furthermore, WZ-NIL had significantly prolonged developmental stages, lower pupation rates, reduced female fecundity, and lower egg hatchability than YN-S individuals. The fitness of WZ-NIL relative to YN-S was estimated to be 0.73, indicating significant fitness cost associated with chlorantraniliprole resistance. Rotating chlorantraniliprole with other insecticides that have different modes of action and degradation may be particularly useful for managing chlorantraniliprole resistance in P. absoluta.

番茄蛲虫(Phthorimaea (=Tuta) absoluta)被认为是全球最具破坏性和入侵性的害虫之一,对许多常用杀虫剂产生了显著的抗药性。在这项研究中,我们监测了中国的绝对蛲虫种群对氟苯虫胺、氯虫苯甲酰胺和氰虫苯甲酰胺这三种二酰胺类杀虫剂的田间抗药性。我们发现,吴忠市(WZ)的一个田间种群对氯虫苯甲酰胺表现出高度抗性。利用 WZ 群体和一个易感参比品系(YN-S),我们建立了一个具有氯虫苯甲酰胺抗性的 P. absoluta 近等基因系(WZ-NIL)。该品系还表现出对氟苯虫酰胺和氰戊菊酯的大量交叉抗性。遗传分析表明,WZ-NIL 株系对氯虫苯甲酰胺的抗性是常染色体不完全显性遗传。此外,农药增效剂胡椒基丁醚通过损害 P450 单氧化酶的活性,显著抑制了抗性菌株对氯虫苯甲酰胺的抗性。此外,与YN-S个体相比,WZ-NIL的发育阶段明显延长,化蛹率降低,雌虫受精率降低,卵孵化率降低。据估计,WZ-NIL相对于YN-S的适应性为0.73,这表明氯虫苯甲酰胺抗性带来了巨大的适应性成本。将氯虫苯甲酰胺与其他具有不同作用和降解模式的杀虫剂轮换使用,可能对控制 P. absoluta 的氯虫苯甲酰胺抗性特别有用。
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引用次数: 0
The role of neuropeptide prothoracicotropic hormone (PTTH) - Torso in pyriproxyfen-induced larval-pupal abnormal metamorphosis in silkworms 神经肽促胸腺激素(PTTH)-躯体在吡丙醚诱导的家蚕幼虫-蛹异常变态中的作用
IF 4.2 1区 农林科学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2024-09-18 DOI: 10.1016/j.pestbp.2024.106139
Chunhui He , Yizhe Li , Zhenfeng Zhou , Yuting Wei , Yizhou Zhu , Yirong Han , Yifei Li , Rifeng Yang , Kaizun Xu

The neuropeptide prothoracicotropic hormone (PTTH) plays a key role in regulating ecdysone synthesis and promoting insect metamorphosis. Pyriproxyfen is a juvenile hormone analogue. We previously reported that pyriproxyfen disrupts ecdysone secretion and inhibits larval-pupal metamorphosis in silkworms. However, the specific molecular mechanisms by which pyriproxyfen interferes with ecdysone signaling remain to be elucidated. Herein, the RNA-seq analysis on the ecdysone-secretion organ prothoracic gland (PG) was conducted following pyriproxyfen exposure. A total of 3774 differentially expressed genes (DEGs) were identified, with 1667 up-regulated and 2107 down-regulated. KEGG analysis showed that DEGs were enriched in the MAPK signaling pathway, a conserved pathway activated by PTTH binding to Torso, which regulates the ecdysone synthesis. qRT-PCR results indicated a significant up-regulation in PTTH transcription level, while the transcription levels of torso and downstream MAPK pathway genes, Ras2, Raf and ERK, were down-regulated 24 h post-pyriproxyfen treatment. Consistent with these transcriptional changes, PTTH titers in the brain also increased following pyriproxyfen treatment. These results suggest that pyriproxyfen induces abnormal metamorphosis in silkworms by impairing PTTH-Torso signaling. This study enhances our understanding of the molecular mechanisms of pyriproxyfen-induced larval-pupal abnormal metamorphosis in silkworms, and also provides insights for developing detoxification strategies for juvenile hormone analog pesticides to non-target organisms.

神经肽促胸腺激素(PTTH)在调节蜕皮激素合成和促进昆虫变态过程中起着关键作用。吡丙醚是一种幼虫激素类似物。我们以前曾报道过吡丙醚会干扰蜕皮激素的分泌,抑制家蚕幼虫-蛹的变态过程。然而,吡丙醚干扰蜕皮激素信号转导的具体分子机制仍有待阐明。在此,研究人员对暴露于吡丙醚后的蜕皮激素分泌器官前胸腺(PG)进行了RNA-seq分析。共鉴定出3774个差异表达基因(DEGs),其中1667个上调,2107个下调。qRT-PCR 结果显示 PTTH 的转录水平显著上调,而 torso 和下游 MAPK 通路基因 Ras2、Raf 和 ERK 的转录水平则在吡丙醚处理后 24 小时内下调。与这些转录变化相一致的是,吡丙醚处理后大脑中的 PTTH 滴度也增加了。这些结果表明,吡丙醚通过损害 PTTH-Torso 信号转导诱导家蚕异常变态。这项研究加深了我们对吡丙醚诱导家蚕幼虫-蛹异常变态的分子机制的理解,同时也为制定稚虫激素类似物农药对非靶标生物的解毒策略提供了启示。
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Pesticide Biochemistry and Physiology
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